CmaCh14G014900 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G014900
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionHXXXD-type acyl-transferase family protein
LocationCma_Chr14: 11300827 .. 11303227 (+)
RNA-Seq ExpressionCmaCh14G014900
SyntenyCmaCh14G014900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGATCTAAAAGGAAGGCATGGAGGTGGAGATAATATCAAGTGAAACTATCAAACCATCATCCTTCCCACCATCTCCAAAATCCTTAAAACTCTCAGTTATTGATCAGCTCTCTCCCTTCACTTATACCTCCCTCCTCTTCTTCTATTCTCTCAAACACTCCCATGACCCTCTCCATCTCTCCCATCCCCTCAAAGTCTCCCTCTCCCGTGCCCTCTCCGACTTCTACCTTCTCGCCGGCGCCATCAAAGACAACACACACGTCCTCGACAATGGCGTCGGGGCCCTCTTTCAGGTTGCTAGAGTTCATCACACCATGCCCCAGTTCTTGAACCAACCGAGTTTTGAGTCGTTGAGTCAACTCATCCCTTTCCGATACATGCAAACCAGATCGTCGTCCACCATGGAAGCATGTAAGATAACTCTAATTTTTAATTATACATGTTAGAAGTGTACATTCAACCCGATAACCCGTACCAAAACTCAAATTATAGGGGTTGAATTGAGCTCGTATTTCGAATTAGGCTGGGTTCCTTTTTTTGAACCAAATTGATTCGGTTCGATTTTGGATTGGTTCATTAACGTCATACCAGTCTATTTGAGTAAAATATTTTTATATTTTTTATTTTGTTAGATATAATTTTTTAAATTAATCATTTTTATTGTTTTTTTAATTTTATTATATACTATTATATTATATTTTCATTTTTAGAGTAACTAATTCTTAATTATGTTTATGTTTAATTATATGGTTGATAGTTTTTATTAATGAATTTATTATTTATTTATTTTTTGTGTTTATCCAAATATTTGTATTTCTTTATGAATTTTAAAATCTATTATCTAAAATTGGATCGAATAATTAAGAATTGTGGATAAATAATTTTTTTATCTGAATCCAACCTGAAAATAAAAGGTTAGATTGGATTGTGAAAATTTGATTAGGTCGAGTTATCAATTCAGCTCACTCGTCCAAAAAAAATTATGTTAATATATATATATATAATATATATTAATTAACATAAATTTTTAATTTTTGGATCTACCAAGTATATCCTCAAGTCGCCGTCCAGCTCAACGCCTTTGTTGGCGGCGGGGTGGCAGTGGGGGTCTGTTTTTTACACAAGATCATCGACGGGACAACACTGAGCGGCTTTCTGAGGAGGTGGGCGTCCATGGCCGGCGGGTCAACAGAAGAGAAGGGAGGAGGGGAACAGCCGGAATACGGCGTGGCGGCAATGTTCCCGGGGAGAGAATTGTTGGTGGAAAATTCATGGCTTTCAAAAGGGTATTCGCCATTTGTAGGGGAAGGAAGGATTTGCAGAAAAAGGTTTGTTTTTGACGGCGCCGCCATTTCCGAGCTGAAAGCGGAAGTGAAAAGGAAGAGTGACAAGGTTCCCACAAGTGTGGAAGTGGTCACTGGTTTCATATGGAAGTATATGATGATAGCTGCAAGACAAAGCACGGGTTCTCAACGATCATCGGTAATGATTTAAAATTCCGAGATATCAAAGCACCGATTTTTATGTTCTTAAATATCAAAATTTCATTTTTCAAAATTTCGAGAGTTGATTTAACGCATTTTTATCCTTATCCTTATTACATTCACACCCTGATACTATGTTAATAATGATAAACCGACTGGTTACTCATTCTTAACATGGTGTCCAAGCAGGATCTTAGTGTTCTTAAATATCAAAATTTTCTTTAGACGGAATCAACCAAATTGAGCTTCAAAAATTTTGGAGGTTGATTTAACATATCCTTATTATATTCACAGCTATGATATCATATTAAGAAAGAGAAACAAAAGTGATACTCTCAATCCCACTAATGTATCTCATTCTTAACATAAATTAATAAAAATATTCTTAAACTTCTGGTAGGTACTGACTCATGCAGTCGACCTCCGGCGAAGAATGGCCCCGCCATTACCAGCGACCTCGATTGGAAACATTTTCTGGAGTGCGGTTGCTCATTACGACACACCGAAGGCCGAGATAGAATTGAGCGAGCTGGTTGATCTAGTTCAGCGATCGGTTGCGGAGATCGACGACGGGTTCATACGAGAAATGGCGGGAGAAGAAGGGTTTGAGGGAATATCAAGATGGTTCATGAGGATGAAGGAGCTTTATTCTTCAAAGCCATATTCATATGGGTTTACGAGCTGGCGAAATATGGGGCTAAACGAGGTTGATTTTGGGTGGGGAAAGGCTTCATGGGTGAGCTTTGCAGGACCTGAAAATTCAGTGCTGAAGAACGTTGTCGTTTTGAATGAAGGGATTTTGGGAGATGGGCTTGAAGCATGGATAATGTTGGATGAGGATGAAATGAACATATTGGAAAATGATCAACAATTTCTTGCTTTTGCTTCCTTTAATCCAACCATCATTTTAGCTTAA

mRNA sequence

TTGATCTAAAAGGAAGGCATGGAGGTGGAGATAATATCAAGTGAAACTATCAAACCATCATCCTTCCCACCATCTCCAAAATCCTTAAAACTCTCAGTTATTGATCAGCTCTCTCCCTTCACTTATACCTCCCTCCTCTTCTTCTATTCTCTCAAACACTCCCATGACCCTCTCCATCTCTCCCATCCCCTCAAAGTCTCCCTCTCCCGTGCCCTCTCCGACTTCTACCTTCTCGCCGGCGCCATCAAAGACAACACACACGTCCTCGACAATGGCGTCGGGGCCCTCTTTCAGGTTGCTAGAGTTCATCACACCATGCCCCAGTTCTTGAACCAACCGAGTTTTGAGTCGTTGAGTCAACTCATCCCTTTCCGATACATGCAAACCAGATCGTCGTCCACCATGGAAGCATTATATCCTCAAGTCGCCGTCCAGCTCAACGCCTTTGTTGGCGGCGGGGTGGCAGTGGGGGTCTGTTTTTTACACAAGATCATCGACGGGACAACACTGAGCGGCTTTCTGAGGAGGTGGGCGTCCATGGCCGGCGGGTCAACAGAAGAGAAGGGAGGAGGGGAACAGCCGGAATACGGCGTGGCGGCAATGTTCCCGGGGAGAGAATTGTTGGTGGAAAATTCATGGCTTTCAAAAGGGTATTCGCCATTTGTAGGGGAAGGAAGGATTTGCAGAAAAAGGTTTGTTTTTGACGGCGCCGCCATTTCCGAGCTGAAAGCGGAAGTGAAAAGGAAGAGTGACAAGGTTCCCACAAGTGTGGAAGTGGTCACTGGTTTCATATGGAAGTATATGATGATAGCTGCAAGACAAAGCACGGGTTCTCAACGATCATCGGTACTGACTCATGCAGTCGACCTCCGGCGAAGAATGGCCCCGCCATTACCAGCGACCTCGATTGGAAACATTTTCTGGAGTGCGGTTGCTCATTACGACACACCGAAGGCCGAGATAGAATTGAGCGAGCTGGTTGATCTAGTTCAGCGATCGGTTGCGGAGATCGACGACGGGTTCATACGAGAAATGGCGGGAGAAGAAGGGTTTGAGGGAATATCAAGATGGTTCATGAGGATGAAGGAGCTTTATTCTTCAAAGCCATATTCATATGGGTTTACGAGCTGGCGAAATATGGGGCTAAACGAGGTTGATTTTGGGTGGGGAAAGGCTTCATGGGTGAGCTTTGCAGGACCTGAAAATTCAGTGCTGAAGAACGTTGTCGTTTTGAATGAAGGGATTTTGGGAGATGGGCTTGAAGCATGGATAATGTTGGATGAGGATGAAATGAACATATTGGAAAATGATCAACAATTTCTTGCTTTTGCTTCCTTTAATCCAACCATCATTTTAGCTTAA

Coding sequence (CDS)

ATGGAGGTGGAGATAATATCAAGTGAAACTATCAAACCATCATCCTTCCCACCATCTCCAAAATCCTTAAAACTCTCAGTTATTGATCAGCTCTCTCCCTTCACTTATACCTCCCTCCTCTTCTTCTATTCTCTCAAACACTCCCATGACCCTCTCCATCTCTCCCATCCCCTCAAAGTCTCCCTCTCCCGTGCCCTCTCCGACTTCTACCTTCTCGCCGGCGCCATCAAAGACAACACACACGTCCTCGACAATGGCGTCGGGGCCCTCTTTCAGGTTGCTAGAGTTCATCACACCATGCCCCAGTTCTTGAACCAACCGAGTTTTGAGTCGTTGAGTCAACTCATCCCTTTCCGATACATGCAAACCAGATCGTCGTCCACCATGGAAGCATTATATCCTCAAGTCGCCGTCCAGCTCAACGCCTTTGTTGGCGGCGGGGTGGCAGTGGGGGTCTGTTTTTTACACAAGATCATCGACGGGACAACACTGAGCGGCTTTCTGAGGAGGTGGGCGTCCATGGCCGGCGGGTCAACAGAAGAGAAGGGAGGAGGGGAACAGCCGGAATACGGCGTGGCGGCAATGTTCCCGGGGAGAGAATTGTTGGTGGAAAATTCATGGCTTTCAAAAGGGTATTCGCCATTTGTAGGGGAAGGAAGGATTTGCAGAAAAAGGTTTGTTTTTGACGGCGCCGCCATTTCCGAGCTGAAAGCGGAAGTGAAAAGGAAGAGTGACAAGGTTCCCACAAGTGTGGAAGTGGTCACTGGTTTCATATGGAAGTATATGATGATAGCTGCAAGACAAAGCACGGGTTCTCAACGATCATCGGTACTGACTCATGCAGTCGACCTCCGGCGAAGAATGGCCCCGCCATTACCAGCGACCTCGATTGGAAACATTTTCTGGAGTGCGGTTGCTCATTACGACACACCGAAGGCCGAGATAGAATTGAGCGAGCTGGTTGATCTAGTTCAGCGATCGGTTGCGGAGATCGACGACGGGTTCATACGAGAAATGGCGGGAGAAGAAGGGTTTGAGGGAATATCAAGATGGTTCATGAGGATGAAGGAGCTTTATTCTTCAAAGCCATATTCATATGGGTTTACGAGCTGGCGAAATATGGGGCTAAACGAGGTTGATTTTGGGTGGGGAAAGGCTTCATGGGTGAGCTTTGCAGGACCTGAAAATTCAGTGCTGAAGAACGTTGTCGTTTTGAATGAAGGGATTTTGGGAGATGGGCTTGAAGCATGGATAATGTTGGATGAGGATGAAATGAACATATTGGAAAATGATCAACAATTTCTTGCTTTTGCTTCCTTTAATCCAACCATCATTTTAGCTTAA

Protein sequence

MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKVSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTEEKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEVKRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYSSKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIMLDEDEMNILENDQQFLAFASFNPTIILA
Homology
BLAST of CmaCh14G014900 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.3e-48
Identity = 144/439 (32.80%), Postives = 229/439 (52.16%), Query Frame = 0

Query: 2   EVEIISSETIKPSSFPPSPKSL---KLSVIDQLSPFTYTSLLFFY--SLKHSHDPLHLSH 61
           +++I+S E I+PSS  P+P++L   KLS +DQ+    +  ++ FY   L  + D    S 
Sbjct: 4   QMQILSEELIQPSS--PTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRSE 63

Query: 62  PLKVSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLI 121
            LK SLS  L+ FY LAG I  N+ V  N  G  F  ARVH  + + +   + + L+Q +
Sbjct: 64  NLKRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYL 123

Query: 122 PFRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAG 181
           PF+       S ++   P +AV+++ F  GG A+GVC  HKI D  +L+ FL  W +   
Sbjct: 124 PFQPYPGGEESGLKKDIP-LAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTA--- 183

Query: 182 GSTEEKGGGEQPEYGVAA-MFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISE 241
            + +E+    QP + + +  FP  E        S     F+ +  I  KRFVFD   +  
Sbjct: 184 -TCQEETDIVQPNFDLGSHHFPPME--------SIPAPEFLPDENIVMKRFVFDKEKLEA 243

Query: 242 LKAEVKRKSDKVPTS--VEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLP 301
           LKA++   + +V  S  V++V   IWK  +   R    ++   V   AV+LR RM PP P
Sbjct: 244 LKAQLASSATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAAQAVNLRSRMNPPFP 303

Query: 302 ATSIGNIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFM 361
            +++GNI   A   Y   + + + S+LV  ++ S+A+IDD  ++E+  ++G   +     
Sbjct: 304 QSAMGNI---ATMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVKEL--QKGVTYLD---- 363

Query: 362 RMKELYSSKPYS-YGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGD 421
                Y ++P   + F+SW  +G  ++DFGWGK   V         +KN+V L +    D
Sbjct: 364 -----YEAEPQELFSFSSWCRLGFYDLDFGWGKPVSVC---TTTVPMKNLVYLMDTRNED 410

Query: 422 GLEAWIMLDEDEMNILEND 432
           G+EAWI + EDEM++L +D
Sbjct: 424 GMEAWISMAEDEMSMLSSD 410

BLAST of CmaCh14G014900 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 7.7e-45
Identity = 156/473 (32.98%), Postives = 236/473 (49.89%), Query Frame = 0

Query: 3   VEIISSETIKPSSFPPSP-KSLKLSVIDQLSP-FTYTSLLFFY--------SLKHSHDPL 62
           VE+IS ETIKP++  PS  K+  LS++DQ  P + Y  ++ FY           + HD L
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 63  HLSHPLKVSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESL 122
            L   LK SLS+ L  FY +AG + DN  V  +  G  F   ++   M +F++QP    L
Sbjct: 69  DL---LKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQPDV-PL 128

Query: 123 SQLIPFRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWA 182
           SQL+P   +   +S   EAL   V VQ+N F  GG A+     HKI D  T+S F+R WA
Sbjct: 129 SQLLPSEVVS--ASVPKEAL---VIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWA 188

Query: 183 -----SMAGGSTEEKGGGEQ-PEYGVAAMFPGRELLVENSWLSKGYSPF------VGEGR 242
                S +GGST      +  P +  A++FP  E L   S +S+   PF        + +
Sbjct: 189 STTKTSRSGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSE--IPFSSTPEDTEDDK 248

Query: 243 ICRKRFVFDGAAISELKAEVK-----RKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRS 302
              KRFVFD A I+ ++ +++         +  T VEVVT  IWK +M    +ST +   
Sbjct: 249 TVSKRFVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVM----KSTPAGFL 308

Query: 303 SVLTHAVDLRRRMAPPLPATSIGNIFWSAVA-----------------HYDTPKAEIELS 362
            V+ HAV+LR++M PPL   S GN+  +  A                 +  + ++++ L 
Sbjct: 309 PVVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLH 368

Query: 363 ELVDLVQRSVAEIDDGFIREMAGEEG-FEGISRWF-----MRMKELYSSKPYSYGFTSWR 422
           EL D + +  +EID     ++ G++G  E + + F       +K++   +  ++  +SW 
Sbjct: 369 ELHDFIAQMRSEID-----KVKGDKGSLEKVIQNFASGHDASIKKINDVEVINFWISSWC 428

Query: 423 NMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIMLDEDEM 426
            MGL E+DFGWGK  WV+   P     KN   +N+   G+G+E W    ED+M
Sbjct: 429 RMGLYEIDFGWGKPIWVT-VDPNIKPNKNCFFMNDTKCGEGIEVWASFLEDDM 460

BLAST of CmaCh14G014900 vs. ExPASy Swiss-Prot
Match: O23393 (BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.4e-43
Identity = 141/450 (31.33%), Postives = 229/450 (50.89%), Query Frame = 0

Query: 2   EVEIISSETIKPSSFPPSPKS-LKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV 61
           ++E+   E IKP+S  PSP+  L+LS++D   P  Y S +FFY L      +  S  LK+
Sbjct: 4   KLEVTGKEVIKPAS--PSPRDRLQLSILDLYCPGIYVSTIFFYDLITESSEV-FSENLKL 63

Query: 62  SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY 121
           SLS  LS FY LAG I +   +  N  GA+F  AR    +P FL   + +SLS  +P   
Sbjct: 64  SLSETLSRFYPLAGRI-EGLSISCNDEGAVFTEARTDLLLPDFLRNLNTDSLSGFLP--- 123

Query: 122 MQTRSSSTMEALYPQVAVQLNAF-VGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGST 181
             T ++    A +P ++V++  F  G GVAV V   HKI D  +L  F++ WA+    +T
Sbjct: 124 --TLAAGESPAAWPLLSVKVTFFGSGSGVAVSVSVSHKICDIASLVTFVKDWAT----TT 183

Query: 182 EEKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAE 241
            +       E+    ++P     +   + S      +   +   KRFVF+ + I+ELK +
Sbjct: 184 AKGKSNSTIEFAETTIYPPPPSHMYEQFPSTDSDSNI-TSKYVLKRFVFEPSKIAELKHK 243

Query: 242 VKRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPL-PATSIG 301
              +S  VPT VE +   IW+    ++R +    R +V+  A+D+R R+   + P   IG
Sbjct: 244 AASESVPVPTRVEAIMSLIWRCARNSSRSNLLIPRQAVMWQAMDIRLRIPSSVAPKDVIG 303

Query: 302 NIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEE-GFEGISRWFMRMKE 361
           N+  S  +     ++E E+ E+V   +++   +++     + G   G   +S     + E
Sbjct: 304 NL-QSGFSLKKDAESEFEIPEIVATFRKNKERVNEMIKESLQGNTIGQSLLSLMAETVSE 363

Query: 362 LYSSKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNV--VVLNEGILGDGLE 421
             S++   Y  +SW      EVDFG G   WV +A   +++  N+  VVL +   GDG+E
Sbjct: 364 --STEIDRYIMSSWCRKPFYEVDFGSGSPVWVGYA--SHTIYDNMVGVVLIDSKEGDGVE 423

Query: 422 AWIMLDEDEMNILENDQQFLAFASFNPTII 446
           AWI L E++M++  +DQ+ LA+A  NP ++
Sbjct: 424 AWISLPEEDMSVFVDDQELLAYAVLNPPVV 434

BLAST of CmaCh14G014900 vs. ExPASy Swiss-Prot
Match: Q9FI40 (BAHD acyltransferase At5g47980 OS=Arabidopsis thaliana OX=3702 GN=BAHD1 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.5e-43
Identity = 142/462 (30.74%), Postives = 223/462 (48.27%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKH--SHDPLHLSHPL 60
           + +E+I  E IKPSS  P  + L+LSVID         ++FFY+L       P  +S  L
Sbjct: 3   LNLEVIQREVIKPSSPAPHDR-LQLSVIDFGIAEACVPMIFFYNLADLAEKSPDIVSTRL 62

Query: 61  KVSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPF 120
           + SLS+ALS FY LAG  K+   +  N  GA+F  AR +  + +FL      SL  LIP 
Sbjct: 63  RSSLSQALSRFYPLAGK-KEGVSISCNDEGAVFTEARTNLLLSEFLRNIDINSLKILIP- 122

Query: 121 RYMQTRSSSTMEALYPQVAVQLNAF-VGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGG 180
               T +        P ++VQ   F  G G+AVG+C  H I D  ++S F+R WA+ A G
Sbjct: 123 ----TLAPGESLDSRPLLSVQATFFGSGSGLAVGICVSHCICDAASVSTFVRGWAATARG 182

Query: 181 STEEKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRK----RFVFDGAAI 240
            + ++     P++   A+ P  ++ +         SPF     +  K    RFVF+   I
Sbjct: 183 DSNDE--LSTPQFAEVAIHPPADISIHG-------SPFNALSEVREKCVTNRFVFESDKI 242

Query: 241 SELKAEVKRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRM-APPL 300
           ++LK     KS   PT VE V   IW+    A+  +    R++++T ++DLR R+    L
Sbjct: 243 TKLKIVAASKSVPSPTRVEAVMSLIWRCARNASHANLIVPRATMMTQSMDLRLRIPTNVL 302

Query: 301 PATSIGNIFWSAVAHYDTPKAEIELSELVDLVQRSVAE--------IDDGFIREMAGEEG 360
              +IGN+          P +EIE+SE+V   ++   E        ++ G      G++ 
Sbjct: 303 SPDAIGNL-QGVFFLKRGPGSEIEISEVVAEFRKEKEEFNEMIKENVNGGHTNTTLGQKI 362

Query: 361 FEGISRWFMRMKELYSSKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVV 420
             GI+ +   +K        +Y  +SW      EVDFGWG+ +WV     +       V+
Sbjct: 363 MSGIANYMSELKPNID----TYTMSSWCRKAFYEVDFGWGRPAWVGLGHQDIQDGVMYVL 422

Query: 421 LNEGILGDGLEAWIMLDEDEMNILENDQQFLAFASFNPTIIL 447
           L +   G+G+E W+ + E +M     DQ+ L++AS NP +++
Sbjct: 423 LVDAKDGEGVEVWVGIPEQDMAAFVCDQELLSYASLNPPVLI 443

BLAST of CmaCh14G014900 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 169.1 bits (427), Expect = 1.1e-40
Identity = 148/451 (32.82%), Postives = 222/451 (49.22%), Query Frame = 0

Query: 2   EVEIISSETIKPSSFPPSPKSL---KLSVIDQLSPFTYTSLLFFY--SLKHSHDPLHLSH 61
           ++E +S E I PSS  P+P+SL   K+S +DQL    +   + FY   L  + DP   S 
Sbjct: 4   QMEKVSEELILPSS--PTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTSQ 63

Query: 62  PLKVSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFL-NQPSFESLSQL 121
            LK SLS+ L+ FY LAG I  N+ V  N  G  F  ARV   + Q + N    E L Q 
Sbjct: 64  HLKQSLSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQY 123

Query: 122 IPFRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMA 181
           +P            E +   +AV+++ F  GG A+GV   HKI D  +L+ FL  W +  
Sbjct: 124 LPSAAYPGGKIEVNEDV--PLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATC 183

Query: 182 GGSTEEKGGGEQPEYGVAAM-FPGRELLVENSWLSKGYSP-FVGEGRICRKRFVFDGAAI 241
            G TE       P + +AA  FP     V+N+      SP  V +  +  KRFVFD   I
Sbjct: 184 RGETEI----VLPNFDLAARHFPP----VDNT-----PSPELVPDENVVMKRFVFDKEKI 243

Query: 242 SELKAEVKRKSDKVPTS-VEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPL 301
             L+A+    S++   S V++V  +IWK+++   R   G++   V+  AV+LR RM PPL
Sbjct: 244 GALRAQASSASEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNKFVVVQAVNLRSRMNPPL 303

Query: 302 PATSIGNIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWF 361
           P  ++GNI     A  D  + + +  +L+  ++ S+ + +D    E+             
Sbjct: 304 PHYAMGNIATLLFAAVDA-EWDKDFPDLIGPLRTSLEKTEDDHNHEL------------L 363

Query: 362 MRMKELYSSKPYS-YGFTSWRNMGLNEVDFGWGK-ASWVSFAGPENSVLKNVVVLNEGIL 421
             M  LY  +P     FTSW  +G  ++DFGWGK  S  +   P+    +N  +L +   
Sbjct: 364 KGMTCLYELEPQELLSFTSWCRLGFYDLDFGWGKPLSACTTTFPK----RNAALLMDTRS 420

Query: 422 GDGLEAWIMLDEDEMNILENDQQFLAFASFN 442
           GDG+EAW+ + EDEM +L  +   L  + F+
Sbjct: 424 GDGVEAWLPMAEDEMAMLPVELLSLVDSDFS 420

BLAST of CmaCh14G014900 vs. ExPASy TrEMBL
Match: A0A6J1IPN4 (BAHD acyltransferase BIA1-like OS=Cucurbita maxima OX=3661 GN=LOC111479388 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 3.6e-255
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV 60
           MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV
Sbjct: 1   MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV 60

Query: 61  SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY 120
           SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY
Sbjct: 61  SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY 120

Query: 121 MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE 180
           MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE
Sbjct: 121 MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE 180

Query: 181 EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV 240
           EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV
Sbjct: 181 EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV 240

Query: 241 KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI 300
           KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI
Sbjct: 241 KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI 300

Query: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS 360
           FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS
Sbjct: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS 360

Query: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML 420
           SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML
Sbjct: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML 420

Query: 421 DEDEMNILENDQQFLAFASFNPTIILA 448
           DEDEMNILENDQQFLAFASFNPTIILA
Sbjct: 421 DEDEMNILENDQQFLAFASFNPTIILA 447

BLAST of CmaCh14G014900 vs. ExPASy TrEMBL
Match: A0A6J1GVJ3 (salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111457826 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.3e-238
Identity = 418/447 (93.51%), Postives = 430/447 (96.20%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV 60
           MEVEIISSETIKPSSFPPS +SLKLSV+DQLSPFTYTSL+FFYSLKHSH PLHLSHPLK 
Sbjct: 1   MEVEIISSETIKPSSFPPSLRSLKLSVLDQLSPFTYTSLVFFYSLKHSHVPLHLSHPLKA 60

Query: 61  SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY 120
           SLS ALSDFYLLAGAIKDNTH+LDNGVGALFQVARVH TMP+FLNQPSFESLSQLIPFRY
Sbjct: 61  SLSHALSDFYLLAGAIKDNTHILDNGVGALFQVARVHRTMPEFLNQPSFESLSQLIPFRY 120

Query: 121 MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE 180
           +QTRS STME  YPQVAVQLNAF GGGVAVGVCFLHKIIDGTTLSGFLRRWASMAG STE
Sbjct: 121 VQTRSLSTMEPSYPQVAVQLNAFSGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGVSTE 180

Query: 181 EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV 240
           EKGGGEQPEYG AA FPGRELLV NSWLSKGYSPFVGEGRICRKRFVFDGAAISELK EV
Sbjct: 181 EKGGGEQPEYGTAATFPGRELLVGNSWLSKGYSPFVGEGRICRKRFVFDGAAISELKEEV 240

Query: 241 KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI 300
           KRKSDK+PTSVEVVTGFIWKYMMIAARQS+ S+RSSVLTHAVDLRRRMAPPLPATSIGNI
Sbjct: 241 KRKSDKIPTSVEVVTGFIWKYMMIAARQSSRSRRSSVLTHAVDLRRRMAPPLPATSIGNI 300

Query: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS 360
           FWSAVAHYDTPKAEIELSELVDLVQRSVAEID+GFIREMAGEEGFEGISRWFMRMKELY+
Sbjct: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDEGFIREMAGEEGFEGISRWFMRMKELYT 360

Query: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML 420
           SKPYSYGFTSWRNMGLNEVDFGWGKASWVS AGPENSVLKNVVVL EGILGDG+EAWIML
Sbjct: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSVAGPENSVLKNVVVLKEGILGDGIEAWIML 420

Query: 421 DEDEMNILENDQQFLAFASFNPTIILA 448
           DEDEMNILENDQQFLAFASFNPTIILA
Sbjct: 421 DEDEMNILENDQQFLAFASFNPTIILA 447

BLAST of CmaCh14G014900 vs. ExPASy TrEMBL
Match: A0A5A7TN59 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001100 PE=3 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.4e-179
Identity = 346/460 (75.22%), Postives = 387/460 (84.13%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPS--PKSLKLSVIDQLSPFTYTSLLFFYSLKHSHD----PLHL 60
           MEVEIISSETIKPSSFP S   KS KLSV+DQLSPF+YTSL+FFY+L +       P +L
Sbjct: 1   MEVEIISSETIKPSSFPQSLHLKSFKLSVLDQLSPFSYTSLVFFYTLNNEESNYDTPHYL 60

Query: 61  SHPLKVSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQ 120
           S PLK SLS+ALSDFYLLAG IKDN  +L +GVGAL++VARVH  M +F NQ SFESLSQ
Sbjct: 61  SQPLKTSLSKALSDFYLLAGTIKDNKQILADGVGALYRVARVHGAMSKFFNQSSFESLSQ 120

Query: 121 LIPFRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASM 180
           L+PFR +Q   SSTMEA+ PQVAVQLNAF GGGVAVGVCFLHKIIDGTTLSGFLRRWA++
Sbjct: 121 LLPFRSVQIMPSSTMEAICPQVAVQLNAFNGGGVAVGVCFLHKIIDGTTLSGFLRRWATV 180

Query: 181 AGG-STEEKGGGEQPEYGVAA-MFPGRE-LLVENSWLSKGYSPFVGEG-RICRKRFVFDG 240
           AGG + EEKGG    EY   A  FPGR+ L + NSWLSKGYSPFVGEG RI R+RFVF+G
Sbjct: 181 AGGTAAEEKGGEGVAEYKAGAEAFPGRDSLFLGNSWLSKGYSPFVGEGIRIKRRRFVFEG 240

Query: 241 AAISELKAE-VKRKSDKVPTSVEVVTGFIWKYMMIAARQ-STGSQR-SSVLTHAVDLRRR 300
            AI ELK E +K K+   PTSVE+VT FIWKYMMIAARQ S+GSQR SSVLTHAVDLRRR
Sbjct: 241 DAILELKEELMKWKNVTNPTSVELVTAFIWKYMMIAARQRSSGSQRTSSVLTHAVDLRRR 300

Query: 301 MAPPLPATSIGNIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEG 360
           MAPPLP TS+GNIFWSAVAHYD+  AEIELS+LV+L+++S AEIDD FI+EM GEEGF+ 
Sbjct: 301 MAPPLPPTSMGNIFWSAVAHYDSMDAEIELSKLVNLLRQSFAEIDDKFIQEMEGEEGFQT 360

Query: 361 ISRWFMRMKELYSSKPYSYGFTSWRNMGLNEVDFGWG-KASWVSFAGPENSVLKNVVVLN 420
           IS+WFMRM+ELYSSKPYSYGFTSWRNMGLNE+DFGWG + SWVS AGPENSVLKN+VVL 
Sbjct: 361 ISKWFMRMQELYSSKPYSYGFTSWRNMGLNEIDFGWGRRPSWVSLAGPENSVLKNIVVLK 420

Query: 421 EGILGDGLEAWIMLDEDEMNILENDQQFLAFASFNPTIIL 447
           E  LGDG+EAWIMLDEDEMNILENDQQFLAFAS NPTI L
Sbjct: 421 EDTLGDGIEAWIMLDEDEMNILENDQQFLAFASHNPTIYL 460

BLAST of CmaCh14G014900 vs. ExPASy TrEMBL
Match: A0A1S3BWZ0 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494520 PE=3 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.4e-179
Identity = 346/460 (75.22%), Postives = 387/460 (84.13%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPS--PKSLKLSVIDQLSPFTYTSLLFFYSLKHSHD----PLHL 60
           MEVEIISSETIKPSSFP S   KS KLSV+DQLSPF+YTSL+FFY+L +       P +L
Sbjct: 1   MEVEIISSETIKPSSFPQSLHLKSFKLSVLDQLSPFSYTSLVFFYTLNNEESNYDTPHYL 60

Query: 61  SHPLKVSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQ 120
           S PLK SLS+ALSDFYLLAG IKDN  +L +GVGAL++VARVH  M +F NQ SFESLSQ
Sbjct: 61  SQPLKTSLSKALSDFYLLAGTIKDNKQILADGVGALYRVARVHGAMSKFFNQSSFESLSQ 120

Query: 121 LIPFRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASM 180
           L+PFR +Q   SSTMEA+ PQVAVQLNAF GGGVAVGVCFLHKIIDGTTLSGFLRRWA++
Sbjct: 121 LLPFRSVQIMPSSTMEAICPQVAVQLNAFNGGGVAVGVCFLHKIIDGTTLSGFLRRWATV 180

Query: 181 AGG-STEEKGGGEQPEYGVAA-MFPGRE-LLVENSWLSKGYSPFVGEG-RICRKRFVFDG 240
           AGG + EEKGG    EY   A  FPGR+ L + NSWLSKGYSPFVGEG RI R+RFVF+G
Sbjct: 181 AGGTAAEEKGGEGVAEYKAGAEAFPGRDSLFLGNSWLSKGYSPFVGEGIRIKRRRFVFEG 240

Query: 241 AAISELKAE-VKRKSDKVPTSVEVVTGFIWKYMMIAARQ-STGSQR-SSVLTHAVDLRRR 300
            AI ELK E +K K+   PTSVE+VT FIWKYMMIAARQ S+GSQR SSVLTHAVDLRRR
Sbjct: 241 DAILELKEELMKWKNVTNPTSVELVTAFIWKYMMIAARQRSSGSQRTSSVLTHAVDLRRR 300

Query: 301 MAPPLPATSIGNIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEG 360
           MAPPLP TS+GNIFWSAVAHYD+  AEIELS+LV+L+++S AEIDD FI+EM GEEGF+ 
Sbjct: 301 MAPPLPPTSMGNIFWSAVAHYDSMDAEIELSKLVNLLRQSFAEIDDKFIQEMEGEEGFQT 360

Query: 361 ISRWFMRMKELYSSKPYSYGFTSWRNMGLNEVDFGWG-KASWVSFAGPENSVLKNVVVLN 420
           IS+WFMRM+ELYSSKPYSYGFTSWRNMGLNE+DFGWG + SWVS AGPENSVLKN+VVL 
Sbjct: 361 ISKWFMRMQELYSSKPYSYGFTSWRNMGLNEIDFGWGRRPSWVSLAGPENSVLKNIVVLK 420

Query: 421 EGILGDGLEAWIMLDEDEMNILENDQQFLAFASFNPTIIL 447
           E  LGDG+EAWIMLDEDEMNILENDQQFLAFAS NPTI L
Sbjct: 421 EDTLGDGIEAWIMLDEDEMNILENDQQFLAFASHNPTIYL 460

BLAST of CmaCh14G014900 vs. ExPASy TrEMBL
Match: A0A0A0KZ24 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016450 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 5.5e-179
Identity = 345/461 (74.84%), Postives = 385/461 (83.51%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPS--PKSLKLSVIDQLSPFTYTSLLFFYSLKHSH-----DPLH 60
           MEVEIISSETIKPSSFP S   KS KLSV+DQLSPFTYTSL+FFY+L +         LH
Sbjct: 1   MEVEIISSETIKPSSFPQSLHLKSFKLSVLDQLSPFTYTSLVFFYTLNNEELSNEDTALH 60

Query: 61  LSHPLKVSLSRALS-DFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESL 120
           LS PLK SLS+ALS DFYLLAG IK N  +L NGVGAL+QVARV   M + LNQPSFESL
Sbjct: 61  LSQPLKTSLSKALSADFYLLAGTIKHNKQILANGVGALYQVARVQGAMSKVLNQPSFESL 120

Query: 121 SQLIPFRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWA 180
           SQL+PFR +Q  SSST EA+YPQVAVQLNAF GGGVAVGVCFLHKIIDGTTLSGFLRRWA
Sbjct: 121 SQLLPFRSLQIMSSSTKEAIYPQVAVQLNAFNGGGVAVGVCFLHKIIDGTTLSGFLRRWA 180

Query: 181 SMAGGS-TEEKGGGEQPEYGVAA-MFPGRELLVENSWLSKGYSPFVGEG-RICRKRFVFD 240
           ++AGGS  EEKGG    EY V A  FPGR+ L+ NSWLSKGYSPFVGEG RI R+RFVF+
Sbjct: 181 AVAGGSAAEEKGGEGVAEYTVGAEAFPGRDSLLGNSWLSKGYSPFVGEGIRIKRRRFVFE 240

Query: 241 GAAISELKAE-VKRKSDKVPTSVEVVTGFIWKYMMIAAR-QSTGSQR-SSVLTHAVDLRR 300
           G AI ELK E +K K  + PTSVE+VT FIWKY+MIAAR +S+GSQ+ SSVLTHAVDLRR
Sbjct: 241 GDAILELKEELMKWKDVRNPTSVELVTAFIWKYLMIAARKRSSGSQQISSVLTHAVDLRR 300

Query: 301 RMAPPLPATSIGNIFWSAVAHYD-TPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGF 360
           RMAPPLP TS+GNI WSAVAHYD T   EIELS+LV+L+  S  EID+ FI+EM GEEGF
Sbjct: 301 RMAPPLPPTSMGNILWSAVAHYDSTDDVEIELSKLVNLLWESFTEIDNKFIQEMEGEEGF 360

Query: 361 EGISRWFMRMKELYSSKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVL 420
           + IS+WFMRM+ELYSSKPY+YGFTSWRNMGLN++DFGWG+ SWVSFAGPENSVLKN+VVL
Sbjct: 361 QTISKWFMRMQELYSSKPYAYGFTSWRNMGLNDIDFGWGRPSWVSFAGPENSVLKNIVVL 420

Query: 421 NEGILGDGLEAWIMLDEDEMNILENDQQFLAFASFNPTIIL 447
            E  LGDG+EAWIMLDEDEMNILENDQQFLAFAS+NPTI L
Sbjct: 421 KEDNLGDGIEAWIMLDEDEMNILENDQQFLAFASYNPTIYL 461

BLAST of CmaCh14G014900 vs. NCBI nr
Match: XP_022979792.1 (BAHD acyltransferase BIA1-like [Cucurbita maxima])

HSP 1 Score: 890.2 bits (2299), Expect = 7.4e-255
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV 60
           MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV
Sbjct: 1   MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV 60

Query: 61  SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY 120
           SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY
Sbjct: 61  SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY 120

Query: 121 MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE 180
           MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE
Sbjct: 121 MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE 180

Query: 181 EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV 240
           EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV
Sbjct: 181 EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV 240

Query: 241 KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI 300
           KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI
Sbjct: 241 KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI 300

Query: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS 360
           FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS
Sbjct: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS 360

Query: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML 420
           SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML
Sbjct: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML 420

Query: 421 DEDEMNILENDQQFLAFASFNPTIILA 448
           DEDEMNILENDQQFLAFASFNPTIILA
Sbjct: 421 DEDEMNILENDQQFLAFASFNPTIILA 447

BLAST of CmaCh14G014900 vs. NCBI nr
Match: XP_022955993.1 (salutaridinol 7-O-acetyltransferase-like [Cucurbita moschata])

HSP 1 Score: 834.3 bits (2154), Expect = 4.8e-238
Identity = 418/447 (93.51%), Postives = 430/447 (96.20%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV 60
           MEVEIISSETIKPSSFPPS +SLKLSV+DQLSPFTYTSL+FFYSLKHSH PLHLSHPLK 
Sbjct: 1   MEVEIISSETIKPSSFPPSLRSLKLSVLDQLSPFTYTSLVFFYSLKHSHVPLHLSHPLKA 60

Query: 61  SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY 120
           SLS ALSDFYLLAGAIKDNTH+LDNGVGALFQVARVH TMP+FLNQPSFESLSQLIPFRY
Sbjct: 61  SLSHALSDFYLLAGAIKDNTHILDNGVGALFQVARVHRTMPEFLNQPSFESLSQLIPFRY 120

Query: 121 MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE 180
           +QTRS STME  YPQVAVQLNAF GGGVAVGVCFLHKIIDGTTLSGFLRRWASMAG STE
Sbjct: 121 VQTRSLSTMEPSYPQVAVQLNAFSGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGVSTE 180

Query: 181 EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV 240
           EKGGGEQPEYG AA FPGRELLV NSWLSKGYSPFVGEGRICRKRFVFDGAAISELK EV
Sbjct: 181 EKGGGEQPEYGTAATFPGRELLVGNSWLSKGYSPFVGEGRICRKRFVFDGAAISELKEEV 240

Query: 241 KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI 300
           KRKSDK+PTSVEVVTGFIWKYMMIAARQS+ S+RSSVLTHAVDLRRRMAPPLPATSIGNI
Sbjct: 241 KRKSDKIPTSVEVVTGFIWKYMMIAARQSSRSRRSSVLTHAVDLRRRMAPPLPATSIGNI 300

Query: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS 360
           FWSAVAHYDTPKAEIELSELVDLVQRSVAEID+GFIREMAGEEGFEGISRWFMRMKELY+
Sbjct: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDEGFIREMAGEEGFEGISRWFMRMKELYT 360

Query: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML 420
           SKPYSYGFTSWRNMGLNEVDFGWGKASWVS AGPENSVLKNVVVL EGILGDG+EAWIML
Sbjct: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSVAGPENSVLKNVVVLKEGILGDGIEAWIML 420

Query: 421 DEDEMNILENDQQFLAFASFNPTIILA 448
           DEDEMNILENDQQFLAFASFNPTIILA
Sbjct: 421 DEDEMNILENDQQFLAFASFNPTIILA 447

BLAST of CmaCh14G014900 vs. NCBI nr
Match: XP_023526907.1 (BAHD acyltransferase BIA1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.8 bits (2150), Expect = 1.4e-237
Identity = 415/446 (93.05%), Postives = 428/446 (95.96%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV 60
           MEVEIISSETIKPSSF PS +SLKLSV+DQLSPFTYTSLLFFYSLKHSHDPLHLSHPLK 
Sbjct: 1   MEVEIISSETIKPSSFSPSLRSLKLSVLDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKA 60

Query: 61  SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY 120
           SLS ALSDFYLLAGAIKDNTH+LDNGVGALFQVA VH TMP+FLNQPSFESLSQLIPFRY
Sbjct: 61  SLSHALSDFYLLAGAIKDNTHILDNGVGALFQVATVHRTMPEFLNQPSFESLSQLIPFRY 120

Query: 121 MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE 180
           MQT+SSS+MEA YPQVAVQLN F GGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE
Sbjct: 121 MQTKSSSSMEASYPQVAVQLNGFSGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE 180

Query: 181 EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV 240
           EKGGGEQPEYG AA FPGRELLV NSWLSKGYSPFVGEGRICRKRFVFDGAAISELK EV
Sbjct: 181 EKGGGEQPEYGAAATFPGRELLVGNSWLSKGYSPFVGEGRICRKRFVFDGAAISELKEEV 240

Query: 241 KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI 300
           KRKSDKVPTSVEVVTGFIWKYMMIAARQS+GSQRSSVLTHAV+LRRRM PPLPATSIGNI
Sbjct: 241 KRKSDKVPTSVEVVTGFIWKYMMIAARQSSGSQRSSVLTHAVNLRRRMVPPLPATSIGNI 300

Query: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS 360
           FWSAVAHYDTPKAEIELSELVDLVQRSVAEID+GF+REMAGEEGFEGISRW MRMKE+Y 
Sbjct: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDEGFVREMAGEEGFEGISRWLMRMKEVYC 360

Query: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML 420
           SKPY+YGFTSWRNMGLNEVDFGWGKASWVS AGPENSVLKNVVVL EGILGDG+EAWIML
Sbjct: 361 SKPYAYGFTSWRNMGLNEVDFGWGKASWVSVAGPENSVLKNVVVLKEGILGDGIEAWIML 420

Query: 421 DEDEMNILENDQQFLAFASFNPTIIL 447
           DEDEMNILENDQQFLAFASFNPTIIL
Sbjct: 421 DEDEMNILENDQQFLAFASFNPTIIL 446

BLAST of CmaCh14G014900 vs. NCBI nr
Match: KAG6582062.1 (Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 818.5 bits (2113), Expect = 2.7e-233
Identity = 413/447 (92.39%), Postives = 425/447 (95.08%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLKV 60
           MEVEIISSE IKPSSFPPS +SLKLSV+DQLSPFTYTSL+FFYSLKHSHDPLHLSHPLK 
Sbjct: 1   MEVEIISSEIIKPSSFPPSLRSLKLSVLDQLSPFTYTSLVFFYSLKHSHDPLHLSHPLKA 60

Query: 61  SLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFRY 120
           SLS ALSDFYLLAGAIKDNTH+LDNGVGALFQVARVH TMP+FLNQ SFESLSQLIPFRY
Sbjct: 61  SLSHALSDFYLLAGAIKDNTHILDNGVGALFQVARVHRTMPEFLNQLSFESLSQLIPFRY 120

Query: 121 MQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGSTE 180
           +QTRS STME      AVQLNAF GGGVAVGVCFLHKIIDGTTLSGFLRRWASMAG STE
Sbjct: 121 VQTRSPSTMETF----AVQLNAFSGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGVSTE 180

Query: 181 EKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKAEV 240
           EKGGGEQPEYG AA FPGRELLV NSWLSKGYSPFVGEGRICRKRFVFDGAAISELK EV
Sbjct: 181 EKGGGEQPEYGAAATFPGRELLVGNSWLSKGYSPFVGEGRICRKRFVFDGAAISELKEEV 240

Query: 241 KRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIGNI 300
           KRKSDK+PTSVEVVTGFIWKYMMIAARQS+GS+RSSVLTHAVDLRRRMAPPL ATSIGNI
Sbjct: 241 KRKSDKIPTSVEVVTGFIWKYMMIAARQSSGSRRSSVLTHAVDLRRRMAPPLQATSIGNI 300

Query: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRMKELYS 360
           FWSAVAHYDTPKAEIELSELVDLVQRSVAEID+GFIREMAGEEGFEGISRWFMRMKELY+
Sbjct: 301 FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDEGFIREMAGEEGFEGISRWFMRMKELYT 360

Query: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEAWIML 420
           SKPYSYGFTSWRNMGLNEVDFGWGKASWVS AGPENSVLKNVVVL EGILGDG+EAWIML
Sbjct: 361 SKPYSYGFTSWRNMGLNEVDFGWGKASWVSVAGPENSVLKNVVVLKEGILGDGIEAWIML 420

Query: 421 DEDEMNILENDQQFLAFASFNPTIILA 448
           DEDEMNILENDQQFLAFASFNPTIILA
Sbjct: 421 DEDEMNILENDQQFLAFASFNPTIILA 443

BLAST of CmaCh14G014900 vs. NCBI nr
Match: XP_038882357.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 672.2 bits (1733), Expect = 3.2e-189
Identity = 354/452 (78.32%), Postives = 389/452 (86.06%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPP--SPKSLKLSVIDQLSPFTYTSLLFFYSLKH-SHDPLHLSHP 60
           MEVEIISSETIKPSSFPP    KSLKLSV+DQLSPFTYTSL+FFY+L + S+DPLHLSH 
Sbjct: 1   MEVEIISSETIKPSSFPPYLHLKSLKLSVLDQLSPFTYTSLVFFYTLNNDSNDPLHLSHA 60

Query: 61  LKVSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIP 120
           LK SLS+ALS+FYLLAG IKD++ +L NGVGALFQ ARVH  M +FLNQPSFESLSQLIP
Sbjct: 61  LKTSLSKALSEFYLLAGIIKDDSQILANGVGALFQFARVHGAMFKFLNQPSFESLSQLIP 120

Query: 121 FRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGG 180
           FR  +  SSSTMEA+YPQVAVQLNAF GGGVAVGVCFLHKIIDGTTLS FLRRWA+ AG 
Sbjct: 121 FRSSRNMSSSTMEAIYPQVAVQLNAFNGGGVAVGVCFLHKIIDGTTLSNFLRRWAAGAGS 180

Query: 181 STEEKGGGEQPEY-GVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISEL 240
           STEE+GGG  PEY    A FPGR+LL  NSWLSKGYSPFVG+G+I R+RFVF G  I +L
Sbjct: 181 STEERGGGRLPEYLSSTAAFPGRDLL-GNSWLSKGYSPFVGKGKIKRRRFVFKGDVILKL 240

Query: 241 KAE-VKRKSDKVPTSVEVVTGFIWKYMMIAA-RQSTGSQRSSVLTHAVDLRRRMAPPLPA 300
           K E VK K+ K PTSVE+VT FIWKYMMIAA ++S+GS R SVLTHAVDLRRRM PPLP 
Sbjct: 241 KEELVKWKNVKNPTSVELVTAFIWKYMMIAAIQKSSGSPRPSVLTHAVDLRRRMVPPLPP 300

Query: 301 TSIGNIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMR 360
           T IGNIFWSAVAHYD+P  EIELS+LV+L+Q+S AEID  FI+EM GEEGF+ IS+WFMR
Sbjct: 301 TIIGNIFWSAVAHYDSPNTEIELSKLVNLIQQSFAEIDYKFIQEMTGEEGFQTISKWFMR 360

Query: 361 MKELYSSKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGL 420
           M+ELYSS PYSYGFTSWRNMGLNEVDFGWGK SWVS AGPENSVLKNVVVL E ILGDG+
Sbjct: 361 MQELYSSNPYSYGFTSWRNMGLNEVDFGWGKPSWVSLAGPENSVLKNVVVLKEAILGDGI 420

Query: 421 EAWIMLDEDEMNILENDQQFLAFASFNPTIIL 447
           EAWIMLDEDEMNILENDQQFLAFAS NPTI L
Sbjct: 421 EAWIMLDEDEMNILENDQQFLAFASNNPTIHL 451

BLAST of CmaCh14G014900 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 230.7 bits (587), Expect = 2.3e-60
Identity = 152/449 (33.85%), Postives = 233/449 (51.89%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPSP-KSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLK 60
           M V+++S + IKPSS  P+  K  KLS+++QL P  +  ++FFYS  +S  P      LK
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQMLK 60

Query: 61  VSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIPFR 120
            SLS  L+ FY LAG +K N  +  N  GA F  ARV+  +   L +PS +SL QLIP  
Sbjct: 61  KSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLIP-- 120

Query: 121 YMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAG-GS 180
                S  ++E     +  Q + F  G +++GVC  HK+ D T++  F++ WA+++  GS
Sbjct: 121 ----TSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGS 180

Query: 181 TEEKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKA 240
            +  G    P +    +FP        +  +    P +   +   KRF+FD ++I  L+A
Sbjct: 181 IKTIGA---PVFDTVKIFPPGNF--SETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQA 240

Query: 241 EVKRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSIG 300
           +        PT VE V+  IWK  M A R  +G+ + S+L ++V LR R++PP    SIG
Sbjct: 241 KASSFEVNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILANSVSLRSRVSPPFTKNSIG 300

Query: 301 NIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGE-EGFEGISRWFMRMKE 360
           N+     A  +    + +L  LV  ++++     D  I ++ G     E I  +     +
Sbjct: 301 NLVSYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGD 360

Query: 361 LYSSKPYS-YGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGLEA 420
           + +S  +  Y F+S    GL E DFGWGK  WV F        KN+V L +     G+EA
Sbjct: 361 MIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQ---KNIVTLLDTKEAGGIEA 420

Query: 421 WIMLDEDEMNILENDQQFLAFASFNPTII 446
           W+ L+E EMN+ E D++ L FAS NP++I
Sbjct: 421 WVNLNEQEMNLFEQDRELLQFASLNPSVI 435

BLAST of CmaCh14G014900 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 199.5 bits (506), Expect = 5.6e-51
Identity = 163/460 (35.43%), Postives = 235/460 (51.09%), Query Frame = 0

Query: 3   VEIISSETIKPSSFPPSPKS-LKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHL------- 62
           VEI+S E +KPSS  P  K  L LS++D LS   YT  L FY+     DP +L       
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAA----DPQNLLGFSTEE 64

Query: 63  -SHPLKVSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHHTMPQFLNQPSFESLS 122
            S  LK SLS+ L  FY LAG I   + V  N  GA+F  ARV H + +FL  P  ESL 
Sbjct: 65  TSLKLKKSLSKTLPIFYPLAGRI-IGSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLE 124

Query: 123 QLIPFRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWAS 182
            LIP       + S     +P + +Q N F  GG+ + +C  HKI D T+L+ F+R WA 
Sbjct: 125 LLIP-----VEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAE 184

Query: 183 MAGGSTEEKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAI 242
            + G     G    P +  + +FP  + L E           V E     KRFVFD + I
Sbjct: 185 SSRG----LGITLIPSFTASEVFP--KPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKI 244

Query: 243 SELKAEVKRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLP 302
            +L+A+  R   K PT VE VT   W+ +   +R S+ + R+SVL   V+LR ++   L 
Sbjct: 245 KKLRAKASRNLVKNPTRVEAVTALFWRCVTKVSRLSSLTPRTSVLQILVNLRGKV-DSLC 304

Query: 303 ATSIGNIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFM 362
             +IGN+    +   +    E  + ++VD ++R+  EI     +EM+     +  SR F 
Sbjct: 305 ENTIGNMLSLMILKNEEAAIE-RIQDVVDEIRRA-KEIFSLNCKEMS-----KSSSRIFE 364

Query: 363 RMKELYSSKPYSYG-------FTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLN 422
            ++E+   K Y  G         SW  +GL + DFGWGK  WV+  G   S  KN+++L 
Sbjct: 365 LLEEI--GKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVT--GRGTSHFKNLMLLI 424

Query: 423 EGILGDGLEAWIMLDEDEMNILENDQQFLAFASFNPTIIL 447
           +   G+G+EAWI L E++M++ E DQ+ L  AS NP +++
Sbjct: 425 DTKDGEGIEAWITLTEEQMSLFECDQELLESASLNPPVLI 436

BLAST of CmaCh14G014900 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-50
Identity = 149/456 (32.68%), Postives = 232/456 (50.88%), Query Frame = 0

Query: 1   MEVEIISSETIKPSSFPPSP-KSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLK 60
           M+VE I+ E IKPSS  P+  ++L+LS+ D + P  YT    FY+      P   SH LK
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSSHKLK 63

Query: 61  VSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARV-HHTMPQFLNQPSFESLSQLIPF 120
            SL+  L+ FY LAG IK  T +  N  GA+F  ARV ++ +  FL  P F++L QL+P 
Sbjct: 64  TSLAETLTKFYPLAGRIKGVT-IDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQLLPL 123

Query: 121 RYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGS 180
             +     +     +P + V+   F  GG+A+G+C  HKI D T++S F++ WA+MA G 
Sbjct: 124 DVIDDPYEAA--TTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMARGE 183

Query: 181 TEEKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICR--KRFVFDGAAISEL 240
             +      P +  A  +P    L +       +       +I +  KRFVF  + + EL
Sbjct: 184 AGDIVA--DPVFAAANFYPPANELFK-------FPVDENANKISQITKRFVFSASKLEEL 243

Query: 241 KAEVKRKSDKV--PTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPA 300
           + +     D V  P  VE VT  +WK  + AA  +T +    VL    +LR ++   L  
Sbjct: 244 RTKA-ASEDFVGRPKRVESVTALLWKAFVAAASSNTNACDMKVLIQPSNLRPKIPSLLAE 303

Query: 301 TSIGNIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMR 360
           + IGNI +S+V    + + EI++ + V  +++   ++ +  I+E  G       S+    
Sbjct: 304 SLIGNIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQN-VIKEEGGSSSAMIGSKLANL 363

Query: 361 MKELYSSKPYS----YGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGIL 420
           M   YS   Y     Y  +SW  + L E  FGWG   W+  AG    +L+NV +L +   
Sbjct: 364 MLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWI--AGNVAPMLENVTMLIDSKD 423

Query: 421 GDGLEAWIMLDEDEMNILENDQQFLAFASFNPTIIL 447
           G G+EA++ L E+ M  LE + + LAFAS NP++++
Sbjct: 424 GQGIEAFVTLPEENMLSLEQNTELLAFASVNPSVLV 443

BLAST of CmaCh14G014900 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 196.4 bits (498), Expect = 4.8e-50
Identity = 145/454 (31.94%), Postives = 227/454 (50.00%), Query Frame = 0

Query: 1   MEVEIISSETIKPSS-FPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPLK 60
           M+VE IS E IKPSS  P + ++L+LS+ D + P  YT    FY+        H SH LK
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHTSHKLK 65

Query: 61  VSLSRALSDFYLLAGAIKDNTHVLDNGVGALFQVARVHH-TMPQFLNQPSFESLSQLIPF 120
            SLS  L+ FY LAG I   T V     GA+F  ARV++  + +FL  P F++L QL+P 
Sbjct: 66  TSLSETLTKFYPLAGRITGVT-VDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQLLPL 125

Query: 121 RYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGGS 180
             +   +     A +P + V+   F  GG+A+G+C  HKI D  ++S F+R WA+ A G 
Sbjct: 126 DVVD--NPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARGE 185

Query: 181 TEEKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELKA 240
             +    E P +  A  +P       N           G+     KRFVF+ + + +L+ 
Sbjct: 186 -NDAAAMESPVFAGANFYP-----PANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRT 245

Query: 241 E-VKRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSI 300
           +    ++   PT VE VT  IWK  + +++ +T   +  VL    +LR ++   L  +SI
Sbjct: 246 KAASEETVDQPTRVESVTALIWKCFVASSKTTTCDHK--VLVQLANLRSKIPSLLQESSI 305

Query: 301 GNIFWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGI-SRWFMRMK 360
           GN+ +S+V        E+++ E V  +++   E+    + E    +    I S+    M 
Sbjct: 306 GNLMFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANLML 365

Query: 361 ELYSSKPYS----YGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGD 420
             YS   Y     Y  +SW  + L E  FGW    WV   G  + VL N+ +L +   G 
Sbjct: 366 TNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWV--VGNVSPVLGNLAMLIDSKDGQ 425

Query: 421 GLEAWIMLDEDEMNILENDQQFLAFASFNPTIIL 447
           G+EA++ L E+ M+  E + + LAFA+ NP++++
Sbjct: 426 GIEAFVTLPEENMSSFEQNPELLAFATMNPSVLV 446

BLAST of CmaCh14G014900 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-46
Identity = 138/453 (30.46%), Postives = 224/453 (49.45%), Query Frame = 0

Query: 1   MEVEIISSETIKPS--SFPPSPKSLKLSVIDQLSPFTYTSLLFFYSLKHSHDPLHLSHPL 60
           +E+ + S E +KPS  +    P    LS +DQL+P  +   LFFY  K +      S  +
Sbjct: 3   LEITVTSQELVKPSPRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKERSDHI 62

Query: 61  KVSLSRALSDFYLLAGAIKDNTH-VLDNGVGALFQVARVHHTMPQFLNQPSFESLSQLIP 120
           K SLS  L+ +Y LAG IK++   V+ N VG  F  A+    M Q L  P+   L++L P
Sbjct: 63  KSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNELNKLHP 122

Query: 121 FRYMQTRSSSTMEALYPQVAVQLNAFVGGGVAVGVCFLHKIIDGTTLSGFLRRWASMAGG 180
           F + +             + VQL  F  GG+A+G+   HK+ D  +   F+  WA+ A G
Sbjct: 123 FEFHEVSD--------VPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARG 182

Query: 181 STEEKGGGEQPEYGVAAMFPGRELLVENSWLSKGYSPFVGEGRICRKRFVFDGAAISELK 240
            T+E      P + +A MFP  +  +EN  ++ G    + +  I  +RFVF  +++  L+
Sbjct: 183 QTDEI---ITPSFDLAKMFPPCD--IENLNMATG----ITKENIVTRRFVFLRSSVESLR 242

Query: 241 AEVKRKSDKVPTSVEVVTGFIWKYMMIAARQSTGSQRSSVLTHAVDLRRRMAPPLPATSI 300
                      T VEV++ FIW   M +      + +   L H V+LRR+  P +P    
Sbjct: 243 ERFSGNKKIRATRVEVLSVFIWSRFMASTNHDDKTGKIYTLIHPVNLRRQADPDIPDNMF 302

Query: 301 GNI--FWSAVAHYDTPKAEIELSELVDLVQRSVAEIDDGFIREMAGEEGFEGISRWFMRM 360
           GNI  F   V      + + E + LVD ++  + +ID  +++++  +E   G   +  + 
Sbjct: 303 GNIMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKL--QEDNRGHLEFLNKQ 362

Query: 361 KE-LYSSKPYSYGFTSWRNMGLNEVDFGWGKASWVSFAGPENSVLKNVVVLNEGILGDGL 420
                + +  S+ FTS     + E DFGWGK  WV+ A       KN+V   +   GDG+
Sbjct: 363 ASGFVNGEIVSFSFTSLCKFPVYEADFGWGKPLWVASA---RMSYKNLVAFIDTKEGDGI 422

Query: 421 EAWIMLDEDEMNILENDQQFLAFASFNPTIILA 448
           EAWI LD+++M+  E D++ L + S NP+++++
Sbjct: 423 EAWINLDQNDMSRFEADEELLRYVSSNPSVMVS 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A2P1GIW74.3e-4832.80Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q94FT47.7e-4532.98Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 ... [more]
O233931.4e-4331.33BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1[more]
Q9FI402.5e-4330.74BAHD acyltransferase At5g47980 OS=Arabidopsis thaliana OX=3702 GN=BAHD1 PE=2 SV=... [more]
Q70PR71.1e-4032.82Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1IPN43.6e-255100.00BAHD acyltransferase BIA1-like OS=Cucurbita maxima OX=3661 GN=LOC111479388 PE=3 ... [more]
A0A6J1GVJ32.3e-23893.51salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A5A7TN591.4e-17975.22Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3BWZ01.4e-17975.22vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494520 PE=3 SV=1[more]
A0A0A0KZ245.5e-17974.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016450 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022979792.17.4e-255100.00BAHD acyltransferase BIA1-like [Cucurbita maxima][more]
XP_022955993.14.8e-23893.51salutaridinol 7-O-acetyltransferase-like [Cucurbita moschata][more]
XP_023526907.11.4e-23793.05BAHD acyltransferase BIA1-like [Cucurbita pepo subsp. pepo][more]
KAG6582062.12.7e-23392.39Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_038882357.13.2e-18978.32stemmadenine O-acetyltransferase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G26040.12.3e-6033.85HXXXD-type acyl-transferase family protein [more]
AT1G24420.15.6e-5135.43HXXXD-type acyl-transferase family protein [more]
AT3G30280.11.6e-5032.68HXXXD-type acyl-transferase family protein [more]
AT4G15390.14.8e-5031.94HXXXD-type acyl-transferase family protein [more]
AT1G24430.11.1e-4630.46HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 1..434
e-value: 6.2E-70
score: 236.0
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 220..438
e-value: 9.9E-56
score: 190.2
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..202
e-value: 2.4E-46
score: 159.7
NoneNo IPR availablePANTHERPTHR31623:SF36VINORINE SYNTHASE-LIKEcoord: 3..444
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 3..444

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G014900.1CmaCh14G014900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups