CmaCh17G013260 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh17G013260
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Ribonuclease T(2)
Location	Cma_Chr17: 8934801 .. 8939141 (-)
RNA-Seq Expression	CmaCh17G013260
Synteny	CmaCh17G013260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTCCGTTCATCTTTCTTCTTCAATTCACCGTCTCTGTTGCATCCACTTCCTTGAATGATTCTTCGGATGCGATAATTTCGCAGATCAAGGACGGACGAGAACAGAGAGAGTTGGATTACTTCAAATTGACGCTGCAATGGCCTGGCACTGTCTGCAAGGGCACCGACAGCTGTTGTTCCTCCAACGCATGCTGTAATAGGTGAGTGCTTCTTCTCTTTTTCCTACAACATTCCCCGTTTAGAATACTCGATTAATCGTTACTTTCGTTTGAGGTTCTACAGCTAAGTAACTTCTACAGCCTTTTCACATTCCCCGTTTGTGAATTAATTAATTAAGCTGTAGAAGTTACCACATTCCCCGTTTACAACATACGAAGAGGAGACCAATTAGGAGAGATCCTACACAACTTAAAATTATGCATCCAAATCTCTTCAGTCAATTCTATAAGATAAATTCTTGATATTTTATAATAACAAGATTCTATCATCTATCATTTGTAGTAAATCCCTATGGAAAGATTTACAGACGTGAAAAATATAAGTTCAATCAATTCTATGAGGGGGGGAATCATGGTAATGATCAAAGAGGAGAAACATTTTGCCATTATGAATCTTTGTTAGGCATAATAAGTGGACAGAAACCGAAGAACTCTTCTTCGTTAATCTAGGAGTGGATATTTGTAATCTAAGCTGAATTTTTTTGTTCATTGTTAGAGTAGCTATAGACTTTGTTAGATTTCATGTCCTTCTGCCATCCCTTATACTTTATAATGTATCTTTTTGTTCCATCTATTAATTTTGGAAGACTTCTTCGTAAGTTACTTTTAAGTTGTAGTAGCTTTCCAATGATCTTTCATGGATTTAAATTTGAGCGTCTTCTTGCACCATCATCTACATTCTGTGTTTATTGGGCAATTGGGTATTTTGACATCGAAAGAATTACCATGGAGATGACACCAAAAAGTAGATGAGAAAAAGTTATTAGACCATTCATAGAACAAAAATTTAAAGTTTACGGTTGTGAAAGTTTTGCCTATAGGTCATGTTCGGTATTTTCTTGAGCGTTCTTCTGAATTCTCTAGATTTTCTTTTTCATTGTTCATAAACTTATGCTCGTTTATCCCTTCTTTGGTAGATCCGGTGGTTTTGCTGACTTTACGATTCGTAAGTTGAAGCTACAATTGCATTTCTTCCCACAACTTATAAGTGCAAGCGGTGTTTATAAGCTCGATCACAGAGCATATCAATATCAATTCTCTAAGAATAAACTTGGAGTCGTTCATTTGTTTGAGAATTTTCTTTATATAAATATAAAATATTAAGAGTTCGTTATTGTATGACTACGAATTTATAATCTGTTGCTTGCGTGATAGATGGGTTATGGCCTGATTATAATGATGGAACATGGCCTTCATGTTGCAATGGAACAAGTTTTGATGAGAAGGAGGTGCGTTGTTATTAAAGTTAGGCTGTTGATATTCATGATATGATCCATAGTTGAAGAGCTAAAACAAAACTTTTTCAGCCTATTGGCTTTATTTTGATCATTGTGCCGTTTACTTTGTTCTTGTTCGGCACAAGGCATTTTTGTATCATCCTGCATAATTTTATCTTGCAGATTCATACCTTACTTGATCCCCTGAAGAAATACTGGCCATCTTTGAGTTGTTCATCTTCAACTTGCCATGGTGGGAAGGGATCATTTTGGGCTCATGAGGTGACCATCACTAATACAAGATATATTTGTGTAAATTTGCACCGATGGTACCCAAGTTTTAACTGATTCTTTTCTCCTCACTTTTTGCTCCTATGATGGCTGAATTGGCTACTGCATTGACTAGTGGGGTAATAATCCTCGGCTTCTCTCACTTGGATACTATAATCTATATTCTATAATGCATTGAGGCTTTTCTTCTTTTATTGAATTTTTTTGATTGGCAAAGGTTAAATTTTTAGATTTTTTTGATTAACTATAAGATTTTTAAAGTCGTTCACATCATGGCCATATATGATCTATGCATTGCATAATGATATCGTATACAAAAGTGTATTTTTCTATCATTTACCTTGGGCCAATGCTCTGCTCAACTTTGATGTTGGCCTTTCTTATCTACGTAGAGTTTGTTCTTGATTGGATGTGACAGCATGTCTTTTTGGTAATTTGCTACGTTTTAGGACACAGGAAATAGTTTGATATGTTCATATTATTGTTTTGATGCCAATGTTGGAATCAAAGCATGTTTAATGTTCTTCCCATGCTTTGGTTTTCTCATATTGTATTTCTATGGCATGCAGAGAAGCATGGAACCTGTTCTTATCCAGTAATTCGTGATGAATACAGTTACTTTTTAACTACTCTCAACGTCTTTTTCAAATATAATGTCACTGTAAGTTTTGGAATGATGCTACCCTTTCTACATTAGCTCTAATATCAATAGGTCAGATCATGCTGCTTGCAGTTTAGTTCCCCATTTTTGCTATTGTCGATTTTTCCCTTATTTGATTAAGTTTACTGATGACTTTCCTTTGAAAACTTTCCTTTGAAAATCGAGTATAACTCAGTGAATAAGACACATCTTACTACTCGATTTGACCAAATCCTGCCATTATTGAACCCAAAAGAGAGGTTGTTTGATAACCATTTAGTTTTTTGTTTTTGGTTTTTGAAAATTTGCTTACAAACGCCGCTTGCACTTATAAGTTTCTTTAATTTGCTATTCATATAATGTATTTAAAACCTTTATCATTTCTTATTTTGCATTGGAATGAGTTTGGTTACTAATGAACATGTTATGTCCCAGAAAGTTCTGAATGATGCTGGATATCTACCTTCAAATACCGAGAAATATCCTCTTGGAGGAATCGTTTCAGCAGTTGAGAATGCTTTTCATGTCACCCCTTCTTTAGCATGTTCAGAGGGTGCACTGCAGGAACTTCGTTTATGTTTCTACAAGGATCTCAAGGTTGGTCCCTTAACTTAAAAAATGTATAATAGGTTCTTAAATTTTTAGAAGTCATGAATCTAACACAAAATTGAAAGTTTAATATTCTATTAGACACTGAGATTCAAAATTCAAAGTTTAAAAATAAATAGGATGCAAATCTTAAAGTTCGAGAACGAAACTTTTAGGAAAAAAAATATTGGATATGTATGGATACATTTTTTTCACAGGTGTGATCTTAGTGATGTTTATGGGACATCTTTTGGGTTCCTCAAATTATTATGTTTGCTTCATTGCAGCCTCGTGACTGTGTTCAGACAGACTCACAGAATGGGATCATTTCTTCAAATTTTTCGTGTCCCCAATATGTGAGCTTACCAGCTATTGATCCTAGTGGCAATATTTTCAAGTTCGTTGATTCAAGTAGGCAATATCCAAGTGCTTAATGTTCAGACTATTGAACATTAAAGATTATACATATATATATGAATATGTAGTGTATTATGTTTCTAACACACATGGTGGATTAGAACTTTGTTAACGATGCATTTTTAATCTTCATCTTGATTACTGGTATCAAATTGAAAAATGTCCTACTGTTGTAGTTTCTGCACTGAGATTTAATTAGCCAAGAAATTACTGGGATTATCCAAGGGATGTTGGAACTTGGAAGATTAGTAGGTCAATTTCTACTAATTCTAAGAAATGATTCTAAAACACCCTTGAATCGTAAGAATTACCAAGAGGTGTGGATAAATGATCCTCTTTAAGTATTTTCTAATCAAATATTGAAGTGAAATTCAGAGTCAGTCTCAAAAGACGGTGTAAATAAACTTCAATATTTTACCCCAGCTTTCTCGGAAAATCTTCGTAGGAAAAGGATCTCCGAGAAGGGAATTTGCTAATTTTAGGGTTTCCAAAATCCTCTTTTCTTTTCCTAAATTGAGACTATCTAGAAATTTTGAACATACATGGTGGGTCATACTTGTAGAAATTTCTTCAAAATTGAGACTCTATTGTTGTCCCTGATAAGAAGACAAGTTTTCTAGTAGGATTCAAGTTTGAATTTAATGTCAAATTGCGAGGTTAAAAAGAAGCCAATGAAACTAACAATAAATCAAGAATCATGAAGCAAACAAAAGGTTAAAACTGCCAATATTTTTAATTTCCACGACCAAGATGATTCTATCCTTAAAGTCGATGATTCAATCCTTAAAGTCTCGTGTCAATAAGAAATACAATAGTTTAGTTTGATATAACATGAAGCTCTCCTTTTGTACAAAGTACCCGGATTTTATTTAGGAGAAAGCAAAAAAAGGAACGGAGTTGCACCTTTTACATGGGGTTTTGTCCTGCAATTGTTGGTACAAAATTTCACCTACAAGATATGTTATGA

mRNA sequence

ATGATTCCGTTCATCTTTCTTCTTCAATTCACCGTCTCTGTTGCATCCACTTCCTTGAATGATTCTTCGGATGCGATAATTTCGCAGATCAAGGACGGACGAGAACAGAGAGAGTTGGATTACTTCAAATTGACGCTGCAATGGCCTGGCACTGTCTGCAAGGGCACCGACAGCTGTTGTTCCTCCAACGCATGCTGTAATAGATCCGGTGGTTTTGCTGACTTTACGATTCATGGGTTATGGCCTGATTATAATGATGGAACATGGCCTTCATGTTGCAATGGAACAAGTTTTGATGAGAAGGAGATTCATACCTTACTTGATCCCCTGAAGAAATACTGGCCATCTTTGAGTTGTTCATCTTCAACTTGCCATGAGAAGCATGGAACCTGTTCTTATCCAGTAATTCGTGATGAATACAGTTACTTTTTAACTACTCTCAACGTCTTTTTCAAATATAATGTCACTAAAGTTCTGAATGATGCTGGATATCTACCTTCAAATACCGAGAAATATCCTCTTGGAGGAATCGTTTCAGCAGTTGAGAATGCTTTTCATGTCACCCCTTCTTTAGCATGTTCAGAGGGTGCACTGCAGGAACTTCGTTTATGTTTCTACAAGGATCTCAAGCCTCGTGACTGTGTTCAGACAGACTCACAGAATGGGATCATTTCTTCAAATTTTTCGTGTCCCCAATATGTGAGCTTACCAGCTATTGATCCTAGTGGCAATATTTTCAACTCTCCTTTTGTACAAAGTACCCGGATTTTATTTAGGAGAAAGCAAAAAAAGGAACGGAGTTGCACCTTTTACATGGGGTTTTGTCCTGCAATTGTTGGTACAAAATTTCACCTACAAGATATGTTATGA

Coding sequence (CDS)

ATGATTCCGTTCATCTTTCTTCTTCAATTCACCGTCTCTGTTGCATCCACTTCCTTGAATGATTCTTCGGATGCGATAATTTCGCAGATCAAGGACGGACGAGAACAGAGAGAGTTGGATTACTTCAAATTGACGCTGCAATGGCCTGGCACTGTCTGCAAGGGCACCGACAGCTGTTGTTCCTCCAACGCATGCTGTAATAGATCCGGTGGTTTTGCTGACTTTACGATTCATGGGTTATGGCCTGATTATAATGATGGAACATGGCCTTCATGTTGCAATGGAACAAGTTTTGATGAGAAGGAGATTCATACCTTACTTGATCCCCTGAAGAAATACTGGCCATCTTTGAGTTGTTCATCTTCAACTTGCCATGAGAAGCATGGAACCTGTTCTTATCCAGTAATTCGTGATGAATACAGTTACTTTTTAACTACTCTCAACGTCTTTTTCAAATATAATGTCACTAAAGTTCTGAATGATGCTGGATATCTACCTTCAAATACCGAGAAATATCCTCTTGGAGGAATCGTTTCAGCAGTTGAGAATGCTTTTCATGTCACCCCTTCTTTAGCATGTTCAGAGGGTGCACTGCAGGAACTTCGTTTATGTTTCTACAAGGATCTCAAGCCTCGTGACTGTGTTCAGACAGACTCACAGAATGGGATCATTTCTTCAAATTTTTCGTGTCCCCAATATGTGAGCTTACCAGCTATTGATCCTAGTGGCAATATTTTCAACTCTCCTTTTGTACAAAGTACCCGGATTTTATTTAGGAGAAAGCAAAAAAAGGAACGGAGTTGCACCTTTTACATGGGGTTTTGTCCTGCAATTGTTGGTACAAAATTTCACCTACAAGATATGTTATGA

Protein sequence

MIPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCSSSTCHEKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFSCPQYVSLPAIDPSGNIFNSPFVQSTRILFRRKQKKERSCTFYMGFCPAIVGTKFHLQDML

Homology

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BLAST of CmaCh17G013260 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.2e-79
Identity = 135/224 (60.27%), Postives = 162/224 (72.32%), Query Frame = 0


Query: 30  IKDGREQRELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFADFTIHGLWPDYNDGTW 89
           I+  R QRE DYF L+LQWPGT C+GT  CCS NACC  S     FTIHGLWPDYNDG+W
Sbjct: 23  IELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSW 82

Query: 90  PSCCNGTSFDEKEIHTLLDPLKKYWPSLSCSS-STCH-----------EKHGTCSYPVIR 149
           PSCC  + F EKEI TL+D L+KYWPSLSC S S+C+           EKHGTCS PV  
Sbjct: 83  PSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFH 142

Query: 150 DEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACSEGA 209
           DEY+YFLTTLN++ K+NVT VL  AGY+ SN+EKYPLGGIV+A++NAFH+TP + C   A
Sbjct: 143 DEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDA 202

Query: 210 LQELRLCFYKDLKPRDCVQTDSQNGIISSNFSCPQYVSLPAIDP 242
           + E+R+CFYKD KPRDCV +      ++S  SCP+YVSLP   P
Sbjct: 203 IDEIRICFYKDFKPRDCVGSQD----LTSRKSCPKYVSLPEYTP 242

[Close]

BLAST of CmaCh17G013260 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.1e-25
Identity = 65/165 (39.39%), Postives = 88/165 (53.33%), Query Frame = 0


Query: 38  ELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTS 97
           + D+F    QWPG+ C     CC  N+        ADF IHGLWP+Y DGT+PS C+ + 
Sbjct: 29  DFDFFYFVQQWPGSYCDTQKKCCYPNS----GKPAADFGIHGLWPNYKDGTYPSNCDASK 88

Query: 98  -FDEKEIHTLLDPLKKYWPSLSCSSSTCH-------EKHGTCSYPVIRDEYSYFLTTLNV 157
            FD   I  LL  +KK WP+L+C S +         EKHGTCS  VI D++ YF T LN+
Sbjct: 89  PFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVI-DQHEYFQTALNL 148

Query: 158 FFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACS 195
             K N+   L  AG  P   + Y L  I  +++ +   TP + C+
Sbjct: 149 KQKTNLLGALTKAGINPDG-KSYSLESIRDSIKESIGFTPWVECN 187

[Close]

BLAST of CmaCh17G013260 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 4.4e-25
Identity = 63/166 (37.95%), Postives = 88/166 (53.01%), Query Frame = 0


Query: 37  RELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFADFTIHGLWPDYNDGTWP-SCCNG 96
           ++ D+F    QWP + C    SCC             DF+IHGLWP+Y DG WP +C   
Sbjct: 26  QDFDFFYFVQQWPASYCDTRRSCCYPTT----GKPDEDFSIHGLWPNYKDGKWPQNCDRE 85

Query: 97  TSFDEKEIHTLLDPLKKYWPSLSCSSST-----CHE--KHGTCSYPVIRDEYSYFLTTLN 156
           +S DE E   L+  ++K WPSL+C SS       HE  KHGTCS     ++++YF T L+
Sbjct: 86  SSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTCS---ALNQHAYFQTALD 145

Query: 157 VFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACS 195
              K N+ + LN+AG  P N + Y +  I  A+E     TP + C+
Sbjct: 146 FKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIECN 184

[Close]

BLAST of CmaCh17G013260 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.1e-23
Identity = 58/166 (34.94%), Postives = 93/166 (56.02%), Query Frame = 0


Query: 37  RELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGT 96
           ++ D+F    QWPG+ C    SCC            ADF IHGLWP+ NDGT+PS C+  
Sbjct: 27  KDFDFFYFVQQWPGSYCDTKQSCCYPTT----GKPAADFGIHGLWPNNNDGTYPSNCDPN 86

Query: 97  S-FDEKEIHTLLDPLKKYWPSLSCSSST-----CH--EKHGTCSYPVIRDEYSYFLTTLN 156
           S +D+ +I  L+  +++ WP+L+C S +      H  EKHGTC+  V+ ++++YF   L+
Sbjct: 87  SPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVLTNQHAYFKKALD 146

Query: 157 VFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACS 195
           +  + ++  +L  A   P   E Y L  I +A+++A   TP + C+
Sbjct: 147 LKNQIDLLSILQGADIHPDG-ESYDLVNIRNAIKSAIGYTPWIQCN 187

[Close]

BLAST of CmaCh17G013260 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 107.8 bits (268), Expect = 2.0e-22
Identity = 71/207 (34.30%), Postives = 101/207 (48.79%), Query Frame = 0


Query: 14  VASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFA 73
           +AST+   S+  I    K G    + D++    QW         S C S  C       A
Sbjct: 13  IASTA--QSTVTIYESSKPG----DFDFYLFVQQW-------IYSYCDSQTCIQNKEREA 72

Query: 74  DFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCSSST--CHE--KHG 133
            FTIHGLWP+ +DG++PS C+G SF+   I  L D L   WPSL+  ++    HE  KHG
Sbjct: 73  -FTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDWPSLTGPNTDFWTHEFSKHG 132

Query: 134 TCSYP-VIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVT 193
           TCS    I D + YF T + ++ ++N+T  L      PS++  Y    I +A+   F   
Sbjct: 133 TCSITGPITDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDITNAITTHFGGK 192

Query: 194 PSLACSEGALQELRLCFYKD-LKPRDC 215
           P + CS G L  + +C  K+ L   DC
Sbjct: 193 PGIQCSSGQLSTVAVCIDKNSLSIMDC 205

[Close]

BLAST of CmaCh17G013260 vs. ExPASy TrEMBL
Match: A0A6J1JVW6 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111488355 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 6.3e-144
Identity = 250/266 (93.98%), Postives = 251/266 (94.36%), Query Frame = 0


Query: 1   MIPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCC 60
           MIPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCC
Sbjct: 1   MIPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCC 60

Query: 61  SSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCS 120
           SSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCS
Sbjct: 61  SSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCS 120

Query: 121 SSTCH-----------EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNT 180
           SSTCH           EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNT
Sbjct: 121 SSTCHGGKGSFWAHEWEKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNT 180

Query: 181 EKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFS 240
           EKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFS
Sbjct: 181 EKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFS 240

Query: 241 CPQYVSLPAIDPSGNIFNSPFVQSTR 256
           CPQYVSLPAIDPSGNIF   FV S+R
Sbjct: 241 CPQYVSLPAIDPSGNIFK--FVDSSR 264

[Close]

BLAST of CmaCh17G013260 vs. ExPASy TrEMBL
Match: A0A6J1GMH3 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111455791 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.4e-137
Identity = 242/268 (90.30%), Postives = 247/268 (92.16%), Query Frame = 0


Query: 1   MIPFIFLLQFTVSVASTSLNDSS-DAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSC 60
           MIPFIF LQF +SVASTSLNDSS DAIISQIK+GREQRELDYFKLTLQWPGTVCKGTDSC
Sbjct: 1   MIPFIFFLQFALSVASTSLNDSSADAIISQIKEGREQRELDYFKLTLQWPGTVCKGTDSC 60

Query: 61  CSSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSC 120
           CSSNACCNRSG FADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIH+LLDPLKKYWPSLSC
Sbjct: 61  CSSNACCNRSGAFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHSLLDPLKKYWPSLSC 120

Query: 121 SSSTCH-----------EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN 180
           SSSTCH           EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN
Sbjct: 121 SSSTCHGGKGSFWAHEWEKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN 180

Query: 181 TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNF 240
           TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNF
Sbjct: 181 TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNF 240

Query: 241 SCPQYVSLPAIDPSG-NIFNSPFVQSTR 256
           SCPQYVSLPA +PSG NIF   FV S+R
Sbjct: 241 SCPQYVSLPAYEPSGNNIFK--FVDSSR 266

[Close]

BLAST of CmaCh17G013260 vs. ExPASy TrEMBL
Match: A0A6J1GMN1 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111455791 PE=3 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 4.3e-116
Identity = 213/257 (82.88%), Postives = 221/257 (85.99%), Query Frame = 0


Query: 1   MIPFIFLLQFTVSVASTSLNDSS-DAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSC 60
           MIPFIF LQF +SVASTSLNDSS DAIISQIK+GREQRELDYFKLTLQWPGTVCKGTDSC
Sbjct: 1   MIPFIFFLQFALSVASTSLNDSSADAIISQIKEGREQRELDYFKLTLQWPGTVCKGTDSC 60

Query: 61  CSSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSC 120
           CSSNACCNRSG FADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIH+LLDPLKKYWPSLSC
Sbjct: 61  CSSNACCNRSGAFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHSLLDPLKKYWPSLSC 120

Query: 121 SSSTCHEKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNTEKYPLGGIVS 180
           SSSTCH   G+                   F+ +   KVLNDAGYLPSNTEKYPLGGIVS
Sbjct: 121 SSSTCHGGKGS-------------------FWAHE--KVLNDAGYLPSNTEKYPLGGIVS 180

Query: 181 AVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFSCPQYVSLPAI 240
           AVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFSCPQYVSLPA 
Sbjct: 181 AVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFSCPQYVSLPAY 234

Query: 241 DPSG-NIFNSPFVQSTR 256
           +PSG NIF   FV S+R
Sbjct: 241 EPSGNNIFK--FVDSSR 234

[Close]

BLAST of CmaCh17G013260 vs. ExPASy TrEMBL
Match: A0A6J1H611 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111460790 PE=3 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.3e-114
Identity = 195/257 (75.88%), Postives = 216/257 (84.05%), Query Frame = 0


Query: 2   IPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCCS 61
           I FIFLLQF++SVASTSL ++SDA+I Q + GR+QRE DYF L LQWPGT+CKGT  CCS
Sbjct: 14  ILFIFLLQFSLSVASTSLKEASDAVILQNQGGRKQREFDYFVLALQWPGTICKGTRHCCS 73

Query: 62  SNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCSS 121
           SNACCNRSGGFA+FTIHGLWPDYNDGTWPSCC G  FDEKEIHTLL+P+KKYW SLSCSS
Sbjct: 74  SNACCNRSGGFAEFTIHGLWPDYNDGTWPSCCTGKEFDEKEIHTLLEPMKKYWSSLSCSS 133

Query: 122 -STCH-----------EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNT 181
             TCH           EKHGTCS PV RDEY YFLTTLNV+FKYNVTKVLNDAGY+PSNT
Sbjct: 134 PKTCHGAKGSFWAHEWEKHGTCSSPVARDEYGYFLTTLNVYFKYNVTKVLNDAGYVPSNT 193

Query: 182 EKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFS 241
           EKYPLGGI+SAVENAFH TP L CS+GA++ELRLCFYKD KPRDCV TDSQN I+SS  S
Sbjct: 194 EKYPLGGIISAVENAFHATPLLVCSKGAVEELRLCFYKDFKPRDCVPTDSQNAIVSSKIS 253

Query: 242 CPQYVSLPAIDPSGNIF 247
           CP+YVSLP+ +P G IF
Sbjct: 254 CPKYVSLPSYEPGGKIF 270

[Close]

BLAST of CmaCh17G013260 vs. ExPASy TrEMBL
Match: A0A6J1KXE3 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111498005 PE=3 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.2e-113
Identity = 193/257 (75.10%), Postives = 216/257 (84.05%), Query Frame = 0


Query: 2   IPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCCS 61
           I FIFLLQF++SVASTSLN++SDA+I Q + GR+QRE DYF L LQWPGT+CKGT  CCS
Sbjct: 14  ILFIFLLQFSLSVASTSLNEASDAVILQNQGGRKQREFDYFVLALQWPGTICKGTRHCCS 73

Query: 62  SNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCSS 121
           SNACCNRSGGF++FTIHGLWPDYNDGTWPSCC G  FDEKEIHTLL+P+KKYW SLSC S
Sbjct: 74  SNACCNRSGGFSEFTIHGLWPDYNDGTWPSCCTGKEFDEKEIHTLLEPMKKYWSSLSCGS 133

Query: 122 -STCH-----------EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNT 181
            STCH           EKHGTCS PV  DEY YFLTTLNV+FKYNVTKVLN+AGY+PSNT
Sbjct: 134 PSTCHGAKGSFWAHEWEKHGTCSSPVAHDEYGYFLTTLNVYFKYNVTKVLNEAGYVPSNT 193

Query: 182 EKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFS 241
           EKYPLGGI+SAVENAFH TP L CS+GA++ELRLCFYKD KPRDCV TDSQN I+SS  S
Sbjct: 194 EKYPLGGIISAVENAFHATPLLVCSKGAVEELRLCFYKDFKPRDCVPTDSQNAIVSSKIS 253

Query: 242 CPQYVSLPAIDPSGNIF 247
           CP+YVSLP+ +P G IF
Sbjct: 254 CPKYVSLPSYEPGGKIF 270

[Close]

BLAST of CmaCh17G013260 vs. NCBI nr
Match: XP_022991824.1 (ribonuclease 2-like [Cucurbita maxima])

HSP 1 Score: 520.0 bits (1338), Expect = 1.3e-143
Identity = 250/266 (93.98%), Postives = 251/266 (94.36%), Query Frame = 0


Query: 1   MIPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCC 60
           MIPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCC
Sbjct: 1   MIPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCC 60

Query: 61  SSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCS 120
           SSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCS
Sbjct: 61  SSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCS 120

Query: 121 SSTCH-----------EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNT 180
           SSTCH           EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNT
Sbjct: 121 SSTCHGGKGSFWAHEWEKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNT 180

Query: 181 EKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFS 240
           EKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFS
Sbjct: 181 EKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFS 240

Query: 241 CPQYVSLPAIDPSGNIFNSPFVQSTR 256
           CPQYVSLPAIDPSGNIF   FV S+R
Sbjct: 241 CPQYVSLPAIDPSGNIFK--FVDSSR 264

[Close]

BLAST of CmaCh17G013260 vs. NCBI nr
Match: XP_023547521.1 (ribonuclease 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500.0 bits (1286), Expect = 1.4e-137
Identity = 239/258 (92.64%), Postives = 243/258 (94.19%), Query Frame = 0


Query: 1   MIPFIFLLQFTVSVASTSLNDSS-DAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSC 60
           MIPFIFLLQFT+SVAST LNDSS DAIISQIK+GREQRELDYFKLTLQWPGTVCKGTDSC
Sbjct: 1   MIPFIFLLQFTLSVASTPLNDSSADAIISQIKEGREQRELDYFKLTLQWPGTVCKGTDSC 60

Query: 61  CSSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSC 120
           CSSNACCNRSG FADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSC
Sbjct: 61  CSSNACCNRSGAFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSC 120

Query: 121 SSSTCH-----------EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN 180
           SSSTCH           EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN
Sbjct: 121 SSSTCHGGKGSFWAHEWEKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN 180

Query: 181 TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNF 240
           TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQ+GIISSNF
Sbjct: 181 TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQSGIISSNF 240

Query: 241 SCPQYVSLPAIDPSGNIF 247
           SCPQYVSLPA +PSGNIF
Sbjct: 241 SCPQYVSLPAYEPSGNIF 258

[Close]

BLAST of CmaCh17G013260 vs. NCBI nr
Match: XP_022953172.1 (ribonuclease 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 497.3 bits (1279), Expect = 9.1e-137
Identity = 242/268 (90.30%), Postives = 247/268 (92.16%), Query Frame = 0


Query: 1   MIPFIFLLQFTVSVASTSLNDSS-DAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSC 60
           MIPFIF LQF +SVASTSLNDSS DAIISQIK+GREQRELDYFKLTLQWPGTVCKGTDSC
Sbjct: 1   MIPFIFFLQFALSVASTSLNDSSADAIISQIKEGREQRELDYFKLTLQWPGTVCKGTDSC 60

Query: 61  CSSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSC 120
           CSSNACCNRSG FADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIH+LLDPLKKYWPSLSC
Sbjct: 61  CSSNACCNRSGAFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHSLLDPLKKYWPSLSC 120

Query: 121 SSSTCH-----------EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN 180
           SSSTCH           EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN
Sbjct: 121 SSSTCHGGKGSFWAHEWEKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN 180

Query: 181 TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNF 240
           TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNF
Sbjct: 181 TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNF 240

Query: 241 SCPQYVSLPAIDPSG-NIFNSPFVQSTR 256
           SCPQYVSLPA +PSG NIF   FV S+R
Sbjct: 241 SCPQYVSLPAYEPSGNNIFK--FVDSSR 266

[Close]

BLAST of CmaCh17G013260 vs. NCBI nr
Match: KAG6576042.1 (Ribonuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 491.9 bits (1265), Expect = 3.8e-135
Identity = 232/245 (94.69%), Postives = 238/245 (97.14%), Query Frame = 0


Query: 12  VSVASTSLNDSS-DAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSG 71
           +SVASTSLNDSS DAIISQIK+GREQRELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSG
Sbjct: 244 LSVASTSLNDSSADAIISQIKEGREQRELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSG 303

Query: 72  GFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCSSSTCHEKHGT 131
            FADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIH+LLDPLKKYWPSLSCSSSTCHEKHGT
Sbjct: 304 AFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHSLLDPLKKYWPSLSCSSSTCHEKHGT 363

Query: 132 CSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPS 191
           CSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPS
Sbjct: 364 CSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPS 423

Query: 192 LACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNFSCPQYVSLPAIDPSGNIFNSPF 251
           LACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISS+FSCPQYVSLPA +PSGNIF   F
Sbjct: 424 LACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSDFSCPQYVSLPAYEPSGNIFK--F 483

Query: 252 VQSTR 256
           V S+R
Sbjct: 484 VDSSR 486

[Close]

BLAST of CmaCh17G013260 vs. NCBI nr
Match: XP_038900059.1 (ribonuclease 2-like [Benincasa hispida])

HSP 1 Score: 434.9 bits (1117), Expect = 5.5e-118
Identity = 203/267 (76.03%), Postives = 222/267 (83.15%), Query Frame = 0


Query: 1   MIPFIFLLQFTVSVASTSLNDSSDAIISQIKDGREQRELDYFKLTLQWPGTVCKGTDSCC 60
           ++PFIFLLQF++S+ASTSLN  SDAII  IKDGR+QRE DYFKL LQWPGT+CK T  CC
Sbjct: 6   VVPFIFLLQFSLSLASTSLNQPSDAIIFPIKDGRQQREFDYFKLALQWPGTICKATRHCC 65

Query: 61  SSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTSFDEKEIHTLLDPLKKYWPSLSCS 120
           SSNACCNRSG FADFTIHGLWPDYNDGTWP+CC G  F+EKEIHTLLDPLKKYWPSLSC+
Sbjct: 66  SSNACCNRSGAFADFTIHGLWPDYNDGTWPACCAGKKFNEKEIHTLLDPLKKYWPSLSCN 125

Query: 121 S-STCH-----------EKHGTCSYPVIRDEYSYFLTTLNVFFKYNVTKVLNDAGYLPSN 180
           S STCH           EKHGTCSYPV RDEYSYFLTTLNV+FKYNVTKVLNDAGY+PSN
Sbjct: 126 SPSTCHGAKGSFWAHEWEKHGTCSYPVARDEYSYFLTTLNVYFKYNVTKVLNDAGYVPSN 185

Query: 181 TEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKDLKPRDCVQTDSQNGIISSNF 240
           TEKYPLGGI+SA++NAFH TPSL C  GA++EL LCFYKD KPRDC QT SQNGIISS F
Sbjct: 186 TEKYPLGGIISAIQNAFHATPSLECKNGAVEELYLCFYKDFKPRDCAQTHSQNGIISSKF 245

Query: 241 SCPQYVSLPAIDPSGNIFNSPFVQSTR 256
           SCP+YVSLPA  P   IF   F  S+R
Sbjct: 246 SCPKYVSLPAYKPEAKIFK--FTHSSR 270

[Close]

BLAST of CmaCh17G013260 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 295.8 bits (756), Expect = 3.7e-80
Identity = 135/224 (60.27%), Postives = 162/224 (72.32%), Query Frame = 0


Query: 30  IKDGREQRELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFADFTIHGLWPDYNDGTW 89
           I+  R QRE DYF L+LQWPGT C+GT  CCS NACC  S     FTIHGLWPDYNDG+W
Sbjct: 23  IELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSW 82

Query: 90  PSCCNGTSFDEKEIHTLLDPLKKYWPSLSCSS-STCH-----------EKHGTCSYPVIR 149
           PSCC  + F EKEI TL+D L+KYWPSLSC S S+C+           EKHGTCS PV  
Sbjct: 83  PSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFH 142

Query: 150 DEYSYFLTTLNVFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACSEGA 209
           DEY+YFLTTLN++ K+NVT VL  AGY+ SN+EKYPLGGIV+A++NAFH+TP + C   A
Sbjct: 143 DEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDA 202

Query: 210 LQELRLCFYKDLKPRDCVQTDSQNGIISSNFSCPQYVSLPAIDP 242
           + E+R+CFYKD KPRDCV +      ++S  SCP+YVSLP   P
Sbjct: 203 IDEIRICFYKDFKPRDCVGSQD----LTSRKSCPKYVSLPEYTP 242

[Close]

BLAST of CmaCh17G013260 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 240.4 bits (612), Expect = 1.9e-63
Identity = 113/213 (53.05%), Postives = 138/213 (64.79%), Query Frame = 0


Query: 30  IKDGREQRELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFADFTIHGLWPDYNDGTW 89
           I+  R QRE DYF L+LQWPGT C+GT  CCS NACC  S     FTIHGLWPDYNDG+W
Sbjct: 23  IELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSW 82

Query: 90  PSCCNGTSFDEKEIHTLLDPLKKYWPSLSCSS-STCHEKHGTCSYPVIRDEYSYFLTTLN 149
           PSCC  + F EKEI TL+D L+KYWPSLSC S S+C+   G+                  
Sbjct: 83  PSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSF----------------- 142

Query: 150 VFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACSEGALQELRLCFYKD 209
               +    VL  AGY+ SN+EKYPLGGIV+A++NAFH+TP + C   A+ E+R+CFYKD
Sbjct: 143 ----WGHEDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDAIDEIRICFYKD 202

Query: 210 LKPRDCVQTDSQNGIISSNFSCPQYVSLPAIDP 242
            KPRDCV +      ++S  SCP+YVSLP   P
Sbjct: 203 FKPRDCVGSQD----LTSRKSCPKYVSLPEYTP 210

[Close]

BLAST of CmaCh17G013260 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 125.9 bits (315), Expect = 5.1e-29
Identity = 69/172 (40.12%), Postives = 97/172 (56.40%), Query Frame = 0


Query: 34  REQRELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGG--FADFTIHGLWPDYNDGTWPS 93
           R Q + D+F   LQWPG  C       +S ACC  + G   ADF IHGLWP+YN G+WPS
Sbjct: 23  RPQEDFDFFYFVLQWPGAYCD------TSRACCYPTSGKPAADFGIHGLWPNYNGGSWPS 82

Query: 94  CCNGTS-FDEKEIHTLLDPLKKYWPSLSCSSSTCH-------EKHGTCSYPVIRDEYSYF 153
            C+  S FD  +I  L+  LKK WP+LSC S+          EKHGTCS  V+ D++ YF
Sbjct: 83  NCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVM-DQHEYF 142

Query: 154 LTTLNVFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACSE 196
              L +  K N+ ++L ++G  P +   Y L  I +A+++    TP + C++
Sbjct: 143 ENALKLKQKANLLQILKNSGINPDD-GFYNLDKITNAIKDGIGFTPGIECNK 186

[Close]

BLAST of CmaCh17G013260 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 118.6 bits (296), Expect = 8.2e-27
Identity = 65/165 (39.39%), Postives = 88/165 (53.33%), Query Frame = 0


Query: 38  ELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGTS 97
           + D+F    QWPG+ C     CC  N+        ADF IHGLWP+Y DGT+PS C+ + 
Sbjct: 29  DFDFFYFVQQWPGSYCDTQKKCCYPNS----GKPAADFGIHGLWPNYKDGTYPSNCDASK 88

Query: 98  -FDEKEIHTLLDPLKKYWPSLSCSSSTCH-------EKHGTCSYPVIRDEYSYFLTTLNV 157
            FD   I  LL  +KK WP+L+C S +         EKHGTCS  VI D++ YF T LN+
Sbjct: 89  PFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVI-DQHEYFQTALNL 148

Query: 158 FFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACS 195
             K N+   L  AG  P   + Y L  I  +++ +   TP + C+
Sbjct: 149 KQKTNLLGALTKAGINPDG-KSYSLESIRDSIKESIGFTPWVECN 187

[Close]

BLAST of CmaCh17G013260 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-23
Identity = 61/181 (33.70%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 37  RELDYFKLTLQWPGTVCKGTDSCCSSNACCNRSGGFADFTIHGLWPDYNDGTWPSCCNGT 96
           ++ D+F   LQWPG  C    SCC            ADF IHGLWP+Y  G WP  CN  
Sbjct: 20  QDFDFFYFVLQWPGAYCDSRHSCCYPQT----GKPAADFGIHGLWPNYKTGGWPQNCNPD 79

Query: 97  S-FDEKEIHTLLDPLKKYWPSLSCSSST-----CH--EKHGTCSYPVIRDEYSYFLTTLN 156
           S FD+  +  L+  L++ WP+LSC S+       H  EKHGTC+   + D++ YF   L 
Sbjct: 80  SRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESEL-DQHDYFEAGLK 139

Query: 157 VFFKYNVTKVLNDAGYLPSNTEKYPLGGIVSAVENAFHVTPSLACSEGA-----LQELRL 205
           +  K N+   L +AG  P + + Y +  I + ++      P + C++ +     L ++ L
Sbjct: 140 LKQKANLLHALTNAGIKPDD-KFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYL 194

The following BLAST results are available for this feature:

BLAST of CmaCh17G013260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42814	5.2e-79	60.27	Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1	[more]
P42813	1.1e-25	39.39	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80196	4.4e-25	37.95	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P80022	1.1e-23	34.94	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
Q7M438	2.0e-22	34.30	Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3	[more]

BLAST of CmaCh17G013260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JVW6	6.3e-144	93.98	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111488355 PE=3 SV=1	[more]
A0A6J1GMH3	4.4e-137	90.30	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111455791 PE=3 SV=1	[more]
A0A6J1GMN1	4.3e-116	82.88	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111455791 PE=3 SV=1	[more]
A0A6J1H611	2.3e-114	75.88	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111460790 PE=3 SV=1	[more]
A0A6J1KXE3	1.2e-113	75.10	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111498005 PE=3 SV=1	[more]

BLAST of CmaCh17G013260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022991824.1	1.3e-143	93.98	ribonuclease 2-like [Cucurbita maxima]	[more]
XP_023547521.1	1.4e-137	92.64	ribonuclease 2-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022953172.1	9.1e-137	90.30	ribonuclease 2-like isoform X1 [Cucurbita moschata]	[more]
KAG6576042.1	3.8e-135	94.69	Ribonuclease 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038900059.1	5.5e-118	76.03	ribonuclease 2-like [Benincasa hispida]	[more]

BLAST of CmaCh17G013260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39780.1	3.7e-80	60.27	ribonuclease 2	[more]
AT2G39780.2	1.9e-63	53.05	ribonuclease 2	[more]
AT1G14220.1	5.1e-29	40.12	Ribonuclease T2 family protein	[more]
AT2G02990.1	8.2e-27	39.39	ribonuclease 1	[more]
AT1G26820.1	1.4e-23	33.70	ribonuclease 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 40..214 e-value: 3.1E-41 score: 141.6
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 19..244
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 35..240 e-value: 8.9E-60 score: 204.2
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 38..236
None	No IPR available	PANTHER	PTHR11240:SF53	RIBONUCLEASE 2-LIKE ISOFORM X1	coord: 19..244
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 75..82
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 40..221 e-value: 1.85067E-56 score: 178.68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh17G013260.1	CmaCh17G013260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding