CmaCh14G016650 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G016650
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionProtein-serine/threonine phosphatase
LocationCma_Chr14: 12453529 .. 12456698 (-)
RNA-Seq ExpressionCmaCh14G016650
SyntenyCmaCh14G016650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAATCTTAGAAGAGGTTTTCTTGCAAGAAACTTGTTCTTGCACGAATTTGGTTCTTGGAAGAGGTTTCTATGACACTGAGGCATCAAGGATGAATGTCGGCGACGCAGAATAAAGTTCTCGGGCCTTTGATTTGCGATTTCACCATCGGCATTGTAATCCATCGGTAGCCATAGTTTATTTTTCGATTTGTTTGCATTAGAATCCATGGGTTGTGTTCTTGGAAAGTGTTTAACTTCCAGTCGCTACCCGCGGCCTTCTGACGGCGATATGACCCAATCCTGCGATGCTCCGCCGTGTAGTTTAGAAGGAAAACATGTACTAACGGGAAGGTTATTGGAGTCTGTTCGTGTGCCTTCTCATAACTTCCGTTTGGAGTACTCGCTCCTGACCCAGCGCGGCTACTACCCTGATTCCCCTGATAAAGAAAACCAAGACAGCTTCTGTGTCAGTACACAAATGGGAGGTAATCCGAATATCCATTTATTTGGTGTCTTTGATGGCCATGGTCAATTCGGATCGCTGTGTTCTAATTTCGTTAAGGAGAAGATTGTGCAAATGCTATGTAACGATTCTAAATTGTTGGAAGATCCTGTTAAAGCTTATAATTCGGCATTTTTGACCTTAAATGATGAATTGCGTAGTAGTGATATTGATGATTCCATGAGCGGCACTACAGCCATTACTGCTCTTGTTCTTGGGAATACCCTTTACGTTGCCAATGTGGGCGATTCGAGGGCAGTAATGGCTGTTAGAGTTGGGAATCGAATTGTTGCCGAGGATATGTCCCATGATCAAACACCATTTAGGAAGGATGAGTGCGAGAGAGTAAAGCTGGCCGGGGCTAGAGTGTTGACCATTGATCAAATTGAAGGGCTTAAAGATCCGGATATTCAAGTATGGGGTGATGAGGAGACCGAGGGAAATGATCCTCCTCGATTGTGGTTTGCTGATAGCTTGTACCCTGGTACTGCATTTACACGGAGCGTAGGAGACAGTACAGCGGAGAAGATTGGTGTCACTGCTGTCCCTGAGGTTTCAGTAGTTGAGCTGACTCAAAACCATCTTTTCTTTGTTGTTGCAAGCGATGGAGTTTTCGAGTTCCTTACGAGCCAAGCTGTTGTTGATATGGTACAGTGCCTATCTGAAATCTTGAATGGACTTCACATTTTAGTATGAACTTTCTGATTTCAAATCAGTTCCTTGCCAATTATTCTTTAACAAAATCTTCACTTAGGCTGCAAGGTATACCGATCCACGGGATGCATGCTCAGCTATTGCTGGAGAATCATATAAGCTATGGCTGGATCATGAAAACCGGACAGATGATATAACAATCATCATCGTTCACATCAAGGGCTTCTCTAGTGTATGAAATTCTGTCCATATCCGTTCCATCGCTCTTATTTTTAGTCATTTGTTTCTTTTGCAATTGTGGAGCTTGTTGAACGTCTGTTGTTAGCCTCTGTACTAGTGAGGGGGATATGTTACTGAATATTGTGCGTGATTGGGTTTCAAGTGCTGAATTGTCCAATGGCCAATAATTCAAGACTGCGAAAAGTTGGTTTCGAGTTATGACACTGTTTCATGTAGTACTAGTCATGTTGCCCTCTTCTTTGGTACTTTAAAGAGGGCCTAAATTAGCCAAGTGAAATTTTCTTATCCTTTTGGTTTAAGCTTCATCCTATTGCTGTAATTAGGTACTCTTCCATGCAATGCTGTTCATTTTTTCCTCTTCTTTGGTATTTACGAGGACTAAAACTAACTATGTCAAAGTTTATTATCCTTTTGGTTTAAACCTCATCCTAATTGTTGTAATCAACTACGCTATATCCTTTTGGTTTCCCTTTTCTTTGGTACTTGTGAAGATTGAAATTTTCTTATCTTTTTGGTTTAGAGTATTAATCACATTTCCCTCTTTTTTTTTTTTTGGTACTTATGAGGACTAAAATAGCTCATCCTTTGGGTCTAAACTTCATTTTAATTGTTGTTGTTAGGTACATGTTCATATTGTTCTATTCATGTTCCTCTTCTTGGTACTAGGACTAAAGTTGTGTGAAAATTTCTTATCTTTTTTGTTTAAGCTTCATCTTAATTGTTGCAATCACGTAATTTTCCATGCAGTACTAATCATGTTTCCCTCCTTTTTAGTACCTGTGAAGATTAGAACTACTTCTGTGATTTTTTTTTTCTTTTTGGTTTAAACTTCATGTGAATTGCTATAGTCGAATCCTGTTCCATATAGTTTTAATCATGTTTCCCTCTCTGGTATTTATGAGGGCTAAAATTAGCTATATAAAATTTTCTTCTTCATCCTAATTGTTGTAGTAAGGTACTTTCCATATAGTATTAATCATGTTTTCCTCTTCATCCTAATTGTTATATACAAATTTCTTATTGGTTTTAACTTTATCCTAATTGTTGCAGTCAGGCGCTGCTGCAATTGTTGGGCAGCATGGACTCCGCATAAGCCATGCAAGTTCAGAGTGGGGAAGCTCGGAGGCCTTTTGCTCCATTAGAAGTGATTTCTCAGATCCACAGGTCTCTCAGCATGTGGGCTTGATGAACAGGAACACAGCAATTGCTGTTCCATCTCTCACGCAGTACCGACCATTGGAAGTGGTGAGTATTTTGATCTCTTTTTAGTATAAAAGCTTTTAAAACCATTCCATACCTTCTTGTCAAGTTGTTCAAGCTTAACAACCTGTCATGTTTCTTGCAGTTGTCTTTTATATCTCTTGTAGTGTATTTCTTGGAAACTTCATATAATATTATTGTGTTTGGTAACAGGAAGTGGGTTAATGGGGAGTATGCCTTCCCCTAGCTGCTAAAAAATATTCTCGTTTCGCTTGTAACAAAGATATCTTATCGTCAATGTTGCTAGTCAGTCCTAACCACAAGAATATGTATGTACAAGGAAGAACATATTGCTGTCTCTCTATCTGAGAGTTGCTCCAAGCTAAAGCCAACGAAATTATTGTACACTTGGAAATGGTGGCAACGATGATAAATCGACGGTGTTTCTCATTGAATGGAATTCAGAGAGAAGGAAATCGACATGATGGTTTGGTTTGGTTTCTTAGATTCCTCATATTCCCTGGGTGGTTTATGAAGGCCTTTTCCTGAACTTTGTATATATGATGATATTGTACAGAGATTCTTGACCGC

mRNA sequence

ATGAGAATCTTAGAAGAGGTTTTCTTGCAAGAAACTTGTTCTTGCACGAATTTGGTTCTTGGAAGAGAATCCATGGGTTGTGTTCTTGGAAAGTGTTTAACTTCCAGTCGCTACCCGCGGCCTTCTGACGGCGATATGACCCAATCCTGCGATGCTCCGCCGTGTAGTTTAGAAGGAAAACATGTACTAACGGGAAGGTTATTGGAGTCTGTTCGTGTGCCTTCTCATAACTTCCGTTTGGAGTACTCGCTCCTGACCCAGCGCGGCTACTACCCTGATTCCCCTGATAAAGAAAACCAAGACAGCTTCTGTGTCAGTACACAAATGGGAGGTAATCCGAATATCCATTTATTTGGTGTCTTTGATGGCCATGGTCAATTCGGATCGCTGTGTTCTAATTTCGTTAAGGAGAAGATTGTGCAAATGCTATGTAACGATTCTAAATTGTTGGAAGATCCTGTTAAAGCTTATAATTCGGCATTTTTGACCTTAAATGATGAATTGCGTAGTAGTGATATTGATGATTCCATGAGCGGCACTACAGCCATTACTGCTCTTGTTCTTGGGAATACCCTTTACGTTGCCAATGTGGGCGATTCGAGGGCAGTAATGGCTGTTAGAGTTGGGAATCGAATTGTTGCCGAGGATATGTCCCATGATCAAACACCATTTAGGAAGGATGAGTGCGAGAGAGTAAAGCTGGCCGGGGCTAGAGTGTTGACCATTGATCAAATTGAAGGGCTTAAAGATCCGGATATTCAAGTATGGGGTGATGAGGAGACCGAGGGAAATGATCCTCCTCGATTGTGGTTTGCTGATAGCTTGTACCCTGGTACTGCATTTACACGGAGCGTAGGAGACAGTACAGCGGAGAAGATTGGTGTCACTGCTGTCCCTGAGGTTTCAGTAGTTGAGCTGACTCAAAACCATCTTTTCTTTGTTGTTGCAAGCGATGGAGTTTTCGAGTTCCTTACGAGCCAAGCTGTTGTTGATATGGCTGCAAGGTATACCGATCCACGGGATGCATGCTCAGCTATTGCTGGAGAATCATATAAGCTATGGCTGGATCATGAAAACCGGACAGATGATATAACAATCATCATCGTTCACATCAAGGGCTTCTCTAGTTCAGGCGCTGCTGCAATTGTTGGGCAGCATGGACTCCGCATAAGCCATGCAAGTTCAGAGTGGGGAAGCTCGGAGGCCTTTTGCTCCATTAGAAGTGATTTCTCAGATCCACAGGTCTCTCAGCATGTGGGCTTGATGAACAGGAACACAGCAATTGCTGTTCCATCTCTCACGCAGTACCGACCATTGGAAGTGGTGAGAAGTGGGTTAATGGGGAGTATGCCTTCCCCTAGCTGCTAAAAAATATTCTCGTTTCGCTTGTAACAAAGATATCTTATCGTCAATGTTGCTAGTCAGTCCTAACCACAAGAATATGTATGTACAAGGAAGAACATATTGCTGTCTCTCTATCTGAGAGTTGCTCCAAGCTAAAGCCAACGAAATTATTGTACACTTGGAAATGGTGGCAACGATGATAAATCGACGGTGTTTCTCATTGAATGGAATTCAGAGAGAAGGAAATCGACATGATGGTTTGGTTTGGTTTCTTAGATTCCTCATATTCCCTGGGTGGTTTATGAAGGCCTTTTCCTGAACTTTGTATATATGATGATATTGTACAGAGATTCTTGACCGC

Coding sequence (CDS)

ATGAGAATCTTAGAAGAGGTTTTCTTGCAAGAAACTTGTTCTTGCACGAATTTGGTTCTTGGAAGAGAATCCATGGGTTGTGTTCTTGGAAAGTGTTTAACTTCCAGTCGCTACCCGCGGCCTTCTGACGGCGATATGACCCAATCCTGCGATGCTCCGCCGTGTAGTTTAGAAGGAAAACATGTACTAACGGGAAGGTTATTGGAGTCTGTTCGTGTGCCTTCTCATAACTTCCGTTTGGAGTACTCGCTCCTGACCCAGCGCGGCTACTACCCTGATTCCCCTGATAAAGAAAACCAAGACAGCTTCTGTGTCAGTACACAAATGGGAGGTAATCCGAATATCCATTTATTTGGTGTCTTTGATGGCCATGGTCAATTCGGATCGCTGTGTTCTAATTTCGTTAAGGAGAAGATTGTGCAAATGCTATGTAACGATTCTAAATTGTTGGAAGATCCTGTTAAAGCTTATAATTCGGCATTTTTGACCTTAAATGATGAATTGCGTAGTAGTGATATTGATGATTCCATGAGCGGCACTACAGCCATTACTGCTCTTGTTCTTGGGAATACCCTTTACGTTGCCAATGTGGGCGATTCGAGGGCAGTAATGGCTGTTAGAGTTGGGAATCGAATTGTTGCCGAGGATATGTCCCATGATCAAACACCATTTAGGAAGGATGAGTGCGAGAGAGTAAAGCTGGCCGGGGCTAGAGTGTTGACCATTGATCAAATTGAAGGGCTTAAAGATCCGGATATTCAAGTATGGGGTGATGAGGAGACCGAGGGAAATGATCCTCCTCGATTGTGGTTTGCTGATAGCTTGTACCCTGGTACTGCATTTACACGGAGCGTAGGAGACAGTACAGCGGAGAAGATTGGTGTCACTGCTGTCCCTGAGGTTTCAGTAGTTGAGCTGACTCAAAACCATCTTTTCTTTGTTGTTGCAAGCGATGGAGTTTTCGAGTTCCTTACGAGCCAAGCTGTTGTTGATATGGCTGCAAGGTATACCGATCCACGGGATGCATGCTCAGCTATTGCTGGAGAATCATATAAGCTATGGCTGGATCATGAAAACCGGACAGATGATATAACAATCATCATCGTTCACATCAAGGGCTTCTCTAGTTCAGGCGCTGCTGCAATTGTTGGGCAGCATGGACTCCGCATAAGCCATGCAAGTTCAGAGTGGGGAAGCTCGGAGGCCTTTTGCTCCATTAGAAGTGATTTCTCAGATCCACAGGTCTCTCAGCATGTGGGCTTGATGAACAGGAACACAGCAATTGCTGTTCCATCTCTCACGCAGTACCGACCATTGGAAGTGGTGAGAAGTGGGTTAATGGGGAGTATGCCTTCCCCTAGCTGCTAA

Protein sequence

MRILEEVFLQETCSCTNLVLGRESMGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSLLTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLCNDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVMAVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVGQHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEVVRSGLMGSMPSPSC
Homology
BLAST of CmaCh14G016650 vs. ExPASy Swiss-Prot
Match: Q7XJ53 (Probable protein phosphatase 2C 35 OS=Arabidopsis thaliana OX=3702 GN=At3g06270 PE=2 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.8e-135
Identity = 241/356 (67.70%), Postives = 281/356 (78.93%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYP-RPSDGDMTQSCDAPPCSLEGKHVLTG---RLLESVRVPSHNFRL 84
           MGCV  KC   SRYP   SDGD           LE   VL G   + L S+ VPS NF +
Sbjct: 1   MGCVQCKC--CSRYPSSSSDGDSRG-------PLEANGVLKGKDQKPLGSIHVPSPNFDM 60

Query: 85  EYSLLTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIV 144
            YS+L+QRGYYPDSPDKENQD++C+ T++ GNPN+H FGVFDGHG  G+ CSNFVKE++V
Sbjct: 61  VYSVLSQRGYYPDSPDKENQDTYCIKTELQGNPNVHFFGVFDGHGVLGTQCSNFVKERVV 120

Query: 145 QMLCNDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDS 204
           +ML  D  LLEDP KAY SAFL +N+EL  S+IDDSMSGTTAIT LV+G+ +YVANVGDS
Sbjct: 121 EMLSEDPTLLEDPEKAYKSAFLRVNEELHDSEIDDSMSGTTAITVLVVGDKIYVANVGDS 180

Query: 205 RAVMAVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEE 264
           RAV+AV+  NRI+AED+S+DQTPFRKDECERVK  GARVL++DQ+EGLKDP+IQ W +EE
Sbjct: 181 RAVLAVKDRNRILAEDLSYDQTPFRKDECERVKACGARVLSVDQVEGLKDPNIQTWANEE 240

Query: 265 TEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGV 324
           +EG DPPRLW  + +YPGTAFTRSVGD TAE IGV A PEVS+V L+ NHLFFVVASDG+
Sbjct: 241 SEGGDPPRLWVQNGMYPGTAFTRSVGDFTAESIGVIAEPEVSMVHLSPNHLFFVVASDGI 300

Query: 325 FEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSS 377
           FEFL SQAVVDM  RY DPRD C+A A ESYKLWL+HENRTDDITIIIV IK  S+
Sbjct: 301 FEFLPSQAVVDMVGRYADPRDGCAAAAAESYKLWLEHENRTDDITIIIVQIKKLSN 347

BLAST of CmaCh14G016650 vs. ExPASy Swiss-Prot
Match: Q9SL76 (Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=At2g20050/At2g20040 PE=2 SV=2)

HSP 1 Score: 398.7 bits (1023), Expect = 9.0e-110
Identity = 192/303 (63.37%), Postives = 233/303 (76.90%), Query Frame = 0

Query: 71  VRVPSHNFRLEYSLLTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSL 130
           V+VPS N+ L  S L+QRGYYPD+ DK NQDSF + T  G N + H FGVFDGHG+FG+ 
Sbjct: 97  VKVPSCNYELRCSFLSQRGYYPDALDKANQDSFAIHTPFGSNSDDHFFGVFDGHGEFGAQ 156

Query: 131 CSNFVKEKIVQMLCNDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGN 190
           CS FVK ++ + L    +   DP +A NSAFLT N +L +  +DDSMSGTTAIT +V G 
Sbjct: 157 CSQFVKRRLCENLLRHGRFRVDPAEACNSAFLTTNSQLHADLVDDSMSGTTAITVMVRGR 216

Query: 191 TLYVANVGDSRAVMAVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKD 250
           T+YVAN GDSRAV+A +    +VA D+S DQTPFR DE ERVKL GARVLT+DQIEGLK+
Sbjct: 217 TIYVANAGDSRAVLAEKRDGDLVAVDLSIDQTPFRPDELERVKLCGARVLTLDQIEGLKN 276

Query: 251 PDIQVWGDEETEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNH 310
           PD+Q WG EE +  DPPRLW  + +YPGTAFTRS+GDS AE IGV A PE++VVELT ++
Sbjct: 277 PDVQCWGTEEDDDGDPPRLWVPNGMYPGTAFTRSIGDSIAETIGVVANPEIAVVELTPDN 336

Query: 311 LFFVVASDGVFEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVH 370
            FFVVASDGVFEF++SQ VVDM A++ DPRDAC+AI  ESY+LWL +E RTDDITII+VH
Sbjct: 337 PFFVVASDGVFEFISSQTVVDMVAKHKDPRDACAAIVAESYRLWLQYETRTDDITIIVVH 396

Query: 371 IKG 374
           I G
Sbjct: 397 IDG 399

BLAST of CmaCh14G016650 vs. ExPASy Swiss-Prot
Match: Q8H2T0 (Probable protein phosphatase 2C 65 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0646100 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 4.4e-96
Identity = 186/360 (51.67%), Postives = 235/360 (65.28%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGC  GKC    R      G           ++ G+    G  L  V VP     LEY+ 
Sbjct: 1   MGCAQGKCCVPRRQRGRGGGG----------AVGGR---GGATLGRVAVPGAGLVLEYAT 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           L   G YPDSP +E+QD+  V+T+  G+P++HLF VFDGHG  G+ C+ F ++ + ++L 
Sbjct: 61  LAVAGLYPDSPGRESQDAHLVATRFAGHPDLHLFAVFDGHGACGAACAGFARDALPRLLA 120

Query: 145 -----------NDSKLLEDPVKAYNSAFLTLNDELRSSD-IDDSMSGTTAITALVLGNTL 204
                          ++EDP  A+  A    N E+ ++D +DDSMSGTTA+ ALV G  L
Sbjct: 121 GVGVGAGEEGGGRMVVVEDPAAAFREALPAANAEMHAADEVDDSMSGTTAVAALVAGGAL 180

Query: 205 YVANVGDSRAVMAVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPD 264
           +VANVGDSRAV  V    R+ AE++S DQTPFR DE  RVK  GARV++++Q+EG++DP+
Sbjct: 181 HVANVGDSRAVAGVWREGRVAAEELSWDQTPFRADERARVKACGARVMSVEQVEGVRDPE 240

Query: 265 IQVWGDEETEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLF 324
            + W  +E    DPPR+W  D LYPGTAFTRS+GD  AE +GV A PEV  VE+T  HLF
Sbjct: 241 AESWVADE---GDPPRVWARDGLYPGTAFTRSLGDQAAEAVGVIAEPEVKSVEITPAHLF 300

Query: 325 FVVASDGVFEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIK 373
           FVVASDGVFEFL+SQ VVDM A Y DPR+ACSAIA ESYKLWL+HENRTDDITIIIVHI+
Sbjct: 301 FVVASDGVFEFLSSQDVVDMVAAYEDPREACSAIAAESYKLWLEHENRTDDITIIIVHIR 344

BLAST of CmaCh14G016650 vs. ExPASy Swiss-Prot
Match: Q8H4S6 (Probable protein phosphatase 2C 64 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0566200 PE=2 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 3.3e-27
Identity = 87/286 (30.42%), Postives = 142/286 (49.65%), Query Frame = 0

Query: 99  NQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLCNDSK--LLEDPV-- 158
           NQD+  V    GG  +  L GVFDGHG  G + +  V++ +   L + ++    + P   
Sbjct: 66  NQDAMLVWDGFGGEDDGVLCGVFDGHGPHGHVVARRVRDSLPLRLMSAARDSGADMPAAA 125

Query: 159 --KAYNSAFLTLNDELRS-SDIDDSMSGTTAITALVLGNTLYVANVGDSRAVMAVR--VG 218
             KA+  A+  ++ +LRS   +D   SG+TA+T L LG+ LY+AN+GDSRAV+  R   G
Sbjct: 126 WRKAFARAYKAMDKDLRSHPSLDCFCSGSTAVTVLKLGSDLYMANIGDSRAVLGSREATG 185

Query: 219 NRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGNDPPRL 278
             +VA  ++ D  P    E ER+K    RV  +       +P++             PR+
Sbjct: 186 GGMVAVQLTVDLKPDVPSEAERIKKCRGRVFALQD-----EPEV-------------PRV 245

Query: 279 WFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFLTSQAV 338
           W      PG A  R+ GD   +  GV +VPE     LT+   F ++ASDGV++ L++Q  
Sbjct: 246 WLPFDDAPGLAMARAFGDFCLKDYGVISVPEFFHWSLTEKDQFVILASDGVWDVLSNQEA 305

Query: 339 VDMAARYTDPRDACSAIAGESYKLWLDH--ENRTDDITIIIVHIKG 374
           VD+ +       A  ++   + + W      ++ DD  ++ +++ G
Sbjct: 306 VDIVSASPSRSKAAKSLVEAATREWKTKYPTSKIDDCAVVCLYLDG 333

BLAST of CmaCh14G016650 vs. ExPASy Swiss-Prot
Match: Q2R637 (Probable protein phosphatase 2C 75 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0417400 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 5.8e-24
Identity = 89/334 (26.65%), Postives = 146/334 (43.71%), Query Frame = 0

Query: 99  NQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC-------------- 158
           NQD+        G  +    GVFDGHG  G L +  V++ +   LC              
Sbjct: 57  NQDAMVAWENYNGRSDTVFCGVFDGHGPHGHLIARKVRDILPSRLCDLIYEDCGDSPTSN 116

Query: 159 NDSKLLEDPVKAY---------------------------NSAFLTLNDELR-SSDIDDS 218
           +D   LE+ +  Y                             AF  ++ EL+   +ID  
Sbjct: 117 SDVSTLEENLSPYADAECRSPTLAGQKEHQEFFNAMKESFRKAFKNVDKELKLQRNIDSI 176

Query: 219 MSGTTAITALVLGNTLYVANVGDSRAVMAVRVGN-RIVAEDMSHDQTPFRKDECERVKLA 278
            SGTTA+T +  G+ L V N+GDSRAV+  R  N ++VA  ++ D  P    E  R++  
Sbjct: 177 CSGTTAVTLIKQGHDLIVGNLGDSRAVLGTRDQNDKLVAHQLTVDLKPDHPREARRIRRC 236

Query: 279 GARVLTIDQIEGLKDPDIQVWGDEETEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGV 338
             RV          +PD+              RLW  +   PG A  R+ GD   +  G+
Sbjct: 237 NGRVFAHQD-----EPDV-------------ARLWLPNCNSPGLAMARAFGDFCLKDFGL 296

Query: 339 TAVPEVSVVELTQNHLFFVVASDGVFEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWL 388
            +VP+V+  ++T+   F V+A+DGV++ L++Q VVD+ A  +    A  ++   + + W 
Sbjct: 297 ISVPDVTYRQITEKDEFIVLATDGVWDVLSNQEVVDVVASCSGRFAAARSVVDLANETWR 356

BLAST of CmaCh14G016650 vs. ExPASy TrEMBL
Match: A0A6J1IS80 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111480068 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 5.1e-241
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL
Sbjct: 1   MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC
Sbjct: 61  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM
Sbjct: 121 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN
Sbjct: 181 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL
Sbjct: 241 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG
Sbjct: 301 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 360

Query: 385 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 442
           QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV
Sbjct: 361 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 417

BLAST of CmaCh14G016650 vs. ExPASy TrEMBL
Match: A0A6J1IUJ5 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111480068 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 5.1e-241
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL
Sbjct: 1   MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC
Sbjct: 61  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM
Sbjct: 121 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN
Sbjct: 181 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL
Sbjct: 241 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG
Sbjct: 301 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 360

Query: 385 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 442
           QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV
Sbjct: 361 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 417

BLAST of CmaCh14G016650 vs. ExPASy TrEMBL
Match: A0A6J1GWT6 (Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111457573 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 3.8e-236
Identity = 409/417 (98.08%), Postives = 412/417 (98.80%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL
Sbjct: 1   MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQDSFCVSTQM GNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQ+LC
Sbjct: 61  LTQRGYYPDSPDKENQDSFCVSTQMRGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQILC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM
Sbjct: 121 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN
Sbjct: 181 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWF DSLYPGTAFTRSVGDS AEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL
Sbjct: 241 DPPRLWFPDSLYPGTAFTRSVGDSAAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQ VVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG
Sbjct: 301 TSQTVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 360

Query: 385 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 442
           Q+GLRISHASSEWGSSE FCSIRSDFSDPQVS+HVGLMNRNTAIAVPSLTQYRPLEV
Sbjct: 361 QNGLRISHASSEWGSSEPFCSIRSDFSDPQVSKHVGLMNRNTAIAVPSLTQYRPLEV 417

BLAST of CmaCh14G016650 vs. ExPASy TrEMBL
Match: A0A6J1GVL9 (Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111457573 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 3.8e-236
Identity = 409/417 (98.08%), Postives = 412/417 (98.80%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL
Sbjct: 1   MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQDSFCVSTQM GNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQ+LC
Sbjct: 61  LTQRGYYPDSPDKENQDSFCVSTQMRGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQILC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM
Sbjct: 121 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN
Sbjct: 181 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWF DSLYPGTAFTRSVGDS AEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL
Sbjct: 241 DPPRLWFPDSLYPGTAFTRSVGDSAAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQ VVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG
Sbjct: 301 TSQTVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 360

Query: 385 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 442
           Q+GLRISHASSEWGSSE FCSIRSDFSDPQVS+HVGLMNRNTAIAVPSLTQYRPLEV
Sbjct: 361 QNGLRISHASSEWGSSEPFCSIRSDFSDPQVSKHVGLMNRNTAIAVPSLTQYRPLEV 417

BLAST of CmaCh14G016650 vs. ExPASy TrEMBL
Match: A0A1S3AXU8 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103483765 PE=3 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.4e-198
Identity = 343/426 (80.52%), Postives = 381/426 (89.44%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCV+GKC TSS YP  +DGD+++  DAPPCSL GKH+LTGR LESVRVPSH + LEYSL
Sbjct: 1   MGCVMGKCCTSSHYPPSTDGDISRFGDAPPCSLHGKHILTGRTLESVRVPSHMYNLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQD+FCVST + GNPN+H+FGVFDGHGQFG  CSNFVK+++VQ+LC
Sbjct: 61  LTQRGYYPDSPDKENQDNFCVSTHIQGNPNVHIFGVFDGHGQFGMQCSNFVKDRVVQLLC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDS+LLEDPVKAYNSAFLT NDELR+S+IDDSMSGTTAIT LV+GNTLYVANVGDSRAV+
Sbjct: 121 NDSRLLEDPVKAYNSAFLTANDELRNSEIDDSMSGTTAITVLVIGNTLYVANVGDSRAVV 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AV+ GNRIVA+DMSHDQTPFRKDECERVKLAGARVLTIDQIEGL+DPDIQVWGDEE+EGN
Sbjct: 181 AVKNGNRIVAQDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLRDPDIQVWGDEESEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWF +SLYPGTAFTRSVGDSTAEKIGV AVPEVSVV+LT NHLFFV+ASDGVFEFL
Sbjct: 241 DPPRLWFPNSLYPGTAFTRSVGDSTAEKIGVIAVPEVSVVQLTPNHLFFVIASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQAVVDMAARY+DPRDACSAIAGESYKLWL+HENRTDDITIIIVHIKG S+SG +  VG
Sbjct: 301 TSQAVVDMAARYSDPRDACSAIAGESYKLWLEHENRTDDITIIIVHIKGLSNSGGSGTVG 360

Query: 385 QHGLRISHASSE---------WGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQ 442
            +G+ I H++SE          G+SE F S RSDFSD  VSQHVGLMNR+TAI VPS  Q
Sbjct: 361 LNGVCIRHSNSERGTSEISATTGTSEVFRSFRSDFSDLHVSQHVGLMNRSTAIVVPSPAQ 420

BLAST of CmaCh14G016650 vs. NCBI nr
Match: XP_022980782.1 (probable protein phosphatase 2C 35 isoform X2 [Cucurbita maxima])

HSP 1 Score: 843.2 bits (2177), Expect = 1.1e-240
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL
Sbjct: 1   MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC
Sbjct: 61  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM
Sbjct: 121 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN
Sbjct: 181 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL
Sbjct: 241 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG
Sbjct: 301 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 360

Query: 385 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 442
           QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV
Sbjct: 361 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 417

BLAST of CmaCh14G016650 vs. NCBI nr
Match: XP_022980781.1 (probable protein phosphatase 2C 35 isoform X1 [Cucurbita maxima])

HSP 1 Score: 843.2 bits (2177), Expect = 1.1e-240
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL
Sbjct: 1   MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC
Sbjct: 61  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM
Sbjct: 121 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN
Sbjct: 181 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL
Sbjct: 241 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG
Sbjct: 301 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 360

Query: 385 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 442
           QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV
Sbjct: 361 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 417

BLAST of CmaCh14G016650 vs. NCBI nr
Match: XP_023526069.1 (probable protein phosphatase 2C 35 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.0 bits (2148), Expect = 2.4e-237
Identity = 412/417 (98.80%), Postives = 414/417 (99.28%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL
Sbjct: 1   MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQDSFCVSTQM GNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQ+LC
Sbjct: 61  LTQRGYYPDSPDKENQDSFCVSTQMRGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQILC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM
Sbjct: 121 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN
Sbjct: 181 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWF DSLYPGTAFTRSVGDS AEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL
Sbjct: 241 DPPRLWFPDSLYPGTAFTRSVGDSAAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG
Sbjct: 301 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 360

Query: 385 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 442
           Q+GLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV
Sbjct: 361 QNGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 417

BLAST of CmaCh14G016650 vs. NCBI nr
Match: KAG7018665.1 (putative protein phosphatase 2C 35, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 828.6 bits (2139), Expect = 2.7e-236
Identity = 410/417 (98.32%), Postives = 412/417 (98.80%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL
Sbjct: 1   MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQDSFCVSTQM GNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQ+LC
Sbjct: 61  LTQRGYYPDSPDKENQDSFCVSTQMRGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQILC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM
Sbjct: 121 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN
Sbjct: 181 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWF DSLYPGTAFTRSVGDS AEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL
Sbjct: 241 DPPRLWFPDSLYPGTAFTRSVGDSAAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQ VVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG
Sbjct: 301 TSQTVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 360

Query: 385 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 442
           Q+GLRISHASSEWGSSE FCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV
Sbjct: 361 QNGLRISHASSEWGSSEPFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 417

BLAST of CmaCh14G016650 vs. NCBI nr
Match: XP_022955625.1 (probable protein phosphatase 2C 35 isoform X2 [Cucurbita moschata])

HSP 1 Score: 827.0 bits (2135), Expect = 7.9e-236
Identity = 409/417 (98.08%), Postives = 412/417 (98.80%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 84
           MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL
Sbjct: 1   MGCVLGKCLTSSRYPRPSDGDMTQSCDAPPCSLEGKHVLTGRLLESVRVPSHNFRLEYSL 60

Query: 85  LTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQMLC 144
           LTQRGYYPDSPDKENQDSFCVSTQM GNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQ+LC
Sbjct: 61  LTQRGYYPDSPDKENQDSFCVSTQMRGNPNIHLFGVFDGHGQFGSLCSNFVKEKIVQILC 120

Query: 145 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 204
           NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM
Sbjct: 121 NDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDSRAVM 180

Query: 205 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 264
           AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN
Sbjct: 181 AVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEETEGN 240

Query: 265 DPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 324
           DPPRLWF DSLYPGTAFTRSVGDS AEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL
Sbjct: 241 DPPRLWFPDSLYPGTAFTRSVGDSAAEKIGVTAVPEVSVVELTQNHLFFVVASDGVFEFL 300

Query: 325 TSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 384
           TSQ VVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG
Sbjct: 301 TSQTVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSSSGAAAIVG 360

Query: 385 QHGLRISHASSEWGSSEAFCSIRSDFSDPQVSQHVGLMNRNTAIAVPSLTQYRPLEV 442
           Q+GLRISHASSEWGSSE FCSIRSDFSDPQVS+HVGLMNRNTAIAVPSLTQYRPLEV
Sbjct: 361 QNGLRISHASSEWGSSEPFCSIRSDFSDPQVSKHVGLMNRNTAIAVPSLTQYRPLEV 417

BLAST of CmaCh14G016650 vs. TAIR 10
Match: AT3G06270.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 483.4 bits (1243), Expect = 2.0e-136
Identity = 241/356 (67.70%), Postives = 281/356 (78.93%), Query Frame = 0

Query: 25  MGCVLGKCLTSSRYP-RPSDGDMTQSCDAPPCSLEGKHVLTG---RLLESVRVPSHNFRL 84
           MGCV  KC   SRYP   SDGD           LE   VL G   + L S+ VPS NF +
Sbjct: 1   MGCVQCKC--CSRYPSSSSDGDSRG-------PLEANGVLKGKDQKPLGSIHVPSPNFDM 60

Query: 85  EYSLLTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKIV 144
            YS+L+QRGYYPDSPDKENQD++C+ T++ GNPN+H FGVFDGHG  G+ CSNFVKE++V
Sbjct: 61  VYSVLSQRGYYPDSPDKENQDTYCIKTELQGNPNVHFFGVFDGHGVLGTQCSNFVKERVV 120

Query: 145 QMLCNDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGNTLYVANVGDS 204
           +ML  D  LLEDP KAY SAFL +N+EL  S+IDDSMSGTTAIT LV+G+ +YVANVGDS
Sbjct: 121 EMLSEDPTLLEDPEKAYKSAFLRVNEELHDSEIDDSMSGTTAITVLVVGDKIYVANVGDS 180

Query: 205 RAVMAVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQVWGDEE 264
           RAV+AV+  NRI+AED+S+DQTPFRKDECERVK  GARVL++DQ+EGLKDP+IQ W +EE
Sbjct: 181 RAVLAVKDRNRILAEDLSYDQTPFRKDECERVKACGARVLSVDQVEGLKDPNIQTWANEE 240

Query: 265 TEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFVVASDGV 324
           +EG DPPRLW  + +YPGTAFTRSVGD TAE IGV A PEVS+V L+ NHLFFVVASDG+
Sbjct: 241 SEGGDPPRLWVQNGMYPGTAFTRSVGDFTAESIGVIAEPEVSMVHLSPNHLFFVVASDGI 300

Query: 325 FEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVHIKGFSS 377
           FEFL SQAVVDM  RY DPRD C+A A ESYKLWL+HENRTDDITIIIV IK  S+
Sbjct: 301 FEFLPSQAVVDMVGRYADPRDGCAAAAAESYKLWLEHENRTDDITIIIVQIKKLSN 347

BLAST of CmaCh14G016650 vs. TAIR 10
Match: AT2G20050.1 (protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent protein kinase regulators;ATP binding;protein serine/threonine phosphatases )

HSP 1 Score: 398.7 bits (1023), Expect = 6.4e-111
Identity = 192/303 (63.37%), Postives = 233/303 (76.90%), Query Frame = 0

Query: 71  VRVPSHNFRLEYSLLTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSL 130
           V+VPS N+ L  S L+QRGYYPD+ DK NQDSF + T  G N + H FGVFDGHG+FG+ 
Sbjct: 97  VKVPSCNYELRCSFLSQRGYYPDALDKANQDSFAIHTPFGSNSDDHFFGVFDGHGEFGAQ 156

Query: 131 CSNFVKEKIVQMLCNDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGN 190
           CS FVK ++ + L    +   DP +A NSAFLT N +L +  +DDSMSGTTAIT +V G 
Sbjct: 157 CSQFVKRRLCENLLRHGRFRVDPAEACNSAFLTTNSQLHADLVDDSMSGTTAITVMVRGR 216

Query: 191 TLYVANVGDSRAVMAVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKD 250
           T+YVAN GDSRAV+A +    +VA D+S DQTPFR DE ERVKL GARVLT+DQIEGLK+
Sbjct: 217 TIYVANAGDSRAVLAEKRDGDLVAVDLSIDQTPFRPDELERVKLCGARVLTLDQIEGLKN 276

Query: 251 PDIQVWGDEETEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNH 310
           PD+Q WG EE +  DPPRLW  + +YPGTAFTRS+GDS AE IGV A PE++VVELT ++
Sbjct: 277 PDVQCWGTEEDDDGDPPRLWVPNGMYPGTAFTRSIGDSIAETIGVVANPEIAVVELTPDN 336

Query: 311 LFFVVASDGVFEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVH 370
            FFVVASDGVFEF++SQ VVDM A++ DPRDAC+AI  ESY+LWL +E RTDDITII+VH
Sbjct: 337 PFFVVASDGVFEFISSQTVVDMVAKHKDPRDACAAIVAESYRLWLQYETRTDDITIIVVH 396

Query: 371 IKG 374
           I G
Sbjct: 397 IDG 399

BLAST of CmaCh14G016650 vs. TAIR 10
Match: AT2G20050.2 (protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent protein kinase regulators;ATP binding;protein serine/threonine phosphatases )

HSP 1 Score: 398.7 bits (1023), Expect = 6.4e-111
Identity = 192/303 (63.37%), Postives = 233/303 (76.90%), Query Frame = 0

Query: 71  VRVPSHNFRLEYSLLTQRGYYPDSPDKENQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSL 130
           V+VPS N+ L  S L+QRGYYPD+ DK NQDSF + T  G N + H FGVFDGHG+FG+ 
Sbjct: 97  VKVPSCNYELRCSFLSQRGYYPDALDKANQDSFAIHTPFGSNSDDHFFGVFDGHGEFGAQ 156

Query: 131 CSNFVKEKIVQMLCNDSKLLEDPVKAYNSAFLTLNDELRSSDIDDSMSGTTAITALVLGN 190
           CS FVK ++ + L    +   DP +A NSAFLT N +L +  +DDSMSGTTAIT +V G 
Sbjct: 157 CSQFVKRRLCENLLRHGRFRVDPAEACNSAFLTTNSQLHADLVDDSMSGTTAITVMVRGR 216

Query: 191 TLYVANVGDSRAVMAVRVGNRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKD 250
           T+YVAN GDSRAV+A +    +VA D+S DQTPFR DE ERVKL GARVLT+DQIEGLK+
Sbjct: 217 TIYVANAGDSRAVLAEKRDGDLVAVDLSIDQTPFRPDELERVKLCGARVLTLDQIEGLKN 276

Query: 251 PDIQVWGDEETEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNH 310
           PD+Q WG EE +  DPPRLW  + +YPGTAFTRS+GDS AE IGV A PE++VVELT ++
Sbjct: 277 PDVQCWGTEEDDDGDPPRLWVPNGMYPGTAFTRSIGDSIAETIGVVANPEIAVVELTPDN 336

Query: 311 LFFVVASDGVFEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWLDHENRTDDITIIIVH 370
            FFVVASDGVFEF++SQ VVDM A++ DPRDAC+AI  ESY+LWL +E RTDDITII+VH
Sbjct: 337 PFFVVASDGVFEFISSQTVVDMVAKHKDPRDACAAIVAESYRLWLQYETRTDDITIIVVH 396

Query: 371 IKG 374
           I G
Sbjct: 397 IDG 399

BLAST of CmaCh14G016650 vs. TAIR 10
Match: AT5G27930.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.6e-24
Identity = 81/305 (26.56%), Postives = 140/305 (45.90%), Query Frame = 0

Query: 99  NQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKI-VQMLCNDSKLLEDPV--- 158
           NQD   V    G   ++   G+FDGHG +G   +  V+  + + +LCN  K+L       
Sbjct: 74  NQDCALVWEGFGCQEDMIFCGIFDGHGPWGHYVAKQVRNSMPLSLLCNWQKILAQATLEP 133

Query: 159 ------------------KAYNSAFLTLNDEL-RSSDIDDSMSGTTAITALVLGNTLYVA 218
                             ++Y     T++ EL     ID   SGTTA+T +  G  +YVA
Sbjct: 134 ELDLEGSNKKISRFDIWKQSYLKTCATVDQELEHHRKIDSYYSGTTALTIVRQGEVIYVA 193

Query: 219 NVGDSRAVMAVRVG-NRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQ 278
           NVGDSRAV+A+      +VA  ++ D  P    E ER+     RV  +D   G+      
Sbjct: 194 NVGDSRAVLAMESDEGSLVAVQLTLDFKPNLPQEKERIIGCKGRVFCLDDEPGVH----- 253

Query: 279 VWGDEETEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFV 338
                        R+W  D+  PG A +R+ GD   ++ G+ +VPEV+   ++    F +
Sbjct: 254 -------------RVWQPDAETPGLAMSRAFGDYCIKEYGLVSVPEVTQRHISTKDHFII 313

Query: 339 VASDGVFEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWLDHEN--RTDDITIIIVHIK 378
           +ASDG+++ +++Q  +++ +   +   A   +  ++ + W         DD++++ + + 
Sbjct: 314 LASDGIWDVISNQEAIEIVSSTAERPKAAKRLVEQAVRAWKKKRRGYSMDDMSVVCLFLH 360

BLAST of CmaCh14G016650 vs. TAIR 10
Match: AT5G27930.2 (Protein phosphatase 2C family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.6e-24
Identity = 81/305 (26.56%), Postives = 140/305 (45.90%), Query Frame = 0

Query: 99  NQDSFCVSTQMGGNPNIHLFGVFDGHGQFGSLCSNFVKEKI-VQMLCNDSKLLEDPV--- 158
           NQD   V    G   ++   G+FDGHG +G   +  V+  + + +LCN  K+L       
Sbjct: 74  NQDCALVWEGFGCQEDMIFCGIFDGHGPWGHYVAKQVRNSMPLSLLCNWQKILAQATLEP 133

Query: 159 ------------------KAYNSAFLTLNDEL-RSSDIDDSMSGTTAITALVLGNTLYVA 218
                             ++Y     T++ EL     ID   SGTTA+T +  G  +YVA
Sbjct: 134 ELDLEGSNKKISRFDIWKQSYLKTCATVDQELEHHRKIDSYYSGTTALTIVRQGEVIYVA 193

Query: 219 NVGDSRAVMAVRVG-NRIVAEDMSHDQTPFRKDECERVKLAGARVLTIDQIEGLKDPDIQ 278
           NVGDSRAV+A+      +VA  ++ D  P    E ER+     RV  +D   G+      
Sbjct: 194 NVGDSRAVLAMESDEGSLVAVQLTLDFKPNLPQEKERIIGCKGRVFCLDDEPGVH----- 253

Query: 279 VWGDEETEGNDPPRLWFADSLYPGTAFTRSVGDSTAEKIGVTAVPEVSVVELTQNHLFFV 338
                        R+W  D+  PG A +R+ GD   ++ G+ +VPEV+   ++    F +
Sbjct: 254 -------------RVWQPDAETPGLAMSRAFGDYCIKEYGLVSVPEVTQRHISTKDHFII 313

Query: 339 VASDGVFEFLTSQAVVDMAARYTDPRDACSAIAGESYKLWLDHEN--RTDDITIIIVHIK 378
           +ASDG+++ +++Q  +++ +   +   A   +  ++ + W         DD++++ + + 
Sbjct: 314 LASDGIWDVISNQEAIEIVSSTAERPKAAKRLVEQAVRAWKKKRRGYSMDDMSVVCLFLH 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XJ532.8e-13567.70Probable protein phosphatase 2C 35 OS=Arabidopsis thaliana OX=3702 GN=At3g06270 ... [more]
Q9SL769.0e-11063.37Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing pr... [more]
Q8H2T04.4e-9651.67Probable protein phosphatase 2C 65 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q8H4S63.3e-2730.42Probable protein phosphatase 2C 64 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q2R6375.8e-2426.65Probable protein phosphatase 2C 75 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Match NameE-valueIdentityDescription
A0A6J1IS805.1e-241100.00Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111480068... [more]
A0A6J1IUJ55.1e-241100.00Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111480068... [more]
A0A6J1GWT63.8e-23698.08Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC1114575... [more]
A0A6J1GVL93.8e-23698.08Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC1114575... [more]
A0A1S3AXU82.4e-19880.52Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103483765 PE=... [more]
Match NameE-valueIdentityDescription
XP_022980782.11.1e-240100.00probable protein phosphatase 2C 35 isoform X2 [Cucurbita maxima][more]
XP_022980781.11.1e-240100.00probable protein phosphatase 2C 35 isoform X1 [Cucurbita maxima][more]
XP_023526069.12.4e-23798.80probable protein phosphatase 2C 35 [Cucurbita pepo subsp. pepo][more]
KAG7018665.12.7e-23698.32putative protein phosphatase 2C 35, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022955625.17.9e-23698.08probable protein phosphatase 2C 35 isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G06270.12.0e-13667.70Protein phosphatase 2C family protein [more]
AT2G20050.16.4e-11163.37protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent ... [more]
AT2G20050.26.4e-11163.37protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent ... [more]
AT5G27930.11.6e-2426.56Protein phosphatase 2C family protein [more]
AT5G27930.21.6e-2426.56Protein phosphatase 2C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 77..369
e-value: 1.3E-47
score: 174.2
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 106..347
e-value: 1.0E-36
score: 126.9
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 81..371
score: 39.911106
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 97..371
e-value: 2.63438E-56
score: 185.223
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 72..373
e-value: 4.1E-66
score: 225.4
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 68..373
NoneNo IPR availablePANTHERPTHR24353CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASEcoord: 61..440
NoneNo IPR availablePANTHERPTHR24353:SF127PROTEIN PHOSPHATASE 2C AND CYCLIC NUCLEOTIDE-BINDING/KINASE DOMAIN-CONTAINING PROTEINcoord: 61..440
IPR000222PPM-type phosphatase, divalent cation bindingPROSITEPS01032PPM_1coord: 117..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G016650.1CmaCh14G016650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0005952 cAMP-dependent protein kinase complex
molecular_function GO:0004691 cAMP-dependent protein kinase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0043169 cation binding
molecular_function GO:0016791 phosphatase activity