CmaCh18G009700 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh18G009700 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 1.3e-193
Identity = 336/555 (60.54%), Postives = 416/555 (74.95%), Query Frame = 0

Query: 22  PPPPPPSAD-----LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSV 81
           PP  PPS        CKST YPKLCR+IL++++ SPSDPY YGKF++K CLKQA ++S V
Sbjct: 68  PPSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKV 127

Query: 82  IGDYLDR--GGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKGVDEELVERVQS 141
           I  Y  R           E GA+ DC +LS+ +V +L ++ + L+ A+ +   LVE V S
Sbjct: 128 ITSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNS 187

Query: 142 ILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKG 201
           +LS +VTN QTC+DGLVE++S    A+ +PM +  +LYS+SLGLV+++L+R    +R K 
Sbjct: 188 LLSGVVTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNR--NLKRFKA 247

Query: 202 DGGDSAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFINDTV 261
             G    G  +  REPL TLIKGL K  +C+    C    R L +LG T    I ++  V
Sbjct: 248 SKGKILGGGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAV 307

Query: 262 VVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIGDGI 321
           +V    +DNFT+I DAIA APNN+ P+DGYFVIY R G YEEY+VVP  K N+ML+GDGI
Sbjct: 308 IVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGI 367

Query: 322 NRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADLSTF 381
           N+T+ITGNH+VVDGWTTYN S+F V G+RF+A+DVTFRNTAGPEKHQAVALR++A+ S+F
Sbjct: 368 NKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSF 427

Query: 382 YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKIAIT 441
           YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAA+FQ CN+YARKP+  QK AIT
Sbjct: 428 YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAIT 487

Query: 442 AQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVYMQCYIGDLI 501
           A GR DPNQNTGISI +CTIKAAPD   +  ++ T  +LGRPWK YSRTV+MQ YI D++
Sbjct: 488 AHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMT--FLGRPWKPYSRTVFMQSYISDIV 547

Query: 502 SPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNFTVYNLTMGD 561
            PVGWLEWNGT GLDT+YYGE+ N+GPGANT+ RV W GY+L+N+ +AMNFTVYN TMGD
Sbjct: 548 QPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGD 607

Query: 562 TWLPYTDIPFSGGLV 565
           TWLP TDIPF GGL+
Sbjct: 608 TWLPQTDIPFYGGLL 616

BLAST of CmaCh18G009700 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 5.8e-191
Identity = 333/561 (59.36%), Postives = 410/561 (73.08%), Query Frame = 0

Query: 22  PPPPPPSAD-----------LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQA 81
           PP PPPS              CKST YPKLCR+ILS+++ SPSDPY YGKF++K CLKQA
Sbjct: 67  PPTPPPSQSPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQA 126

Query: 82  IKMSSVIGDYLDR--GGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKGVDEEL 141
            ++S VI  +  R           E  A+ DC +L++ +VE+L ++   L+AA+ +   L
Sbjct: 127 RRLSKVINRFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAAL 186

Query: 142 VERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWK 201
           V+RV S+L  +VTN QTC+DGLV+++S    A+  P+ +  +LYSVSLGLV+++L+R   
Sbjct: 187 VDRVTSLLGGVVTNQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNR--N 246

Query: 202 KRRMKGDGGDSAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GI 261
            +R KG  G    G     REPL TLIK L K  +C++   C    R L +LG T    I
Sbjct: 247 LKRYKGSKGKIFGGGNKPVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSI 306

Query: 262 FINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIM 321
            + + V V     DNF +I +A+A APN++ P+ GYFVIY R G YEEYVV+   K NIM
Sbjct: 307 LVREAVTVGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIM 366

Query: 322 LIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSS 381
           LIGDGIN+T+I+GNHS +DGWTTYNSSTF V GDRFVA+DVTFRNTAGPEKHQAVA+R++
Sbjct: 367 LIGDGINKTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNN 426

Query: 382 ADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPN 441
           AD STFYRCSFEGYQDTLYVHSLRQFYRECDIYGT+DFIFGNAAA+FQ CN+YARKP+ N
Sbjct: 427 ADGSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMAN 486

Query: 442 QKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVYMQC 501
           QK A+TA GRTDPNQ TGISI +CTI AAPD   D    +T  +LGRPWK YSRTVY+Q 
Sbjct: 487 QKNAVTAHGRTDPNQKTGISIINCTIGAAPDLAAD--PKSTMTFLGRPWKPYSRTVYIQS 546

Query: 502 YIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNFTVY 561
           YI D++ PVGWLEWNGT GLDT+ YGE++N+GPGA+TS RV W GYSL+N+ QAMNFTVY
Sbjct: 547 YISDVVQPVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVY 606

Query: 562 NLTMGDTWLPYTDIPFSGGLV 565
           N T+GDTWLP TDIPF GGL+
Sbjct: 607 NFTLGDTWLPQTDIPFYGGLL 621

BLAST of CmaCh18G009700 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 511.1 bits (1315), Expect = 1.6e-143
Identity = 285/581 (49.05%), Postives = 372/581 (64.03%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPP---PPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           M S+ +FL+ L L     F +     P   S+ +CK+T  PK C+S+    + S  D   
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVRE 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFL-RSIES 120
           YG+FS++  L Q+ K +  I  YL R  ++ L+Q   GAL+DCR L+    ++L  S E+
Sbjct: 61  YGRFSLRKSLTQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFET 120

Query: 121 V--LEAAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSS---LGNALTAPMSSAGQL 180
           V    ++K +     + +Q++LSA +TN QTC+DG+  + SS   + N +  P+ +  +L
Sbjct: 121 VNITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKL 180

Query: 181 YSVSLGLVTNSLSRLWKKRRMKGDGGDSAAGARN----REPLNTLIKGL--HKMGSCNQS 240
           +SVSL L T      W  ++ K     S A  +N     +P      G    KM    ++
Sbjct: 181 FSVSLALFTKG----WVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRA 240

Query: 241 TGCSGRKRLLA--DLGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIY 300
              S  +R LA  D     + ++D V V+  G  NFT+I +A+  APN +    GYFVIY
Sbjct: 241 VYESLSRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIY 300

Query: 301 VRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAID 360
           V  G YEE VV+ K K  +M+IGDGINRTV+TGN +VVDGWTT+NS+TF V    FVA++
Sbjct: 301 VTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVN 360

Query: 361 VTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIF 420
           +TFRNTAGPEKHQAVA+RSSADLS FY CSFE YQDTLY HSLRQFYRECDIYGTVDFIF
Sbjct: 361 MTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIF 420

Query: 421 GNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST 480
           GNAA VFQ CNLY R+P+ NQ  AITAQGRTDPNQNTGISIH+CTIK A D  +  SN T
Sbjct: 421 GNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVS--SNYT 480

Query: 481 TTNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMR 540
              YLGRPWK+YSRTV+MQ YI +++ PVGW EWNG   L TLYY E+ N G G++T+ R
Sbjct: 481 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 540

Query: 541 VDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFSGGLV 565
           V WPGY ++N T A NFTV N  +GD W+  + +P+  GL+
Sbjct: 541 VVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of CmaCh18G009700 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 9.7e-138
Identity = 275/585 (47.01%), Postives = 358/585 (61.20%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGK 60
           M S +  L+ LS        S      S+ +CK+T  PK C+S+      S  +   YG 
Sbjct: 1   MESPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGC 60

Query: 61  FSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREA-GALEDCRDLSDSNVEF-LRSIESVL 120
           FS++  L Q+ K    +  Y+ R  ++ L+Q     AL+DCR L+   +++ L S E+V 
Sbjct: 61  FSIRKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVN 120

Query: 121 EAA-----KGVDEELVERVQSILSAIVTNGQTCIDGLVESRS-----SLGNALTAPMSSA 180
           + +     K +     + +Q++LSA +TN QTC++GL  + S     ++   +  P+ + 
Sbjct: 121 DTSAKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVND 180

Query: 181 GQLYSVSLGLVTNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGL--HKMGSCNQST 240
            +L  VSL L T       KKR         +  + + +P      G    KM    ++ 
Sbjct: 181 TKLLGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAV 240

Query: 241 GCSGRKRLLADLGTTG-------IFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGY 300
             S  +R LAD  + G       + I+D V VS  G  NFT+I  A+A APNN+    G+
Sbjct: 241 YESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGF 300

Query: 301 FVIYVRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRF 360
           F+IYV  G YEEY+ + K K  +M+IGDGIN+TV+TGN SVVDGWTT+NS+TF V    F
Sbjct: 301 FLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNF 360

Query: 361 VAIDVTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTV 420
           VA+++TFRNTAGPEKHQAVALRS AD S FY CSFE YQDTLY HSLRQFYRECD+YGTV
Sbjct: 361 VAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTV 420

Query: 421 DFIFGNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDY 480
           DFIFGNAA VFQ CNLY RKP+PNQ  AITAQGR+DPNQNTG SI +CTIK A D  +  
Sbjct: 421 DFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVS-- 480

Query: 481 SNSTTTNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGAN 540
           SN T   YLGRPWK+YSRTVYMQ YI   + PVGW EWNG   L TLYY E+ N GPG+N
Sbjct: 481 SNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSN 540

Query: 541 TSMRVDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFSGGLV 565
           T+ RV WPGY ++N T A NFTV  L +   W+  T +P++ GL+
Sbjct: 541 TTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of CmaCh18G009700 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 483.8 bits (1244), Expect = 2.7e-135
Identity = 261/544 (47.98%), Postives = 334/544 (61.40%), Query Frame = 0

Query: 29  ADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGGDSR 88
           +++C+    P  CRS+L +    P D Y YG+ S++  L +A +  S+I   LDR G   
Sbjct: 36  SNICRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVA 95

Query: 89  LNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKGVDEELVERVQSILSAIVTNGQTCID 148
             +   GALEDC+ L+   +++L S     ++ K +     E V + LSA +TN QTC++
Sbjct: 96  A-KSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQTCLE 155

Query: 149 GLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKG-------DGGDSAA 208
           GL  + S   N L+  + +  +LY VSL L     S+ W  RR +               
Sbjct: 156 GLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQRSRPIWQPQARFKKFF 215

Query: 209 GARN-REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTTGIFINDTVVVSSTGADNFT 268
           G RN + PL        KM    ++   +  +R L       + ++D V V   G  NFT
Sbjct: 216 GFRNGKLPL--------KMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFT 275

Query: 269 SIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSV 328
           +I  AIA APN +   +GYF+IYV  G YEEYV VPK K  +M+IGDGIN+TVITGN SV
Sbjct: 276 TINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSV 335

Query: 329 VDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDT 388
           VDGWTT+NS+TF + G  F+ +++T RNTAGP K QAVALRS  DLS FY CSFE YQDT
Sbjct: 336 VDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDT 395

Query: 389 LYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNT 448
           LY HSLRQFYRECD+YGTVDFIFGNAA V Q CNLY R+P   Q   +TAQGRTDPNQNT
Sbjct: 396 LYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNT 455

Query: 449 GISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGT 508
           G +IH CTI+ A D  T  SN T   YLGRPWK+YSRTV MQ YI   + P GW  W+G 
Sbjct: 456 GTAIHGCTIRPADDLAT--SNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGD 515

Query: 509 AGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFS 565
             L TLYY E+ N GPG++T+ RV WPGY ++N T A NFTV N  +G+ W+  T +PF 
Sbjct: 516 FALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFV 559

BLAST of CmaCh18G009700 vs. ExPASy TrEMBL
Match: A0A6J1HU46 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467487 PE=3 SV=1)

HSP 1 Score: 1148.7 bits (2970), Expect = 0.0e+00
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGK 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGK
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGK 60

Query: 61  FSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEA 120
           FSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEA
Sbjct: 61  FSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEA 120

Query: 121 AKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVT 180
           AKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVT
Sbjct: 121 AKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVT 180

Query: 181 NSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGT 240
           NSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGT
Sbjct: 181 NSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGT 240

Query: 241 TGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKT 300
           TGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKT
Sbjct: 241 TGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVAL 360
           NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVAL
Sbjct: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVAL 360

Query: 361 RSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKP 420
           RSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKP
Sbjct: 361 RSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKP 420

Query: 421 LPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVY 480
           LPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVY
Sbjct: 421 LPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVY 480

Query: 481 MQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNF 540
           MQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNF
Sbjct: 481 MQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNF 540

Query: 541 TVYNLTMGDTWLPYTDIPFSGGLV 565
           TVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 TVYNLTMGDTWLPYTDIPFSGGLV 564

BLAST of CmaCh18G009700 vs. ExPASy TrEMBL
Match: A0A6J1G137 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449722 PE=3 SV=1)

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 559/565 (98.94%), Postives = 561/565 (99.29%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDS-PPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNSVLIFLLLLSLRRAGAFDS PPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVKNCLKQAIKMSSVIGDYLDRG DSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AAK VDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV
Sbjct: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240
           TNSLSRLWKKRRMKGDGGD AAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300
           TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYV+VPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVIVPKYK 300

Query: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360
           TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA
Sbjct: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420
           LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

Query: 421 PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV 480
           PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV
Sbjct: 421 PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV 480

Query: 481 YMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMN 540
           YMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTS+RVDWPGYSLMNITQAMN
Sbjct: 481 YMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMN 540

Query: 541 FTVYNLTMGDTWLPYTDIPFSGGLV 565
           FTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 FTVYNLTMGDTWLPYTDIPFSGGLV 565

BLAST of CmaCh18G009700 vs. ExPASy TrEMBL
Match: A0A6J1INW0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.4e-283
Identity = 488/567 (86.07%), Postives = 523/567 (92.24%), Query Frame = 0

Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNS   IFL LLSLR AGA DS   PPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDS---PPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSV+ CLKQAIKMSSVIG+YLDRGGDSRLN++E GALEDCR+LSDSNVEFL+SI+ VLE
Sbjct: 61  KFSVRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLE 120

Query: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AA+GVDEEL+ER++SILSAIVTNGQTCIDGLVESRSSLGNAL+AP+ SAG+LYSVSLGLV
Sbjct: 121 AAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGD--GGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLAD 240
            NSLSR WKKR  KGD  GG+  A A++REPLNTLIKGLH MGSCNQST CSGRKR+L D
Sbjct: 181 KNSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTD 240

Query: 241 LGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPK 300
           LGTTGI IN+TVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR GYY+EYVVVPK
Sbjct: 241 LGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPK 300

Query: 301 YKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQA 360
           YKTNIMLIGDGINRTVITGNH+VVDGWTTYNSSTFTV GD FVAID+TFRNTAG EKHQA
Sbjct: 301 YKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQA 360

Query: 361 VALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYA 420
           VA+R+SADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQ+CNLYA
Sbjct: 361 VAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYA 420

Query: 421 RKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSR 480
           RKPLPNQK A TAQGRTDPNQ+TGISIH+CTI AAP+W  D  N TTTNYLGRPWK +SR
Sbjct: 421 RKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDL-NETTTNYLGRPWKPFSR 480

Query: 481 TVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQA 540
           TVYMQ YIGDLISPVGWLEWNGT GLDTLYYGEFENYGPGANTS+RVDW G+SLMNIT+A
Sbjct: 481 TVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEA 540

Query: 541 MNFTVYNLTMGDTWLPYTDIPFSGGLV 565
           MNFTVYN TMGDTWLPYTDIPFSGGL+
Sbjct: 541 MNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of CmaCh18G009700 vs. ExPASy TrEMBL
Match: A0A6J1D8L4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1)

HSP 1 Score: 983.4 bits (2541), Expect = 3.9e-283
Identity = 491/565 (86.90%), Postives = 521/565 (92.21%), Query Frame = 0

Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNS+  IFLLLLSLR AGA DS   PPPSA LCK+TLYPKLC SILSSIRFSPSDPYGYG
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDS---PPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVK CLKQAIKMSSVIG YL RGG SRLNQREAGAL+DCR+L+D NVEFLRSIESVLE
Sbjct: 61  KFSVKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLE 120

Query: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AA+GVDE++VERV+SILSAIVTNGQTCIDGLVES+SSLG+AL+AP+S+AG+LYSVSLGLV
Sbjct: 121 AAEGVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240
            NSLSR WKKRR    GG       NREPL TLIKGL KMGSCNQST CSGR+RLL DLG
Sbjct: 181 KNSLSRRWKKRRQSFPGGGG-----NREPLITLIKGLRKMGSCNQSTICSGRERLLTDLG 240

Query: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300
           TTGIFIN+TVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYVR GYYEEYVVVPKYK
Sbjct: 241 TTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYK 300

Query: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360
           TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGD FVAIDVTFRNTAGPEKHQAVA
Sbjct: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420
           +R+SADLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQ+CNLYARK
Sbjct: 361 VRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV 480
           PLPNQK A TAQGRTDPNQ+TGISIH+CTI+AAPDW  D SN+T  NYLGRPWKQYSRTV
Sbjct: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMD-SNATIANYLGRPWKQYSRTV 480

Query: 481 YMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMN 540
           YMQ YIGDLISPVGWLEWNGTAGL+TLYYGEFEN+GPGANTSMRV+WPGYSLMN TQAMN
Sbjct: 481 YMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMN 540

Query: 541 FTVYNLTMGDTWLPYTDIPFSGGLV 565
           FTVYN TMGDTWLPYTDIPF+GGLV
Sbjct: 541 FTVYNFTMGDTWLPYTDIPFNGGLV 556

BLAST of CmaCh18G009700 vs. ExPASy TrEMBL
Match: A0A1S3BFR9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 5.7e-282
Identity = 489/567 (86.24%), Postives = 521/567 (91.89%), Query Frame = 0

Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNS+  IFLLLLSL  AGA  S   PP S  LCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVS---PPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVK C+KQAIKMS+VIGDYLDRG DSRLN+REAGAL DCR+LSD NVEFLRSIESVLE
Sbjct: 61  KFSVKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLE 120

Query: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AA+GVDEELVERV+S LSAIVTNGQTC+DGLVESRSSLG AL+AP+ SAG+LYSVSLGLV
Sbjct: 121 AAEGVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDG-GDSAAGARNREPLNTLIKGLHKMGSC-NQSTGCSGRKRLLAD 240
           +N++SR WKKRR KG G G    G R+RE LNTLIKGLHKM  C N ST CSGRKRLL D
Sbjct: 181 SNAMSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTD 240

Query: 241 LGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPK 300
           LG++GI IN+TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVR GYYEEYVVVPK
Sbjct: 241 LGSSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPK 300

Query: 301 YKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQA 360
           +KTNIMLIGDGINRT+ITGNH+VVDGWTTYNSSTFTVCGD FVAIDVTFRNTAGPEKHQA
Sbjct: 301 FKTNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQA 360

Query: 361 VALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYA 420
           VALR+SADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQ+CNLYA
Sbjct: 361 VALRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYA 420

Query: 421 RKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSR 480
           RKPLPNQK A TAQGRTDPNQNTGISIH+CTIKAAPDW  D SN+TTTNYLGRPWKQYSR
Sbjct: 421 RKPLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMD-SNTTTTNYLGRPWKQYSR 480

Query: 481 TVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQA 540
           TVYMQ YIGDLISPVGWLEWNGT GL+TLYYGE+ENYGPGANTS+RV+WPG+SLMN+TQA
Sbjct: 481 TVYMQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQA 540

Query: 541 MNFTVYNLTMGDTWLPYTDIPFSGGLV 565
           MNFTVYN TMGDTWLPYTDIPFSGGL+
Sbjct: 541 MNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of CmaCh18G009700 vs. NCBI nr
Match: XP_022968171.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima])

HSP 1 Score: 1148.7 bits (2970), Expect = 0.0e+00
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGK 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGK
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGK 60

Query: 61  FSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEA 120
           FSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEA
Sbjct: 61  FSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEA 120

Query: 121 AKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVT 180
           AKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVT
Sbjct: 121 AKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVT 180

Query: 181 NSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGT 240
           NSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGT
Sbjct: 181 NSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGT 240

Query: 241 TGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKT 300
           TGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKT
Sbjct: 241 TGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVAL 360
           NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVAL
Sbjct: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVAL 360

Query: 361 RSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKP 420
           RSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKP
Sbjct: 361 RSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKP 420

Query: 421 LPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVY 480
           LPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVY
Sbjct: 421 LPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVY 480

Query: 481 MQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNF 540
           MQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNF
Sbjct: 481 MQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNF 540

Query: 541 TVYNLTMGDTWLPYTDIPFSGGLV 565
           TVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 TVYNLTMGDTWLPYTDIPFSGGLV 564

BLAST of CmaCh18G009700 vs. NCBI nr
Match: XP_023541032.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 559/565 (98.94%), Postives = 562/565 (99.47%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDS-PPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNSVLIFLLLLSLRRAGAFDS PPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVKNCLKQAIKMSSVIGDYLDRG DSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AAK VDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV
Sbjct: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240
           TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300
           TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300

Query: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360
           TNIML+GDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA
Sbjct: 301 TNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420
           LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

Query: 421 PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV 480
           PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV
Sbjct: 421 PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV 480

Query: 481 YMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMN 540
           YMQCYIGDLI+PVGWLEWNGTAGLDTLYYGEFENYGPGANTS+RVDWPGYSLMNITQAMN
Sbjct: 481 YMQCYIGDLINPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMN 540

Query: 541 FTVYNLTMGDTWLPYTDIPFSGGLV 565
           FTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 FTVYNLTMGDTWLPYTDIPFSGGLV 565

BLAST of CmaCh18G009700 vs. NCBI nr
Match: XP_022945508.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita moschata] >XP_022945509.1 probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita moschata])

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 559/565 (98.94%), Postives = 561/565 (99.29%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDS-PPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNSVLIFLLLLSLRRAGAFDS PPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVKNCLKQAIKMSSVIGDYLDRG DSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AAK VDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV
Sbjct: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240
           TNSLSRLWKKRRMKGDGGD AAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300
           TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYV+VPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVIVPKYK 300

Query: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360
           TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA
Sbjct: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420
           LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

Query: 421 PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV 480
           PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV
Sbjct: 421 PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV 480

Query: 481 YMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMN 540
           YMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTS+RVDWPGYSLMNITQAMN
Sbjct: 481 YMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAMN 540

Query: 541 FTVYNLTMGDTWLPYTDIPFSGGLV 565
           FTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 FTVYNLTMGDTWLPYTDIPFSGGLV 565

BLAST of CmaCh18G009700 vs. NCBI nr
Match: KAG6573907.1 (putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 558/566 (98.59%), Postives = 560/566 (98.94%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDS--PPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSVLIFLLLLSLRRAGAFDS  PPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVKNCLKQAIKMSSVIGDYLDRG DSRLNQREAGALEDCRDLSDSNVEFLRSIESVL
Sbjct: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120

Query: 121 EAAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAAK VDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL
Sbjct: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240
           VTNSLSRLWKKRRMKGDGGD AAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL
Sbjct: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240

Query: 241 GTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300
           GTTGIFIN+TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY
Sbjct: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300

Query: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360
           KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV
Sbjct: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360

Query: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420
           ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR
Sbjct: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420

Query: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRT 480
           KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST TNYLGRPWKQYSRT
Sbjct: 421 KPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTATNYLGRPWKQYSRT 480

Query: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAM 540
           VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTS+RVDWPGYSLMNITQAM
Sbjct: 481 VYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSLRVDWPGYSLMNITQAM 540

Query: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 565
           NFTVYNLTMGDTWLPYTDIPFSGGLV
Sbjct: 541 NFTVYNLTMGDTWLPYTDIPFSGGLV 566

BLAST of CmaCh18G009700 vs. NCBI nr
Match: XP_022976564.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima])

HSP 1 Score: 984.9 bits (2545), Expect = 2.8e-283
Identity = 488/567 (86.07%), Postives = 523/567 (92.24%), Query Frame = 0

Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNS   IFL LLSLR AGA DS   PPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDS---PPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSV+ CLKQAIKMSSVIG+YLDRGGDSRLN++E GALEDCR+LSDSNVEFL+SI+ VLE
Sbjct: 61  KFSVRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLE 120

Query: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AA+GVDEEL+ER++SILSAIVTNGQTCIDGLVESRSSLGNAL+AP+ SAG+LYSVSLGLV
Sbjct: 121 AAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGD--GGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLAD 240
            NSLSR WKKR  KGD  GG+  A A++REPLNTLIKGLH MGSCNQST CSGRKR+L D
Sbjct: 181 KNSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTD 240

Query: 241 LGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPK 300
           LGTTGI IN+TVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR GYY+EYVVVPK
Sbjct: 241 LGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPK 300

Query: 301 YKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQA 360
           YKTNIMLIGDGINRTVITGNH+VVDGWTTYNSSTFTV GD FVAID+TFRNTAG EKHQA
Sbjct: 301 YKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQA 360

Query: 361 VALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYA 420
           VA+R+SADLSTFYRCSFEGYQDTLYVHSLRQFYRECD+YGTVDFIFGNAAAVFQ+CNLYA
Sbjct: 361 VAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYA 420

Query: 421 RKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSR 480
           RKPLPNQK A TAQGRTDPNQ+TGISIH+CTI AAP+W  D  N TTTNYLGRPWK +SR
Sbjct: 421 RKPLPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDL-NETTTNYLGRPWKPFSR 480

Query: 481 TVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQA 540
           TVYMQ YIGDLISPVGWLEWNGT GLDTLYYGEFENYGPGANTS+RVDW G+SLMNIT+A
Sbjct: 481 TVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEA 540

Query: 541 MNFTVYNLTMGDTWLPYTDIPFSGGLV 565
           MNFTVYN TMGDTWLPYTDIPFSGGL+
Sbjct: 541 MNFTVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of CmaCh18G009700 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 677.6 bits (1747), Expect = 8.9e-195
Identity = 336/555 (60.54%), Postives = 416/555 (74.95%), Query Frame = 0

Query: 22  PPPPPPSAD-----LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSV 81
           PP  PPS        CKST YPKLCR+IL++++ SPSDPY YGKF++K CLKQA ++S V
Sbjct: 68  PPSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKV 127

Query: 82  IGDYLDR--GGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKGVDEELVERVQS 141
           I  Y  R           E GA+ DC +LS+ +V +L ++ + L+ A+ +   LVE V S
Sbjct: 128 ITSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNS 187

Query: 142 ILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKG 201
           +LS +VTN QTC+DGLVE++S    A+ +PM +  +LYS+SLGLV+++L+R    +R K 
Sbjct: 188 LLSGVVTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNR--NLKRFKA 247

Query: 202 DGGDSAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFINDTV 261
             G    G  +  REPL TLIKGL K  +C+    C    R L +LG T    I ++  V
Sbjct: 248 SKGKILGGGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAV 307

Query: 262 VVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIGDGI 321
           +V    +DNFT+I DAIA APNN+ P+DGYFVIY R G YEEY+VVP  K N+ML+GDGI
Sbjct: 308 IVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGI 367

Query: 322 NRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADLSTF 381
           N+T+ITGNH+VVDGWTTYN S+F V G+RF+A+DVTFRNTAGPEKHQAVALR++A+ S+F
Sbjct: 368 NKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSF 427

Query: 382 YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKIAIT 441
           YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAA+FQ CN+YARKP+  QK AIT
Sbjct: 428 YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAIT 487

Query: 442 AQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVYMQCYIGDLI 501
           A GR DPNQNTGISI +CTIKAAPD   +  ++ T  +LGRPWK YSRTV+MQ YI D++
Sbjct: 488 AHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMT--FLGRPWKPYSRTVFMQSYISDIV 547

Query: 502 SPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNFTVYNLTMGD 561
            PVGWLEWNGT GLDT+YYGE+ N+GPGANT+ RV W GY+L+N+ +AMNFTVYN TMGD
Sbjct: 548 QPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGD 607

Query: 562 TWLPYTDIPFSGGLV 565
           TWLP TDIPF GGL+
Sbjct: 608 TWLPQTDIPFYGGLL 616

BLAST of CmaCh18G009700 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 668.7 bits (1724), Expect = 4.1e-192
Identity = 333/561 (59.36%), Postives = 410/561 (73.08%), Query Frame = 0

Query: 22  PPPPPPSAD-----------LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQA 81
           PP PPPS              CKST YPKLCR+ILS+++ SPSDPY YGKF++K CLKQA
Sbjct: 67  PPTPPPSQSPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQA 126

Query: 82  IKMSSVIGDYLDR--GGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKGVDEEL 141
            ++S VI  +  R           E  A+ DC +L++ +VE+L ++   L+AA+ +   L
Sbjct: 127 RRLSKVINRFAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAAL 186

Query: 142 VERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWK 201
           V+RV S+L  +VTN QTC+DGLV+++S    A+  P+ +  +LYSVSLGLV+++L+R   
Sbjct: 187 VDRVTSLLGGVVTNQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNR--N 246

Query: 202 KRRMKGDGGDSAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GI 261
            +R KG  G    G     REPL TLIK L K  +C++   C    R L +LG T    I
Sbjct: 247 LKRYKGSKGKIFGGGNKPVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSI 306

Query: 262 FINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIM 321
            + + V V     DNF +I +A+A APN++ P+ GYFVIY R G YEEYVV+   K NIM
Sbjct: 307 LVREAVTVGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIM 366

Query: 322 LIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSS 381
           LIGDGIN+T+I+GNHS +DGWTTYNSSTF V GDRFVA+DVTFRNTAGPEKHQAVA+R++
Sbjct: 367 LIGDGINKTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNN 426

Query: 382 ADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPN 441
           AD STFYRCSFEGYQDTLYVHSLRQFYRECDIYGT+DFIFGNAAA+FQ CN+YARKP+ N
Sbjct: 427 ADGSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMAN 486

Query: 442 QKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVYMQC 501
           QK A+TA GRTDPNQ TGISI +CTI AAPD   D    +T  +LGRPWK YSRTVY+Q 
Sbjct: 487 QKNAVTAHGRTDPNQKTGISIINCTIGAAPDLAAD--PKSTMTFLGRPWKPYSRTVYIQS 546

Query: 502 YIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNFTVY 561
           YI D++ PVGWLEWNGT GLDT+ YGE++N+GPGA+TS RV W GYSL+N+ QAMNFTVY
Sbjct: 547 YISDVVQPVGWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVY 606

Query: 562 NLTMGDTWLPYTDIPFSGGLV 565
           N T+GDTWLP TDIPF GGL+
Sbjct: 607 NFTLGDTWLPQTDIPFYGGLL 621

BLAST of CmaCh18G009700 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 511.1 bits (1315), Expect = 1.1e-144
Identity = 285/581 (49.05%), Postives = 372/581 (64.03%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPP---PPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           M S+ +FL+ L L     F +     P   S+ +CK+T  PK C+S+    + S  D   
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVRE 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFL-RSIES 120
           YG+FS++  L Q+ K +  I  YL R  ++ L+Q   GAL+DCR L+    ++L  S E+
Sbjct: 61  YGRFSLRKSLTQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFET 120

Query: 121 V--LEAAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSS---LGNALTAPMSSAGQL 180
           V    ++K +     + +Q++LSA +TN QTC+DG+  + SS   + N +  P+ +  +L
Sbjct: 121 VNITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKL 180

Query: 181 YSVSLGLVTNSLSRLWKKRRMKGDGGDSAAGARN----REPLNTLIKGL--HKMGSCNQS 240
           +SVSL L T      W  ++ K     S A  +N     +P      G    KM    ++
Sbjct: 181 FSVSLALFTKG----WVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRA 240

Query: 241 TGCSGRKRLLA--DLGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIY 300
              S  +R LA  D     + ++D V V+  G  NFT+I +A+  APN +    GYFVIY
Sbjct: 241 VYESLSRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIY 300

Query: 301 VRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAID 360
           V  G YEE VV+ K K  +M+IGDGINRTV+TGN +VVDGWTT+NS+TF V    FVA++
Sbjct: 301 VTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVN 360

Query: 361 VTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIF 420
           +TFRNTAGPEKHQAVA+RSSADLS FY CSFE YQDTLY HSLRQFYRECDIYGTVDFIF
Sbjct: 361 MTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIF 420

Query: 421 GNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNST 480
           GNAA VFQ CNLY R+P+ NQ  AITAQGRTDPNQNTGISIH+CTIK A D  +  SN T
Sbjct: 421 GNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVS--SNYT 480

Query: 481 TTNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMR 540
              YLGRPWK+YSRTV+MQ YI +++ PVGW EWNG   L TLYY E+ N G G++T+ R
Sbjct: 481 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 540

Query: 541 VDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFSGGLV 565
           V WPGY ++N T A NFTV N  +GD W+  + +P+  GL+
Sbjct: 541 VVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of CmaCh18G009700 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 491.9 bits (1265), Expect = 6.9e-139
Identity = 275/585 (47.01%), Postives = 358/585 (61.20%), Query Frame = 0

Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGK 60
           M S +  L+ LS        S      S+ +CK+T  PK C+S+      S  +   YG 
Sbjct: 1   MESPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGC 60

Query: 61  FSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREA-GALEDCRDLSDSNVEF-LRSIESVL 120
           FS++  L Q+ K    +  Y+ R  ++ L+Q     AL+DCR L+   +++ L S E+V 
Sbjct: 61  FSIRKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVN 120

Query: 121 EAA-----KGVDEELVERVQSILSAIVTNGQTCIDGLVESRS-----SLGNALTAPMSSA 180
           + +     K +     + +Q++LSA +TN QTC++GL  + S     ++   +  P+ + 
Sbjct: 121 DTSAKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVND 180

Query: 181 GQLYSVSLGLVTNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGL--HKMGSCNQST 240
            +L  VSL L T       KKR         +  + + +P      G    KM    ++ 
Sbjct: 181 TKLLGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAV 240

Query: 241 GCSGRKRLLADLGTTG-------IFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGY 300
             S  +R LAD  + G       + I+D V VS  G  NFT+I  A+A APNN+    G+
Sbjct: 241 YESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGF 300

Query: 301 FVIYVRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRF 360
           F+IYV  G YEEY+ + K K  +M+IGDGIN+TV+TGN SVVDGWTT+NS+TF V    F
Sbjct: 301 FLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNF 360

Query: 361 VAIDVTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTV 420
           VA+++TFRNTAGPEKHQAVALRS AD S FY CSFE YQDTLY HSLRQFYRECD+YGTV
Sbjct: 361 VAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTV 420

Query: 421 DFIFGNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDY 480
           DFIFGNAA VFQ CNLY RKP+PNQ  AITAQGR+DPNQNTG SI +CTIK A D  +  
Sbjct: 421 DFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVS-- 480

Query: 481 SNSTTTNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGAN 540
           SN T   YLGRPWK+YSRTVYMQ YI   + PVGW EWNG   L TLYY E+ N GPG+N
Sbjct: 481 SNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSN 540

Query: 541 TSMRVDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFSGGLV 565
           T+ RV WPGY ++N T A NFTV  L +   W+  T +P++ GL+
Sbjct: 541 TTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of CmaCh18G009700 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 483.8 bits (1244), Expect = 1.9e-136
Identity = 261/544 (47.98%), Postives = 334/544 (61.40%), Query Frame = 0

Query: 29  ADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGGDSR 88
           +++C+    P  CRS+L +    P D Y YG+ S++  L +A +  S+I   LDR G   
Sbjct: 36  SNICRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVA 95

Query: 89  LNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKGVDEELVERVQSILSAIVTNGQTCID 148
             +   GALEDC+ L+   +++L S     ++ K +     E V + LSA +TN QTC++
Sbjct: 96  A-KSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQTCLE 155

Query: 149 GLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKG-------DGGDSAA 208
           GL  + S   N L+  + +  +LY VSL L     S+ W  RR +               
Sbjct: 156 GLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQRSRPIWQPQARFKKFF 215

Query: 209 GARN-REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTTGIFINDTVVVSSTGADNFT 268
           G RN + PL        KM    ++   +  +R L       + ++D V V   G  NFT
Sbjct: 216 GFRNGKLPL--------KMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFT 275

Query: 269 SIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSV 328
           +I  AIA APN +   +GYF+IYV  G YEEYV VPK K  +M+IGDGIN+TVITGN SV
Sbjct: 276 TINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSV 335

Query: 329 VDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVALRSSADLSTFYRCSFEGYQDT 388
           VDGWTT+NS+TF + G  F+ +++T RNTAGP K QAVALRS  DLS FY CSFE YQDT
Sbjct: 336 VDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDT 395

Query: 389 LYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARKPLPNQKIAITAQGRTDPNQNT 448
           LY HSLRQFYRECD+YGTVDFIFGNAA V Q CNLY R+P   Q   +TAQGRTDPNQNT
Sbjct: 396 LYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNT 455

Query: 449 GISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTVYMQCYIGDLISPVGWLEWNGT 508
           G +IH CTI+ A D  T  SN T   YLGRPWK+YSRTV MQ YI   + P GW  W+G 
Sbjct: 456 GTAIHGCTIRPADDLAT--SNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGD 515

Query: 509 AGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFS 565
             L TLYY E+ N GPG++T+ RV WPGY ++N T A NFTV N  +G+ W+  T +PF 
Sbjct: 516 FALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFV 559