CmaCh17G002440 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G002440
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionvacuole membrane protein KMS1-like
LocationCma_Chr17: 1239972 .. 1245561 (+)
RNA-Seq ExpressionCmaCh17G002440
SyntenyCmaCh17G002440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACAAACATTTTCTCAAAATTGGGGGAAAATAAATATCGATCGAGGAAAGAACCAAAGATTCGATCTTGGAGGGGCGAATGAAGACGCGATAAACTCTGCAGAGAGGATTTTCGTTCCAAAAACGCCTTTGATTTCCCTCTCTTTCTCTCTCTCTATCCTCCCTTTGTTTTCCGATCTTCAAGAACAGGTGCTGGATTTTGAATTTTTGCTATCTGTTTTTTCCTCTCGATTCTCGTCATGATGTTTGTCGATCTCTGATCTAGATGTTACCATTTTTTTTTTTAATACAAGAGATTACGATAGCAAGTAGATTTTTTTTTCTCTCCGTTTTCTCCCTTTCGATACGGTCTTCCAATTGCTGTTCGCGGAATGGGGACTGTTGAAGAAGCAGCTTCCATTACTTCTCATGATTCTGAAGTATCCATTTCAGGCAAGTATTCCTGTCTCTCGTAATTTATCTTTTGTTCGGCCGAAGCATTGATACAAGAAAAGTTGAAGGAAATTAGGGCCTTCTAATTTGGAAGCTTGTTTTAATCATTTAGTATTTCATTGATAGATTTTGATGCTAAGACTCGTATCTGCAAGAAGGGTAGTTGATGTTGTAATTGGAGCCTTATGTTTATATATTGTTTATATTGTCATTATTGTTAACTTTTTAGAGAGAATTGAGGCTGGTGATTGGCTGAACTGTGTTAATGAAGTGAAATGCATTTGTTAATGCGTGGTAAGGGGCTTTGATTGATTGGCTTTACTGGTTAATTGCAAACAAGCGTGGAAACATGAATATTAGTTCCGGATCTATTGGAGTTTCAAAACTGAAATGCATTTGTGCCTTGCTTCTTCAGTTCAATTTGTGTCCACGGATTTACTGTTTTAGTTGTTGCTTTCACTTGAGTTTTTATGGAACTAACGAGAACGCATATTCTCTCGTATTCAACCTAACTTGGGATCGTTCACATTGGATTTTATTACCTAATGAAGTTACCATACTTTGCAGAGCTACGTAGAAAGCAGAAGGAAGAACTTGAAAATTTGACATTGGTGACTCAACCATTCAAAACATTGAAGTTTTTCATTTTGGGTGTTCTTCAATATAGCAAACACTCGATTGCATACATTTTAGCGAAAGGTGGTTGGTTTTTGCTCTTACACTTGATTATTGGGGTAGCTGGAATTTTGATTTTGACTATCGAAGGGCCACATGAGAAGGTATCTCTGCCTGTTTGTATTCATGTTAGCTTCAGATTTTCACTTATTTCAACACTGTGTGTGTATTTATGCATCTGCAAGTTTTACTTTATTTTGGATAAACCATGATAGTCTGTTCCTGCTGAAATGTGGAAGCATCTTTGTGATTCGATACGGTATATTAGTATGGCAGCCTTCTGGATGTATATTCTATATTTTATCGACATTTTTCTAGGTTTATGATATTGTTTTATGATATTGCCTTATGATATTGTTTTATCATGGATGCCTACGAGTGATGTTTTGTAACGGCCCAAGTCCACTGCTAGCAGATATTGCCCACAAGGTTTTTAAAACGCGTCTACTAGGGAGAGGTTTCCAACCCTTATAAAGAATGCTTTGTTCCCCTCTCCAACCGACGTCAGATCTTACATGTTCCATGAAGAAAAGTCGTGTATTTAAATTTCTGCCTATTTGTGTAGGCTCTTGAGAGATACTATATTTATAGCTTTGAAAATTCTGCCACAAAGTTTATACACCACATGTATAATCGATGAGGTTGGGGCTTGCACTATTTTGTGGGGGCTTTGGGGGAAACAACAAAATCTTTAGAAGATAGTGAGAGTTCTTATGGCTGTTTTTGGTCCTTTGTTAGATTCCCTGTTTCTCCTTGGGCCTTTGTTTCGAGGCTTGTTTGTAACTATTTAGTTTGGAGACGTCTCCTTTTTGTGGACTTCTCCTTCTTTTGTATGCCCATGTATTCTTTCATTTTATCTGAATGAAAGTCAAGTTTCTTCGTTAAAAAAAAAAGAAAAAAAAGAAAAATCAAATCCAGTATAAATGCCATATTACAGAAGAGATTGAAACTTGTAAGTATTTAGAGGAATTGTTAAGATGGTGGATAGAGAAGAGATGTAGGATGTCCTAATCAAACTGTTCTTCATAAACTACCTTGTTCCTCCTTTATATTCAGGAAGTCGATTTATTTCTGTTCTTGACATTATTGGCTTTTTCCTATTGATTCGTCCACTGCCTTCCAGTTTTGAGGCAATCATTTTATCAAATAAATCCTCTGTGTTTTGCAGCATGTTGAGGAGATTGTGAAATACTTTGACTACGGCTTGTGGTGGGTGATCCTTGGAGTTGCATCATCAATTGGTCTTGGTAATTAATGTTGTTTGGTTTAAATGATGGTTAGCAATGCTGATTATTGCTGCTTCTTTGAAAAGGCAACATTTTTCTATGATATTTAGATTGCATCTTTCTGTTATGATCAAGATTGTATTTAGTGTATTATTTCACTCCCTATCATTTACTTTCAGGGTCTGGTCTGCATACTTTTGTCCTTTATCTTGGGCCTCACATTGGGTTTTTCACAATAAAGGCGGTGCAGTGTGGTCGAGTGGACTTGAAGAGTGCCACTTATGACACAATACAATTGAAAAGAGGCCCATCGTGGCTCGAGAAGGATTGTTCAGAATTTGGACCTGCAGTTTTCACATCACGAGTCCCACTCAGCAGCATATTGCCAATGGTTCAGATAGAGGCAATTCTGTGGGGTATTGGGACCGCCCTAGGAGAGCTTCCTCCTTATTTTATCTCAAGGGCAGGTATGAGAAAATAAGTACCTATTGGTATTCCTAAAAGTTATAATGTCAATTCCTCCTTATTTTCTAGTGATGTTTACATGATTCTTGATAATGGAGGTGATATCATTCATTCCTCTTGAATGATAATAGATGGTATAGAAGCACGAGTATGAATAAATCTATTCGAACTTTGTGAACATGTTGATTACTTTAGTTGAATGTCTGTTGTGATCTGAAATGGTTCAAACTTCTTTGTGATCTTTTTCCCAATGGGAGAGACAAATTCTTGATATATCATCTAATATACGATGCATTAAAATTTTTTTAACATATCGATTACTATAGTTGAATGTTTACTGCGATCTTAAATATTTCAAACTTTTTTGTGATCTTTTACCAATTGGAGATGTAAAATTCTTTCTCACTACTACAGCCTTTTCGTGATATTAATAATTGGAAAGGTCTCCTCTAGTTTGCGCTGGGTGGCGAATCCCTCTTCTCCTATCTAATCAATGATATGCTATCATGGGAAGGAACTATTGGGCAAGACATTTGGCTTCAAGGTCATCGTGCAGTTAGTTTTAGCTTATGATTAATTGGTTTAGAGATCTCATATTGGTTAGAGAGGGGAATGAAACATTCTTTATAAGGGTGTGGAAACCTCTCTTTAGTAGATGCGTTTTAAAAACCTCGAGGGGAAGCCCGAAAGAGAAAATCCAAGGAGGACAATATCTATTCGCTGTGGGCTTGGATTGATACAAATGGTATCAAAGCCAGGCACTGAGTGGTGTGCCAGCGAGGACGCTAGACCCCTAAGGGGTGGATTGTAAGATCCCACATTGGTTGGAGAGGAAAGTGAAGTGTTCCTTATAAGTGTATGGAAACCTCTCCTTAGTAGACATGTTTTAAAACTATGAGGCTGACAGCGATACATAACGAGTCAAAGCGGACAATATCTGCTAGCGGTGGGGCTTGGATGGTTACAATTGGTATTGGAGCCAGACACGGGGCGATGTGCCAAGGAGGAGGCTGAACCCCGAAGGGGAGTGGACACTAGGCATTGTGCCAGCGAGGACTTCTTTTTTAAACCTTTCTTTTGTCAAGATGATGATAGAGAATTATGAAGGAGCAGAAACTGTATTGACAAACCGGAGCTCGCTCTAGTTTTTAATTTGAACTTTGCGATCAGTGCTATGTTTTAGATCAACAATACGAGTCTGTGAACTGAAATAGTCCAATCGCAAAATAACATGTTCTAGTATGTTTATTGTTTTGCTGCTGTACTGTTCTTGGCATTGCTGTTATGCTTTTCATATCTGTTCAAATATATTTCTTCCATTATGCAGCTCGCCTCTCCGGTGGCAGATCAGAAGCAATGGAAGAACTAGATGCCTCTTCGAGGGAAACTAATGGATTCATTCCAACATACTTAAATAGAGTGAAGCGATGGTTCCTTTCACACGCTCAACATTTGAATTTCCTTACTATTCTACTGCTTGCTTCGGTTAGTCAAGACTTTATTCTTTCATGTTTCTTTGCCACTGCTTGCTAGTTTCATGTGATTTTTCTCCCTTCATCTTAAGTTGTTCAAAGGAACACATTCACGCCTACCCGCCTCGGTTATTAAACATCTTATTCGATTTTCGAGAGTTGTCTAGTGGGATAAAATTTCACGTAAGCTAGTCTTAATGCCTGGATTTGTTCAGAGAAATGAAAGTACGTGCATTTCTGCTATCTTTCAGGTTCCAAATCCACTGTTTGACTTGGCGGGCATTATGTGTGGACAATTCGGGATTCCTTTCTGGGAATTCTTTTTCGCTACGCTGGTCGGAAAAGCAATTATCAAAACTCACATCCAGGTTTGTAAAGGTTCTATATCTTGACTTGGTTTGCAAGGAGCTAAGTTGTTATCAATCTATAAACTTCTGCACTTTCTGAACGCCACTCACTCCCTTCTACATCCTTTGATAGCTCGCTCTGTGTTTATCATGTCCTGTGCAGACCATTTTCATCATTGCCGTTTGCAACAATCAACTTCTTGATTGGATAGAAAACGAACTAATATGGATACTCAGCTTCGTGCCTGGATTTTCATCGGTCTTGCCCGAGCTCATTTCCAAGCTCAATGCAATCAAAGCTAAGTATCTGAAAGCGCCATCTCATGCGGCCACTAATGTCAAGGTAACTCTCTGAACATCCTATTGATCTGTTTGCCTGAGCTTGTCTCTGCTAAAATGGGCCATTGTCACATTCAGGCCAAATGGGATTTCTCAGTTTCATCCATCTGGAACACTATAGTATGGCTCATGATCATGAACTTCTTTGTCAAGATTATGACTTCAACTGCCCAAAGGTACTTGAAGCAACAGCAAGATGAGGAGCTGACTGCGTTGACGAACAAAGTTCCTGCATTGGCATGCTCGAATTAACCCGGTTCTAACGTCCTCTCTTTTATCACTCATATACTTTACTAAGATTTAGCATCAACTGTTTCGATTCGAGTAATCGAGGATATGTCAGGAGAGAAGAAACATTGTTTTAGAAAGCTGTTTAGAATAGGAAAGGTGCTATCCATGATATGTAGTGTCATAAACCCAGTTCTGATTGGGGGGAGGGAACAGTTACTTTGGGTAATTATATTAATTTACCCACTGTTAGTTAACCATTGGAAACATTGGAACATACATCATTATTCATTCTTAATCCCAGACATATTGTGATTCATTACTGCCTAAATCACTCATTTTTATTTTTGAAGCGTTCTAAAGCTACTTTTGGAGTAGAAGAACGATGAATGAAAAGTTTATGTAAT

mRNA sequence

GACAAACATTTTCTCAAAATTGGGGGAAAATAAATATCGATCGAGGAAAGAACCAAAGATTCGATCTTGGAGGGGCGAATGAAGACGCGATAAACTCTGCAGAGAGGATTTTCGTTCCAAAAACGCCTTTGATTTCCCTCTCTTTCTCTCTCTCTATCCTCCCTTTGTTTTCCGATCTTCAAGAACAGAGATTACGATAGCAAGTAGATTTTTTTTTCTCTCCGTTTTCTCCCTTTCGATACGGTCTTCCAATTGCTGTTCGCGGAATGGGGACTGTTGAAGAAGCAGCTTCCATTACTTCTCATGATTCTGAAGTATCCATTTCAGAGCTACGTAGAAAGCAGAAGGAAGAACTTGAAAATTTGACATTGGTGACTCAACCATTCAAAACATTGAAGTTTTTCATTTTGGGTGTTCTTCAATATAGCAAACACTCGATTGCATACATTTTAGCGAAAGGTGGTTGGTTTTTGCTCTTACACTTGATTATTGGGGTAGCTGGAATTTTGATTTTGACTATCGAAGGGCCACATGAGAAGCATGTTGAGGAGATTGTGAAATACTTTGACTACGGCTTGTGGTGGGTGATCCTTGGAGTTGCATCATCAATTGGTCTTGGGTCTGGTCTGCATACTTTTGTCCTTTATCTTGGGCCTCACATTGGGTTTTTCACAATAAAGGCGGTGCAGTGTGGTCGAGTGGACTTGAAGAGTGCCACTTATGACACAATACAATTGAAAAGAGGCCCATCGTGGCTCGAGAAGGATTGTTCAGAATTTGGACCTGCAGTTTTCACATCACGAGTCCCACTCAGCAGCATATTGCCAATGGTTCAGATAGAGGCAATTCTGTGGGGTATTGGGACCGCCCTAGGAGAGCTTCCTCCTTATTTTATCTCAAGGGCAGCTCGCCTCTCCGGTGGCAGATCAGAAGCAATGGAAGAACTAGATGCCTCTTCGAGGGAAACTAATGGATTCATTCCAACATACTTAAATAGAGTGAAGCGATGGTTCCTTTCACACGCTCAACATTTGAATTTCCTTACTATTCTACTGCTTGCTTCGGTTCCAAATCCACTGTTTGACTTGGCGGGCATTATGTGTGGACAATTCGGGATTCCTTTCTGGGAATTCTTTTTCGCTACGCTGGTCGGAAAAGCAATTATCAAAACTCACATCCAGACCATTTTCATCATTGCCGTTTGCAACAATCAACTTCTTGATTGGATAGAAAACGAACTAATATGGATACTCAGCTTCGTGCCTGGATTTTCATCGGTCTTGCCCGAGCTCATTTCCAAGCTCAATGCAATCAAAGCTAAGTATCTGAAAGCGCCATCTCATGCGGCCACTAATGTCAAGGCCAAATGGGATTTCTCAGTTTCATCCATCTGGAACACTATAGTATGGCTCATGATCATGAACTTCTTTGTCAAGATTATGACTTCAACTGCCCAAAGGTACTTGAAGCAACAGCAAGATGAGGAGCTGACTGCGTTGACGAACAAAGTTCCTGCATTGGCATGCTCGAATTAACCCGGTTCTAACGTCCTCTCTTTTATCACTCATATACTTTACTAAGATTTAGCATCAACTGTTTCGATTCGAGTAATCGAGGATATGTCAGGAGAGAAGAAACATTGTTTTAGAAAGCTGTTTAGAATAGGAAAGGTGCTATCCATGATATGTAGTGTCATAAACCCAGTTCTGATTGGGGGGAGGGAACAGTTACTTTGGGTAATTATATTAATTTACCCACTGTTAGTTAACCATTGGAAACATTGGAACATACATCATTATTCATTCTTAATCCCAGACATATTGTGATTCATTACTGCCTAAATCACTCATTTTTATTTTTGAAGCGTTCTAAAGCTACTTTTGGAGTAGAAGAACGATGAATGAAAAGTTTATGTAAT

Coding sequence (CDS)

ATGGGGACTGTTGAAGAAGCAGCTTCCATTACTTCTCATGATTCTGAAGTATCCATTTCAGAGCTACGTAGAAAGCAGAAGGAAGAACTTGAAAATTTGACATTGGTGACTCAACCATTCAAAACATTGAAGTTTTTCATTTTGGGTGTTCTTCAATATAGCAAACACTCGATTGCATACATTTTAGCGAAAGGTGGTTGGTTTTTGCTCTTACACTTGATTATTGGGGTAGCTGGAATTTTGATTTTGACTATCGAAGGGCCACATGAGAAGCATGTTGAGGAGATTGTGAAATACTTTGACTACGGCTTGTGGTGGGTGATCCTTGGAGTTGCATCATCAATTGGTCTTGGGTCTGGTCTGCATACTTTTGTCCTTTATCTTGGGCCTCACATTGGGTTTTTCACAATAAAGGCGGTGCAGTGTGGTCGAGTGGACTTGAAGAGTGCCACTTATGACACAATACAATTGAAAAGAGGCCCATCGTGGCTCGAGAAGGATTGTTCAGAATTTGGACCTGCAGTTTTCACATCACGAGTCCCACTCAGCAGCATATTGCCAATGGTTCAGATAGAGGCAATTCTGTGGGGTATTGGGACCGCCCTAGGAGAGCTTCCTCCTTATTTTATCTCAAGGGCAGCTCGCCTCTCCGGTGGCAGATCAGAAGCAATGGAAGAACTAGATGCCTCTTCGAGGGAAACTAATGGATTCATTCCAACATACTTAAATAGAGTGAAGCGATGGTTCCTTTCACACGCTCAACATTTGAATTTCCTTACTATTCTACTGCTTGCTTCGGTTCCAAATCCACTGTTTGACTTGGCGGGCATTATGTGTGGACAATTCGGGATTCCTTTCTGGGAATTCTTTTTCGCTACGCTGGTCGGAAAAGCAATTATCAAAACTCACATCCAGACCATTTTCATCATTGCCGTTTGCAACAATCAACTTCTTGATTGGATAGAAAACGAACTAATATGGATACTCAGCTTCGTGCCTGGATTTTCATCGGTCTTGCCCGAGCTCATTTCCAAGCTCAATGCAATCAAAGCTAAGTATCTGAAAGCGCCATCTCATGCGGCCACTAATGTCAAGGCCAAATGGGATTTCTCAGTTTCATCCATCTGGAACACTATAGTATGGCTCATGATCATGAACTTCTTTGTCAAGATTATGACTTCAACTGCCCAAAGGTACTTGAAGCAACAGCAAGATGAGGAGCTGACTGCGTTGACGAACAAAGTTCCTGCATTGGCATGCTCGAATTAA

Protein sequence

MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRVPLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALACSN
Homology
BLAST of CmaCh17G002440 vs. ExPASy Swiss-Prot
Match: Q5XF36 (Vacuole membrane protein KMS1 OS=Arabidopsis thaliana OX=3702 GN=KMS1 PE=1 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 1.7e-166
Identity = 283/412 (68.69%), Postives = 344/412 (83.50%), Query Frame = 0

Query: 7   AASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGG 66
           +A + S  S+V+IS LR K ++E+ENLTL TQP  TLK F+   +QY K SI+Y+LA GG
Sbjct: 3   SAGVASSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGG 62

Query: 67  WFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVL 126
           WF+L+  ++ V+G L++T++GPH KHVEE+++Y  YGLWW+ LGVASSIGLGSGLHTFVL
Sbjct: 63  WFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVL 122

Query: 127 YLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFT----SRVPL 186
           YLGPHI  FT+KA  CGRVDLKSA YDTIQLKR PSWL+K CSEFGP +      SRVPL
Sbjct: 123 YLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPL 182

Query: 187 SSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYL 246
           +SILP VQ+EAILWGIGTALGELPPYFISRAA +SG   + MEELD SS E +GF+ T+L
Sbjct: 183 TSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHL 242

Query: 247 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 306
           NRVKRW L+H+QHLNF T+L+LASVPNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKT
Sbjct: 243 NRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKT 302

Query: 307 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAAT 366
           HIQTIFII VCNNQLLDW+ENELIWILS VPG +S+LP L +KL+A+K KY+ APS   +
Sbjct: 303 HIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPS 362

Query: 367 NVKA-KWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTN 414
           ++K  KWDFS +SIWN IVWLM++NFFVKI+T+TAQR+LK++Q++E+  LT+
Sbjct: 363 HIKVKKWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATLTH 414

BLAST of CmaCh17G002440 vs. ExPASy Swiss-Prot
Match: F4I8Q7 (Vacuole membrane protein KMS2 OS=Arabidopsis thaliana OX=3702 GN=KMS2 PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 4.2e-154
Identity = 271/399 (67.92%), Postives = 322/399 (80.70%), Query Frame = 0

Query: 18  SISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGGWFLLLHLIIGV 77
           +IS LR K +++LE LTL +QPFKTL+ F++ V  Y +   +Y+LA  GW +L   I   
Sbjct: 13  AISGLREKHQQDLEKLTLTSQPFKTLRLFVVAVFLYVRRWSSYLLANVGWLILFCSIFVA 72

Query: 78  AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
              L++T++GPH KHVEE+ +Y  +GLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FTI
Sbjct: 73  FAALLVTLDGPHVKHVEELSEYTRFGLWWIFLGVASSIGLGSGLHTFVLYLGPHIALFTI 132

Query: 138 KAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRVPLSSILPMVQIEAILWG 197
           K +QCGRVDLKSA YDTIQLKR PSWL+K C EFG  VF+S VPLSSILP VQIEAILWG
Sbjct: 133 KVMQCGRVDLKSAIYDTIQLKRSPSWLDKPCHEFGSPVFSSGVPLSSILPQVQIEAILWG 192

Query: 198 IGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYLNRVKRWFLSHAQHLN 257
           +GTALGELPPYFISRAA LSGG+   M+EL+  S + NGFI   +N++K W LSH+Q+LN
Sbjct: 193 LGTALGELPPYFISRAASLSGGK---MKELETCSGDDNGFIAKRVNQIKSWLLSHSQYLN 252

Query: 258 FLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNNQL 317
           F TIL+LASVPNPLFDLAGIMCGQF  PFWEFF ATL+GKAIIKTHIQT+FII VCNNQL
Sbjct: 253 FFTILILASVPNPLFDLAGIMCGQFEKPFWEFFLATLIGKAIIKTHIQTVFIICVCNNQL 312

Query: 318 LDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKA--PSHAATNVKAKWDFSVSSI 377
           LDW+ENELI+ILSFVPGF+S LPEL +KL  +K KYL A  P  +  NVK KWD S +S+
Sbjct: 313 LDWVENELIYILSFVPGFASALPELTAKLRLMKEKYLIASPPVSSDINVK-KWDLSFASV 372

Query: 378 WNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNK 415
           WN +VWLM++NFF +I+TSTAQRYLK+QQ+EEL ALTNK
Sbjct: 373 WNGVVWLMLLNFFGQIVTSTAQRYLKKQQEEELDALTNK 407

BLAST of CmaCh17G002440 vs. ExPASy Swiss-Prot
Match: Q5R9K4 (Vacuole membrane protein 1 OS=Pongo abelii OX=9601 GN=VMP1 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 7.5e-58
Identity = 142/389 (36.50%), Postives = 212/389 (54.50%), Query Frame = 0

Query: 18  SISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGGWFLLLHLIIGV 77
           S++E +R+++EE +N+ L  QP  TL++F L +L   K   + +  +    +   L++ V
Sbjct: 29  SVNEKKRREREERQNIVLWRQPLITLQYFSLEILVILKEWTSKLWHRQSIVVSFLLLLAV 88

Query: 78  AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
             I    +EG H+++V+ I K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI   T+
Sbjct: 89  L-IATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTL 148

Query: 138 KAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRVPLSSILPMVQIEAILWG 197
            A +C  V+     Y            +  C + G    T  + L SI+  V+IEA +WG
Sbjct: 149 AAYECNSVNFPEPPYPD----------QIICPDEGGTEGT--ISLWSIISKVRIEACMWG 208

Query: 198 IGTALGELPPYFISRAARLSGGR--SEAMEELDASSRETNGFIPTYLNRVKRWFLSHAQH 257
           IGTA+GELPPYF++RAARLSG     E  +E +            + +R K       Q 
Sbjct: 209 IGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAES-AQDFASRAKLAVQKLVQK 268

Query: 258 LNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNN 317
           + F  IL  AS+PNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I   + 
Sbjct: 269 VGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSK 328

Query: 318 QLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAATNVKAKWDFSVSSI 377
            +++    +++  +  VPG    L +   +    + + L   S   T     W   +S +
Sbjct: 329 HIVE----QMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGTPQGENW---LSWM 388

Query: 378 WNTIVWLMIMNFFVKIMTSTAQRYLKQQQ 405
           +  +V +M+  F + I+ S AQ Y K+ Q
Sbjct: 389 FEKLVVVMVCYFILSIINSMAQSYAKRIQ 396

BLAST of CmaCh17G002440 vs. ExPASy Swiss-Prot
Match: Q99KU0 (Vacuole membrane protein 1 OS=Mus musculus OX=10090 GN=Vmp1 PE=1 SV=2)

HSP 1 Score: 224.2 bits (570), Expect = 2.8e-57
Identity = 142/389 (36.50%), Postives = 209/389 (53.73%), Query Frame = 0

Query: 18  SISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGGWFLLLHLIIGV 77
           S+ E +R+ +EE +N+ L  QP  TL++F L  L   K   + +  +    ++  L++  
Sbjct: 29  SVHEKKRRDREERQNIVLWRQPLITLQYFSLETLVVLKEWTSKLWHRQS-IVVSFLLLLA 88

Query: 78  AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
           A +    +EG H+++V+ I K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI   T+
Sbjct: 89  ALVATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTL 148

Query: 138 KAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRVPLSSILPMVQIEAILWG 197
            A +C  V+     Y            +  C E   A     + L SI+  V+IEA +WG
Sbjct: 149 AAYECNSVNFPEPPYPD----------QIICPEEEGA--EGAISLWSIISKVRIEACMWG 208

Query: 198 IGTALGELPPYFISRAARLSGGR--SEAMEELDASSRETNGFIPTYLNRVKRWFLSHAQH 257
           IGTA+GELPPYF++RAARLSG     E  +E +            + +R K       Q 
Sbjct: 209 IGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAEA-AQDFASRAKLAVQKLVQK 268

Query: 258 LNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNN 317
           + F  IL  AS+PNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I   + 
Sbjct: 269 VGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIVTFSK 328

Query: 318 QLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAATNVKAKWDFSVSSI 377
            +++    +++  +  VPG    L +   +    + + L   S A T     W   +S +
Sbjct: 329 HIVE----QMVTFIGAVPGIGPSLQKPFQEYLEAQRQKLHHRSEAGTPQGENW---LSWM 388

Query: 378 WNTIVWLMIMNFFVKIMTSTAQRYLKQQQ 405
           +  +V  M+  F + I+ S AQ Y K+ Q
Sbjct: 389 FEKLVVAMVCYFVLSIINSMAQNYAKRIQ 396

BLAST of CmaCh17G002440 vs. ExPASy Swiss-Prot
Match: Q96GC9 (Vacuole membrane protein 1 OS=Homo sapiens OX=9606 GN=VMP1 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 3.7e-57
Identity = 140/389 (35.99%), Postives = 209/389 (53.73%), Query Frame = 0

Query: 18  SISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGGWFLLLHLIIGV 77
           S++E +R+++EE +N+ L  QP  TL++F L +L   K   + +  +    +   L++ V
Sbjct: 29  SVNEKKRREREERQNIVLWRQPLITLQYFSLEILVILKEWTSKLWHRQSIVVSFLLLLAV 88

Query: 78  AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
             I    +EG H+++V+ I K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI   T+
Sbjct: 89  L-IATYYVEGVHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTL 148

Query: 138 KAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRVPLSSILPMVQIEAILWG 197
            A +C  V+     Y    +       E              + L SI+  V+IEA +WG
Sbjct: 149 AAYECNSVNFPEPPYPDQIICPDEEGTE------------GTISLWSIISKVRIEACMWG 208

Query: 198 IGTALGELPPYFISRAARLSGGR--SEAMEELDASSRETNGFIPTYLNRVKRWFLSHAQH 257
           IGTA+GELPPYF++RAARLSG     E  +E +            + +R K       Q 
Sbjct: 209 IGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAES-AQDFASRAKLAVQKLVQK 268

Query: 258 LNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNN 317
           + F  IL  AS+PNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I   + 
Sbjct: 269 VGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSK 328

Query: 318 QLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAATNVKAKWDFSVSSI 377
            +++    +++  +  VPG    L +   +    + + L   S   T     W   +S +
Sbjct: 329 HIVE----QMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGTPQGENW---LSWM 388

Query: 378 WNTIVWLMIMNFFVKIMTSTAQRYLKQQQ 405
           +  +V +M+  F + I+ S AQ Y K+ Q
Sbjct: 389 FEKLVVVMVCYFILSIINSMAQSYAKRIQ 396

BLAST of CmaCh17G002440 vs. ExPASy TrEMBL
Match: A0A6J1KZC5 (vacuole membrane protein KMS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498967 PE=4 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.2e-239
Identity = 422/422 (100.00%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY
Sbjct: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT
Sbjct: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360

Query: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC 420
           ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC
Sbjct: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC 420

Query: 421 SN 423
           SN
Sbjct: 421 SN 422

BLAST of CmaCh17G002440 vs. ExPASy TrEMBL
Match: A0A6J1H384 (vacuole membrane protein KMS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460087 PE=4 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.3e-234
Identity = 414/422 (98.10%), Postives = 416/422 (98.58%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASI SHDSEVSISEL RKQKEELENLTLVTQPFKTLKFFILGVLQYSK SIAY
Sbjct: 1   MGTVEEAASIASHDSEVSISELSRKQKEELENLTLVTQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTS+V
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEEL+ASSRETNGFIPT
Sbjct: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELNASSRETNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360

Query: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC 420
           ATNVKAKWDFSVSSIWNTIVWLMIMNF VKIMTSTAQRYLKQQQDEEL ALTNKVPA AC
Sbjct: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFSVKIMTSTAQRYLKQQQDEELAALTNKVPASAC 420

Query: 421 SN 423
           SN
Sbjct: 421 SN 422

BLAST of CmaCh17G002440 vs. ExPASy TrEMBL
Match: A0A0A0KED1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G424010 PE=4 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.4e-221
Identity = 394/424 (92.92%), Postives = 406/424 (95.75%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASI SHDSEVSISELR+KQK+ELENLTLV QPFKTLKFFILGVLQYSK SIAY
Sbjct: 1   MGTVEEAASIASHDSEVSISELRKKQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGW LLL+LIIG AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEK+CSEFGP +FTS+V
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKECSEFGPPLFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQ+EAILWGIGTALGELPPYFISRAARLSG RSEAMEELDASSRE+NGFIPT
Sbjct: 181 PLSSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLP LI+KLNAIKAKYLKAPS A
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPGLIAKLNAIKAKYLKAPSQA 360

Query: 361 -ATNVKA-KWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPAL 420
             TN K  KWDFSVSSIWNTIVWLMIMNF +KIMTSTAQRYLK+QQD+EL ALTNKVPA 
Sbjct: 361 MTTNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKVPAS 420

Query: 421 ACSN 423
            CSN
Sbjct: 421 TCSN 424

BLAST of CmaCh17G002440 vs. ExPASy TrEMBL
Match: A0A5A7V7Q6 (Vacuole membrane protein KMS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold75860G00460 PE=4 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.3e-219
Identity = 391/424 (92.22%), Postives = 404/424 (95.28%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASI SHDSEV  S+LRR+QK+ELENLTLV QPFKTLKFFILGVLQYSK SIAY
Sbjct: 1   MGTVEEAASIASHDSEV--SKLRRQQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGW LLL+LIIG AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGP +FTSRV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPPLFTSRV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQ+EAILWGIGTALGELPPYFISRAARLSG RSEAMEELDASSRE+NGFIPT
Sbjct: 181 PLSSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNR+KRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRMKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSS LP L++KLNAIKAKYLKAPSHA
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSALPGLVAKLNAIKAKYLKAPSHA 360

Query: 361 -ATNVKA-KWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPAL 420
             TN K  KWDFSVSSIWNTIVWLMIMNF +KIMTSTAQRYLK+QQD+EL ALTNKVPA 
Sbjct: 361 ITTNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKVPAS 420

Query: 421 ACSN 423
            CSN
Sbjct: 421 TCSN 422

BLAST of CmaCh17G002440 vs. ExPASy TrEMBL
Match: A0A1S3BMX8 (vacuole membrane protein KMS1 OS=Cucumis melo OX=3656 GN=LOC103491404 PE=4 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.3e-219
Identity = 391/424 (92.22%), Postives = 404/424 (95.28%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASI SHDSEV  S+LRR+QK+ELENLTLV QPFKTLKFFILGVLQYSK SIAY
Sbjct: 1   MGTVEEAASIASHDSEV--SKLRRQQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGW LLL+LIIG AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGP +FTSRV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPPLFTSRV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQ+EAILWGIGTALGELPPYFISRAARLSG RSEAMEELDASSRE+NGFIPT
Sbjct: 181 PLSSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNR+KRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRMKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSS LP L++KLNAIKAKYLKAPSHA
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSALPGLVAKLNAIKAKYLKAPSHA 360

Query: 361 -ATNVKA-KWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPAL 420
             TN K  KWDFSVSSIWNTIVWLMIMNF +KIMTSTAQRYLK+QQD+EL ALTNKVPA 
Sbjct: 361 ITTNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKVPAS 420

Query: 421 ACSN 423
            CSN
Sbjct: 421 TCSN 422

BLAST of CmaCh17G002440 vs. NCBI nr
Match: XP_023006134.1 (vacuole membrane protein KMS1-like [Cucurbita maxima] >XP_023006135.1 vacuole membrane protein KMS1-like [Cucurbita maxima])

HSP 1 Score: 838.6 bits (2165), Expect = 2.4e-239
Identity = 422/422 (100.00%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY
Sbjct: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT
Sbjct: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360

Query: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC 420
           ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC
Sbjct: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC 420

Query: 421 SN 423
           SN
Sbjct: 421 SN 422

BLAST of CmaCh17G002440 vs. NCBI nr
Match: XP_023548712.1 (vacuole membrane protein KMS1-like [Cucurbita pepo subsp. pepo] >XP_023548714.1 vacuole membrane protein KMS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 826.2 bits (2133), Expect = 1.2e-235
Identity = 417/422 (98.82%), Postives = 417/422 (98.82%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASI SHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSK SIAY
Sbjct: 1   MGTVEEAASIASHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT
Sbjct: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360

Query: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC 420
           ATNVKAKWDFSVSSIWNTIVWLMIMNF VKIMTSTAQRYLKQQQDEEL ALTNKVPA AC
Sbjct: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFSVKIMTSTAQRYLKQQQDEELAALTNKVPASAC 420

Query: 421 SN 423
           SN
Sbjct: 421 SN 422

BLAST of CmaCh17G002440 vs. NCBI nr
Match: KAG6574990.1 (Vacuole membrane protein KMS1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013560.1 Vacuole membrane protein KMS1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 824.7 bits (2129), Expect = 3.6e-235
Identity = 416/422 (98.58%), Postives = 417/422 (98.82%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASI SHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSK SIAY
Sbjct: 1   MGTVEEAASIASHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTS+V
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT
Sbjct: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360

Query: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC 420
           ATNVKAKWDFSVSSIWNTIVWLMIMNF VKIMTSTAQRYLKQQQDEEL ALTNKVPA AC
Sbjct: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFSVKIMTSTAQRYLKQQQDEELAALTNKVPASAC 420

Query: 421 SN 423
           SN
Sbjct: 421 SN 422

BLAST of CmaCh17G002440 vs. NCBI nr
Match: XP_022958951.1 (vacuole membrane protein KMS1-like [Cucurbita moschata] >XP_022958952.1 vacuole membrane protein KMS1-like [Cucurbita moschata])

HSP 1 Score: 820.5 bits (2118), Expect = 6.8e-234
Identity = 414/422 (98.10%), Postives = 416/422 (98.58%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASI SHDSEVSISEL RKQKEELENLTLVTQPFKTLKFFILGVLQYSK SIAY
Sbjct: 1   MGTVEEAASIASHDSEVSISELSRKQKEELENLTLVTQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTS+V
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEEL+ASSRETNGFIPT
Sbjct: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELNASSRETNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360

Query: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALAC 420
           ATNVKAKWDFSVSSIWNTIVWLMIMNF VKIMTSTAQRYLKQQQDEEL ALTNKVPA AC
Sbjct: 361 ATNVKAKWDFSVSSIWNTIVWLMIMNFSVKIMTSTAQRYLKQQQDEELAALTNKVPASAC 420

Query: 421 SN 423
           SN
Sbjct: 421 SN 422

BLAST of CmaCh17G002440 vs. NCBI nr
Match: XP_004140147.1 (vacuole membrane protein KMS1 [Cucumis sativus] >KGN48010.1 hypothetical protein Csa_003480 [Cucumis sativus])

HSP 1 Score: 778.5 bits (2009), Expect = 3.0e-221
Identity = 394/424 (92.92%), Postives = 406/424 (95.75%), Query Frame = 0

Query: 1   MGTVEEAASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAY 60
           MGTVEEAASI SHDSEVSISELR+KQK+ELENLTLV QPFKTLKFFILGVLQYSK SIAY
Sbjct: 1   MGTVEEAASIASHDSEVSISELRKKQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAY 60

Query: 61  ILAKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120
           ILAKGGW LLL+LIIG AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG
Sbjct: 61  ILAKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSG 120

Query: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRV 180
           LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEK+CSEFGP +FTS+V
Sbjct: 121 LHTFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKECSEFGPPLFTSQV 180

Query: 181 PLSSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPT 240
           PLSSILPMVQ+EAILWGIGTALGELPPYFISRAARLSG RSEAMEELDASSRE+NGFIPT
Sbjct: 181 PLSSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPT 240

Query: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300
           YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII
Sbjct: 241 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 300

Query: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHA 360
           KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLP LI+KLNAIKAKYLKAPS A
Sbjct: 301 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPGLIAKLNAIKAKYLKAPSQA 360

Query: 361 -ATNVKA-KWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPAL 420
             TN K  KWDFSVSSIWNTIVWLMIMNF +KIMTSTAQRYLK+QQD+EL ALTNKVPA 
Sbjct: 361 MTTNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKVPAS 420

Query: 421 ACSN 423
            CSN
Sbjct: 421 TCSN 424

BLAST of CmaCh17G002440 vs. TAIR 10
Match: AT4G14950.1 (SNARE associated Golgi protein family )

HSP 1 Score: 587.0 bits (1512), Expect = 1.2e-167
Identity = 283/412 (68.69%), Postives = 344/412 (83.50%), Query Frame = 0

Query: 7   AASITSHDSEVSISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGG 66
           +A + S  S+V+IS LR K ++E+ENLTL TQP  TLK F+   +QY K SI+Y+LA GG
Sbjct: 3   SAGVASSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGG 62

Query: 67  WFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVL 126
           WF+L+  ++ V+G L++T++GPH KHVEE+++Y  YGLWW+ LGVASSIGLGSGLHTFVL
Sbjct: 63  WFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVL 122

Query: 127 YLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFT----SRVPL 186
           YLGPHI  FT+KA  CGRVDLKSA YDTIQLKR PSWL+K CSEFGP +      SRVPL
Sbjct: 123 YLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPL 182

Query: 187 SSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYL 246
           +SILP VQ+EAILWGIGTALGELPPYFISRAA +SG   + MEELD SS E +GF+ T+L
Sbjct: 183 TSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHL 242

Query: 247 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 306
           NRVKRW L+H+QHLNF T+L+LASVPNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKT
Sbjct: 243 NRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKT 302

Query: 307 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAAT 366
           HIQTIFII VCNNQLLDW+ENELIWILS VPG +S+LP L +KL+A+K KY+ APS   +
Sbjct: 303 HIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPS 362

Query: 367 NVKA-KWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTN 414
           ++K  KWDFS +SIWN IVWLM++NFFVKI+T+TAQR+LK++Q++E+  LT+
Sbjct: 363 HIKVKKWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATLTH 414

BLAST of CmaCh17G002440 vs. TAIR 10
Match: AT4G14950.3 (SNARE associated Golgi protein family )

HSP 1 Score: 577.0 bits (1486), Expect = 1.2e-164
Identity = 278/401 (69.33%), Postives = 336/401 (83.79%), Query Frame = 0

Query: 18  SISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGGWFLLLHLIIGV 77
           S + LR K ++E+ENLTL TQP  TLK F+   +QY K SI+Y+LA GGWF+L+  ++ V
Sbjct: 4   SQATLREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILITTLLVV 63

Query: 78  AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
           +G L++T++GPH KHVEE+++Y  YGLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FT+
Sbjct: 64  SGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPHIALFTL 123

Query: 138 KAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFT----SRVPLSSILPMVQIEA 197
           KA  CGRVDLKSA YDTIQLKR PSWL+K CSEFGP +      SRVPL+SILP VQ+EA
Sbjct: 124 KATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILPQVQLEA 183

Query: 198 ILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYLNRVKRWFLSHA 257
           ILWGIGTALGELPPYFISRAA +SG   + MEELD SS E +GF+ T+LNRVKRW L+H+
Sbjct: 184 ILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKRWLLTHS 243

Query: 258 QHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVC 317
           QHLNF T+L+LASVPNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKTHIQTIFII VC
Sbjct: 244 QHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTIFIICVC 303

Query: 318 NNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAATNVKA-KWDFSV 377
           NNQLLDW+ENELIWILS VPG +S+LP L +KL+A+K KY+ APS   +++K  KWDFS 
Sbjct: 304 NNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPSHIKVKKWDFSF 363

Query: 378 SSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTN 414
           +SIWN IVWLM++NFFVKI+T+TAQR+LK++Q++E+  LT+
Sbjct: 364 ASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATLTH 404

BLAST of CmaCh17G002440 vs. TAIR 10
Match: AT1G05360.1 (BEST Arabidopsis thaliana protein match is: SNARE associated Golgi protein family (TAIR:AT4G14950.1); Has 332 Blast hits to 326 proteins in 115 species: Archae - 0; Bacteria - 11; Metazoa - 174; Fungi - 0; Plants - 74; Viruses - 0; Other Eukaryotes - 73 (source: NCBI BLink). )

HSP 1 Score: 545.8 bits (1405), Expect = 3.0e-155
Identity = 271/399 (67.92%), Postives = 322/399 (80.70%), Query Frame = 0

Query: 18  SISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGGWFLLLHLIIGV 77
           +IS LR K +++LE LTL +QPFKTL+ F++ V  Y +   +Y+LA  GW +L   I   
Sbjct: 13  AISGLREKHQQDLEKLTLTSQPFKTLRLFVVAVFLYVRRWSSYLLANVGWLILFCSIFVA 72

Query: 78  AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
              L++T++GPH KHVEE+ +Y  +GLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FTI
Sbjct: 73  FAALLVTLDGPHVKHVEELSEYTRFGLWWIFLGVASSIGLGSGLHTFVLYLGPHIALFTI 132

Query: 138 KAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRVPLSSILPMVQIEAILWG 197
           K +QCGRVDLKSA YDTIQLKR PSWL+K C EFG  VF+S VPLSSILP VQIEAILWG
Sbjct: 133 KVMQCGRVDLKSAIYDTIQLKRSPSWLDKPCHEFGSPVFSSGVPLSSILPQVQIEAILWG 192

Query: 198 IGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYLNRVKRWFLSHAQHLN 257
           +GTALGELPPYFISRAA LSGG+   M+EL+  S + NGFI   +N++K W LSH+Q+LN
Sbjct: 193 LGTALGELPPYFISRAASLSGGK---MKELETCSGDDNGFIAKRVNQIKSWLLSHSQYLN 252

Query: 258 FLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNNQL 317
           F TIL+LASVPNPLFDLAGIMCGQF  PFWEFF ATL+GKAIIKTHIQT+FII VCNNQL
Sbjct: 253 FFTILILASVPNPLFDLAGIMCGQFEKPFWEFFLATLIGKAIIKTHIQTVFIICVCNNQL 312

Query: 318 LDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKA--PSHAATNVKAKWDFSVSSI 377
           LDW+ENELI+ILSFVPGF+S LPEL +KL  +K KYL A  P  +  NVK KWD S +S+
Sbjct: 313 LDWVENELIYILSFVPGFASALPELTAKLRLMKEKYLIASPPVSSDINVK-KWDLSFASV 372

Query: 378 WNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNK 415
           WN +VWLM++NFF +I+TSTAQRYLK+QQ+EEL ALTNK
Sbjct: 373 WNGVVWLMLLNFFGQIVTSTAQRYLKKQQEEELDALTNK 407

BLAST of CmaCh17G002440 vs. TAIR 10
Match: AT4G14950.2 (SNARE associated Golgi protein family )

HSP 1 Score: 515.0 bits (1325), Expect = 5.7e-146
Identity = 247/352 (70.17%), Postives = 293/352 (83.24%), Query Frame = 0

Query: 18  SISELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYILAKGGWFLLLHLIIGV 77
           S + LR K ++E+ENLTL TQP  TLK F+   +QY K SI+Y+LA GGWF+L+  ++ V
Sbjct: 4   SQATLREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILITTLLVV 63

Query: 78  AGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
           +G L++T++GPH KHVEE+++Y  YGLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FT+
Sbjct: 64  SGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPHIALFTL 123

Query: 138 KAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFT----SRVPLSSILPMVQIEA 197
           KA  CGRVDLKSA YDTIQLKR PSWL+K CSEFGP +      SRVPL+SILP VQ+EA
Sbjct: 124 KATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILPQVQLEA 183

Query: 198 ILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYLNRVKRWFLSHA 257
           ILWGIGTALGELPPYFISRAA +SG   + MEELD SS E +GF+ T+LNRVKRW L+H+
Sbjct: 184 ILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKRWLLTHS 243

Query: 258 QHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVC 317
           QHLNF T+L+LASVPNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKTHIQTIFII VC
Sbjct: 244 QHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTIFIICVC 303

Query: 318 NNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAATNVK 366
           NNQLLDW+ENELIWILS VPG +S+LP L +KL+A+K KY+ APS   +++K
Sbjct: 304 NNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPSHIK 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5XF361.7e-16668.69Vacuole membrane protein KMS1 OS=Arabidopsis thaliana OX=3702 GN=KMS1 PE=1 SV=1[more]
F4I8Q74.2e-15467.92Vacuole membrane protein KMS2 OS=Arabidopsis thaliana OX=3702 GN=KMS2 PE=1 SV=1[more]
Q5R9K47.5e-5836.50Vacuole membrane protein 1 OS=Pongo abelii OX=9601 GN=VMP1 PE=2 SV=1[more]
Q99KU02.8e-5736.50Vacuole membrane protein 1 OS=Mus musculus OX=10090 GN=Vmp1 PE=1 SV=2[more]
Q96GC93.7e-5735.99Vacuole membrane protein 1 OS=Homo sapiens OX=9606 GN=VMP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KZC51.2e-239100.00vacuole membrane protein KMS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498967 P... [more]
A0A6J1H3843.3e-23498.10vacuole membrane protein KMS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460087... [more]
A0A0A0KED11.4e-22192.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G424010 PE=4 SV=1[more]
A0A5A7V7Q61.3e-21992.22Vacuole membrane protein KMS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BMX81.3e-21992.22vacuole membrane protein KMS1 OS=Cucumis melo OX=3656 GN=LOC103491404 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_023006134.12.4e-239100.00vacuole membrane protein KMS1-like [Cucurbita maxima] >XP_023006135.1 vacuole me... [more]
XP_023548712.11.2e-23598.82vacuole membrane protein KMS1-like [Cucurbita pepo subsp. pepo] >XP_023548714.1 ... [more]
KAG6574990.13.6e-23598.58Vacuole membrane protein KMS1, partial [Cucurbita argyrosperma subsp. sororia] >... [more]
XP_022958951.16.8e-23498.10vacuole membrane protein KMS1-like [Cucurbita moschata] >XP_022958952.1 vacuole ... [more]
XP_004140147.13.0e-22192.92vacuole membrane protein KMS1 [Cucumis sativus] >KGN48010.1 hypothetical protein... [more]
Match NameE-valueIdentityDescription
AT4G14950.11.2e-16768.69SNARE associated Golgi protein family [more]
AT4G14950.31.2e-16469.33SNARE associated Golgi protein family [more]
AT1G05360.13.0e-15567.92BEST Arabidopsis thaliana protein match is: SNARE associated Golgi protein famil... [more]
AT4G14950.25.7e-14670.17SNARE associated Golgi protein family [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032816SNARE associated Golgi proteinPFAMPF09335SNARE_assoccoord: 198..299
e-value: 1.1E-8
score: 35.5
NoneNo IPR availablePANTHERPTHR10281MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATEDcoord: 1..421
NoneNo IPR availablePANTHERPTHR10281:SF82VACUOLE MEMBRANE PROTEIN KMS1-LIKEcoord: 1..421

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G002440.1CmaCh17G002440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane