CmaCh16G003030 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G003030
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPyruvate kinase
LocationCma_Chr16: 1410722 .. 1414871 (+)
RNA-Seq ExpressionCmaCh16G003030
SyntenyCmaCh16G003030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGGTAAATGCTATGATGATTCGTGCTGGTTGCAATTTTGTTGCGAAAAAGCCTGTATTAGATGTGAAGCGTTCGGGTTTTGGCATACCGGTTTTCGGTCGAACAGTTTGGTGCAATCGAAGGAGGACGTCGGGGAGAGTTGGAAATGGGGTGAAAGCGGTAATGCAAGTGGATTTGGAGGATGCGGAGAGATTGAGAAGATTGGAGGGCTTAGAAGCGACATTAGGGCTTGATGTTGTAGCGGAAAGGGAGTTGAAGGAGAAAGGATTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGCCCTGCGTGTAGTGCGATTGAGGATCTGGAGAAGTTGGCATTGGAAGGTATGAACATTGCGAGATTGAATATGTGCCACAATTCGAGAGAGTGGCATTGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGGGAAAGGATTCTGTATTTCGGTGATGATCGATACTGAAGGGAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCCATAAAAGTAGAGGTAAATGTTATGTCTCTGTTATTTTTTGCTGCTATTTAACTGAAATATGTTACTATGTTCACTGTTTTCTTCTGTAAATTAAGAACACTGAGTCTAAATTTTCTGGCGATAATTGTTCAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTATCGTTCATTCACTGTTCAAGCGAGCTATGAAGGTTTCTCTGAAGGTGTGTCTTGAATCTCTTGTTTCTTCGTCTTCTGAATTGTGATTAGAAGAAATATTTGAATCCCACAAGTTACTTAGAATATGAGTTTGATGTTGATGAGCTTTCTTTTACGAGTTTGGGCAGGTATTCAAGTGGGTGATTCAGTTGTAATTGATGGAGGAATGGCAACCTTTGAGGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAGTACAGCTTTTGGAGAGATGGGAGGGTTGTTGTGAGAAATCACGAGTCTCCAACGCTGTCGAGTAAGGTAGGAACGTGTCTCTGCTGCATATCAATACTCATTCTGAGAAATTTAGTTGTAATGAAATATGTGAGTTGATTTTAGTGTGAGATTTCATCTTGGCGATGAGAACGAAACATTTCTTATAAGGGGAGAAAATCTCTCCCTAGTAGACACGTTTTAAAACCGTGAATTTGATGACGATATATAATGGGCTAAAGCAGACAATATCTACTAGCAGTGGATTTGGGCTGTTGCGAATGGTATCAGAGCCAAATACTGAGCGGTGTGCTAACAAGGACGTTGGGCTACCAATGGGAGTGGATTGTGAGATTCCACATCAGTTGGAGAAGGGAAACCTCTCTCTAGTAAACACATTTTAAAACCATGAGGTTGACGGCGATACGAACGGGCAAAAGCGGACAATATCTGCTAGCGGTGGGTTTGGACTGTTACAAATGGTATTAGAGCCAAACACCCGGTGGTGTGCCAACAAGGACGTTGGCCCCCAAGAAGTGGATGGTGAGATTCCACATCGGTTGGAGAGGGGAACAAAGTATTCCTTGTAAGGGTGTAGAAACCTCAAAAGTATTCCTTGTAAGGGTGTAGAAACCTCTTTCTAGTAGACGCGTTTTAGAACCGTGAGCCTGATAGTGATACATAACGGGCCAAAGCGAACAATATCTACTAGCGGTGGGTTTGGGTGTTACATTTATGAAGTTGAAACTTTAAGATATGATGTATTTTATTGATTGGCCTTTTCATATTAATGCATAAACATGAGTAAAAGAATTCAATTAATCATGTCTTCTTGGATGATGCCGGATCTCTGCGTTTAAACCCTGTTTTTTCATGGTGTATTACAGGATTGGTCGGACATTGAATTTGGCATTGCGGAAGGCGTCAACTTCATTGCCCTTTCCTTCGTGAACGGTGCTGATCCTGTCAAGCATCTAAAGAACTACCTATCAACAAAATCAGCAAAGTAAGCATAAGAAGTCAACTTTTACCATGAATTGGGTTGAACTATCACTATCACTATCACTACCACTACTATGCATGAACGATAAATTATAGAGACATTCTTTTCCATAAAAATTTCCGGCAAAATATTGCTACTGAAACAGATATTTTGGGTAATTTCATAACTCAATTTAGTGATGAAGGGGGAAATCTGTTTTCATGCACAGATCCGTAAGGGTATTGGCTAAGATTGAAAGCTTGGAGTCTCTTCAGAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGAGATCTTGGGGTTGAAATTCCGCTGGAGCAGATACCAGCAATCCAAGAGGAAATAACTAGTGTTTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCCCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTATGAAAAATTTATCTTCGGTTTCTAATTTCAGGCATGTTTGGAATGATTTTCAAAATGTTGAAGACTATAATGATGATCCATTTTGGTGGGGTGTTGAATAGGTTGCAGATGTTTCGGAGGCTGTCCGACAGTATGCTGATGCATTAATGTTATCTGGGGAGTCTGCGATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTACAGATGGCCAGCTGCCGAATGGAGTTATGGAGTCGCGAAGAAAATAGGGAAAACTTCCTTCCCCACTATCAACTTGGGGTGTCATTGCACGATCGCATTGCAGAAGAAATTTGCAATTCTGCTTCTGAATTAGGTAAAACAGTTTGGTTTTTGGCTTTTATTCAATAATTCTTTATAGAATATATTGGCCTGTCTTAAATTCTAAAATATTTATATTTCAAGAATAGTCTTAAAACCTTCGTAAAAGTGAAGAGTAATTTTTACAAGCACAAGAATAGCTTCTAAAAGTCCATGAACCTGTGTGAAACTTGCGTATGGGAGGCCGTTAGAGATATTTAGTTTAGTTCCTAAATTTTTAGGTTTGTATTTATTGGTCGCGGGCCTTTCAAAAAAATGTCTAAAAGGTTCTTAAACTTTCAATTATGTATCCAATAAGATCTTGAACTTTAAAAAGTGTGTAATATATTCATAATCTTTCTTTTGTATGTTTGATAAGTTCATTAACTTTAAAAACAAATATGTGTATGATAAATCTTTTAATTGTGTATGATAAATCTTTTAATTTTCTAATTTTGTATTTAATAGGTCGTAGCATATTTTTAAGTTCGACGAATTAATTAGGCAAATGTTTTTGTGGTATAAAGCTGTTGTAGAACTGATCCTTGCACTTAGTTAGGTGTCAAGAAGTAAATTAATTTACCTTTGAACGAAAACTACTGTTTTGTTTGAGTGTTGCTAATTGCATTTGGCCCACGTTTCCACCAAAACAAACATCTCCATGTTGTTTTTGGCAGCTAACAGGCTTTCAGTAGACGCCATATTTGTGCTCACAACGCAAGGACACATGGCATCTCTTCTATCCCGCAACCGCCCAAGGCCGCCTATATTTGCATTGACCGATGATGATAGCACAAGAATGGCTTTGAATCTGCAATGGGGAGTGTTTCCTCTGCGCATTGAGCTCTCAGAAGACATTGAAGCAAACATCTGGAGGGGCATCGAGCTTGTGAAATCGAAGGGGTTGGTGAAACAGGGGGACTCTGTGTTGGTGGTCTCTGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAAACCATCGCGTAGGAGAAAGAGAAGAAGAGAACCAACCAATCCAACAAGAAACTTCAGTGATTTATGATTGCATGTACCATATGTGGAGGTTATTTATCTACTTATTATTTGTGGCCCTCTGTTTATAAAGACTACTTCGAAGCTTGGATAGGCCGTTTGTTGTTTTGTACTACTAGTAGCTAGTATTTGGTCATTTGAATAACTGTACTGAAATTGGGTATTCTGGATTTATTTGGTAGAAACAGGAGACGTAGCGAGTTAGGTTACTGAGAGAAATCTTCTTCCCTTAGTTTATTCCAATCGATTGAGTAGTCGTAGAAGTCGCATTCAAGCTACCGTCCTACTATAAGGATAATAAGCCATA

mRNA sequence

ATGGCGGAGGTAAATGCTATGATGATTCGTGCTGGTTGCAATTTTGTTGCGAAAAAGCCTGTATTAGATGTGAAGCGTTCGGGTTTTGGCATACCGGTTTTCGGTCGAACAGTTTGGTGCAATCGAAGGAGGACGTCGGGGAGAGTTGGAAATGGGGTGAAAGCGGTAATGCAAGTGGATTTGGAGGATGCGGAGAGATTGAGAAGATTGGAGGGCTTAGAAGCGACATTAGGGCTTGATGTTGTAGCGGAAAGGGAGTTGAAGGAGAAAGGATTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGCCCTGCGTGTAGTGCGATTGAGGATCTGGAGAAGTTGGCATTGGAAGGTATGAACATTGCGAGATTGAATATGTGCCACAATTCGAGAGAGTGGCATTGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGGGAAAGGATTCTGTATTTCGGTGATGATCGATACTGAAGGGAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCCATAAAAGTAGAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTATCGTTCATTCACTGTTCAAGCGAGCTATGAAGGTTTCTCTGAAGGTATTCAAGTGGGTGATTCAGTTGTAATTGATGGAGGAATGGCAACCTTTGAGGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAGTACAGCTTTTGGAGAGATGGGAGGGTTGTTGTGAGAAATCACGAGTCTCCAACGCTGTCGAGTAAGGATTGGTCGGACATTGAATTTGGCATTGCGGAAGGCGTCAACTTCATTGCCCTTTCCTTCGTGAACGGTGCTGATCCTGTCAAGCATCTAAAGAACTACCTATCAACAAAATCAGCAAAATCCGTAAGGGTATTGGCTAAGATTGAAAGCTTGGAGTCTCTTCAGAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGAGATCTTGGGGTTGAAATTCCGCTGGAGCAGATACCAGCAATCCAAGAGGAAATAACTAGTGTTTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCCCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTTGCAGATGTTTCGGAGGCTGTCCGACAGTATGCTGATGCATTAATGTTATCTGGGGAGTCTGCGATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTACAGATGGCCAGCTGCCGAATGGAGTTATGGAGTCGCGAAGAAAATAGGGAAAACTTCCTTCCCCACTATCAACTTGGGGTGTCATTGCACGATCGCATTGCAGAAGAAATTTGCAATTCTGCTTCTGAATTAGCTAACAGGCTTTCAGTAGACGCCATATTTGTGCTCACAACGCAAGGACACATGGCATCTCTTCTATCCCGCAACCGCCCAAGGCCGCCTATATTTGCATTGACCGATGATGATAGCACAAGAATGGCTTTGAATCTGCAATGGGGAGTGTTTCCTCTGCGCATTGAGCTCTCAGAAGACATTGAAGCAAACATCTGGAGGGGCATCGAGCTTGTGAAATCGAAGGGGTTGGTGAAACAGGGGGACTCTGTGTTGGTGGTCTCTGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAAACCATCGCGTAGGAGAAAGAGAAGAAGAGAACCAACCAATCCAACAAGAAACTTCAGTGATTTATGATTGCATGTACCATATGTGGAGGTTATTTATCTACTTATTATTTGTGGCCCTCTGTTTATAAAGACTACTTCGAAGCTTGGATAGGCCGTTTGTTGTTTTGTACTACTAGTAGCTAGTATTTGGTCATTTGAATAACTGTACTGAAATTGGGTATTCTGGATTTATTTGGTAGAAACAGGAGACGTAGCGAGTTAGGTTACTGAGAGAAATCTTCTTCCCTTAGTTTATTCCAATCGATTGAGTAGTCGTAGAAGTCGCATTCAAGCTACCGTCCTACTATAAGGATAATAAGCCATA

Coding sequence (CDS)

ATGGCGGAGGTAAATGCTATGATGATTCGTGCTGGTTGCAATTTTGTTGCGAAAAAGCCTGTATTAGATGTGAAGCGTTCGGGTTTTGGCATACCGGTTTTCGGTCGAACAGTTTGGTGCAATCGAAGGAGGACGTCGGGGAGAGTTGGAAATGGGGTGAAAGCGGTAATGCAAGTGGATTTGGAGGATGCGGAGAGATTGAGAAGATTGGAGGGCTTAGAAGCGACATTAGGGCTTGATGTTGTAGCGGAAAGGGAGTTGAAGGAGAAAGGATTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGCCCTGCGTGTAGTGCGATTGAGGATCTGGAGAAGTTGGCATTGGAAGGTATGAACATTGCGAGATTGAATATGTGCCACAATTCGAGAGAGTGGCATTGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGGGAAAGGATTCTGTATTTCGGTGATGATCGATACTGAAGGGAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCCATAAAAGTAGAGGATGGTTCAATCTGGTTGTTTACTGCTGAGAAATTTGAGGGCTATCGTTCATTCACTGTTCAAGCGAGCTATGAAGGTTTCTCTGAAGGTATTCAAGTGGGTGATTCAGTTGTAATTGATGGAGGAATGGCAACCTTTGAGGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAAGTGCACAGACCCTGGTTTGTTTCTTCCTCGAGCAAAGTACAGCTTTTGGAGAGATGGGAGGGTTGTTGTGAGAAATCACGAGTCTCCAACGCTGTCGAGTAAGGATTGGTCGGACATTGAATTTGGCATTGCGGAAGGCGTCAACTTCATTGCCCTTTCCTTCGTGAACGGTGCTGATCCTGTCAAGCATCTAAAGAACTACCTATCAACAAAATCAGCAAAATCCGTAAGGGTATTGGCTAAGATTGAAAGCTTGGAGTCTCTTCAGAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGAGATCTTGGGGTTGAAATTCCGCTGGAGCAGATACCAGCAATCCAAGAGGAAATAACTAGTGTTTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCCCAACTTCTAGAGTCGATGGTTGAATATCCTACTCCAACACGAGCTGAGGTTGCAGATGTTTCGGAGGCTGTCCGACAGTATGCTGATGCATTAATGTTATCTGGGGAGTCTGCGATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTACAGATGGCCAGCTGCCGAATGGAGTTATGGAGTCGCGAAGAAAATAGGGAAAACTTCCTTCCCCACTATCAACTTGGGGTGTCATTGCACGATCGCATTGCAGAAGAAATTTGCAATTCTGCTTCTGAATTAGCTAACAGGCTTTCAGTAGACGCCATATTTGTGCTCACAACGCAAGGACACATGGCATCTCTTCTATCCCGCAACCGCCCAAGGCCGCCTATATTTGCATTGACCGATGATGATAGCACAAGAATGGCTTTGAATCTGCAATGGGGAGTGTTTCCTCTGCGCATTGAGCTCTCAGAAGACATTGAAGCAAACATCTGGAGGGGCATCGAGCTTGTGAAATCGAAGGGGTTGGTGAAACAGGGGGACTCTGTGTTGGTGGTCTCTGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGTCCCAATCAATCCAGCTGAAAACCATCGCGTAG

Protein sequence

MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA
Homology
[Close]
BLAST of CmaCh16G003030 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 1.1e-168
Identity = 292/487 (59.96%), Postives = 369/487 (75.77%), Query Frame = 0 

Query: 79  LDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHC 138
           +D V E ELKE GF   R+TKL+CTIGPA    E LE LA+ GMN+ARLNMCH +R+WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYR-S 198
            VIR +++LNE KGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+  R  
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRVV-VRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKS-AKSV 318
           G +V  RN   PT+SSKDW DI+FGIAEGV+FIA+SFV  A+ + HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 RVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVI 378
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRM 438
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLS 498
           E W REE R   +P   +G S  D+I+EEICNSA+++AN L VDA+FV TT GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDS 558
           R RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+ +   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLVVSEI 563
           V+ VS++
Sbjct: 580 VIAVSDM 586

[Close]
BLAST of CmaCh16G003030 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.7e-167
Identity = 287/491 (58.45%), Postives = 374/491 (76.17%), Query Frame = 0 

Query: 75  ATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSR 134
           +++ +D V E ELKE GF   R+TKLVCTIGPA    E+LE LA+ GMN+AR+NMCH +R
Sbjct: 83  SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTR 142

Query: 135 EWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEG 194
           EWH  VI ++++LNE KGF +++M+DTEGS+IH+ D G  SS K EDG IW F+   ++ 
Sbjct: 143 EWHKSVIERVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS 202

Query: 195 YR-SFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYS 254
            R   T+  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ C+CTDPGL LPRA  +
Sbjct: 203 PRPERTINVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLT 262

Query: 255 FWRDGRVV-VRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSA 314
           FWRDG +V  RN   PT+SSKDW DI+FGIAEGV+FIA+SFV  A+ + HLK+Y++ +S 
Sbjct: 263 FWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSR 322

Query: 315 KS-VRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELN 374
            S + V+AKIES++SL+NLEEII ASDG MVARGDLG +IPLEQ+P+ Q+ I  VCR+LN
Sbjct: 323 DSDIAVIAKIESIDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLN 382

Query: 375 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 434
           KPVI+ASQLLESM+EYPTPTRAEVADVSEAVRQ ADALMLSGESA+G Y +KAL+VL+  
Sbjct: 383 KPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSV 442

Query: 435 SCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMA 494
           S R+E W REE     +    +G +  D I+EEICNSA+++AN L VDA+FV T  GHMA
Sbjct: 443 SVRIEKWWREEKHHEAMELPAIGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMA 502

Query: 495 SLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  ++D+E+N+ +   L+K++G++K
Sbjct: 503 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIK 562

Query: 555 QGDSVLVVSEI 563
            GD V+ VS++
Sbjct: 563 SGDLVIAVSDM 573

[Close]
BLAST of CmaCh16G003030 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 3.1e-163
Identity = 282/491 (57.43%), Postives = 371/491 (75.56%), Query Frame = 0 

Query: 75  ATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSR 134
           +++ +D V E ELKE GF   R+TKL+CTIGPA    E LE+LA  GMN+AR+NMCH +R
Sbjct: 93  SSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTR 152

Query: 135 EWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFE- 194
           EWH  VI ++++LNE KGF +++M+DTEGS+IH+ D G  SS K EDG IW FT   F+ 
Sbjct: 153 EWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP 212

Query: 195 GYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYS 254
                TV  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +
Sbjct: 213 PLPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLT 272

Query: 255 FWRDGRVV-VRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSA 314
           FWRDG++V  RN   PT+SSKDW DI+FGIAEGV+FIA+SFV  A+ +KHLK+Y+  ++ 
Sbjct: 273 FWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARAR 332

Query: 315 KS-VRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELN 374
            S + V+AKIES++SL+NLEEII+ASDG MVARGDLG +IPLEQ+P+ Q++I  +CR+LN
Sbjct: 333 DSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLN 392

Query: 375 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 434
           +PVI+ASQLLESM+EYP PTRAEVADVSEAVRQ  DALMLSGESA+G + +KAL+VL+  
Sbjct: 393 RPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSV 452

Query: 435 SCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMA 494
           S R+E   RE+ R   +    +  S  D I+EEICNSA+++AN L VDA+FV T  GHMA
Sbjct: 453 SLRIERMWREQKRHEVIELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMA 512

Query: 495 SLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVK 554
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+ +   L+K++G++K
Sbjct: 513 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIK 572

Query: 555 QGDSVLVVSEI 563
            GD ++ VS++
Sbjct: 573 SGDLIIAVSDM 583

[Close]
BLAST of CmaCh16G003030 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 8.9e-86
Identity = 187/465 (40.22%), Postives = 274/465 (58.92%), Query Frame = 0 

Query: 96  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLN-EGKGFC 155
           RKTK+VCTIGP+ S+ E + KLA  GMN+ARLNM H     H   I  +K+ N + +   
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 156 ISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVG 215
           I++M+DT+G +  V+    P  I +++G  + F+ ++       TV  +Y+ F   ++ G
Sbjct: 151 IAIMLDTKGPE--VISGDVPKPILLKEGQEFNFSIKRGVSTED-TVSVNYDDFINDVEAG 210

Query: 216 DSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKD 275
           D +++DGGM +  V  K  + + C+  D G    R      R   V  ++   P+++ KD
Sbjct: 211 DILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSR------RHLNVRGKSATLPSITEKD 270

Query: 276 WSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEII 335
           W DI+FG+   V+F A+SFV  A  V  LK+YL + +A  + V+ KIES +S+ NL  II
Sbjct: 271 WDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLKSCNA-DIHVIVKIESADSIPNLHSII 330

Query: 336 EASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAE 395
            ASDG MVARGDLG E+P+E++P +QE+I   C+ + KPVI+A+ +LESM+++PTPTRAE
Sbjct: 331 SASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAE 390

Query: 396 VADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLG 455
           V+D+S AVR+ ADA+MLSGE+A G Y  KA+ V+ + + R E       +    P     
Sbjct: 391 VSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTE---SSLQKSTSSPSQSAA 450

Query: 456 VSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRM 515
              H  + E     +S +AN LS   I V T  G MA +LS NRP   +FA T+++  + 
Sbjct: 451 YKSH--MGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNNERVKQ 510

Query: 516 ALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVV 560
            L L  GV P+ +E S D E    R I+L+ SK LVK G  V +V
Sbjct: 511 RLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLV 539

[Close]
BLAST of CmaCh16G003030 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.7e-84
Identity = 200/575 (34.78%), Postives = 310/575 (53.91%), Query Frame = 0 

Query: 24  VKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEATLGLDVVA 83
           +K + F + V G     N  + SGRV    + V+   +  A    R+E     +  + V 
Sbjct: 30  LKPASFAVKVLG-----NEAKRSGRVSVRSRRVVDTTVRSA----RVETEVIPVSPEDVP 89

Query: 84  ERELKEKGFLGM-----------------RKTKLVCTIGPACSAIEDLEKLALEGMNIAR 143
            RE + +  L M                 RKTK+VCT+GP+ +  E + KLA  GMN+AR
Sbjct: 90  NREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGPSTNTREMIWKLAEAGMNVAR 149

Query: 144 LNMCHNSREWHCDVIRKIKKLN-EGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIW 203
           +NM H     H  VI  +K+ N + K   I++M+DT+G ++   D   P  I ++ G  +
Sbjct: 150 MNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGD--LPQPIMLDPGQEF 209

Query: 204 LFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGL 263
            FT E+     S  V  +Y+ F   ++ GD +++DGGM +F V  K  + + C+  D G 
Sbjct: 210 TFTIERGVSTPS-CVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGE 269

Query: 264 FLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKN 323
              R      R   V  ++   P+++ KDW DI+FG+   V+F A+SFV  A  V  LK 
Sbjct: 270 LKSR------RHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKK 329

Query: 324 YLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITS 383
           YL    A  + V+ KIES +S+ NL  II ASDG MVARGDLG E+P+E++P +QEEI +
Sbjct: 330 YLQNSGA-DIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPILQEEIIN 389

Query: 384 VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKAL 443
           +CR + K VI+A+ +LESM+ +PTPTRAEV+D++ AVR+ ADA+MLSGE+A G +  KA 
Sbjct: 390 LCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAA 449

Query: 444 SVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLT 503
            V+   + R E           +P   LG +  + ++E     A+ ++N L    + V T
Sbjct: 450 GVMHTVALRTEATITSGE----MPP-NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFT 509

Query: 504 TQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVK 563
             G MA LLS  RP   I+A T++   +  L L  GV P+ +E ++D E      +  + 
Sbjct: 510 RTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALATLL 569

Query: 564 SKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
            +G+VK+G+ + +V   +     S ++ +IQ++ +
Sbjct: 570 KQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

[Close]
BLAST of CmaCh16G003030 vs. ExPASy TrEMBL
Match: A0A6J1J2T2 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482878 PE=3 SV=1)

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240
           DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC
Sbjct: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240

Query: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300
           TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV
Sbjct: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300

Query: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360
           KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ
Sbjct: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 582
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 581

[Close]
BLAST of CmaCh16G003030 vs. ExPASy TrEMBL
Match: A0A6J1E4Y3 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430639 PE=3 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 561/580 (96.72%), Postives = 572/580 (98.62%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAK PVLD+KRSGFG+PVFGRTVWCN RRT+GRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDLKRSGFGMPVFGRTVWCN-RRTTGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240
           DGSIWLFTAEKFEG R FTVQASYEGFSEGIQVGDS+VIDGGMATFEVIEKIGNDLSC+C
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300
           TDPGLFLPRAK SFWRDGRVVVRNHESPTLS KDWSDIEFGIAEGV+FIALSFVN ADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSRKDWSDIEFGIAEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360
           KHLKN+LSTK AKSVRVLAKIESLESLQNLEEIIEAS+GIMVARGDLGVEIPLEQIPA+Q
Sbjct: 301 KHLKNFLSTKPAKSVRVLAKIESLESLQNLEEIIEASEGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSA+ELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 579

[Close]
BLAST of CmaCh16G003030 vs. ExPASy TrEMBL
Match: A0A5A7TLC2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350 PE=3 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.7e-302
Identity = 530/581 (91.22%), Postives = 554/581 (95.35%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFG-RTVWCNRRRTSGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ + PVLDVKR GF +PV G RTVWCNRRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DL+D ERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE KGFCISVMID EGS+IHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEG R FTVQASYEGFSEGI+VGDS+VIDGGMATFEVIEKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADP 300
           CTDPGLFLPRAK SFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGV+FIALSFVN ADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAI 360
           V+HLKNYL TKSAKS+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVD 480
           YGQKALSVLQMAS RMELWSREEN +NFLP +QLGVSLHDRIAEEICNSA+ELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 581

[Close]
BLAST of CmaCh16G003030 vs. ExPASy TrEMBL
Match: A0A5D3E0J9 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00350 PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.5e-301
Identity = 529/581 (91.05%), Postives = 553/581 (95.18%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFG-RTVWCNRRRTSGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ + PVLDVKR GF +PV G RTVWCNRRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DL+D ERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE KGFCISVMID EGS+IHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEG R FTVQASYEGFSEGI+VGDS+VIDGGMATFEV EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADP 300
           CTDPGLFLPRAK SFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGV+FIALSFVN ADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAI 360
           V+HLKNYL TKSAKS+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVD 480
           YGQKALSVLQMAS RMELWSREEN +NFLP +QLGVSLHDRIAEEICNSA+ELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 581

[Close]
BLAST of CmaCh16G003030 vs. ExPASy TrEMBL
Match: E5GBH7 (Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.5e-301
Identity = 529/581 (91.05%), Postives = 553/581 (95.18%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFG-RTVWCNRRRTSGRVGNGVKAVMQV 60
           MAEVNAMMIRA CNF+ + PVLDVKR GF +PV G RTVWCNRRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
           DL+D ERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE KGFCISVMID EGS+IHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCK 240
           EDGSIWLFTAEKFEG R FTVQASYEGFSEGI+VGDS+VIDGGMATFEV EKIGNDLSC+
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADP 300
           CTDPGLFLPRAK SFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGV+FIALSFVN ADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAI 360
           V+HLKNYL TKSAKS+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVD 480
           YGQKALSVLQMAS RMELWSREEN +NFLP +QLGVSLHDRIAEEICNSA+ELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTI
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 581

[Close]
BLAST of CmaCh16G003030 vs. NCBI nr
Match: XP_022984667.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240
           DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC
Sbjct: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240

Query: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300
           TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV
Sbjct: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300

Query: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360
           KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ
Sbjct: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 582
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 581

[Close]
BLAST of CmaCh16G003030 vs. NCBI nr
Match: XP_023553283.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 568/581 (97.76%), Postives = 575/581 (98.97%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAK PVLDVKRSGFG+PVFGRTVWCNRRRT+GRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240
           DGSIWLFTAEKFEG R FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSC+C
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300
           TDPGLFLPRAK SFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGV+FIALSFVN ADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360
           KHLKNYLSTKSA+SVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+Q
Sbjct: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSL DRIAEEICNSA+ELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 582
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIA 581

[Close]
BLAST of CmaCh16G003030 vs. NCBI nr
Match: KAG7014927.1 (Plastidial pyruvate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 564/580 (97.24%), Postives = 572/580 (98.62%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAK PVLDVKRSGFG+PVFGRTVWCN RRT+GRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCN-RRTTGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWH DVIRKIKKLNEGKGFCISVMIDTEGSQ+HVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHRDVIRKIKKLNEGKGFCISVMIDTEGSQVHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240
           DGSIWLFTAEKFEG R FTVQASYEGFSEGIQVGDS+VIDGGMATFEVIEKIGNDLSC+C
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300
           TDPGLFLPRAK SFWRDGRVVVRNHESPTLSSKDWSDIEFGI EGV+FIALSFVN ADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIVEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360
           KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+Q
Sbjct: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSA+ELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 579

[Close]
BLAST of CmaCh16G003030 vs. NCBI nr
Match: XP_022922736.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 561/580 (96.72%), Postives = 572/580 (98.62%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60
           MAEVNAMMIRAGCNFVAK PVLD+KRSGFG+PVFGRTVWCN RRT+GRVGNGVKAVMQVD
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDLKRSGFGMPVFGRTVWCN-RRTTGRVGNGVKAVMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDAERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240
           DGSIWLFTAEKFEG R FTVQASYEGFSEGIQVGDS+VIDGGMATFEVIEKIGNDLSC+C
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300
           TDPGLFLPRAK SFWRDGRVVVRNHESPTLS KDWSDIEFGIAEGV+FIALSFVN ADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSRKDWSDIEFGIAEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360
           KHLKN+LSTK AKSVRVLAKIESLESLQNLEEIIEAS+GIMVARGDLGVEIPLEQIPA+Q
Sbjct: 301 KHLKNFLSTKPAKSVRVLAKIESLESLQNLEEIIEASEGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480
           GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSA+ELANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 579

[Close]
BLAST of CmaCh16G003030 vs. NCBI nr
Match: XP_038905899.1 (plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1053.1 bits (2722), Expect = 8.6e-304
Identity = 536/580 (92.41%), Postives = 557/580 (96.03%), Query Frame = 0 

Query: 1   MAEVNAMMIRAGCNFVAKKPVLDVKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVD 60
           MAEVNA+MIR+  +F+AK PVLDVKRSGFG+PV GRTVWCNRRRT G VGNGVKA MQVD
Sbjct: 1   MAEVNAIMIRSRFDFIAKSPVLDVKRSGFGMPVIGRTVWCNRRRTRGGVGNGVKATMQVD 60

Query: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LED ERL+RLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPAC+AIEDLEKLALE
Sbjct: 61  LEDEERLKRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACNAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180
           GMNIARLNMCHNSREWHCDVIRKIKKLNE KGFCISVMIDTEGSQIHVVDHGAPSS+KVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEKKGFCISVMIDTEGSQIHVVDHGAPSSVKVE 180

Query: 181 DGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKC 240
           DGSIWLFTAEKFEG R FTVQASYEGFSEGIQVGDS+VIDGGMATFEVIEKIGNDLSC+C
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPV 300
           TDPGLFLPRAK SFWRDGR+VVRNHESPTLSSKDWSDI+FGI+EGV+FIALSFVN ADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIKFGISEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQ 360
           +HLKNYL TKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPA+Q
Sbjct: 301 QHLKNYLLTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDA 480
           GQKALSVLQMAS RME WSREENR+NFLP +QLGVSLHDRIAEEICNSA+ELANRLSVDA
Sbjct: 421 GQKALSVLQMASSRMESWSREENRKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540
           IFVLT QGHMASLLSRNRP P IFALTDDDSTRMALNLQWGV PLRI+ SEDIEANI RG
Sbjct: 481 IFVLTNQGHMASLLSRNRPSPLIFALTDDDSTRMALNLQWGVVPLRIDFSEDIEANISRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           IELVKSKGLVK+GDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 IELVKSKGLVKKGDSVLVVSEISPARAASMASQSIQLKTI 580

[Close]
BLAST of CmaCh16G003030 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 594.7 bits (1532), Expect = 7.8e-170
Identity = 292/487 (59.96%), Postives = 369/487 (75.77%), Query Frame = 0 

Query: 79  LDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHC 138
           +D V E ELKE GF   R+TKL+CTIGPA    E LE LA+ GMN+ARLNMCH +R+WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 139 DVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYR-S 198
            VIR +++LNE KGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+  R  
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 199 FTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRD 258
            T+  SY+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 259 GRVV-VRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKS-AKSV 318
           G +V  RN   PT+SSKDW DI+FGIAEGV+FIA+SFV  A+ + HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 319 RVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVI 378
            V+AKIES++SL NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 379 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRM 438
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 439 ELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLS 498
           E W REE R   +P   +G S  D+I+EEICNSA+++AN L VDA+FV TT GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 499 RNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDS 558
           R RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+ +   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 559 VLVVSEI 563
           V+ VS++
Sbjct: 580 VIAVSDM 586

[Close]
BLAST of CmaCh16G003030 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 313.9 bits (803), Expect = 2.7e-85
Identity = 200/575 (34.78%), Postives = 310/575 (53.91%), Query Frame = 0 

Query: 24  VKRSGFGIPVFGRTVWCNRRRTSGRVGNGVKAVMQVDLEDAERLRRLEGLEATLGLDVVA 83
           +K + F + V G     N  + SGRV    + V+   +  A    R+E     +  + V 
Sbjct: 30  LKPASFAVKVLG-----NEAKRSGRVSVRSRRVVDTTVRSA----RVETEVIPVSPEDVP 89

Query: 84  ERELKEKGFLGM-----------------RKTKLVCTIGPACSAIEDLEKLALEGMNIAR 143
            RE + +  L M                 RKTK+VCT+GP+ +  E + KLA  GMN+AR
Sbjct: 90  NREEQLERLLEMQQFGDTSVGMWSKPTVRRKTKIVCTVGPSTNTREMIWKLAEAGMNVAR 149

Query: 144 LNMCHNSREWHCDVIRKIKKLN-EGKGFCISVMIDTEGSQIHVVDHGAPSSIKVEDGSIW 203
           +NM H     H  VI  +K+ N + K   I++M+DT+G ++   D   P  I ++ G  +
Sbjct: 150 MNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGD--LPQPIMLDPGQEF 209

Query: 204 LFTAEKFEGYRSFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCKCTDPGL 263
            FT E+     S  V  +Y+ F   ++ GD +++DGGM +F V  K  + + C+  D G 
Sbjct: 210 TFTIERGVSTPS-CVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGE 269

Query: 264 FLPRAKYSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVNFIALSFVNGADPVKHLKN 323
              R      R   V  ++   P+++ KDW DI+FG+   V+F A+SFV  A  V  LK 
Sbjct: 270 LKSR------RHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKK 329

Query: 324 YLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITS 383
           YL    A  + V+ KIES +S+ NL  II ASDG MVARGDLG E+P+E++P +QEEI +
Sbjct: 330 YLQNSGA-DIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAELPIEEVPILQEEIIN 389

Query: 384 VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKAL 443
           +CR + K VI+A+ +LESM+ +PTPTRAEV+D++ AVR+ ADA+MLSGE+A G +  KA 
Sbjct: 390 LCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAA 449

Query: 444 SVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSASELANRLSVDAIFVLT 503
            V+   + R E           +P   LG +  + ++E     A+ ++N L    + V T
Sbjct: 450 GVMHTVALRTEATITSGE----MPP-NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFT 509

Query: 504 TQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRGIELVK 563
             G MA LLS  RP   I+A T++   +  L L  GV P+ +E ++D E      +  + 
Sbjct: 510 RTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEFTDDAEETFANALATLL 569

Query: 564 SKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
            +G+VK+G+ + +V   +     S ++ +IQ++ +
Sbjct: 570 KQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

[Close]
BLAST of CmaCh16G003030 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 310.5 bits (794), Expect = 2.9e-84
Identity = 190/486 (39.09%), Postives = 279/486 (57.41%), Query Frame = 0 

Query: 96  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNE-GKGFC 155
           RKTK+VCTIGP+ S+ E + KLA  GMN+ARLNM H     H   I  +K+ N       
Sbjct: 99  RKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDKA 158

Query: 156 ISVMIDTEGSQIHVVDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVG 215
           I++M+DT+G ++   D   P  I +E+G  + FT ++    +  TV  +Y+ F   ++VG
Sbjct: 159 IAIMLDTKGPEVRSGD--VPQPIFLEEGQEFNFTIKRGVSLKD-TVSVNYDDFVNDVEVG 218

Query: 216 DSVVIDGGMATFEVIEKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSSKD 275
           D +++DGGM +  V  K  + + C   D G    R      R   V  ++   P+++ KD
Sbjct: 219 DILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSR------RHLNVRGKSATLPSITDKD 278

Query: 276 WSDIEFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLEEII 335
           W DI+FG+   V+F A+SFV  A  V  LKNYL T SA  + V+ KIES +S++NL  II
Sbjct: 279 WEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSA-DISVIVKIESADSIKNLPSII 338

Query: 336 EASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPTRAE 395
            A DG MVARGDLG E+P+E++P +QEEI   CR ++KPVI+A+ +LESM+ +PTPTRAE
Sbjct: 339 SACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAE 398

Query: 396 VADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHYQLG 455
           V+D++ AVR+ ADA+MLSGE+A G +  KA++V+   + R E         +    Y+  
Sbjct: 399 VSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTAYK-- 458

Query: 456 VSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRM 515
                 + +     AS +AN LS   + V T  G MA LLS  RP   IFA T+      
Sbjct: 459 ----GHMGQMFAFHASIMANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQ 518

Query: 516 ALNLQWGVFPLRIELSEDIEANIWRGIELVKSKGLVKQGDSVLVVSEISPARAASMASQS 575
            L L  GV P+ +E S+D E    R ++L++ + ++K+G  V +V   S       ++  
Sbjct: 519 RLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWREESTHL 567

Query: 576 IQLKTI 581
           IQ++ I
Sbjct: 579 IQVRKI 567

[Close]
BLAST of CmaCh16G003030 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 226.9 bits (577), Expect = 4.3e-59
Identity = 162/502 (32.27%), Postives = 261/502 (51.99%), Query Frame = 0 

Query: 97  KTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCIS 156
           KTK+VCT+GPA  ++E +EKL   GMN+AR N  H S  +H + +  ++   E      +
Sbjct: 17  KTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMENTCIPCA 76

Query: 157 VMIDTEGSQIHV--VDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVG 216
           VM+DT+G +I    +  G P  + ++   I + T    EG  S T+  SY+  +E ++ G
Sbjct: 77  VMLDTKGPEIRTGFLKEGKPVEL-IQGQEITISTDYTMEG-DSNTISMSYKKLAEDLKSG 136

Query: 217 DSVVIDGGMATFEVI--EKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSS 276
           D ++   G  +  V+  +K    +  +C +  +   R   +    G VV    + PTL+ 
Sbjct: 137 DVILCSDGTISLTVLSCDKNLGLVRARCENSAVLGERKNVNL--PGIVV----DLPTLTE 196

Query: 277 KDWSDI-EFGIAEGVNFIALSFV-NGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNL 336
           KD  DI ++G+   ++ IALSFV  G+D V+  K  L  ++AKS+ +++K+E+ E + N 
Sbjct: 197 KDQEDILQWGVPNKIDIIALSFVRKGSDLVEVRK--LLGENAKSIMLMSKVENQEGVMNF 256

Query: 337 EEIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTP 396
           ++I+E SD  MVARGDLG+EIP+E++   Q+ +      L KP++ A+Q+LESM + P P
Sbjct: 257 DKILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMTKSPRP 316

Query: 397 TRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPH 456
           TRAE  DV+ AV    D +MLSGE+A G++ + A  VL M+    E        E+F+ +
Sbjct: 317 TRAEATDVANAVLDGTDCVMLSGETAAGAHPETA--VLTMSRICKE-------AEDFIDY 376

Query: 457 YQLGVSLHDRIA------EEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIF 516
             +   + D ++      E +  SA   A  L   AI VLT  G+   L+++ RP  PI 
Sbjct: 377 DTMHKKIQDIVSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPIL 436

Query: 517 ALTDDDSTRMALNLQW-----------------GVFPL-------RIELSEDIEANIWRG 563
           ++   + TR   + +W                 G+ P+       R    +  E  I   
Sbjct: 437 SVIVPEITRTD-DFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFA 496

[Close]
BLAST of CmaCh16G003030 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 225.7 bits (574), Expect = 9.5e-59
Identity = 157/494 (31.78%), Postives = 254/494 (51.42%), Query Frame = 0 

Query: 97  KTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCIS 156
           KTK+VCT+GPA  ++  +EKL   GMN+AR N  H S E+H + +  ++   +  G   +
Sbjct: 21  KTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRTAMQNTGILAA 80

Query: 157 VMIDTEGSQIHV--VDHGAPSSIKVEDGSIWLFTAEKFEGYRSFTVQASYEGFSEGIQVG 216
           VM+DT+G +I    +  G P  +K E   I + T    +G    T+  SY+     ++ G
Sbjct: 81  VMLDTKGPEIRTGFLKDGNPIQLK-EGQEITITTDYDIKGDEK-TISMSYKKLPVDVKPG 140

Query: 217 DSVVIDGGMATFEVI--EKIGNDLSCKCTDPGLFLPRAKYSFWRDGRVVVRNHESPTLSS 276
           ++++   G  +  V+  +     + C+C +  +   R   +    G VV    + PTL+ 
Sbjct: 141 NTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL--PGVVV----DLPTLTD 200

Query: 277 KDWSDI-EFGIAEGVNFIALSFVNGADPVKHLKNYLSTKSAKSVRVLAKIESLESLQNLE 336
           KD  DI ++G+   ++ IALSFV     + +++  L + S KS+ +++K+E+ E + N +
Sbjct: 201 KDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHS-KSIMLMSKVENQEGVLNFD 260

Query: 337 EIIEASDGIMVARGDLGVEIPLEQIPAIQEEITSVCRELNKPVIIASQLLESMVEYPTPT 396
           EI+  +D  MVARGDLG+EIP+E+I   Q+ +   C    KPV+ A+Q+LESM++ P PT
Sbjct: 261 EILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPT 320

Query: 397 RAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASCRMELWSREENRENFLPHY 456
           RAE  DV+ AV    D +MLSGESA G+Y +  ++V  MA   +E  S  +    F    
Sbjct: 321 RAEATDVANAVLDGTDCVMLSGESAAGAYPE--IAVKTMAKICIEAESSLDYNTIFKEMI 380

Query: 457 QLGVSLHDRIAEEICNSASELANRLSVDAIFVLTTQGHMASLLSRNRPRPPIFAL----- 516
           +    L     E + +SA   AN+     I VLT  G  A L+++ RP  PI ++     
Sbjct: 381 R-ATPLPMSTLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVPVF 440

Query: 517 ---------TDDDSTRMALNLQWGVFPL------RIELSEDIEANIWRGIELVKSKGLVK 566
                    +D+   R +L +  G+ P+      +   SE  E  I   ++    KGL  
Sbjct: 441 TSDTFNWSCSDESPARHSL-IYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGLCN 500

The following BLAST results are available for this feature:
BLAST of CmaCh16G003030 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LIK01.1e-16859.96Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q431172.7e-16758.45Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q405453.1e-16357.43Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q405468.9e-8640.22Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW93.7e-8434.78Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
BLAST of CmaCh16G003030 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1J2T20.0e+00100.00Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111482878 PE=3 SV=1[more]
A0A6J1E4Y30.0e+0096.72Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430639 PE=3 SV=1[more]
A0A5A7TLC26.7e-30291.22Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350... [more]
A0A5D3E0J92.5e-30191.05Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0035... [more]
E5GBH72.5e-30191.05Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
BLAST of CmaCh16G003030 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022984667.10.0e+00100.00plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita maxima][more]
XP_023553283.10.0e+0097.76plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG7014927.10.0e+0097.24Plastidial pyruvate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyr... [more]
XP_022922736.10.0e+0096.72plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita moschata][more]
XP_038905899.18.6e-30492.41plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida][more]
BLAST of CmaCh16G003030 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G22960.17.8e-17059.96Pyruvate kinase family protein [more]
AT5G52920.12.7e-8534.78plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.12.9e-8439.09plastidial pyruvate kinase 3 [more]
AT3G04050.14.3e-5932.27Pyruvate kinase family protein [more]
AT5G63680.19.5e-5931.78Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 317..343
score: 52.48
coord: 369..393
score: 57.66
coord: 413..429
score: 35.11
coord: 344..368
score: 48.43
coord: 153..169
score: 27.42
coord: 394..412
score: 54.25
coord: 286..300
score: 40.72
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 96..567
e-value: 6.9E-121
score: 402.0
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 39..580
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 96..435
e-value: 1.1E-136
score: 458.1
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 463..563
e-value: 2.3E-20
score: 72.9
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 164..268
e-value: 1.1E-136
score: 458.1
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 95..433
e-value: 2.9E-85
score: 286.2
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 436..562
e-value: 1.1E-136
score: 458.1
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 459..562
NoneNo IPR availablePANTHERPTHR11817:SF21PYRUVATE KINASEcoord: 39..580
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 315..327
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 90..436
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 166..250

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G003030.1CmaCh16G003030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity