CmaCh15G009820 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh15G009820
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPectin lyase-like superfamily protein
LocationCma_Chr15: 5589077 .. 5595231 (+)
RNA-Seq ExpressionCmaCh15G009820
SyntenyCmaCh15G009820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCAGAATGGCCACGTGTGGAAGTTCTCCCATCTTATGGGAGGGGAAGAGATGCTCTGGGTACATCAATCTTATCTTTAGAACTAATCTCACGGATGTAGTTATCACAAGGTGATTATTTTTCTATTGCCTTTTAATATTCTAATTTTTTTTTTTTTTTAAAATGGTTTGTGTTAAGGATATTTGACCGTTCATTATAAGGCAAATATCTAGATATTTGCCTTACATTACCGGTTACCATATTTTCCCTTTTACTAAATGCAAATATATTGTATTCATTTATTATTCTTTCCTTTTATAGCTATTTCCTTATTATTTGTAATTTACCTTATTTCTGTATTCTGTAAATGATTATAAATAAGAGAACTCTCACCACCATTTGGGTGTGGTGGATTAATCAAACATTCACATGGTATCAGAGCCCTTTCGGTTTAACAACCCTGATCCTTTTTTCTCTATGGCTTCCGAATCTTGTTATCATCTTCTTCCCTTCAATACTCTAATCCATATGATCACCATCAAACTTTCTTCCTCCAATTATCTTCTTTGGAAAAGTCAACTTCTCCCTCTTCTTGAGAGTCAAGACATGCTGGGCTATGTCGATGGAACTATGGTTCCACCACCTCGCTTTGAACCAGAAACCTCCTCAACATTCAACCCCAAATATTTGGCATGGAGAGCAGCCGATCAACGACTTCTCTGTCTCCTGCTCTCCTCTCTCACTGAGGAAGCCATGGCTGTTGTCGTTGGTCTCTCTACTGCACGTGATGTTTGGCTTGCATTGGAAACTACGTTCAGCCATCAGTCAAAAGCTCGTGAACTGAGACTCAAGGATGACTTGCAGTTGATGAAACGTGGCACAAAACCTGTTGCTGAGTATGCCCGTGCCTTCAAAAAAATTTGTGACCAACTTCATGCCATTGGCAGACCCGTCGAGGACATTGATAAAGTGCACTGGTTCCTTCGTGGACTCGGCACCGAATTTTCAGCTTTTTCTACTGCTCAGATGGCTCTCACCCCTATCCCCTGTTTTGCAGATCTAGTCTCTAAAACTGAAAGTTTTGAGTTGTTCCAGCGCTCCCTTGAGTCCTCTGACTCCACTCCTACAGCATTCATAGCCACTAATCGTGGTCGCACCCATGAAAGTCACCCTGCTTCCTTTACCAACCAGCGAGGTCGTTCTTATTCTCAGAAAAACAACTCTTCTAATCGAGGACGAACCCACTCAAGTCAGGGTCGTCGACCACCTCATTGCCAAATATGCCGCAAAGAGGGCCATTATGCTGACCGCTGCAACCAACGGTATGTTCGACCTGATTCTTCTCATGCTCACCTTGCTGAAGCCTTTANCACGTCATGTTCTATTGCTGGACCCGATGCTGCTGATTGGTTTTTGGACACTGGAGCTTCGGCCCATATGACTGCCGACCCATCAATTCTGGATCAGTCTAAAAATTACACGGGTAAGGACTCTGTGATTGTAGGAAACGGTGCATCCCTACCCATTACCCACATCGGTACTCTTTCTTCTGTTCCAAATATTCACTTATTAGATGTCTTGGTTGTCCCTCACCTCACTAAAAATCTTCTTTCCTTCTGTTCCAAATATTCACTTATTAGATGTCTTGGTTGTCCCTCACCTCACTAAAAATCTTCTTTCCATAAGTAAATTAACGTCTGATTTTCCTCTCTCCGTTACATTTACTAATAATCTTATTACCATCCAGAATCATCAAACAGGAAGGGTGGTGGCAACCGGTAAAAGAGATTGAGGGCTATATGTGCTGGAGCCGGCAACTCTGCTTTTATTTCAGCCCTTAAAAACAAATCTTTACGTGCTTCATATGATTTATGGCATGCTCGTCTGGGTCATGTGAATCATTCTGTTATTTCTTTTTTAAATAAAAAAGGTCATCTTTCTCTTACGTCTTTATTGCCTTCTCCATCATTATGTAATACTTGTCAGCTTGCGAAAAGTCATCGATTGCCTTATTCCCGCAATGAACGTAGGTCGTCTCATGTGTTAGATCTTATTCATTGTGATCTTCGGGGTCCTTCTTCCGTCAAATCAAATTCGGGTTTTCTTTATTATGCTATTTTTATTGATGATTATTCTCGATTCACTTGGTTTTACCCTTTAAAATTTAAATCTGATTTTTTTGATATTTTTCTTCAATTTCAAAAATTTGTGGAAAATCAATATTCTTCTCGTATCAAGGTATTTCAAAGCGATGGTGGTACCGAACTTACTAGTACTTGTTTCAAAACTCATTTACGTAATTCTGGCATCCATCATCAACTCTCTTGTCCATATACACCTGCTCAAAATGGTCGTGTTGAGAGAAAACATCGTCATGTGACTGAGACTGGCTTGGCCCTTCTCGTTCACTCTCATCTTTCTCCTCGTTTTTGGGTTGACGCCTTCAGCACTGCAGCTTATATTATCAACCGGTTGCCTACTCCACTTCTTGGAGGTAAGTCACCCTTTGAACTCCTTTATGGCTACACTCCACATTATGACAATTTTCATCCCTTTGGTTGTCGTGTTTATCCTTATTTGCGTGATTATATGCCTAACAAGCTTTCTCCCCGCAGCATTCCTTGTATTTTTTTGGGTTATAGTCCTGTTCATAAAGGGTTCCTCTGTCTTGATCCCGCCACCACTAAGCTATATATCACCTGCCATGCTCAATTTGATGAAACTCACTTTCCTGCTATCCCTAGCTCCCAAGCCCAACCTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCTGTGCAGGTTGATACTTCTCTTGCAGGTTCCACTTTGCCACCCTCGACTTCTAATTCAACCTCTATTGAACCTCCTGTTGATTTCTCTTCTTTGGGCACTCATCCTATGATCACACGCGCCAAATCTGGTATATTCAAGACTCGTCATCCAGCAAATCTTGGTATGTTGGGCTCATCTGGACTTCTCTCTGCTCTTCTTGCATCCACTGAGCCAAAAGGATTCAANAGGATTCAAATCTGCGGCTAAGAATCCTGCTTGGGTGGCTGCCATGGATGAAGAAATTCGAGCGTTATAACAAAATGATACTTGGACTTTGGTTCCTCGCCCTGCCAATACCAACATCGTGGGCTCTAAATGGGTGTTTCGTATTAAATATTTGCCCGATGGATCCGTCGAGCGTTTCAAGGCTCGTCTCGTTGCCAAAGGTTATACTCAGGTTCCTGGTCTTGACTACACTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTCTTGAAGCTTCACCCACTTCTGATGGTCTCTTTATCAGTCAGTTAAAATATGCTCGAGATATTCTTACTCGTGCTCAGTTGCTCGATAGCAAACCAGTTCACACTCCCATGGTTGTTTCTCAACACCTGACTGCTGATGGTTCTCCTTTCTCTGATCCTACTCTCTACAGATCTCTTGTTGGCGCCCTTCAGTACTTGACTATCACGCGTCCAGATATTGCCTATGCTGTCAATTCTGTCAGTCAATTCTTGCATGCCCCTACTGCAAATCACTTTCTTGCTGTCAAACGTATTCTTCGCTATGTTAAAGGAACACTCCACTTTGGTCTTATCTTTCGTCCATCCACTGTTCCTAGTACGCTAGTCGCTTATTCGGATGCTGACTGGGCTGGTTGTCCCGATACTCGTCGTTCTACATCCGACTATTCTATTTATCTTGGTAACAATCTCATTTCTTGGAGTACCAAAAAGCAACCTACTGTATCACGCTCCAGCTGTGAATCTGAGTATCGTGCTCTTGCCACAACTGCTGCTAAACTTCTTTGGGTTACGCATCTTTTGCATGACCTCAAGGTCCCTATTTCACAGCAGCCCTTACTCTTATGTGACAACAAAAGTGCTATTTTTTTGAGCTCTATCCTGTTTCTCACAAGCGGGCCAAGCATGTTGAACTAGATTATCATTTCCTTCGAGAACTAGTTATCGCTGGCAAACTTCGTACACAATATGTACCCTCTCATCTCCAAGTTGCTGACATCTTCACAAAGAGTATTTCTCGACCTCTCTTTGAATTTTTCAGATCCAAGCTTCATGTTCGTTCAAATCCGACGCTCAGCTTGCGAGGGGGTGTTAAGGATATTTGACCGTTCATTATAAGGCAAATATCTAGATATTTGCCTTACATTACCGGTTACCATATTTTCCCTTTTACTAAATGCAAATATATTGTATTCATTTATTATTCTTTCCTTTCATCGCTATTTCCTTATTATTTGTAATTTACCTTATTTCTGTATTCTGTAAATGATTATAAATAAGAGAACTCTCACCACCATTTGGGTGTGGTGGATTAATCAAACATTCACAGTTTGCAAAATTATAATCTTTTATAGGCTTAAATGCTTCAAAATTAAATAAAAAAAATTATGGTAGATGGAAATATAAAAAAAGGGTTGATGACATAGTGTAGGTAACCACGGTACAATCGACGGGCAGAGATCTTCTTGGTGGGAAAAATTTAAGAAAGGGGAGCTCAAGGTAACACGACCATATGTGATCGAGATCATGTACTCTTGATCAAATTCAAATTTCGGATCTCACTTTGATCAACTCCCCTTCTTAGTTTGTCCATCTGATATATTGCAGGTTTCACTATTTCACCTCAATCCCATTATACTTGTATTTTAGGATTATGATTATGCTGCCATTCAAATCATTCCCAACTAAAAGCAAATTTGATTTATATATATATATATATATATATTATGATCATTTTTTAATTAGTTTGGTTTGGTTCACTGAAAATGCAGAAATGTTATTATCCAAGGGTTTACTATCTTGGCTCCCGTCACCGTACCCAACACCGACGGAATCAATCCAGGTTCCTTCTTCTTCACCTTTAAAACCTTAGGGTTCTTTTCATTGTCAGTCCCTCTCCCTAATTCTCTATTCAATTTATAAGATTCTTGTTCCAACACTCGAATCGAAGATTGCTACATTGTCTCAGGTGATGATTGCATTGCCATCAAGAGCGGATGGGACCAATATGGTATCAAGTTTGGAATGCCGACCGAGGACCTTGTCATTCGCCGCCTCACTTGCATCTCACCAGATTTTGCAGGTATTGCCCTTGGCAGCGAAATGTCTGGCGGAATTCGCAACGTCAGGATCAAAAACGTCACTGCAATCAATACACAGTCTACTATCAAAATCAAAACCGCTCGCAGATGA

mRNA sequence

ATGAATCAGAATGGCCACGTGTGGAAGTTCTCCCATCTTATGGGAGGGGAAGAGATGCTCTGGGTAACCACGGTACAATCGACGGGCAGAGATCTTCTTGGTGGGAAAAATTTAAGAAAGGGGAGCTCAAGAAATGTTATTATCCAAGGGTTTACTATCTTGGCTCCCGTCACCGTACCCAACACCGACGGAATCAATCCAGATTCTTGTTCCAACACTCGAATCGAAGATTGCTACATTGTCTCAGGTGATGATTGCATTGCCATCAAGAGCGGATGGGACCAATATGGTATCAAGTTTGGAATGCCGACCGAGGACCTTGTCATTCGCCGCCTCACTTGCATCTCACCAGATTTTGCAGGTATTGCCCTTGGCAGCGAAATGTCTGGCGGAATTCGCAACGTCAGGATCAAAAACGTCACTGCAATCAATACACAGTCTACTATCAAAATCAAAACCGCTCGCAGATGA

Coding sequence (CDS)

ATGAATCAGAATGGCCACGTGTGGAAGTTCTCCCATCTTATGGGAGGGGAAGAGATGCTCTGGGTAACCACGGTACAATCGACGGGCAGAGATCTTCTTGGTGGGAAAAATTTAAGAAAGGGGAGCTCAAGAAATGTTATTATCCAAGGGTTTACTATCTTGGCTCCCGTCACCGTACCCAACACCGACGGAATCAATCCAGATTCTTGTTCCAACACTCGAATCGAAGATTGCTACATTGTCTCAGGTGATGATTGCATTGCCATCAAGAGCGGATGGGACCAATATGGTATCAAGTTTGGAATGCCGACCGAGGACCTTGTCATTCGCCGCCTCACTTGCATCTCACCAGATTTTGCAGGTATTGCCCTTGGCAGCGAAATGTCTGGCGGAATTCGCAACGTCAGGATCAAAAACGTCACTGCAATCAATACACAGTCTACTATCAAAATCAAAACCGCTCGCAGATGA

Protein sequence

MNQNGHVWKFSHLMGGEEMLWVTTVQSTGRDLLGGKNLRKGSSRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGMPTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTARR
Homology
BLAST of CmaCh15G009820 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.2e-46
Identity = 89/138 (64.49%), Postives = 111/138 (80.43%), Query Frame = 0

Query: 18  EMLWVTTVQSTGRDLLGGK--NLRKGSSRNVIIQGFTILAPVTVPNTDGINPDSCSNTRI 77
           E+++   +Q +   LL     N+    SRN++IQG TILAPV  PNTDGINPDSC+NTRI
Sbjct: 203 ELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNTRI 262

Query: 78  EDCYIVSGDDCIAIKSGWDQYGIKFGMPTEDLVIRRLTCISPDFAGIALGSEMSGGIRNV 137
           EDCYIVSGDDC+A+KSGWD+YGI +GMPT+ LVIRRLTCISP  A IALGSEMSGGI++V
Sbjct: 263 EDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQDV 322

Query: 138 RIKNVTAINTQSTIKIKT 154
           R +++ AIN++S I+IKT
Sbjct: 323 RAEDIVAINSESGIRIKT 340

BLAST of CmaCh15G009820 vs. ExPASy Swiss-Prot
Match: P27644 (Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.7e-13
Identity = 43/112 (38.39%), Postives = 59/112 (52.68%), Query Frame = 0

Query: 52  TILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGMPTED----- 111
           TI+AP   PNTDG NP+SC N  I       GDDCIA+K+G      K G   ED     
Sbjct: 62  TIIAPHDSPNTDGFNPESCRNVMISGVRFSVGDDCIAVKAG------KRGPDGEDDHLAE 121

Query: 112 --LVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTARR 157
              +  R   + P   G+ +GSEMSGG+ +V +++   I T   +++KT  R
Sbjct: 122 TRGITVRHCLMQPGHGGLVIGSEMSGGVHDVTVEDCDMIGTDRGLRLKTGAR 167

BLAST of CmaCh15G009820 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.5e-08
Identity = 42/112 (37.50%), Postives = 57/112 (50.89%), Query Frame = 0

Query: 46  VIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGMPTE 105
           V I    I AP + PNTDGI+  + SN  I+DC I +GDDCIAI SG           T 
Sbjct: 172 VTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TS 231

Query: 106 DLVIRRLTCISPDFAGIALGSEMSGG----IRNVRIKNVTAINTQSTIKIKT 154
           ++ I  + C  P   GI++GS    G    + NV ++N     T +  +IKT
Sbjct: 232 NIHISGIDC-GPGH-GISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKT 270

BLAST of CmaCh15G009820 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 4.5e-08
Identity = 41/117 (35.04%), Postives = 60/117 (51.28%), Query Frame = 0

Query: 41  GSSRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKF 100
           G    V IQG  I AP   PNTDGI+  +     IE C I +GDDC+A+ +G        
Sbjct: 213 GECDGVKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG-------- 272

Query: 101 GMPTEDLVIRRLTCISPDFAGIALGSEMSGGIRN----VRIKNVTAINTQSTIKIKT 154
              + ++ I+ LTC  P   G+++GS   G  R+    V +     I+TQ+ ++IKT
Sbjct: 273 ---SSNITIKDLTC-GPGH-GMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKT 316

BLAST of CmaCh15G009820 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.8e-08
Identity = 41/117 (35.04%), Postives = 58/117 (49.57%), Query Frame = 0

Query: 41  GSSRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKF 100
           G    V IQG  I AP   PNTDGI+  +     IE C I +GDDCIAI +G        
Sbjct: 212 GECEGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG-------- 271

Query: 101 GMPTEDLVIRRLTCISPDFAGIALGS----EMSGGIRNVRIKNVTAINTQSTIKIKT 154
              + ++ I+ L C  P   GI++GS         + +V +     I+TQ+ ++IKT
Sbjct: 272 ---SSNITIKDLIC-GPGH-GISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKT 315

BLAST of CmaCh15G009820 vs. ExPASy TrEMBL
Match: A0A6J1E3E2 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111430236 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.9e-54
Identity = 107/113 (94.69%), Postives = 110/113 (97.35%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVIIQG TILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM
Sbjct: 223 SRNVIIQGLTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 282

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTEDLVIRRLTCISPD AGIALGSEMSGGIRNVRI+NVTAINTQS ++IKTAR
Sbjct: 283 PTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAR 335

BLAST of CmaCh15G009820 vs. ExPASy TrEMBL
Match: A0A6J1E7Y4 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111430234 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 9.5e-54
Identity = 106/113 (93.81%), Postives = 109/113 (96.46%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVIIQG TILAPVTVPNTDGINPDSCSN RIEDCYIVSGDDCIAIKSGWDQYGIKFGM
Sbjct: 340 SRNVIIQGLTILAPVTVPNTDGINPDSCSNIRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 399

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTEDLVIRRLTCISPD AGIALGSEMSGGIRNVRI+NVTAINTQS ++IKTAR
Sbjct: 400 PTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAR 452

BLAST of CmaCh15G009820 vs. ExPASy TrEMBL
Match: A0A6J1E8J0 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111430374 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.6e-53
Identity = 103/113 (91.15%), Postives = 109/113 (96.46%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVIIQG TILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIA+KSGWDQYGIKFGM
Sbjct: 189 SRNVIIQGLTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAVKSGWDQYGIKFGM 248

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTEDL+IRRLTCISPD A IALGSEMSGGIRNVRI+NVTAINTQ+ ++IKTAR
Sbjct: 249 PTEDLIIRRLTCISPDSAAIALGSEMSGGIRNVRIENVTAINTQAAVRIKTAR 301

BLAST of CmaCh15G009820 vs. ExPASy TrEMBL
Match: A0A6J1E7Z5 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111430246 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.6e-53
Identity = 105/113 (92.92%), Postives = 108/113 (95.58%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVII G TILAPVTVPNTDGINPDSCSNTR EDCYIVSGDDCIAIKSGWDQYGIKFGM
Sbjct: 222 SRNVIILGLTILAPVTVPNTDGINPDSCSNTRFEDCYIVSGDDCIAIKSGWDQYGIKFGM 281

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTEDLVIRRLTCISPD AGIALGSEMSGGIRNVRI+NVTAINTQS ++IKTAR
Sbjct: 282 PTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAR 334

BLAST of CmaCh15G009820 vs. ExPASy TrEMBL
Match: A0A6J1I677 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111471479 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.1e-52
Identity = 105/113 (92.92%), Postives = 108/113 (95.58%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVIIQG TILAPVTVPNTDGINPDSCSN RIEDCYIVSGDDCIAIKSGWDQYGIKFGM
Sbjct: 256 SRNVIIQGLTILAPVTVPNTDGINPDSCSNIRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 315

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTE LVIRRLTCISPD AGIALGSEMSGGIRNVRI+NVTAINTQS ++IKTAR
Sbjct: 316 PTEYLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAR 368

BLAST of CmaCh15G009820 vs. NCBI nr
Match: XP_023521226.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 221.9 bits (564), Expect = 4.0e-54
Identity = 107/113 (94.69%), Postives = 110/113 (97.35%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVIIQG TILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM
Sbjct: 222 SRNVIIQGLTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 281

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTEDLVIRRLTCISPD AGIALGSEMSGGIRNVRI+NVTAINTQS ++IKTAR
Sbjct: 282 PTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAR 334

BLAST of CmaCh15G009820 vs. NCBI nr
Match: XP_022922171.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 221.9 bits (564), Expect = 4.0e-54
Identity = 107/113 (94.69%), Postives = 110/113 (97.35%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVIIQG TILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM
Sbjct: 223 SRNVIIQGLTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 282

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTEDLVIRRLTCISPD AGIALGSEMSGGIRNVRI+NVTAINTQS ++IKTAR
Sbjct: 283 PTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAR 335

BLAST of CmaCh15G009820 vs. NCBI nr
Match: XP_022922170.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 219.5 bits (558), Expect = 2.0e-53
Identity = 106/113 (93.81%), Postives = 109/113 (96.46%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVIIQG TILAPVTVPNTDGINPDSCSN RIEDCYIVSGDDCIAIKSGWDQYGIKFGM
Sbjct: 340 SRNVIIQGLTILAPVTVPNTDGINPDSCSNIRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 399

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTEDLVIRRLTCISPD AGIALGSEMSGGIRNVRI+NVTAINTQS ++IKTAR
Sbjct: 400 PTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAR 452

BLAST of CmaCh15G009820 vs. NCBI nr
Match: XP_023520463.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 219.5 bits (558), Expect = 2.0e-53
Identity = 106/113 (93.81%), Postives = 109/113 (96.46%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVIIQG TILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM
Sbjct: 222 SRNVIIQGLTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 281

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTEDLVIRRLTCISPD AGIALGSEMSGGIRNVRI+NV AINTQS ++IKTAR
Sbjct: 282 PTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVIAINTQSAVRIKTAR 334

BLAST of CmaCh15G009820 vs. NCBI nr
Match: XP_023550020.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 218.0 bits (554), Expect = 5.7e-53
Identity = 105/113 (92.92%), Postives = 109/113 (96.46%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           SRNVII+G TILAPVTVPNTDGINPDSCSN RIEDCYIVSGDDCIAIKSGWDQYGIKFGM
Sbjct: 222 SRNVIIRGLTILAPVTVPNTDGINPDSCSNIRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 281

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTAR 156
           PTEDLVIRRLTCISPD AGIALGSEMSGGIRNVRI+NVTAINTQS ++IKTAR
Sbjct: 282 PTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAR 334

BLAST of CmaCh15G009820 vs. TAIR 10
Match: AT4G23500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 189.5 bits (480), Expect = 2.0e-48
Identity = 88/112 (78.57%), Postives = 101/112 (90.18%), Query Frame = 0

Query: 43  SRNVIIQGFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGM 102
           S N+ IQG TILAPVTVPNTDGINPDSC+NTRIEDCYIVSGDDCIA+KSGWDQYGI +GM
Sbjct: 235 SSNIYIQGLTILAPVTVPNTDGINPDSCTNTRIEDCYIVSGDDCIAVKSGWDQYGINYGM 294

Query: 103 PTEDLVIRRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTA 155
           PT+ L+IRRLTCISPD A IALGSEMSGGI +VR +++ AIN++S I+IKTA
Sbjct: 295 PTKQLLIRRLTCISPDSAVIALGSEMSGGIEDVRAEDIVAINSESGIRIKTA 346

BLAST of CmaCh15G009820 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 189.1 bits (479), Expect = 2.7e-48
Identity = 91/139 (65.47%), Postives = 111/139 (79.86%), Query Frame = 0

Query: 18  EMLWVTTVQSTGRDLLGGK--NLRKGSSRNVIIQGFTILAPVTVPNTDGINPDSCSNTRI 77
           E+L+   +Q +   L+     N+      +VI++  T+LAPVTVPNTDGINPDSC+NT I
Sbjct: 186 EILFSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAPVTVPNTDGINPDSCTNTLI 245

Query: 78  EDCYIVSGDDCIAIKSGWDQYGIKFGMPTEDLVIRRLTCISPDFAGIALGSEMSGGIRNV 137
           EDCYIVSGDDCIA+KSGWDQYGIKFGMPT+ L IRRLTCISP  AG+ALGSEMSGGI++V
Sbjct: 246 EDCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCISPKSAGVALGSEMSGGIKDV 305

Query: 138 RIKNVTAINTQSTIKIKTA 155
           RI++VT  NT+S I+IKTA
Sbjct: 306 RIEDVTLTNTESAIRIKTA 324

BLAST of CmaCh15G009820 vs. TAIR 10
Match: AT2G23900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 186.8 bits (473), Expect = 1.3e-47
Identity = 93/165 (56.36%), Postives = 117/165 (70.91%), Query Frame = 0

Query: 1   MNQNGHVWKFSHLMGG---------EEMLWVTTVQSTGRDLLGGK--NLRKGSSRNVIIQ 60
           +N  G  W   +  GG          E+++   VQ +   ++     N+      NVI++
Sbjct: 171 INGQGKYWWVKYRSGGFKGITRPYTIEIIFSQNVQISNITIIDSPAWNIHPVYCNNVIVK 230

Query: 61  GFTILAPVTVPNTDGINPDSCSNTRIEDCYIVSGDDCIAIKSGWDQYGIKFGMPTEDLVI 120
           G TILAP+  PNTDGINPDSC+NT IEDCY+VSGDDCIA+KSGWDQ+GIK GMPT+ L I
Sbjct: 231 GVTILAPIDSPNTDGINPDSCTNTLIEDCYVVSGDDCIAVKSGWDQFGIKVGMPTQQLSI 290

Query: 121 RRLTCISPDFAGIALGSEMSGGIRNVRIKNVTAINTQSTIKIKTA 155
           RRLTCISPD AGIALGSEMSGGI++VRI+++T + TQS I+IKTA
Sbjct: 291 RRLTCISPDSAGIALGSEMSGGIKDVRIEDITLLQTQSAIRIKTA 335

BLAST of CmaCh15G009820 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 8.5e-47
Identity = 87/139 (62.59%), Postives = 109/139 (78.42%), Query Frame = 0

Query: 18  EMLWVTTVQSTGRDLLGGK--NLRKGSSRNVIIQGFTILAPVTVPNTDGINPDSCSNTRI 77
           E+++  T+Q +    L     N+    S N+I++G TI+APV  PNTDGINPDSC+NTRI
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRI 244

Query: 78  EDCYIVSGDDCIAIKSGWDQYGIKFGMPTEDLVIRRLTCISPDFAGIALGSEMSGGIRNV 137
           EDCYI+SGDDCIA+KSGWD+YGI FGMPT+ LVIRRLTCISP  A IALGSEMSGGI +V
Sbjct: 245 EDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDV 304

Query: 138 RIKNVTAINTQSTIKIKTA 155
           R +++TA  T+S ++IKTA
Sbjct: 305 RAEDITAYQTESGVRIKTA 323

BLAST of CmaCh15G009820 vs. TAIR 10
Match: AT3G61490.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 8.5e-47
Identity = 87/139 (62.59%), Postives = 109/139 (78.42%), Query Frame = 0

Query: 18  EMLWVTTVQSTGRDLLGGK--NLRKGSSRNVIIQGFTILAPVTVPNTDGINPDSCSNTRI 77
           E+++  T+Q +    L     N+    S N+I++G TI+APV  PNTDGINPDSC+NTRI
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRI 244

Query: 78  EDCYIVSGDDCIAIKSGWDQYGIKFGMPTEDLVIRRLTCISPDFAGIALGSEMSGGIRNV 137
           EDCYI+SGDDCIA+KSGWD+YGI FGMPT+ LVIRRLTCISP  A IALGSEMSGGI +V
Sbjct: 245 EDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDV 304

Query: 138 RIKNVTAINTQSTIKIKTA 155
           R +++TA  T+S ++IKTA
Sbjct: 305 RAEDITAYQTESGVRIKTA 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL33.2e-4664.49Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
P276442.7e-1338.39Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1[more]
Q9LW071.5e-0837.50Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q9FY194.5e-0835.04Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Q7M1E75.8e-0835.04Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E3E21.9e-5494.69probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111430236 PE=3 SV... [more]
A0A6J1E7Y49.5e-5493.81probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111430234 PE=3 SV... [more]
A0A6J1E8J03.6e-5391.15probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111430374 PE=3 SV... [more]
A0A6J1E7Z53.6e-5392.92probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111430246 PE=3 SV... [more]
A0A6J1I6771.1e-5292.92probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111471479 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023521226.14.0e-5494.69probable polygalacturonase [Cucurbita pepo subsp. pepo][more]
XP_022922171.14.0e-5494.69probable polygalacturonase [Cucurbita moschata][more]
XP_022922170.12.0e-5393.81probable polygalacturonase [Cucurbita moschata][more]
XP_023520463.12.0e-5393.81probable polygalacturonase [Cucurbita pepo subsp. pepo][more]
XP_023550020.15.7e-5392.92probable polygalacturonase [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G23500.12.0e-4878.57Pectin lyase-like superfamily protein [more]
AT3G48950.12.7e-4865.47Pectin lyase-like superfamily protein [more]
AT2G23900.11.3e-4756.36Pectin lyase-like superfamily protein [more]
AT3G61490.18.5e-4762.59Pectin lyase-like superfamily protein [more]
AT3G61490.28.5e-4762.59Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 43..69
e-value: 1600.0
score: 5.8
coord: 132..153
e-value: 1600.0
score: 5.8
coord: 104..126
e-value: 2100.0
score: 5.0
coord: 70..91
e-value: 8.6
score: 15.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 34..156
e-value: 2.7E-39
score: 137.2
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 42..154
e-value: 4.4E-17
score: 62.1
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 43..154
NoneNo IPR availablePANTHERPTHR31339:SF12ENDO-POLYGALACTURONASE-LIKE PROTEINcoord: 43..154
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 42..154

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G009820.1CmaCh15G009820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity