CmaCh18G011580 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh18G011580
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	sphinganine C4-monooxygenase 2
Location	Cma_Chr18: 9281681 .. 9284685 (-)
RNA-Seq Expression	CmaCh18G011580
Synteny	CmaCh18G011580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTTGTATTCTCTGATCAAATGTTGGGTACTTTTGTGCCAATTGTGGTTTACTGGTTGTATTCTGGGATTTATATTCTACTGGGCTCATTTGAGAACTACCGGTTGCACTCCAAGGAAGATGAGCTAGAGAAGAACGTAGTGTCAAAGAGCACTGTGGTCAGAGGCGTTTTGCTTCAACAGACTATTCAGGCAGTCGTTGCGATCCTCTTGTTTAAGGTACGCATTGCATCATGTCAGTTCTGAGTTCATTTAATTTCAATCTGTATATGATTTTGATTATGTTTTGTGAATTTGTCATGTCCCCTGTATAAAGTTTGAATCAATTGACTGTTATTAGTTGAAAGAGGCGGCGCTGAGTCCACTTTGATGCGAATTTATCGCTGTATTTTGTTAACTCCCCCCCGGTCCCCCACTTGCTTTAAGTTATGGATTTGTAGACACCATTTATTGATGCTTTAAGACTGTCACTTCAATTTTTTTGTTGAGAAGTAATCGTACAAGTGCTTAGCTGCGCGAAATTTATTACTGATCTGAAACTTCATCAGTTCTTTTATACTTTACAAGCTGACAGCATTAGTAGGTGGAGCGTTGGACCTTTGTACTTCCTAGTCTTGCTGACCAAGATTGGTTTATGTATTATTTTCTGAGGATTCCTTTTGTTATTTCTGAATCATCTTTTTTTATTTCACCTAGTTAGGTCACTTTCCATGAAAATATTCCATCCATGATCCTCAAACTACTCCACAATTCTTACAAAAAAAAAAAAAGAGTAACCTCCTACTTCGAGGTTCAGGTTTAGGAGAGGTGTGCTTTTAGCTCTATAGTCATGTAGCTCTTTTCACTACACTAATTGTGTAAGTGAAGCAGGTGCTATGTAGGTGTCTGGATATCCCGACATGTTGAGGTTGGTAGTGGGCGGGTCTAAAGGCAAGAGCATGGTCCAGCCATTTATCACCAGTCAACGACCAAGCAAAGAAGTCAAGTAAGATGAGCATTTTCTATTGGAAATGCTCGTCTAACCTGTGGTCTCTTAGGAAACTATATAATCGTAAAAACTCAACCATTGAGTCTTCACCAAATTTGGTACTTAGGATGTGCGGTTGATGGTGCTCAAGTTTTTATCCTTTTTCCCTTCATGTCTTGTGATTTGGGTGGTTGAGGGAAAAGAAAGAAGCAAACTGAAAATAGAAGGAATCACTTAAAAAATTTGTCCTGTATGGATAGATATCATTTAGCGAAGACATCGTTGGTCTAAGTTGTTAGGCTTATGGTCTTTTCTGCACCCTTTAATTTGTATCTGGACTACATCTAAGATTACTCGGGATCGCTATGGTCAAATTCTAACCCTTCACATTGAGACTATCTTATTGTTCTGCAGTTCGCAATTATTAGTTCCCTAAGTGTAGCATTTTGGCCCACACTTCTTAAATACACATTTCTCTCACTGTTCAAGTACTGGCTGAAAGTGTCCTGTAGGCTGTAGCCAATACAATCAAGAAACAGAAATTGGTTTGTTGAGGTCAGTTTTTCGAGTTAGAAGACCACCGATGCATATCTGAACATAAAGGGGTTGAAGAACTTTCTTGAATGATGCTAAAATTGGAACGTTAGGCTGTAAATCCTGCAATTAATTTCTGATATGCTGGATGCAATCATTTCTATTTGGAAGCGGGACAATTACTTGTTCCATGCGTTTACCAATCTTCATCTAATGTCTAATGTAATGGACATATAATTTGTTCACTAAACTTAGAACTCATAATGTGTCGTTTTTGTCGTACTTTTTATTTACCACAGCTAGTAGATTTGGCGTAGGAAAGTTGATTTGCCATTGCCTGCCACTGACAATTGCTTTGTTGAAACTTATCACGTAGGTGACTGGAAATGATGGGGAGGTTGAAACAGCTCCAAAATCTTGGCTAACAATCATCATTCAGTTTATTGTTGCGATGCTCGTGTTGGACACTTGGCAATACTTCATTCACAGATACATGCATCAGAATAAGTTCCTATACAAGCACATCCATTCCCATCATCACCGGTTGGTTGTACCTTTTGCATTTGGAGCTTTATACAACCATCCATTGGAGGGTCTTCTCCTCGACACGATCGGTGGAGCTTTATCTTTCCTTGTCTCTGGGATGTCCTCTCGAGTCTCCATCTTTTTCTTTTCATTCGCCACCATCAAAACAGTAGATGACCATTGTGGGTTATGGATTCCCGGGAACCCTTTCCATGTGTTCTTCAGAAACAATTCTGCATATCATGATTTCCACCATCAGCTATATGGCAGTAAGTACAACTTCTCCCAGCCATTTTTTGTTATGTGGGATAGAATATTTGGGACCTATATGCCTTACTCAGAGAAGAGAGCCGGGGCTGGTTCGGAAGCGCGAGTTAAGGAAGATTAGGTGTTCCATAGCAAGCATATATTATTTTCCTCAGTGTTACTTGTTTTTTTTTTTTTTTTTTCCCTATCTCTCTCTCTCACTCTCACTGTTAGCCAGCCACCACACTGCTAAATTTGTATCATACGCCTACTCGTGTCTTACACTCTTGTTAGTATTATTTCCTCGTCGTTTCATAGCTGATTCGTGTTGTGATTTGAACCTTTTCTTTTTCTTGTACCTACTCCTTGTGAACAGAGCACCTCTGATTTTTCCTTCTTTGGATTTGGTTAAACGTTACTTATCCCTTTCCCAGCTGCATTCTGAACTATCTTTCTTCTACTGAAATAGATTGCTCTGATATGGTGAAGATGCAAATCTAGGCTAAACTTGGTTTCTAGGAAAATTTAGCACTTATTTGGTTCACATGGTATGATTAGTAGAACAAAGACACTGATATAATTTATGCATAAACATAGGAACTATATGTATTATTTAATGTCCAGTTGCTTCATTGAAACTATACATAACAGCAGGTAAAGTTTGGCAAACTGTCGGTCAAACATTGGATTAATAGCAGGAATCATATTTTGTTGTCTGTATCTGACCTGG

mRNA sequence

ATGGGTTTTGTATTCTCTGATCAAATGTTGGGTACTTTTGTGCCAATTGTGGTTTACTGGTTGTATTCTGGGATTTATATTCTACTGGGCTCATTTGAGAACTACCGGTTGCACTCCAAGGAAGATGAGCTAGAGAAGAACGTAGTGTCAAAGAGCACTGTGGTCAGAGGCGTTTTGCTTCAACAGACTATTCAGGCAGTCGTTGCGATCCTCTTGTTTAAGGTGACTGGAAATGATGGGGAGGTTGAAACAGCTCCAAAATCTTGGCTAACAATCATCATTCAGTTTATTGTTGCGATGCTCGTGTTGGACACTTGGCAATACTTCATTCACAGATACATGCATCAGAATAAGTTCCTATACAAGCACATCCATTCCCATCATCACCGGTTGGTTGTACCTTTTGCATTTGGAGCTTTATACAACCATCCATTGGAGGGTCTTCTCCTCGACACGATCGGTGGAGCTTTATCTTTCCTTGTCTCTGGGATGTCCTCTCGAGTCTCCATCTTTTTCTTTTCATTCGCCACCATCAAAACAGTAGATGACCATTGTGGGTTATGGATTCCCGGGAACCCTTTCCATGTGTTCTTCAGAAACAATTCTGCATATCATGATTTCCACCATCAGCTATATGGCAGTAAGTACAACTTCTCCCAGCCATTTTTTGTTATGTGGGATAGAATATTTGGGACCTATATGCCTTACTCAGAGAAGAGAGCCGGGGCTGGTTCGGAAGCGCGAGTTAAGGAAGATTAGGTGTTCCATAGCAAGCATATATTATTTTCCTCAGTGTTACTTGTTTTTTTTTTTTTTTTTTCCCTATCTCTCTCTCTCACTCTCACTGTTAGCCAGCCACCACACTGCTAAATTTGTATCATACGCCTACTCGTGTCTTACACTCTTGTTAGTATTATTTCCTCGTCGTTTCATAGCTGATTCGTGTTGTGATTTGAACCTTTTCTTTTTCTTGTACCTACTCCTTGTGAACAGAGCACCTCTGATTTTTCCTTCTTTGGATTTGGTTAAACGTTACTTATCCCTTTCCCAGCTGCATTCTGAACTATCTTTCTTCTACTGAAATAGATTGCTCTGATATGGTGAAGATGCAAATCTAGGCTAAACTTGGTTTCTAGGAAAATTTAGCACTTATTTGGTTCACATGGTATGATTAGTAGAACAAAGACACTGATATAATTTATGCATAAACATAGGAACTATATGTATTATTTAATGTCCAGTTGCTTCATTGAAACTATACATAACAGCAGGTAAAGTTTGGCAAACTGTCGGTCAAACATTGGATTAATAGCAGGAATCATATTTTGTTGTCTGTATCTGACCTGG

Coding sequence (CDS)

ATGGGTTTTGTATTCTCTGATCAAATGTTGGGTACTTTTGTGCCAATTGTGGTTTACTGGTTGTATTCTGGGATTTATATTCTACTGGGCTCATTTGAGAACTACCGGTTGCACTCCAAGGAAGATGAGCTAGAGAAGAACGTAGTGTCAAAGAGCACTGTGGTCAGAGGCGTTTTGCTTCAACAGACTATTCAGGCAGTCGTTGCGATCCTCTTGTTTAAGGTGACTGGAAATGATGGGGAGGTTGAAACAGCTCCAAAATCTTGGCTAACAATCATCATTCAGTTTATTGTTGCGATGCTCGTGTTGGACACTTGGCAATACTTCATTCACAGATACATGCATCAGAATAAGTTCCTATACAAGCACATCCATTCCCATCATCACCGGTTGGTTGTACCTTTTGCATTTGGAGCTTTATACAACCATCCATTGGAGGGTCTTCTCCTCGACACGATCGGTGGAGCTTTATCTTTCCTTGTCTCTGGGATGTCCTCTCGAGTCTCCATCTTTTTCTTTTCATTCGCCACCATCAAAACAGTAGATGACCATTGTGGGTTATGGATTCCCGGGAACCCTTTCCATGTGTTCTTCAGAAACAATTCTGCATATCATGATTTCCACCATCAGCTATATGGCAGTAAGTACAACTTCTCCCAGCCATTTTTTGTTATGTGGGATAGAATATTTGGGACCTATATGCCTTACTCAGAGAAGAGAGCCGGGGCTGGTTCGGAAGCGCGAGTTAAGGAAGATTAG

Protein sequence

MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLLQQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFLYKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKTVDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKRAGAGSEARVKED

Homology

[Close]

BLAST of CmaCh18G011580 vs. ExPASy Swiss-Prot
Match: Q9AST3 (Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 4.2e-109
Identity = 189/250 (75.60%), Postives = 218/250 (87.20%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           M FV SD+ LGTFVPI+VYW+YSG+YI LGS + YRLHSK DE EKN+VSKS VV+GVLL
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPK-SWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKF 120
           QQT+QA+++++LFK+TG+D +  T  + S L +  QFI+AMLV+DTWQYFIHRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIK 180
           LYKHIHS HHRL+VP+++GALYNHPLEGLLLDTIGGALSFL SGMS R +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYS-E 240
           TVDDHCGLW+PGNPFH+FF NNSAYHD HHQLYG+KYNFSQPFFVMWDRI GTY+PYS E
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRAGAGSEAR 249
           KRA  G E R
Sbjct: 242 KRANGGFETR 251

[Close]

BLAST of CmaCh18G011580 vs. ExPASy Swiss-Prot
Match: Q8VYI1 (Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 1.2e-108
Identity = 192/250 (76.80%), Postives = 218/250 (87.20%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MGF  SD++LGT  PIVVYWLYSGIY+ L S E+YRLHSK +E EKN+VSKS+VV+GVL+
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPK-SWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKF 120
           QQ +QAVVAILLF VTG+D E + A + S+L +  QF+ AM+VLDTWQYF+HRYMHQNKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIK 180
           LYKHIHS HHRL+VP+A+GALYNHP+EGLLLDTIGGALSFLVSGMS R SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYS-E 240
           TVDDHCGLW+PGN FH+ F+NNSAYHD HHQLYG+KYNFSQPFFVMWDRI GTYMPYS E
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGAGSEAR 249
           KR   G EAR
Sbjct: 243 KREDGGFEAR 252

[Close]

BLAST of CmaCh18G011580 vs. ExPASy Swiss-Prot
Match: B8B4W4 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39946 GN=GL1-9 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 7.8e-87
Identity = 159/255 (62.35%), Postives = 197/255 (77.25%), Query Frame = 0


Query: 2   GFVFSDQMLGTFVPIVVYWLYSGIYILL---GSFENYRLHSKEDELEKNVVSKSTVVRGV 61
           G+V SD+ +GTF PI +YW+Y+G Y L+      E YRLH++ +E EKN+V+   VVRGV
Sbjct: 6   GYV-SDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGV 65

Query: 62  LLQQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNK 121
           LLQQ +QA+VA++LF VT +   V   P   +    QF+VAMLV+D+WQYF+HRYMHQNK
Sbjct: 66  LLQQLVQAIVAMILFMVTSDSSAVVVQPPI-IIQAFQFLVAMLVMDSWQYFVHRYMHQNK 125

Query: 122 FLYKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATI 181
           FLY+HIHS HHRL+VP+A GALYNHPLEGLLLDT+GGA+SFLVSGM+ R S+FFF FA +
Sbjct: 126 FLYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVL 185

Query: 182 KTVDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYS- 241
           KTVDDHCGLW+P N F   F+NN+AYHD HHQL GSKYN+SQPFF +WDRI GT+MPY+ 
Sbjct: 186 KTVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNL 245

Query: 242 EKRAGAGSEARVKED 253
            +R   G EAR   D
Sbjct: 246 VRRKEGGFEARPLRD 258

[Close]

BLAST of CmaCh18G011580 vs. ExPASy Swiss-Prot
Match: Q0D4G3 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-9 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 7.8e-87
Identity = 159/255 (62.35%), Postives = 197/255 (77.25%), Query Frame = 0


Query: 2   GFVFSDQMLGTFVPIVVYWLYSGIYILL---GSFENYRLHSKEDELEKNVVSKSTVVRGV 61
           G+V SD+ +GTF PI +YW+Y+G Y L+      E YRLH++ +E EKN+V+   VVRGV
Sbjct: 6   GYV-SDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGV 65

Query: 62  LLQQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNK 121
           LLQQ +QA+VA++LF VT +   V   P   +    QF+VAMLV+D+WQYF+HRYMHQNK
Sbjct: 66  LLQQLVQAIVAMILFMVTSDSSAVVVQPPI-IIQAFQFLVAMLVMDSWQYFVHRYMHQNK 125

Query: 122 FLYKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATI 181
           FLY+HIHS HHRL+VP+A GALYNHPLEGLLLDT+GGA+SFLVSGM+ R S+FFF FA +
Sbjct: 126 FLYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVL 185

Query: 182 KTVDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYS- 241
           KTVDDHCGLW+P N F   F+NN+AYHD HHQL GSKYN+SQPFF +WDRI GT+MPY+ 
Sbjct: 186 KTVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNL 245

Query: 242 EKRAGAGSEARVKED 253
            +R   G EAR   D
Sbjct: 246 VRRKEGGFEARPLRD 258

[Close]

BLAST of CmaCh18G011580 vs. ExPASy Swiss-Prot
Match: O94298 (Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sur2 PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.2e-55
Identity = 111/253 (43.87%), Postives = 148/253 (58.50%), Query Frame = 0


Query: 15  PIVVYWLYSGI-----YILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLLQQTIQAVVA 74
           P+++YW+ S       YI L  FE YR+H  E+   +N V +  VV+ VL QQ  + VV 
Sbjct: 21  PVIIYWVASAFFGFLHYIELPVFEKYRIHPPEEIARRNRVPQMAVVKAVLFQQLCEVVVG 80

Query: 75  ILLFKVTGNDGEVET------------------------APKSWLTIII---QFIVAMLV 134
           I L    G    ++                         APK     I+   Q+  A  +
Sbjct: 81  IALAMFEGYPEPIDEAKQMLRYEAFFSKNLPALLQVAPFAPKLAYNFIVPAFQYFFAFFI 140

Query: 135 LDTWQYFIHRYMHQNKFLYKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVS 194
           +D+WQYF HRY+H NK LY  IH+HHHRL VP+A GALYNHP EGL+LDT G  +++L +
Sbjct: 141 IDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFEGLILDTFGAGVAYLAA 200

Query: 195 GMSSRVSIFFFSFATIKTVDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPF 236
           G+S + ++ FF+ +T+KTVDDHCG   P +P  +FF NN+ YHD HHQ YG + NFSQPF
Sbjct: 201 GLSPQQAVIFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDLHHQPYGFQKNFSQPF 260

[Close]

BLAST of CmaCh18G011580 vs. ExPASy TrEMBL
Match: A0A6J1HSN9 (sphinganine C4-monooxygenase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467426 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 4.4e-141
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL
Sbjct: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT
Sbjct: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240
           VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR
Sbjct: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240

Query: 241 AGAGSEARVKED 253
           AGAGSEARVKED
Sbjct: 241 AGAGSEARVKED 252

[Close]

BLAST of CmaCh18G011580 vs. ExPASy TrEMBL
Match: A0A6J1FZG3 (sphinganine C4-monooxygenase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449337 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 1.8e-139
Identity = 248/252 (98.41%), Postives = 250/252 (99.21%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVV KSTVVRGVLL
Sbjct: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVPKSTVVRGVLL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQTIQAVVAILLFKVTGNDGEV TAPKSWLTI++QFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAVVAILLFKVTGNDGEVATAPKSWLTIVLQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT
Sbjct: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240
           VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR
Sbjct: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240

Query: 241 AGAGSEARVKED 253
           AGAGSEARVKED
Sbjct: 241 AGAGSEARVKED 252

[Close]

BLAST of CmaCh18G011580 vs. ExPASy TrEMBL
Match: A0A0A0KQL6 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G605020 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.0e-126
Identity = 226/253 (89.33%), Postives = 237/253 (93.68%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MG V SDQ+LGTFVPIVVYWLYSG+YILLGSFENYRLHSK+DELEKN+VSKSTVVRGV L
Sbjct: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQ IQA+VAILLFKVT  DGEV T PKSWLTI++QFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKH+HS HHRLVVP+AFGALYNHPLEGLLLDTIGGALSFLVSGMS RV+IFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYS-EK 240
           VDDHCGLW+PGN FHVFFRNNSAYHD HHQLYGSKYNFSQPFFV WDRIFGTYMPYS EK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGAGSEARVKED 253
           RAG G EARVKED
Sbjct: 241 RAGGGFEARVKED 253

[Close]

BLAST of CmaCh18G011580 vs. ExPASy TrEMBL
Match: A0A5A7SZ77 (Sphinganine C4-monooxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00520 PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.2e-125
Identity = 226/253 (89.33%), Postives = 236/253 (93.28%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MG V SDQ+LGTFVPIVVYWLYSG+YILLGSFENYRLHSK+DELEKN+VSKSTVVRGV L
Sbjct: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQ IQA+VAILLFKVT  DGEV T PKSWLTI +QFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIAVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKH+HS HHRLVVP+AFGALYNHPLEGLLLDTIGGALSFLVSGMS RV+IFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYS-EK 240
           VDDHCGLW+PGN FHVFFRNNSAYHD HHQLYGSKYNFSQPFFV WDRIFGTYMPYS EK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGAGSEARVKED 253
           RAG G EARVKED
Sbjct: 241 RAGGGFEARVKED 253

[Close]

BLAST of CmaCh18G011580 vs. ExPASy TrEMBL
Match: A0A6J1DD01 (sphinganine C4-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111019225 PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.2e-125
Identity = 225/253 (88.93%), Postives = 236/253 (93.28%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MG V SDQ LGTFVPIVVYW+YSGIYILLGSFENYRLHSK+DELEKN+VSKSTVV GV L
Sbjct: 1   MGIVISDQFLGTFVPIVVYWVYSGIYILLGSFENYRLHSKKDELEKNLVSKSTVVGGVFL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQ IQA+VAILLFKVTGNDGEV + PK WLTII+QFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQIIQAIVAILLFKVTGNDGEVASGPKPWLTIIVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKH+HS HHRLVVP+AFGALYNHPLEGLLLDTIGGALSFL+SGMS RV+IFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLISGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYS-EK 240
           VDDHCGLW+PGN FHVFFRNNSAYHD HHQLYGSKYNFSQPFFV WDRIFGTYMPYS EK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RAGAGSEARVKED 253
           RAG G EARVKED
Sbjct: 241 RAGGGFEARVKED 253

[Close]

BLAST of CmaCh18G011580 vs. NCBI nr
Match: XP_022968087.1 (sphinganine C4-monooxygenase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 510.4 bits (1313), Expect = 9.0e-141
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL
Sbjct: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT
Sbjct: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240
           VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR
Sbjct: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240

Query: 241 AGAGSEARVKED 253
           AGAGSEARVKED
Sbjct: 241 AGAGSEARVKED 252

[Close]

BLAST of CmaCh18G011580 vs. NCBI nr
Match: XP_023542394.1 (sphinganine C4-monooxygenase 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 507.3 bits (1305), Expect = 7.6e-140
Identity = 249/252 (98.81%), Postives = 251/252 (99.60%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL
Sbjct: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQTIQA+VAILLFKVTGNDGEV TAPKSWLTII+QFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAIVAILLFKVTGNDGEVATAPKSWLTIIVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT
Sbjct: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240
           VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR
Sbjct: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240

Query: 241 AGAGSEARVKED 253
           AGAGSEARVKED
Sbjct: 241 AGAGSEARVKED 252

[Close]

BLAST of CmaCh18G011580 vs. NCBI nr
Match: KAG6574090.1 (Sphinganine C4-monooxygenase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 506.9 bits (1304), Expect = 1.0e-139
Identity = 249/252 (98.81%), Postives = 251/252 (99.60%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL
Sbjct: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQTIQAVVAILLFKVTGNDGEV TAPKSWLTI++QFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAVVAILLFKVTGNDGEVATAPKSWLTIVLQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT
Sbjct: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240
           VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR
Sbjct: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240

Query: 241 AGAGSEARVKED 253
           AGAGSEARVKED
Sbjct: 241 AGAGSEARVKED 252

[Close]

BLAST of CmaCh18G011580 vs. NCBI nr
Match: KAG7013149.1 (Sphinganine C4-monooxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 505.4 bits (1300), Expect = 2.9e-139
Identity = 248/252 (98.41%), Postives = 250/252 (99.21%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVV KSTVVRGVLL
Sbjct: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVPKSTVVRGVLL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQTIQAVVAILLFKVTGNDGEV TAPKSWLTI++QFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAVVAILLFKVTGNDGEVATAPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT
Sbjct: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240
           VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR
Sbjct: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240

Query: 241 AGAGSEARVKED 253
           AGAGSEARVKED
Sbjct: 241 AGAGSEARVKED 252

[Close]

BLAST of CmaCh18G011580 vs. NCBI nr
Match: XP_022944953.1 (sphinganine C4-monooxygenase 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 505.0 bits (1299), Expect = 3.8e-139
Identity = 248/252 (98.41%), Postives = 250/252 (99.21%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVV KSTVVRGVLL
Sbjct: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVPKSTVVRGVLL 60

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPKSWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKFL 120
           QQTIQAVVAILLFKVTGNDGEV TAPKSWLTI++QFIVAMLVLDTWQYFIHRYMHQNKFL
Sbjct: 61  QQTIQAVVAILLFKVTGNDGEVATAPKSWLTIVLQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180
           YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT
Sbjct: 121 YKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240
           VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR
Sbjct: 181 VDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYSEKR 240

Query: 241 AGAGSEARVKED 253
           AGAGSEARVKED
Sbjct: 241 AGAGSEARVKED 252

[Close]

BLAST of CmaCh18G011580 vs. TAIR 10
Match: AT1G14290.1 (sphingoid base hydroxylase 2 )

HSP 1 Score: 395.6 bits (1015), Expect = 3.0e-110
Identity = 189/250 (75.60%), Postives = 218/250 (87.20%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           M FV SD+ LGTFVPI+VYW+YSG+YI LGS + YRLHSK DE EKN+VSKS VV+GVLL
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPK-SWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKF 120
           QQT+QA+++++LFK+TG+D +  T  + S L +  QFI+AMLV+DTWQYFIHRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIK 180
           LYKHIHS HHRL+VP+++GALYNHPLEGLLLDTIGGALSFL SGMS R +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYS-E 240
           TVDDHCGLW+PGNPFH+FF NNSAYHD HHQLYG+KYNFSQPFFVMWDRI GTY+PYS E
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRAGAGSEAR 249
           KRA  G E R
Sbjct: 242 KRANGGFETR 251

[Close]

BLAST of CmaCh18G011580 vs. TAIR 10
Match: AT1G69640.1 (sphingoid base hydroxylase 1 )

HSP 1 Score: 394.0 bits (1011), Expect = 8.8e-110
Identity = 192/250 (76.80%), Postives = 218/250 (87.20%), Query Frame = 0


Query: 1   MGFVFSDQMLGTFVPIVVYWLYSGIYILLGSFENYRLHSKEDELEKNVVSKSTVVRGVLL 60
           MGF  SD++LGT  PIVVYWLYSGIY+ L S E+YRLHSK +E EKN+VSKS+VV+GVL+
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQTIQAVVAILLFKVTGNDGEVETAPK-SWLTIIIQFIVAMLVLDTWQYFIHRYMHQNKF 120
           QQ +QAVVAILLF VTG+D E + A + S+L +  QF+ AM+VLDTWQYF+HRYMHQNKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYKHIHSHHHRLVVPFAFGALYNHPLEGLLLDTIGGALSFLVSGMSSRVSIFFFSFATIK 180
           LYKHIHS HHRL+VP+A+GALYNHP+EGLLLDTIGGALSFLVSGMS R SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWIPGNPFHVFFRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGTYMPYS-E 240
           TVDDHCGLW+PGN FH+ F+NNSAYHD HHQLYG+KYNFSQPFFVMWDRI GTYMPYS E
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRAGAGSEAR 249
           KR   G EAR
Sbjct: 243 KREDGGFEAR 252

[Close]

BLAST of CmaCh18G011580 vs. TAIR 10
Match: AT2G29390.3 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 74.3 bits (181), Expect = 1.5e-13
Identity = 72/249 (28.92%), Postives = 111/249 (44.58%), Query Frame = 0


Query: 5   FSDQML---GTFVPIVVYWLYSGI-YILL---GSFENYRLHSKEDELEKNVVSKSTVVRG 64
           FSD  L   G+F+     +  SG+ YI L   G   NY++ +K +  E        + R 
Sbjct: 9   FSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARL 68

Query: 65  VLLQQTIQAVVAILLFKVTGNDGEVETAP-KSWLTIIIQFIVAMLVLDTWQYFIHRYMHQ 124
           +L    +   + +  + V    G   + P  SW  +  Q +   ++ D   Y+ HR +H 
Sbjct: 69  LLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILH- 128

Query: 125 NKFLYKHIHSHHHRLVVPFAFGALYNHPLEGLLL---DTIGGALSFLVSGMSSRVSIFFF 184
            K+LYK++HS HH    PF   + Y HP E L L     +G AL    +G        + 
Sbjct: 129 TKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWM 188

Query: 185 SFATIKTVDDHCGLWIPGNPFHVF-FRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGT 242
               I+TV+ HCG   P +P +       + +HD+HH+L  +K       FV  D IFGT
Sbjct: 189 MLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 248

[Close]

BLAST of CmaCh18G011580 vs. TAIR 10
Match: AT2G29390.2 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 74.3 bits (181), Expect = 1.5e-13
Identity = 72/249 (28.92%), Postives = 111/249 (44.58%), Query Frame = 0


Query: 5   FSDQML---GTFVPIVVYWLYSGI-YILL---GSFENYRLHSKEDELEKNVVSKSTVVRG 64
           FSD  L   G+F+     +  SG+ YI L   G   NY++ +K +  E        + R 
Sbjct: 16  FSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARL 75

Query: 65  VLLQQTIQAVVAILLFKVTGNDGEVETAP-KSWLTIIIQFIVAMLVLDTWQYFIHRYMHQ 124
           +L    +   + +  + V    G   + P  SW  +  Q +   ++ D   Y+ HR +H 
Sbjct: 76  LLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILH- 135

Query: 125 NKFLYKHIHSHHHRLVVPFAFGALYNHPLEGLLL---DTIGGALSFLVSGMSSRVSIFFF 184
            K+LYK++HS HH    PF   + Y HP E L L     +G AL    +G        + 
Sbjct: 136 TKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWM 195

Query: 185 SFATIKTVDDHCGLWIPGNPFHVF-FRNNSAYHDFHHQLYGSKYNFSQPFFVMWDRIFGT 242
               I+TV+ HCG   P +P +       + +HD+HH+L  +K       FV  D IFGT
Sbjct: 196 MLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 255

[Close]

BLAST of CmaCh18G011580 vs. TAIR 10
Match: AT2G29390.1 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 72.0 bits (175), Expect = 7.6e-13
Identity = 74/261 (28.35%), Postives = 114/261 (43.68%), Query Frame = 0


Query: 5   FSDQML---GTFVPIVVYWLYSGI-YILL---GSFENYRLHSKEDELEKNVVSKSTVVRG 64
           FSD  L   G+F+     +  SG+ YI L   G   NY++ +K +  E        + R 
Sbjct: 16  FSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARL 75

Query: 65  VLLQQTIQAVVAILLFKVTGNDGEVETAP-KSWLTIIIQFIVAMLVLDTWQYFIHRYMHQ 124
           +L    +   + +  + V    G   + P  SW  +  Q +   ++ D   Y+ HR +H 
Sbjct: 76  LLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRILH- 135

Query: 125 NKFLYKHIHSHHHRLVVPFAFGALYNHPLEGLLL---DTIGGALSFLVSGMSSRVSIFFF 184
            K+LYK++HS HH    PF   + Y HP E L L     +G AL    +G        + 
Sbjct: 136 TKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGPHLITLWLWM 195

Query: 185 SFATIKTVDDHCGL---WIPGNPFHVF----------FRNNSAYHDFHHQLYGSKYNFSQ 242
               I+TV+ HCG    W P N   ++          F  ++ +HD+HH+L  +K     
Sbjct: 196 MLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLYTKSGNYS 255

The following BLAST results are available for this feature:

BLAST of CmaCh18G011580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9AST3	4.2e-109	75.60	Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1	[more]
Q8VYI1	1.2e-108	76.80	Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1	[more]
B8B4W4	7.8e-87	62.35	Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39...	[more]
Q0D4G3	7.8e-87	62.35	Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=...	[more]
O94298	1.2e-55	43.87	Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]

BLAST of CmaCh18G011580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HSN9	4.4e-141	100.00	sphinganine C4-monooxygenase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1FZG3	1.8e-139	98.41	sphinganine C4-monooxygenase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A0A0KQL6	4.0e-126	89.33	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A5A7SZ77	1.2e-125	89.33	Sphinganine C4-monooxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1DD01	1.2e-125	88.93	sphinganine C4-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC1110192...	[more]

BLAST of CmaCh18G011580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022968087.1	9.0e-141	100.00	sphinganine C4-monooxygenase 2-like isoform X1 [Cucurbita maxima]	[more]
XP_023542394.1	7.6e-140	98.81	sphinganine C4-monooxygenase 2-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG6574090.1	1.0e-139	98.81	Sphinganine C4-monooxygenase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7013149.1	2.9e-139	98.41	Sphinganine C4-monooxygenase 2, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_022944953.1	3.8e-139	98.41	sphinganine C4-monooxygenase 2-like isoform X1 [Cucurbita moschata]	[more]

BLAST of CmaCh18G011580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14290.1	3.0e-110	75.60	sphingoid base hydroxylase 2	[more]
AT1G69640.1	8.8e-110	76.80	sphingoid base hydroxylase 1	[more]
AT2G29390.3	1.5e-13	28.92	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.2	1.5e-13	28.92	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.1	7.6e-13	28.35	sterol 4-alpha-methyl-oxidase 2-2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 96..232 e-value: 1.8E-25 score: 89.8
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 3..248
None	No IPR available	PANTHER	PTHR11863:SF122	SPHINGANINE C4-MONOOXYGENASE 1-LIKE	coord: 3..248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh18G011580.1	CmaCh18G011580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016491	oxidoreductase activity