CmaCh17G004320 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh17G004320
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	gibberellin 20-oxidase-like protein
Location	Cma_Chr17: 2546838 .. 2547967 (-)
RNA-Seq Expression	CmaCh17G004320
Synteny	CmaCh17G004320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTGTCTCCTTCTTCCCTTTCTTTGCTAGCTGATGCTTGCAGAAAATGGGGCTTTTTCCATATCAAAAACCATGGAATCTCCAAAGATATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCTCTCTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCGTTCTTTGAAACTCTCAGAGTCTCTGGTCCTAACTTCTTAGCATCTGCTCAGAGTTCAGCAGATTTCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGGTACATACAATTCACAGCACATCTACTTCAAATCAGTATTGTTCTATTGTTCTAACAGTCTAATTTCATGTGCATAAAGTGAGATATTGCAAGACTATGGAGACAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATATTGATGAGCTTAGGAGAAGGTTTTGATACCACATACCATGAATCTCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAATTACACACCTCCAGAAACCTTGGGAGAAGATGAAGAACCAGAAGGACTTGGAATGCACACGGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGAGGTCTGCAAGTGAAATCAAGCGCAGGGGATTGGGTCGATATAGCCCCATGTGATGAATCCGAATCCGAAGCTCTTGTGGTGAATATTGGCGATCTTCTTCAAGCTTGGAGCAACGACAAGCTTAGGTCGTCTGAGCACAGAGTGGTTTTGCGGCGGCCGGCGATCAATCGGTTCTCGATTGCATTCTTCTGGGGGTTTGAGGACGAGAAGGTCGTGTCGGCGCCGGAGGAAGTGGTCGGCGACGGCGGTAAGAAACTGTATAAGCCATTTGTTTGCTCGGATTACCTGAAGTTCAGGGAGAATGACGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACTGTGAGAGATTTTGCTGGGAATGCGATTACGAAGGATTTTGAAGAAAAATAAATTTTTATTTTGTATTGCAATTTTATTCCAATTTATTAAATAAAAATAAGGG

mRNA sequence

ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTGTCTCCTTCTTCCCTTTCTTTGCTAGCTGATGCTTGCAGAAAATGGGGCTTTTTCCATATCAAAAACCATGGAATCTCCAAAGATATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCTCTCTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCGTTCTTTGAAACTCTCAGAGTCTCTGGTCCTAACTTCTTAGCATCTGCTCAGAGTTCAGCAGATTTCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGTGAGATATTGCAAGACTATGGAGACAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATATTGATGAGCTTAGGAGAAGGTTTTGATACCACATACCATGAATCTCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAATTACACACCTCCAGAAACCTTGGGAGAAGATGAAGAACCAGAAGGACTTGGAATGCACACGGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGAGGTCTGCAAGTGAAATCAAGCGCAGGGGATTGGGTCGATATAGCCCCATGTGATGAATCCGAATCCGAAGCTCTTGTGGTGAATATTGGCGATCTTCTTCAAGCTTGGAGCAACGACAAGCTTAGGTCGTCTGAGCACAGAGTGGTTTTGCGGCGGCCGGCGATCAATCGGTTCTCGATTGCATTCTTCTGGGGGTTTGAGGACGAGAAGGTCGTGTCGGCGCCGGAGGAAGTGGTCGGCGACGGCGGTAAGAAACTGTATAAGCCATTTGTTTGCTCGGATTACCTGAAGTTCAGGGAGAATGACGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACTGTGAGAGATTTTGCTGGGAATGCGATTACGAAGGATTTTGAAGAAAAATAAATTTTTATTTTGTATTGCAATTTTATTCCAATTTATTAAATAAAAATAAGGG

Coding sequence (CDS)

ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTGTCTCCTTCTTCCCTTTCTTTGCTAGCTGATGCTTGCAGAAAATGGGGCTTTTTCCATATCAAAAACCATGGAATCTCCAAAGATATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCTCTCTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCGTTCTTTGAAACTCTCAGAGTCTCTGGTCCTAACTTCTTAGCATCTGCTCAGAGTTCAGCAGATTTCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGTGAGATATTGCAAGACTATGGAGACAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATATTGATGAGCTTAGGAGAAGGTTTTGATACCACATACCATGAATCTCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAATTACACACCTCCAGAAACCTTGGGAGAAGATGAAGAACCAGAAGGACTTGGAATGCACACGGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGAGGTCTGCAAGTGAAATCAAGCGCAGGGGATTGGGTCGATATAGCCCCATGTGATGAATCCGAATCCGAAGCTCTTGTGGTGAATATTGGCGATCTTCTTCAAGCTTGGAGCAACGACAAGCTTAGGTCGTCTGAGCACAGAGTGGTTTTGCGGCGGCCGGCGATCAATCGGTTCTCGATTGCATTCTTCTGGGGGTTTGAGGACGAGAAGGTCGTGTCGGCGCCGGAGGAAGTGGTCGGCGACGGCGGTAAGAAACTGTATAAGCCATTTGTTTGCTCGGATTACCTGAAGTTCAGGGAGAATGACGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACTGTGAGAGATTTTGCTGGGAATGCGATTACGAAGGATTTTGAAGAAAAATAA

Protein sequence

MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSEILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK

Homology

[Close]

BLAST of CmaCh17G004320 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.6e-98
Identity = 183/326 (56.13%), Postives = 235/326 (72.09%), Query Frame = 0


Query: 7   SVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSET 66
           S+ELP+ DIS+PLS SSL+ L DAC++WGFF++ NHG+S+D+Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSEILQDY 126
           K+K+G     + YTP FIASPFFE+LRVSGP+F ASA+SS D   +Q +  +FS ++++Y
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDEEP---- 186
           G+KMT+L  KI+K IL S G+     Y+ES F NCHGY RINNYT P    +D       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 ---EGLGMHTDMSCVTIVYQDQTGGLQVKSSAG-DWVDIAPCDESESEALVVNIGDLLQA 246
              EGLGMHTDMSC+TIV QD  GGLQV++  G   +DI P D    EALVVN+GDLL A
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKD----EALVVNVGDLLHA 241

Query: 247 WSNDKLRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKVVSAPEEVVGD-GGKKLYKPFVC 306
           W+N +LRSS+HRV+L+R     NRFS+AFFW F+D KVV AP+EVVG   G ++++ F C
Sbjct: 242 WTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRVFRSFKC 301

Query: 307 SDYLKFRENDERGKFEKVGFTVRDFA 322
            DYL+FRE++E+GKFEKVG TV DFA
Sbjct: 302 GDYLRFRESNEKGKFEKVGDTVEDFA 319

[Close]

BLAST of CmaCh17G004320 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.5e-32
Identity = 101/311 (32.48%), Postives = 153/311 (49.20%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           +S       +P++D+S   +P   +L+ DA + WG F I NHGIS+ + + + S S  +F
Sbjct: 40  VSFDAVEESIPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLF 99

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPH--- 120
            +PSE KL+        +       SPFFE  ++    F  +  S  +  FN   PH   
Sbjct: 100 DMPSERKLEAASSDKGVSGYGEPRISPFFEK-KMWSEGFTIADDSYRNH-FNTLWPHDHT 159

Query: 121 QFSEILQDYGDKMTELSRKIIKNILMSLG---EGFDTTY--HESHFKNCHGYLRINNYTP 180
           ++  I+Q+Y D+M +L+ +++  IL SLG   E  +  +   +S  K   G +R+N+Y  
Sbjct: 160 KYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY-- 219

Query: 181 PETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNI 240
                E E   GL  HTD + +TI++Q  TGGLQV      WV + P        LVVNI
Sbjct: 220 -PVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA----PGVLVVNI 279

Query: 241 GDLLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP-EEVVGDGGKKLYK 300
           GDL    SN K+ S  HR  +     +R SIA+ WG     V  AP  ++ G     LY+
Sbjct: 280 GDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYR 337

Query: 301 PFVCSDYLKFR 303
                +YL+ +
Sbjct: 340 SITWKEYLQIK 337

[Close]

BLAST of CmaCh17G004320 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 8.0e-31
Identity = 101/304 (33.22%), Postives = 152/304 (50.00%), Query Frame = 0


Query: 10  LPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKLK 69
           +P++DIS     S    + DA  +WGFF + NHG+S ++   + + ++  F LP E K K
Sbjct: 62  IPVIDISNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRK 121

Query: 70  IGPLSSLNT-------YTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSEI 129
                SL+T       ++PH  A    E        F++ A++S      Q  P      
Sbjct: 122 FSREKSLSTNVRFGTSFSPH--AEKALEWKDYLSLFFVSEAEAS------QLWPDSCRSE 181

Query: 130 LQDYGDKMTELSRKIIKNILMSLGEGFDT----TYHESHFKNCHGYLRIN-NYTPPETLG 189
             +Y   M E ++ ++K +L  LGE  +        ES F    G  RIN NY P     
Sbjct: 182 TLEY---MNE-TKPLVKKLLRFLGENLNVKELDKTKESFFM---GSTRINLNYYP--ICP 241

Query: 190 EDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKS-SAGDWVDIAPCDESESEALVVNIGDLL 249
             E   G+G H+D+S +TI+ QD+ GGL V+S + G WV + P     S +LV+NIGD +
Sbjct: 242 NPELTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI----SGSLVINIGDAM 301

Query: 250 QAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCS 301
           Q  SN + +S EHR VL   + NR S+  F   + E V+    EV+ +G K +YK  + +
Sbjct: 302 QIMSNGRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKDILYT 343

[Close]

BLAST of CmaCh17G004320 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.0e-30
Identity = 92/310 (29.68%), Postives = 160/310 (51.61%), Query Frame = 0


Query: 3   VSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSL 62
           V++    +P++D+S P        + DA  KWGFF + NHG+  ++ + + + +++ F+L
Sbjct: 55  VNETDEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNL 114

Query: 63  PSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD-------FLFNQKS 122
           P E K K    +SL+T            T+R  G +F   A+ + +       F  ++  
Sbjct: 115 PVEEKRKFTKENSLST------------TVRF-GTSFSPLAEQALEWKDYLSLFFVSEAE 174

Query: 123 PHQF-SEILQDYGDKMTELSRKIIKNILMSLGEGFDT-TYHESHFKNCHGYLRIN-NYTP 182
             QF  +I ++   +    S+K+++ +L  LG+  +     E+      G +R+N NY P
Sbjct: 175 AEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYP 234

Query: 183 PETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKS-SAGDWVDIAPCDESESEALVVN 242
                  +   G+G H+D+S +TI+ QDQ GGL V+S ++G+WV + P     + + V+N
Sbjct: 235 --ICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPV----AGSFVIN 294

Query: 243 IGDLLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYK 302
           IGD +Q  SN   +S EHR VL     NR S+  F   + E V+    EV+ +G + +Y+
Sbjct: 295 IGDAMQIMSNGLYKSVEHR-VLANGYNNRISVPIFVNPKPESVIGPLPEVIANGEEPIYR 344

[Close]

BLAST of CmaCh17G004320 vs. ExPASy Swiss-Prot
Match: G9M9M2 (Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 4.5e-26
Identity = 89/302 (29.47%), Postives = 148/302 (49.01%), Query Frame = 0


Query: 10  LPILDISQ-PLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKL 69
           +P++D+S+    P    L+ +A  KWGFF I NHG+  ++  ++ + +   F LP+E K 
Sbjct: 57  IPVIDLSEWGSDPKVGDLICEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKN 116

Query: 70  KIGPLSSLN-------TYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSE 129
           K    +S +       ++TPH           +   +FL     S  ++ ++++   +  
Sbjct: 117 KHSKDNSPSNNVRYGTSFTPH-------AEKALEWKDFL-----SLFYVSDEEAAALWPS 176

Query: 130 ILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKN-CHGYLRIN-NYTPPETLGED 189
             +D           +IK +L SL +G + T  +   ++   G  RIN NY P       
Sbjct: 177 ACRDEALTFMRNCDAVIKRLLKSLMKGLNVTEIDGTKESLLMGSKRINMNYYP--KCPNP 236

Query: 190 EEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGD-WVDIAPCDESESEALVVNIGDLLQA 249
           E   G+G H+D+S +TI+ QDQ GGL V+    D WV + P     + A+V+N+GD LQ 
Sbjct: 237 ELTVGVGRHSDVSTLTILLQDQIGGLYVRKLDSDTWVHVPPI----NGAIVINVGDALQI 296

Query: 250 WSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDY 301
            SN + +S EHRV+    + NR S+  F       ++    E++  G K +YK  + SDY
Sbjct: 297 LSNGRYKSIEHRVI-ANGSNNRISVPIFVNPRPNDIIGPLPELLESGEKAVYKNVLYSDY 339

[Close]

BLAST of CmaCh17G004320 vs. ExPASy TrEMBL
Match: A0A6J1KY83 (gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 PE=3 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.5e-192
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS 240

Query: 241 NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK 300
           NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK
Sbjct: 241 NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK 300

Query: 301 FRENDERGKFEKVGFTVRDFAGNAITKDFEEK 333
           FRENDERGKFEKVGFTVRDFAGNAITKDFEEK
Sbjct: 301 FRENDERGKFEKVGFTVRDFAGNAITKDFEEK 332

[Close]

BLAST of CmaCh17G004320 vs. ExPASy TrEMBL
Match: A0A6J1H632 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111460359 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.4e-187
Identity = 327/338 (96.75%), Postives = 330/338 (97.63%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQ+YGDKMTELSRKIIKNILMSLGEGFDT YHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE------SEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE      SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFV 300
           LLQAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGK+LYKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 333
           CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 338

[Close]

BLAST of CmaCh17G004320 vs. ExPASy TrEMBL
Match: A0A5D3BVZ1 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001070 PE=3 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 3.8e-169
Identity = 292/327 (89.30%), Postives = 310/327 (94.80%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLP ETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQ+SA+FLFNQKS HQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQ+YG+KMTELSRKIIKN+L+S GEGF+  YHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+  ESEALVVNIGDLLQAWS
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE--ESEALVVNIGDLLQAWS 240

Query: 241 NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK 300
           ND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP+E+VG+GGK++YKPFVCSDYLK
Sbjct: 241 NDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFVCSDYLK 300

Query: 301 FRENDERGKFEKVGFTVRDFAGNAITK 328
           FRENDERGKFEKVGFTVRDFAG  I K
Sbjct: 301 FRENDERGKFEKVGFTVRDFAGKIINK 325

[Close]

BLAST of CmaCh17G004320 vs. ExPASy TrEMBL
Match: A0A1S3C8M6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LOC103497717 PE=3 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 3.8e-169
Identity = 292/327 (89.30%), Postives = 310/327 (94.80%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLP ETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQ+SA+FLFNQKS HQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQ+YG+KMTELSRKIIKN+L+S GEGF+  YHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+  ESEALVVNIGDLLQAWS
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE--ESEALVVNIGDLLQAWS 240

Query: 241 NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK 300
           ND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP+E+VG+GGK++YKPFVCSDYLK
Sbjct: 241 NDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFVCSDYLK 300

Query: 301 FRENDERGKFEKVGFTVRDFAGNAITK 328
           FRENDERGKFEKVGFTVRDFAG  I K
Sbjct: 301 FRENDERGKFEKVGFTVRDFAGKIINK 325

[Close]

BLAST of CmaCh17G004320 vs. ExPASy TrEMBL
Match: A0A0A0KB92 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G004480 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.2e-166
Identity = 287/322 (89.13%), Postives = 306/322 (95.03%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IYNKLHSFSNEIF
Sbjct: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           +LPSETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQ+SA+FLFNQKS HQFS
Sbjct: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQ+YGDKMTELSRKIIKNILMS GEGF+  YHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+  ESEALVVNIGDLLQAWS
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE--ESEALVVNIGDLLQAWS 240

Query: 241 NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK 300
           ND++RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE++V +GG+++YKPFVCSDYLK
Sbjct: 241 NDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLK 300

Query: 301 FRENDERGKFEKVGFTVRDFAG 323
           FRENDERGKFEKVGFTV +FAG
Sbjct: 301 FRENDERGKFEKVGFTVTNFAG 320

[Close]

BLAST of CmaCh17G004320 vs. NCBI nr
Match: XP_023006611.1 (gibberellin 20-oxidase-like protein [Cucurbita maxima])

HSP 1 Score: 681.0 bits (1756), Expect = 5.1e-192
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS 240

Query: 241 NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK 300
           NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK
Sbjct: 241 NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK 300

Query: 301 FRENDERGKFEKVGFTVRDFAGNAITKDFEEK 333
           FRENDERGKFEKVGFTVRDFAGNAITKDFEEK
Sbjct: 301 FRENDERGKFEKVGFTVRDFAGNAITKDFEEK 332

[Close]

BLAST of CmaCh17G004320 vs. NCBI nr
Match: XP_023547730.1 (gibberellin 20-oxidase-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 669.5 bits (1726), Expect = 1.5e-188
Identity = 329/336 (97.92%), Postives = 331/336 (98.51%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQ+YGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE----SEALVVNIGDLL 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE    SEALVVNIGDLL
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESDSEALVVNIGDLL 240

Query: 241 QAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCS 300
           QAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGK+LYKPFVCS
Sbjct: 241 QAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFVCS 300

Query: 301 DYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 333
           DYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK
Sbjct: 301 DYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 336

[Close]

BLAST of CmaCh17G004320 vs. NCBI nr
Match: XP_022959365.1 (gibberellin 20-oxidase-like protein [Cucurbita moschata])

HSP 1 Score: 665.2 bits (1715), Expect = 2.9e-187
Identity = 327/338 (96.75%), Postives = 330/338 (97.63%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQ+YGDKMTELSRKIIKNILMSLGEGFDT YHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE------SEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE      SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFV 300
           LLQAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGK+LYKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 333
           CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 338

[Close]

BLAST of CmaCh17G004320 vs. NCBI nr
Match: KAG7013737.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 663.7 bits (1711), Expect = 8.4e-187
Identity = 326/338 (96.45%), Postives = 330/338 (97.63%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQ+YGDKMTELSRKIIKNILMSLGEGFDT YHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE------SEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE      SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFV 300
           LLQAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGK+LYKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 333
           CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEE+
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEQ 338

[Close]

BLAST of CmaCh17G004320 vs. NCBI nr
Match: KAG6575175.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 661.8 bits (1706), Expect = 3.2e-186
Identity = 326/338 (96.45%), Postives = 329/338 (97.34%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQ+YGDKMTELSRKIIKNILMSLGEGFDT YHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE------SEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESE      SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFV 300
           LLQAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGK+LYKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 333
           CSDYLKFRENDERGKFEKVGFTVRDFAGNAITK FEEK
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKYFEEK 338

[Close]

BLAST of CmaCh17G004320 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 381.3 bits (978), Expect = 7.7e-106
Identity = 183/324 (56.48%), Postives = 250/324 (77.16%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MS   +S++LP+LD++QP+  S LS L++AC++WGFF++ NHGISK++++K+ S S ++F
Sbjct: 1   MSELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120
             P E+KLK+GP+S    YTP +IASP+FE+L VSGP+F  SA++SAD LF      +  
Sbjct: 61  KAPLESKLKLGPIS----YTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELR 120

Query: 121 EILQDYGDKMTELSRKIIKNIL-MSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGED 180
           E +Q+YG KM ELS+++IK +L M+LG+      +++ F NCHGYLR+ NYTPP  + + 
Sbjct: 121 ETMQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQ 180

Query: 181 EE-PEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQA 240
           EE  EGLGMHTDMSC+TIVYQD  GGLQ++S  G W+DI PC    ++ LVVNIGDL+QA
Sbjct: 181 EELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC----NDFLVVNIGDLMQA 240

Query: 241 WSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDY 300
           WSN +LRSSEHRVVLR+  +NR S+AFF  FEDEKV+ AP+E+VG+G ++ YK F CS+Y
Sbjct: 241 WSNGRLRSSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEY 300

Query: 301 LKFRENDERGKFEKVGFTVRDFAG 323
           LKFR+++E GKFEK+GFTV+DFAG
Sbjct: 301 LKFRQSNEEGKFEKIGFTVKDFAG 315

[Close]

BLAST of CmaCh17G004320 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.8e-99
Identity = 183/326 (56.13%), Postives = 235/326 (72.09%), Query Frame = 0


Query: 7   SVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSET 66
           S+ELP+ DIS+PLS SSL+ L DAC++WGFF++ NHG+S+D+Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSEILQDY 126
           K+K+G     + YTP FIASPFFE+LRVSGP+F ASA+SS D   +Q +  +FS ++++Y
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDEEP---- 186
           G+KMT+L  KI+K IL S G+     Y+ES F NCHGY RINNYT P    +D       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 ---EGLGMHTDMSCVTIVYQDQTGGLQVKSSAG-DWVDIAPCDESESEALVVNIGDLLQA 246
              EGLGMHTDMSC+TIV QD  GGLQV++  G   +DI P D    EALVVN+GDLL A
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKD----EALVVNVGDLLHA 241

Query: 247 WSNDKLRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKVVSAPEEVVGD-GGKKLYKPFVC 306
           W+N +LRSS+HRV+L+R     NRFS+AFFW F+D KVV AP+EVVG   G ++++ F C
Sbjct: 242 WTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRVFRSFKC 301

Query: 307 SDYLKFRENDERGKFEKVGFTVRDFA 322
            DYL+FRE++E+GKFEKVG TV DFA
Sbjct: 302 GDYLRFRESNEKGKFEKVGDTVEDFA 319

[Close]

BLAST of CmaCh17G004320 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 5.8e-69
Identity = 123/202 (60.89%), Postives = 160/202 (79.21%), Query Frame = 0


Query: 123 LQDYGDKMTELSRKIIKNIL-MSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDEE 182
           +Q+YG KM ELS+++IK +L M+LG+      +++ F NCHGYLR+ NYTPP  + + EE
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 183 -PEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNIGDLLQAWS 242
             EGLGMHTDMSC+TIVYQD  GGLQ++S  G W+DI PC    ++ LVVNIGDL+QAWS
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC----NDFLVVNIGDLMQAWS 120

Query: 243 NDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCSDYLK 302
           N +LRSSEHRVVLR+  +NR S+AFF  FEDEKV+ AP+E+VG+G ++ YK F CS+YLK
Sbjct: 121 NGRLRSSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLK 180

Query: 303 FRENDERGKFEKVGFTVRDFAG 323
           FR+++E GKFEK+GFTV+DFAG
Sbjct: 181 FRQSNEEGKFEKIGFTVKDFAG 197

[Close]

BLAST of CmaCh17G004320 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 141.7 bits (356), Expect = 1.0e-33
Identity = 101/311 (32.48%), Postives = 153/311 (49.20%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           +S       +P++D+S   +P   +L+ DA + WG F I NHGIS+ + + + S S  +F
Sbjct: 40  VSFDAVEESIPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLF 99

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPH--- 120
            +PSE KL+        +       SPFFE  ++    F  +  S  +  FN   PH   
Sbjct: 100 DMPSERKLEAASSDKGVSGYGEPRISPFFEK-KMWSEGFTIADDSYRNH-FNTLWPHDHT 159

Query: 121 QFSEILQDYGDKMTELSRKIIKNILMSLG---EGFDTTY--HESHFKNCHGYLRINNYTP 180
           ++  I+Q+Y D+M +L+ +++  IL SLG   E  +  +   +S  K   G +R+N+Y  
Sbjct: 160 KYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY-- 219

Query: 181 PETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESEALVVNI 240
                E E   GL  HTD + +TI++Q  TGGLQV      WV + P        LVVNI
Sbjct: 220 -PVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA----PGVLVVNI 279

Query: 241 GDLLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP-EEVVGDGGKKLYK 300
           GDL    SN K+ S  HR  +     +R SIA+ WG     V  AP  ++ G     LY+
Sbjct: 280 GDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYR 337

Query: 301 PFVCSDYLKFR 303
                +YL+ +
Sbjct: 340 SITWKEYLQIK 337

[Close]

BLAST of CmaCh17G004320 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 136.0 bits (341), Expect = 5.7e-32
Identity = 101/304 (33.22%), Postives = 152/304 (50.00%), Query Frame = 0


Query: 10  LPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKLK 69
           +P++DIS     S    + DA  +WGFF + NHG+S ++   + + ++  F LP E K K
Sbjct: 62  IPVIDISNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRK 121

Query: 70  IGPLSSLNT-------YTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFSEI 129
                SL+T       ++PH  A    E        F++ A++S      Q  P      
Sbjct: 122 FSREKSLSTNVRFGTSFSPH--AEKALEWKDYLSLFFVSEAEAS------QLWPDSCRSE 181

Query: 130 LQDYGDKMTELSRKIIKNILMSLGEGFDT----TYHESHFKNCHGYLRIN-NYTPPETLG 189
             +Y   M E ++ ++K +L  LGE  +        ES F    G  RIN NY P     
Sbjct: 182 TLEY---MNE-TKPLVKKLLRFLGENLNVKELDKTKESFFM---GSTRINLNYYP--ICP 241

Query: 190 EDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKS-SAGDWVDIAPCDESESEALVVNIGDLL 249
             E   G+G H+D+S +TI+ QD+ GGL V+S + G WV + P     S +LV+NIGD +
Sbjct: 242 NPELTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI----SGSLVINIGDAM 301

Query: 250 QAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFVCS 301
           Q  SN + +S EHR VL   + NR S+  F   + E V+    EV+ +G K +YK  + +
Sbjct: 302 QIMSNGRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKDILYT 343

The following BLAST results are available for this feature:

BLAST of CmaCh17G004320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	2.6e-98	56.13	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q9C971	1.5e-32	32.48	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9C899	8.0e-31	33.22	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S...	[more]
Q9LHN8	1.0e-30	29.68	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S...	[more]
G9M9M2	4.5e-26	29.47	Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 ...	[more]

BLAST of CmaCh17G004320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KY83	2.5e-192	100.00	gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 ...	[more]
A0A6J1H632	1.4e-187	96.75	gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11146035...	[more]
A0A5D3BVZ1	3.8e-169	89.30	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3C8M6	3.8e-169	89.30	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0KB92	5.2e-166	89.13	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]

BLAST of CmaCh17G004320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023006611.1	5.1e-192	100.00	gibberellin 20-oxidase-like protein [Cucurbita maxima]	[more]
XP_023547730.1	1.5e-188	97.92	gibberellin 20-oxidase-like protein [Cucurbita pepo subsp. pepo]	[more]
XP_022959365.1	2.9e-187	96.75	gibberellin 20-oxidase-like protein [Cucurbita moschata]	[more]
KAG7013737.1	8.4e-187	96.45	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. argy...	[more]
KAG6575175.1	3.2e-186	96.45	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro...	[more]

BLAST of CmaCh17G004320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	7.7e-106	56.48	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	1.8e-99	56.13	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	5.8e-69	60.89	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80330.1	1.0e-33	32.48	gibberellin 3-oxidase 4	[more]
AT1G55290.1	5.7e-32	33.22	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 10..72 e-value: 1.4E-14 score: 54.9
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..301 e-value: 1.0E-68 score: 234.3
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 166..266 e-value: 1.7E-19 score: 70.2
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..319
None	No IPR available	PANTHER	PTHR47990:SF52	GIBBERELLIN 3-BETA-DIOXYGENASE 4	coord: 4..319
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 7..305
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 161..269 score: 11.727542

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh17G004320.1	CmaCh17G004320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901576	organic substance biosynthetic process
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen