CmaCh17G002050 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G002050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionperakine reductase-like
LocationCma_Chr17: 1069081 .. 1069409 (+)
RNA-Seq ExpressionCmaCh17G002050
SyntenyCmaCh17G002050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGGAAAGGTGGTGTATTTTGAATAATTAACAACAAAAACTTTGACAAGTTATAGAGTTTGATTTGATTGACTGAAAGTTGTTTAAATTTGCAGGCTTTGAAACAGCTCCCTCGAGAGAAGATTCAGTTAGCTACAAAGTTTGGGATTGTACCATTAGGAGGATTTGAAATTGCAGTTAATGGAACGCCTGAATATGTAAGGAACTGCTGTGAAGCGAGTCTCAAGCACCTTCAAGTTGATTATATCGACCTCTACTATCAGGCACCGTGTCGACATATCTGTGCCAATAGAAGAAACTGTAAGTCGTCTTTAGGAGCAGAGTAA

mRNA sequence

ATGATTGGAAAGGTGGCTTTGAAACAGCTCCCTCGAGAGAAGATTCAGTTAGCTACAAAGTTTGGGATTGTACCATTAGGAGGATTTGAAATTGCAGTTAATGGAACGCCTGAATATGTAAGGAACTGCTGTGAAGCGAGTCTCAAGCACCTTCAAGTTGATTATATCGACCTCTACTATCAGGCACCGTGTCGACATATCTGTGCCAATAGAAGAAACTGTAAGTCGTCTTTAGGAGCAGAGTAA

Coding sequence (CDS)

ATGATTGGAAAGGTGGCTTTGAAACAGCTCCCTCGAGAGAAGATTCAGTTAGCTACAAAGTTTGGGATTGTACCATTAGGAGGATTTGAAATTGCAGTTAATGGAACGCCTGAATATGTAAGGAACTGCTGTGAAGCGAGTCTCAAGCACCTTCAAGTTGATTATATCGACCTCTACTATCAGGCACCGTGTCGACATATCTGTGCCAATAGAAGAAACTGTAAGTCGTCTTTAGGAGCAGAGTAA

Protein sequence

MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYYQAPCRHICANRRNCKSSLGAE
Homology
BLAST of CmaCh17G002050 vs. ExPASy Swiss-Prot
Match: C6TBN2 (Probable aldo-keto reductase 1 OS=Glycine max OX=3847 GN=AKR1 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 7.9e-17
Identity = 40/61 (65.57%), Postives = 49/61 (80.33%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           ++GK ALKQLPREKIQ+ATKFGI   G  ++ + G+PEYVR+CCE  LK L V+YIDLYY
Sbjct: 70  LVGK-ALKQLPREKIQIATKFGIASRGFPDMKIEGSPEYVRSCCETGLKRLDVEYIDLYY 129

Query: 61  Q 62
           Q
Sbjct: 130 Q 129

BLAST of CmaCh17G002050 vs. ExPASy Swiss-Prot
Match: Q3L181 (Perakine reductase OS=Rauvolfia serpentina OX=4060 GN=PR PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.3e-16
Identity = 40/60 (66.67%), Postives = 48/60 (80.00%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           ++GK ALKQLPREKIQ+ TKFGI  +G   +   GTP+YVR+CCEASLK L VDYIDL+Y
Sbjct: 66  LLGK-ALKQLPREKIQVGTKFGIHEIGFSGVKAKGTPDYVRSCCEASLKRLDVDYIDLFY 124

BLAST of CmaCh17G002050 vs. ExPASy Swiss-Prot
Match: Q84M96 (Probable aldo-keto reductase 2 OS=Arabidopsis thaliana OX=3702 GN=AGD2 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.2e-10
Identity = 34/61 (55.74%), Postives = 41/61 (67.21%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           ++GK ALK   +EK++LATKFG   + G    V G PEYVR  CEASLK L +  IDLYY
Sbjct: 71  LLGK-ALKDGVKEKVELATKFGFFIVEGEISEVRGDPEYVRAACEASLKRLDIACIDLYY 130

Query: 61  Q 62
           Q
Sbjct: 131 Q 130

BLAST of CmaCh17G002050 vs. ExPASy Swiss-Prot
Match: Q93ZN2 (Probable aldo-keto reductase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g60710 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 5.5e-10
Identity = 36/61 (59.02%), Postives = 42/61 (68.85%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           ++GK ALK   REK++LATKFGI    G +  V G PEYVR  CEASLK L +  IDLYY
Sbjct: 71  LLGK-ALKDGVREKVELATKFGISYAEG-KREVRGDPEYVRAACEASLKRLDIACIDLYY 129

Query: 61  Q 62
           Q
Sbjct: 131 Q 129

BLAST of CmaCh17G002050 vs. ExPASy Swiss-Prot
Match: F4HPY8 (Probable aldo-keto reductase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g60750 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 7.2e-10
Identity = 34/61 (55.74%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           ++GK ALK   R+K++LATKFGI      +    G PEYVR  CEASLK L V  IDLYY
Sbjct: 72  LLGK-ALKDGLRDKVELATKFGITASEDGKFGFRGDPEYVRIACEASLKRLGVTCIDLYY 131

Query: 61  Q 62
           Q
Sbjct: 132 Q 131

BLAST of CmaCh17G002050 vs. ExPASy TrEMBL
Match: A0A6J1H5Z2 (perakine reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111459922 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.2e-23
Identity = 58/61 (95.08%), Postives = 58/61 (95.08%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGIVPLGGFEIAV GTPEYVRNCCEASLK LQVDYIDLYY
Sbjct: 76  MIGK-ALKQLPREKIQLATKFGIVPLGGFEIAVKGTPEYVRNCCEASLKRLQVDYIDLYY 135

Query: 61  Q 62
           Q
Sbjct: 136 Q 135

BLAST of CmaCh17G002050 vs. ExPASy TrEMBL
Match: A0A0A0LKS7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G222020 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-23
Identity = 61/73 (83.56%), Postives = 62/73 (84.93%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGIVPLGG E AVNGTPEYVR CCEASL+ LQVDYIDLYY
Sbjct: 76  MIGK-ALKQLPREKIQLATKFGIVPLGGLEFAVNGTPEYVRKCCEASLERLQVDYIDLYY 135

Query: 61  QAPCRHICANRRN 74
           Q     I ANRRN
Sbjct: 136 Q---HRIDANRRN 144

BLAST of CmaCh17G002050 vs. ExPASy TrEMBL
Match: A0A5D3E2P5 (Perakine reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001380 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-21
Identity = 54/61 (88.52%), Postives = 56/61 (91.80%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGI+ LGGFE AVNGTPEYVR CCEASL+ LQVDYIDLYY
Sbjct: 58  MIGK-ALKQLPREKIQLATKFGIIRLGGFEFAVNGTPEYVRKCCEASLERLQVDYIDLYY 117

Query: 61  Q 62
           Q
Sbjct: 118 Q 117

BLAST of CmaCh17G002050 vs. ExPASy TrEMBL
Match: A0A5A7UV87 (Perakine reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G003330 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-21
Identity = 54/61 (88.52%), Postives = 56/61 (91.80%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGI+ LGGFE AVNGTPEYVR CCEASL+ LQVDYIDLYY
Sbjct: 76  MIGK-ALKQLPREKIQLATKFGIIRLGGFEFAVNGTPEYVRKCCEASLERLQVDYIDLYY 135

Query: 61  Q 62
           Q
Sbjct: 136 Q 135

BLAST of CmaCh17G002050 vs. ExPASy TrEMBL
Match: A0A1S3BL46 (perakine reductase-like OS=Cucumis melo OX=3656 GN=LOC103491226 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-21
Identity = 54/61 (88.52%), Postives = 56/61 (91.80%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGI+ LGGFE AVNGTPEYVR CCEASL+ LQVDYIDLYY
Sbjct: 76  MIGK-ALKQLPREKIQLATKFGIIRLGGFEFAVNGTPEYVRKCCEASLERLQVDYIDLYY 135

Query: 61  Q 62
           Q
Sbjct: 136 Q 135

BLAST of CmaCh17G002050 vs. NCBI nr
Match: KAE8651833.1 (hypothetical protein Csa_006558 [Cucumis sativus])

HSP 1 Score: 126.7 bits (317), Expect = 9.0e-26
Identity = 65/77 (84.42%), Postives = 66/77 (85.71%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGIVPLGG E AVNGTPEYVR CCEASL+ LQVDYIDLYY
Sbjct: 76  MIGK-ALKQLPREKIQLATKFGIVPLGGLEFAVNGTPEYVRKCCEASLERLQVDYIDLYY 135

Query: 61  QAPCRHICANRRNCKSS 78
           Q     I ANRRNCKSS
Sbjct: 136 Q---HRIDANRRNCKSS 148

BLAST of CmaCh17G002050 vs. NCBI nr
Match: KAG6574956.1 (putative aldo-keto reductase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013523.1 putative aldo-keto reductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 120.9 bits (302), Expect = 4.9e-24
Identity = 59/61 (96.72%), Postives = 59/61 (96.72%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLK LQVDYIDLYY
Sbjct: 76  MIGK-ALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKRLQVDYIDLYY 135

Query: 61  Q 62
           Q
Sbjct: 136 Q 135

BLAST of CmaCh17G002050 vs. NCBI nr
Match: XP_022958734.1 (perakine reductase-like [Cucurbita moschata])

HSP 1 Score: 118.6 bits (296), Expect = 2.5e-23
Identity = 58/61 (95.08%), Postives = 58/61 (95.08%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGIVPLGGFEIAV GTPEYVRNCCEASLK LQVDYIDLYY
Sbjct: 76  MIGK-ALKQLPREKIQLATKFGIVPLGGFEIAVKGTPEYVRNCCEASLKRLQVDYIDLYY 135

Query: 61  Q 62
           Q
Sbjct: 136 Q 135

BLAST of CmaCh17G002050 vs. NCBI nr
Match: XP_023547959.1 (perakine reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 118.2 bits (295), Expect = 3.2e-23
Identity = 57/61 (93.44%), Postives = 58/61 (95.08%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGI+PLGGFEIAV GTPEYVRNCCEASLK LQVDYIDLYY
Sbjct: 76  MIGK-ALKQLPREKIQLATKFGIIPLGGFEIAVKGTPEYVRNCCEASLKRLQVDYIDLYY 135

Query: 61  Q 62
           Q
Sbjct: 136 Q 135

BLAST of CmaCh17G002050 vs. NCBI nr
Match: XP_008449311.1 (PREDICTED: perakine reductase-like [Cucumis melo] >KAA0057455.1 perakine reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 111.7 bits (278), Expect = 3.0e-21
Identity = 54/61 (88.52%), Postives = 56/61 (91.80%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           MIGK ALKQLPREKIQLATKFGI+ LGGFE AVNGTPEYVR CCEASL+ LQVDYIDLYY
Sbjct: 76  MIGK-ALKQLPREKIQLATKFGIIRLGGFEFAVNGTPEYVRKCCEASLERLQVDYIDLYY 135

Query: 61  Q 62
           Q
Sbjct: 136 Q 135

BLAST of CmaCh17G002050 vs. TAIR 10
Match: AT1G60680.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 2.3e-11
Identity = 34/61 (55.74%), Postives = 41/61 (67.21%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           ++GK ALK   +EK++LATKFG   + G    V G PEYVR  CEASLK L +  IDLYY
Sbjct: 71  LLGK-ALKDGVKEKVELATKFGFFIVEGEISEVRGDPEYVRAACEASLKRLDIACIDLYY 130

Query: 61  Q 62
           Q
Sbjct: 131 Q 130

BLAST of CmaCh17G002050 vs. TAIR 10
Match: AT1G60710.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 3.9e-11
Identity = 36/61 (59.02%), Postives = 42/61 (68.85%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           ++GK ALK   REK++LATKFGI    G +  V G PEYVR  CEASLK L +  IDLYY
Sbjct: 71  LLGK-ALKDGVREKVELATKFGISYAEG-KREVRGDPEYVRAACEASLKRLDIACIDLYY 129

Query: 61  Q 62
           Q
Sbjct: 131 Q 129

BLAST of CmaCh17G002050 vs. TAIR 10
Match: AT1G60750.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 5.1e-11
Identity = 34/61 (55.74%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           ++GK ALK   R+K++LATKFGI      +    G PEYVR  CEASLK L V  IDLYY
Sbjct: 72  LLGK-ALKDGLRDKVELATKFGITASEDGKFGFRGDPEYVRIACEASLKRLGVTCIDLYY 131

Query: 61  Q 62
           Q
Sbjct: 132 Q 131

BLAST of CmaCh17G002050 vs. TAIR 10
Match: AT1G60690.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.9e-10
Identity = 35/61 (57.38%), Postives = 40/61 (65.57%), Query Frame = 0

Query: 1   MIGKVALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYY 60
           ++GK ALK   REK++LATKFGI    G    + G P YVR  CEASLK L V  IDLYY
Sbjct: 71  LLGK-ALKDGVREKVELATKFGISYAEG-NREIKGDPAYVRAACEASLKRLDVTCIDLYY 129

Query: 61  Q 62
           Q
Sbjct: 131 Q 129

BLAST of CmaCh17G002050 vs. TAIR 10
Match: AT1G10810.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 7.4e-10
Identity = 31/56 (55.36%), Postives = 38/56 (67.86%), Query Frame = 0

Query: 6   ALKQLPREKIQLATKFGIVPLGGFEIAVNGTPEYVRNCCEASLKHLQVDYIDLYYQ 62
           ALK   REK++LATKFG++ L   ++   G P YVR  CEASL+ L V  IDLYYQ
Sbjct: 75  ALKDGMREKVELATKFGLL-LKDQKLGYRGDPAYVRAACEASLRRLGVSCIDLYYQ 129

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C6TBN27.9e-1765.57Probable aldo-keto reductase 1 OS=Glycine max OX=3847 GN=AKR1 PE=2 SV=1[more]
Q3L1812.3e-1666.67Perakine reductase OS=Rauvolfia serpentina OX=4060 GN=PR PE=1 SV=1[more]
Q84M963.2e-1055.74Probable aldo-keto reductase 2 OS=Arabidopsis thaliana OX=3702 GN=AGD2 PE=2 SV=1[more]
Q93ZN25.5e-1059.02Probable aldo-keto reductase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g60710 PE=2... [more]
F4HPY87.2e-1055.74Probable aldo-keto reductase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g60750 PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1H5Z21.2e-2395.08perakine reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111459922 PE=4 SV=1[more]
A0A0A0LKS71.6e-2383.56Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G222020 PE=4 SV=1[more]
A0A5D3E2P51.5e-2188.52Perakine reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7UV871.5e-2188.52Perakine reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3BL461.5e-2188.52perakine reductase-like OS=Cucumis melo OX=3656 GN=LOC103491226 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAE8651833.19.0e-2684.42hypothetical protein Csa_006558 [Cucumis sativus][more]
KAG6574956.14.9e-2496.72putative aldo-keto reductase 1, partial [Cucurbita argyrosperma subsp. sororia] ... [more]
XP_022958734.12.5e-2395.08perakine reductase-like [Cucurbita moschata][more]
XP_023547959.13.2e-2393.44perakine reductase-like [Cucurbita pepo subsp. pepo][more]
XP_008449311.13.0e-2188.52PREDICTED: perakine reductase-like [Cucumis melo] >KAA0057455.1 perakine reducta... [more]
Match NameE-valueIdentityDescription
AT1G60680.12.3e-1155.74NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60710.13.9e-1159.02NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60750.15.1e-1155.74NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60690.11.9e-1057.38NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G10810.17.4e-1055.36NAD(P)-linked oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 1..68
e-value: 3.3E-15
score: 57.8
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILY51430NAD(P)-linked oxidoreductasecoord: 6..61
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 1..62
e-value: 1.7E-8
score: 34.1
NoneNo IPR availablePANTHERPTHR43625:SF54ALDO-KETO REDUCTASE 1-RELATEDcoord: 2..61
NoneNo IPR availablePANTHERPTHR43625AFLATOXIN B1 ALDEHYDE REDUCTASEcoord: 2..61

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G002050.1CmaCh17G002050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004033 aldo-keto reductase (NADP) activity
molecular_function GO:0047834 D-threo-aldose 1-dehydrogenase activity