CmaCh19G002040 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh19G002040 vs. ExPASy Swiss-Prot
Match: Q84P24 (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=2)

HSP 1 Score: 589.7 bits (1519), Expect = 3.3e-167
Identity = 304/542 (56.09%), Postives = 392/542 (72.32%), Query Frame = 0

Query: 29  PGWYSPDTGICRSVHASRDLPTDPFLDVASYILSFQHNGRSALIDSSTGHSISYRELYRM 88
           P W+S  TGI  S   S  LP DP LD  S + S +H+G +ALIDS TG SIS+ EL  M
Sbjct: 22  PFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHKHHGDTALIDSLTGFSISHTELQIM 81

Query: 89  VNSMASGL-QKLGVSQGDVVLLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRI 148
           V SMA+G+   LGV QGDVV L+LPNS+++P++ L ++ LGA++TTM P SS  EIKK++
Sbjct: 82  VQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQV 141

Query: 149 SECNVRLAFATPQNVGNFEALGVQAIGVPENTNLDLMRPMGFSSFYELISSGFDLNKRHV 208
           SEC+V LAF + +NV    +LGV  I V E+ + D +R +    FY ++   F    + +
Sbjct: 142 SECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMKESFGFVPKPL 201

Query: 209 IRQEDTAAILFSSGTTGVSKGVLLTHRNFISTIELFVRFEASQYEYLTTENVYLAAIPMF 268
           I+Q+D AAI++SSGTTG SKGVLLTHRN I+++ELFVRFEASQYEY  + NVYLAA+P+ 
Sbjct: 202 IKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLC 261

Query: 269 HVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMAIDRFKVTHFPVVPPIMIVMARIARKFG 328
           H+YGLS+FVMGLLSLGS+IVVM +FD  +VV  I+RFK+THFPVVPP+++ + + A+   
Sbjct: 262 HIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKGVC 321

Query: 329 GHRFRSLKQVSCGAASLSMKIVGDFVQALPHVDFIQGYGMTETTAVGTRGFNTKKAQNHL 388
           G  F+SLKQVS GAA LS K + DF+Q LPHVD IQGYGMTE+TAVGTRGFN++K   + 
Sbjct: 322 GEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYS 381

Query: 389 SVGLLAPNMEAKVVDWVSASIMPPGKTGELLLRGPGLMKGYLNNPEATMSTIDQENWLHT 448
           SVGLLAPNM+AKVVDW S S +PPG  GEL ++GPG+MKGYLNNP+AT  +I +++WL T
Sbjct: 382 SVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRT 441

Query: 449 GDIAYFDRDGYLYVVDRLKEVIKYKGFQ-------------------------------I 508
           GDIAYFD DGYL++VDR+KE+IKYKGFQ                               I
Sbjct: 442 GDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEI 501

Query: 509 PVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIRKVVFTESIPKSAAGKVLRKELVKHLPS 539
           PVAFVV++  ++LS++DVI YVA QVAPY+K+RKVV   SIPKS  GK+LRKEL + L +
Sbjct: 502 PVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILRKELKRILTN 561

BLAST of CmaCh19G002040 vs. ExPASy Swiss-Prot
Match: Q6YYZ2 (4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL3 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 1.1e-130
Identity = 269/549 (49.00%), Postives = 349/549 (63.57%), Query Frame = 0

Query: 29  PGWYSPDTGICRSVHASRDLPTDPFLDVASYI---LSFQHNGRSALIDSSTGHSISYREL 88
           P +YS  TGI  S+H    LPTDP L + +++   L     G   L+D++T  ++S  +L
Sbjct: 25  PPFYSAATGIYSSIHPPVALPTDPSLTLVAHLFARLPLADPGAPTLVDAATASAVSRADL 84

Query: 89  YRMVNSMASGLQKL-GVSQGDVVLLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIK 148
            R+V S+A+GL++  GV +G VVLLLLPNS+ +P+  L VL  GAV TTM P SS  EI 
Sbjct: 85  RRLVASLAAGLRRRHGVRKGSVVLLLLPNSVAFPVSFLAVLAAGAVATTMNPSSSPAEIA 144

Query: 149 KRISECNVRLAFATPQNVGNFEALGVQAIGVPENTNLDLMRPMG------FSSFYELISS 208
            +       L  A+         L    + VPE  +       G      F++F  ++  
Sbjct: 145 AQARATGACLVLASRDGAARLPPLAAPVVLVPEILDHSAAADDGDDDQRVFAAFRAMLDG 204

Query: 209 GFDLNKR---HVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTIELFVRFEASQYEYL- 268
           G          V+ Q+D  AIL+SSGT+G SKGV+LTHRN I+  ELFVRFEASQY    
Sbjct: 205 GGGDGTETAVPVVGQDDAVAILYSSGTSGRSKGVVLTHRNLIAMTELFVRFEASQYHARG 264

Query: 269 TTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMAIDRFKVTHFPVVPP 328
             ENVY+AA+PM HVYGLS+F +GLLS+G+++VVM +FD  + V AI R+KVTH P+VPP
Sbjct: 265 ARENVYMAALPMSHVYGLSLFAVGLLSIGATVVVMRRFDAGDAVAAIGRYKVTHMPLVPP 324

Query: 329 IMIVMARIARKFG--GHRFRSLKQVSCGAASLSMKIVGDFVQALPHVDFIQGYGMTETTA 388
           IM  M R A   G    +  SL QVSCGAA ++  ++ +F+QA PHVDFIQGYGMTE+TA
Sbjct: 325 IMAAMVRAAAAGGVPPSQVASLVQVSCGAAPITAALIHEFLQAFPHVDFIQGYGMTESTA 384

Query: 389 VGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLRGPGLMKGYLNNP 448
           VGTRGFNT K + + SVGLLAPNM AK+V   S+S +PPG +GEL L GPG+MKGYL++ 
Sbjct: 385 VGTRGFNTSKHKKYTSVGLLAPNMHAKIVHLESSSCLPPGFSGELWLHGPGIMKGYLSDD 444

Query: 449 EATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----------------- 508
           +   +   ++ WL TGDIAYFD DGYLY+V RLK+ IKYKGFQ                 
Sbjct: 445 DDACTR--KDGWLRTGDIAYFDLDGYLYIVGRLKDTIKYKGFQIAPGDLEEVLIHHPEIL 504

Query: 509 --------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIRKVVFTESIPKSA 531
                         IPVAFVV++ GS+LS K V++YVA+QVAPYK++RKVVF E+IPKS 
Sbjct: 505 DVAVTSAEDEEAGEIPVAFVVRRSGSNLSCKQVMEYVAKQVAPYKRVRKVVFVEAIPKSP 564

BLAST of CmaCh19G002040 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 5.1e-112
Identity = 216/541 (39.93%), Postives = 340/541 (62.85%), Query Frame = 0

Query: 32  YSPDTGICRSVHASRDLPTDPFLDVASYILSFQHNGRSALIDSSTGHSISYRELYRMVNS 91
           Y     I  S      LP +  +DV ++I S  H+G+ A ID++TG  +++ +L+R V+S
Sbjct: 15  YCKSNSIFYSKREPVQLPQNHSVDVTTFISSRAHHGKIAFIDAATGRHLTFPQLWRAVDS 74

Query: 92  MASGLQKLGVSQGDVVLLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECN 151
           +A+ L  +G+ +GDV+LLL PNSI++P+V L V+ LGA+ITT  P ++ +EI K+I++  
Sbjct: 75  VATCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQITDSK 134

Query: 152 VRLAFATPQNVGNFEALGVQAIGVPENTNLDLMRPMGF-SSFYELISSGFDLNK-RHVIR 211
             LAF  PQ V       +  + + +     L + +   SS  E++      N+  + + 
Sbjct: 135 PVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPNRIGYRVN 194

Query: 212 QEDTAAILFSSGTTGVSKGVLLTHRNFISTIELFVRFEASQYEYLTTENVYLAAIPMFHV 271
           QEDTA +L+SSGTTG SKGV+ +H+N I+ ++  +    S++     E+ ++  +PMFH+
Sbjct: 195 QEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTIL----SRFGTEDGEHTFICTVPMFHI 254

Query: 272 YGLSIFVMGLLSLGSSIVVMSKFDVKEVVMAIDRFKVTHFPVVPPIMIVMARIARKF-GG 331
           YGL+ F MGLLS GS+IV++SKF++ E++ AI++++ T+ P+VPPI++ + + A      
Sbjct: 255 YGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANHIRAK 314

Query: 332 HRFRSLKQVSCGAASLSMKIVGDFVQALPHVDFIQGYGMTETTAVGTRGFNTKKAQNHLS 391
           +   SL+ V  G A LS +++  FV+  P V  +QGYG+TE+T +G      ++++ + +
Sbjct: 315 YDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESRRYGT 374

Query: 392 VGLLAPNMEAKVVDWVSASIMPPGKTGELLLRGPGLMKGYLNNPEATMSTIDQENWLHTG 451
            G+L+P+MEAK+V+  +   +   +TGEL LRGP +MKGY +N EAT STID E WL TG
Sbjct: 375 AGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEGWLRTG 434

Query: 452 DIAYFDRDGYLYVVDRLKEVIKYKGFQI-------------------------------P 511
           D+ Y D DG+++VVDRLKE+IKYKG+Q+                               P
Sbjct: 435 DLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKEAGQFP 494

Query: 512 VAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIRKVVFTESIPKSAAGKVLRKELVKHLPSK 539
           +A+VV+K GS+LS+  V+D++A+ VAPYK+IRKV F  SIPK+ +GK+LRK+L+K   SK
Sbjct: 495 MAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLIKLATSK 551

BLAST of CmaCh19G002040 vs. ExPASy Swiss-Prot
Match: Q336M7 (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 404.1 bits (1037), Expect = 2.5e-111
Identity = 234/513 (45.61%), Postives = 316/513 (61.60%), Query Frame = 0

Query: 31  WYSPDTGICRSVHASRDLPTDPFLDVASYILS-FQHNGRSALIDSSTGHSISYRELYRMV 90
           +YS  TG+  S+H    LP+DP L +  ++ S    +  S L+D+ T  ++S  +  R+V
Sbjct: 15  FYSAATGLYSSLHPPLPLPSDPSLSLVPHLFSHLPLHHHSLLVDAPTAATLSCADFRRLV 74

Query: 91  NSMASGL-QKLGVSQGDVVLLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRIS 150
           +S+A+GL ++L +++G +VLLLLPNS+ +P+  L VL  GAV TTM P S+  EI  R+ 
Sbjct: 75  SSLAAGLRRRLHIARGSLVLLLLPNSLTFPVAFLAVLATGAVATTMNPSSAPAEIAARLR 134

Query: 151 ECNVRLAFATPQNVGNFEALGVQAIGVPENTNLDLMRPMGFSSFYELISSGFD--LNKRH 210
           +    L  A+  N      L    + VP+              F+ L+ +  +  +    
Sbjct: 135 DTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFDFFFHALLETDPETPVEMGV 194

Query: 211 VIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTIELFVRFEASQYEYLTTENVYLAAIPM 270
            + Q+D AA+L+SSGT+G SKGV++THRN I+ +ELFVRFEASQY     +NVYLAA+PM
Sbjct: 195 GVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFEASQYTRPARDNVYLAALPM 254

Query: 271 FHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMAIDRFKVTHFPVVPPIMIVMARIARKF 330
           FHVYGLS+F +GLLSLG ++VVM +F+V + V AI ++KVTH P+VPPIM  + R     
Sbjct: 255 FHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVTHLPLVPPIMSALLRANPPL 314

Query: 331 GGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVDFIQGYGMTETTAVGTRGFNTKKAQNH 390
                 SL QVS GAA L+  ++  F+ A PHVDFIQGYGMTE+TAVGTRGFNT K + +
Sbjct: 315 ---ELDSLLQVSSGAAPLNHTLIHHFLHAFPHVDFIQGYGMTESTAVGTRGFNTCKHKKY 374

Query: 391 LSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLRGPGLMK------GYLNNPEATMSTID 450
            SVGLLAPNM AK+V   S S +PPG  GEL L GP +MK      GYLN+ +   +   
Sbjct: 375 ASVGLLAPNMHAKIVHLESGSCLPPGSYGELWLHGPAIMKEFCFVTGYLNDDDDAFTR-- 434

Query: 451 QENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ------------------------- 503
           ++ WL TGDIAYFD DGYL++V RLK+ IKYKGFQ                         
Sbjct: 435 KDGWLRTGDIAYFDSDGYLFIVGRLKDTIKYKGFQIAPADLEAVLIRHPEIIDVAVTSDE 494

BLAST of CmaCh19G002040 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 401.7 bits (1031), Expect = 1.3e-110
Identity = 216/519 (41.62%), Postives = 327/519 (63.01%), Query Frame = 0

Query: 48  LPTDPFLDVASYILSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVV 107
           LP +P LDV ++I S  H GR A ID+STG ++++ EL+R V S+A  L ++G+ +G VV
Sbjct: 26  LPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVV 85

Query: 108 LLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEA 167
           LLL PNSI +P+V L V+ LGA+ITT  P ++S EI K+I + N  LAF T Q +    A
Sbjct: 86  LLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISA 145

Query: 168 LGVQ-AIGVPENTNLDLMRPMGFSSFYELISSGFDLNK-RHVIRQEDTAAILFSSGTTGV 227
              +  I + +   +D +  +      E++      N+ +  + Q+DTA +L+SSGTTG+
Sbjct: 146 AAKKLPIVLMDEERVDSVGDV--RRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGM 205

Query: 228 SKGVLLTHRNFISTIELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSS 287
           SKGV+ +HRN I+ ++  V    S       E  ++  +PMFH+YGL+ F  GLL+ GS+
Sbjct: 206 SKGVISSHRNLIAMVQTIVNRFGSD----DGEQRFICTVPMFHIYGLAAFATGLLAYGST 265

Query: 288 IVVMSKFDVKEVVMAIDRFKVTHFPVVPPIMIVMARIARKF-GGHRFRSLKQVSCGAASL 347
           I+V+SKF++ E++ AI +++ T  P+VPPI++ M   A +    +   S+  V CG A L
Sbjct: 266 IIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPL 325

Query: 348 SMKIVGDFVQALPHVDFIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWV 407
           S ++   F +  P V  +QGYG+TE+T +G      ++++ + + G L+ +ME ++VD V
Sbjct: 326 SKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPV 385

Query: 408 SASIMPPGKTGELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDR 467
           +  I+ P +TGEL L+GP +MKGY +N EAT ST+D E WL TGD+ Y D DG+++VVDR
Sbjct: 386 TGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDR 445

Query: 468 LKEVIKYKGFQI-------------------------------PVAFVVKKPGSSLSQKD 527
           LKE+IKYKG+Q+                               P+A+VV+K GSSLS+K 
Sbjct: 446 LKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKT 505

Query: 528 VIDYVAQQVAPYKKIRKVVFTESIPKSAAGKVLRKELVK 533
           ++++VA+QVAPYK+IRKV F  SIPK+ +GK+LRK+L+K
Sbjct: 506 IMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 538

BLAST of CmaCh19G002040 vs. ExPASy TrEMBL
Match: A0A6J1HUG7 (4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466820 PE=4 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.5e-300
Identity = 538/569 (94.55%), Postives = 538/569 (94.55%), Query Frame = 0

Query: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60
           MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI
Sbjct: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60

Query: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120
           LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV
Sbjct: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120

Query: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180
           LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN
Sbjct: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180

Query: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240
           LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI
Sbjct: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240

Query: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300
           ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA
Sbjct: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300

Query: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360
           IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD
Sbjct: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360

Query: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420
           FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR
Sbjct: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420

Query: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----- 480
           GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ     
Sbjct: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQIAPTD 480

Query: 481 --------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 539
                                     IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR
Sbjct: 481 LESVVITHPEVLDTAVAAAVDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 540

BLAST of CmaCh19G002040 vs. ExPASy TrEMBL
Match: A0A6J1HQ86 (4-coumarate--CoA ligase-like 6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466820 PE=4 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.5e-300
Identity = 538/569 (94.55%), Postives = 538/569 (94.55%), Query Frame = 0

Query: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60
           MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI
Sbjct: 5   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 64

Query: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120
           LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV
Sbjct: 65  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 124

Query: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180
           LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN
Sbjct: 125 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 184

Query: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240
           LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI
Sbjct: 185 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 244

Query: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300
           ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA
Sbjct: 245 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 304

Query: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360
           IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD
Sbjct: 305 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 364

Query: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420
           FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR
Sbjct: 365 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 424

Query: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----- 480
           GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ     
Sbjct: 425 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQIAPTD 484

Query: 481 --------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 539
                                     IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR
Sbjct: 485 LESVVITHPEVLDTAVAAAVDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 544

BLAST of CmaCh19G002040 vs. ExPASy TrEMBL
Match: A0A6J1HGK9 (4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464107 PE=4 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.6e-292
Identity = 523/569 (91.92%), Postives = 528/569 (92.79%), Query Frame = 0

Query: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60
           MAPLLNDQFSFQTPESQ VQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI
Sbjct: 1   MAPLLNDQFSFQTPESQTVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60

Query: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120
           LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV
Sbjct: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120

Query: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180
           LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEA GVQAIGVPENTN
Sbjct: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQAIGVPENTN 180

Query: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240
           LDLMRPMGFSSFYELIS GFDLNKR VIRQEDTAAILFSSGTTGVSKGVLLTHRNFIST+
Sbjct: 181 LDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTV 240

Query: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300
           ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVV+A
Sbjct: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVVA 300

Query: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360
           IDRFKVTHFPVVPPIMIVMAR A KFGGH+FRSLKQVSCGAASLS KI+GDFVQALPHVD
Sbjct: 301 IDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFVQALPHVD 360

Query: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420
           FIQGYGMTETTAVGTRGFNTKKAQN+LSVGLLAPNMEAKVVDWVS SIMPPGKTGELLLR
Sbjct: 361 FIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTGELLLR 420

Query: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----- 480
           GPGLMKGYLNNPEATMSTIDQENWLHTGDI YFDRDGYLYVVDRLKEVIKYKGFQ     
Sbjct: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTD 480

Query: 481 --------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 539
                                     IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR
Sbjct: 481 LESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 540

BLAST of CmaCh19G002040 vs. ExPASy TrEMBL
Match: A0A6J1HHI8 (4-coumarate--CoA ligase-like 6 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464107 PE=4 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.6e-292
Identity = 523/569 (91.92%), Postives = 528/569 (92.79%), Query Frame = 0

Query: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60
           MAPLLNDQFSFQTPESQ VQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI
Sbjct: 5   MAPLLNDQFSFQTPESQTVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 64

Query: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120
           LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV
Sbjct: 65  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 124

Query: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180
           LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEA GVQAIGVPENTN
Sbjct: 125 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQAIGVPENTN 184

Query: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240
           LDLMRPMGFSSFYELIS GFDLNKR VIRQEDTAAILFSSGTTGVSKGVLLTHRNFIST+
Sbjct: 185 LDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTV 244

Query: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300
           ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVV+A
Sbjct: 245 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVVA 304

Query: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360
           IDRFKVTHFPVVPPIMIVMAR A KFGGH+FRSLKQVSCGAASLS KI+GDFVQALPHVD
Sbjct: 305 IDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFVQALPHVD 364

Query: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420
           FIQGYGMTETTAVGTRGFNTKKAQN+LSVGLLAPNMEAKVVDWVS SIMPPGKTGELLLR
Sbjct: 365 FIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTGELLLR 424

Query: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----- 480
           GPGLMKGYLNNPEATMSTIDQENWLHTGDI YFDRDGYLYVVDRLKEVIKYKGFQ     
Sbjct: 425 GPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTD 484

Query: 481 --------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 539
                                     IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR
Sbjct: 485 LESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 544

BLAST of CmaCh19G002040 vs. ExPASy TrEMBL
Match: A0A5D3DC80 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00450 PE=4 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 1.0e-248
Identity = 447/575 (77.74%), Postives = 487/575 (84.70%), Query Frame = 0

Query: 1   MAPLL-NDQFSFQTPESQAVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFL 60
           MA LL NDQFSF+TPE++A  TKKIP     NKYP WYSPDTGIC SVH  R LP+DPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVASYILS--FQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLP 120
           DV S+I S  FQHNG SALIDS TG+SISY+EL+ MV SMASGL  LG+SQGDVVLL+LP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQA 180
           NSIFYPI++LG LYLGAVITTMFPQSSS EIKKRI++CNVRLAF   Q + NFEALG++ 
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNLDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLT 240
           IGVPENTN DLMRPMGFSSFYELISSG DL KR VIRQEDTAAILFSSGTTGVSKGV+L+
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKF 300
           HRNFISTIELFVRFEASQYEYL T+NVYLAAIPMFH+YGLSIFVMGL+SLGSS+VVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKEVVMAIDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDF 360
           DVK+VV AIDRFKVTHFPVVPPI+I ++R A K G  RFRSLKQVSCGAA+ S K + DF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VQALPHVDFIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPG 420
           VQAL HVDFIQGYGMTE+TA+GTRGFN KK +N+LSVGLLAPN EAKVVDWV+ S MPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPG MKGYLNNPEATMSTIDQENWLHTGDI YFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQ-------------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQ 537
           GFQ                               IPVAFVVKKPGS+LSQKDV+DYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

BLAST of CmaCh19G002040 vs. NCBI nr
Match: XP_022967223.1 (4-coumarate--CoA ligase-like 6 isoform X2 [Cucurbita maxima] >XP_022967224.1 4-coumarate--CoA ligase-like 6 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.1e-300
Identity = 538/569 (94.55%), Postives = 538/569 (94.55%), Query Frame = 0

Query: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60
           MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI
Sbjct: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60

Query: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120
           LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV
Sbjct: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120

Query: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180
           LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN
Sbjct: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180

Query: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240
           LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI
Sbjct: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240

Query: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300
           ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA
Sbjct: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300

Query: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360
           IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD
Sbjct: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360

Query: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420
           FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR
Sbjct: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420

Query: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----- 480
           GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ     
Sbjct: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQIAPTD 480

Query: 481 --------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 539
                                     IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR
Sbjct: 481 LESVVITHPEVLDTAVAAAVDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 540

BLAST of CmaCh19G002040 vs. NCBI nr
Match: XP_022967222.1 (4-coumarate--CoA ligase-like 6 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.1e-300
Identity = 538/569 (94.55%), Postives = 538/569 (94.55%), Query Frame = 0

Query: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60
           MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI
Sbjct: 5   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 64

Query: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120
           LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV
Sbjct: 65  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 124

Query: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180
           LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN
Sbjct: 125 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 184

Query: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240
           LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI
Sbjct: 185 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 244

Query: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300
           ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA
Sbjct: 245 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 304

Query: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360
           IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD
Sbjct: 305 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 364

Query: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420
           FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR
Sbjct: 365 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 424

Query: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----- 480
           GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ     
Sbjct: 425 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQIAPTD 484

Query: 481 --------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 539
                                     IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR
Sbjct: 485 LESVVITHPEVLDTAVAAAVDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 544

BLAST of CmaCh19G002040 vs. NCBI nr
Match: KAG7011356.1 (4-coumarate--CoA ligase-like 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.8e-292
Identity = 524/569 (92.09%), Postives = 529/569 (92.97%), Query Frame = 0

Query: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60
           MAPLLNDQFSFQTPESQ VQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI
Sbjct: 5   MAPLLNDQFSFQTPESQTVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 64

Query: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120
           LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV
Sbjct: 65  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 124

Query: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180
           LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN
Sbjct: 125 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 184

Query: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240
           LDLMRPMGFSSFYELIS GFDLNKR VIRQEDTAAILFSSGTTGVSKGVLLTHRNFIST+
Sbjct: 185 LDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTV 244

Query: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300
           ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVV+A
Sbjct: 245 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVVA 304

Query: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360
           IDRFKVTHFPVVPPIMIVMAR A KFGGH+FRSLKQVSCGAASLS KI+GDFVQALPHVD
Sbjct: 305 IDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFVQALPHVD 364

Query: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420
           FIQGYGMTETTAVGTRGFNTKKAQN+LSVGLLAPNMEAKVVDWVS SIMPPGKTGELLLR
Sbjct: 365 FIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTGELLLR 424

Query: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----- 480
           GPGLMKGYLNNPEATMSTIDQENWLHTGDI YFDRDGYLYVVDRLKEVIKYKGFQ     
Sbjct: 425 GPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTD 484

Query: 481 --------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 539
                                     IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR
Sbjct: 485 LESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 544

BLAST of CmaCh19G002040 vs. NCBI nr
Match: KAG6571628.1 (4-coumarate--CoA ligase-like 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.4e-292
Identity = 523/569 (91.92%), Postives = 529/569 (92.97%), Query Frame = 0

Query: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60
           MAPLLNDQFSFQTPESQ VQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI
Sbjct: 43  MAPLLNDQFSFQTPESQTVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 102

Query: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120
           LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV
Sbjct: 103 LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 162

Query: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180
           LLGVLYLGAVITTMFP+SSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN
Sbjct: 163 LLGVLYLGAVITTMFPRSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 222

Query: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240
           LDLMRPMGFSSFYELIS GFDLNKR VIRQEDTAAILFSSGTTGVSKGVLLTHRNFIST+
Sbjct: 223 LDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTV 282

Query: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300
           ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVV+A
Sbjct: 283 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVVA 342

Query: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360
           IDRFKVTHFPVVPPIMIVMAR A KFGGH+FRSLKQVSCGAASLS KI+GDFVQALPHVD
Sbjct: 343 IDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFVQALPHVD 402

Query: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420
           FIQGYGMTETTAVGTRGFNTKKAQN+LSVGLLAPNMEAKVVDWVS SIMPPGKTGELLLR
Sbjct: 403 FIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTGELLLR 462

Query: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----- 480
           GPGLMKGYLNNPEATMSTIDQENWLHTGDI YFDRDGYLYVVDRLKEVIKYKGFQ     
Sbjct: 463 GPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTD 522

Query: 481 --------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 539
                                     IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR
Sbjct: 523 LESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 582

BLAST of CmaCh19G002040 vs. NCBI nr
Match: XP_022963967.1 (4-coumarate--CoA ligase-like 6 isoform X2 [Cucurbita moschata] >XP_022963968.1 4-coumarate--CoA ligase-like 6 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.4e-292
Identity = 523/569 (91.92%), Postives = 528/569 (92.79%), Query Frame = 0

Query: 1   MAPLLNDQFSFQTPESQAVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60
           MAPLLNDQFSFQTPESQ VQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI
Sbjct: 1   MAPLLNDQFSFQTPESQTVQTKKIPNKYPGWYSPDTGICRSVHASRDLPTDPFLDVASYI 60

Query: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120
           LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV
Sbjct: 61  LSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPNSIFYPIV 120

Query: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEALGVQAIGVPENTN 180
           LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEA GVQAIGVPENTN
Sbjct: 121 LLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQAIGVPENTN 180

Query: 181 LDLMRPMGFSSFYELISSGFDLNKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTI 240
           LDLMRPMGFSSFYELIS GFDLNKR VIRQEDTAAILFSSGTTGVSKGVLLTHRNFIST+
Sbjct: 181 LDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTV 240

Query: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMA 300
           ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVV+A
Sbjct: 241 ELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVVA 300

Query: 301 IDRFKVTHFPVVPPIMIVMARIARKFGGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVD 360
           IDRFKVTHFPVVPPIMIVMAR A KFGGH+FRSLKQVSCGAASLS KI+GDFVQALPHVD
Sbjct: 301 IDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFVQALPHVD 360

Query: 361 FIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLR 420
           FIQGYGMTETTAVGTRGFNTKKAQN+LSVGLLAPNMEAKVVDWVS SIMPPGKTGELLLR
Sbjct: 361 FIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGKTGELLLR 420

Query: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQ----- 480
           GPGLMKGYLNNPEATMSTIDQENWLHTGDI YFDRDGYLYVVDRLKEVIKYKGFQ     
Sbjct: 421 GPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTD 480

Query: 481 --------------------------IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 539
                                     IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR
Sbjct: 481 LESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIR 540

BLAST of CmaCh19G002040 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 589.7 bits (1519), Expect = 2.3e-168
Identity = 304/542 (56.09%), Postives = 392/542 (72.32%), Query Frame = 0

Query: 29  PGWYSPDTGICRSVHASRDLPTDPFLDVASYILSFQHNGRSALIDSSTGHSISYRELYRM 88
           P W+S  TGI  S   S  LP DP LD  S + S +H+G +ALIDS TG SIS+ EL  M
Sbjct: 22  PFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFSHKHHGDTALIDSLTGFSISHTELQIM 81

Query: 89  VNSMASGL-QKLGVSQGDVVLLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRI 148
           V SMA+G+   LGV QGDVV L+LPNS+++P++ L ++ LGA++TTM P SS  EIKK++
Sbjct: 82  VQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQV 141

Query: 149 SECNVRLAFATPQNVGNFEALGVQAIGVPENTNLDLMRPMGFSSFYELISSGFDLNKRHV 208
           SEC+V LAF + +NV    +LGV  I V E+ + D +R +    FY ++   F    + +
Sbjct: 142 SECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSIMKESFGFVPKPL 201

Query: 209 IRQEDTAAILFSSGTTGVSKGVLLTHRNFISTIELFVRFEASQYEYLTTENVYLAAIPMF 268
           I+Q+D AAI++SSGTTG SKGVLLTHRN I+++ELFVRFEASQYEY  + NVYLAA+P+ 
Sbjct: 202 IKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLC 261

Query: 269 HVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMAIDRFKVTHFPVVPPIMIVMARIARKFG 328
           H+YGLS+FVMGLLSLGS+IVVM +FD  +VV  I+RFK+THFPVVPP+++ + + A+   
Sbjct: 262 HIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKGVC 321

Query: 329 GHRFRSLKQVSCGAASLSMKIVGDFVQALPHVDFIQGYGMTETTAVGTRGFNTKKAQNHL 388
           G  F+SLKQVS GAA LS K + DF+Q LPHVD IQGYGMTE+TAVGTRGFN++K   + 
Sbjct: 322 GEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYS 381

Query: 389 SVGLLAPNMEAKVVDWVSASIMPPGKTGELLLRGPGLMKGYLNNPEATMSTIDQENWLHT 448
           SVGLLAPNM+AKVVDW S S +PPG  GEL ++GPG+MKGYLNNP+AT  +I +++WL T
Sbjct: 382 SVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRT 441

Query: 449 GDIAYFDRDGYLYVVDRLKEVIKYKGFQ-------------------------------I 508
           GDIAYFD DGYL++VDR+KE+IKYKGFQ                               I
Sbjct: 442 GDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEI 501

Query: 509 PVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIRKVVFTESIPKSAAGKVLRKELVKHLPS 539
           PVAFVV++  ++LS++DVI YVA QVAPY+K+RKVV   SIPKS  GK+LRKEL + L +
Sbjct: 502 PVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILRKELKRILTN 561

BLAST of CmaCh19G002040 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 401.7 bits (1031), Expect = 9.0e-112
Identity = 216/519 (41.62%), Postives = 327/519 (63.01%), Query Frame = 0

Query: 48  LPTDPFLDVASYILSFQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVV 107
           LP +P LDV ++I S  H GR A ID+STG ++++ EL+R V S+A  L ++G+ +G VV
Sbjct: 26  LPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVV 85

Query: 108 LLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEA 167
           LLL PNSI +P+V L V+ LGA+ITT  P ++S EI K+I + N  LAF T Q +    A
Sbjct: 86  LLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISA 145

Query: 168 LGVQ-AIGVPENTNLDLMRPMGFSSFYELISSGFDLNK-RHVIRQEDTAAILFSSGTTGV 227
              +  I + +   +D +  +      E++      N+ +  + Q+DTA +L+SSGTTG+
Sbjct: 146 AAKKLPIVLMDEERVDSVGDV--RRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGM 205

Query: 228 SKGVLLTHRNFISTIELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSS 287
           SKGV+ +HRN I+ ++  V    S       E  ++  +PMFH+YGL+ F  GLL+ GS+
Sbjct: 206 SKGVISSHRNLIAMVQTIVNRFGSD----DGEQRFICTVPMFHIYGLAAFATGLLAYGST 265

Query: 288 IVVMSKFDVKEVVMAIDRFKVTHFPVVPPIMIVMARIARKF-GGHRFRSLKQVSCGAASL 347
           I+V+SKF++ E++ AI +++ T  P+VPPI++ M   A +    +   S+  V CG A L
Sbjct: 266 IIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPL 325

Query: 348 SMKIVGDFVQALPHVDFIQGYGMTETTAVGTRGFNTKKAQNHLSVGLLAPNMEAKVVDWV 407
           S ++   F +  P V  +QGYG+TE+T +G      ++++ + + G L+ +ME ++VD V
Sbjct: 326 SKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPV 385

Query: 408 SASIMPPGKTGELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIAYFDRDGYLYVVDR 467
           +  I+ P +TGEL L+GP +MKGY +N EAT ST+D E WL TGD+ Y D DG+++VVDR
Sbjct: 386 TGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDR 445

Query: 468 LKEVIKYKGFQI-------------------------------PVAFVVKKPGSSLSQKD 527
           LKE+IKYKG+Q+                               P+A+VV+K GSSLS+K 
Sbjct: 446 LKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKT 505

Query: 528 VIDYVAQQVAPYKKIRKVVFTESIPKSAAGKVLRKELVK 533
           ++++VA+QVAPYK+IRKV F  SIPK+ +GK+LRK+L+K
Sbjct: 506 IMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 538

BLAST of CmaCh19G002040 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 371.7 bits (953), Expect = 9.9e-103
Identity = 217/549 (39.53%), Postives = 322/549 (58.65%), Query Frame = 0

Query: 32  YSPDTGICRSVHASRDLPTDPFLDVASYILSFQHNGRSALIDSSTGHSISYRELYRMVNS 91
           +   T I  S      LP + FLDV S+I S  H G++  +D+ TG  +S+ EL+  V  
Sbjct: 27  FCESTSIFYSKREPMALPPNQFLDVTSFIASQPHRGKTVFVDAVTGRRLSFPELWLGVER 86

Query: 92  MASGLQKLGVSQGDVVLLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECN 151
           +A  L  LGV +G+VV++L PNSI +PIV L V+ LGA+ITT  P ++S EI K+I +  
Sbjct: 87  VAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGDSR 146

Query: 152 VRLAFATPQNVG--------NFEALGVQAIGVPENTNLDLMRPMG-FSSFYELISSGFDL 211
             LAF T + V         N   + +    VP  +  D ++ +G   +  E   S   +
Sbjct: 147 PVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEPSESRV 206

Query: 212 NKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTIELF-VRFEASQYEYLTTENVYL 271
            +R  + Q+DTAA+L+SSGTTG SKGV+L+HRN I+ ++ +  RF   Q    T      
Sbjct: 207 KQR--VNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRTICT------ 266

Query: 272 AAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMAIDRFKVTHFPVVPPIMIVMAR 331
             IPM H++G   F  GL++LG +IVV+ KFD+ +++ A++  + ++  +VPPI++ M  
Sbjct: 267 --IPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVN 326

Query: 332 IARKFGG-HRFRSLKQVSCGAASLSMKIVGDFVQALPHVDFIQGYGMTETTAVGTRGFNT 391
            A +    +   SL  V  G A LS ++   FV+  P V  +QGYG+TE+TA+    FN 
Sbjct: 327 GANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNK 386

Query: 392 KKAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLRGPGLMKGYLNNPEATMSTID 451
           ++ + + + GLLAPN+E K+VD  +  ++   +TGEL +R P +MKGY  N EAT STID
Sbjct: 387 EETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTID 446

Query: 452 QENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQI------------------------ 511
            E WL TGD+ Y D DG+++VVDRLKE+IK  G+Q+                        
Sbjct: 447 SEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIP 506

Query: 512 -------PVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIRKVVFTESIPKSAAGKVLRKE 539
                  P+A++V+K GS+LS+ +++ +VA+QV+PYKKIRKV F  SIPK+ +GK+LR+E
Sbjct: 507 DMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRRE 565

BLAST of CmaCh19G002040 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 359.4 bits (921), Expect = 5.1e-99
Identity = 208/548 (37.96%), Postives = 325/548 (59.31%), Query Frame = 0

Query: 34  PDTGICRS---VHASR---DLPTDPFLDVASYILSFQHNGRSALIDSSTGHSISYRELYR 93
           P +G C+S    ++ R    LP +   DV ++I S  H G++A ID++TG  +++ +L+R
Sbjct: 12  PRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDLWR 71

Query: 94  MVNSMASGL-QKLGVSQGDVVLLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKR 153
            V+ +A  L  ++G+ +GDVVL+L PNSIF P+V L V+ LGAV TT    ++S EI K+
Sbjct: 72  AVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQ 131

Query: 154 ISECNVRLAFATPQNVGNFEALGVQAIGVPENTNLDL---MRPMG-FSSFYELISSGFDL 213
           I++ N  L F T Q       + +  +   +    +L   +R +G  S   +   SG  +
Sbjct: 132 IADSNPTLVFTTRQLAPKL-PVAISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRV 191

Query: 214 NKRHVIRQEDTAAILFSSGTTGVSKGVLLTHRNFISTIELFVRFEASQYEYLTTENVYLA 273
             R  + Q+DTA +L+SSGTTG SKGV+ +HRN  + +  F+       + L  +++++ 
Sbjct: 192 RDR--VNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS------DNLKRDDIFIC 251

Query: 274 AIPMFHVYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMAIDRFKVTHFPVVPPIMIVMARI 333
            +PMFH YGL  F MG ++LGS++V++ +F + +++ A+++ + T   + PP+++ M   
Sbjct: 252 TVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMIND 311

Query: 334 ARKF-GGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVDFIQGYGMTETTAVGTRGFNTK 393
           A      +   SLK V CG A LS ++   F++  P VD +QGY +TE+   G    + +
Sbjct: 312 ADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAE 371

Query: 394 KAQNHLSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLRGPGLMKGYLNNPEATMSTIDQ 453
           +++ + + G L  ++EA++VD  +   M   +TGEL L+GP + KGY  N EAT  TI+ 
Sbjct: 372 ESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINL 431

Query: 454 ENWLHTGDIAYFDRDGYLYVVDRLKEVIKYKGFQI------------------------- 513
           E WL TGD+ Y D DG+L+VVDRLKE+IKYKG+Q+                         
Sbjct: 432 EGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPD 491

Query: 514 ------PVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIRKVVFTESIPKSAAGKVLRKEL 539
                 P+A+VV+K  S+LS+K VID++++QVAPYKKIRKV F  SIPK+A+GK LRK+L
Sbjct: 492 KEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDL 550

BLAST of CmaCh19G002040 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 355.9 bits (912), Expect = 5.6e-98
Identity = 201/543 (37.02%), Postives = 318/543 (58.56%), Query Frame = 0

Query: 37  GICRSVHASRDLPTDPFLDVASYIL--SFQHNGRSALIDSSTGHSISYRELYRMVNSMAS 96
           GI RS+  +  LP DP   + S++   S  +  + A+ DS TG S+++ +L   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 97  GLQKLGVSQGDVVLLLLPNSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRL 156
           G  +LG+ + DVVL+  PNS  +P+  L V  +G V TT  P  +  E+ K+I + N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 157 AFATPQNVGNFEALGVQAIGVPENTNLDLMRPMGFSSFYELISSGFDLNKR------HVI 216
             +  Q     +   +  + +     +++  P G +S      +  +L++         I
Sbjct: 130 IISVNQLFDKIKGFDLPVVLLGSKDTVEI--PPGSNSKILSFDNVMELSEPVSEYPFVEI 189

Query: 217 RQEDTAAILFSSGTTGVSKGVLLTHRNFISTIELFVRFEASQYEYLTTENVYLAAIPMFH 276
           +Q DTAA+L+SSGTTG SKGV LTH NFI+   +    +    EY     V+L  +PMFH
Sbjct: 190 KQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFLPMFH 249

Query: 277 VYGLSIFVMGLLSLGSSIVVMSKFDVKEVVMAIDRFKVTHFPVVPPIMIVMAR--IARKF 336
           V+GL++     L  G+++V M++F+++ V+  I++F+VTH  VVPP+ + +++  I +KF
Sbjct: 250 VFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKF 309

Query: 337 GGHRFRSLKQVSCGAASLSMKIVGDFVQALPHVDFIQGYGMTETTAVGTRGFNTKKAQNH 396
                 SLK +  GAA L   ++ +  + +P+V  +QGYGMTET  + +        +N 
Sbjct: 310 D---LSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRNS 369

Query: 397 LSVGLLAPNMEAKVVDWVSASIMPPGKTGELLLRGPGLMKGYLNNPEATMSTIDQENWLH 456
            S G+LAP +EA++V   +    PP + GE+ +RGP +MKGYLNNP+AT  TID+++W+H
Sbjct: 370 GSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVH 429

Query: 457 TGDIAYFDRDGYLYVVDRLKEVIKYKGFQ------------------------------- 516
           TGD+ YF+ DG LYVVDR+KE+IKYKGFQ                               
Sbjct: 430 TGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGE 489

Query: 517 IPVAFVVKKPGSSLSQKDVIDYVAQQVAPYKKIRKVVFTESIPKSAAGKVLRKELVKHLP 539
           +P+AFVV+ P SS++++D+  ++A+QVAPYK++R+V F   +PKSAAGK+LR+ELV+ + 
Sbjct: 490 VPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVR 544