CmaCh18G002710 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G002710
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionaspartic proteinase-like
LocationCma_Chr18: 1408668 .. 1419683 (-)
RNA-Seq ExpressionCmaCh18G002710
SyntenyCmaCh18G002710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACCCTTTACACGCAGGCCTTCTACTTCTTCCTCCACTAAATCAACCCCTGGCCGTCACGACGGCAAGGCTGTTCGGTGGTCGGAGCCGGTCACCGGCGTTGACTCTGCTGCAGTAAAGGACCACAATGGCGTCAACAACGATGAAGACGAAGACTTTGAGGTCGGTGAAGATGAGTCACCGGCGCGTGGGGATGAAGAAAATCCGCTACCGCTTGAGATTTACGGCAAACCGTTAGATCCCAAGACTAAAGATCTGAGCTGGCCTATGTCCAAATTTCACAGCTTCAGATTTTCCAGTAAGTTTTTACTCTTCCCATTTTAACCTCTAATTTACTCTAATTTACCTTTTAGTCCTTAATTTAGGATCTATAAATAAATAAATAAATAAATAAATAAATAAATAAATAATAATAATAATAAATTTATTTTGTCCCTGCCCATGCCCCTAGTACGTGTACCAATAAATGTCATTTCTGATATTAGGTAGGTATTAAAATCAATAATATCAATAAAATTTATTCAATTTTGGGTATTGTTTTTATTTTTGTTTCTTATTTTAAAATATAATCATTTAAGATATTTATTTTTTATATTATCAATTATTTATTCATATATATGTTGATATTGAAATGTATTGATCTACCTAGACTAGTCAACTCATGACCTTACTTGATTGGTCACGATATAAAAAGTTTATCTACTTTTATTTATTTATTTATTTATATATATATATATATATATATATATTTTAAACATTAATTCAATTTTTGAAAACTAAAACAAATTATATATATATTTTTGAATTAGTCTTTTAATAGGTGAAAAATAAGTATAATTTTCAAAAAAATATTTGATTTATTTATCATATTTACTGTCTTATTTACCTCAAATGTCTTCAATAAAAAAAATAAAATAATAATAGCTTAAGATAGTGAATATTTGCAAGAAGAGGGTTAGAATGATGTTAATGGACTCGGATGGTCAAAAATTTATACGATCTCTAGGAAAGAAGCCAACATCATATATGAAGCGTAAAGGAGTTACTCCACTTCAAAGAGAGTAGTTGATTAGAACATAGATGATTTAGATGGGCCACATGAGAAGAAGAAGAGGCATAAGAGGGATTACTCGTGGTCAAATTTGCGTAGTTGGCCAATTGGACGTTTTCAAAAGTATACTCCAAAGACAAGTAATGAAATAAGTTGTGTTTATGGGTATAATAATTACATGCAAGTCGATCATTTACAAGTTATTTGTAGGTACAATTGAATTAAAAGTCTATTTTATCCCTATAATTATATGTAAGGTGTCATTCATGTTTGATTAACAATATGTTTATGATCCTAGTATAAATTAAGTCTCTCAATGTAGTAAGAAGGTGAACACCCATGGTTCTAGTTTTAAAATAAGTAAGGAAACTACTCATCTACCTTTCTACCCTCCCTAGAAGTGAATTTCATCTCGTGAAATTATGTTATGAAATGATAGGCTTACTGAATCAGCTACAAAAGCTAATCCCACTCATGCAAATCAAAAAACAAAACCCAACATGCAGAAGTTCGTAACTTACTAAAATTAAGATCCAAATTTAGATTTATAAAGAAATTGTTATCAAGATAAGACACTCAACCTTATCGGTTTAGTATGGATCTTTTAGACTATTTCTTAAACATGATCGTCTTATATGTCAACAAAAGATTATTCAATGAATCATGAGACGCCTAGTTCATCCTTCCGTCAATATAATTTGAGAAAGGAAAAAGAACCCTTAAATCGTCCTCATATAATACCTCGTTAAAATTTAATTAGTGATAACTTGGATTCAATGTTCAAACTCTTTAACTTGTTACACTTTGACCCGTTACACTTTTCAGAGAGTAGAAGAATGGACAATCGTCCACTGCGTGTTCAACTATACCAAGCTGCACAAAATGGCGACTGGAAGACTGCTCAGTACATGAATATTCTGTATCCAGGAGTCCTGACTATGGTCATAAGCGACCGATGCGAAACTGTTCTTCACATAGCAACTCGAGCCAAGAAGGCTTTTTTTGTGAAGGAGTTGGTGAACTTCCTCGACCGACATGACTTGGGCTTGAAAAACAAATATGGAAACACAGCCCTTTGCATCGCTGCAGCCTCAGGCGCAGTCGATATCGCCAAGCTAATGGTTTCCAAGTTTGAAGCTTTGCCGCTCATTCGTGGGTCCGGAAATTCAACCCCAGTTTTGATCGCCGCTAGATACAAACACAAGCACATGGTCTCCTATCTCCTCTCCAAGACCCCCGTCTATGGCTCCGCAATTCAAGAGCAAATGGAGCTTCTCATTGGCGCCATCTCGGCAGATTATTATGGTTTGTTTTAAATTTTTGTTAAGAATCACGACTCTCTATGGTATGATATTGTCCACTTTGAACATGAGCTTTTATGACTTTGCTTTGGGCTTCTCCAAAAGACCTCATACCAATAGAGGGAGTATTATTTGATTAACAAAGTACGTCTTTCATTAATCGAGACTCGACTCTTTTTTTCTTTTGGAGTCCTTTGCTCGAAATTTGAGGAGATTCTATTGGCATGGCTAAGTTTAGGGCATGACTCTGATACCATGTTAGAAATCACGACTCTCCACAATGGTATGATATTGTCCACTTCGAGTATAAGCTCTCATGGCTTTGCTTTGGGTTTCCCCAAAAGGGCTCATATCAATGGAGAGAGTATTCTTTGATTATACTTTCATCCAACAATGATATTGTCCACCTTTGAGTATAAGCTCGGGTGACTTTGCTTTTGGGCTTCCCAAAAGGCATCATACCAATGGAAATGTATTCTAAATCCATGATCATTTTAAATTAGCCAACGTGGGACAATCTTTAACAACTTCATGATTGCTTTCTTCTACAGATATAGCTTTGCTTATTTTAAAGTGGAACCCGTTGTTAGTTCTCGAGCGAGACTTCAATGACGATACGCCCTTGCATATCATGGCTCGTAGGTCGAATGCAATTGGTAAGAAAAACAAACCAACCAAGTGGCAATCATACATTATTGATTGTGAAGAACAAACATCTAATCTCTTAGTCAAAGGTATATATTTTAATCGCTTGTGTTTTTTTTTTTTTTTAGCTATTCGTTTTTTAATTTAATCTATCTTTAGGAATCAAGCGTATGCACAAACATAAACTCATGCAGATTCAAGCTCATCAAATGGTTGAATTGATGTGGAGTGTTGTTTTGGATGAGATTCCAGAAGATGAGATATTGCAGTTCATCATGTTTCCCACGAGCATCTTGCACGATGCTGCTAGAGTTGGGAATGTTGAATTTTTGAGATTGATAATCAATTCATACCCTGATCTTGCTTGGAAAGTTGATAGCGATCGAAAGAGTATATTTCATGTAGCGGTTGAAAATCGACAAGAGAGTGTTTTTAGTTTAATTTATGAAATGGGTGAGTTCTTGGATTACTTACCATTCTATTTTGATGAGGAAAATATCAGCTTGCTTGAACTAGCGGCGAAAAAGGCGGATCTAAATCATCTCAATCGAGTGTCGGGAGCTGCCTTTCAAATGCATAAAGAGCTTCTGTGGTTTAAGGTATCGTGAGTTTATAAGTTATGAATGTCATTTCTGTGTTGGTTAAGGCTTTGTGGGAAACCAAAAACAAAATCATGTGAGTTTACTATACACACTCTTATAAATAATATTTCGTTATCCTCTCCCACCGATGTGGGATCTCACAACAACCCTCCTTCCGGGCCCAACGCCCTCACTGACACACTGCTCGATATCTAACTTTGACACCATTTGTAACGGCCTAAACCTACCACTAGCAGATATTGTCCTCTTTGAACTTCCCTTTTCGAACTTCCCCTCAAGGTTTTTAGAACACATCTCCTAGAGAGAGGTTTTCACACTCTTCTAAAAAAATGTTTCATTCTTCTCCCCAACCGATGTGAGATCTCACAATCCACCTTCCTTCGGGGCCCAGCGTTCTCACTGGCACTCGTTCCCTTCTCCAATCGATGTAGGACCTCCCAATCCACCTCCTTCAGAGCCCAGCATCCTTGCTGGCACACCACCTCATGTCCATCCCCTTCAGGGTTCAACCTCCTTGCAATGTTAGGTTAGCAGAAATCTAGCATGTGTTTCTACCAACGGTGAGTTGGAGCTATTTCAATTACACTCAAAATGAATAATATCATATCATTATTGGAAGTTCATAATTCCTAACAAGTAGCTTTTCAAAATGGTTAGTAGTGATTCAATACATATGTTTCTAAACTAAACTATGCAATTGAAGGAAGTGGAGAAGATCGTAGAGCTTACAATGAGGAGAAAGAAAGGAAAGCGAAACCCACGTGAATTATTCACCAAAGAACACCGAAACTTAGTGGAAGAAGGAGAAAAATGGATGAAGAAAACAGCAAATTCATGCATGTTGGTTGCAACTCTAATTGCCACCGTTGTTTTTGCTGCAATTTTCACCGTACCAGGCGGCAACAACAACAACCACGACATCAACACCGGCTCTCCTCTCTTTCTCCGCCACAAATGGTTCACAGTGTTCGTGATATCAGATGCAACAGCTTTGATATCATCTTCAACATCAATACTATTGTTTTTGTCGATCCTCACGTCGCGTTGTGCCGAAGAAGACTTCCTAATTTGGTTGCCATTAAAGTTGGTGTGCGGACTTGGAACACTGTTCTTGTCAGTACTGAGCATGGTGCTAGCTTTCAGTGCTACGTTCTTCCTGTTCTATGGGAAAGACACGGATTGGGTTCCTTTGCTTGTTGCTGGGATGGCGATTGTTCCAGTTTATTGTTTTGGTGTGCTACAGTTTAGGCTTTGGGCTGATGCGTTAGCAGCTTTGCAGGCTTCTTATTACTTGTATTTCAAGAATTGGAAGTTCATATTGTTCTGATCTCTCAATCATTTGTTCTTTCCGTAAAATCTTCCCATAAAGGTGGAACTTGAGTATTTATATGAAAACCCTTTTAGCGTTTTTGTGTTCTCTTTGCAAATTAGGTTAAAGAGTGAACAAAACCGAATCGAATAGAGGTCGATTTCCTTTCTCCCACCTTTGTGGTTTGATATGTTATTGTGATATCCCATATTGGTTGGGGAAGAGAACGAAACACTCTTTATAATGGTATGGAAACTTTTCCGAGACGTTTTAAAGATTTTGAGAAGAAGCCGGAAGGGAAAACCCAAATAAGACAATATCTGGTAGCGGTGGGCTAAAGGGTGTAGAAAGTCCAAATGGGATAATATCTGCTAGTGGTCGGGCAAACCCAAATGGGACAATATCGACTAGTGGTCAGACAAACCCAAATGAGACAATATCTGCTAGTGGTCGAGCAAACACAAATAGGATAATATCTACTAGTGGGGGGTCTGAGCTGTTATATTTCCCTAGCAGACCCGTTTTAAAGCTTGAGTAAAAGCCCAAAGAGGACTGAGCTTTTATATTTATGTTAACTCAAATTTGTCTCGTACTATATCTTTTTCTATTAACTTGGTGTCAGAAGAGTACATCGATTGAAGAGGAGAACAAAATATTGTTTCTTTTCTATAATATACAAGGTAAACGCGGAAGGGAAAGCCCAAAAAAGACAATATTTGCTATGGGTGGACTTGCTGTTACAAATAGTTTCAGAACCAGATAATGAGCGGTGTGCCAACGAAGACGTTGGACCCTTGAGAGAGGTGAATTGTGAGACCTCAAATTGATTGAAGAAGAAAACGATAAACATTTCTTATAAGAGTAGAAACCTCTACATAACAAACGCGTTTTAAAATTATGAGACTGATGACGACAAGTAACAGGCCAATAGGTAACCTTAGAATTACACATCAAACCTCATAGGAATTGAATTTTCGAGTTATTTTAAATAAATCTTACTTATGTTCACAAATAATAACTAGTAGAATTCAAATATCATATCATCAAAAGTAATAAACGTTAAATCATCTTAATTTTTCATAATAATTTGGAAGGTAAAATATGTTTGTTGGGTAAAAATGTCAACTCAGCTCATCCAATCAAATTTTAAATTTTAAAAAAATTTAAACTTTATTCGAGTTGGATGTTACCCTACCAAAAATGCTTTCTTTTCGGATCCCAAATTCGAATCAGAAACGTAGACGGCTCTACAGAAATCCCGGGAGGAAACACAACGTTGACTCCACACAACGGACGGCTCAGATCGAATCATTTCTTACGGTTGTTGAATTGGGTTTGCCTCATTTGTTTCTCCTTCGTTTATAAAGAGGTAGGGAGAGAAATTTGCGTGCCAACTCCCCAATTTTCTCCACCGTTTGAAATCCTTCGCTGTTGGTGCGTTTTCTCTTCGTTTGATTACAGATTTTTGTGCCATGATCCTTTTAGTTTTTGGGTTTTCATTTTTTGACTCCATTGTTTGCTTCATCGACAATTTGGTAACTGGGTTTTGTTTGTTCTTTTCATAATGTGATCATGATCTTGATGGGTTGTTCTGCTGATCATGTTTCCCTTTTGATCTTTAAGTTTTTGGATGCTTACGGGTCTGTAATCCCATTTGATCGTATGATATTTAGGGTTTGTTCTTTCTTTTGTCCTGCAATTTGGGGAAGTTTGAAATGTTGTGTTTCATTTTCTTGGATTGAATGATTATGTAGGAAATCTCATTGTTCATGATTTCTAAATTGGATCATCTCTTTAAACTTGTAGGTTACCAATGGCGTTGCCCCACTTCAAAGCGGCTTTCTTATGTTTGTTCTTGTTGGTTTCATTTAATATTGTATCATCTGCATCTGGGTTGCTTAGAGTTGGACTGAAGAAGATTAAATTAGACTCAAAAAGCCAGCTAGCAGCCCGGCTTCAGTCCAAGGATCCAGAGATTTTGAAAGCTACTTTCAGAAAGTCCAATAGTAATCTTGGACAATCTTCTGATACTGATATTGTTGTGTTAAAGAACTACATGGATGCTCAGTACTATGGTGAGATTGCCATTGGTACACCCCCACAAAAGTTCACTGTGATTTTCGACACCGGCAGCTCGAATCTATGGGTGCCTTCTGCAAAATGCTTGTTTTCTGTAAGGTTCTATCATGTTGATTATAACAGAAAGTAAATCATTCAAGTAGAAATGATATTTTTTGGAGTATACTATGATTATAGGCCACATACTCTCACATTTCTTTGTATGTTTTTGTGTAGTTGGCTTGTCATTTCCATGCCAAATACAAGTCGAGCCACTCTAGTTCATACAAGAAAAACGGTACTTCCCGATTTCCATAAGTCGGACTGAATTGTAATTATTAGTACTTATTTTGATGTGGGGATGTTTGTAGATTGTACTTGTGTGACGTGATATAGCAATTATACAATTGCAGTCCAAAATAATTCGGTTTATCGACTTTGTGCAGGGACATCTGCTTCGATACGGTATGGCACTGGAGCGGTCTCTGGTTTCTTTAGTAATGACAATGTCCGAGTTGGAGATCTAGTGGTGAAGAATCAGGTAACTTTTTGCATTAACTATTTTTCTATCGAATTAAATCGTTGGAATGTCGAGAATTAAATCGTTGGAATTTCGATGGTTCGATACTTATAGCTATTGTCTCGTTTAGGATTTCATTGAGGCAACCAGAGAACCAAGTCTTACGTTTCTTGTGGCCAAGTTTGACGGGTTGTTGGGACTTGGTTTTCAAGAGATCTCTGTTGGTAATGCTGTCCCAGTATGGTAGGACATTTTCATCCAATATTACTGATTATTGTTTAAGATTTCTTGGCTTGTAGAGGCTCGTATGAGGCGTTTGTAATTTTCTTCTCAGGTATAACATGGTTGATCAAGATCTTGTTAAGGAACCAGTCTTTTCGTTTTGGCTCAATCGCAACGTTGAGGAGGAAGGTGGTGAAATTGTGTTTGGTGGAGTCGACCCAAAGCACTATAAGGGCGAGCATACTTACGTTCCTGTCACACAGAAAGGTTATTGGCAGGTTGGTTGCCATGCCTTCATCTCGTATCTGCCCCGTTTATATCGTTCTTACTCACCATGTTTAAATTGTTCTGATTTGTAGTTTGACATGGGCGATGTTCTCATAGACGGTGAACCTACTGGTATGCTTCCATAAATCACATATTAATTATAATGTATCTGAAAAACTCGAATCTAAAATGATTTAGGCATGAATGAAGTTTTATTTTTTAATTCTTATATGGGGATTGAGTTTTACATTTACGTGCTTCTAATTAAAGAAAATTGTTATTTCAGGATATTGTGGTGGTGGTTGTTCAGCCATAGCTGATTCTGGAACTTCACTGTTGGCTGGTCCAACTGTGAGTACATAAACTTAACCTTTTCCTTCCTTTTCACCTGTAACTTCACTGGCTTCACATATGTTACCATGTTGCATAGTACTTTTTGAATCAATAAGTTGGAACTTTTCTTTGTTTCTTTGAAATGTGTAAGATCCCACATCAGTGGTAGAGGGGAACGAAACATTTCTTATAAGAGTGTGAAAACCTCCTCTCCCTAACAGACGTATTTTAAAACGTGAGGCTGACGACAATACGTAACAAGTCAAAGCAAACAATATCTGCTAGTGGTGGACTTGGACTTTTACAAATGATATTAGAGCTAGACACAAGGGGGTGTGTCAGCGAGGATGCTGGACCCCAAGGGGGTGTGTCAGCGAGGACGTTGGATCCCAAGGGGGTGGTGGTGGGTCAGCGAGGACGTTGGATCCCAAGGGGGTGGTGGTGGGTCAGCGAGGACGCTAGACCCCTAGGGTTGACGATGTGAAAACCTAACGGTGGTTGGAAAGGAGAATGAAACATTTCTTATAAGAGTGTGGAAACCTAACGGTGGACTTGGGCTGTTACAAATAGCATCAAAGATAGACACCAGGCTGTGTGCTAGCGAGGACGCTGGCCCCCAAAGGGGTGGATTATGAAATCTCATATCGGTTGGAGGGGGGTACGAAACATTTCTTTGAAGAGTGTGGAAACCTCTCCCTAACAAATGCGTTTTAAAACTGTGGTTGACGATGGTATGTAACAGGCCAAAGCGGATAATATCTGATAGCTGTGGATGGACTTGAGCGGTTACAAAATGTCTCAAGCATATGGAAGCTTGTTAACCTAAGATTTTGGGCTTTAACTTGGAGCGTGTGTGTAAATATATATATAATTCACTTCTTAGCCTTACGATTAAGTAGAACCGCTTTGACTTTGTCTTTTAAGTTGGGTCGAATAGCTACTTACTACAATGTTTGTCTATTTCTTATAGCCCATTATAACCATGATCAACCATGCCATTGGAGCTAAAGGAGTCATTAGTCAGGAATGCAAGGCAGTTGTTGCACAGTATGGGCAAACCATTATGGATTTGCTTTCATCCGAGGCAAGTTACTGTTATCCTTTTTTAACTCCCTGATTCTTAACATTATGATTTCTTTATAGACTCTGCTTAAGAGACCCAATATTTAAACCTTCTATACATTATTGACCTCTTGCTTTTTCGCTATCTTCAACAGGCAGATCCGAAGAAGATCTGTTCTCAAATTAAGTTGTGTACTTTCGATGGGGCCCGAGGAGTGAGGTGAATCCTTTTTTTTGGTACCACAATAGTCCTGCTATATGAAATCATCCTCACTAATTTACTTGGTGAATCGTTGATCCGTGAAATTATGTTGTTCGTGTAGCATGGGGATTGCGAGTGTGGTGGATGAGAAGGCTGGCAAATCATCTGATGGTCTACGCGATGCCATGTGCCCTGCATGTGAGATGATGGTCGTCTGGATGCAAAATCAACTTCGTCAGAATCAAACTAAAGAACGAATAATGAACTACATCAACGAGGTGAAAAAATCTCTTACTTGAAGAATGTTCCAGGTTCTTTAGAATATTTGTGTGATATCCCACATCGGTTGGAGAGAGGAACAAAGCATTGCTTATTATAAGGGTGTGGAAACCTCTCCGTATCGGACTGTTACAAATGGTATCAGAGTCAGACACCGGACGGTGTGCCAACGAGGACACTGGGCCCCCGAGAGAATGGATTGTGACATCCCACATTGGTCGGAGAGGGGAACGAAGCATTGCTTATAAGGGTGTGGAAACCTCTCCCTAACAGACATGTTTTAAAACTGTGAGGTTGACGGTGATACGTAACGGGTCAAAATAGACCATATCTGCCAGCGGTAGGCTTGGATTGTTATAATTTGGTTCTTGATGACTGAACTAGTTAACTCTTCTTGCAGCTATGTGATCATATGCCTAGTCCAATGGGACAATCTGCCGTTGACTGTGGAAGCCTTTCTTCCATGCCTATTGTTTCCTTCACCATTGGTGACAAAGTTTTTGACCTTACCCCACAAGAGGTAAAATACTTGTTTCTTTGATATTACACTTTTTTTTTACCAACCTTCTCTAACACGGTGAGCAATGTGAACAGTACGTCCTCAAGGTGGGCGAAGGTCGTGCAGCTCAGTGCATCAGTGGATTTACTGCGTTAGATGTTCCTCCTCCTCGTGGACCCCTCTGGTATAAGTTTTACCATTCATTTTGCTGTCAAAATCATTCAAAATAACCATTACCTTTCATTAAGAACCTGTTTTTGTTGGCCACAGGATCCTGGGAGATGTCTTCATGGGTCGATACCACACAGTGTTCGATTTTGGCAAGCTGAGAGTCGGGTTTGCAGAGGCAGCATGAAGAAAGAAACTTCTTGTTTGGTGGCTTTGTTTGGTATGCCTTTAAAGCTTATTATGCATATGAGCCATTTAAGTTGAAGTTTATGAACATATGGAATGTTAAGAAGGGAAACCCCCAAATTTGTAAATGCTTGCTACTGTGTCCTCTTTTATTGATACAGCTTGTACAGTAGCTTGAGTATCTCTGCTGATTTATATACTAACAGAAGCTGATATATATTTGGATATAAGTTGTGTTATCTCGATTCATAAAACAACCTTGAAAAACACAAACTTAGAAAAATTTGCTTCCAAAATCTCCCAGAAAAT

mRNA sequence

ATGAAACCCTTTACACGCAGGCCTTCTACTTCTTCCTCCACTAAATCAACCCCTGGCCGTCACGACGGCAAGGCTGTTCGGTGGTCGGAGCCGGTCACCGGCGTTGACTCTGCTGCAGTAAAGGACCACAATGGCGTCAACAACGATGAAGACGAAGACTTTGAGGTCGGTGAAGATGAGTCACCGGCGCGTGGGGATGAAGAAAATCCGCTACCGCTTGAGATTTACGGCAAACCGTTAGATCCCAAGACTAAAGATCTGAGCTGGCCTATGTCCAAATTTCACAGCTTCAGATTTTCCAAGAGTAGAAGAATGGACAATCGTCCACTGCGTGTTCAACTATACCAAGCTGCACAAAATGGCGACTGGAAGACTGCTCAGTACATGAATATTCTGTATCCAGGAGTCCTGACTATGGTCATAAGCGACCGATGCGAAACTGTTCTTCACATAGCAACTCGAGCCAAGAAGGCTTTTTTTGTGAAGGAGTTGGTGAACTTCCTCGACCGACATGACTTGGGCTTGAAAAACAAATATGGAAACACAGCCCTTTGCATCGCTGCAGCCTCAGGCGCAGTCGATATCGCCAAGCTAATGGTTTCCAAGTTTGAAGCTTTGCCGCTCATTCGTGGGTCCGGAAATTCAACCCCAGTTTTGATCGCCGCTAGATACAAACACAAGCACATGGTCTCCTATCTCCTCTCCAAGACCCCCGTCTATGGCTCCGCAATTCAAGAGCAAATGGAGCTTCTCATTGGCGCCATCTCGGCAGATTATTATGATATAGCTTTGCTTATTTTAAAGTGGAACCCGTTGTTAGTTCTCGAGCGAGACTTCAATGACGATACGCCCTTGCATATCATGGCTCGTAGGTCGAATGCAATTGGTAAGAAAAACAAACCAACCAAGTGGCAATCATACATTATTGATTGTGAAGAACAAACATCTAATCTCTTAGTCAAAGGAATCAAGCGTATGCACAAACATAAACTCATGCAGATTCAAGCTCATCAAATGGTTGAATTGATGTGGAGTGTTGTTTTGGATGAGATTCCAGAAGATGAGATATTGCAGTTCATCATGTTTCCCACGAGCATCTTGCACGATGCTGCTAGAGTTGGGAATGTTGAATTTTTGAGATTGATAATCAATTCATACCCTGATCTTGCTTGGAAAGTTGATAGCGATCGAAAGAGTATATTTCATGTAGCGGTTGAAAATCGACAAGAGAGTGTTTTTAGTTTAATTTATGAAATGGGTGAGTTCTTGGATTACTTACCATTCTATTTTGATGAGGAAAATATCAGCTTGCTTGAACTAGCGGCGAAAAAGGCGGATCTAAATCATCTCAATCGAGTGTCGGGAGCTGCCTTTCAAATGCATAAAGAGCTTCTGTGGTTTAAGGAAGTGGAGAAGATCGTAGAGCTTACAATGAGGAGAAAGAAAGGAAAGCGAAACCCACGTGAATTATTCACCAAAGAACACCGAAACTTAGTGGAAGAAGGAGAAAAATGGATGAAGAAAACAGCAAATTCATGCATGTTGGTTGCAACTCTAATTGCCACCGTTGTTTTTGCTGCAATTTTCACCGTACCAGGCGGCAACAACAACAACCACGACATCAACACCGGCTCTCCTCTCTTTCTCCGCCACAAATGGTTCACAGTGTTCGTGATATCAGATGCAACAGCTTTGATATCATCTTCAACATCAATACTATTGTTTTTGTCGATCCTCACGTCGCGTTGTGCCGAAGAAGACTTCCTAATTTGGTTGCCATTAAAGTTGGTGTGCGGACTTGGAACACTGTTCTTGTCAGTACTGAGCATGGTGCTAGCTTTCAGTGCTACGTTCTTCCTGTTCTATGGGAAAGACACGGATTGGGTTCCTTTGCTTGTTGCTGGGATGGCGATTGTTCCAGTTTATTGTTTTGGTGTGCTACAGTTTAGGCTTTGGGCTGATGCGTTAGCAGCTTTGCAGGCTTCTTATTACTTAAACGTAGACGGCTCTACAGAAATCCCGGGAGGAAACACAACGTTGACTCCACACAACGGACGGCTCAGATCGAATCATTTCTTACGGTTGTTGAATTGGGTTTGCCTCATTTGTTTCTCCTTCGTTTATAAAGAGGTAGGGAGAGAAATTTGCGTGCCAACTCCCCAATTTTCTCCACCGTTTGAAATCCTTCGCTGTTGGTTACCAATGGCGTTGCCCCACTTCAAAGCGGCTTTCTTATGTTTGTTCTTGTTGGTTTCATTTAATATTGTATCATCTGCATCTGGGTTGCTTAGAGTTGGACTGAAGAAGATTAAATTAGACTCAAAAAGCCAGCTAGCAGCCCGGCTTCAGTCCAAGGATCCAGAGATTTTGAAAGCTACTTTCAGAAAGTCCAATAGTAATCTTGGACAATCTTCTGATACTGATATTGTTGTGTTAAAGAACTACATGGATGCTCAGTACTATGGTGAGATTGCCATTGGTACACCCCCACAAAAGTTCACTGTGATTTTCGACACCGGCAGCTCGAATCTATGGGTGCCTTCTGCAAAATGCTTGTTTTCTTTGGCTTGTCATTTCCATGCCAAATACAAGTCGAGCCACTCTAGTTCATACAAGAAAAACGGGACATCTGCTTCGATACGGTATGGCACTGGAGCGGTCTCTGGTTTCTTTAGTAATGACAATGTCCGAGTTGGAGATCTAGTGGTGAAGAATCAGGATTTCATTGAGGCAACCAGAGAACCAAGTCTTACGTTTCTTGTGGCCAAGTTTGACGGGTTGTTGGGACTTGGTTTTCAAGAGATCTCTGTTGGTAATGCTGTCCCAGTATGGTATAACATGGTTGATCAAGATCTTGTTAAGGAACCAGTCTTTTCGTTTTGGCTCAATCGCAACGTTGAGGAGGAAGGTGGTGAAATTGTGTTTGGTGGAGTCGACCCAAAGCACTATAAGGGCGAGCATACTTACGTTCCTGTCACACAGAAAGGTTATTGGCAGTTTGACATGGGCGATGTTCTCATAGACGGTGAACCTACTGGATATTGTGGTGGTGGTTGTTCAGCCATAGCTGATTCTGGAACTTCACTGTTGGCTGGTCCAACTCCCATTATAACCATGATCAACCATGCCATTGGAGCTAAAGGAGTCATTAGTCAGGAATGCAAGGCAGTTGTTGCACAGTATGGGCAAACCATTATGGATTTGCTTTCATCCGAGGCAGATCCGAAGAAGATCTGTTCTCAAATTAAGTTGTGTACTTTCGATGGGGCCCGAGGAGTGAGCATGGGGATTGCGAGTGTGGTGGATGAGAAGGCTGGCAAATCATCTGATGGTCTACGCGATGCCATGTGCCCTGCATGTGAGATGATGGTCGTCTGGATGCAAAATCAACTTCGTCAGAATCAAACTAAAGAACGAATAATGAACTACATCAACGAGCTATGTGATCATATGCCTAGTCCAATGGGACAATCTGCCGTTGACTGTGGAAGCCTTTCTTCCATGCCTATTGTTTCCTTCACCATTGGTGACAAAGTTTTTGACCTTACCCCACAAGAGTACGTCCTCAAGGTGGGCGAAGGTCGTGCAGCTCAGTGCATCAGTGGATTTACTGCGTTAGATGTTCCTCCTCCTCGTGGACCCCTCTGGATCCTGGGAGATGTCTTCATGGGTCGATACCACACAGTGTTCGATTTTGGCAAGCTGAGAGTCGGGTTTGCAGAGGCAGCATGAAGAAAGAAACTTCTTGTTTGGTGGCTTTGTTTGGTATGCCTTTAAAGCTTATTATGCATATGAGCCATTTAAGTTGAAGTTTATGAACATATGGAATGTTAAGAAGGGAAACCCCCAAATTTGTAAATGCTTGCTACTGTGTCCTCTTTTATTGATACAGCTTGTACAGTAGCTTGAGTATCTCTGCTGATTTATATACTAACAGAAGCTGATATATATTTGGATATAAGTTGTGTTATCTCGATTCATAAAACAACCTTGAAAAACACAAACTTAGAAAAATTTGCTTCCAAAATCTCCCAGAAAAT

Coding sequence (CDS)

ATGAAACCCTTTACACGCAGGCCTTCTACTTCTTCCTCCACTAAATCAACCCCTGGCCGTCACGACGGCAAGGCTGTTCGGTGGTCGGAGCCGGTCACCGGCGTTGACTCTGCTGCAGTAAAGGACCACAATGGCGTCAACAACGATGAAGACGAAGACTTTGAGGTCGGTGAAGATGAGTCACCGGCGCGTGGGGATGAAGAAAATCCGCTACCGCTTGAGATTTACGGCAAACCGTTAGATCCCAAGACTAAAGATCTGAGCTGGCCTATGTCCAAATTTCACAGCTTCAGATTTTCCAAGAGTAGAAGAATGGACAATCGTCCACTGCGTGTTCAACTATACCAAGCTGCACAAAATGGCGACTGGAAGACTGCTCAGTACATGAATATTCTGTATCCAGGAGTCCTGACTATGGTCATAAGCGACCGATGCGAAACTGTTCTTCACATAGCAACTCGAGCCAAGAAGGCTTTTTTTGTGAAGGAGTTGGTGAACTTCCTCGACCGACATGACTTGGGCTTGAAAAACAAATATGGAAACACAGCCCTTTGCATCGCTGCAGCCTCAGGCGCAGTCGATATCGCCAAGCTAATGGTTTCCAAGTTTGAAGCTTTGCCGCTCATTCGTGGGTCCGGAAATTCAACCCCAGTTTTGATCGCCGCTAGATACAAACACAAGCACATGGTCTCCTATCTCCTCTCCAAGACCCCCGTCTATGGCTCCGCAATTCAAGAGCAAATGGAGCTTCTCATTGGCGCCATCTCGGCAGATTATTATGATATAGCTTTGCTTATTTTAAAGTGGAACCCGTTGTTAGTTCTCGAGCGAGACTTCAATGACGATACGCCCTTGCATATCATGGCTCGTAGGTCGAATGCAATTGGTAAGAAAAACAAACCAACCAAGTGGCAATCATACATTATTGATTGTGAAGAACAAACATCTAATCTCTTAGTCAAAGGAATCAAGCGTATGCACAAACATAAACTCATGCAGATTCAAGCTCATCAAATGGTTGAATTGATGTGGAGTGTTGTTTTGGATGAGATTCCAGAAGATGAGATATTGCAGTTCATCATGTTTCCCACGAGCATCTTGCACGATGCTGCTAGAGTTGGGAATGTTGAATTTTTGAGATTGATAATCAATTCATACCCTGATCTTGCTTGGAAAGTTGATAGCGATCGAAAGAGTATATTTCATGTAGCGGTTGAAAATCGACAAGAGAGTGTTTTTAGTTTAATTTATGAAATGGGTGAGTTCTTGGATTACTTACCATTCTATTTTGATGAGGAAAATATCAGCTTGCTTGAACTAGCGGCGAAAAAGGCGGATCTAAATCATCTCAATCGAGTGTCGGGAGCTGCCTTTCAAATGCATAAAGAGCTTCTGTGGTTTAAGGAAGTGGAGAAGATCGTAGAGCTTACAATGAGGAGAAAGAAAGGAAAGCGAAACCCACGTGAATTATTCACCAAAGAACACCGAAACTTAGTGGAAGAAGGAGAAAAATGGATGAAGAAAACAGCAAATTCATGCATGTTGGTTGCAACTCTAATTGCCACCGTTGTTTTTGCTGCAATTTTCACCGTACCAGGCGGCAACAACAACAACCACGACATCAACACCGGCTCTCCTCTCTTTCTCCGCCACAAATGGTTCACAGTGTTCGTGATATCAGATGCAACAGCTTTGATATCATCTTCAACATCAATACTATTGTTTTTGTCGATCCTCACGTCGCGTTGTGCCGAAGAAGACTTCCTAATTTGGTTGCCATTAAAGTTGGTGTGCGGACTTGGAACACTGTTCTTGTCAGTACTGAGCATGGTGCTAGCTTTCAGTGCTACGTTCTTCCTGTTCTATGGGAAAGACACGGATTGGGTTCCTTTGCTTGTTGCTGGGATGGCGATTGTTCCAGTTTATTGTTTTGGTGTGCTACAGTTTAGGCTTTGGGCTGATGCGTTAGCAGCTTTGCAGGCTTCTTATTACTTAAACGTAGACGGCTCTACAGAAATCCCGGGAGGAAACACAACGTTGACTCCACACAACGGACGGCTCAGATCGAATCATTTCTTACGGTTGTTGAATTGGGTTTGCCTCATTTGTTTCTCCTTCGTTTATAAAGAGGTAGGGAGAGAAATTTGCGTGCCAACTCCCCAATTTTCTCCACCGTTTGAAATCCTTCGCTGTTGGTTACCAATGGCGTTGCCCCACTTCAAAGCGGCTTTCTTATGTTTGTTCTTGTTGGTTTCATTTAATATTGTATCATCTGCATCTGGGTTGCTTAGAGTTGGACTGAAGAAGATTAAATTAGACTCAAAAAGCCAGCTAGCAGCCCGGCTTCAGTCCAAGGATCCAGAGATTTTGAAAGCTACTTTCAGAAAGTCCAATAGTAATCTTGGACAATCTTCTGATACTGATATTGTTGTGTTAAAGAACTACATGGATGCTCAGTACTATGGTGAGATTGCCATTGGTACACCCCCACAAAAGTTCACTGTGATTTTCGACACCGGCAGCTCGAATCTATGGGTGCCTTCTGCAAAATGCTTGTTTTCTTTGGCTTGTCATTTCCATGCCAAATACAAGTCGAGCCACTCTAGTTCATACAAGAAAAACGGGACATCTGCTTCGATACGGTATGGCACTGGAGCGGTCTCTGGTTTCTTTAGTAATGACAATGTCCGAGTTGGAGATCTAGTGGTGAAGAATCAGGATTTCATTGAGGCAACCAGAGAACCAAGTCTTACGTTTCTTGTGGCCAAGTTTGACGGGTTGTTGGGACTTGGTTTTCAAGAGATCTCTGTTGGTAATGCTGTCCCAGTATGGTATAACATGGTTGATCAAGATCTTGTTAAGGAACCAGTCTTTTCGTTTTGGCTCAATCGCAACGTTGAGGAGGAAGGTGGTGAAATTGTGTTTGGTGGAGTCGACCCAAAGCACTATAAGGGCGAGCATACTTACGTTCCTGTCACACAGAAAGGTTATTGGCAGTTTGACATGGGCGATGTTCTCATAGACGGTGAACCTACTGGATATTGTGGTGGTGGTTGTTCAGCCATAGCTGATTCTGGAACTTCACTGTTGGCTGGTCCAACTCCCATTATAACCATGATCAACCATGCCATTGGAGCTAAAGGAGTCATTAGTCAGGAATGCAAGGCAGTTGTTGCACAGTATGGGCAAACCATTATGGATTTGCTTTCATCCGAGGCAGATCCGAAGAAGATCTGTTCTCAAATTAAGTTGTGTACTTTCGATGGGGCCCGAGGAGTGAGCATGGGGATTGCGAGTGTGGTGGATGAGAAGGCTGGCAAATCATCTGATGGTCTACGCGATGCCATGTGCCCTGCATGTGAGATGATGGTCGTCTGGATGCAAAATCAACTTCGTCAGAATCAAACTAAAGAACGAATAATGAACTACATCAACGAGCTATGTGATCATATGCCTAGTCCAATGGGACAATCTGCCGTTGACTGTGGAAGCCTTTCTTCCATGCCTATTGTTTCCTTCACCATTGGTGACAAAGTTTTTGACCTTACCCCACAAGAGTACGTCCTCAAGGTGGGCGAAGGTCGTGCAGCTCAGTGCATCAGTGGATTTACTGCGTTAGATGTTCCTCCTCCTCGTGGACCCCTCTGGATCCTGGGAGATGTCTTCATGGGTCGATACCACACAGTGTTCGATTTTGGCAAGCTGAGAGTCGGGTTTGCAGAGGCAGCATGA

Protein sequence

MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDEDEDFEVGEDESPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQNGDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKYGNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPVYGSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKNKPTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQFIMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEMGEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMRRKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNHDINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCGLGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAALQASYYLNVDGSTEIPGGNTTLTPHNGRLRSNHFLRLLNWVCLICFSFVYKEVGREICVPTPQFSPPFEILRCWLPMALPHFKAAFLCLFLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATFRKSNSNLGQSSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATREPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEEEGGEIVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVSMGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Homology
BLAST of CmaCh18G002710 vs. ExPASy Swiss-Prot
Match: O04057 (Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 1.9e-258
Identity = 447/514 (86.96%), Postives = 471/514 (91.63%), Query Frame = 0

Query: 735  MALPHFKAAFLCLFLLVSFNIVSSAS--GLLRVGLKKIKLDSKSQLAARLQSKDPEILKA 794
            MA  H KAAFLCLFLLVSFNIVSSAS  GLLRVGLKKIKLD +++LAAR++SKD EILKA
Sbjct: 1    MASYHSKAAFLCLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKA 60

Query: 795  TFRKSN--SNLGQSSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAK 854
             FRK N   NLG+SSDTDIV LKNY+DAQYYGEIAIGTPPQKFTVIFDTGSSNLWV   +
Sbjct: 61   AFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWV-LCE 120

Query: 855  CLFSLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIE 914
            CLFS+ACHFHA+YKSS SSSYKKNGTSASIRYGTGAVSGFFS DNV+VGDLVVK Q FIE
Sbjct: 121  CLFSVACHFHARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIE 180

Query: 915  ATREPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNV-EEE 974
            ATREPSLTFLVAKFDGLLGLGFQEI+VGNAVPVWYNMV+Q LVKEPVFSFWLNRNV EEE
Sbjct: 181  ATREPSLTFLVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEE 240

Query: 975  GGEIVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSL 1034
            GGEIVFGGVDPKHY+G+HTYVPVTQKGYWQFDMGDVLIDGEPTG+C GGCSAIADSGTSL
Sbjct: 241  GGEIVFGGVDPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSL 300

Query: 1035 LAGPTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDG 1094
            LAGPTP+ITMINHAIGAKGV+SQ+CKAVVAQYGQTIMDLL SEADPKKICSQI LCTFDG
Sbjct: 301  LAGPTPVITMINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDG 360

Query: 1095 ARGVSMGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCD 1154
             RGVSMGI SVVDE AGKSSD L D MC  CEM VVWMQNQLRQNQTKERI+NYINELCD
Sbjct: 361  TRGVSMGIESVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCD 420

Query: 1155 HMPSPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVP 1214
             MPSPMGQSAVDCG LSSMP VSFTIG K+FDL P+EY+LKVGEG  AQCISGFTA D+P
Sbjct: 421  RMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIP 480

Query: 1215 PPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 1244
            PPRGPLWILGDVFMGRYHTVFDFGKLRVG AEAA
Sbjct: 481  PPRGPLWILGDVFMGRYHTVFDFGKLRVGSAEAA 513

BLAST of CmaCh18G002710 vs. ExPASy Swiss-Prot
Match: O65390 (Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 3.8e-219
Identity = 374/501 (74.65%), Postives = 424/501 (84.63%), Query Frame = 0

Query: 749  LLVSFNIVSSA-----SGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATFRKSNSNLGQ 808
            L+VSF +  SA      G  RVGLKK+KLDSK++LAAR++SK  + L+A        LG 
Sbjct: 12   LIVSFLLCFSAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRA------YRLGD 71

Query: 809  SSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSLACHFHAKY 868
            S D D+VVLKNY+DAQYYGEIAIGTPPQKFTV+FDTGSSNLWVPS+KC FSLAC  H KY
Sbjct: 72   SGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSLACLLHPKY 131

Query: 869  KSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATREPSLTFLVAK 928
            KSS SS+Y+KNG +A+I YGTGA++GFFSND V VGDLVVK+Q+FIEAT+EP +TF+VAK
Sbjct: 132  KSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEPGITFVVAK 191

Query: 929  FDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNV-EEEGGEIVFGGVDPKH 988
            FDG+LGLGFQEISVG A PVWYNM+ Q L+KEPVFSFWLNRN  EEEGGE+VFGGVDP H
Sbjct: 192  FDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELVFGGVDPNH 251

Query: 989  YKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPTPIITMINH 1048
            +KG+HTYVPVTQKGYWQFDMGDVLI G PTG+C  GCSAIADSGTSLLAGPT IITMINH
Sbjct: 252  FKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTTIITMINH 311

Query: 1049 AIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVSMGIASVVD 1108
            AIGA GV+SQ+CK VV QYGQTI+DLL SE  PKKICSQI LCTFDG RGVSMGI SVVD
Sbjct: 312  AIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVSMGIESVVD 371

Query: 1109 EKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSPMGQSAVDC 1168
            ++  K S+G+ DA C ACEM VVW+Q+QLRQN T+ERI+NY+NELC+ +PSPMG+SAVDC
Sbjct: 372  KENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMGESAVDC 431

Query: 1169 GSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGPLWILGDVF 1228
              LS+MP VS TIG KVFDL P+EYVLKVGEG  AQCISGF ALDV PPRGPLWILGDVF
Sbjct: 432  AQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLWILGDVF 491

Query: 1229 MGRYHTVFDFGKLRVGFAEAA 1244
            MG+YHTVFDFG  +VGFAEAA
Sbjct: 492  MGKYHTVFDFGNEQVGFAEAA 506

BLAST of CmaCh18G002710 vs. ExPASy Swiss-Prot
Match: Q8VYL3 (Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.0e-216
Identity = 366/497 (73.64%), Postives = 416/497 (83.70%), Query Frame = 0

Query: 748  FLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATFRKSNSNL-GQSSD 807
            FLL          G  RVGLKK+KLD  ++LA R  SK  E L+++ R  N+NL G S D
Sbjct: 16   FLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRSSLRSYNNNLGGDSGD 75

Query: 808  TDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSLACHFHAKYKSS 867
             DIV LKNY+DAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPS KC FSL+C+FHAKYKSS
Sbjct: 76   ADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKCFFSLSCYFHAKYKSS 135

Query: 868  HSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATREPSLTFLVAKFDG 927
             SS+YKK+G  A+I YG+G++SGFFS D V VGDLVVK+Q+FIE T EP LTFLVAKFDG
Sbjct: 136  RSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIETTSEPGLTFLVAKFDG 195

Query: 928  LLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVE-EEGGEIVFGGVDPKHYKG 987
            LLGLGFQEI+VGNA PVWYNM+ Q L+K PVFSFWLNR+ + EEGGEIVFGGVDPKH++G
Sbjct: 196  LLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSEEGGEIVFGGVDPKHFRG 255

Query: 988  EHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPTPIITMINHAIG 1047
            EHT+VPVTQ+GYWQFDMG+VLI GE TGYCG GCSAIADSGTSLLAGPT ++ MIN AIG
Sbjct: 256  EHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGPTAVVAMINKAIG 315

Query: 1048 AKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVSMGIASVVDEKA 1107
            A GV+SQ+CK VV QYGQTI+DLL +E  PKKICSQI LC +DG  GVSMGI SVVD++ 
Sbjct: 316  ASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYDGTHGVSMGIESVVDKEN 375

Query: 1108 GKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSPMGQSAVDCGSL 1167
             +SS GLRDA CPACEM VVW+Q+QLRQN T+ERI+NYINE+C+ MPSP G+SAVDC  L
Sbjct: 376  TRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAVDCSQL 435

Query: 1168 SSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGPLWILGDVFMGR 1227
            S MP VSFTIG KVFDL P+EYVLK+GEG  AQCISGFTALD+PPPRGPLWILGDVFMG+
Sbjct: 436  SKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGK 495

Query: 1228 YHTVFDFGKLRVGFAEA 1243
            YHTVFDFG  +VGFAEA
Sbjct: 496  YHTVFDFGNEQVGFAEA 512

BLAST of CmaCh18G002710 vs. ExPASy Swiss-Prot
Match: Q42456 (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 716.5 bits (1848), Expect = 5.4e-205
Identity = 347/500 (69.40%), Postives = 413/500 (82.60%), Query Frame = 0

Query: 745  LCLFLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATFRKSNSNLGQS 804
            L   LL +    S+A GL+R+ LKK  +D  S++AARL S +    +   R +NS  G  
Sbjct: 11   LAAVLLQALLPASAAEGLVRIALKKRPIDENSRVAARL-SGEEGARRLGLRGANSLGGGG 70

Query: 805  SDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSLACHFHAKYK 864
             + DIV LKNYM+AQY+GEI +GTPPQKFTVIFDTGSSNLWVPSAKC FS+AC FH++YK
Sbjct: 71   GEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIACFFHSRYK 130

Query: 865  SSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATREPSLTFLVAKF 924
            S  SS+Y+KNG  A+I+YGTG+++GFFS D+V VGDLVVK+Q+FIEAT+EP LTF+VAKF
Sbjct: 131  SGQSSTYQKNGKPAAIQYGTGSIAGFFSEDSVTVGDLVVKDQEFIEATKEPGLTFMVAKF 190

Query: 925  DGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEE-EGGEIVFGGVDPKHY 984
            DG+LGLGFQEISVG+AVPVWY MV+Q LV EPVFSFW NR+ +E EGGEIVFGG+DP HY
Sbjct: 191  DGILGLGFQEISVGDAVPVWYKMVEQGLVSEPVFSFWFNRHSDEGEGGEIVFGGMDPSHY 250

Query: 985  KGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPTPIITMINHA 1044
            KG HTYVPV+QKGYWQF+MGDVLI G+ TG+C  GCSAIADSGTSLLAGPT IIT IN  
Sbjct: 251  KGNHTYVPVSQKGYWQFEMGDVLIGGKTTGFCASGCSAIADSGTSLLAGPTAIITEINEK 310

Query: 1045 IGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVSMGIASVVDE 1104
            IGA GV+SQECK VV+QYGQ I+DLL +E  P KICSQ+ LCTFDG  GVS GI SVVD+
Sbjct: 311  IGATGVVSQECKTVVSQYGQQILDLLLAETQPSKICSQVGLCTFDGKHGVSAGIKSVVDD 370

Query: 1105 KAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSPMGQSAVDCG 1164
            +AG+S+      MC ACEM VVWMQNQL QN+T++ I+NYIN+LCD +PSPMG+S+VDCG
Sbjct: 371  EAGESNGLQSGPMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPMGESSVDCG 430

Query: 1165 SLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGPLWILGDVFM 1224
            SL+SMP +SFTIG K F L P+EY+LKVGEG AAQCISGFTA+D+PPPRGPLWILGDVFM
Sbjct: 431  SLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFM 490

Query: 1225 GRYHTVFDFGKLRVGFAEAA 1244
            G YHTVFD+GK+RVGFA++A
Sbjct: 491  GAYHTVFDYGKMRVGFAKSA 509

BLAST of CmaCh18G002710 vs. ExPASy Swiss-Prot
Match: P42210 (Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.1e-202
Identity = 338/498 (67.87%), Postives = 408/498 (81.93%), Query Frame = 0

Query: 747  LFLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATFRKSNSNLGQSSD 806
            L L       S A GL+R+ LKK  +D  S++A  L   + + L +      + L    +
Sbjct: 15   LLLQTVLPAASEAEGLVRIALKKRPIDRNSRVATGLSGGEEQPLLS----GANPLRSEEE 74

Query: 807  TDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSLACHFHAKYKSS 866
             DIV LKNYM+AQY+GEI +GTPPQKFTVIFDTGSSNLWVPSAKC FS+AC+ H++YK+ 
Sbjct: 75   GDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIACYLHSRYKAG 134

Query: 867  HSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATREPSLTFLVAKFDG 926
             SS+YKKNG  A+I+YGTG+++G+FS D+V VGDLVVK+Q+FIEAT+EP +TFLVAKFDG
Sbjct: 135  ASSTYKKNGKPAAIQYGTGSIAGYFSEDSVTVGDLVVKDQEFIEATKEPGITFLVAKFDG 194

Query: 927  LLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEE-EGGEIVFGGVDPKHYKG 986
            +LGLGF+EISVG AVPVWY M++Q LV +PVFSFWLNR+V+E EGGEI+FGG+DPKHY G
Sbjct: 195  ILGLGFKEISVGKAVPVWYKMIEQGLVSDPVFSFWLNRHVDEGEGGEIIFGGMDPKHYVG 254

Query: 987  EHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPTPIITMINHAIG 1046
            EHTYVPVTQKGYWQFDMGDVL+ G+ TG+C GGC+AIADSGTSLLAGPT IIT IN  IG
Sbjct: 255  EHTYVPVTQKGYWQFDMGDVLVGGKSTGFCAGGCAAIADSGTSLLAGPTAIITEINEKIG 314

Query: 1047 AKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVSMGIASVVDEKA 1106
            A GV+SQECK +V+QYGQ I+DLL +E  PKKICSQ+ LCTFDG RGVS GI SVVD++ 
Sbjct: 315  AAGVVSQECKTIVSQYGQQILDLLLAETQPKKICSQVGLCTFDGTRGVSAGIRSVVDDEP 374

Query: 1107 GKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSPMGQSAVDCGSL 1166
             KS+    D MC ACEM VVWMQNQL QN+T++ I++Y+N+LC+ +PSPMG+SAVDCGSL
Sbjct: 375  VKSNGLRADPMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLCNRLPSPMGESAVDCGSL 434

Query: 1167 SSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGPLWILGDVFMGR 1226
             SMP + FTIG K F L P+EY+LKVGEG AAQCISGFTA+D+PPPRGPLWILGDVFMG 
Sbjct: 435  GSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGP 494

Query: 1227 YHTVFDFGKLRVGFAEAA 1244
            YHTVFD+GKLR+GFA+AA
Sbjct: 495  YHTVFDYGKLRIGFAKAA 508

BLAST of CmaCh18G002710 vs. ExPASy TrEMBL
Match: A0A6J1JVK1 (ankyrin repeat-containing protein ITN1-like OS=Cucurbita maxima OX=3661 GN=LOC111490098 PE=4 SV=1)

HSP 1 Score: 1314.3 bits (3400), Expect = 0.0e+00
Identity = 665/665 (100.00%), Postives = 665/665 (100.00%), Query Frame = 0

Query: 1   MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDEDEDFEVGEDE 60
           MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDEDEDFEVGEDE
Sbjct: 1   MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDEDEDFEVGEDE 60

Query: 61  SPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQN 120
           SPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQN
Sbjct: 61  SPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQN 120

Query: 121 GDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKYG 180
           GDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKYG
Sbjct: 121 GDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKYG 180

Query: 181 NTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPVY 240
           NTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPVY
Sbjct: 181 NTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPVY 240

Query: 241 GSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKNK 300
           GSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKNK
Sbjct: 241 GSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKNK 300

Query: 301 PTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQFI 360
           PTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQFI
Sbjct: 301 PTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQFI 360

Query: 361 MFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEMG 420
           MFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEMG
Sbjct: 361 MFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEMG 420

Query: 421 EFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMRR 480
           EFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMRR
Sbjct: 421 EFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMRR 480

Query: 481 KKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNHD 540
           KKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNHD
Sbjct: 481 KKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNHD 540

Query: 541 INTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCGL 600
           INTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCGL
Sbjct: 541 INTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCGL 600

Query: 601 GTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAALQ 660
           GTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAALQ
Sbjct: 601 GTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAALQ 660

Query: 661 ASYYL 666
           ASYYL
Sbjct: 661 ASYYL 665

BLAST of CmaCh18G002710 vs. ExPASy TrEMBL
Match: A0A6J1GRW2 (ankyrin repeat-containing protein ITN1-like OS=Cucurbita moschata OX=3662 GN=LOC111456953 PE=4 SV=1)

HSP 1 Score: 1279.6 bits (3310), Expect = 0.0e+00
Identity = 649/666 (97.45%), Postives = 659/666 (98.95%), Query Frame = 0

Query: 1   MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDE-DEDFEVGED 60
           M+PFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDS AV+D N VNNDE D+DFEVGED
Sbjct: 1   MRPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSPAVEDRNAVNNDEDDDDFEVGED 60

Query: 61  ESPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ 120
           ESPARGDEENPLPLEIYGKPLDP+TKD+SWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ
Sbjct: 61  ESPARGDEENPLPLEIYGKPLDPRTKDVSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ 120

Query: 121 NGDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKY 180
           NGDWKTA+YMN L+PGVLTMVISDRCETVLHIATRAKKA FVKELVNFLDRHDLGLKNKY
Sbjct: 121 NGDWKTAEYMNNLHPGVLTMVISDRCETVLHIATRAKKASFVKELVNFLDRHDLGLKNKY 180

Query: 181 GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV 240
           GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV
Sbjct: 181 GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV 240

Query: 241 YGSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN 300
           YGSAIQEQMELL+GAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN
Sbjct: 241 YGSAIQEQMELLVGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN 300

Query: 301 KPTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQF 360
           KPT+ QSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMW+VVLDEIPEDEILQF
Sbjct: 301 KPTRLQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWTVVLDEIPEDEILQF 360

Query: 361 IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM 420
           IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM
Sbjct: 361 IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM 420

Query: 421 GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR 480
           GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR
Sbjct: 421 GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR 480

Query: 481 RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH 540
           RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH
Sbjct: 481 RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH 540

Query: 541 DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG 600
           DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG
Sbjct: 541 DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG 600

Query: 601 LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL 660
           LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL
Sbjct: 601 LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL 660

Query: 661 QASYYL 666
           QASYYL
Sbjct: 661 QASYYL 666

BLAST of CmaCh18G002710 vs. ExPASy TrEMBL
Match: A0A6J1K288 (aspartic proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111490008 PE=3 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 4.0e-296
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 735  MALPHFKAAFLCLFLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATF 794
            MALPHFKAAFLCLFLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATF
Sbjct: 1    MALPHFKAAFLCLFLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATF 60

Query: 795  RKSNSNLGQSSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFS 854
            RKSNSNLGQSSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFS
Sbjct: 61   RKSNSNLGQSSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFS 120

Query: 855  LACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATRE 914
            LACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATRE
Sbjct: 121  LACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATRE 180

Query: 915  PSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEEEGGEIV 974
            PSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEEEGGEIV
Sbjct: 181  PSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEEEGGEIV 240

Query: 975  FGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPT 1034
            FGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPT
Sbjct: 241  FGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPT 300

Query: 1035 PIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVS 1094
            PIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVS
Sbjct: 301  PIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVS 360

Query: 1095 MGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSP 1154
            MGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSP
Sbjct: 361  MGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSP 420

Query: 1155 MGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGP 1214
            MGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGP
Sbjct: 421  MGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGP 480

Query: 1215 LWILGDVFMGRYHTVFDFGKLRVGFAEAA 1244
            LWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 481  LWILGDVFMGRYHTVFDFGKLRVGFAEAA 509

BLAST of CmaCh18G002710 vs. ExPASy TrEMBL
Match: A0A6J1GRX1 (aspartic proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111456960 PE=3 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 3.3e-290
Identity = 504/512 (98.44%), Postives = 505/512 (98.63%), Query Frame = 0

Query: 735  MALPHFKAAFLCLFLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATF 794
            MALPHFKAAFLCL LLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATF
Sbjct: 1    MALPHFKAAFLCLLLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATF 60

Query: 795  RK--SNSNLGQSSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCL 854
            RK   NSNLG SSDTDIVVLKNY+DAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCL
Sbjct: 61   RKYNPNSNLGVSSDTDIVVLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCL 120

Query: 855  FSLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEAT 914
            FSLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEAT
Sbjct: 121  FSLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEAT 180

Query: 915  REPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNV-EEEGG 974
            REPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNV EEEGG
Sbjct: 181  REPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEEEEGG 240

Query: 975  EIVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLA 1034
            EIVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLA
Sbjct: 241  EIVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLA 300

Query: 1035 GPTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGAR 1094
            GPTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGAR
Sbjct: 301  GPTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGAR 360

Query: 1095 GVSMGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHM 1154
            GVSMGIASVVDE AGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHM
Sbjct: 361  GVSMGIASVVDENAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHM 420

Query: 1155 PSPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPP 1214
            PSPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPP
Sbjct: 421  PSPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPP 480

Query: 1215 RGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 1244
            RGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 481  RGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 512

BLAST of CmaCh18G002710 vs. ExPASy TrEMBL
Match: A0A6J1C7F7 (ankyrin repeat-containing protein NPR4-like OS=Momordica charantia OX=3673 GN=LOC111008689 PE=4 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 9.9e-279
Identity = 512/673 (76.08%), Postives = 565/673 (83.95%), Query Frame = 0

Query: 1   MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVT---GVDSAAVKDHNGVNNDEDEDFEVG 60
           M P TR+P +SS +      +D +AVRWSE VT     D+   +DH G+++ E  D    
Sbjct: 1   MMPLTRKPLSSSQS------NDPRAVRWSETVTDGDADDAGDGEDHRGIDDLEAAD---- 60

Query: 61  EDESPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQA 120
              SPA+GDE     + I  +       DL W  SK  SFRFS SRR+DNRPLRV+LYQA
Sbjct: 61  ---SPAQGDENQQQLVMISTRTSQSPKIDLRWAKSKIPSFRFSWSRRIDNRPLRVRLYQA 120

Query: 121 AQNGDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKN 180
           A  GDWKTA+ M I++PG LTMVISDRCET LHIATR KKA FV++LV  LD HDL LKN
Sbjct: 121 ALKGDWKTAKLMEIMHPGALTMVISDRCETALHIATRVKKAVFVEKLVERLDGHDLALKN 180

Query: 181 KYGNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKT 240
           KYGNTALCIAAASGAVDIAKLMV K++ALPLIRGSGN+TP+LIAARYKH+HMVSYLLSKT
Sbjct: 181 KYGNTALCIAAASGAVDIAKLMVRKYKALPLIRGSGNATPLLIAARYKHQHMVSYLLSKT 240

Query: 241 PVYGSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGK 300
           PVYG +IQEQMELL+GAISADYYDIALLIL+WN  L LERDFN+DTPLHIMARRSNAIG 
Sbjct: 241 PVYGLSIQEQMELLVGAISADYYDIALLILEWNKSLALERDFNNDTPLHIMARRSNAIGT 300

Query: 301 KNKPTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEIL 360
           KNKPT WQ+YI               K ++K K+MQIQAHQMVELMW VVLDEIPEDE+ 
Sbjct: 301 KNKPTIWQAYI-----------NSWFKHIYKKKMMQIQAHQMVELMWRVVLDEIPEDEMF 360

Query: 361 QFIMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIY 420
            FI  P+S+LHDAARVGNVEFLR++INSYPDLAWKVD  RKSIFHVAVENRQESVFSLIY
Sbjct: 361 DFIKNPSSMLHDAARVGNVEFLRVLINSYPDLAWKVDGKRKSIFHVAVENRQESVFSLIY 420

Query: 421 EMGEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELT 480
           EMGEFLDYLP+YFDEEN+SLLELAAK+AD +HLNRVSGAAFQMH+EL+WFKEVEKIVELT
Sbjct: 421 EMGEFLDYLPYYFDEENMSLLELAAKRADPSHLNRVSGAAFQMHRELIWFKEVEKIVELT 480

Query: 481 MRRKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNN- 540
           MRRKKGKR+PRELFT +H+NLVE+GEKWMKKTANSCMLVATLIATVVFAA+FTVPGGNN 
Sbjct: 481 MRRKKGKRSPRELFTLQHKNLVEDGEKWMKKTANSCMLVATLIATVVFAAVFTVPGGNNN 540

Query: 541 ----NNHDINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWL 600
               NN+D NTG+P+FL HKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWL
Sbjct: 541 NNIGNNNDNNTGAPIFLHHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWL 600

Query: 601 PLKLVCGLGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLW 660
           PLKLV GLGTLF SVLSMVLAFSATFFLFYGKDT WVPLLVAGMAIVPVYCFGVLQFRLW
Sbjct: 601 PLKLVFGLGTLFFSVLSMVLAFSATFFLFYGKDTAWVPLLVAGMAIVPVYCFGVLQFRLW 649

Query: 661 ADALAALQASYYL 666
           ADALAALQA+Y L
Sbjct: 661 ADALAALQATYLL 649

BLAST of CmaCh18G002710 vs. NCBI nr
Match: XP_022994352.1 (ankyrin repeat-containing protein ITN1-like [Cucurbita maxima])

HSP 1 Score: 1314.3 bits (3400), Expect = 0.0e+00
Identity = 665/665 (100.00%), Postives = 665/665 (100.00%), Query Frame = 0

Query: 1   MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDEDEDFEVGEDE 60
           MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDEDEDFEVGEDE
Sbjct: 1   MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDEDEDFEVGEDE 60

Query: 61  SPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQN 120
           SPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQN
Sbjct: 61  SPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQN 120

Query: 121 GDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKYG 180
           GDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKYG
Sbjct: 121 GDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKYG 180

Query: 181 NTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPVY 240
           NTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPVY
Sbjct: 181 NTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPVY 240

Query: 241 GSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKNK 300
           GSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKNK
Sbjct: 241 GSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKNK 300

Query: 301 PTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQFI 360
           PTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQFI
Sbjct: 301 PTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQFI 360

Query: 361 MFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEMG 420
           MFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEMG
Sbjct: 361 MFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEMG 420

Query: 421 EFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMRR 480
           EFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMRR
Sbjct: 421 EFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMRR 480

Query: 481 KKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNHD 540
           KKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNHD
Sbjct: 481 KKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNHD 540

Query: 541 INTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCGL 600
           INTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCGL
Sbjct: 541 INTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCGL 600

Query: 601 GTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAALQ 660
           GTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAALQ
Sbjct: 601 GTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAALQ 660

Query: 661 ASYYL 666
           ASYYL
Sbjct: 661 ASYYL 665

BLAST of CmaCh18G002710 vs. NCBI nr
Match: KAG6573177.1 (Ankyrin repeat-containing protein ITN1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 653/666 (98.05%), Postives = 659/666 (98.95%), Query Frame = 0

Query: 1   MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDE-DEDFEVGED 60
           M+PFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDS AV+D N VNNDE D+DFEVGED
Sbjct: 1   MRPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSPAVEDRNAVNNDEDDDDFEVGED 60

Query: 61  ESPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ 120
           ESPARGDEENPLPLEIYGKPLDPKTKD+SW MSKFHSFRFSKSRRMDNRPLRVQLYQAAQ
Sbjct: 61  ESPARGDEENPLPLEIYGKPLDPKTKDVSWRMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ 120

Query: 121 NGDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKY 180
           NGDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKA FVKELVNFLDRHDLGLKNKY
Sbjct: 121 NGDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKASFVKELVNFLDRHDLGLKNKY 180

Query: 181 GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV 240
           GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV
Sbjct: 181 GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV 240

Query: 241 YGSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN 300
           YGSAIQEQMELL+GAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN
Sbjct: 241 YGSAIQEQMELLVGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN 300

Query: 301 KPTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQF 360
           KPT+ QSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQF
Sbjct: 301 KPTRLQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQF 360

Query: 361 IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM 420
           IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM
Sbjct: 361 IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM 420

Query: 421 GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR 480
           GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR
Sbjct: 421 GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR 480

Query: 481 RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH 540
           RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH
Sbjct: 481 RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH 540

Query: 541 DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG 600
           DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG
Sbjct: 541 DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG 600

Query: 601 LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL 660
           LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL
Sbjct: 601 LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL 660

Query: 661 QASYYL 666
           QASYYL
Sbjct: 661 QASYYL 666

BLAST of CmaCh18G002710 vs. NCBI nr
Match: XP_022954801.1 (ankyrin repeat-containing protein ITN1-like [Cucurbita moschata])

HSP 1 Score: 1279.6 bits (3310), Expect = 0.0e+00
Identity = 649/666 (97.45%), Postives = 659/666 (98.95%), Query Frame = 0

Query: 1   MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDE-DEDFEVGED 60
           M+PFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDS AV+D N VNNDE D+DFEVGED
Sbjct: 1   MRPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSPAVEDRNAVNNDEDDDDFEVGED 60

Query: 61  ESPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ 120
           ESPARGDEENPLPLEIYGKPLDP+TKD+SWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ
Sbjct: 61  ESPARGDEENPLPLEIYGKPLDPRTKDVSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ 120

Query: 121 NGDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKY 180
           NGDWKTA+YMN L+PGVLTMVISDRCETVLHIATRAKKA FVKELVNFLDRHDLGLKNKY
Sbjct: 121 NGDWKTAEYMNNLHPGVLTMVISDRCETVLHIATRAKKASFVKELVNFLDRHDLGLKNKY 180

Query: 181 GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV 240
           GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV
Sbjct: 181 GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV 240

Query: 241 YGSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN 300
           YGSAIQEQMELL+GAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN
Sbjct: 241 YGSAIQEQMELLVGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN 300

Query: 301 KPTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQF 360
           KPT+ QSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMW+VVLDEIPEDEILQF
Sbjct: 301 KPTRLQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWTVVLDEIPEDEILQF 360

Query: 361 IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM 420
           IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM
Sbjct: 361 IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM 420

Query: 421 GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR 480
           GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR
Sbjct: 421 GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR 480

Query: 481 RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH 540
           RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH
Sbjct: 481 RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH 540

Query: 541 DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG 600
           DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG
Sbjct: 541 DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG 600

Query: 601 LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL 660
           LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL
Sbjct: 601 LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL 660

Query: 661 QASYYL 666
           QASYYL
Sbjct: 661 QASYYL 666

BLAST of CmaCh18G002710 vs. NCBI nr
Match: XP_023542599.1 (ankyrin repeat-containing protein ITN1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1277.3 bits (3304), Expect = 0.0e+00
Identity = 649/666 (97.45%), Postives = 658/666 (98.80%), Query Frame = 0

Query: 1   MKPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSAAVKDHNGVNNDE-DEDFEVGED 60
           M PFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDS AV+D N VNNDE D+DFEVGED
Sbjct: 1   MIPFTRRPSTSSSTKSTPGRHDGKAVRWSEPVTGVDSPAVEDRNAVNNDEDDDDFEVGED 60

Query: 61  ESPARGDEENPLPLEIYGKPLDPKTKDLSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ 120
           ESPARGDEENPLPLEIYGKPLDPKTKD+SWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ
Sbjct: 61  ESPARGDEENPLPLEIYGKPLDPKTKDVSWPMSKFHSFRFSKSRRMDNRPLRVQLYQAAQ 120

Query: 121 NGDWKTAQYMNILYPGVLTMVISDRCETVLHIATRAKKAFFVKELVNFLDRHDLGLKNKY 180
           NGDWKTA+YMN L+PGVLTMVISDRCETVLHIATRAKKA FVKELVNFLDRHDLGLKNKY
Sbjct: 121 NGDWKTAEYMNNLHPGVLTMVISDRCETVLHIATRAKKASFVKELVNFLDRHDLGLKNKY 180

Query: 181 GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV 240
           GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV
Sbjct: 181 GNTALCIAAASGAVDIAKLMVSKFEALPLIRGSGNSTPVLIAARYKHKHMVSYLLSKTPV 240

Query: 241 YGSAIQEQMELLIGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN 300
           YGSAIQEQMELL+GAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN
Sbjct: 241 YGSAIQEQMELLVGAISADYYDIALLILKWNPLLVLERDFNDDTPLHIMARRSNAIGKKN 300

Query: 301 KPTKWQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQF 360
           KPT+ QSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQF
Sbjct: 301 KPTRLQSYIIDCEEQTSNLLVKGIKRMHKHKLMQIQAHQMVELMWSVVLDEIPEDEILQF 360

Query: 361 IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM 420
           IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM
Sbjct: 361 IMFPTSILHDAARVGNVEFLRLIINSYPDLAWKVDSDRKSIFHVAVENRQESVFSLIYEM 420

Query: 421 GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR 480
           GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR
Sbjct: 421 GEFLDYLPFYFDEENISLLELAAKKADLNHLNRVSGAAFQMHKELLWFKEVEKIVELTMR 480

Query: 481 RKKGKRNPRELFTKEHRNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH 540
           RKKGKRNPRELFTKEH+NLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH
Sbjct: 481 RKKGKRNPRELFTKEHQNLVEEGEKWMKKTANSCMLVATLIATVVFAAIFTVPGGNNNNH 540

Query: 541 DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG 600
           DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG
Sbjct: 541 DINTGSPLFLRHKWFTVFVISDATALISSSTSILLFLSILTSRCAEEDFLIWLPLKLVCG 600

Query: 601 LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADALAAL 660
           LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADA+AAL
Sbjct: 601 LGTLFLSVLSMVLAFSATFFLFYGKDTDWVPLLVAGMAIVPVYCFGVLQFRLWADAVAAL 660

Query: 661 QASYYL 666
           QASYYL
Sbjct: 661 QASYYL 666

BLAST of CmaCh18G002710 vs. NCBI nr
Match: KAG6573178.1 (hypothetical protein SDJN03_27065, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 556/573 (97.03%), Postives = 560/573 (97.73%), Query Frame = 0

Query: 674  PGGNTTLTPHNGRLRSNHFLRLLNWVCLICFSFVYKEVGREICVPTPQFSPPFEILRCWL 733
            PG    +TPHNGRLRSNH LRLLNWVCLICFSFVYKEVGREICVPTPQFSP FEILRCWL
Sbjct: 20   PGRKHNVTPHNGRLRSNHLLRLLNWVCLICFSFVYKEVGREICVPTPQFSPSFEILRCWL 79

Query: 734  PMALPHFKAAFLCLFLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKAT 793
            PMALPHFKAAFLCL LLVSFNI SSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKAT
Sbjct: 80   PMALPHFKAAFLCLLLLVSFNIGSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKAT 139

Query: 794  FRK--SNSNLGQSSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKC 853
            FRK   NSNLG SSDTDIVVLKNY+DAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKC
Sbjct: 140  FRKYNPNSNLGVSSDTDIVVLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKC 199

Query: 854  LFSLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEA 913
            LFSLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEA
Sbjct: 200  LFSLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEA 259

Query: 914  TREPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNV-EEEG 973
            TREPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNV EEEG
Sbjct: 260  TREPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEEEEG 319

Query: 974  GEIVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLL 1033
            GEIVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLL
Sbjct: 320  GEIVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLL 379

Query: 1034 AGPTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGA 1093
            AGPTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEA+PKKICSQIKLCTF+GA
Sbjct: 380  AGPTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEANPKKICSQIKLCTFNGA 439

Query: 1094 RGVSMGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDH 1153
            RGVSMGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDH
Sbjct: 440  RGVSMGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDH 499

Query: 1154 MPSPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPP 1213
            MPSPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPP
Sbjct: 500  MPSPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPP 559

Query: 1214 PRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 1244
            PRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA
Sbjct: 560  PRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 592

BLAST of CmaCh18G002710 vs. TAIR 10
Match: AT1G11910.1 (aspartic proteinase A1 )

HSP 1 Score: 763.5 bits (1970), Expect = 2.7e-220
Identity = 374/501 (74.65%), Postives = 424/501 (84.63%), Query Frame = 0

Query: 749  LLVSFNIVSSA-----SGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATFRKSNSNLGQ 808
            L+VSF +  SA      G  RVGLKK+KLDSK++LAAR++SK  + L+A        LG 
Sbjct: 12   LIVSFLLCFSAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRA------YRLGD 71

Query: 809  SSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSLACHFHAKY 868
            S D D+VVLKNY+DAQYYGEIAIGTPPQKFTV+FDTGSSNLWVPS+KC FSLAC  H KY
Sbjct: 72   SGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSLACLLHPKY 131

Query: 869  KSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATREPSLTFLVAK 928
            KSS SS+Y+KNG +A+I YGTGA++GFFSND V VGDLVVK+Q+FIEAT+EP +TF+VAK
Sbjct: 132  KSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEPGITFVVAK 191

Query: 929  FDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNV-EEEGGEIVFGGVDPKH 988
            FDG+LGLGFQEISVG A PVWYNM+ Q L+KEPVFSFWLNRN  EEEGGE+VFGGVDP H
Sbjct: 192  FDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELVFGGVDPNH 251

Query: 989  YKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPTPIITMINH 1048
            +KG+HTYVPVTQKGYWQFDMGDVLI G PTG+C  GCSAIADSGTSLLAGPT IITMINH
Sbjct: 252  FKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTTIITMINH 311

Query: 1049 AIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVSMGIASVVD 1108
            AIGA GV+SQ+CK VV QYGQTI+DLL SE  PKKICSQI LCTFDG RGVSMGI SVVD
Sbjct: 312  AIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVSMGIESVVD 371

Query: 1109 EKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSPMGQSAVDC 1168
            ++  K S+G+ DA C ACEM VVW+Q+QLRQN T+ERI+NY+NELC+ +PSPMG+SAVDC
Sbjct: 372  KENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMGESAVDC 431

Query: 1169 GSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGPLWILGDVF 1228
              LS+MP VS TIG KVFDL P+EYVLKVGEG  AQCISGF ALDV PPRGPLWILGDVF
Sbjct: 432  AQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLWILGDVF 491

Query: 1229 MGRYHTVFDFGKLRVGFAEAA 1244
            MG+YHTVFDFG  +VGFAEAA
Sbjct: 492  MGKYHTVFDFGNEQVGFAEAA 506

BLAST of CmaCh18G002710 vs. TAIR 10
Match: AT1G62290.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 755.4 bits (1949), Expect = 7.4e-218
Identity = 366/497 (73.64%), Postives = 416/497 (83.70%), Query Frame = 0

Query: 748  FLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATFRKSNSNL-GQSSD 807
            FLL          G  RVGLKK+KLD  ++LA R  SK  E L+++ R  N+NL G S D
Sbjct: 16   FLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRSSLRSYNNNLGGDSGD 75

Query: 808  TDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSLACHFHAKYKSS 867
             DIV LKNY+DAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPS KC FSL+C+FHAKYKSS
Sbjct: 76   ADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKCFFSLSCYFHAKYKSS 135

Query: 868  HSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATREPSLTFLVAKFDG 927
             SS+YKK+G  A+I YG+G++SGFFS D V VGDLVVK+Q+FIE T EP LTFLVAKFDG
Sbjct: 136  RSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIETTSEPGLTFLVAKFDG 195

Query: 928  LLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVE-EEGGEIVFGGVDPKHYKG 987
            LLGLGFQEI+VGNA PVWYNM+ Q L+K PVFSFWLNR+ + EEGGEIVFGGVDPKH++G
Sbjct: 196  LLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSEEGGEIVFGGVDPKHFRG 255

Query: 988  EHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPTPIITMINHAIG 1047
            EHT+VPVTQ+GYWQFDMG+VLI GE TGYCG GCSAIADSGTSLLAGPT ++ MIN AIG
Sbjct: 256  EHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGPTAVVAMINKAIG 315

Query: 1048 AKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVSMGIASVVDEKA 1107
            A GV+SQ+CK VV QYGQTI+DLL +E  PKKICSQI LC +DG  GVSMGI SVVD++ 
Sbjct: 316  ASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYDGTHGVSMGIESVVDKEN 375

Query: 1108 GKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSPMGQSAVDCGSL 1167
             +SS GLRDA CPACEM VVW+Q+QLRQN T+ERI+NYINE+C+ MPSP G+SAVDC  L
Sbjct: 376  TRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAVDCSQL 435

Query: 1168 SSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGPLWILGDVFMGR 1227
            S MP VSFTIG KVFDL P+EYVLK+GEG  AQCISGFTALD+PPPRGPLWILGDVFMG+
Sbjct: 436  SKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGK 495

Query: 1228 YHTVFDFGKLRVGFAEA 1243
            YHTVFDFG  +VGFAEA
Sbjct: 496  YHTVFDFGNEQVGFAEA 512

BLAST of CmaCh18G002710 vs. TAIR 10
Match: AT1G62290.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 755.4 bits (1949), Expect = 7.4e-218
Identity = 366/497 (73.64%), Postives = 416/497 (83.70%), Query Frame = 0

Query: 748  FLLVSFNIVSSASGLLRVGLKKIKLDSKSQLAARLQSKDPEILKATFRKSNSNL-GQSSD 807
            FLL          G  RVGLKK+KLD  ++LA R  SK  E L+++ R  N+NL G S D
Sbjct: 16   FLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRSSLRSYNNNLGGDSGD 75

Query: 808  TDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLFSLACHFHAKYKSS 867
             DIV LKNY+DAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPS KC FSL+C+FHAKYKSS
Sbjct: 76   ADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKCFFSLSCYFHAKYKSS 135

Query: 868  HSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATREPSLTFLVAKFDG 927
             SS+YKK+G  A+I YG+G++SGFFS D V VGDLVVK+Q+FIE T EP LTFLVAKFDG
Sbjct: 136  RSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIETTSEPGLTFLVAKFDG 195

Query: 928  LLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVE-EEGGEIVFGGVDPKHYKG 987
            LLGLGFQEI+VGNA PVWYNM+ Q L+K PVFSFWLNR+ + EEGGEIVFGGVDPKH++G
Sbjct: 196  LLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSEEGGEIVFGGVDPKHFRG 255

Query: 988  EHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAGPTPIITMINHAIG 1047
            EHT+VPVTQ+GYWQFDMG+VLI GE TGYCG GCSAIADSGTSLLAGPT ++ MIN AIG
Sbjct: 256  EHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGPTAVVAMINKAIG 315

Query: 1048 AKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARGVSMGIASVVDEKA 1107
            A GV+SQ+CK VV QYGQTI+DLL +E  PKKICSQI LC +DG  GVSMGI SVVD++ 
Sbjct: 316  ASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYDGTHGVSMGIESVVDKEN 375

Query: 1108 GKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMPSPMGQSAVDCGSL 1167
             +SS GLRDA CPACEM VVW+Q+QLRQN T+ERI+NYINE+C+ MPSP G+SAVDC  L
Sbjct: 376  TRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAVDCSQL 435

Query: 1168 SSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPRGPLWILGDVFMGR 1227
            S MP VSFTIG KVFDL P+EYVLK+GEG  AQCISGFTALD+PPPRGPLWILGDVFMG+
Sbjct: 436  SKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGDVFMGK 495

Query: 1228 YHTVFDFGKLRVGFAEA 1243
            YHTVFDFG  +VGFAEA
Sbjct: 496  YHTVFDFGNEQVGFAEA 512

BLAST of CmaCh18G002710 vs. TAIR 10
Match: AT4G04460.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 692.6 bits (1786), Expect = 5.9e-199
Identity = 336/511 (65.75%), Postives = 417/511 (81.60%), Query Frame = 0

Query: 743  AFLCLFLLVSFNIVSSAS------GLLRVGLKKIKLDSKSQLAARLQSKDPE---ILKAT 802
            +FL +FLL    ++S+AS      G +R+GLKK KLD  ++LA++L  K+       K  
Sbjct: 7    SFLLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKNRGSHWSPKHY 66

Query: 803  FRKSNSNLGQSSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLF 862
            FR ++ N       D+V LKNY+DAQYYG+I IGTPPQKFTVIFDTGSSNLW+PS KC  
Sbjct: 67   FRLNDEN------ADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYL 126

Query: 863  SLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATR 922
            S+AC+FH+KYK+S SSSY+KNG  ASIRYGTGA+SG+FSND+V+VGD+VVK Q+FIEAT 
Sbjct: 127  SVACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATS 186

Query: 923  EPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEE-EGGE 982
            EP +TFL+AKFDG+LGLGF+EISVGN+ PVWYNMV++ LVKEP+FSFWLNRN ++ EGGE
Sbjct: 187  EPGITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGE 246

Query: 983  IVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAG 1042
            IVFGGVDPKH+KGEHT+VPVT KGYWQFDMGD+ I G+PTGYC  GCSAIADSGTSLL G
Sbjct: 247  IVFGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTG 306

Query: 1043 PTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARG 1102
            P+ +ITMINHAIGA+G++S+ECKAVV QYG+T+++ L ++ DPKK+CSQI +C +DG + 
Sbjct: 307  PSTVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLAQEDPKKVCSQIGVCAYDGTQS 366

Query: 1103 VSMGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMP 1162
            VSMGI SVVD+    +S  L  AMC ACEM  VWM+++L QNQT+ERI+ Y  ELCDH+P
Sbjct: 367  VSMGIQSVVDD---GTSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIP 426

Query: 1163 SPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPR 1222
            +   QSAVDCG +SSMPIV+F+IG + FDLTPQ+Y+ K+GEG  +QC SGFTA+D+ PPR
Sbjct: 427  TQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPR 486

Query: 1223 GPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 1244
            GPLWILGD+FMG YHTVFD+GK RVGFA+AA
Sbjct: 487  GPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 508

BLAST of CmaCh18G002710 vs. TAIR 10
Match: AT4G04460.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 679.9 bits (1753), Expect = 3.9e-195
Identity = 333/511 (65.17%), Postives = 414/511 (81.02%), Query Frame = 0

Query: 743  AFLCLFLLVSFNIVSSAS------GLLRVGLKKIKLDSKSQLAARLQSKDPE---ILKAT 802
            +FL +FLL    ++S+AS      G +R+GLKK KLD  ++LA++L  K+       K  
Sbjct: 7    SFLLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKNRGSHWSPKHY 66

Query: 803  FRKSNSNLGQSSDTDIVVLKNYMDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSAKCLF 862
            FR ++ N       D+V LKNY+DAQYYG+I IGTPPQKFTVIFDTGSSNLW+PS KC  
Sbjct: 67   FRLNDEN------ADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYL 126

Query: 863  SLACHFHAKYKSSHSSSYKKNGTSASIRYGTGAVSGFFSNDNVRVGDLVVKNQDFIEATR 922
            S+AC+FH+KYK+S SSSY+KNG  ASIRYGTGA+SG+FSND+V+VGD+VVK Q+FIEAT 
Sbjct: 127  SVACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATS 186

Query: 923  EPSLTFLVAKFDGLLGLGFQEISVGNAVPVWYNMVDQDLVKEPVFSFWLNRNVEE-EGGE 982
            EP +TFL+AKFDG+LGLGF+EISVGN+ PVWYNMV++ LVKEP+FSFWLNRN ++ EGGE
Sbjct: 187  EPGITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGE 246

Query: 983  IVFGGVDPKHYKGEHTYVPVTQKGYWQFDMGDVLIDGEPTGYCGGGCSAIADSGTSLLAG 1042
            IVFGGVDPKH+KGEHT+VPVT KGYWQFDMGD+ I G+PTGYC  GCSAIADSGTSLL G
Sbjct: 247  IVFGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTG 306

Query: 1043 PTPIITMINHAIGAKGVISQECKAVVAQYGQTIMDLLSSEADPKKICSQIKLCTFDGARG 1102
            P+ +ITMINHAIGA+G++S+ECKAVV QYG+T+++ L ++    K+CSQI +C +DG + 
Sbjct: 307  PSTVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLAQ----KVCSQIGVCAYDGTQS 366

Query: 1103 VSMGIASVVDEKAGKSSDGLRDAMCPACEMMVVWMQNQLRQNQTKERIMNYINELCDHMP 1162
            VSMGI SVVD+    +S  L  AMC ACEM  VWM+++L QNQT+ERI+ Y  ELCDH+P
Sbjct: 367  VSMGIQSVVDD---GTSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIP 426

Query: 1163 SPMGQSAVDCGSLSSMPIVSFTIGDKVFDLTPQEYVLKVGEGRAAQCISGFTALDVPPPR 1222
            +   QSAVDCG +SSMPIV+F+IG + FDLTPQ+Y+ K+GEG  +QC SGFTA+D+ PPR
Sbjct: 427  TQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPR 486

Query: 1223 GPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 1244
            GPLWILGD+FMG YHTVFD+GK RVGFA+AA
Sbjct: 487  GPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O040571.9e-25886.96Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
O653903.8e-21974.65Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1[more]
Q8VYL31.0e-21673.64Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1[more]
Q424565.4e-20569.40Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g... [more]
P422101.1e-20267.87Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JVK10.0e+00100.00ankyrin repeat-containing protein ITN1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GRW20.0e+0097.45ankyrin repeat-containing protein ITN1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1K2884.0e-296100.00aspartic proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111490008 PE=3 SV=1[more]
A0A6J1GRX13.3e-29098.44aspartic proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111456960 PE=3 SV=1[more]
A0A6J1C7F79.9e-27976.08ankyrin repeat-containing protein NPR4-like OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_022994352.10.0e+00100.00ankyrin repeat-containing protein ITN1-like [Cucurbita maxima][more]
KAG6573177.10.0e+0098.05Ankyrin repeat-containing protein ITN1, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022954801.10.0e+0097.45ankyrin repeat-containing protein ITN1-like [Cucurbita moschata][more]
XP_023542599.10.0e+0097.45ankyrin repeat-containing protein ITN1-like [Cucurbita pepo subsp. pepo][more]
KAG6573178.10.0e+0097.03hypothetical protein SDJN03_27065, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT1G11910.12.7e-22074.65aspartic proteinase A1 [more]
AT1G62290.17.4e-21873.64Saposin-like aspartyl protease family protein [more]
AT1G62290.27.4e-21873.64Saposin-like aspartyl protease family protein [more]
AT4G04460.15.9e-19965.75Saposin-like aspartyl protease family protein [more]
AT4G04460.23.9e-19565.17Saposin-like aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 826..846
score: 73.01
coord: 1216..1231
score: 58.88
coord: 971..984
score: 47.22
coord: 1021..1032
score: 58.07
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47966BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATEDcoord: 741..1243
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 396..425
e-value: 320.0
score: 8.7
coord: 144..173
e-value: 720.0
score: 6.1
coord: 213..242
e-value: 120.0
score: 11.6
coord: 362..391
e-value: 330.0
score: 8.6
coord: 179..208
e-value: 0.76
score: 18.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 978..1243
e-value: 1.1E-119
score: 400.0
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 763..977
e-value: 4.5E-79
score: 267.1
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 747..1242
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 354..460
e-value: 9.8E-6
score: 26.3
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 107..345
e-value: 1.3E-20
score: 75.9
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 114..294
NoneNo IPR availableGENE3D1.10.225.10coord: 1049..1152
e-value: 1.1E-119
score: 400.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availablePANTHERPTHR47966:SF31ASPARTIC PROTEINASE-LIKEcoord: 741..1243
NoneNo IPR availableSUPERFAMILY140860Pseudo ankyrin repeat-likecoord: 336..453
IPR026961PGG domainPFAMPF13962PGGcoord: 503..619
e-value: 4.0E-29
score: 100.8
IPR007856Saposin-like type B, region 1PFAMPF05184SapB_1coord: 1116..1152
e-value: 2.1E-12
score: 46.8
IPR033121Peptidase family A1 domainPFAMPF00026Aspcoord: 819..1242
e-value: 3.2E-130
score: 434.9
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 820..1240
score: 72.509521
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 114..202
e-value: 6.5E-8
score: 33.1
IPR008138Saposin B type, region 2PFAMPF03489SapB_2coord: 1052..1085
e-value: 8.1E-12
score: 45.2
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 835..846
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 1021..1032
IPR008139Saposin B type domainPROSITEPS50015SAP_Bcoord: 1049..1089
score: 12.8404
IPR008139Saposin B type domainPROSITEPS50015SAP_Bcoord: 1113..1154
score: 12.138399
IPR033869PhytepsinCDDcd06098phytepsincoord: 810..1241
e-value: 0.0
score: 639.034
IPR011001Saposin-likeSUPERFAMILY47862Saposincoord: 1050..1152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G002710.1CmaCh18G002710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0005515 protein binding