CmaCh18G013420 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G013420
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioncaffeic acid 3-O-methyltransferase
LocationCma_Chr18: 10209866 .. 10212157 (-)
RNA-Seq ExpressionCmaCh18G013420
SyntenyCmaCh18G013420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTTGAAGTCACTATAAAACCACACTCACCACGGGAATGTATGGGTTGTAATTGCATACCATTCTTTAAGCAAACCAAAGTCCAGGGAGAAAGAAATAGAAAAGAATGGGGTCCTCGGGGAAAGGTTGTGAGGTCCAGATCACCCCCACCACCCGAGTGAGCGATGAAGAAGCACAGCTGTTCGCCATGCAGTTGGCGAGCGCCTCGGTTCTTCCAATGGTGCTGAAGGCCGCCATCGAGCTAGACGTGCTGGAGATCATTGGGGCGGCAGGGGAGGGGGCTTTGTTGTCCCCCTCACAAATTGCCTCTCACCTCTCCTTCTCTGGGGCCAAAACCCCAAACCCTGAGGCGGGTGTGGTTCTAGACCGCATGCTCCGGTTGCTGGCCTCCTACTCCATCCTCACTTACTCCCTCAATACTCTCCCCGATGGCTCACTCCAGAGGCTCTACGGCCTCGCCCCTGTAGCCAAGTTCTTGGTCAAGAATCAAGACGGCGTCTCCATCGCTCCCCTGTGTCTCATGAACCAGGACAAGGTCCTCATGGAGAGCTGGTAATTACCCACCTTTCCATCCTTCCTCTGCTTCAGATTCAAATTACAAATAATAAATCTAACCAAGTGTATGCATAATTAATGATTCAAAATTAACTCTTTTTAGACAATTAGATGTGGAAGTCAGATCAGAGCTTAGCTCCTCTGCTACCCTGCTCCAAAATGTTTTGGGAATAATTTTAATAATTTTATTTTTAAGGAACCATGACTTTATTTGTATGTTTTGGGAATGAAGGTACCATTTGAAAGATGCGGTACTGGAAGGAGGAATTCCATTCAACAAAGCATACGGAATGACGGCGTTCGAATATCATGGAACAGATCCGAGATTCAACAGGGTGTTCAACAGGGGAATGTCCGACCATTCCACCATTACCATGAAGAAGATTCTAGAGACCTACAAAGGCTTCGAAGGCCTTAATTCAGTGGTGGATGTGGGCGGTGGGACCGGAGCTGTTCTCAACATGATAGTCTCCAAGTACCCTTCTATTAGGGGCATCAACTTCGACCTCCCTCATGTCATTCAGGACGCCCCTGCCTACCCAGGTAACCTCTGTTTCGGAACTGGGAATTTAATAAATATGCATCCGAATTCTCAATTGCTTCTTCATTGAAATGGCTGCTGCAGGCGTCCAACATGTTGGGGGAGACATGTTTGTGAGCGTGCCAAAGGGGGATGCTATTTTCATGAAGGTAAAGTAACTGAATTTAGAACACATACACCCATACCTATACCGACTCTGTATTGTATGTATGTATTTGGTCTGATGTAATGTGGGGTTTGCAGTGGATCTGTCACGATTGGAGCGACCAGCACTGCCTGAAATTGTTGAAGAATTGCCACGATTCTCTGCCGGAGCATGGGAAGGTGATCGTGGCAGAATGCATTCTTCCGGTTGCACCAGACAGCAGCCTTGCCACCAAGGGAGTGATTCACATCGATGTCATAATGCTGGCTCACAACCCTGGAGGCAAAGAAAGGACCGAGCAGGAGTTCCACGCCTTGGCCAAGGCTGCTGGATTTCAAGGCTTCAAGGTCCACTCTTGTGCTTTCAACACATACATCATGGAATTTCTCAAGACTCTTTAGTTTCAAACTCCACACATAACCTTCCTCCTCTGCCTTTCTGCCTTTTGGTTTTCAAATCAATCAATAACATCTTTTGTGGCCATAAGTTTATTATTTGGGCAAAAGAATCAATTTTACAACGTGGGACGGATAAACCAAAATGAAAAAGAAAAGTATTAGATAGTACATCGAAGATAGCGCGATGAACTCTTGGCCATTGGATGATAAGGCTGCTTACGACATCGCCGCTGTCATGATGACGTCAAAAGCAAGATATTAAAAATAAGGCTAATTTTGAATGGCTGCGATTTGAGAAATTTAAATAAATAAATAGTCCATGATTAGAGTGGACCCCACTTGTACTCTAGGAGTCAATGGTCGTCGTGCAGATTTTCCGCCCAAATCACAATTCAGGTCGCCAATCCTCTCTTTACTAATTCATAACACAAACAGATAAGAAAAGGCACACTCAAATTTTAAAAAAAAAACGAATCTTTTCAGCTAATAATTGCTATTTTTCTTCTTCTTTTTTTAATTGAAATATCCTCGTTCCCCATCATTCCTTGAACAAATATAAACGGCTTTTGAATTCTAAGTGTGTGGGGTGGAAGAAATATCCCTGAATATTACACTAAGTGTGTGGGGTGGAAGAAATATCCCTGAATATTACA

mRNA sequence

AAGTTGAAGTCACTATAAAACCACACTCACCACGGGAATGTATGGGTTGTAATTGCATACCATTCTTTAAGCAAACCAAAGTCCAGGGAGAAAGAAATAGAAAAGAATGGGGTCCTCGGGGAAAGGTTGTGAGGTCCAGATCACCCCCACCACCCGAGTGAGCGATGAAGAAGCACAGCTGTTCGCCATGCAGTTGGCGAGCGCCTCGGTTCTTCCAATGGTGCTGAAGGCCGCCATCGAGCTAGACGTGCTGGAGATCATTGGGGCGGCAGGGGAGGGGGCTTTGTTGTCCCCCTCACAAATTGCCTCTCACCTCTCCTTCTCTGGGGCCAAAACCCCAAACCCTGAGGCGGGTGTGGTTCTAGACCGCATGCTCCGGTTGCTGGCCTCCTACTCCATCCTCACTTACTCCCTCAATACTCTCCCCGATGGCTCACTCCAGAGGCTCTACGGCCTCGCCCCTGTAGCCAAGTTCTTGGTCAAGAATCAAGACGGCGTCTCCATCGCTCCCCTGTGTCTCATGAACCAGGACAAGGTCCTCATGGAGAGCTGGTACCATTTGAAAGATGCGGTACTGGAAGGAGGAATTCCATTCAACAAAGCATACGGAATGACGGCGTTCGAATATCATGGAACAGATCCGAGATTCAACAGGGTGTTCAACAGGGGAATGTCCGACCATTCCACCATTACCATGAAGAAGATTCTAGAGACCTACAAAGGCTTCGAAGGCCTTAATTCAGTGGTGGATGTGGGCGGTGGGACCGGAGCTGTTCTCAACATGATAGTCTCCAAGTACCCTTCTATTAGGGGCATCAACTTCGACCTCCCTCATGTCATTCAGGACGCCCCTGCCTACCCAGGCGTCCAACATGTTGGGGGAGACATGTTTGTGAGCGTGCCAAAGGGGGATGCTATTTTCATGAAGTGGATCTGTCACGATTGGAGCGACCAGCACTGCCTGAAATTGTTGAAGAATTGCCACGATTCTCTGCCGGAGCATGGGAAGGTGATCGTGGCAGAATGCATTCTTCCGGTTGCACCAGACAGCAGCCTTGCCACCAAGGGAGTGATTCACATCGATGTCATAATGCTGGCTCACAACCCTGGAGGCAAAGAAAGGACCGAGCAGGAGTTCCACGCCTTGGCCAAGGCTGCTGGATTTCAAGGCTTCAAGGTCCACTCTTGTGCTTTCAACACATACATCATGGAATTTCTCAAGACTCTTTAGTTTCAAACTCCACACATAACCTTCCTCCTCTGCCTTTCTGCCTTTTGGTTTTCAAATCAATCAATAACATCTTTTGTGGCCATAAGTTTATTATTTGGGCAAAAGAATCAATTTTACAACGTGGGACGGATAAACCAAAATGAAAAAGAAAAGTATTAGATAGTACATCGAAGATAGCGCGATGAACTCTTGGCCATTGGATGATAAGGCTGCTTACGACATCGCCGCTGTCATGATGACGTCAAAAGCAAGATATTAAAAATAAGGCTAATTTTGAATGGCTGCGATTTGAGAAATTTAAATAAATAAATAGTCCATGATTAGAGTGGACCCCACTTGTACTCTAGGAGTCAATGGTCGTCGTGCAGATTTTCCGCCCAAATCACAATTCAGGTCGCCAATCCTCTCTTTACTAATTCATAACACAAACAGATAAGAAAAGGCACACTCAAATTTTAAAAAAAAAACGAATCTTTTCAGCTAATAATTGCTATTTTTCTTCTTCTTTTTTTAATTGAAATATCCTCGTTCCCCATCATTCCTTGAACAAATATAAACGGCTTTTGAATTCTAAGTGTGTGGGGTGGAAGAAATATCCCTGAATATTACACTAAGTGTGTGGGGTGGAAGAAATATCCCTGAATATTACA

Coding sequence (CDS)

ATGGGGTCCTCGGGGAAAGGTTGTGAGGTCCAGATCACCCCCACCACCCGAGTGAGCGATGAAGAAGCACAGCTGTTCGCCATGCAGTTGGCGAGCGCCTCGGTTCTTCCAATGGTGCTGAAGGCCGCCATCGAGCTAGACGTGCTGGAGATCATTGGGGCGGCAGGGGAGGGGGCTTTGTTGTCCCCCTCACAAATTGCCTCTCACCTCTCCTTCTCTGGGGCCAAAACCCCAAACCCTGAGGCGGGTGTGGTTCTAGACCGCATGCTCCGGTTGCTGGCCTCCTACTCCATCCTCACTTACTCCCTCAATACTCTCCCCGATGGCTCACTCCAGAGGCTCTACGGCCTCGCCCCTGTAGCCAAGTTCTTGGTCAAGAATCAAGACGGCGTCTCCATCGCTCCCCTGTGTCTCATGAACCAGGACAAGGTCCTCATGGAGAGCTGGTACCATTTGAAAGATGCGGTACTGGAAGGAGGAATTCCATTCAACAAAGCATACGGAATGACGGCGTTCGAATATCATGGAACAGATCCGAGATTCAACAGGGTGTTCAACAGGGGAATGTCCGACCATTCCACCATTACCATGAAGAAGATTCTAGAGACCTACAAAGGCTTCGAAGGCCTTAATTCAGTGGTGGATGTGGGCGGTGGGACCGGAGCTGTTCTCAACATGATAGTCTCCAAGTACCCTTCTATTAGGGGCATCAACTTCGACCTCCCTCATGTCATTCAGGACGCCCCTGCCTACCCAGGCGTCCAACATGTTGGGGGAGACATGTTTGTGAGCGTGCCAAAGGGGGATGCTATTTTCATGAAGTGGATCTGTCACGATTGGAGCGACCAGCACTGCCTGAAATTGTTGAAGAATTGCCACGATTCTCTGCCGGAGCATGGGAAGGTGATCGTGGCAGAATGCATTCTTCCGGTTGCACCAGACAGCAGCCTTGCCACCAAGGGAGTGATTCACATCGATGTCATAATGCTGGCTCACAACCCTGGAGGCAAAGAAAGGACCGAGCAGGAGTTCCACGCCTTGGCCAAGGCTGCTGGATTTCAAGGCTTCAAGGTCCACTCTTGTGCTTTCAACACATACATCATGGAATTTCTCAAGACTCTTTAG

Protein sequence

MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGALLSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPVAKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHGKVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHSCAFNTYIMEFLKTL
Homology
BLAST of CmaCh18G013420 vs. ExPASy Swiss-Prot
Match: Q43609 (Caffeic acid 3-O-methyltransferase OS=Prunus dulcis OX=3755 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 5.4e-177
Identity = 307/372 (82.53%), Postives = 334/372 (89.78%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGS+G   E Q+TP T+VSDEEA LFAMQLASASVLPMVLKAAIELD+LEI+  AG G  
Sbjct: 1   MGSTG---ETQMTP-TQVSDEEANLFAMQLASASVLPMVLKAAIELDLLEIMAKAGPGVF 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LSP+ IAS L      T NP+A V+LDRMLRLLASYSILTYSL TL DG ++RLYGL PV
Sbjct: 61  LSPTDIASQL-----PTKNPDAPVMLDRMLRLLASYSILTYSLRTLADGKVERLYGLGPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
            KFL KN++GVSIAPLCLMNQDKVL+ESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 CKFLTKNEEGVSIAPLCLMNQDKVLLESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FN+VFNRGM+DHSTITMKKILETYKGFEGL SVVDVGGGTGAVLNMIVSKYPSI+GINFD
Sbjct: 181 FNKVFNRGMADHSTITMKKILETYKGFEGLTSVVDVGGGTGAVLNMIVSKYPSIKGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVI+DAP YPGV+HVGGDMFVSVPKGDAIFMKWICHDWSD+HCLK LKNC+ +LP++G
Sbjct: 241 LPHVIEDAPQYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPDNG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVI+ ECILPVAPDSSLATKGV+HIDVIMLAHNPGGKERTEQEF ALAK AGFQGF V  
Sbjct: 301 KVILGECILPVAPDSSLATKGVVHIDVIMLAHNPGGKERTEQEFQALAKGAGFQGFNVAC 360

Query: 361 CAFNTYIMEFLK 373
            AFNTY++EFLK
Sbjct: 361 SAFNTYVIEFLK 363

BLAST of CmaCh18G013420 vs. ExPASy Swiss-Prot
Match: Q8GU25 (Caffeic acid 3-O-methyltransferase OS=Rosa chinensis OX=74649 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 2.3e-175
Identity = 301/374 (80.48%), Postives = 336/374 (89.84%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGS+G   E Q+TP T+VSDEEA LFAMQLASASVLPMVLKAAIELD+LEI+  AG GA 
Sbjct: 1   MGSTG---ETQMTP-TQVSDEEANLFAMQLASASVLPMVLKAAIELDLLEIMAKAGPGAF 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LSP+ +AS L      T NPEA V+LDRMLRLLASYSILTYSL TLPDG ++RLYGL PV
Sbjct: 61  LSPNDLASQL-----PTKNPEAPVMLDRMLRLLASYSILTYSLRTLPDGKVERLYGLGPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
            KFL KN+DGVSIA LCLMNQDKVL+ESWYHLKDAVL+GGIPFNKAYGMTAF+YHGTDPR
Sbjct: 121 CKFLTKNEDGVSIAALCLMNQDKVLVESWYHLKDAVLDGGIPFNKAYGMTAFDYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FN+VFN+GM+DHSTITMKKILETYKGFEGL S+VDVGGGTGAV+NMIVSKYPSI+GINFD
Sbjct: 181 FNKVFNKGMADHSTITMKKILETYKGFEGLTSIVDVGGGTGAVVNMIVSKYPSIKGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVI+DAP YPGVQHVGGDMFVSVPKGDAIFMKWICHDWSD+HCLK LKNC+ +LP++G
Sbjct: 241 LPHVIEDAPQYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALPDNG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVI+ ECILPVAPD+SLATKGV+HIDV+MLAHNPGGKERTEQEF ALAK +GFQG +V  
Sbjct: 301 KVILGECILPVAPDTSLATKGVVHIDVVMLAHNPGGKERTEQEFEALAKGSGFQGIRVAC 360

Query: 361 CAFNTYIMEFLKTL 375
            AFNTY++EFLK +
Sbjct: 361 NAFNTYVIEFLKKI 365

BLAST of CmaCh18G013420 vs. ExPASy Swiss-Prot
Match: Q00763 (Caffeic acid 3-O-methyltransferase 1 OS=Populus tremuloides OX=3693 GN=OMT1 PE=1 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 2.1e-173
Identity = 295/372 (79.30%), Postives = 334/372 (89.78%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGS+G   E Q+TP T+VSDEEA LFAMQLASASVLPM+LK AIELD+LEI+  AG GA 
Sbjct: 1   MGSTG---ETQMTP-TQVSDEEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAF 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LS S+IASHL      T NP+A V+LDR+LRLLASYSILT SL  LPDG ++RLYGLAPV
Sbjct: 61  LSTSEIASHL-----PTKNPDAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGLAPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
            KFL KN+DGVS++PLCLMNQDKVLMESWY+LKDA+L+GGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 CKFLTKNEDGVSVSPLCLMNQDKVLMESWYYLKDAILDGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FN+VFN+GMSDHSTITMKKILETYKGFEGL S+VDVGGGTGAV+N IVSKYPSI+GINFD
Sbjct: 181 FNKVFNKGMSDHSTITMKKILETYKGFEGLTSLVDVGGGTGAVVNTIVSKYPSIKGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVI+DAP+YPGV+HVGGDMFVSVPK DA+FMKWICHDWSD HCLK LKNC+D+LPE+G
Sbjct: 241 LPHVIEDAPSYPGVEHVGGDMFVSVPKADAVFMKWICHDWSDAHCLKFLKNCYDALPENG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVI+ ECILPVAPD+SLATKGV+H+DVIMLAHNPGGKERTE+EF  LAK AGFQGF+V  
Sbjct: 301 KVILVECILPVAPDTSLATKGVVHVDVIMLAHNPGGKERTEKEFEGLAKGAGFQGFEVMC 360

Query: 361 CAFNTYIMEFLK 373
           CAFNT+++EF K
Sbjct: 361 CAFNTHVIEFRK 363

BLAST of CmaCh18G013420 vs. ExPASy Swiss-Prot
Match: Q43046 (Caffeic acid 3-O-methyltransferase 1 OS=Populus kitakamiensis OX=34292 GN=HOMT1 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 1.4e-172
Identity = 294/372 (79.03%), Postives = 332/372 (89.25%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGS+G   E Q+TP T+VSDEEA LFAMQLASASVLPM+LK AIELD+LEI+  AG GA 
Sbjct: 1   MGSTG---ETQMTP-TQVSDEEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAF 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LS S+IASHL      T NP+A V+LDR+LRLLASYSILT SL  LPDG ++RLYGLAPV
Sbjct: 61  LSTSEIASHL-----PTKNPDAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGLAPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
            KFL KN+DGVS++PLCLMNQDKVLMESWY+LKDA+LEGGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 CKFLTKNEDGVSVSPLCLMNQDKVLMESWYYLKDAILEGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FN+VFN+GMSDHSTITMKKILETYKGFEGL S+VDVGGGTGAV+N IVSKYPSI+GINFD
Sbjct: 181 FNKVFNKGMSDHSTITMKKILETYKGFEGLTSLVDVGGGTGAVVNTIVSKYPSIKGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVI+DAP+YPGV+HVGGDMFVSVP  DA+FMKWICHDWSD HCLK LKNC+D+LPE+G
Sbjct: 241 LPHVIEDAPSYPGVEHVGGDMFVSVPNADAVFMKWICHDWSDAHCLKFLKNCYDALPENG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVI+ ECILPVAPD+SLATKGV+H+DVIMLAHNPGGKERTE+EF  LA  AGFQGF+V  
Sbjct: 301 KVILVECILPVAPDTSLATKGVVHVDVIMLAHNPGGKERTEKEFEGLANGAGFQGFEVMC 360

Query: 361 CAFNTYIMEFLK 373
           CAFNT+++EF K
Sbjct: 361 CAFNTHVIEFRK 363

BLAST of CmaCh18G013420 vs. ExPASy Swiss-Prot
Match: O23760 (Caffeic acid 3-O-methyltransferase OS=Clarkia breweri OX=36903 GN=COMT PE=1 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 3.1e-172
Identity = 297/376 (78.99%), Postives = 333/376 (88.56%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAA---GE 60
           MGS+G   E Q+TP T VSDEEA LFAMQLASASVLPMVLKAAIELDVLEI+  +   G 
Sbjct: 1   MGSTG-NAETQLTP-THVSDEEANLFAMQLASASVLPMVLKAAIELDVLEIMAKSIPHGS 60

Query: 61  GALLSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGL 120
           GA +SP++IA+ L      T NP+A V+LDR+LRLLASYS++T SL  LPDG ++RLYGL
Sbjct: 61  GAYISPAEIAAQL-----PTTNPDAPVMLDRVLRLLASYSVVTCSLRELPDGKVERLYGL 120

Query: 121 APVAKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGT 180
           APV KFL KN+DGVS+APLCLMNQDKVLMESWY+LKDA+L+GGIPFNKAYGMTAFEYHGT
Sbjct: 121 APVCKFLTKNEDGVSLAPLCLMNQDKVLMESWYYLKDAILDGGIPFNKAYGMTAFEYHGT 180

Query: 181 DPRFNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGI 240
           DPRFN+VFNRGMSDHSTITMKKI E Y GFE LN++VDVGGGTGAVL+MIV+KYPSI+GI
Sbjct: 181 DPRFNKVFNRGMSDHSTITMKKIFEMYTGFEALNTIVDVGGGTGAVLSMIVAKYPSIKGI 240

Query: 241 NFDLPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLP 300
           NFDLPHVI+DAP YPGV+HVGGDMFVSVPKGDAIFMKWICHDWSD+HCLK LKNC+ +LP
Sbjct: 241 NFDLPHVIEDAPIYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYAALP 300

Query: 301 EHGKVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFK 360
           EHGKVIVAECILP++PD SLATKGVIHID IMLAHNPGGKERTE+EF ALA  AGF+GFK
Sbjct: 301 EHGKVIVAECILPLSPDPSLATKGVIHIDAIMLAHNPGGKERTEKEFEALAIGAGFKGFK 360

Query: 361 VHSCAFNTYIMEFLKT 374
           V  CAFNTY+MEFLKT
Sbjct: 361 VACCAFNTYVMEFLKT 369

BLAST of CmaCh18G013420 vs. ExPASy TrEMBL
Match: A0A6J1HX11 (caffeic acid 3-O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111467633 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 1.8e-215
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL
Sbjct: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV
Sbjct: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
           AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD
Sbjct: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG
Sbjct: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS
Sbjct: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360

Query: 361 CAFNTYIMEFLKTL 375
           CAFNTYIMEFLKTL
Sbjct: 361 CAFNTYIMEFLKTL 374

BLAST of CmaCh18G013420 vs. ExPASy TrEMBL
Match: A0A6J1G2C2 (caffeic acid 3-O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111450048 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.4e-212
Identity = 369/374 (98.66%), Postives = 371/374 (99.20%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGSSG+GCEVQITPTT+VSDEEAQLFAMQLASASVLPMVLKAAIELDVLEII AAGEGAL
Sbjct: 1   MGSSGQGCEVQITPTTQVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIAAAGEGAL 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV
Sbjct: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
           AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD
Sbjct: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG
Sbjct: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVIVAECILP APDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHAL KAAGFQGFKVHS
Sbjct: 301 KVIVAECILPDAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALGKAAGFQGFKVHS 360

Query: 361 CAFNTYIMEFLKTL 375
           CAFNTYIMEFLKTL
Sbjct: 361 CAFNTYIMEFLKTL 374

BLAST of CmaCh18G013420 vs. ExPASy TrEMBL
Match: A0A5D3CWE6 (Caffeic acid 3-O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold692G00830 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.8e-182
Identity = 318/354 (89.83%), Postives = 336/354 (94.92%), Query Frame = 0

Query: 20  DEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGALLSPSQIASHLSFSGAKTPN 79
           +EEAQLFAMQLASASVLPMVLK AIELD+LEIIG  GEGALLSPSQIAS L  SG K  N
Sbjct: 97  EEEAQLFAMQLASASVLPMVLKTAIELDLLEIIGRGGEGALLSPSQIASQL--SGLK--N 156

Query: 80  PEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPVAKFLVKNQDGVSIAPLCLM 139
           PEA V+LDRMLRLLASYSILT SLN LPDGSLQRLYGLAPV+KFL+KNQDGVSIAPLCLM
Sbjct: 157 PEAHVMLDRMLRLLASYSILTCSLNPLPDGSLQRLYGLAPVSKFLIKNQDGVSIAPLCLM 216

Query: 140 NQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNRVFNRGMSDHSTITMKK 199
           NQDKVLMESWYHLKDAVLEGGIPFN+AYG++AFEYHGTDPRFN+VFN+GMSDHSTITMKK
Sbjct: 217 NQDKVLMESWYHLKDAVLEGGIPFNRAYGISAFEYHGTDPRFNKVFNKGMSDHSTITMKK 276

Query: 200 ILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPAYPGVQHVGG 259
           ILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAP YPGV+HVGG
Sbjct: 277 ILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPPYPGVEHVGG 336

Query: 260 DMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHGKVIVAECILPVAPDSSLAT 319
           DMFVSVPKGDAIFMKWICHDWSD HCLK LKNC+D+LPEHGKVIVAECILPVAPD+SLAT
Sbjct: 337 DMFVSVPKGDAIFMKWICHDWSDHHCLKFLKNCYDALPEHGKVIVAECILPVAPDASLAT 396

Query: 320 KGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHSCAFNTYIMEFLKT 374
           KGVIHID+IMLAHNPGGKERTE+EF AL+KAAGF GFKVH CAFNTY+MEFLKT
Sbjct: 397 KGVIHIDLIMLAHNPGGKERTEKEFQALSKAAGFLGFKVHCCAFNTYVMEFLKT 446

BLAST of CmaCh18G013420 vs. ExPASy TrEMBL
Match: A0A1S3CIH3 (caffeic acid 3-O-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103501180 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.8e-182
Identity = 318/354 (89.83%), Postives = 336/354 (94.92%), Query Frame = 0

Query: 20  DEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGALLSPSQIASHLSFSGAKTPN 79
           +EEAQLFAMQLASASVLPMVLK AIELD+LEIIG  GEGALLSPSQIAS L  SG K  N
Sbjct: 9   EEEAQLFAMQLASASVLPMVLKTAIELDLLEIIGRGGEGALLSPSQIASQL--SGLK--N 68

Query: 80  PEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPVAKFLVKNQDGVSIAPLCLM 139
           PEA V+LDRMLRLLASYSILT SLN LPDGSLQRLYGLAPV+KFL+KNQDGVSIAPLCLM
Sbjct: 69  PEAHVMLDRMLRLLASYSILTCSLNPLPDGSLQRLYGLAPVSKFLIKNQDGVSIAPLCLM 128

Query: 140 NQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNRVFNRGMSDHSTITMKK 199
           NQDKVLMESWYHLKDAVLEGGIPFN+AYG++AFEYHGTDPRFN+VFN+GMSDHSTITMKK
Sbjct: 129 NQDKVLMESWYHLKDAVLEGGIPFNRAYGISAFEYHGTDPRFNKVFNKGMSDHSTITMKK 188

Query: 200 ILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPAYPGVQHVGG 259
           ILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAP YPGV+HVGG
Sbjct: 189 ILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPPYPGVEHVGG 248

Query: 260 DMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHGKVIVAECILPVAPDSSLAT 319
           DMFVSVPKGDAIFMKWICHDWSD HCLK LKNC+D+LPEHGKVIVAECILPVAPD+SLAT
Sbjct: 249 DMFVSVPKGDAIFMKWICHDWSDHHCLKFLKNCYDALPEHGKVIVAECILPVAPDASLAT 308

Query: 320 KGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHSCAFNTYIMEFLKT 374
           KGVIHID+IMLAHNPGGKERTE+EF AL+KAAGF GFKVH CAFNTY+MEFLKT
Sbjct: 309 KGVIHIDLIMLAHNPGGKERTEKEFQALSKAAGFLGFKVHCCAFNTYVMEFLKT 358

BLAST of CmaCh18G013420 vs. ExPASy TrEMBL
Match: A0A0A0L0G6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G091880 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 8.4e-181
Identity = 315/354 (88.98%), Postives = 335/354 (94.63%), Query Frame = 0

Query: 20  DEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGALLSPSQIASHLSFSGAKTPN 79
           +EEAQLFAMQLASASVLPMVLK AIELD+LEIIG  GEGALLSPSQIAS L  SG K  N
Sbjct: 9   EEEAQLFAMQLASASVLPMVLKTAIELDLLEIIGRGGEGALLSPSQIASQL--SGLK--N 68

Query: 80  PEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPVAKFLVKNQDGVSIAPLCLM 139
           PEA V+LDRMLRLLASYSILT SL+ LPDGS+QRLYGLAPV+KFL+KN DGVSIAPLCLM
Sbjct: 69  PEAHVMLDRMLRLLASYSILTCSLDPLPDGSVQRLYGLAPVSKFLIKNHDGVSIAPLCLM 128

Query: 140 NQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNRVFNRGMSDHSTITMKK 199
           NQDKVLMESWYHLKDAVLEGGIPFN+AYGM+AFEYHGTDPRFN+VFN+GMSDHSTITMKK
Sbjct: 129 NQDKVLMESWYHLKDAVLEGGIPFNRAYGMSAFEYHGTDPRFNKVFNKGMSDHSTITMKK 188

Query: 200 ILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPAYPGVQHVGG 259
           ILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAP YPGV+HVGG
Sbjct: 189 ILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPPYPGVEHVGG 248

Query: 260 DMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHGKVIVAECILPVAPDSSLAT 319
           DMFVSVPKGDAIFMKWICHDWSD HCLK LKNC+D+LPEHGKVIVAECILP+APD+SLAT
Sbjct: 249 DMFVSVPKGDAIFMKWICHDWSDHHCLKFLKNCYDALPEHGKVIVAECILPLAPDASLAT 308

Query: 320 KGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHSCAFNTYIMEFLKT 374
           KGVIHID+IMLAHNPGGKERTE+EF AL+KAAGF GFKVH CAFNTY+MEFLKT
Sbjct: 309 KGVIHIDLIMLAHNPGGKERTEKEFQALSKAAGFDGFKVHCCAFNTYVMEFLKT 358

BLAST of CmaCh18G013420 vs. NCBI nr
Match: XP_022968370.1 (caffeic acid 3-O-methyltransferase [Cucurbita maxima])

HSP 1 Score: 758.1 bits (1956), Expect = 3.7e-215
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL
Sbjct: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV
Sbjct: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
           AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD
Sbjct: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG
Sbjct: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS
Sbjct: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360

Query: 361 CAFNTYIMEFLKTL 375
           CAFNTYIMEFLKTL
Sbjct: 361 CAFNTYIMEFLKTL 374

BLAST of CmaCh18G013420 vs. NCBI nr
Match: KAG7013334.1 (Caffeic acid 3-O-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 752.3 bits (1941), Expect = 2.0e-213
Identity = 370/374 (98.93%), Postives = 373/374 (99.73%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGSSG+GCEVQITPTT+VSDEEAQLFAMQLASASVLPMVLKAAIELDVLEII AAGEGAL
Sbjct: 1   MGSSGQGCEVQITPTTQVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIAAAGEGAL 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV
Sbjct: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
           AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD
Sbjct: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG
Sbjct: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS
Sbjct: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360

Query: 361 CAFNTYIMEFLKTL 375
           CAFNTY+MEFLKTL
Sbjct: 361 CAFNTYVMEFLKTL 374

BLAST of CmaCh18G013420 vs. NCBI nr
Match: KAG6574266.1 (Caffeic acid 3-O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 748.8 bits (1932), Expect = 2.2e-212
Identity = 369/374 (98.66%), Postives = 372/374 (99.47%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGSSG+GCEVQITPTT+VSDEEAQLFAMQLASASVLPMVLKAAIELDVLEII AAGEGAL
Sbjct: 1   MGSSGQGCEVQITPTTQVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIAAAGEGAL 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILT SLNTLPDGSLQRLYGLAPV
Sbjct: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTCSLNTLPDGSLQRLYGLAPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
           AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD
Sbjct: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG
Sbjct: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS
Sbjct: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360

Query: 361 CAFNTYIMEFLKTL 375
           CAFNTY+MEFLKTL
Sbjct: 361 CAFNTYVMEFLKTL 374

BLAST of CmaCh18G013420 vs. NCBI nr
Match: XP_022945961.1 (caffeic acid 3-O-methyltransferase [Cucurbita moschata])

HSP 1 Score: 748.4 bits (1931), Expect = 2.9e-212
Identity = 369/374 (98.66%), Postives = 371/374 (99.20%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGSSG+GCEVQITPTT+VSDEEAQLFAMQLASASVLPMVLKAAIELDVLEII AAGEGAL
Sbjct: 1   MGSSGQGCEVQITPTTQVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIAAAGEGAL 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV
Sbjct: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
           AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD
Sbjct: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG
Sbjct: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVIVAECILP APDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHAL KAAGFQGFKVHS
Sbjct: 301 KVIVAECILPDAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALGKAAGFQGFKVHS 360

Query: 361 CAFNTYIMEFLKTL 375
           CAFNTYIMEFLKTL
Sbjct: 361 CAFNTYIMEFLKTL 374

BLAST of CmaCh18G013420 vs. NCBI nr
Match: XP_023542318.1 (caffeic acid 3-O-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 747.3 bits (1928), Expect = 6.5e-212
Identity = 369/374 (98.66%), Postives = 371/374 (99.20%), Query Frame = 0

Query: 1   MGSSGKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGAL 60
           MGSSG+GCEVQITPTT+VSDEEAQLFAMQLASASVLPMVLKAAIELDVLEII AAGEGAL
Sbjct: 1   MGSSGQGCEVQITPTTQVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIAAAGEGAL 60

Query: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120
           LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV
Sbjct: 61  LSPSQIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPV 120

Query: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPR 180
           AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKD VLEGGIPFNKAYGMTAFEYHGTDPR
Sbjct: 121 AKFLVKNQDGVSIAPLCLMNQDKVLMESWYHLKDTVLEGGIPFNKAYGMTAFEYHGTDPR 180

Query: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240
           FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD
Sbjct: 181 FNRVFNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFD 240

Query: 241 LPHVIQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300
           LPHVIQDA AYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG
Sbjct: 241 LPHVIQDALAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHG 300

Query: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360
           KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS
Sbjct: 301 KVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHS 360

Query: 361 CAFNTYIMEFLKTL 375
           CAFNTYIMEFLKTL
Sbjct: 361 CAFNTYIMEFLKTL 374

BLAST of CmaCh18G013420 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 548.9 bits (1413), Expect = 3.2e-156
Identity = 267/370 (72.16%), Postives = 314/370 (84.86%), Query Frame = 0

Query: 5   GKGCEVQITPTTRVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGALLSPS 64
           G   E Q+TP  +V+D+EA LFAMQLASASVLPM LK+A+ELD+LEI+  A  G+ +SP+
Sbjct: 2   GSTAETQLTP-VQVTDDEAALFAMQLASASVLPMALKSALELDLLEIM--AKNGSPMSPT 61

Query: 65  QIASHLSFSGAKTPNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPVAKFL 124
           +IAS L      T NPEA V+LDR+LRLL SYS+LT S   L    ++R+YGL PV K+L
Sbjct: 62  EIASKL-----PTKNPEAPVMLDRILRLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYL 121

Query: 125 VKNQDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNRV 184
            KN+DGVSIA LCLMNQDKVLMESWYHLKDA+L+GGIPFNKAYGM+AFEYHGTDPRFN+V
Sbjct: 122 TKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMSAFEYHGTDPRFNKV 181

Query: 185 FNRGMSDHSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHV 244
           FN GMS+HSTITMKKILETYKGFEGL S+VDVGGG GA L MIVSKYP+++GINFDLPHV
Sbjct: 182 FNNGMSNHSTITMKKILETYKGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHV 241

Query: 245 IQDAPAYPGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHGKVIV 304
           I+DAP++PG++HVGGDMFVSVPKGDAIFMKWICHDWSD+HC+K LKNC++SLPE GKVI+
Sbjct: 242 IEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVIL 301

Query: 305 AECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHSCAFN 364
           AECILP  PDSSL+TK V+H+D IMLAHNPGGKERTE+EF ALAKA+GF+G KV   AF 
Sbjct: 302 AECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFG 361

Query: 365 TYIMEFLKTL 375
             ++E LK L
Sbjct: 362 VNLIELLKKL 363

BLAST of CmaCh18G013420 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 334.3 bits (856), Expect = 1.2e-91
Identity = 170/361 (47.09%), Postives = 241/361 (66.76%), Query Frame = 0

Query: 18  VSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGALLSPSQIASHLSFSGAKT 77
           + ++   L A  + +A   PMVLKAA+EL V++ I AA  G  LSPS+IA  L     K 
Sbjct: 24  IDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLP---NKP 83

Query: 78  PNPEAGVVLDRMLRLLASYSIL----TYSLNTLPDGSLQRLYGLAPVAKFLVKNQDGV-S 137
            NPEA V+LDRMLRLL S+SIL      S      G ++R+Y   P+ K+ +K+ DG  S
Sbjct: 84  TNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGS 143

Query: 138 IAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNRVFNRGMSDH 197
           ++ L L+   +V++++W +LKD +LEG   F+ A+ M  FEY  +D +F+++F+R MS+ 
Sbjct: 144 LSSLLLLLHSQVILKTWTNLKDVILEGKDAFSSAHDMRLFEYISSDDQFSKLFHRAMSES 203

Query: 198 STITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPAYP 257
           ST+ MKK+LE Y+GFE +N++VDVGGG G +L +I SKYP I+G+NFDL  V+  AP YP
Sbjct: 204 STMVMKKVLEEYRGFEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQAPFYP 263

Query: 258 GVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHGKVIVAECILPVA 317
           GV+HV GDMF+ VPKGDAIFMKWI HDW D+ C+K+LKNC  SLPE GKVI+ E I P+ 
Sbjct: 264 GVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEKGKVIIVEMITPME 323

Query: 318 P-DSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHSCAFNTYIMEFL 373
           P  +  +   V+ +D++ML    GGKER+  +F  LA A+GF   ++   +++  ++EF 
Sbjct: 324 PKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYSVIEFH 381

BLAST of CmaCh18G013420 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.9e-91
Identity = 169/361 (46.81%), Postives = 233/361 (64.54%), Query Frame = 0

Query: 18  VSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGALLSPSQIASHLSFSGAKT 77
           V ++   L A  + +    PMVLKAA EL V++ I AAG    LSP +IA  L     K 
Sbjct: 24  VDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLP---TKP 83

Query: 78  PNPEAGVVLDRMLRLLASYSILTYSLNTLPD----GSLQRLYGLAPVAKFLVKNQDGV-S 137
            NPEA V+LDRML LL S+SIL   +    +    G ++R+Y   PV K+ +++ DG  S
Sbjct: 84  TNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGS 143

Query: 138 IAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNRVFNRGMSDH 197
           + PL ++   +V  ++W +LKD +LEG   FN A+GM  FEY  +D  F  +FNR MS+ 
Sbjct: 144 LVPLFMLLHTQVFFKTWTNLKDVILEGRDAFNSAHGMKIFEYINSDQPFAELFNRAMSEP 203

Query: 198 STITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPAYP 257
           ST+ MKK+L+ Y+GFE +N++VDVGGG G VL ++ SKYP I+G+NFDL  V+  AP YP
Sbjct: 204 STMIMKKVLDVYRGFEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYP 263

Query: 258 GVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHGKVIVAECILPVA 317
           GV+HV GDMFV VPKGDA+FMKWI HDW D+ C+K+LKNC  SLPE GK+I+ E + P  
Sbjct: 264 GVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEKGKIIIVEFVTPKE 323

Query: 318 P-DSSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHSCAFNTYIMEFL 373
           P    L++  V  +D++ML    GGKER+  +F  LA A+GF   ++   A++  ++EF 
Sbjct: 324 PKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIEFH 381

BLAST of CmaCh18G013420 vs. TAIR 10
Match: AT1G33030.1 (O-methyltransferase family protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.2e-86
Identity = 163/357 (45.66%), Postives = 236/357 (66.11%), Query Frame = 0

Query: 18  VSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGALLSPSQIASHLSFSGAKT 77
           + ++    +AM L+S+SVLPMVLK AI+L + +I+  +G     S SQI S LS    +T
Sbjct: 1   MEEQNLSSYAMILSSSSVLPMVLKTAIDLGLFDILAESGPS---SASQIFSLLS---NET 60

Query: 78  PNPEAGVVLDRMLRLLASYSILTYSLNTLPDGSLQRLYGLAPVAKFLVKNQD-GVSIAPL 137
                  +++R+LR LASYSILT S++T   G    +YGLAPVAK+  KNQ+ G S+AP+
Sbjct: 61  KKHHDSSLVNRILRFLASYSILTCSVST-EHGEPFAIYGLAPVAKYFTKNQNGGGSLAPM 120

Query: 138 CLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNRVFNRGMSDHSTIT 197
             + QDKV+ + WY+LKD+VLEGG+PFN  +G +A E  G+D RF  VF   M   + + 
Sbjct: 121 VNLFQDKVVTDMWYNLKDSVLEGGLPFNNTHGSSAVELVGSDSRFREVFQSSMKGFNEVF 180

Query: 198 MKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPS-IRGINFDLPHVIQDAPAYPGVQ 257
           +++ L+ Y GF+G+ S+VDVGGG G++L+ I+SK+   I+ INFDLP VI  +   PG++
Sbjct: 181 IEEFLKNYNGFDGVKSLVDVGGGDGSLLSRIISKHTHIIKAINFDLPTVINTSLPSPGIE 240

Query: 258 HVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHGKVIVAECILPVAPDS 317
           HV GDMF + PKG+AIFMKW+ H W D HC+K+L NC+ SLP +GKVIV + ++P  P  
Sbjct: 241 HVAGDMFTNTPKGEAIFMKWMLHSWDDDHCVKILSNCYQSLPSNGKVIVVDMVIPEFPGD 300

Query: 318 SLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHSCAFNTYIMEFLK 373
           +L  + +   ++ M+  NP GKERT++EF  LA+ AGF   +V   +    ++EF K
Sbjct: 301 TLLDRSLFQFELFMMNMNPSGKERTKKEFEILARLAGFSNVQVPFTSLCFSVLEFHK 350

BLAST of CmaCh18G013420 vs. TAIR 10
Match: AT1G63140.2 (O-methyltransferase family protein )

HSP 1 Score: 315.1 bits (806), Expect = 7.7e-86
Identity = 167/362 (46.13%), Postives = 232/362 (64.09%), Query Frame = 0

Query: 17  RVSDEEAQLFAMQLASASVLPMVLKAAIELDVLEIIGAAGEGALLSPSQIASHLSFSGAK 76
           R  ++   L A ++  A   PMVLK A+EL V+++I +  +G  LSPS+IA  L     K
Sbjct: 23  RYHEDTVSLQAERILHAMTFPMVLKTALELGVIDMITSVDDGVWLSPSEIALGLP---TK 82

Query: 77  TPNPEAGVVLDRMLRLLASYSILTYSL----NTLPDGSLQRLYGLAPVAKFLVKNQDGV- 136
             NPEA V+LDRML LLAS+SIL Y      + +     +R+Y   PV  F +   DG+ 
Sbjct: 83  PTNPEAPVLLDRMLVLLASHSILKYRTVETGDNIGSRKTERVYAAEPVCTFFLNRGDGLG 142

Query: 137 SIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNRVFNRGMSD 196
           S+A L ++ Q +V M+ W HLKD +LEG   F  A+GM  FE  G++ +F  +FNR MS+
Sbjct: 143 SLATLFMVLQGEVCMKPWEHLKDMILEGKDAFTSAHGMRFFELIGSNEQFAEMFNRAMSE 202

Query: 197 HSTITMKKILETYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPAY 256
            ST+ MKK+LE YKGFE +N++VDVGGG G ++  + SKYP I+GINFDL  V+  AP  
Sbjct: 203 ASTLIMKKVLEVYKGFEDVNTLVDVGGGIGTIIGQVTSKYPHIKGINFDLASVLAHAPFN 262

Query: 257 PGVQHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKLLKNCHDSLPEHGKVIVAECILPV 316
            GV+HV GDMF  +PKGDAIFMKWI HDW+D+ C+K+LKN   SLPE GKVI+ E + P 
Sbjct: 263 KGVEHVSGDMFKEIPKGDAIFMKWILHDWTDEDCVKILKNYWKSLPEKGKVIIVEVVTPE 322

Query: 317 APD-SSLATKGVIHIDVIMLAHNPGGKERTEQEFHALAKAAGFQGFKVHSCAFNTYIMEF 373
            P  + +++  V  +D++MLA + GGKER+  +F  LA  +GF   ++   AF+  ++E 
Sbjct: 323 EPKINDISSNIVFGMDMLMLAVSSGGKERSLSQFETLASDSGFLRCEIICHAFSYSVIEL 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q436095.4e-17782.53Caffeic acid 3-O-methyltransferase OS=Prunus dulcis OX=3755 GN=COMT1 PE=2 SV=1[more]
Q8GU252.3e-17580.48Caffeic acid 3-O-methyltransferase OS=Rosa chinensis OX=74649 GN=COMT1 PE=2 SV=1[more]
Q007632.1e-17379.30Caffeic acid 3-O-methyltransferase 1 OS=Populus tremuloides OX=3693 GN=OMT1 PE=1... [more]
Q430461.4e-17279.03Caffeic acid 3-O-methyltransferase 1 OS=Populus kitakamiensis OX=34292 GN=HOMT1 ... [more]
O237603.1e-17278.99Caffeic acid 3-O-methyltransferase OS=Clarkia breweri OX=36903 GN=COMT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HX111.8e-215100.00caffeic acid 3-O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111467633 P... [more]
A0A6J1G2C21.4e-21298.66caffeic acid 3-O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111450048... [more]
A0A5D3CWE65.8e-18289.83Caffeic acid 3-O-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CIH35.8e-18289.83caffeic acid 3-O-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103501180 PE=... [more]
A0A0A0L0G68.4e-18188.98Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G091880 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022968370.13.7e-215100.00caffeic acid 3-O-methyltransferase [Cucurbita maxima][more]
KAG7013334.12.0e-21398.93Caffeic acid 3-O-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6574266.12.2e-21298.66Caffeic acid 3-O-methyltransferase, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022945961.12.9e-21298.66caffeic acid 3-O-methyltransferase [Cucurbita moschata][more]
XP_023542318.16.5e-21298.66caffeic acid 3-O-methyltransferase [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G54160.13.2e-15672.16O-methyltransferase 1 [more]
AT1G77530.11.2e-9147.09O-methyltransferase family protein [more]
AT1G77520.17.9e-9146.81O-methyltransferase family protein [more]
AT1G33030.11.2e-8645.66O-methyltransferase family protein [more]
AT1G63140.27.7e-8646.13O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 149..354
e-value: 1.5E-79
score: 266.2
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 38..94
e-value: 2.5E-18
score: 65.8
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 7..124
e-value: 2.4E-40
score: 138.6
IPR016461O-methyltransferase COMT-typePIRSFPIRSF005739O-mtasecoord: 7..372
e-value: 1.3E-141
score: 469.6
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 6..372
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 28..372
score: 74.649902
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 125..372
e-value: 2.1E-98
score: 330.2
NoneNo IPR availablePIRSRPIRSR005739-1PIRSR005739-1coord: 37..370
e-value: 3.6E-100
score: 332.6
NoneNo IPR availablePANTHERPTHR11746:SF260BNAA10G07270D PROTEINcoord: 6..372
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 213..304
e-value: 2.60341E-4
score: 38.1799
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 19..127
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 129..370

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G013420.1CmaCh18G013420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051555 flavonol biosynthetic process
biological_process GO:0009809 lignin biosynthetic process
biological_process GO:0030187 melatonin biosynthetic process
biological_process GO:0032259 methylation
molecular_function GO:0017096 acetylserotonin O-methyltransferase activity
molecular_function GO:0047763 caffeate O-methyltransferase activity
molecular_function GO:0030744 luteolin O-methyltransferase activity
molecular_function GO:0033799 myricetin 3'-O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0030755 quercetin 3-O-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0008171 O-methyltransferase activity