CmaCh15G009670 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh15G009670
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectinesterase
Location	Cma_Chr15: 5369571 .. 5370491 (-)
RNA-Seq Expression	CmaCh15G009670
Synteny	CmaCh15G009670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGCGTTGTTTTCTTCTCTTTTCATTCCTAGGGATCACCCATGCCCAAAGCTTCCACACTTTCATGTCTTGCTCCCAAACCCCATATCCAGACCTCTGCTTCCATTTCATCAACCCGAATCACAACATACCCACCACCGACGTCCATCAATCTTATCACATCACTCGCTTTCGAAACTCGGCCATTCGAGCCACTTTGAACCAAGCCATGGTGGCCCATCACCACCTTCTCTCTGCCATGGATTTAGCTTCCTTTGACGATGACTACAGAGTGAAATCAGCTTTAATCGACTGTCTGGAACTCTACGAAGATTCAATCACCGAGCTCAATCGCTCGATGATGAACTCTATGGGGGCCCATTCAATTGACCACTTGACATTGCTAAGTGCTTCATTAGCGAATCATCAAACATGCCGAGATGGGTTTCGAGATTTTGGATTTTCTTCTCATTATTTGAATATCATTTTTCCGCATCAAATTTTGAGTAATTTCTCGAAATTGATCAGCAATTCTCTTGCCATCACCAAGATCGCTGCCGTGACAGCTACTACTTCTTCCACCCCTTATAGCCAGCGGATACTAGCTGGTTCGTTTCCCAAGTGGGTTTCTGGTGATGATAGGATGCTCCTCCGGGACACGACCGTGAAGCGCGCGGATATGGTGGTGGCCCAAGATGGGAGCGGCAATTTCAGAACGATATCGGAGGCGGTGGCCGCAGCGGCGGAGGTTAGGAAGGCAAGTGAAAGAAGGTTTGTTATATATGTAAAAGGTGGGATTTACAGAGAGAATGTTGTGATTAAGAAAACGTTGAAGAATGTTATGATGATTGGAGATGGGATGGGTGCGACGATCGTTACAGCCATGAAAAACGTCAAAGATGGATCAACCACTTTCAGATCAGCCACTTTTGGTTCGTAA

mRNA sequence

ATGCTGCGTTGTTTTCTTCTCTTTTCATTCCTAGGGATCACCCATGCCCAAAGCTTCCACACTTTCATGTCTTGCTCCCAAACCCCATATCCAGACCTCTGCTTCCATTTCATCAACCCGAATCACAACATACCCACCACCGACGTCCATCAATCTTATCACATCACTCGCTTTCGAAACTCGGCCATTCGAGCCACTTTGAACCAAGCCATGGTGGCCCATCACCACCTTCTCTCTGCCATGGATTTAGCTTCCTTTGACGATGACTACAGAGTGAAATCAGCTTTAATCGACTGTCTGGAACTCTACGAAGATTCAATCACCGAGCTCAATCGCTCGATGATGAACTCTATGGGGGCCCATTCAATTGACCACTTGACATTGCTAAGTGCTTCATTAGCGAATCATCAAACATGCCGAGATGGGTTTCGAGATTTTGGATTTTCTTCTCATTATTTGAATATCATTTTTCCGCATCAAATTTTGAGTAATTTCTCGAAATTGATCAGCAATTCTCTTGCCATCACCAAGATCGCTGCCGTGACAGCTACTACTTCTTCCACCCCTTATAGCCAGCGGATACTAGCTGGTTCGTTTCCCAAGTGGGTTTCTGGTGATGATAGGATGCTCCTCCGGGACACGACCGTGAAGCGCGCGGATATGGTGGTGGCCCAAGATGGGAGCGGCAATTTCAGAACGATATCGGAGGCGGTGGCCGCAGCGGCGGAGGTTAGGAAGGCAAGTGAAAGAAGGTTTGTTATATATGTAAAAGGTGGGATTTACAGAGAGAATGTTGTGATTAAGAAAACGTTGAAGAATGTTATGATGATTGGAGATGGGATGGGTGCGACGATCGTTACAGCCATGAAAAACGTCAAAGATGGATCAACCACTTTCAGATCAGCCACTTTTGGTTCGTAA

Coding sequence (CDS)

ATGCTGCGTTGTTTTCTTCTCTTTTCATTCCTAGGGATCACCCATGCCCAAAGCTTCCACACTTTCATGTCTTGCTCCCAAACCCCATATCCAGACCTCTGCTTCCATTTCATCAACCCGAATCACAACATACCCACCACCGACGTCCATCAATCTTATCACATCACTCGCTTTCGAAACTCGGCCATTCGAGCCACTTTGAACCAAGCCATGGTGGCCCATCACCACCTTCTCTCTGCCATGGATTTAGCTTCCTTTGACGATGACTACAGAGTGAAATCAGCTTTAATCGACTGTCTGGAACTCTACGAAGATTCAATCACCGAGCTCAATCGCTCGATGATGAACTCTATGGGGGCCCATTCAATTGACCACTTGACATTGCTAAGTGCTTCATTAGCGAATCATCAAACATGCCGAGATGGGTTTCGAGATTTTGGATTTTCTTCTCATTATTTGAATATCATTTTTCCGCATCAAATTTTGAGTAATTTCTCGAAATTGATCAGCAATTCTCTTGCCATCACCAAGATCGCTGCCGTGACAGCTACTACTTCTTCCACCCCTTATAGCCAGCGGATACTAGCTGGTTCGTTTCCCAAGTGGGTTTCTGGTGATGATAGGATGCTCCTCCGGGACACGACCGTGAAGCGCGCGGATATGGTGGTGGCCCAAGATGGGAGCGGCAATTTCAGAACGATATCGGAGGCGGTGGCCGCAGCGGCGGAGGTTAGGAAGGCAAGTGAAAGAAGGTTTGTTATATATGTAAAAGGTGGGATTTACAGAGAGAATGTTGTGATTAAGAAAACGTTGAAGAATGTTATGATGATTGGAGATGGGATGGGTGCGACGATCGTTACAGCCATGAAAAACGTCAAAGATGGATCAACCACTTTCAGATCAGCCACTTTTGGTTCGTAA

Protein sequence

MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAHSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAAVTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSATFGS

Homology

[Close]

BLAST of CmaCh15G009670 vs. ExPASy Swiss-Prot
Match: O49298 (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PME6 PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 6.8e-53
Identity = 143/327 (43.73%), Postives = 194/327 (59.33%), Query Frame = 0


Query: 3   RCFLLFSF-LGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNS 62
           +C +   + + I+H  + H   SC QTPYP +C H ++   N P   +        F + 
Sbjct: 16  KCIITIIYVVSISHLNA-HFITSCKQTPYPSVCDHHMS---NSPLKTLDDQTDGFTFHDL 75

Query: 63  AIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAH 122
            + +T++QA+  H  + S     S        SAL DCLELYED+I +LN S   S G +
Sbjct: 76  VVSSTMDQAVQLHRLVSSLKQHHSLHK--HATSALFDCLELYEDTIDQLNHS-RRSYGQY 135

Query: 123 SIDH--LTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIA 182
           S  H   T LSA++AN  TCR+GFRDF  +S Y +  FP Q   N +K ISNSLA+TK A
Sbjct: 136 SSPHDRQTSLSAAIANQDTCRNGFRDFKLTSSY-SKYFPVQFHRNLTKSISNSLAVTKAA 195

Query: 183 AVT-------ATTSSTPYS-QRILAG-------------SFPKWVSGDDRMLLRDT-TVK 242
           A          +T  T +S QR  AG              FP W    DR LL D+ T  
Sbjct: 196 AEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTA 255

Query: 243 RADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMI 302
           +AD+VVA+DGSG++ +I +AV AAA++ + ++ R VIYVK G+YRENVVIKK++KNVM+I
Sbjct: 256 KADLVVAKDGSGHYTSIQQAVNAAAKLPRRNQ-RLVIYVKAGVYRENVVIKKSIKNVMVI 315

Query: 303 GDGMGATIVTAMKNVKDGSTTFRSATF 305
           GDG+ +TIVT  +NV+DG+TTFRSATF
Sbjct: 316 GDGIDSTIVTGNRNVQDGTTTFRSATF 333

[Close]

BLAST of CmaCh15G009670 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 5.1e-40
Identity = 110/279 (39.43%), Postives = 157/279 (56.27%), Query Frame = 0


Query: 25  CSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMDLA 84
           C +TP P  C +F+        TDV      T F   +++  L +A  A     +   L 
Sbjct: 32  CGKTPNPQPCEYFLTQK-----TDVTSIKQDTDFYKISLQLALERATTAQSRTYT---LG 91

Query: 85  SFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAHSIDHLTLLSASLANHQTCRDGFR 144
           S   + R K+A  DC ELYE ++ +LN++  +S G   +D  T LS +L N +TCR    
Sbjct: 92  SKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLE 151

Query: 145 DFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAAVTATTSSTPYSQRILAGSFPKWVS 204
           D G   + L +     + +N +KLISN+L++ K+          PY++      FP WV 
Sbjct: 152 DLGVPEYVLPL-----LSNNVTKLISNTLSLNKV----------PYNEPSYKDGFPTWVK 211

Query: 205 GDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVKGGIYREN 264
             DR LL+  T  RA++VVAQDGSGN +TI EAVAAA+   +A   R+VIY+K G Y EN
Sbjct: 212 PGDRKLLQ--TTPRANIVVAQDGSGNVKTIQEAVAAAS---RAGGSRYVIYIKAGTYNEN 271

Query: 265 VVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSAT 304
           + +K  LKN+M +GDG+G TI+T  K+V  G+TTF+SAT
Sbjct: 272 IEVK--LKNIMFVGDGIGKTIITGSKSVGGGATTFKSAT 280

[Close]

BLAST of CmaCh15G009670 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 4.5e-36
Identity = 109/279 (39.07%), Postives = 159/279 (56.99%), Query Frame = 0


Query: 25  CSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMDLA 84
           CSQTP P  C +F+  N N            + F   +++  L++A++A  H   A  L 
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSE-----SEFLKISMKLVLDRAILAKTH---AFTLG 93

Query: 85  SFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAHSIDHLTLLSASLANHQTCRDGFR 144
               D R K+A  DC++LY+ +++++N +M  ++    +D  T LS +L N  TCR GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 145 DFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAAVTATTSSTPYSQRILAGSFPKWVS 204
           + G +    +I+ P  + +N S L+ N+LAI K+       + TP  +      FP WV 
Sbjct: 154 ELGVT----DIVLP-LMSNNVSNLLCNTLAINKV-----PFNYTPPEK----DGFPSWVK 213

Query: 205 GDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVKGGIYREN 264
             DR LL+ +T K  + VVA+DGSGNF+TI EA+ AA     +   RFVIYVK G+Y EN
Sbjct: 214 PGDRKLLQSSTPK-DNAVVAKDGSGNFKTIKEAIDAA-----SGSGRFVIYVKQGVYSEN 273

Query: 265 VVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSAT 304
           + I+K  KNVM+ GDG+G TI+T  K+V  G+TTF SAT
Sbjct: 274 LEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSAT 282

[Close]

BLAST of CmaCh15G009670 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.4e-34
Identity = 101/288 (35.07%), Postives = 155/288 (53.82%), Query Frame = 0


Query: 25  CSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMDLA 84
           C +TPYPD C  +   NHN       Q   ++ FR   + A +++A+ A   L ++    
Sbjct: 38  CDKTPYPDPCKCYFK-NHN----GFQQPTQLSEFRVMLVEAAMDRAISARAELTNSGKNC 97

Query: 85  SFDDDYRVKSALIDCLELYEDSITELNRSMMN-------SMGAHSIDHLTLLSASLANHQ 144
           +   D + ++ L DC++LY D+I +LNR++         +      D  T LS +L N +
Sbjct: 98  T---DSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTE 157

Query: 145 TCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAAVTATTSSTPYSQRILAG 204
           TCR G  D   +    + I P    +  S LISN LA+           +T  +Q+    
Sbjct: 158 TCRRGSSDLNVT----DFITPIVSNTKISHLISNCLAVNGALLTAGNKGNTTANQK---- 217

Query: 205 SFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVK 264
            FP W+S  D+ LLR     RA++VVA+DGSG+F T+  A+  A   RK +  RFVIYVK
Sbjct: 218 GFPTWLSRKDKRLLR---AVRANLVVAKDGSGHFNTVQAAIDVAGR-RKVTSGRFVIYVK 277

Query: 265 GGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSATFG 306
            GIY+EN+ ++    ++M++GDGM +TI+T  ++V+ G TT+ SAT G
Sbjct: 278 RGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAG 305

[Close]

BLAST of CmaCh15G009670 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.2e-33
Identity = 108/301 (35.88%), Postives = 155/301 (51.50%), Query Frame = 0


Query: 24  SCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHH------- 83
           SCS T YPDLCF  I          V ++      +   I  +LN    A  H       
Sbjct: 72  SCSSTRYPDLCFSAI--------AAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQK 131

Query: 84  LLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSM--------MNSMGAHSIDHLTL 143
           LL   +L       R K AL DCLE  ++++ EL++++          S+  H+ D  TL
Sbjct: 132 LLKRTNLTK-----REKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTL 191

Query: 144 LSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAAVTATTSST 203
           +SA++ N  TC DGF     + H  + +   Q+  +  K+ SN+LA+ K    T      
Sbjct: 192 MSAAMTNQGTCLDGFSHDDANKHVRDALSDGQV--HVEKMCSNALAMIKNMTDTDMMIMR 251

Query: 204 PYSQRIL------AGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAA 263
             + R L         +P W+S  DR LL+ ++V   + VVA DGSGNF+T++ AVAAA 
Sbjct: 252 TSNNRKLTEETSTVDGWPAWLSPGDRRLLQSSSV-TPNAVVAADGSGNFKTVAAAVAAAP 311

Query: 264 EVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSA 304
           +      +R++I +K G+YRENV + K  KN+M IGDG   TI+T  +NV DGSTTF+SA
Sbjct: 312 Q---GGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSA 353

[Close]

BLAST of CmaCh15G009670 vs. ExPASy TrEMBL
Match: A0A6J1IBL7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472075 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 4.8e-166
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60
           MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN
Sbjct: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60

Query: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120
           SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA
Sbjct: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120

Query: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAA 180
           HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAA
Sbjct: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAA 180

Query: 181 VTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAA 240
           VTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAA
Sbjct: 181 VTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAA 240

Query: 241 AAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFR 300
           AAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFR
Sbjct: 241 AAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFR 300

Query: 301 SATF 305
           SATF
Sbjct: 301 SATF 304

[Close]

BLAST of CmaCh15G009670 vs. ExPASy TrEMBL
Match: A0A6J1FKP5 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111445093 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.5e-153
Identity = 286/305 (93.77%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60
           ML CFLLFSFLGITHAQSFH FMSCSQTPYPDLCFHFI PNHN+PTTDVHQSYHITRFRN
Sbjct: 3   MLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRN 62

Query: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120
           SAIRATLNQAMVAHHHLLS+MDLASF DD RVKSALIDCLELYEDSITELNRS+MNSM A
Sbjct: 63  SAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKA 122

Query: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYL-NIIFPHQILSNFSKLISNSLAITKIA 180
           +SI HLTLLSASLANHQTCRDGFRDFGFSSHYL NIIFPHQILSNFSKLISNSLAITK A
Sbjct: 123 NSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAA 182

Query: 181 AVTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVA 240
           AVTATTSSTP SQR+LAGSFPKWVSGDDRMLLRDTT KR DMVVAQDGSGNFRTISEAVA
Sbjct: 183 AVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVA 242

Query: 241 AAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTF 300
           AAAEVRK SERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMG TIVTAMKNVKDGSTTF
Sbjct: 243 AAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTF 302

Query: 301 RSATF 305
           RSATF
Sbjct: 303 RSATF 307

[Close]

BLAST of CmaCh15G009670 vs. ExPASy TrEMBL
Match: A0A1S3CSS2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103504549 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.6e-105
Identity = 210/302 (69.54%), Postives = 238/302 (78.81%), Query Frame = 0


Query: 4   CFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAI 63
           CFLLFS L I H +SF+ FMSC QTPYPDLCFH+INPN NIPT D+ ++Y  TRFRNSA+
Sbjct: 6   CFLLFSLLVIIHGRSFNNFMSCFQTPYPDLCFHYINPNDNIPTADIDETYLNTRFRNSAV 65

Query: 64  RATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAHSI 123
           +ATLNQA   H HL S MDL SFDDD R K ALIDCLE YEDSI +LNRS+ +S   +SI
Sbjct: 66  QATLNQARKVHDHLRSIMDLGSFDDDNRNKVALIDCLEFYEDSIAKLNRSVFSSTSVNSI 125

Query: 124 DHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAAVTA 183
           DHLT+LSASLANHQTC DGFRDF  S+   N   P QI+SNFSKL+SNSLAITK A   A
Sbjct: 126 DHLTMLSASLANHQTCLDGFRDFDNSN---NFFLPIQIMSNFSKLVSNSLAITKAAVAPA 185

Query: 184 TTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAE 243
           ++SST   Q +  G  P+WV  DD+ LLRD   K ADMVVAQDGSG+FRTISEAVAAA E
Sbjct: 186 SSSSTSCGQPLPDGLCPEWVYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISEAVAAAEE 245

Query: 244 VRKAS-ERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSA 303
            RK S   RFVIYVKGGIY+ENVVIKK++KNVMMIGDGM  TI+TAMKNV+DGSTTF+SA
Sbjct: 246 ARKGSGSERFVIYVKGGIYKENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDGSTTFQSA 304

Query: 304 TF 305
           TF
Sbjct: 306 TF 304

[Close]

BLAST of CmaCh15G009670 vs. ExPASy TrEMBL
Match: A0A0A0KUU2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G568300 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.6e-103
Identity = 212/307 (69.06%), Postives = 241/307 (78.50%), Query Frame = 0


Query: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60
           +L CFLLFS L I H +SF+ FMSCSQTPYPDLCFH+INPN NI T  + ++Y ITRFRN
Sbjct: 3   ILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRN 62

Query: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120
           SAI+ATL QAM    HL S MDL SFDD+ R   ALIDCLE YEDSI ELNRS ++S  A
Sbjct: 63  SAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSSTSA 122

Query: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIF-PHQILSNFSKLISNSLAITK-I 180
           +SIDH T+LSASLANHQTC DGFRDFGF     N  F P QI+SNFSKL+SNSLAITK I
Sbjct: 123 NSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAI 182

Query: 181 AAVTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAV 240
           AA  +T+SST   Q +L G  P+WV  DD+ LL+D   K ADMVVAQDGSG+FRTISEAV
Sbjct: 183 AAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAV 242

Query: 241 AAAAEVRKAS-ERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGST 300
           AAA E RK S   RFVIYVKGGIY+ENVVI+K +KN+MM+GDGM  TIVTAMKNV+DGST
Sbjct: 243 AAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGST 302

Query: 301 TFRSATF 305
           TF+SATF
Sbjct: 303 TFQSATF 309

[Close]

BLAST of CmaCh15G009670 vs. ExPASy TrEMBL
Match: A0A5D3E4L0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00430 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 1.3e-99
Identity = 200/283 (70.67%), Postives = 225/283 (79.51%), Query Frame = 0


Query: 23  MSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMD 82
           MSCSQTPYPDLCFH+INPN NIPT D+ ++Y  TRFRNSA++ATLNQA   H HL S MD
Sbjct: 1   MSCSQTPYPDLCFHYINPNDNIPTADIDETYLNTRFRNSAVQATLNQARKVHDHLRSIMD 60

Query: 83  LASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAHSIDHLTLLSASLANHQTCRDG 142
           L SFDDD R K ALIDCLE YEDSI +LNRS  +S   +SIDHLT+LSASLANHQTC DG
Sbjct: 61  LGSFDDDNRNKVALIDCLEFYEDSIAKLNRSAFSSTIVNSIDHLTMLSASLANHQTCLDG 120

Query: 143 FRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAAVTATTSSTPYSQRILAGSFPKW 202
           FRDF  S+   N   P QI+SNFSKL+SNSLAITK A   A++SST   Q +L G  P+W
Sbjct: 121 FRDFDNSN---NFFLPIQIMSNFSKLVSNSLAITKAAVAPASSSSTSCGQPLLDGLCPEW 180

Query: 203 VSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKAS-ERRFVIYVKGGIY 262
           V  DD+ LLRD   K ADMVVAQDGSG+FRTISEAVAAA E RK S   RFVIYVKGGIY
Sbjct: 181 VYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISEAVAAAEEARKGSGSERFVIYVKGGIY 240

Query: 263 RENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSATF 305
           +ENVVIKK++KNVMMIGDGM  TI+TAMKNV+DGSTTF+SATF
Sbjct: 241 KENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDGSTTFQSATF 280

[Close]

BLAST of CmaCh15G009670 vs. NCBI nr
Match: XP_022973540.1 (pectinesterase [Cucurbita maxima])

HSP 1 Score: 593.6 bits (1529), Expect = 9.8e-166
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60
           MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN
Sbjct: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60

Query: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120
           SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA
Sbjct: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120

Query: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAA 180
           HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAA
Sbjct: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAA 180

Query: 181 VTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAA 240
           VTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAA
Sbjct: 181 VTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAA 240

Query: 241 AAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFR 300
           AAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFR
Sbjct: 241 AAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFR 300

Query: 301 SATF 305
           SATF
Sbjct: 301 SATF 304

[Close]

BLAST of CmaCh15G009670 vs. NCBI nr
Match: XP_023549991.1 (pectinesterase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 553.5 bits (1425), Expect = 1.1e-153
Identity = 287/305 (94.10%), Postives = 293/305 (96.07%), Query Frame = 0


Query: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60
           ML CFLLFSFLGITHAQSFH FMSCSQTPYPDLCFHFINPNHN+PTTDVHQSY+ITRFRN
Sbjct: 3   MLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFINPNHNVPTTDVHQSYNITRFRN 62

Query: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120
           SAIRATLNQAMVAHHHLLSAMDLASFDDD RVKSALIDCLELYEDSITELNRS+MNSM A
Sbjct: 63  SAIRATLNQAMVAHHHLLSAMDLASFDDDDRVKSALIDCLELYEDSITELNRSVMNSMRA 122

Query: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYL-NIIFPHQILSNFSKLISNSLAITKIA 180
           +SIDHLTLLSASLANHQTCRDGFRDFGFSSHYL NIIFPHQILSNFSKLI+NSLAITK A
Sbjct: 123 NSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAITKAA 182

Query: 181 AVTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVA 240
           AVTATTSS P SQR+LAGSFPKWVS DDRMLLRDTT KRADMVVAQDGSGNFRTISEAVA
Sbjct: 183 AVTATTSSNPRSQRLLAGSFPKWVSSDDRMLLRDTTEKRADMVVAQDGSGNFRTISEAVA 242

Query: 241 AAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTF 300
           AAAEVRK SERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMG TIVTAMKNVKDGSTTF
Sbjct: 243 AAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTF 302

Query: 301 RSATF 305
           RSATF
Sbjct: 303 RSATF 307

[Close]

BLAST of CmaCh15G009670 vs. NCBI nr
Match: XP_022939088.1 (pectinesterase [Cucurbita moschata])

HSP 1 Score: 550.8 bits (1418), Expect = 7.3e-153
Identity = 286/305 (93.77%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60
           ML CFLLFSFLGITHAQSFH FMSCSQTPYPDLCFHFI PNHN+PTTDVHQSYHITRFRN
Sbjct: 3   MLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRN 62

Query: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120
           SAIRATLNQAMVAHHHLLS+MDLASF DD RVKSALIDCLELYEDSITELNRS+MNSM A
Sbjct: 63  SAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKA 122

Query: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYL-NIIFPHQILSNFSKLISNSLAITKIA 180
           +SI HLTLLSASLANHQTCRDGFRDFGFSSHYL NIIFPHQILSNFSKLISNSLAITK A
Sbjct: 123 NSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAA 182

Query: 181 AVTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVA 240
           AVTATTSSTP SQR+LAGSFPKWVSGDDRMLLRDTT KR DMVVAQDGSGNFRTISEAVA
Sbjct: 183 AVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVA 242

Query: 241 AAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTF 300
           AAAEVRK SERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMG TIVTAMKNVKDGSTTF
Sbjct: 243 AAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTF 302

Query: 301 RSATF 305
           RSATF
Sbjct: 303 RSATF 307

[Close]

BLAST of CmaCh15G009670 vs. NCBI nr
Match: KAG6579261.1 (putative pectinesterase/pectinesterase inhibitor 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 515.0 bits (1325), Expect = 4.4e-142
Identity = 268/285 (94.04%), Postives = 273/285 (95.79%), Query Frame = 0


Query: 23  MSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMD 82
           MSCSQTPYPDLCFHFI PNHN+PTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLS+MD
Sbjct: 1   MSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMD 60

Query: 83  LASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAHSIDHLTLLSASLANHQTCRDG 142
           LASF DD RVKSALIDCLELYEDSITELNRS+MNSM A+SI HLTLLSASLANHQTCRDG
Sbjct: 61  LASFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKANSIGHLTLLSASLANHQTCRDG 120

Query: 143 FRDFGFSSHYL-NIIFPHQILSNFSKLISNSLAITKIAAVTATTSSTPYSQRILAGSFPK 202
           FRDFGFSSHYL NIIFPHQILSNFSKLISNSLAITK AAVTATTSSTP SQR+LAGSFPK
Sbjct: 121 FRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAAVTATTSSTPGSQRLLAGSFPK 180

Query: 203 WVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVKGGIY 262
           WVSGDDRMLLRDTT KR DMVVAQDGSGNFRTISEAVAAAAEVRK SERRFVIYVKGGIY
Sbjct: 181 WVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRFVIYVKGGIY 240

Query: 263 RENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSATFGS 307
           RENVVIKKTLKNVMMIGDGMG TIVTAMKNVKDGSTTFRSATFGS
Sbjct: 241 RENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFGS 285

[Close]

BLAST of CmaCh15G009670 vs. NCBI nr
Match: KAG7016777.1 (putative pectinesterase/pectinesterase inhibitor 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 514.6 bits (1324), Expect = 5.8e-142
Identity = 268/285 (94.04%), Postives = 273/285 (95.79%), Query Frame = 0


Query: 23  MSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMD 82
           MSCSQTPYPDLCFHFI PNHN+PTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLS+MD
Sbjct: 1   MSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMD 60

Query: 83  LASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAHSIDHLTLLSASLANHQTCRDG 142
           LASF DD RVKSALIDCLELYEDSITELNRS+MNSM A+SI HLTLLSASLANHQTCRDG
Sbjct: 61  LASFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKANSIAHLTLLSASLANHQTCRDG 120

Query: 143 FRDFGFSSHYL-NIIFPHQILSNFSKLISNSLAITKIAAVTATTSSTPYSQRILAGSFPK 202
           FRDFGFSSHYL NIIFPHQILSNFSKLISNSLAITK AAVTATTSSTP SQR+LAGSFPK
Sbjct: 121 FRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAAVTATTSSTPGSQRLLAGSFPK 180

Query: 203 WVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVKGGIY 262
           WVSGDDRMLLRDTT KR DMVVAQDGSGNFRTISEAVAAAAEVRK SERRFVIYVKGGIY
Sbjct: 181 WVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRFVIYVKGGIY 240

Query: 263 RENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSATFGS 307
           RENVVIKKTLKNVMMIGDGMG TIVTAMKNVKDGSTTFRSATFGS
Sbjct: 241 RENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFGS 285

[Close]

BLAST of CmaCh15G009670 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 209.1 bits (531), Expect = 4.9e-54
Identity = 143/327 (43.73%), Postives = 194/327 (59.33%), Query Frame = 0


Query: 3   RCFLLFSF-LGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNS 62
           +C +   + + I+H  + H   SC QTPYP +C H ++   N P   +        F + 
Sbjct: 16  KCIITIIYVVSISHLNA-HFITSCKQTPYPSVCDHHMS---NSPLKTLDDQTDGFTFHDL 75

Query: 63  AIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAH 122
            + +T++QA+  H  + S     S        SAL DCLELYED+I +LN S   S G +
Sbjct: 76  VVSSTMDQAVQLHRLVSSLKQHHSLHK--HATSALFDCLELYEDTIDQLNHS-RRSYGQY 135

Query: 123 SIDH--LTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIA 182
           S  H   T LSA++AN  TCR+GFRDF  +S Y +  FP Q   N +K ISNSLA+TK A
Sbjct: 136 SSPHDRQTSLSAAIANQDTCRNGFRDFKLTSSY-SKYFPVQFHRNLTKSISNSLAVTKAA 195

Query: 183 AVT-------ATTSSTPYS-QRILAG-------------SFPKWVSGDDRMLLRDT-TVK 242
           A          +T  T +S QR  AG              FP W    DR LL D+ T  
Sbjct: 196 AEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTA 255

Query: 243 RADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMI 302
           +AD+VVA+DGSG++ +I +AV AAA++ + ++ R VIYVK G+YRENVVIKK++KNVM+I
Sbjct: 256 KADLVVAKDGSGHYTSIQQAVNAAAKLPRRNQ-RLVIYVKAGVYRENVVIKKSIKNVMVI 315

Query: 303 GDGMGATIVTAMKNVKDGSTTFRSATF 305
           GDG+ +TIVT  +NV+DG+TTFRSATF
Sbjct: 316 GDGIDSTIVTGNRNVQDGTTTFRSATF 333

[Close]

BLAST of CmaCh15G009670 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 153.3 bits (386), Expect = 3.2e-37
Identity = 109/279 (39.07%), Postives = 159/279 (56.99%), Query Frame = 0


Query: 25  CSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMDLA 84
           CSQTP P  C +F+  N N            + F   +++  L++A++A  H   A  L 
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSE-----SEFLKISMKLVLDRAILAKTH---AFTLG 93

Query: 85  SFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGAHSIDHLTLLSASLANHQTCRDGFR 144
               D R K+A  DC++LY+ +++++N +M  ++    +D  T LS +L N  TCR GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 145 DFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAAVTATTSSTPYSQRILAGSFPKWVS 204
           + G +    +I+ P  + +N S L+ N+LAI K+       + TP  +      FP WV 
Sbjct: 154 ELGVT----DIVLP-LMSNNVSNLLCNTLAINKV-----PFNYTPPEK----DGFPSWVK 213

Query: 205 GDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVKGGIYREN 264
             DR LL+ +T K  + VVA+DGSGNF+TI EA+ AA     +   RFVIYVK G+Y EN
Sbjct: 214 PGDRKLLQSSTPK-DNAVVAKDGSGNFKTIKEAIDAA-----SGSGRFVIYVKQGVYSEN 273

Query: 265 VVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSAT 304
           + I+K  KNVM+ GDG+G TI+T  K+V  G+TTF SAT
Sbjct: 274 LEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSAT 282

[Close]

BLAST of CmaCh15G009670 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 147.5 bits (371), Expect = 1.7e-35
Identity = 101/288 (35.07%), Postives = 155/288 (53.82%), Query Frame = 0


Query: 25  CSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMDLA 84
           C +TPYPD C  +   NHN       Q   ++ FR   + A +++A+ A   L ++    
Sbjct: 38  CDKTPYPDPCKCYFK-NHN----GFQQPTQLSEFRVMLVEAAMDRAISARAELTNSGKNC 97

Query: 85  SFDDDYRVKSALIDCLELYEDSITELNRSMMN-------SMGAHSIDHLTLLSASLANHQ 144
           +   D + ++ L DC++LY D+I +LNR++         +      D  T LS +L N +
Sbjct: 98  T---DSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTE 157

Query: 145 TCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITKIAAVTATTSSTPYSQRILAG 204
           TCR G  D   +    + I P    +  S LISN LA+           +T  +Q+    
Sbjct: 158 TCRRGSSDLNVT----DFITPIVSNTKISHLISNCLAVNGALLTAGNKGNTTANQK---- 217

Query: 205 SFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIYVK 264
            FP W+S  D+ LLR     RA++VVA+DGSG+F T+  A+  A   RK +  RFVIYVK
Sbjct: 218 GFPTWLSRKDKRLLR---AVRANLVVAKDGSGHFNTVQAAIDVAGR-RKVTSGRFVIYVK 277

Query: 265 GGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSATFG 306
            GIY+EN+ ++    ++M++GDGM +TI+T  ++V+ G TT+ SAT G
Sbjct: 278 RGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAG 305

[Close]

BLAST of CmaCh15G009670 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 145.2 bits (365), Expect = 8.6e-35
Identity = 104/290 (35.86%), Postives = 154/290 (53.10%), Query Frame = 0


Query: 25  CSQTPYPDLCFHFI--NPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSAMD 84
           C +TPYP  C  +   +    +PT        I+ FR   + A +++A+ A   L ++  
Sbjct: 42  CDKTPYPYPCKRYFIKHSGFRLPT-------QISEFRVLLVEAAMDRAVSAWDKLTNSSK 101

Query: 85  LASFDDDYRVKSALIDCLELYEDSITELNRSMM-----NSMGAHSIDHLTLLSASLANHQ 144
             +   D++ ++ L DC+ LY D++ +LNR++              D  T LS +L N +
Sbjct: 102 NCT---DFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRRCTDFDAQTWLSTALTNTE 161

Query: 145 TCRDGFRDFGFSSHYLNIIFPHQILSN--FSKLISNSLAITKIAAVTATTSSTPYSQRIL 204
           TCR G  D   S       F   I+SN   S LISN LA+           ST    +  
Sbjct: 162 TCRRGSSDLNVSD------FTTPIVSNTKISHLISNCLAVNGALLTAGKNDSTTGDSK-- 221

Query: 205 AGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVAAAAEVRKASERRFVIY 264
              FP WVS  +R LL+  +V RA++VVA+DGSG+F+T+  A+  A   RK +  RFVIY
Sbjct: 222 --GFPTWVSRKERRLLQLQSV-RANLVVAKDGSGHFKTVQAAIDVAGR-RKVTSGRFVIY 281

Query: 265 VKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTFRSATFG 306
           VK GIY+EN+ ++    N+M++GDGM  TI+T  ++VK G TT+ SAT G
Sbjct: 282 VKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAG 309

[Close]

BLAST of CmaCh15G009670 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 135.2 bits (339), Expect = 8.9e-32
Identity = 105/307 (34.20%), Postives = 154/307 (50.16%), Query Frame = 0


Query: 25  CSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRNSAIRATLNQAMVA-HHHLLSAMDL 84
           CS T YP+LCF  +        T           +   I A+LN    A  H+  +   L
Sbjct: 66  CSSTLYPELCFSAVAATGGKELTS----------QKEVIEASLNLTTKAVKHNYFAVKKL 125

Query: 85  ASFDDDY--RVKSALIDCLELYEDSITELNRSM--------MNSMGAHSIDHLTLLSASL 144
            +       R  +AL DCLE  ++++ EL+ ++          S+  H+ D  TL+S+++
Sbjct: 126 IAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAI 185

Query: 145 ANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAI------TKIAAVTATTSS 204
            N  TC DGF            +   Q+  +   + SN+LA+      T IA       S
Sbjct: 186 TNQGTCLDGFSYDDADRKVRKALLKGQV--HVEHMCSNALAMIKNMTETDIANFELRDKS 245

Query: 205 TPYSQR-----------ILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISE 264
           + ++             + +  +PKW+S  DR LL+ +T+K AD  VA DGSG+F T++ 
Sbjct: 246 STFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGSTIK-ADATVADDGSGDFTTVAA 305

Query: 265 AVAAAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGS 304
           AVAAA E    S +RFVI++K G+YRENV + K   N+M +GDG G TI+T  +NV DGS
Sbjct: 306 AVAAAPE---KSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 356

The following BLAST results are available for this feature:

BLAST of CmaCh15G009670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49298	6.8e-53	43.73	Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=37...	[more]
O04887	5.1e-40	39.43	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	4.5e-36	39.07	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN5	2.4e-34	35.07	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
O04886	1.2e-33	35.88	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]

BLAST of CmaCh15G009670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IBL7	4.8e-166	100.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472075 PE=3 SV=1	[more]
A0A6J1FKP5	3.5e-153	93.77	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111445093 PE=3 SV=1	[more]
A0A1S3CSS2	1.6e-105	69.54	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103504549 PE=3 SV=1	[more]
A0A0A0KUU2	2.6e-103	69.06	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G568300 PE=3 SV=1	[more]
A0A5D3E4L0	1.3e-99	70.67	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00430...	[more]

BLAST of CmaCh15G009670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022973540.1	9.8e-166	100.00	pectinesterase [Cucurbita maxima]	[more]
XP_023549991.1	1.1e-153	94.10	pectinesterase [Cucurbita pepo subsp. pepo]	[more]
XP_022939088.1	7.3e-153	93.77	pectinesterase [Cucurbita moschata]	[more]
KAG6579261.1	4.4e-142	94.04	putative pectinesterase/pectinesterase inhibitor 6, partial [Cucurbita argyrospe...	[more]
KAG7016777.1	5.8e-142	94.04	putative pectinesterase/pectinesterase inhibitor 6, partial [Cucurbita argyrospe...	[more]

BLAST of CmaCh15G009670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23200.1	4.9e-54	43.73	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G45220.1	3.2e-37	39.07	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	1.7e-35	35.07	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	8.6e-35	35.86	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G53830.1	8.9e-32	34.20	pectin methylesterase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 14..175 e-value: 1.6E-23 score: 94.1
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 23..177 e-value: 6.8E-17 score: 59.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 24..175 e-value: 1.9E-22 score: 80.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 23..179 e-value: 1.1E-24 score: 89.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 24..178
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 215..306 e-value: 7.1E-25 score: 90.0
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 220..305 e-value: 1.4E-26 score: 92.9
None	No IPR available	PANTHER	PTHR31707:SF152	PECTINESTERASE INHIBITOR 5-RELATED	coord: 19..304
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 19..304
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..25 score: 5.0
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 24..177 e-value: 2.81929E-28 score: 104.828
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 248..267
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 215..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh15G009670.1	CmaCh15G009670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity