CmaCh18G003560 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G003560
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpolyphenol oxidase, chloroplastic-like
LocationCma_Chr18: 1928049 .. 1929770 (-)
RNA-Seq ExpressionCmaCh18G003560
SyntenyCmaCh18G003560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCACTCAAAACAATGGCTTCTATATCTCCTCTTCTATCCATTCTCCCATATTCCCAAATCAAAAACCAAACATCCAACCGTCAAAACAAAAGCCGTGGCATCAACCATGTTCCATGCAGAGCCTCCAATGGAGACTCCACAAGCAAGTTTGATAGAAGAGATGTGCTGTTTGGACTGGGCGGACTTTATGGTGCAGCTGCTCTAACCAACCAACAAAGCCCTGCGTCCGCAAAACCTTTAGCACCCGACGTCAACAACTGCGAGCCAGCCACAATAGTTGGCAACGAACACATCCCATGCTGCCCTCCCAAAATCCATGATATCATTGATTTCATCCATCCACCACACACAACAAAACTCCGCATTCGACAACCCGCCCATTCGCTTACTCCAAAGCAACAATTCAACTTCGAAAAAGCCATTCATATCATGAAACGCCTCTCCCCCAACGACCCTCGTAACTTCTACAGGCAAGCCGATGTTCATTGTGCCTATTGTGATTATGCCTACAAACAATTAGGCTTCCCAAACGCCGAAATGCAAGTCCATTGGAACGCTTTGTTCTTCCCATTTCACCGAGCTTACCTCTACTTCTTCGAGAGAATCTTGGGCCATTTAATCAACGAACCAGATTTCGCCATACCCTTTTGGAATTGGGATAATAAAAAGGGTATGCAAATGCCAGAAATCTACGACCACCCAAATTCGCCACTCTATGACAAGTTACGTAATCAAAGGCATAGGCCGCATAAATTGCTTAACCTTAACTACCATGGAGACGACAACCCTCCATACGACGTCGTGGATTGTAATCTCCGGTGGATGCATAAGCAAATGATAACGGACGCCGATACTGCTGAAGGGTTCCATGGAAAGCTACTCGTAGCATCTCAAGAAGCTAATGAAGAAACTGGCATGGGTACCATTGAGAGATCTCCTCATAATAATATTCACAATTGGGTTGGCGATCCAAACGCCAAGAACAATATGAACATGGGAGTGTTTTACTCGGCAGCTCGAGATCCGTTGTTTTATGGCCATCATGCGAATGTGGATCGCATGTGGAGCATTTGGCAAGGGTTAAAGAATGGGAAGCCAAGAGATTTTAATAACCCCGATTGGTTGAATTCCTCGTTTGTGTTCTATAATGAGCATAAACAAGCTGTACGCATCAAAGTTAGTGATTGTTTGGACACTAGAAGGCTTGGATATGTTTATCAATATGTAGATCTTCCATGGCTGCATACTAAGCAAACCCCACGTCTAAAAAAGCAGCTAAAAACAACGCAAGCTCCACAAGTTATTAAATTTCCTCAGGGTCTCAATTCAATGGTAACCACAACGGTGAAGAGACCGAAGAAATTAAGGGATGAAAAGGAAAAGAGTGAGAAAGATGAGGTATTGGTGATAGAAGGCATTGAATTGGATAAGAGATATTGGGTTCATTTTAATGTGCTTGTTAATGTTGTTGATGATGGCTCCGATGTGTGTCCGGGGAATACTGAGTTTGTAGGAACGTTTACGACCGTCCTGCATGGTCACAACCACCGTGGAAAGACGTCTTTACGAGTTGTGATAACGGAGGTGCTTAAAGAGTTGGGAATAGAGGACGATGAGAGTGTGATTGTGAGGCTTGACCCTAAGGTTGGTGGAGACAAGGTCACCATTGGAGGGATGAAGATCGAACTTCAGCCAAAAGGTCAGCCAAAAGGTCAGTAG

mRNA sequence

ATGCCACTCAAAACAATGGCTTCTATATCTCCTCTTCTATCCATTCTCCCATATTCCCAAATCAAAAACCAAACATCCAACCGTCAAAACAAAAGCCGTGGCATCAACCATGTTCCATGCAGAGCCTCCAATGGAGACTCCACAAGCAAGTTTGATAGAAGAGATGTGCTGTTTGGACTGGGCGGACTTTATGGTGCAGCTGCTCTAACCAACCAACAAAGCCCTGCGTCCGCAAAACCTTTAGCACCCGACGTCAACAACTGCGAGCCAGCCACAATAGTTGGCAACGAACACATCCCATGCTGCCCTCCCAAAATCCATGATATCATTGATTTCATCCATCCACCACACACAACAAAACTCCGCATTCGACAACCCGCCCATTCGCTTACTCCAAAGCAACAATTCAACTTCGAAAAAGCCATTCATATCATGAAACGCCTCTCCCCCAACGACCCTCGTAACTTCTACAGGCAAGCCGATGTTCATTGTGCCTATTGTGATTATGCCTACAAACAATTAGGCTTCCCAAACGCCGAAATGCAAGTCCATTGGAACGCTTTGTTCTTCCCATTTCACCGAGCTTACCTCTACTTCTTCGAGAGAATCTTGGGCCATTTAATCAACGAACCAGATTTCGCCATACCCTTTTGGAATTGGGATAATAAAAAGGGTATGCAAATGCCAGAAATCTACGACCACCCAAATTCGCCACTCTATGACAAGTTACGTAATCAAAGGCATAGGCCGCATAAATTGCTTAACCTTAACTACCATGGAGACGACAACCCTCCATACGACGTCGTGGATTGTAATCTCCGGTGGATGCATAAGCAAATGATAACGGACGCCGATACTGCTGAAGGGTTCCATGGAAAGCTACTCGTAGCATCTCAAGAAGCTAATGAAGAAACTGGCATGGGTACCATTGAGAGATCTCCTCATAATAATATTCACAATTGGGTTGGCGATCCAAACGCCAAGAACAATATGAACATGGGAGTGTTTTACTCGGCAGCTCGAGATCCGTTGTTTTATGGCCATCATGCGAATGTGGATCGCATGTGGAGCATTTGGCAAGGGTTAAAGAATGGGAAGCCAAGAGATTTTAATAACCCCGATTGGTTGAATTCCTCGTTTGTGTTCTATAATGAGCATAAACAAGCTGTACGCATCAAAGTTAGTGATTGTTTGGACACTAGAAGGCTTGGATATGTTTATCAATATGTAGATCTTCCATGGCTGCATACTAAGCAAACCCCACGTCTAAAAAAGCAGCTAAAAACAACGCAAGCTCCACAAGTTATTAAATTTCCTCAGGGTCTCAATTCAATGGTAACCACAACGGTGAAGAGACCGAAGAAATTAAGGGATGAAAAGGAAAAGAGTGAGAAAGATGAGGTATTGGTGATAGAAGGCATTGAATTGGATAAGAGATATTGGGTTCATTTTAATGTGCTTGTTAATGTTGTTGATGATGGCTCCGATGTGTGTCCGGGGAATACTGAGTTTGTAGGAACGTTTACGACCGTCCTGCATGGTCACAACCACCGTGGAAAGACGTCTTTACGAGTTGTGATAACGGAGGTGCTTAAAGAGTTGGGAATAGAGGACGATGAGAGTGTGATTGTGAGGCTTGACCCTAAGGTTGGTGGAGACAAGGTCACCATTGGAGGGATGAAGATCGAACTTCAGCCAAAAGGTCAGCCAAAAGGTCAGTAG

Coding sequence (CDS)

ATGCCACTCAAAACAATGGCTTCTATATCTCCTCTTCTATCCATTCTCCCATATTCCCAAATCAAAAACCAAACATCCAACCGTCAAAACAAAAGCCGTGGCATCAACCATGTTCCATGCAGAGCCTCCAATGGAGACTCCACAAGCAAGTTTGATAGAAGAGATGTGCTGTTTGGACTGGGCGGACTTTATGGTGCAGCTGCTCTAACCAACCAACAAAGCCCTGCGTCCGCAAAACCTTTAGCACCCGACGTCAACAACTGCGAGCCAGCCACAATAGTTGGCAACGAACACATCCCATGCTGCCCTCCCAAAATCCATGATATCATTGATTTCATCCATCCACCACACACAACAAAACTCCGCATTCGACAACCCGCCCATTCGCTTACTCCAAAGCAACAATTCAACTTCGAAAAAGCCATTCATATCATGAAACGCCTCTCCCCCAACGACCCTCGTAACTTCTACAGGCAAGCCGATGTTCATTGTGCCTATTGTGATTATGCCTACAAACAATTAGGCTTCCCAAACGCCGAAATGCAAGTCCATTGGAACGCTTTGTTCTTCCCATTTCACCGAGCTTACCTCTACTTCTTCGAGAGAATCTTGGGCCATTTAATCAACGAACCAGATTTCGCCATACCCTTTTGGAATTGGGATAATAAAAAGGGTATGCAAATGCCAGAAATCTACGACCACCCAAATTCGCCACTCTATGACAAGTTACGTAATCAAAGGCATAGGCCGCATAAATTGCTTAACCTTAACTACCATGGAGACGACAACCCTCCATACGACGTCGTGGATTGTAATCTCCGGTGGATGCATAAGCAAATGATAACGGACGCCGATACTGCTGAAGGGTTCCATGGAAAGCTACTCGTAGCATCTCAAGAAGCTAATGAAGAAACTGGCATGGGTACCATTGAGAGATCTCCTCATAATAATATTCACAATTGGGTTGGCGATCCAAACGCCAAGAACAATATGAACATGGGAGTGTTTTACTCGGCAGCTCGAGATCCGTTGTTTTATGGCCATCATGCGAATGTGGATCGCATGTGGAGCATTTGGCAAGGGTTAAAGAATGGGAAGCCAAGAGATTTTAATAACCCCGATTGGTTGAATTCCTCGTTTGTGTTCTATAATGAGCATAAACAAGCTGTACGCATCAAAGTTAGTGATTGTTTGGACACTAGAAGGCTTGGATATGTTTATCAATATGTAGATCTTCCATGGCTGCATACTAAGCAAACCCCACGTCTAAAAAAGCAGCTAAAAACAACGCAAGCTCCACAAGTTATTAAATTTCCTCAGGGTCTCAATTCAATGGTAACCACAACGGTGAAGAGACCGAAGAAATTAAGGGATGAAAAGGAAAAGAGTGAGAAAGATGAGGTATTGGTGATAGAAGGCATTGAATTGGATAAGAGATATTGGGTTCATTTTAATGTGCTTGTTAATGTTGTTGATGATGGCTCCGATGTGTGTCCGGGGAATACTGAGTTTGTAGGAACGTTTACGACCGTCCTGCATGGTCACAACCACCGTGGAAAGACGTCTTTACGAGTTGTGATAACGGAGGTGCTTAAAGAGTTGGGAATAGAGGACGATGAGAGTGTGATTGTGAGGCTTGACCCTAAGGTTGGTGGAGACAAGGTCACCATTGGAGGGATGAAGATCGAACTTCAGCCAAAAGGTCAGCCAAAAGGTCAGTAG

Protein sequence

MPLKTMASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYGAAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQPKGQPKGQ
Homology
BLAST of CmaCh18G003560 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.6e-138
Identity = 277/575 (48.17%), Postives = 367/575 (63.83%), Query Frame = 0

Query: 17  PYSQIKNQTSNRQNKSRGI-NHVPCRASNGD----STSKFDRRDVLFGLGGLYGAAALTN 76
           P+SQ  +Q S      R     V C+A+N D    + SK DRR+VL GLGGLYG A +  
Sbjct: 24  PFSQNNSQVSLLTKPKRSFARKVSCKATNNDQNDQAQSKLDRRNVLLGLGGLYGVAGMGT 83

Query: 77  QQSPASAKPLA-PDVNNCEPATI-VGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQPAHS 136
               A AKP+A PDV+ C PA +  G     CCPP    IIDF   P   KLRIR PAH+
Sbjct: 84  DPF-AFAKPIAPPDVSKCGPADLPQGAVPTNCCPPPSTKIIDF-KLPAPAKLRIRPPAHA 143

Query: 137 LTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALF 196
           +    +  + KA+ +MK L  +DPR+F +QA VHCAYCD AY Q+GFP  E+Q+H + LF
Sbjct: 144 VDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCAYCDGAYDQVGFPELELQIHNSWLF 203

Query: 197 FPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRNQRHR 256
           FPFHR YLYFFE+ILG LIN+P FA+PFWNWD+  GM +P IY  P SPLYDKLR+  H+
Sbjct: 204 FPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANHQ 263

Query: 257 PHKLLNLNYHG-DDNPPYD-VVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEETGM 316
           P  L++L+Y+G +DN   +  ++ NL+ M++QM++++  A+ F G    A  E   + G 
Sbjct: 264 PPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDE--PDPGG 323

Query: 317 GTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNGKP 376
           G+IE +PH  +H W GD    N  +MG FYSA RDP+F+ HH+NVDRMWSIW+ L  GK 
Sbjct: 324 GSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWSIWKTL-GGKR 383

Query: 377 RDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQL 436
            D  + DWL+S F+FYNE+ + VR+KV DCL+T+ LGYVYQ VD+PWL +K TPR  K  
Sbjct: 384 TDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVA 443

Query: 437 KTTQAPQV----------------IKFPQGLNSMVTTTVKRPK-KLRDEKEKSEKDEVLV 496
            +  A ++                 +FP  L S ++T VKRPK K R +K K +++E+LV
Sbjct: 444 LSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILV 503

Query: 497 IEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGK--TSLRVVIT 556
           IEGIE D+   V F+V VN VDD     P  TEF G+F +V H H H+ K  T LR+ +T
Sbjct: 504 IEGIEFDRDVAVKFDVYVNDVDD-LPSGPDKTEFAGSFVSVPHSHKHKKKMNTILRLGLT 563

Query: 557 EVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIE 564
           ++L+E+  EDD+SV+V L PK G   V IGG+KIE
Sbjct: 564 DLLEEIEAEDDDSVVVTLVPKFGA--VKIGGIKIE 590

BLAST of CmaCh18G003560 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 2.6e-130
Identity = 254/582 (43.64%), Postives = 357/582 (61.34%), Query Frame = 0

Query: 3   LKTMASISPLLSI---LPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFG 62
           + +++S S LL+     PY    ++ S R+   +  N    + ++ + T   DRR++L G
Sbjct: 1   MASLSSQSKLLATPYSFPYHTKPSRVSLRRVSCKASNDNKDKPNDQEKTFSIDRRNMLIG 60

Query: 63  LGGLYGAAALTNQQSPASAKPLAPDV--NNCE-PATIVGNEHIPCCPPKIHDIIDFIHPP 122
           LGGLYGA+ +        A P+ P V  ++C  P  +    ++ CCPP + D IDF  P 
Sbjct: 61  LGGLYGASNVFPSNQSTLAAPIQPPVLPDSCHPPEDLAEGVNVLCCPPDVKDPIDFQMPS 120

Query: 123 HTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGF 182
           + ++LRIR  AH   P     ++KA+  MK L  NDPR+FY+QA++HCAYC+ AY Q+GF
Sbjct: 121 NPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHCAYCNGAYDQVGF 180

Query: 183 PNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPN 242
           P+  +QVH + LF PFHR YLYF+ERILG LI++P FAIPFWNWD  KGM MP ++  PN
Sbjct: 181 PDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGMHMPHMFIDPN 240

Query: 243 SPLYDKLRNQRHRPHKLLNLNYHGDDNPPYD--VVDCNLRWMHKQMITDADTAEGFHGKL 302
           SPLYD  RN  H P  +++L++   + P ++   +  NL  M+KQ++  A  A  FHG+ 
Sbjct: 241 SPLYDAKRNPAHFPDTIVDLDFSSGEAPSHNPRQIGNNLSIMYKQVVR-AKKARLFHGRP 300

Query: 303 LVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDR 362
           L A     +E+G G++E +PH NIH W GDP   N  NMG FYSA RDPLFY HHANVDR
Sbjct: 301 LQAG-SFPDESGDGSLEGTPHGNIHLWSGDPRQSNFENMGNFYSAGRDPLFYAHHANVDR 360

Query: 363 MWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPW 422
           MW IW+     K  D+   DWLN+ F+ ++E+ Q VR+ V D LD R+LGY YQ VD+PW
Sbjct: 361 MWYIWKNSLGRK--DYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLGYAYQEVDIPW 420

Query: 423 LHTKQTPRLK-------KQLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDE 482
           +++K  PR         +   TT    + KFP  L+S V+  VKRPKK R + EK +++E
Sbjct: 421 INSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRPKKSRSKSEKEDEEE 480

Query: 483 VLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNH------RGKT 542
           VLVIE I+ + ++ + F+V +N  D+        TEF G+F  V H H H      R  T
Sbjct: 481 VLVIEKIKHEPQFPLKFDVYINDEDEDPSAA-DQTEFAGSFVNVPHFHRHGDKKDKRQTT 540

Query: 543 SLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIE 564
           +L + I+EVL EL ++ D+S++V L P+VG  ++TIGG KIE
Sbjct: 541 NLSIGISEVLDELDVDGDDSIVVTLVPRVGSGQITIGGAKIE 577

BLAST of CmaCh18G003560 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.3e-126
Identity = 256/588 (43.54%), Postives = 361/588 (61.39%), Query Frame = 0

Query: 13  LSILPYSQIKNQTSN---RQNKSRGI--NHVPCRASNGD------------STSKFDRRD 72
           +S  P S +  + S+    +N+SR    + V C ++NGD            S+ K DRR+
Sbjct: 20  ISAFPPSPLFQRASHVPVARNRSRRFAPSKVSCNSANGDPNSDSTSDVRETSSGKLDRRN 79

Query: 73  VLFGLGGLYGAAALTNQQSP-ASAKPL-APDVNNCEPATIV-GNEHIPCCPPKIHDIIDF 132
           VL G+GGLYGAA       P A   P+ APD++ C  AT+  G     CCPP    IIDF
Sbjct: 80  VLLGIGGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDF 139

Query: 133 IHPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYK 192
             P   + +R R  AH ++ +    ++KAI + K L  +DPR+F +QA+VHC YC  AY 
Sbjct: 140 QLPSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYD 199

Query: 193 QLGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIY 252
           Q+G+ + E+QVH + LF PFHR YLYF ERIL  LI++P FA+P+W WDN  GM MP IY
Sbjct: 200 QVGYTDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIY 259

Query: 253 DHPNSPLYDKLRNQRHRPHKLLNLNYHGDDN--PPYDVVDCNLRWMHKQMITDADTAEGF 312
               S LYD+ RN +H P  +++L+Y G +   P  ++   NL  M+KQ+++ A T + F
Sbjct: 260 ASSPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLF 319

Query: 313 HGKLLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHA 372
            G    A      + G GT+E +PHN +H W G  + K + +MG FY+A RDP+F+GHHA
Sbjct: 320 LGYPYRAGDAI--DPGAGTLEHAPHNIVHKWTGLAD-KPSEDMGNFYTAGRDPIFFGHHA 379

Query: 373 NVDRMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYV 432
           NVDRMW+IW+ +     +DF + DWL+++FVFY+E+KQ V++KVSDC+DT +L Y YQ +
Sbjct: 380 NVDRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDI 439

Query: 433 DLPWL------HTKQTPRLKK-------QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLR 492
            +PWL        K T +  K       +L  T    +  FP+ LNS++   V RPKK R
Sbjct: 440 PIPWLPKNTKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSR 499

Query: 493 DEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGH-- 552
            +KEK +++EVL+I+GIELD+  +V F+V +N  +D S   P N+EF G+F  V H H  
Sbjct: 500 SKKEKEDEEEVLLIKGIELDRENFVKFDVYIN-DEDYSVSRPKNSEFAGSFVNVPHKHMK 559

Query: 553 NHRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIE 564
             + KT+LR  I E+L++LG EDDESVIV + P+ GGD VTIGG++IE
Sbjct: 560 EMKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIE 603

BLAST of CmaCh18G003560 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 452.6 bits (1163), Expect = 6.6e-126
Identity = 261/579 (45.08%), Postives = 363/579 (62.69%), Query Frame = 0

Query: 4   KTMASISPLLSILPY-SQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGG 63
           K+++S + + S LP  SQI   T+ R +  +    V C A NGDS  K DRRDVL GLGG
Sbjct: 18  KSLSSSATISSPLPKPSQIHIATAKRTHHFK----VSCNAPNGDSQPKLDRRDVLLGLGG 77

Query: 64  LYGAAALTNQQSPASAKPL-APDVNNC-------EPATIVGNEHIPCCPPKIHDIIDFIH 123
           L GAA+L N    A A+P+ AP+++ C        P  IV N    CCPP   ++I +  
Sbjct: 78  LAGAASLINNPL-AFAEPIHAPEISKCVVPPKDLPPDAIVDN----CCPPLATNVIPYKV 137

Query: 124 PPHT-TKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQ 183
           P  + + ++IR   H +  +    FEKAI +MK L  +DPRNFY+QA VHCAYC+  Y Q
Sbjct: 138 PKTSPSAMKIRPAIHRMDKEYIAKFEKAIRLMKELPADDPRNFYQQALVHCAYCNGGYVQ 197

Query: 184 LGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYD 243
             +P+ E+QVH + LFFPFHR YLYF+ERILG LI +P F +PFWNWD   GM +P+ + 
Sbjct: 198 TDYPDKEIQVHNSWLFFPFHRWYLYFYERILGKLIGDPTFGLPFWNWDTPAGMLIPQYFR 257

Query: 244 HPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDV--VDCNLRWMHKQMITDADTAEGFH 303
           + NSPLYD+ RNQ H P  +++L Y G D    D   +  NL  M+K M+T+A TAE F 
Sbjct: 258 NQNSPLYDENRNQSHLP-LVMDLGYAGTDTDVTDQERISNNLALMYKSMVTNAGTAELFL 317

Query: 304 GKLLVASQEANEETGMGTIERSPHNNIHNWVGD--PNAKNNMNMGVFYSAARDPLFYGHH 363
           GK   A  +   + G G+IE  PH  +H WVGD  P  +N  +MG FYSA RD LFY HH
Sbjct: 318 GKPYKAGDDPVNKGG-GSIENIPHTPVHRWVGDVKPRTQNGEDMGNFYSAGRDILFYCHH 377

Query: 364 ANVDRMWSIWQGL-KNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQ 423
           +NVDRMW+IWQ L   G+ RDF + DWL+++F+FY+E+KQAVR++V D LD ++LGY Y+
Sbjct: 378 SNVDRMWTIWQQLGGKGRRRDFTDSDWLDATFIFYDENKQAVRVRVGDALDNQKLGYKYE 437

Query: 424 YVDLPWLHTKQTPRLKKQ--LKTTQAPQVIK-FPQGLNSMVTTTVKRPKKLRDEKEKSEK 483
           + +LPWL++K  P  KK      ++AP V   FP  L+ +V   V RPKK R ++EK  +
Sbjct: 438 FTNLPWLNSKPLPTKKKTGLAARSKAPFVTDVFPLTLDKVVQVKVPRPKKSRSKEEKEAE 497

Query: 484 DEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGH--NHRGKTSL 543
           +E+L I+GIE+    +  F+V +N  +D  +      E+ G+F  + H H  + + +TSL
Sbjct: 498 EEILEIQGIEVAIDQYAKFDVYLN-DEDEPEAGKEKAEYAGSFAHLPHKHTGSKKIRTSL 557

Query: 544 RVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKI 563
            + + E L++LG EDD++V+V L PKVGG  VT+  +KI
Sbjct: 558 SLGLNEPLEDLGAEDDDAVLVTLAPKVGGGVVTVENIKI 584

BLAST of CmaCh18G003560 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.5e-125
Identity = 257/590 (43.56%), Postives = 354/590 (60.00%), Query Frame = 0

Query: 14  SILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFD---------RRDVLFGLGGLY 73
           S+ P+ Q ++Q+S  +   R    V C ++N  +  K +         RR+VL GLGG+Y
Sbjct: 25  SVYPFLQKQHQSSKLRKPKR---QVTCSSNNNQNNPKEEQELSNIVGHRRNVLIGLGGIY 84

Query: 74  GAAALTNQQSPASAKPLA-PDVNNCEPATIVGN----EHIPCCPPKIHDIIDFIHPPHTT 133
           G  A TN    A A P++ PD++ C P + + +     +I CCPP    I DF  P +  
Sbjct: 85  GTLA-TNPS--ALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSN-Q 144

Query: 134 KLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNA 193
            LR+RQ AH +  +    ++KA  +MK L  NDPRNF +QA++HCAYCD AY Q+GFP+ 
Sbjct: 145 PLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGFPDL 204

Query: 194 EMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPL 253
           ++QVH + LFFPFHR YLYF+ERILG LIN+P FA+PFWN+D   GMQ+P IY    SPL
Sbjct: 205 KLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPL 264

Query: 254 YDKLRNQRHRPHKLLNLNY--HGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVA 313
           YD+LRN  H+P  L++LN+   G D    +++  NL  M++Q+ ++  T+  F G    A
Sbjct: 265 YDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRA 324

Query: 314 SQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWS 373
                E  G G+IE  PH  +H W GD    N  +MG+FYSAARDP+FY HH+NVDR+W 
Sbjct: 325 GDA--EPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWY 384

Query: 374 IWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHT 433
           IW+ L  GK  DF + DWL S F+FY+E+K  VR+ V D LD  +LGY YQ V +PW   
Sbjct: 385 IWKTL-GGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKA 444

Query: 434 KQTPRLKKQLKTT--------------------QAP-QVIKFPQGLNSMVTTTVKRPKKL 493
           K  PR  K  K                      Q+P + + FP  LN+ V+  VKRPKKL
Sbjct: 445 KPVPRRTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKKL 504

Query: 494 RDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLH-GH 553
           R +KEK E++EVLVIEGIE      + F+V +N  D+   V  GNTEF G+F  + H  H
Sbjct: 505 RSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYIN--DEDDKVGAGNTEFAGSFVNIPHSAH 564

Query: 554 NHRGK---TSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKI 563
            H+ K   TSLR+ IT++L++L +E D++++V L PK G  +V I  ++I
Sbjct: 565 GHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEI 602

BLAST of CmaCh18G003560 vs. ExPASy TrEMBL
Match: A0A6J1K2N9 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111490108 PE=3 SV=1)

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 125
           AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ
Sbjct: 61  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 305
           QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIELQPKGQPKGQ 574
           LDPKVGGDKVTIGGMKIELQPKGQPKGQ
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGQPKGQ 568

BLAST of CmaCh18G003560 vs. ExPASy TrEMBL
Match: A0A6J1GRK5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456826 PE=3 SV=1)

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 537/564 (95.21%), Postives = 546/564 (96.81%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSN QNKSRGIN VPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINQVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 125
           AAALTNQQSPASA PLAPDVNNCE ATI GNEHIPCCPPKIHDIIDFIHPPHTTKLRIR 
Sbjct: 61  AAALTNQQSPASAAPLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRP 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRL PNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEP+F IPFWNWDN +GMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 305
           QRHRPHKLLNLNYHGDDNP YDVVD NLRWMHKQMITDA+TAEGFHGKLL+AS EANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIE+SPHNNIHNWVGDPNAKN+MNMG FYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIEKSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           K RDF+NPDWLNSSFVFYNEHKQAVRIKV DCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRP KL +EKEKSEKDEVLVIEGIELDKR+WVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTV HGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIELQPKGQ 570
           LDPKVGGDKVTIGGMKIELQPKGQ
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGQ 564

BLAST of CmaCh18G003560 vs. ExPASy TrEMBL
Match: A0A172DC01 (Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 3.5e-194
Identity = 348/577 (60.31%), Postives = 429/577 (74.35%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASN------------GDSTSKFDR 65
           MAS+S LLS+ P  + KNQ+S+R N+  G++++PCRASN            GDS SKFDR
Sbjct: 1   MASLSSLLSLFPCIRSKNQSSSRGNRRLGLSYIPCRASNGVQTPMNSNPQEGDSMSKFDR 60

Query: 66  RDVLFGLGGLYGAAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFI 125
           RDVLFGLGGLY AAAL   +S A A P++PDV +CEP T+  +E + CCPP I DI DF+
Sbjct: 61  RDVLFGLGGLYSAAALGRDRS-ALADPISPDVIHCEPITLEADEKVDCCPPSILDITDFV 120

Query: 126 HPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQ 185
            PP    LR+R  AH +T +Q    E+A+  MK L P+DPRNFY+QA VHCAYC+ AYKQ
Sbjct: 121 -PPAVENLRVRPAAHLITQEQLAKLEEAVTKMKALDPDDPRNFYQQARVHCAYCNDAYKQ 180

Query: 186 LGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIY- 245
            GFP  +  +HWN LFFPFHR YLYF ERILG LIN+PDFAIPFWNWD   GMQMP IY 
Sbjct: 181 DGFPELDFDIHWNWLFFPFHRCYLYFHERILGSLINDPDFAIPFWNWDAIPGMQMPAIYI 240

Query: 246 -DHPNSPLYDKLRNQRHRPHKLLNLNYHG-DDNPPYDVVDCNLRWMHKQMITDADTAEGF 305
            D  NSPLYD+LR+  H+P KLL+LNY G  ++P YDV+ CN R M++QM+ ++ TAEGF
Sbjct: 241 NDDKNSPLYDELRDPNHKPPKLLDLNYDGATEDPSYDVLFCNPRLMYRQMVINSKTAEGF 300

Query: 306 HGKLLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHA 365
           HGK L A QEA   TGMG+IE S HNN+H W GDP  K   +MG FY+AARDPLFY HHA
Sbjct: 301 HGKPLRAGQEA-YRTGMGSIELSAHNNVHTWSGDPQQKYREDMGHFYAAARDPLFYAHHA 360

Query: 366 NVDRMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYV 425
           NVDRMW+IWQGL +GK RDF +PDWLN+SFVFY+E+K A+RIKV DCLD  +LGY YQ V
Sbjct: 361 NVDRMWTIWQGLIDGKSRDFTDPDWLNASFVFYDENKNAIRIKVQDCLDNEKLGYKYQAV 420

Query: 426 DLPWLHTKQTPRLK-KQLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVL 485
           ++PWLHTK  PR K  QLK+++A +     + LNS +T  VKRP++ R + +KS +DEVL
Sbjct: 421 EIPWLHTKPIPRQKTSQLKSSKAQETPPI-EVLNSKMTKEVKRPQRSRSKTQKSVEDEVL 480

Query: 486 VIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITE 545
           VIE +ELD    VHFNVLVNV+DDG+DV PGNTEF G+F ++  GH HR  TSL +VITE
Sbjct: 481 VIE-MELDTTSRVHFNVLVNVLDDGTDVSPGNTEFAGSFKSIPLGHKHRANTSLSLVITE 540

Query: 546 VLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 567
           +++ELG +DDE+V V +DPK+GGD VTIGG+KIELQP
Sbjct: 541 LIEELGYDDDETVFVTIDPKLGGDLVTIGGIKIELQP 572

BLAST of CmaCh18G003560 vs. ExPASy TrEMBL
Match: A0A5A7UTU0 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00530 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 3.4e-157
Identity = 309/569 (54.31%), Postives = 394/569 (69.24%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTS-NRQNKSRGI-NHVPCRASNG--DSTSKFDRRDVLFGLG 65
           MAS+S LLS+ PYS  K Q S    NK RG+ NHV C+ASN   DS +KFDRRD+LFGLG
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCKASNNGEDSVNKFDRRDMLFGLG 60

Query: 66  GLYGAAALTNQQ-SPASAKPLAPDVNNCEPA--TIVGNEHIPCCPPKIHDIIDFIHPPHT 125
           GLYG A L+ +  + A    L+PDV+NC+P   + VG + + CCPPKI +II+F  PP  
Sbjct: 61  GLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKK-MDCCPPKIKEIIEFT-PPVV 120

Query: 126 TKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPN 185
              R+R   H +TP+Q  + EK I +MK L  +DPR+F  QA VHCAYCD AYKQ+    
Sbjct: 121 PTPRVRPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMD-ST 180

Query: 186 AEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSP 245
            + QVH NALFFPFHRAYLYFFERIL HL+ +PDFAIPFWNWD   GM MP+IY  P+S 
Sbjct: 181 MDFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSSS 240

Query: 246 LYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVAS 305
           LYD+ R++ H+P KLLNLNY G +    ++VD NLRWM+ QM    +TA+ F GK  V  
Sbjct: 241 LYDENRDKCHQPPKLLNLNYGGKEVKDPEIVDFNLRWMNDQM--SVETAKEFLGK--VIK 300

Query: 306 QEANEETGMGTIERSPHNNIHNWVGDPNAK-NNMNMGVFYSAARDPLFYGHHANVDRMWS 365
           +    E G G+IE SPHNNIH WVGDP A  +  +MG FYSA RDPLFY HHANVDRMW+
Sbjct: 301 RGNGPENGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWN 360

Query: 366 IWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHT 425
           IWQ +K+ +PRD  + DWLNSSFV Y+E+++AVRIKVS CLD++ LGYVYQ V +PW  T
Sbjct: 361 IWQKMKD-RPRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDT 420

Query: 426 KQTPRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELD 485
               R K++      P  I FPQ L+S +TT V RP ++   +E+  K+EVLV+E +E D
Sbjct: 421 PPIRRPKQERSLGNLPPTIDFPQILSSDITTMVDRP-EVASPEERINKNEVLVLEDVEYD 480

Query: 486 KRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIE 545
               +HF+VL+NV D+G DV  G+ EFVGTFT V HG     KTSL  VIT+ +++LG+E
Sbjct: 481 PTLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLE 540

Query: 546 DDESVIVRLDPKV-GGDKVTIGGMKIELQ 566
            D+ V V++ P V GGD++ IGG+KI+L+
Sbjct: 541 GDKKVAVKIVPNVNGGDRIKIGGIKIQLE 560

BLAST of CmaCh18G003560 vs. ExPASy TrEMBL
Match: A0A0A0LUI1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 5.8e-157
Identity = 301/569 (52.90%), Postives = 384/569 (67.49%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNRQNKSR-GINHVPCRA--SNG-DSTSKFDRRDVLFGLG 65
           MAS+S LL +  YSQ K ++S   NK    +N+V C+A  +NG DS SKFDRR+VLFGLG
Sbjct: 1   MASLSSLLLLSSYSQTKTKSSISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGLG 60

Query: 66  GLYGAAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKL 125
           GLYG    T   S   A PL PD+ NC  A   G     CCPP+I  II F+ PP   + 
Sbjct: 61  GLYGVVTAT---SSVLAAPLTPDIFNCHMAKTPGGIK-DCCPPQIGKIIHFV-PPKIQQP 120

Query: 126 RIRQP-AHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLG-FPNA 185
            +R+P AH +   Q    E  I  +K+L   DP +F  QA +HCAYCDY Y QLG     
Sbjct: 121 YVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITET 180

Query: 186 EMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPL 245
              VH NALFFPFHRAYLYFFERIL H I  P+FAIPFWNWD+  GM MPE Y+ P+S L
Sbjct: 181 RFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSL 240

Query: 246 YDKLRNQRHRPHKLLNLNYHG-DDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVAS 305
           YD LR+ RH PHKL+ LNY+G  ++  +DVVDCNLRWM+ +M     +   F G++L A 
Sbjct: 241 YDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRM--QVQSPRSFLGRVLKAG 300

Query: 306 QEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSI 365
           +  N  T MGTIE+SPHNNIH+W+G     +  +MG FYSAA DPLFY HHAN+DR+W+I
Sbjct: 301 ETPN-PTDMGTIEQSPHNNIHDWLG-TKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNI 360

Query: 366 WQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTK 425
           W+ L +G P+D  +PDWLNSSFVFY+E+K AV IKVSDCLDT +LGYVYQ V LPWLH  
Sbjct: 361 WKTL-DGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMN 420

Query: 426 QTPRLKKQLKTTQA-PQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELD 485
           + P     ++T ++ P ++ FPQ LNS +TT V RP+   +++   E DEVLV+E +E D
Sbjct: 421 KIPTPNPWIRTEESEPPIVNFPQILNSDITTMVDRPE---EDRSGEEVDEVLVLEDVEYD 480

Query: 486 KRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIE 545
               VHF+VLVNVVD   D C    EFVG+F  + HG +H  KTSLR VITEV++ LG+E
Sbjct: 481 PTTRVHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLE 540

Query: 546 DDESVIVRLDPKVGGDKVTIGGMKIELQP 567
            D+++ V+++P VGG+ VT+GG+KI+L+P
Sbjct: 541 RDKNLAVKIEPMVGGEGVTVGGIKIQLEP 556

BLAST of CmaCh18G003560 vs. NCBI nr
Match: XP_022994369.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 125
           AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ
Sbjct: 61  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 305
           QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIELQPKGQPKGQ 574
           LDPKVGGDKVTIGGMKIELQPKGQPKGQ
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGQPKGQ 568

BLAST of CmaCh18G003560 vs. NCBI nr
Match: KAG7012418.1 (hypothetical protein SDJN02_25170, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 544/569 (95.61%), Postives = 554/569 (97.36%), Query Frame = 0

Query: 1   MPLKTMASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGL 60
           MPLKTMASISPLLSILPYSQIKNQTSN QNKSRGINHVPCRASNGDSTSKFDRRDVLFGL
Sbjct: 1   MPLKTMASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGL 60

Query: 61  GGLYGAAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTK 120
           GGLYGAAALTNQQSPASA PLAPDVNNCEPATI GN+HIPCCPPKIHDIIDFIHPPHTTK
Sbjct: 61  GGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTK 120

Query: 121 LRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAE 180
           LRIR PAHSLTPKQQFNFEKAIHIMKRL PNDPRNFYRQADVHCAYCDYAYKQLGFPNAE
Sbjct: 121 LRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAE 180

Query: 181 MQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLY 240
           MQVHWNALFFPFHRAYLYFFERILGHLINEP+F IPFWNWDN +GMQMPEIYDHPNSPLY
Sbjct: 181 MQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLY 240

Query: 241 DKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQE 300
           DKLRNQRHRPHKLLNLNYHGDDNP YDVVD NLRWMHKQMITDA+TAEGFHGKLL+AS E
Sbjct: 241 DKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHE 300

Query: 301 ANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQ 360
           ANEETGMGTIERSPHNNIHNWVGDPNAKN+MNMG FYSAARDPLFYGHHANVDRMWSIWQ
Sbjct: 301 ANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQ 360

Query: 361 GLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT 420
           GLKNGKPRDF+NPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT
Sbjct: 361 GLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT 420

Query: 421 PRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRY 480
           PRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRP KL +EKEKSEKDEVLVIEGIELDKR+
Sbjct: 421 PRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRF 480

Query: 481 WVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDE 540
           WVHFNVLVNVVDDGSDVCPGNTEFVGTFTTV HGHNHRGKTSLRVVITEVLKELGIEDDE
Sbjct: 481 WVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDE 540

Query: 541 SVIVRLDPKVGGDKVTIGGMKIELQPKGQ 570
           SVIVRLDPKVGGDKVTIGGMKIE QP+GQ
Sbjct: 541 SVIVRLDPKVGGDKVTIGGMKIEHQPRGQ 569

BLAST of CmaCh18G003560 vs. NCBI nr
Match: KAG6573251.1 (hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 538/563 (95.56%), Postives = 545/563 (96.80%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSN QNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 125
           AAALTNQQSPASA PLAPDVNNCEPATI GN+HIPCCPPKIH IIDFIHPPHTTKLRIR 
Sbjct: 61  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRP 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRL PNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEP+F IPFWNWDN +GMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 305
           QRHRPHKLLNLNYHGDDNP YDVVD NLRWMHKQMI DADTAEGF GKLLVAS EANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMIKDADTAEGFLGKLLVASDEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIERSPHNNIHNWVGDPNAKN+MNMG FYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           KPRDF+NPDWLNSSFVFYNEHKQAVRIKV DCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRP KL +EKEKSEKDEVLVIEGIELDKR+WVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTV HGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIELQPKG 569
           LDPKVGGDKVTIGGMKIELQPKG
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKG 563

BLAST of CmaCh18G003560 vs. NCBI nr
Match: XP_022954613.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1127.9 bits (2916), Expect = 0.0e+00
Identity = 537/564 (95.21%), Postives = 546/564 (96.81%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSN QNKSRGIN VPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINQVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 125
           AAALTNQQSPASA PLAPDVNNCE ATI GNEHIPCCPPKIHDIIDFIHPPHTTKLRIR 
Sbjct: 61  AAALTNQQSPASAAPLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRP 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRL PNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEP+F IPFWNWDN +GMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 305
           QRHRPHKLLNLNYHGDDNP YDVVD NLRWMHKQMITDA+TAEGFHGKLL+AS EANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIE+SPHNNIHNWVGDPNAKN+MNMG FYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIEKSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           K RDF+NPDWLNSSFVFYNEHKQAVRIKV DCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRP KL +EKEKSEKDEVLVIEGIELDKR+WVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTV HGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIELQPKGQ 570
           LDPKVGGDKVTIGGMKIELQPKGQ
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGQ 564

BLAST of CmaCh18G003560 vs. NCBI nr
Match: XP_023542623.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 531/564 (94.15%), Postives = 544/564 (96.45%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASI PLLS++P   IKNQTS  QNKSRGI+HVPCRASNG+STSKFDRRDVLFGLGGLYG
Sbjct: 1   MASIYPLLSVIP---IKNQTSKLQNKSRGISHVPCRASNGESTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 125
           AAALTNQQSPASA PLAPDVNNCEPATI GN+HIPCCPPKIH IIDFIHPPHTTKLRIR 
Sbjct: 61  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRP 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRL PNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEP+F IPFWNWDN +GMQMP+IYD+PNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNNEGMQMPKIYDNPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 305
            RHRPHKLLNLNYHGDDNP YDVVDCNLRWMHKQMITDADTAEGFHGKLL+AS EANEET
Sbjct: 241 PRHRPHKLLNLNYHGDDNPSYDVVDCNLRWMHKQMITDADTAEGFHGKLLIASHEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIERSPHNNIHNWVGDPNAKN+MNMG FYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           KPRDF+NPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 485
           QLK TQAPQVIKFPQGLNSMVTTTVKRP KLRDEKEKSEKDEVLVIEGIELDKRYWVHFN
Sbjct: 421 QLKATQAPQVIKFPQGLNSMVTTTVKRPNKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTV HGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIELQPKGQ 570
           LDPKVGGDKVTIGGMKIELQPKG+
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGR 561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P433092.6e-13848.17Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q6UIL32.6e-13043.64(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S... [more]
P433112.3e-12643.54Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Q9MB146.6e-12645.08Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=... [more]
Q062152.5e-12543.56Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K2N90.0e+00100.00polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114901... [more]
A0A6J1GRK50.0e+0095.21polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A172DC013.5e-19460.31Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1[more]
A0A5A7UTU03.4e-15754.31Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861... [more]
A0A0A0LUI15.8e-15752.90Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022994369.10.0e+00100.00polyphenol oxidase, chloroplastic-like [Cucurbita maxima][more]
KAG7012418.10.0e+0095.61hypothetical protein SDJN02_25170, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6573251.10.0e+0095.56hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022954613.10.0e+0095.21polyphenol oxidase, chloroplastic-like [Cucurbita moschata][more]
XP_023542623.10.0e+0094.15polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 215..220
score: 57.82
coord: 314..325
score: 39.97
coord: 341..359
score: 57.04
coord: 184..201
score: 51.36
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 153..360
e-value: 3.2E-33
score: 115.9
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 184..201
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 342..353
IPR016213Polyphenol oxidasePIRSFPIRSF000290PPO_plantcoord: 2..566
e-value: 2.3E-211
score: 701.6
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 438..564
e-value: 6.0E-39
score: 133.1
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 80..416
e-value: 6.4E-105
score: 353.3
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 97..415
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 369..418
e-value: 9.5E-23
score: 79.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..47
NoneNo IPR availablePANTHERPTHR11474:SF87POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 32..563
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 32..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G003560.1CmaCh18G003560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046148 pigment biosynthetic process
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity