CmaCh14G010000 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G010000
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Ribonuclease T(2)
Location	Cma_Chr14: 5244722 .. 5245385 (+)
RNA-Seq Expression	CmaCh14G010000
Synteny	CmaCh14G010000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATCTCAACCTTTCGACGACTTCTGGTTTGTTCAACAATGGCCACCCAATGTTTGCGCTTTACAATTAGGACGATGTGTAGGACGAGGGACAAATTCCTTCACCATCCATGGTTTGTGGCCTCAGAAGAGAGGAAATTCTGTAACTAATTGTCCCGGTACCTCCTTTGATTTTAATCAGGTAAAACTCAAACATGTATTTATTCTAGTTCAATTTATTAGATGTGCAAATTATAAAGTTCATTATGTTTGGGTTTCAGATTGCGCATTTAGAAAACGATCTTAACGTTGTTTGGCCAAATCTGATCACTGGAAACCACAAATGGTTTTGGGGTCATGAGTGGAACACACATGGGATTTGCTCAGAAAGTAGGTTCGATGAGGCAGCCTACTTCCAAACATCCATAAATTTGAGGCACAATATTGACCTCCTTAGCGCCCTAAGAGTTCAAGGAGTAGTTCCAAATGGGGCCTCCAAGTCGAAGCAAAGAGTGGAAAACGCCATTATAAAGCATTTCAAGAATAATCCTGTTCTTCGATGTAAAACAGCATCGAACGGCCAAGTCCTATTGACGGAGATTGTTATGTGCATCGATGACGATGGTGTCACCCTTATCAATTGCAATCTTGCAAAATCCAATTGCGCTAACAGCTTCATTTTCTAA

mRNA sequence

AAATCTCAACCTTTCGACGACTTCTGGTTTGTTCAACAATGGCCACCCAATGTTTGCGCTTTACAATTAGGACGATGTGTAGGACGAGGGACAAATTCCTTCACCATCCATGGTTTGTGGCCTCAGAAGAGAGGAAATTCTGTAACTAATTGTCCCGGTACCTCCTTTGATTTTAATCAGATTGCGCATTTAGAAAACGATCTTAACGTTGTTTGGCCAAATCTGATCACTGGAAACCACAAATGGTTTTGGGGTCATGAGTGGAACACACATGGGATTTGCTCAGAAAGTAGGTTCGATGAGGCAGCCTACTTCCAAACATCCATAAATTTGAGGCACAATATTGACCTCCTTAGCGCCCTAAGAGTTCAAGGAGTAGTTCCAAATGGGGCCTCCAAGTCGAAGCAAAGAGTGGAAAACGCCATTATAAAGCATTTCAAGAATAATCCTGTTCTTCGATGTAAAACAGCATCGAACGGCCAAGTCCTATTGACGGAGATTGTTATGTGCATCGATGACGATGGTGTCACCCTTATCAATTGCAATCTTGCAAAATCCAATTGCGCTAACAGCTTCATTTTCTAA

Coding sequence (CDS)

AAATCTCAACCTTTCGACGACTTCTGGTTTGTTCAACAATGGCCACCCAATGTTTGCGCTTTACAATTAGGACGATGTGTAGGACGAGGGACAAATTCCTTCACCATCCATGGTTTGTGGCCTCAGAAGAGAGGAAATTCTGTAACTAATTGTCCCGGTACCTCCTTTGATTTTAATCAGATTGCGCATTTAGAAAACGATCTTAACGTTGTTTGGCCAAATCTGATCACTGGAAACCACAAATGGTTTTGGGGTCATGAGTGGAACACACATGGGATTTGCTCAGAAAGTAGGTTCGATGAGGCAGCCTACTTCCAAACATCCATAAATTTGAGGCACAATATTGACCTCCTTAGCGCCCTAAGAGTTCAAGGAGTAGTTCCAAATGGGGCCTCCAAGTCGAAGCAAAGAGTGGAAAACGCCATTATAAAGCATTTCAAGAATAATCCTGTTCTTCGATGTAAAACAGCATCGAACGGCCAAGTCCTATTGACGGAGATTGTTATGTGCATCGATGACGATGGTGTCACCCTTATCAATTGCAATCTTGCAAAATCCAATTGCGCTAACAGCTTCATTTTCTAA

Protein sequence

KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLINCNLAKSNCANSFIF

Homology

[Close]

BLAST of CmaCh14G010000 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.3e-52
Identity = 93/193 (48.19%), Postives = 128/193 (66.32%), Query Frame = 0


Query: 5   FDDFWFVQQWPPNVCALQ-LGRCVGRGTNSFTIHGLWPQKRGNSVTNCP-GTSFDFNQIA 64
           FD FWFVQQWPP VC+ Q  G C G G  +FTIHGLWPQ  G S+TNCP G+ FD  +I+
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 65  HLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSALR 124
           HL++ LN +WPN++  N++ FW HEW  HG CSES F++AAYF+ ++++R+N D++ ALR
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 125 VQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQV-LLTEIVMCIDDDGVTLINC 184
                PNG +KS+Q ++  +   F   P LRC+T    +V  L ++V C   DG TLI+C
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

Query: 185 NLAKSNCANSFIF 195
              +  C  +FIF
Sbjct: 181 --TRDTCGANFIF 191

[Close]

BLAST of CmaCh14G010000 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.1e-31
Identity = 71/187 (37.97%), Postives = 101/187 (54.01%), Query Frame = 0


Query: 2   SQPFDDFWFVQQWPPNVCALQLGRCV---GRGTNSFTIHGLWPQ-KRGNSVTNCPGTS-F 61
           S+ FD F+FVQQWP + C  Q   C    G+    F IHGLWP  K G   +NC  +  F
Sbjct: 27  SEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPF 86

Query: 62  DFNQIAHLENDLNVVWPNLI--TGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHN 121
           D + I+ L   +   WP L   +G+ + FW HEW  HG CSES  D+  YFQT++NL+  
Sbjct: 87  DSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQK 146

Query: 122 IDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDD 181
            +LL AL   G+ P+G S S + + ++I +     P + C    +G   L ++ +C+D  
Sbjct: 147 TNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

[Close]

BLAST of CmaCh14G010000 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-29
Identity = 70/202 (34.65%), Postives = 107/202 (52.97%), Query Frame = 0


Query: 2   SQPFDDFWFVQQWPPNVCALQLGRC---VGRGTNSFTIHGLWPQKR-GNSVTNC-PGTSF 61
           ++ FD F+FVQQWP + C  +   C    G+    F IHGLWP    G   +NC P + +
Sbjct: 26  AKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNNNDGTYPSNCDPNSPY 85

Query: 62  DFNQIAHLENDLNVVWPNLI--TGNHKWFWGHEWNTHGICSESRF-DEAAYFQTSINLRH 121
           D +QI+ L + +   WP L   +G+   FW HEW  HG C+ES   ++ AYF+ +++L++
Sbjct: 86  DQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVLTNQHAYFKKALDLKN 145

Query: 122 NIDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDD 181
            IDLLS L+   + P+G S     + NAI       P ++C    +G   L ++ +C+D 
Sbjct: 146 QIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNVDQSGNSQLYQVYICVDG 205

Query: 182 DGVTLINCNL-AKSNCANSFIF 195
            G +LI C +     C  S  F
Sbjct: 206 SGSSLIECPIFPGGKCGTSIEF 227

[Close]

BLAST of CmaCh14G010000 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 7.7e-26
Identity = 65/187 (34.76%), Postives = 92/187 (49.20%), Query Frame = 0


Query: 2   SQPFDDFWFVQQWPPNVCALQLGRC---VGRGTNSFTIHGLWPQ-KRGNSVTNC-PGTSF 61
           +Q FD F+FV QWP   C  +   C    G+    F IHGLWP  K G    NC P + F
Sbjct: 19  AQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRF 78

Query: 62  DFNQIAHLENDLNVVWPNLI--TGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHN 121
           D  +++ L +DL   WP L   + +   FW HEW  HG C+ES  D+  YF+  + L+  
Sbjct: 79  DDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQK 138

Query: 122 IDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDD 181
            +LL AL   G+ P+      + +EN I +     P + C   S+    L +I +C+D  
Sbjct: 139 ANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTS 198

[Close]

BLAST of CmaCh14G010000 vs. ExPASy Swiss-Prot
Match: Q40875 (Ribonuclease S-3 OS=Petunia hybrida OX=4102 GN=S3 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 8.3e-20
Identity = 58/178 (32.58%), Postives = 89/178 (50.00%), Query Frame = 0


Query: 2   SQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQI 61
           S  FD F  V  WP + C     +C  R +N+FTIHGLWP+K+   +  C G    + + 
Sbjct: 21  SANFDYFQLVLTWPASFC-YPKNKC-QRRSNNFTIHGLWPEKKRFRLEFCTGDK--YKRF 80

Query: 62  AHLENDLNVV--------WPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRH 121
              +N +NV+        +        +  W HE+N HGIC ++ +D+ AYF  ++ L+ 
Sbjct: 81  LEEDNIINVLERHWIQMRFDETYANTKQPLWEHEYNRHGICCKNLYDQKAYFLLAMRLKD 140

Query: 122 NIDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNN-PVLRCKTASNGQVLLTEIVMC 171
            +DLL+ LR  G+ P G   +   ++ AI     NN P L+C     G + L EI +C
Sbjct: 141 KLDLLTTLRTHGITP-GTKHTFGEIQKAIKTVTSNNDPDLKCVENIKGVMELNEIGIC 193

[Close]

BLAST of CmaCh14G010000 vs. ExPASy TrEMBL
Match: A0A6J1J1Y7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 7.1e-115
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 60
           KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ
Sbjct: 22  KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 81

Query: 61  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 120
           IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA
Sbjct: 82  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 141

Query: 121 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 180
           LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN
Sbjct: 142 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 201

Query: 181 CNLAKSNCANSFIF 195
           CNLAKSNCANSFIF
Sbjct: 202 CNLAKSNCANSFIF 215

[Close]

BLAST of CmaCh14G010000 vs. ExPASy TrEMBL
Match: A0A6J1F776 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 5.1e-113
Identity = 191/194 (98.45%), Postives = 192/194 (98.97%), Query Frame = 0


Query: 1   KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 60
           KSQPFDDFWFVQQWPPNVCALQ GRCVGRGT SFTIHGLWPQKRGNSVTNCPGTSFDFNQ
Sbjct: 22  KSQPFDDFWFVQQWPPNVCALQSGRCVGRGTKSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 81

Query: 61  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 120
           IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA
Sbjct: 82  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 141

Query: 121 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 180
           LRVQGVVPNGASKSKQRVENAIIKHF+NNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN
Sbjct: 142 LRVQGVVPNGASKSKQRVENAIIKHFQNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 201

Query: 181 CNLAKSNCANSFIF 195
           CNLAKSNCANSFIF
Sbjct: 202 CNLAKSNCANSFIF 215

[Close]

BLAST of CmaCh14G010000 vs. ExPASy TrEMBL
Match: A0A6J1FBD8 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111442477 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.4e-107
Identity = 180/194 (92.78%), Postives = 188/194 (96.91%), Query Frame = 0


Query: 1   KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 60
           KSQPFDDFWFVQQWPPNVCALQ GRCVGRGTNSFTIHGLWPQK GNSVTNCPGTSFDFNQ
Sbjct: 22  KSQPFDDFWFVQQWPPNVCALQSGRCVGRGTNSFTIHGLWPQKGGNSVTNCPGTSFDFNQ 81

Query: 61  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 120
           I+HLENDLN+VWPNLITGNHKWFWGHEWNTHGICSES+FD+ AYFQTSINLRHNI+LLSA
Sbjct: 82  ISHLENDLNIVWPNLITGNHKWFWGHEWNTHGICSESKFDQKAYFQTSINLRHNINLLSA 141

Query: 121 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 180
           LRVQGVVPNGASKSKQRVE+AII HFK  PVLRCKTASNGQVLLTEIVMCIDDDGVTLIN
Sbjct: 142 LRVQGVVPNGASKSKQRVESAIISHFKKIPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 201

Query: 181 CNLAKSNCANSFIF 195
           CN+AKSNCAN+FIF
Sbjct: 202 CNIAKSNCANNFIF 215

[Close]

BLAST of CmaCh14G010000 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.2e-91
Identity = 155/194 (79.90%), Postives = 167/194 (86.08%), Query Frame = 0


Query: 1   KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 60
           KSQ FDDFWFVQQWPP VC LQ GRCVGRGT SFTIHGLWPQK G SVTNC G  FDF +
Sbjct: 24  KSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKGGRSVTNCTGNQFDFTK 83

Query: 61  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 120
           IAHLENDLNVVWPN++TGN+K+FWGHEWN HGICSES+FDEA YFQT+IN+RH IDLLS 
Sbjct: 84  IAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQTAINMRHGIDLLSV 143

Query: 121 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 180
           LR  GV PNGASK+KQRVE AI  HF  +P+LRCK ASNGQVLLTEIVMC DDDGVTLIN
Sbjct: 144 LRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTEIVMCFDDDGVTLIN 203

Query: 181 CNLAKSNCANSFIF 195
           CN A+SNCA SFIF
Sbjct: 204 CNKARSNCAGSFIF 217

[Close]

BLAST of CmaCh14G010000 vs. ExPASy TrEMBL
Match: A0A1S3B8Q3 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.2e-91
Identity = 152/194 (78.35%), Postives = 171/194 (88.14%), Query Frame = 0


Query: 1   KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 60
           KSQ FD+FWFVQQWPP +C LQ GRCVGRGT SFTIHGLWPQK G SVTNC G SFDF +
Sbjct: 23  KSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIHGLWPQKGGRSVTNCSGNSFDFTK 82

Query: 61  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 120
           IAHLE+DLNVVWPN++TGN+K+FWGHEWN HGICSES+FD+A YFQT+IN+RH+IDLLS 
Sbjct: 83  IAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDQAKYFQTAINMRHSIDLLSV 142

Query: 121 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 180
           LR  G+VPNGASK+KQRVE AI  HF  +P+LRCKTASNGQVLLTEIV+C DDDGVTLIN
Sbjct: 143 LRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTASNGQVLLTEIVICFDDDGVTLIN 202

Query: 181 CNLAKSNCANSFIF 195
           CN A+SNCA SFIF
Sbjct: 203 CNKARSNCARSFIF 216

[Close]

BLAST of CmaCh14G010000 vs. NCBI nr
Match: XP_022983351.1 (ribonuclease MC-like [Cucurbita maxima])

HSP 1 Score: 422.9 bits (1086), Expect = 1.5e-114
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 60
           KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ
Sbjct: 22  KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 81

Query: 61  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 120
           IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA
Sbjct: 82  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 141

Query: 121 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 180
           LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN
Sbjct: 142 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 201

Query: 181 CNLAKSNCANSFIF 195
           CNLAKSNCANSFIF
Sbjct: 202 CNLAKSNCANSFIF 215

[Close]

BLAST of CmaCh14G010000 vs. NCBI nr
Match: XP_022934270.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 416.8 bits (1070), Expect = 1.0e-112
Identity = 191/194 (98.45%), Postives = 192/194 (98.97%), Query Frame = 0


Query: 1   KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 60
           KSQPFDDFWFVQQWPPNVCALQ GRCVGRGT SFTIHGLWPQKRGNSVTNCPGTSFDFNQ
Sbjct: 22  KSQPFDDFWFVQQWPPNVCALQSGRCVGRGTKSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 81

Query: 61  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 120
           IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA
Sbjct: 82  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 141

Query: 121 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 180
           LRVQGVVPNGASKSKQRVENAIIKHF+NNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN
Sbjct: 142 LRVQGVVPNGASKSKQRVENAIIKHFQNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 201

Query: 181 CNLAKSNCANSFIF 195
           CNLAKSNCANSFIF
Sbjct: 202 CNLAKSNCANSFIF 215

[Close]

BLAST of CmaCh14G010000 vs. NCBI nr
Match: XP_023528964.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 403.7 bits (1036), Expect = 9.1e-109
Identity = 182/194 (93.81%), Postives = 189/194 (97.42%), Query Frame = 0


Query: 1   KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 60
           KSQPFDDFWFVQQWPPNVCALQ GRC+GRGTNSFTIHGLWPQK GNSVTNCPGTSFDFNQ
Sbjct: 22  KSQPFDDFWFVQQWPPNVCALQSGRCIGRGTNSFTIHGLWPQKGGNSVTNCPGTSFDFNQ 81

Query: 61  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 120
           I+HLENDLN+VWPNLITGNHKWFWGHEWNTHGICSES++DE AYFQTSINLRHNI+LLSA
Sbjct: 82  ISHLENDLNIVWPNLITGNHKWFWGHEWNTHGICSESKYDEKAYFQTSINLRHNINLLSA 141

Query: 121 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 180
           LRVQGVVPNGASKSKQRVE+AII HFK NPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN
Sbjct: 142 LRVQGVVPNGASKSKQRVESAIISHFKKNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 201

Query: 181 CNLAKSNCANSFIF 195
           CNLAKSNCANSFIF
Sbjct: 202 CNLAKSNCANSFIF 215

[Close]

BLAST of CmaCh14G010000 vs. NCBI nr
Match: XP_022935695.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 398.7 bits (1023), Expect = 2.9e-107
Identity = 180/194 (92.78%), Postives = 188/194 (96.91%), Query Frame = 0


Query: 1   KSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQ 60
           KSQPFDDFWFVQQWPPNVCALQ GRCVGRGTNSFTIHGLWPQK GNSVTNCPGTSFDFNQ
Sbjct: 22  KSQPFDDFWFVQQWPPNVCALQSGRCVGRGTNSFTIHGLWPQKGGNSVTNCPGTSFDFNQ 81

Query: 61  IAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSA 120
           I+HLENDLN+VWPNLITGNHKWFWGHEWNTHGICSES+FD+ AYFQTSINLRHNI+LLSA
Sbjct: 82  ISHLENDLNIVWPNLITGNHKWFWGHEWNTHGICSESKFDQKAYFQTSINLRHNINLLSA 141

Query: 121 LRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 180
           LRVQGVVPNGASKSKQRVE+AII HFK  PVLRCKTASNGQVLLTEIVMCIDDDGVTLIN
Sbjct: 142 LRVQGVVPNGASKSKQRVESAIISHFKKIPVLRCKTASNGQVLLTEIVMCIDDDGVTLIN 201

Query: 181 CNLAKSNCANSFIF 195
           CN+AKSNCAN+FIF
Sbjct: 202 CNIAKSNCANNFIF 215

[Close]

BLAST of CmaCh14G010000 vs. NCBI nr
Match: XP_023529156.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 378.6 bits (971), Expect = 3.2e-101
Identity = 172/178 (96.63%), Postives = 175/178 (98.31%), Query Frame = 0


Query: 7   DFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGNSVTNCPGTSFDFNQIAHLEN 66
           +FWFVQQWPPNVCALQ GRCVGRGT SFTIHGLWPQKRGNSVTNCPGTSFDFNQIAHLEN
Sbjct: 43  NFWFVQQWPPNVCALQSGRCVGRGTKSFTIHGLWPQKRGNSVTNCPGTSFDFNQIAHLEN 102

Query: 67  DLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSALRVQGV 126
           DLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSALRVQGV
Sbjct: 103 DLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNIDLLSALRVQGV 162

Query: 127 VPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDGVTLINCNLA 185
           VPNGASKSKQRVENAI+KHFKNNPVLRCKTAS GQVLLTEIVMCIDDDGVTLINCNL+
Sbjct: 163 VPNGASKSKQRVENAIVKHFKNNPVLRCKTASTGQVLLTEIVMCIDDDGVTLINCNLS 220

[Close]

BLAST of CmaCh14G010000 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 137.1 bits (344), Expect = 1.5e-32
Identity = 71/187 (37.97%), Postives = 101/187 (54.01%), Query Frame = 0


Query: 2   SQPFDDFWFVQQWPPNVCALQLGRCV---GRGTNSFTIHGLWPQ-KRGNSVTNCPGTS-F 61
           S+ FD F+FVQQWP + C  Q   C    G+    F IHGLWP  K G   +NC  +  F
Sbjct: 27  SEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPF 86

Query: 62  DFNQIAHLENDLNVVWPNLI--TGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHN 121
           D + I+ L   +   WP L   +G+ + FW HEW  HG CSES  D+  YFQT++NL+  
Sbjct: 87  DSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQK 146

Query: 122 IDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDD 181
            +LL AL   G+ P+G S S + + ++I +     P + C    +G   L ++ +C+D  
Sbjct: 147 TNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

[Close]

BLAST of CmaCh14G010000 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-28
Identity = 67/200 (33.50%), Postives = 97/200 (48.50%), Query Frame = 0


Query: 3   QPFDDFWFVQQWPPNVCALQLGRCV---GRGTNSFTIHGLWPQKRGNS-VTNC-PGTSFD 62
           + FD F+FV QWP   C      C    G+    F IHGLWP   G S  +NC P + FD
Sbjct: 26  EDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQFD 85

Query: 63  FNQIAHLENDLNVVWPNLITGNHKW--FWGHEWNTHGICSESRFDEAAYFQTSINLRHNI 122
            +QI+ L + L   WP L   +++   FW HEW  HG CSES  D+  YF+ ++ L+   
Sbjct: 86  RSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKLKQKA 145

Query: 123 DLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDG 182
           +LL  L+  G+ P+    +  ++ NAI       P + C         L +I +C+D  G
Sbjct: 146 NLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNAQLHQIYICVDTSG 205

Query: 183 VTLINCN-LAKSNCANSFIF 195
              I C  L + +C +   F
Sbjct: 206 TEFIECPVLPRGSCPSQIQF 225

[Close]

BLAST of CmaCh14G010000 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 118.6 bits (296), Expect = 5.5e-27
Identity = 65/187 (34.76%), Postives = 92/187 (49.20%), Query Frame = 0


Query: 2   SQPFDDFWFVQQWPPNVCALQLGRC---VGRGTNSFTIHGLWPQ-KRGNSVTNC-PGTSF 61
           +Q FD F+FV QWP   C  +   C    G+    F IHGLWP  K G    NC P + F
Sbjct: 19  AQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRF 78

Query: 62  DFNQIAHLENDLNVVWPNLI--TGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHN 121
           D  +++ L +DL   WP L   + +   FW HEW  HG C+ES  D+  YF+  + L+  
Sbjct: 79  DDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQK 138

Query: 122 IDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDD 181
            +LL AL   G+ P+      + +EN I +     P + C   S+    L +I +C+D  
Sbjct: 139 ANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTS 198

[Close]

BLAST of CmaCh14G010000 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 112.5 bits (280), Expect = 3.9e-25
Identity = 63/200 (31.50%), Postives = 95/200 (47.50%), Query Frame = 0


Query: 5   FDDFWFVQQWPPNVCALQLGRC---VGRGTNSFTIHGLWPQ-KRGNSVTNCPGTS-FDFN 64
           F+ F++V  WP  +C  Q G C    G   + F IHGLWPQ   G     C  T+ FD +
Sbjct: 26  FNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLWPQFNNGTWPAFCDQTNLFDIS 85

Query: 65  QIAHLENDLNVVWPN----LITGNHKWFWGHEWNTHGICSESRFDEAAYFQTSINLRHNI 124
           +I+ L   +   W          N    W HEWN HG C +S FD+ +YF+T++  +H +
Sbjct: 86  KISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTCVQSIFDQHSYFRTNLKFKHKV 145

Query: 125 DLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEIVMCIDDDG 184
            LL+ L  +G+ PN    S   ++NAI       P + C     G   L +I +C+D+  
Sbjct: 146 HLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIECNEDVKGNKQLFQIYICLDNYA 205

Query: 185 VTLINC-NLAKSNCANSFIF 195
              + C  +   +CA+   F
Sbjct: 206 KEFVECPYVPDKSCASKIKF 225

[Close]

BLAST of CmaCh14G010000 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 79.0 bits (193), Expect = 4.8e-15
Identity = 61/187 (32.62%), Postives = 80/187 (42.78%), Query Frame = 0


Query: 5   FDDFWFVQQWPPNVCALQLGRC----VGRGTNS---FTIHGLWPQKRGNSVTNCPGTS-F 64
           FD F    QWP   C      C      RG+++   FTIHGLWP     S  +C   S F
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 65  DFNQIAHLENDLNVVWPNL-------ITGNHKWFWGHEWNTHGICSESRF-DEAAYFQTS 124
              +I+ L + L   WP+L         G    FWGHEW  HG CS   F DE  YF T+
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTT 151

Query: 125 INLRHNIDLLSALRVQGVVPNGASK-SKQRVENAIIKHFKNNPVLRCKTASNGQVLLTEI 175
           +NL    ++   L   G V + + K     +  AI   F   P + CK  +     + EI
Sbjct: 152 LNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDA-----IDEI 211

The following BLAST results are available for this feature:

BLAST of CmaCh14G010000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	1.3e-52	48.19	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	2.1e-31	37.97	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80022	1.1e-29	34.65	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42815	7.7e-26	34.76	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
Q40875	8.3e-20	32.58	Ribonuclease S-3 OS=Petunia hybrida OX=4102 GN=S3 PE=3 SV=1	[more]

BLAST of CmaCh14G010000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J1Y7	7.1e-115	100.00	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1	[more]
A0A6J1F776	5.1e-113	98.45	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1	[more]
A0A6J1FBD8	1.4e-107	92.78	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111442477 PE=3 SV=1	[more]
A0A0A0LC37	3.2e-91	79.90	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]
A0A1S3B8Q3	3.2e-91	78.35	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1	[more]

BLAST of CmaCh14G010000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022983351.1	1.5e-114	100.00	ribonuclease MC-like [Cucurbita maxima]	[more]
XP_022934270.1	1.0e-112	98.45	ribonuclease MC-like [Cucurbita moschata]	[more]
XP_023528964.1	9.1e-109	93.81	ribonuclease MC-like [Cucurbita pepo subsp. pepo]	[more]
XP_022935695.1	2.9e-107	92.78	ribonuclease MC-like [Cucurbita moschata]	[more]
XP_023529156.1	3.2e-101	96.63	ribonuclease MC-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh14G010000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	1.5e-32	37.97	ribonuclease 1	[more]
AT1G14220.1	2.9e-28	33.50	Ribonuclease T2 family protein	[more]
AT1G26820.1	5.5e-27	34.76	ribonuclease 3	[more]
AT1G14210.1	3.9e-25	31.50	Ribonuclease T2 family protein	[more]
AT2G39780.1	4.8e-15	32.62	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 5..193 e-value: 3.7E-54 score: 185.6
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 5..194
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 6..181 e-value: 6.2E-43 score: 147.1
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 5..191
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 5..191
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 34..41
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 83..94
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 5..193 e-value: 2.87863E-69 score: 207.57

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G010000.1	CmaCh14G010000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding