CmaCh17G012480 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G012480
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description3-methyl-2-oxobutanoate hydroxymethyltransferase
LocationCma_Chr17: 8531205 .. 8534227 (-)
RNA-Seq ExpressionCmaCh17G012480
SyntenyCmaCh17G012480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCAACTAATTCGCAGGCAACAGAGCAACCAACCGATTGTGAGAATCCATTTCTTTCGAAGACAAATTTCTGTCCATCAATGGCGCTCTTCAACCTCGTCACCAAGGCCGCAGTTCGTTCCACATCGCAATCACTGCTCAACACGCTTCGTTACATGAGCAATGTCCCTGAAAACACGGTCTACGGCGGCCCGACATCGAACACTCCAAACCAGAGAGTCACTCTCACCAATCTGAAACAAAAGTACAAGAAAGGCGAACCAATCACCATGGTCACTGCTTACGATTACCCCTCCGGGGTCCATTTGGATATGGCCGGCATTGATATTGCTTTGGTTGGCGATTCGGCTTCCATGGTAGTACACGGCCACGACACCACGTTGCCTATTACCTTGGATGAAATGCTTGTTCATTGCAGGGCTGTGGCTCGTGGGGCTAGGAGGCCGCTTTTGATCGGCGACTTGCCCTTTGGTACCTACGAATCCAGCTCCGTTCAGGTTATTTTCCTTTTGCCTCTTAGGTGTTCGATGAAATGATAGCCATCTGGATTTATTCAAAAATCTAATTTCCATGCTTAGAGATGGGCCGCACGGGAATCTAAGAAATTCATTTTTCTTTTTTCATGCTAGATTGTAAAAGGTAAAAGCAGTTTCAAGCTCTTGTTAGCATTATCGAAAGGATTGAAAGTGGGAGGTCTTAAATTCAAATCTAAACATGTTTCCATTTGATTTCTTAGTTAAATTAATCACCATCATGTCTCAAAATGCACGAACAGACAGAACTTAACCTTTTTGTAGTTCATCTTCTTTTGATAGATTCTGTTATGCTATTGTTTTTATGCCAACTCCTTTTTAGGATGCTAAAACCTTAAACAAATCAATACCCACTTGACGAAAGAATCATAGACGACATCTGAATTCTCAAAGTTATTCAGAACAGTGTTGCTTACTATTGTCAGGCTGTTGATACGGCAGTACGAGTTTTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCAGGAATTGCGGTAATGGGGCACGTTGGGCTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAACATCGCCAGTGCTGTTAAGGTTCCCCTCACTCCCCTACTTCCCTCTTCTTATTAGTACTGTTTGCAGTTGTTTCTGGGATACAATTTCTGTTGTTATGTTGCATAGCTTGTCGTGTTCAGTTTGGCTGCAAAATGCTGAATAGTGTATCTTTTGCAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTCGCCGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCAGCTGCTGCTACAGCTGCTCTCCGCATTCCCACCATAGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTTAGTTGATTGCACAGACATCCTTTTACATGCCATATGCACATACACACGTACATACATATAGGGCTGCCAACCGTATTTCCTTTCCTTGAGTTATTTGTTAGCTTCTCTTTGTTTGACCTTTGCATATAATTATTCTTTCAAACTATTTCTTTTTCCCCGAGTTCAGGACTCGGAGTTTCTTTCTCGAGTTCTCAATTAGAGAAGCATCGAAGTTCATCCTTGTTTAGCTTTCCTATATGGTCCTGTTCTATGGTTATTCGATTCTTGCACCGTGATATCCGAAGAGACTGTAAGTGTTTAAATTGTTGTGAAAATCCCTAGTGGTTCATTGTGCTAGACTGAACTGCAACTGTTTAACTTTAAATTGTTTCGAGAATCCCTAAGTAGTGTATGCATCAGAACATAGAGGACATATTCAAGATTAGTGAAATATGTCTACTTGGTAACAAGTCATGGTGGCAAAATAGCGATTTGTTAATATTTGGGTTCAGACGCCGCTCCCTCGAATTGAAAGGAGCTTAAGAAGAAGAAAATAGACCTTGCATTTCACTAAAAAAAGAGACGGGGTTTATGATTTATTCCATATTTCAGATAACTTGTTAGTTTCTCTTTAAAGAAATGTTGGTTTCATCATCCATACTTGTTTGATAAGCTTGTCCATTGTGCTGCTCGTTTTGTCTTTGATTACTTGACGACAATCATAGAGTTGAATATCCTCTGCAGCTGGACTAACTAATTTCTTAAACACAGGTGCTAGTTTACCATGATCTACTCGGCATGCTTCAACACCCGCATCATGCTAAGGTTACCATCAGCCCGCACCTTTGTTTCAACTAAGTTTCTCTATGTTTGTTTAAGGTGTTTTGACTCCTGGATTGGATGTGCCATTGCAGGTGACCCCGAAATTCTGCAAGCAATTTGCACAAATTGGAGATGTCATCAACAAAGCTTTATCAGATTACAAGGAAGAGGTATCTAATGGTTCATTTCCCGGACCGCTCCATAGCCCATATAAGATCAGCGACAACGATTACAACGGTTTCTTGAAAGAGTTACGAAGTATGGGATTGGATGGGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCAATGATTAAACTCAGCTGCATTCACTGCCTCGCCCTCTTGGTAGTCTAGCCTTTCGAGTTTTAGATCATTTATGGAATTTATCAGTTGGGACAATTCGTTCTTAGAATCATTAGCTGAAGAACACTTGAACCACTAGAACACTATTATCCCCTTTAGTTATAGCTGATATGAAGATGTGTTTGGATTCTGAACAATTAGAAGGCACCAAGCCACTGCCACTGCCACTGCCTCAGCCACCGGATTCTGAACAATAAAAAGGATCTATCTTTTTGGTATTAGCATCTCAAATTTTCCAGGTGCAATTTAGCTTTTCGTGTGGGACGCCACGAATGTTCTTAAAGCTGGAATAAAGATTACTTTTATTTTCGTTTTAAATACTCCATTTTGTAGCATTTGGCTGTGTTCAGTAAGATATTAATTGTATTTTATTTTTAAAAAAATTAATTTGTTTTTTATTACTCGG

mRNA sequence

TGCAACTAATTCGCAGGCAACAGAGCAACCAACCGATTGTGAGAATCCATTTCTTTCGAAGACAAATTTCTGTCCATCAATGGCGCTCTTCAACCTCGTCACCAAGGCCGCAGTTCGTTCCACATCGCAATCACTGCTCAACACGCTTCGTTACATGAGCAATGTCCCTGAAAACACGGTCTACGGCGGCCCGACATCGAACACTCCAAACCAGAGAGTCACTCTCACCAATCTGAAACAAAAGTACAAGAAAGGCGAACCAATCACCATGGTCACTGCTTACGATTACCCCTCCGGGGTCCATTTGGATATGGCCGGCATTGATATTGCTTTGGTTGGCGATTCGGCTTCCATGGTAGTACACGGCCACGACACCACGTTGCCTATTACCTTGGATGAAATGCTTGTTCATTGCAGGGCTGTGGCTCGTGGGGCTAGGAGGCCGCTTTTGATCGGCGACTTGCCCTTTGGTACCTACGAATCCAGCTCCGTTCAGGCTGTTGATACGGCAGTACGAGTTTTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCAGGAATTGCGGTAATGGGGCACGTTGGGCTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAACATCGCCAGTGCTGTTAAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTCGCCGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCAGCTGCTGCTACAGCTGCTCTCCGCATTCCCACCATAGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGCTAGTTTACCATGATCTACTCGGCATGCTTCAACACCCGCATCATGCTAAGGTGACCCCGAAATTCTGCAAGCAATTTGCACAAATTGGAGATGTCATCAACAAAGCTTTATCAGATTACAAGGAAGAGGTATCTAATGGTTCATTTCCCGGACCGCTCCATAGCCCATATAAGATCAGCGACAACGATTACAACGGTTTCTTGAAAGAGTTACGAAGTATGGGATTGGATGGGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCAATGATTAAACTCAGCTGCATTCACTGCCTCGCCCTCTTGGTAGTCTAGCCTTTCGAGTTTTAGATCATTTATGGAATTTATCAGTTGGGACAATTCGTTCTTAGAATCATTAGCTGAAGAACACTTGAACCACTAGAACACTATTATCCCCTTTAGTTATAGCTGATATGAAGATGTGTTTGGATTCTGAACAATTAGAAGGCACCAAGCCACTGCCACTGCCACTGCCTCAGCCACCGGATTCTGAACAATAAAAAGGATCTATCTTTTTGGTATTAGCATCTCAAATTTTCCAGGTGCAATTTAGCTTTTCGTGTGGGACGCCACGAATGTTCTTAAAGCTGGAATAAAGATTACTTTTATTTTCGTTTTAAATACTCCATTTTGTAGCATTTGGCTGTGTTCAGTAAGATATTAATTGTATTTTATTTTTAAAAAAATTAATTTGTTTTTTATTACTCGG

Coding sequence (CDS)

ATGGCGCTCTTCAACCTCGTCACCAAGGCCGCAGTTCGTTCCACATCGCAATCACTGCTCAACACGCTTCGTTACATGAGCAATGTCCCTGAAAACACGGTCTACGGCGGCCCGACATCGAACACTCCAAACCAGAGAGTCACTCTCACCAATCTGAAACAAAAGTACAAGAAAGGCGAACCAATCACCATGGTCACTGCTTACGATTACCCCTCCGGGGTCCATTTGGATATGGCCGGCATTGATATTGCTTTGGTTGGCGATTCGGCTTCCATGGTAGTACACGGCCACGACACCACGTTGCCTATTACCTTGGATGAAATGCTTGTTCATTGCAGGGCTGTGGCTCGTGGGGCTAGGAGGCCGCTTTTGATCGGCGACTTGCCCTTTGGTACCTACGAATCCAGCTCCGTTCAGGCTGTTGATACGGCAGTACGAGTTTTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCAGGAATTGCGGTAATGGGGCACGTTGGGCTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAACATCGCCAGTGCTGTTAAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTCGCCGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCAGCTGCTGCTACAGCTGCTCTCCGCATTCCCACCATAGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTGCTAGTTTACCATGATCTACTCGGCATGCTTCAACACCCGCATCATGCTAAGGTGACCCCGAAATTCTGCAAGCAATTTGCACAAATTGGAGATGTCATCAACAAAGCTTTATCAGATTACAAGGAAGAGGTATCTAATGGTTCATTTCCCGGACCGCTCCATAGCCCATATAAGATCAGCGACAACGATTACAACGGTTTCTTGAAAGAGTTACGAAGTATGGGATTGGATGGGGCAGCATCTGCAGCTGCTGCTAAAGCCAACCAATGA

Protein sequence

MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGARRPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ
Homology
[Close]
BLAST of CmaCh17G012480 vs. ExPASy Swiss-Prot
Match: Q9M315 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT2 PE=1 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 1.5e-152
Identity = 269/334 (80.54%), Postives = 303/334 (90.72%), Query Frame = 0 

Query: 14  STSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSG 73
           S +   + T+R+MSNVPE+TVYGGP     NQRVTLT L+QK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPI+L+EMLVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVRVLKEGGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPF 253
           GGFRPQGKNIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPTIGIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKAL +YKEEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           PYKIS +D +GFL EL+ +GLD AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of CmaCh17G012480 vs. ExPASy Swiss-Prot
Match: O82357 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT1 PE=1 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 5.6e-152
Identity = 266/324 (82.10%), Postives = 300/324 (92.59%), Query Frame = 0 

Query: 22  TLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSGVHLDMAGI 81
           ++R+MSN+PENTVYGGP    PNQRVTLT+L+QK+++GEPIT+VTAYDYPS VHLD AGI
Sbjct: 17  SVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGI 76

Query: 82  DIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGARRPLLIGDLPFGTYESSSVQAV 141
           D+ LVGDSASMVVHGHDTTLPI+LDEMLVHCRAVARGA+RPLL+GDLPFGTYESSS QAV
Sbjct: 77  DVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAV 136

Query: 142 DTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 201
           DTAVRVLKEGGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVLGGFRPQG+
Sbjct: 137 DTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGR 196

Query: 202 NIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPFCSGQVLVY 261
           NIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPTIGIGAGPFCSGQVLVY
Sbjct: 197 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVY 256

Query: 262 HDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHSPYKISDND 321
           HDLLGM+QHPHHAKVTPKFCKQ+A +G+VINKAL +YKEEVS   FPGP HSPYKI+ ++
Sbjct: 257 HDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASE 316

Query: 322 YNGFLKELRSMGLDGAASAAAAKA 346
            +GFL EL+ +G D AASAAA  A
Sbjct: 317 LDGFLTELQKLGFDKAASAAALAA 340

[Close]
BLAST of CmaCh17G012480 vs. ExPASy Swiss-Prot
Match: Q9AWZ8 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT1 PE=2 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 3.0e-137
Identity = 249/334 (74.55%), Postives = 283/334 (84.73%), Query Frame = 0 

Query: 24  RYMSNVPENTVYGGPTSNT------------PNQRVTLTNLKQKYKKGEPITMVTAYDYP 83
           R +S+VPE+ VYGGP                  +RVT+T L+ K+++GEPIT+VTAYDYP
Sbjct: 18  RPLSHVPESAVYGGPRPQDVGAAAGAGAGAGATRRVTVTTLRGKHRRGEPITVVTAYDYP 77

Query: 84  SGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGARRPLLIGDLPFG 143
           S VH+D AGID+ LVGDSA+MVVHGHDTTLPI+LD ML HCRAVARGA RPLL+GDLPFG
Sbjct: 78  SAVHVDSAGIDVCLVGDSAAMVVHGHDTTLPISLDVMLEHCRAVARGATRPLLVGDLPFG 137

Query: 144 TYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAIS 203
            YESSS +AVD+AVRVLKEGGMDAIKLEGGA SRI+AA+AIVEAGIAVMGHVGLTPQAIS
Sbjct: 138 CYESSSTRAVDSAVRVLKEGGMDAIKLEGGAPSRISAAKAIVEAGIAVMGHVGLTPQAIS 197

Query: 204 VLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAG 263
           VLGGFRPQGK + SAVKVVETA++LQEAGCF+VVLECVP  VAAAAT+AL+IPTIGIGAG
Sbjct: 198 VLGGFRPQGKTVDSAVKVVETALALQEAGCFSVVLECVPAPVAAAATSALQIPTIGIGAG 257

Query: 264 PFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPL 323
           PFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQF  +G VINKALS+YK+EV   SFPGP 
Sbjct: 258 PFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQFGNVGHVINKALSEYKQEVETRSFPGPS 317

Query: 324 HSPYKISDNDYNGFLKELRSMGLDGAASAAAAKA 346
           H+PYKI+  D +GF   L+ MGLD AA+AAAA A
Sbjct: 318 HTPYKIAAADVDGFANALQKMGLDEAANAAAAAA 351

[Close]
BLAST of CmaCh17G012480 vs. ExPASy Swiss-Prot
Match: Q9AWZ7 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT2 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.5e-120
Identity = 223/326 (68.40%), Postives = 263/326 (80.67%), Query Frame = 0 

Query: 24  RYMSN-VPENTVYGG------PTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSGVHL 83
           R MSN   E  +YGG        S+   +RVTL  L+ K+++GEPI+MVTAYDYPSGVH+
Sbjct: 54  RAMSNGAAEPAIYGGGGGAQQAASSAAARRVTLATLRGKHRRGEPISMVTAYDYPSGVHV 113

Query: 84  DMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGARRPLLIGDLPFGTYESS 143
           D AG DI LVGDSA+MV HGHD TLPI+LD M+ HCRAVARGA R  L+GDLPFG+YE+S
Sbjct: 114 DAAGFDICLVGDSAAMVAHGHDNTLPISLDLMIEHCRAVARGAARTFLVGDLPFGSYEAS 173

Query: 144 SVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGF 203
           + QAV +AVRV+KEGG+++IKLEG A SRI+AARAIV+AGIAVMGH+GLTPQ++S LGGF
Sbjct: 174 TAQAVGSAVRVMKEGGVNSIKLEGSAPSRISAARAIVDAGIAVMGHIGLTPQSVSALGGF 233

Query: 204 RPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPFCSG 263
           RPQGK + SAVKVVE A++LQEAGCFAVVLECVP  VAAAAT+AL IPTIGIGAGPFCSG
Sbjct: 234 RPQGKTVESAVKVVEAALALQEAGCFAVVLECVPAPVAAAATSALTIPTIGIGAGPFCSG 293

Query: 264 QVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHSPYK 323
           QVLVYHDLLG  Q   HAKV+PKFCKQ+  IGDVIN+ALS YK+EV   SFPGP H+PYK
Sbjct: 294 QVLVYHDLLGTFQ-TSHAKVSPKFCKQYGNIGDVINRALSKYKQEVETQSFPGPSHTPYK 353

Query: 324 ISDNDYNGFLKELRSMGLDGAASAAA 343
           ++  D + FL  L+  GL+ AA AAA
Sbjct: 354 LAATDVDAFLNALKMKGLNVAADAAA 378

[Close]
BLAST of CmaCh17G012480 vs. ExPASy Swiss-Prot
Match: B0TC10 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) OX=498761 GN=panB PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.3e-72
Identity = 138/269 (51.30%), Postives = 191/269 (71.00%), Query Frame = 0 

Query: 45  QRVTLTNLKQKYKKGEPITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPIT 104
           +++TL   K+  K+G+ + M+TAYDYP    +D + I+I LVGDS  MVV G+D+T+P+T
Sbjct: 3   KKITLPQCKEMKKQGKRLRMITAYDYPFARLVDESEIEIILVGDSLGMVVLGYDSTVPVT 62

Query: 105 LDEMLVHCRAVARGARRPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHS 164
           LDEM+ HC+ V RGA   L++ D+PFG+Y  S   A+  A R+LKE G++A+K+EGG   
Sbjct: 63  LDEMIHHCKPVVRGAPNTLVVADMPFGSYNVSKEDAIRNANRMLKESGIEAVKVEGGTRM 122

Query: 165 RITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAV 224
             T  RA+V+AGI VMGH+GLTPQ  + LGGF+ QGK   +A +++E A++L+ AG F++
Sbjct: 123 APT-VRALVDAGIPVMGHIGLTPQTAAQLGGFKVQGKTEDAAQQLLEDALALESAGAFSI 182

Query: 225 VLECVPPEVAAAATAALRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQF 284
           V+ECVP  +A   TA+L IPTIGIGAGP+C+GQVLV  DLLG+     + +  PKF KQ+
Sbjct: 183 VIECVPAGLARTITASLSIPTIGIGAGPYCNGQVLVIQDLLGI-----YDRFVPKFVKQY 242

Query: 285 AQIGDVINKALSDYKEEVSNGSFPGPLHS 314
           AQ G  I  AL+DY  EV++G FPGP HS
Sbjct: 243 AQTGPAIRAALNDYAREVADGVFPGPEHS 265

[Close]
BLAST of CmaCh17G012480 vs. ExPASy TrEMBL
Match: A0A6J1JXR6 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488459 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 4.8e-191
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

[Close]
BLAST of CmaCh17G012480 vs. ExPASy TrEMBL
Match: A0A6J1GNW3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456114 PE=3 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.3e-188
Identity = 343/347 (98.85%), Postives = 346/347 (99.71%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALFNLVTKAAVRSTSQSLLNTLR MSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKISD+DYNGFLKELRSMGLDGAASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 347

[Close]
BLAST of CmaCh17G012480 vs. ExPASy TrEMBL
Match: A0A0A0K8B2 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G338120 PE=3 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.0e-177
Identity = 319/347 (91.93%), Postives = 335/347 (96.54%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALF L+T A  R+ +QSLL TLRYMSNVPENTVYGGPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPITLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAVR+LKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKISD D+NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of CmaCh17G012480 vs. ExPASy TrEMBL
Match: A0A1S3BH28 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103489751 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 318/347 (91.64%), Postives = 331/347 (95.39%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALF L+T AA R+  QSLL TLRYMSNVPENTVY GPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QAVDTAVR+LKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           EVSN SFPGP HSPYKISD D NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of CmaCh17G012480 vs. ExPASy TrEMBL
Match: A0A6J1D0E3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111016428 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.2e-170
Identity = 306/343 (89.21%), Postives = 326/343 (95.04%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           M LFN  T A  R+ ++SLL TLRYMSNVPENTVYGGP    P QRVTLTNL+QK+KKGE
Sbjct: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGIDIALVGDSASMVVHGHDTTLPI+LDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LKEGGMDAIKLEGGA SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAI+LQEAGCFAVVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVIN+AL DYKE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAA 344
           EV+NGSFPGPLHSPYKIS++DYNGFLKEL+++GLD AASAAAA
Sbjct: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 343

[Close]
BLAST of CmaCh17G012480 vs. NCBI nr
Match: XP_022991968.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 676.8 bits (1745), Expect = 1.0e-190
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

[Close]
BLAST of CmaCh17G012480 vs. NCBI nr
Match: KAG6575974.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 668.7 bits (1724), Expect = 2.7e-188
Identity = 343/347 (98.85%), Postives = 346/347 (99.71%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALFNLVTKAAVRSTSQSLLNTLR MSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE
Sbjct: 117 MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 176

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCR+VARGAR
Sbjct: 177 PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 236

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 237 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 296

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 297 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 356

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE
Sbjct: 357 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 416

Query: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKISD+DYNGFLKELRSMGLDGAASAAAAKAN+
Sbjct: 417 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 463

[Close]
BLAST of CmaCh17G012480 vs. NCBI nr
Match: XP_022953648.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita moschata] >XP_023547697.1 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 668.7 bits (1724), Expect = 2.7e-188
Identity = 343/347 (98.85%), Postives = 346/347 (99.71%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALFNLVTKAAVRSTSQSLLNTLR MSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKISD+DYNGFLKELRSMGLDGAASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 347

[Close]
BLAST of CmaCh17G012480 vs. NCBI nr
Match: KAG7014498.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 646.0 bits (1665), Expect = 1.9e-181
Identity = 343/395 (86.84%), Postives = 346/395 (87.59%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALFNLVTKAAVRSTSQSLLNTLR MSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE
Sbjct: 45  MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 104

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCR+VARGAR
Sbjct: 105 PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 164

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM
Sbjct: 165 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 224

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 225 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 284

Query: 241 LRIPTIGIGAGPFCSGQ------------------------------------------- 300
           LRIPTIGIGAGPFCSGQ                                           
Sbjct: 285 LRIPTIGIGAGPFCSGQTSFYMPYAHTHVHTYKAANRLGVSFSSSQLEKHRRSSLFSFPI 344

Query: 301 -----VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLH 348
                VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLH
Sbjct: 345 WSCSMVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLH 404

[Close]
BLAST of CmaCh17G012480 vs. NCBI nr
Match: XP_004139651.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KGN44587.1 hypothetical protein Csa_016545 [Cucumis sativus])

HSP 1 Score: 630.9 bits (1626), Expect = 6.3e-177
Identity = 319/347 (91.93%), Postives = 335/347 (96.54%), Query Frame = 0 

Query: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60
           MALF L+T A  R+ +QSLL TLRYMSNVPENTVYGGPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPITLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAVR+LKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           EVSNGSFPGPLHSPYKISD D+NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]
BLAST of CmaCh17G012480 vs. TAIR 10
Match: AT3G61530.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.0e-153
Identity = 269/334 (80.54%), Postives = 303/334 (90.72%), Query Frame = 0 

Query: 14  STSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSG 73
           S +   + T+R+MSNVPE+TVYGGP     NQRVTLT L+QK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPI+L+EMLVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVRVLKEGGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPF 253
           GGFRPQGKNIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPTIGIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKAL +YKEEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           PYKIS +D +GFL EL+ +GLD AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of CmaCh17G012480 vs. TAIR 10
Match: AT3G61530.2 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.0e-153
Identity = 269/334 (80.54%), Postives = 303/334 (90.72%), Query Frame = 0 

Query: 14  STSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSG 73
           S +   + T+R+MSNVPE+TVYGGP     NQRVTLT L+QK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPI+L+EMLVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVRVLKEGGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPF 253
           GGFRPQGKNIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPTIGIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKAL +YKEEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 348
           PYKIS +D +GFL EL+ +GLD AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]
BLAST of CmaCh17G012480 vs. TAIR 10
Match: AT2G46110.1 (ketopantoate hydroxymethyltransferase 1 )

HSP 1 Score: 538.5 bits (1386), Expect = 4.0e-153
Identity = 266/324 (82.10%), Postives = 300/324 (92.59%), Query Frame = 0 

Query: 22  TLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGEPITMVTAYDYPSGVHLDMAGI 81
           ++R+MSN+PENTVYGGP    PNQRVTLT+L+QK+++GEPIT+VTAYDYPS VHLD AGI
Sbjct: 17  SVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGI 76

Query: 82  DIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGARRPLLIGDLPFGTYESSSVQAV 141
           D+ LVGDSASMVVHGHDTTLPI+LDEMLVHCRAVARGA+RPLL+GDLPFGTYESSS QAV
Sbjct: 77  DVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAV 136

Query: 142 DTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 201
           DTAVRVLKEGGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVLGGFRPQG+
Sbjct: 137 DTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGR 196

Query: 202 NIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGIGAGPFCSGQVLVY 261
           NIASAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPTIGIGAGPFCSGQVLVY
Sbjct: 197 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVY 256

Query: 262 HDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKEEVSNGSFPGPLHSPYKISDND 321
           HDLLGM+QHPHHAKVTPKFCKQ+A +G+VINKAL +YKEEVS   FPGP HSPYKI+ ++
Sbjct: 257 HDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASE 316

Query: 322 YNGFLKELRSMGLDGAASAAAAKA 346
            +GFL EL+ +G D AASAAA  A
Sbjct: 317 LDGFLTELQKLGFDKAASAAALAA 340

The following BLAST results are available for this feature:
BLAST of CmaCh17G012480 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M3151.5e-15280.543-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis... [more]
O823575.6e-15282.103-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis... [more]
Q9AWZ83.0e-13774.553-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativ... [more]
Q9AWZ72.5e-12068.403-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativ... [more]
B0TC103.3e-7251.303-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum... [more]
BLAST of CmaCh17G012480 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JXR64.8e-191100.003-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1GNW31.3e-18898.853-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 G... [more]
A0A0A0K8B23.0e-17791.933-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3BH281.1e-17491.643-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1D0E36.2e-17089.213-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 ... [more]
BLAST of CmaCh17G012480 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022991968.11.0e-190100.003-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbit... [more]
KAG6575974.12.7e-18898.853-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucu... [more]
XP_022953648.12.7e-18898.853-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbit... [more]
KAG7014498.11.9e-18186.843-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucu... [more]
XP_004139651.16.3e-17791.933-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativ... [more]
BLAST of CmaCh17G012480 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G61530.11.0e-15380.54Phosphoenolpyruvate carboxylase family protein [more]
AT3G61530.21.0e-15380.54Phosphoenolpyruvate carboxylase family protein [more]
AT2G46110.14.0e-15382.10ketopantoate hydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003700Ketopantoate hydroxymethyltransferaseTIGRFAMTIGR00222TIGR00222coord: 48..313
e-value: 4.0E-88
score: 293.3
IPR003700Ketopantoate hydroxymethyltransferasePFAMPF02548Pantoate_transfcoord: 46..309
e-value: 2.7E-107
score: 358.0
IPR003700Ketopantoate hydroxymethyltransferasePANTHERPTHR208813-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 10..346
IPR003700Ketopantoate hydroxymethyltransferaseHAMAPMF_00156PanBcoord: 47..314
score: 40.042404
IPR003700Ketopantoate hydroxymethyltransferaseCDDcd06557KPHMT-likecoord: 49..308
e-value: 4.78715E-146
score: 410.657
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 44..326
e-value: 1.2E-120
score: 403.9
NoneNo IPR availablePIRSRPIRSR000388-1PIRSR000388-1coord: 55..309
e-value: 2.9E-89
score: 296.5
NoneNo IPR availablePANTHERPTHR20881:SF13-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 10..346
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 46..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G012480.1CmaCh17G012480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0015940 pantothenate biosynthetic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003864 3-methyl-2-oxobutanoate hydroxymethyltransferase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003824 catalytic activity