CmaCh19G004190 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh19G004190
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	trypsin inhibitor 1-like
Location	Cma_Chr19: 5056669 .. 5057194 (-)
RNA-Seq Expression	CmaCh19G004190
Synteny	CmaCh19G004190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATATCAACTTTAAAATGACGTCTCTACGTAAGTATATCTTTTGGTAATGTTTTCTTAGGTTCTTCCTTTATTAATATACTTTTGGTCTTTACCTAATGCATTCTTTTTTTTTTTTTGAATGATTCAGTAAAATCATGGCCAGAACTAAATTTCATGCAGGCTAGCATGGTAGAAAATATTATAAAGAAGCAACATCCTGAATTTCAGATAGTGGTATTGTTAGCAGGAAGTCCAGTAACCAAAGATCTTAGACAGGGACGAGTTCGACTGTTTATAAATGTAGCTGGTTTTGTGGTTCAAATTCCTCACGAGGGCTAAAATGTCTTAAATATATATGTTTAAGATCATACCTAAATAAAGAATAATATTTCCACTACTAAACATATTTGTAGCTTGAGCACTAGATGGTTTATTAGTTTCTAAATTAATATTGATTTTGAGACAATAAAATATGCGAACATAAATTAATAATATATTCTATTTATAATAATTGTATCAATTCACTCTCACCTTATGATTTGTTG

mRNA sequence

AGATATCAACTTTAAAATGACGTCTCTACTAAAATCATGGCCAGAACTAAATTTCATGCAGGCTAGCATGGTAGAAAATATTATAAAGAAGCAACATCCTGAATTTCAGATAGTGGTATTGTTAGCAGGAAGTCCAGTAACCAAAGATCTTAGACAGGGACGAGTTCGACTGTTTATAAATGTAGCTGGTTTTGTGGTTCAAATTCCTCACGAGGGCTAAAATGTCTTAAATATATATGTTTAAGATCATACCTAAATAAAGAATAATATTTCCACTACTAAACATATTTGTAGCTTGAGCACTAGATGGTTTATTAGTTTCTAAATTAATATTGATTTTGAGACAATAAAATATGCGAACATAAATTAATAATATATTCTATTTATAATAATTGTATCAATTCACTCTCACCTTATGATTTGTTG

Coding sequence (CDS)

ATGACGTCTCTACTAAAATCATGGCCAGAACTAAATTTCATGCAGGCTAGCATGGTAGAAAATATTATAAAGAAGCAACATCCTGAATTTCAGATAGTGGTATTGTTAGCAGGAAGTCCAGTAACCAAAGATCTTAGACAGGGACGAGTTCGACTGTTTATAAATGTAGCTGGTTTTGTGGTTCAAATTCCTCACGAGGGCTAA

Protein sequence

MTSLLKSWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIPHEG

Homology

[Close]

BLAST of CmaCh19G004190 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.0e-09
Identity = 32/58 (55.17%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 8   WPELNFMQASMVENIIKKQHPEF-QIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 65
           WPEL  + A   + II+K++P    I +LL GSPVTKD R  RVRLF+N+ G VVQIP
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIP 108

[Close]

BLAST of CmaCh19G004190 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.9e-08
Identity = 31/63 (49.21%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 6  KSWPELNFMQASMVENIIKKQHPEF-QIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 65
          ++WPEL  + A     II+K++ +   +  +L GSPVTKD R  RVRLF+NV  FVVQIP
Sbjct: 32 ETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQIP 91

Query: 66 HEG 68
            G
Sbjct: 92 RVG 94

[Close]

BLAST of CmaCh19G004190 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.3e-08
Identity = 30/61 (49.18%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 8   WPELNFMQASMVENIIKKQHPEF-QIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIPHE 67
           WPEL    A     II+K++P+   +  +L G+PVT+DLR  RVRLF+NV  FVVQ P  
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQTPQV 106

[Close]

BLAST of CmaCh19G004190 vs. ExPASy Swiss-Prot
Match: Q03198 (Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 5.6e-08
Identity = 29/61 (47.54%), Postives = 38/61 (62.30%), Query Frame = 0


Query: 8   WPELNFMQASMVENIIKKQHPEF-QIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIPHE 67
           WPEL    A     II+K++P+   +  +L G PVT+DLR  RVRLF+NV  F+VQ P  
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTILNGGPVTEDLRCNRVRLFVNVLDFIVQTPQI 106

[Close]

BLAST of CmaCh19G004190 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.5e-08
Identity = 27/61 (44.26%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 7  SWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIPHE 66
          SWP L  +  S+ + II++Q+P  + V+L  G+PVTKD R  RVR+++N  G VV  P  
Sbjct: 8  SWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSPPRI 67

Query: 67 G 68
          G
Sbjct: 68 G 68

[Close]

BLAST of CmaCh19G004190 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.7e-15
Identity = 43/63 (68.25%), Postives = 50/63 (79.37%), Query Frame = 0


Query: 5  LKSWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 64
          LKSWP LNFM A  V NIIKK++P+FQI +LLAGSPVTKDL+ GRVRLF N+   VV +P
Sbjct: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVP 68

Query: 65 HEG 68
           EG
Sbjct: 69 QEG 71

[Close]

BLAST of CmaCh19G004190 vs. ExPASy TrEMBL
Match: A0A0A0LFD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-09
Identity = 33/63 (52.38%), Postives = 43/63 (68.25%), Query Frame = 0


Query: 5  LKSWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 64
          LKSWP L F+ A  V N IK ++P F+IV +  G+PVTKD+  GRVRL ++  G VV +P
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 65 HEG 68
           EG
Sbjct: 67 QEG 69

[Close]

BLAST of CmaCh19G004190 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.2e-08
Identity = 31/61 (50.82%), Postives = 46/61 (75.41%), Query Frame = 0


Query: 7  SWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIPHE 66
          SWPEL F+++S+  + I++  P+ + VVLLAGSPVT+DLR  RVR+F+N+   VV++P  
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 67 G 68
          G
Sbjct: 75 G 75

[Close]

BLAST of CmaCh19G004190 vs. ExPASy TrEMBL
Match: A0A1S3YJI6 (trypsin inhibitor 1-like OS=Nicotiana tabacum OX=4097 GN=LOC107776717 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.4e-07
Identity = 32/63 (50.79%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 6  KSWPELNFMQASMVENIIKKQHPEF-QIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 65
          ++WPEL  + A     II+K++P+   +  LL GSPVTKDLR  RVRLF+NV   VVQ+P
Sbjct: 32 ETWPELIGVPAKFAREIIQKENPKLTNVPSLLNGSPVTKDLRCNRVRLFVNVLDIVVQLP 91

Query: 66 HEG 68
            G
Sbjct: 92 RVG 94

[Close]

BLAST of CmaCh19G004190 vs. ExPASy TrEMBL
Match: A0A6N2ARY0 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023456 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 8.4e-07
Identity = 28/57 (49.12%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 8   WPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 65
           WPEL  +   + + II+K++P   + +LL+GSP+T D R  RVRLF N+ GFVVQ+P
Sbjct: 51  WPELIGVPTKLAKEIIEKENPLINVQILLSGSPITLDYRCDRVRLFDNILGFVVQMP 107

[Close]

BLAST of CmaCh19G004190 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 112.1 bits (279), Expect = 1.9e-21
Identity = 52/67 (77.61%), Postives = 59/67 (88.06%), Query Frame = 0


Query: 1  MTSLLKSWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFV 60
          M  +LKSWPELN MQAS VENIIK+QHPEFQIV+LLAGSPVTKDL+ GRVRLF+N+ G V
Sbjct: 1  MAYVLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIV 60

Query: 61 VQIPHEG 68
          V+IP EG
Sbjct: 61 VEIPREG 67

[Close]

BLAST of CmaCh19G004190 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 91.3 bits (225), Expect = 3.5e-15
Identity = 43/63 (68.25%), Postives = 50/63 (79.37%), Query Frame = 0


Query: 5  LKSWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 64
          LKSWP LNFM A  V NIIKK++P+FQI +LLAGSPVTKDL+ GRVRLF N+   VV +P
Sbjct: 9  LKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVP 68

Query: 65 HEG 68
           EG
Sbjct: 69 QEG 71

[Close]

BLAST of CmaCh19G004190 vs. NCBI nr
Match: KGN60478.1 (hypothetical protein Csa_001396 [Cucumis sativus])

HSP 1 Score: 72.0 bits (175), Expect = 2.2e-09
Identity = 33/63 (52.38%), Postives = 43/63 (68.25%), Query Frame = 0


Query: 5  LKSWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 64
          LKSWP L F+ A  V N IK ++P F+IV +  G+PVTKD+  GRVRL ++  G VV +P
Sbjct: 7  LKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNVP 66

Query: 65 HEG 68
           EG
Sbjct: 67 QEG 69

[Close]

BLAST of CmaCh19G004190 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 68.6 bits (166), Expect = 2.4e-08
Identity = 31/61 (50.82%), Postives = 46/61 (75.41%), Query Frame = 0


Query: 7  SWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIPHE 66
          SWPEL F+++S+  + I++  P+ + VVLLAGSPVT+DLR  RVR+F+N+   VV++P  
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 67 G 68
          G
Sbjct: 75 G 75

[Close]

BLAST of CmaCh19G004190 vs. NCBI nr
Match: XP_009590374.1 (trypsin inhibitor 1 [Nicotiana tomentosiformis] >XP_016452117.1 PREDICTED: trypsin inhibitor 1-like [Nicotiana tabacum])

HSP 1 Score: 62.8 bits (151), Expect = 1.3e-06
Identity = 32/63 (50.79%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 6  KSWPELNFMQASMVENIIKKQHPEF-QIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 65
          ++WPEL  + A     II+K++P+   +  LL GSPVTKDLR  RVRLF+NV   VVQ+P
Sbjct: 32 ETWPELIGVPAKFAREIIQKENPKLTNVPSLLNGSPVTKDLRCNRVRLFVNVLDIVVQLP 91

Query: 66 HEG 68
            G
Sbjct: 92 RVG 94

[Close]

BLAST of CmaCh19G004190 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 50.8 bits (120), Expect = 4.8e-07
Identity = 24/61 (39.34%), Postives = 34/61 (55.74%), Query Frame = 0


Query: 7  SWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIPHE 66
          SWPEL          +I++++P     V+L GSPVT D R  RVR+F++    VV+ P  
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKS 69

Query: 67 G 68
          G
Sbjct: 70 G 70

[Close]

BLAST of CmaCh19G004190 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 43.5 bits (101), Expect = 7.7e-05
Identity = 20/58 (34.48%), Postives = 36/58 (62.07%), Query Frame = 0


Query: 7  SWPELNFMQASMVENIIKKQHPEFQIVVLLAGSPVTKDLRQGRVRLFINVAGFVVQIP 65
          SWPEL   +    + +I++++P+ + V++L G+ V +     RV +++N  G VVQIP
Sbjct: 39 SWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQIP 96

The following BLAST results are available for this feature:

BLAST of CmaCh19G004190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16231	5.0e-09	55.17	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
Q02214	1.9e-08	49.21	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
Q03199	3.3e-08	49.18	Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1	[more]
Q03198	5.6e-08	47.54	Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1	[more]
P19873	9.5e-08	44.26	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]

BLAST of CmaCh19G004190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIF6	1.7e-15	68.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1	[more]
A0A0A0LFD4	1.1e-09	52.38	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1	[more]
A0A0A0KPI0	1.2e-08	50.82	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A1S3YJI6	6.4e-07	50.79	trypsin inhibitor 1-like OS=Nicotiana tabacum OX=4097 GN=LOC107776717 PE=3 SV=1	[more]
A0A6N2ARY0	8.4e-07	49.12	Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023456 PE=3 SV=1	[more]

BLAST of CmaCh19G004190 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571807.1	1.9e-21	77.61	hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN60477.1	3.5e-15	68.25	hypothetical protein Csa_001998 [Cucumis sativus]	[more]
KGN60478.1	2.2e-09	52.38	hypothetical protein Csa_001396 [Cucumis sativus]	[more]
XP_011654473.1	2.4e-08	50.82	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
XP_009590374.1	1.3e-06	50.79	trypsin inhibitor 1 [Nicotiana tomentosiformis] >XP_016452117.1 PREDICTED: tryps...	[more]

BLAST of CmaCh19G004190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	4.8e-07	39.34	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	7.7e-05	34.48	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 7..20 score: 33.54 coord: 44..54 score: 53.75 coord: 32..43 score: 48.91
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 7..65 e-value: 6.8E-15 score: 55.5
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 6..67
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 2..67 e-value: 1.6E-16 score: 62.2
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 6..67
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 4..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh19G004190.1	CmaCh19G004190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity