CmaCh14G009130 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G009130
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionbifunctional epoxide hydrolase 2-like
LocationCma_Chr14: 4738903 .. 4741840 (+)
RNA-Seq ExpressionCmaCh14G009130
SyntenyCmaCh14G009130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCACGTTATTCACGAGGCCGTTATTTCTCCGGCGAAAAGGATCTTAATACATTCAACGCCGATCTATTTATAGTTCTCTTAAAGAAATTGATTGAAAGTGAACCAGAGAGAGCAACTGCCATGGACGCCATTGAACACACCACCGTCAACACCAATGGCATCAAAATGCACATTGCCTCCATAGGCACAGGCCCCGTCGTTCTTCTTCTCCACGGCTTTCCCGAGCTCTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCTTCCGTCGGATATCGAGCCATTGCACCGGACCTCCGCGGCTATGGCGATACCGACTCTCCAGAATCGCATACTTCCTACACTGCGCTCCATATCGTCGGTGATTTGGTTGGGGCTTTGGACGAGCTTGGTATTGAGAAGGTGTTTCTTGTAGGGCACGATTGGGGGGCGATCATCGCGTGGTACTTTTGCCTGTTTAGACCTGATAGAATCAAGGCCTTGGTGAATCTGAGTGTTCAGTTCTTCCCTAGAAATCCGAAAATTTCTTTCATCGAACGATTCAGAGCTGTGTATGGTGATGATTTCTATATGTGCAGGTTTCAGGTGAATCTCAACTTTGATTGACTTCTAAGAAGAGATCTAATGGGTTTTATTCCGGTCTGATTTTGGTCCTAATTTCGATTAATCTCTGTGTTTATCTTAGTTGCTTGTAGTTGGATATATGGTGTAATCAATTTGTTTTCTACTACTGATGCTCATAGCTGTAGTGTTTTAGTGTTCTGGTGTAGATTTGATCTGTGCACTCACTCACATACACAATTGAAACCCTAATTATGTGAAATCACTGTACTGTTGAAGAATCAAACCTTAAAATATTGCTTTTCTTAACATATTCAAAAGTTCTATACAGCTTTTAGACTGTTTCATCTTTGACAAAATTAATTTCAGATCTACAAGCTGTTAAATCTTACTAATTTTTTCTTCTCTTCTGCAAGTGATTGCAAACTTTCCTGGAACAGAGTTTTGTAGAAGTTGTAGTTGACAGTCGAGTACCCTATTGGAACATTCCTTTTGACCTTTTTGTGATTCTCTCTTGATTCATTAAGTTGCAGATTAGAGAGAGTCCCATCTGAATTTTGATTCATCATCTGTTTGATTCATCAAATTTTGAGAGCCATATCGAACTTTGATATCAAAATAAAGTCTGTTCTACTGATAGACCATGTTTTATTAACTTATAGAGCAATTTCTGATATCATTTTTGTTGGAAACATGGGGATGGCTCATGCAAGCAGGAGAGATACAAATGAATTGATTGTGGTTCTATAATACATTCATGGCATCTAAGACAACTAAGTGTAGATTGATTTGTAACAGGTTCCAGGGGAGGCAGAAGCAGATTTTTTCTGCATCGACACAGCTCAACTCTTCAAGTCAACACTATCTAATAGAAGTACTCATGCTCCATGCTTGCCTAAAGAGTACGGATTTCGCGCGATCCCGCCGCCAGAAAACCTTCCATCTTGGTTGACGGAAGAAGATATCAACCATTATGCTTCCAAATTTAAGGAGACGGGCTTCACTGGAGCATTGAACTACTATCGCGCTTTTGACCTGTAAGCCATGTTTATTTACAACCCAAGTTCTCTGTTAATACCATTCCAATCCTACACTCTTCTCTGCGTTCTAAGTTTAACAATGGTTTCGTGTTTCGACATTAAATACATGTTCTGTTTAATTCTGATGCATTTTCATGGTGAGAAACTGTCGAATTACACAACCTTAGATTTTTACTTAACCTAAAGTCGCTACGTTATATGTATATGTATATTATGTATATATATGGTTCCCACATCGGTTGAAGAGAAGAACGGAGAATTCTCTATAAGAGTGTGTAAACTTCTCCTTAATAGACATGTTTTGAAAATCTTGAAGGGAAGTTTGAAAGGAAAAGCCCAAAAAGGACAATAATTGTTGGTTAGACTGTTACAAATGGTATTAGAGTCTCGAGTGATGTACTAGCAAGGAGACTGAGCCCCAAAGGGGGTAGACACTAGACAATGTGCATGCGAGAACATTGGGCCCCGAAGAGGGGTGGATTGGGGGTCCCACATCGATTGTAGAGGGAAAAAGTGTAAGCAAGTGGATTGGGGGTCCTACATCGATTGTAGAGGGAAAAAGTGTAAGCAAGTATGCTGGCCCCGAAGAGGTGCTTGTGAGATCCCACATTGGTTGAAGAAGGAACAAAGTATTCTTTATAACGGTGTGGAATCCTCTTTCTTACCAGACACGTTTTAAAAACCTTGAGGGAAAGCACTAAAGTTAAAACCCTATATAGGACAATATCTGCTAGCGAAACCTCGAGATAAAGCCCAAAAGGACAATATCTATTAGCGGTGAGCTTAAACGGTTACAATTTGACTTTATTTTGCTCGCTTATAAATGAAATACATATCGTTAGCTATAGGTTGTTGAAGGCAGCGGATAACGTTGATGGTTGATTATGATATATGAGTTGAATTGATGTTTATTGCAGAACCTGGGAGCTGACGGGGCCATGGACGGGAGCACAGATTCAGGTACCTGTGAAATTCATCGTTGGAGATTTGGATATAACGTATCATTTTGAAGGAGCCAAGGAATATATCCATAATGGGGGATTCAAAAGGGACGTGCCATTGCTGGAGGAAGTTGTTATTGTAGAAGATGCTGGTCACTTTGTACACGAAGAAAGGCCTCATGAAATCAATACTCACATTCATGACTTCATCAACAAATTCTGACCTCCATTTGCATTGTTGTATGGGTGAATTCACCTGATGATAATGTTTATCATCTTCTTTTCATTGGATCTTCTTTCTATTGGATCTTCTTTATGTTGGATGAAGTCCCATGCTTATCATCTTGTTTATCATCTTCTTTCTATTGGATCTTCTTTTTGTTGGATGAAGTCCCA

mRNA sequence

GCACGTTATTCACGAGGCCGTTATTTCTCCGGCGAAAAGGATCTTAATACATTCAACGCCGATCTATTTATAGTTCTCTTAAAGAAATTGATTGAAAGTGAACCAGAGAGAGCAACTGCCATGGACGCCATTGAACACACCACCGTCAACACCAATGGCATCAAAATGCACATTGCCTCCATAGGCACAGGCCCCGTCGTTCTTCTTCTCCACGGCTTTCCCGAGCTCTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCTTCCGTCGGATATCGAGCCATTGCACCGGACCTCCGCGGCTATGGCGATACCGACTCTCCAGAATCGCATACTTCCTACACTGCGCTCCATATCGTCGGTGATTTGGTTGGGGCTTTGGACGAGCTTGGTATTGAGAAGGTGTTTCTTGTAGGGCACGATTGGGGGGCGATCATCGCGTGGTACTTTTGCCTGTTTAGACCTGATAGAATCAAGGCCTTGGTGAATCTGAGTGTTCAGTTCTTCCCTAGAAATCCGAAAATTTCTTTCATCGAACGATTCAGAGCTGTGTATGGTGATGATTTCTATATGTGCAGGTTTCAGGTTCCAGGGGAGGCAGAAGCAGATTTTTTCTGCATCGACACAGCTCAACTCTTCAAGTCAACACTATCTAATAGAAGTACTCATGCTCCATGCTTGCCTAAAGAGTACGGATTTCGCGCGATCCCGCCGCCAGAAAACCTTCCATCTTGGTTGACGGAAGAAGATATCAACCATTATGCTTCCAAATTTAAGGAGACGGGCTTCACTGGAGCATTGAACTACTATCGCGCTTTTGACCTAACCTGGGAGCTGACGGGGCCATGGACGGGAGCACAGATTCAGGTACCTGTGAAATTCATCGTTGGAGATTTGGATATAACGTATCATTTTGAAGGAGCCAAGGAATATATCCATAATGGGGGATTCAAAAGGGACGTGCCATTGCTGGAGGAAGTTGTTATTGTAGAAGATGCTGGTCACTTTGTACACGAAGAAAGGCCTCATGAAATCAATACTCACATTCATGACTTCATCAACAAATTCTGACCTCCATTTGCATTGTTGTATGGGTGAATTCACCTGATGATAATGTTTATCATCTTCTTTTCATTGGATCTTCTTTCTATTGGATCTTCTTTATGTTGGATGAAGTCCCATGCTTATCATCTTGTTTATCATCTTCTTTCTATTGGATCTTCTTTTTGTTGGATGAAGTCCCA

Coding sequence (CDS)

ATGGACGCCATTGAACACACCACCGTCAACACCAATGGCATCAAAATGCACATTGCCTCCATAGGCACAGGCCCCGTCGTTCTTCTTCTCCACGGCTTTCCCGAGCTCTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCTTCCGTCGGATATCGAGCCATTGCACCGGACCTCCGCGGCTATGGCGATACCGACTCTCCAGAATCGCATACTTCCTACACTGCGCTCCATATCGTCGGTGATTTGGTTGGGGCTTTGGACGAGCTTGGTATTGAGAAGGTGTTTCTTGTAGGGCACGATTGGGGGGCGATCATCGCGTGGTACTTTTGCCTGTTTAGACCTGATAGAATCAAGGCCTTGGTGAATCTGAGTGTTCAGTTCTTCCCTAGAAATCCGAAAATTTCTTTCATCGAACGATTCAGAGCTGTGTATGGTGATGATTTCTATATGTGCAGGTTTCAGGTTCCAGGGGAGGCAGAAGCAGATTTTTTCTGCATCGACACAGCTCAACTCTTCAAGTCAACACTATCTAATAGAAGTACTCATGCTCCATGCTTGCCTAAAGAGTACGGATTTCGCGCGATCCCGCCGCCAGAAAACCTTCCATCTTGGTTGACGGAAGAAGATATCAACCATTATGCTTCCAAATTTAAGGAGACGGGCTTCACTGGAGCATTGAACTACTATCGCGCTTTTGACCTAACCTGGGAGCTGACGGGGCCATGGACGGGAGCACAGATTCAGGTACCTGTGAAATTCATCGTTGGAGATTTGGATATAACGTATCATTTTGAAGGAGCCAAGGAATATATCCATAATGGGGGATTCAAAAGGGACGTGCCATTGCTGGAGGAAGTTGTTATTGTAGAAGATGCTGGTCACTTTGTACACGAAGAAAGGCCTCATGAAATCAATACTCACATTCATGACTTCATCAACAAATTCTGA

Protein sequence

MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNRSTHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELTGPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEERPHEINTHIHDFINKF
Homology
BLAST of CmaCh14G009130 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 119/326 (36.50%), Postives = 179/326 (54.91%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGT--GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 60
           M A     V+TNG+++ +   G    PVV+L HGFPEL YSWRHQ+  L+  GY  +APD
Sbjct: 1   MGAPTERLVDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPD 60

Query: 61  LRGYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRI 120
            RGYG +  PE+  +Y    +  DLVG LD++G E+   VGHDWGA++ W   L   DR+
Sbjct: 61  QRGYGGSSRPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRV 120

Query: 121 KALVNLSVQFFPRNPKISFIERFRAVYGDD-FYMCRFQVPGEAEADFFCIDTAQLFKSTL 180
            A+  LSV   PR  ++   + FR+ +G++ FY+  FQ PG A+A+    D A+  +  +
Sbjct: 121 AAVAALSVPALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNG-DPARTMRRMI 180

Query: 181 -------SNRSTHAPCLPKEYGF-RAIPPPENLPSWLTEEDINHYASKFKETGFTGALNY 240
                     +      P   GF   +P P  LP+W+++E+++HY  +F  TGFTG LN+
Sbjct: 181 GGLRPPGDQSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNW 240

Query: 241 YRAFDLTWELTGPWTGAQIQVPVKFIVGDLD--ITYHFEGAKEYIHNGGFKRDVPLLEEV 300
           YR FD  WE T    G  I VP  FI G  D  +T+        + +G ++         
Sbjct: 241 YRNFDRNWETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISGPYRE-------- 300

Query: 301 VIVEDAGHFVHEERPHEINTHIHDFI 314
           V+++ AGH++ +ERP E+   + +F+
Sbjct: 301 VLIDGAGHWLQQERPGEVTAALLEFL 316

BLAST of CmaCh14G009130 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 194.5 bits (493), Expect = 1.8e-48
Identity = 103/306 (33.66%), Postives = 162/306 (52.94%), Query Frame = 0

Query: 14  IKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHTS 73
           +++H   +G+GP V L HGFPE WYSWR+Q+  L+  GYR +A D++GYG++ +P     
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRNP 133
           Y    +  ++V  LD+LG+ +   +GHDWG ++ WY  LF P+R++A+ +L+  F P NP
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 KISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTL-----SNRSTHAPCLP 193
            +S +E  +A    D Y   FQ PG AEA+    + ++ FKS       S  S H  C  
Sbjct: 368 NMSPLESIKANPVFD-YQLYFQEPGVAEAELE-QNLSRTFKSLFRASDESVLSMHKVC-- 427

Query: 194 KEYGFRAIPPPE--NLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELTGPWTGA 253
            E G   +  PE  +L   +TEE+I  Y  +FK++GF G LN+YR  +  W+      G 
Sbjct: 428 -EAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGR 487

Query: 254 QIQVPVKFIVGDLD------ITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 307
           +I +P   +  + D      ++ H E    ++  G              +ED GH+   +
Sbjct: 488 KILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGH-------------IEDCGHWTQMD 535

BLAST of CmaCh14G009130 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-47
Identity = 105/299 (35.12%), Postives = 166/299 (55.52%), Query Frame = 0

Query: 13  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHT 72
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++GYGD+ SP    
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRN 132
            Y    +  ++V  LD+LGI +   +GHDW  ++ W   LF P+R++A+ +L+  F P +
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTL-SNRSTHAPCLPK-- 192
           P +S ++  R++   + Y   FQ PG AEA+    + ++ FKS   ++  T    + K  
Sbjct: 365 PDVSPMKVIRSIPVFN-YQLYFQEPGVAEAELE-KNMSRTFKSFFRASDETGFIAVHKAT 424

Query: 193 EYGFRAIPPPE--NLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELTGPWTGAQ 252
           E G   +  PE  NL    TEE+I  Y  +FK+TGF G LN+YR  +  W+ +    G +
Sbjct: 425 EIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRK 484

Query: 253 IQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEERPHEIN 307
           I VP   +  + DI    E +K        ++ +P L+   I ED GH+   E+P E+N
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKRGHI-EDCGHWTQIEKPTEVN 534

BLAST of CmaCh14G009130 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.6e-47
Identity = 101/302 (33.44%), Postives = 163/302 (53.97%), Query Frame = 0

Query: 13  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHT 72
           G+++H   +G+GP V L HGFPE W+SWR+Q+  L+  G+R +A D++GYG++ +P    
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRN 132
            Y+   +  D+V  L++LG+ +   +GHDWG ++ W   LF P+R++A+ +L+  F P N
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSN--RSTHAPCLP-- 192
           P +S +E  +A    D Y   FQ PG AEA+       Q    T  N  R+     L   
Sbjct: 367 PNVSPMEIIKANPVFD-YQLYFQEPGVAEAEL-----EQNLDRTFKNFFRAHDETFLTTN 426

Query: 193 --KEYG--FRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELTGPWT 252
             +E G  F   P   +L   +TEEDI  Y  +FK++GF G LN+YR  +  W+     +
Sbjct: 427 RVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGS 486

Query: 253 GAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEERPHE 307
           G +I +P   +  + D+  H + +K        +  +P L+   I +D GH+   ++P E
Sbjct: 487 GRKILIPALMVTAENDLVLHPKMSKH------MENWIPHLKRGHI-KDCGHWTQIDKPAE 535

BLAST of CmaCh14G009130 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 5.8e-47
Identity = 101/299 (33.78%), Postives = 163/299 (54.52%), Query Frame = 0

Query: 13  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPESHT 72
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++GYGD+ SP    
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKALVNLSVQFFPRN 132
            Y    +  ++V  L++LGI +   +GHDW  ++ W   LF P+R++A+ +L+    P N
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKS---TLSNRSTHAPCLPK 192
           P++S +E  R++   + Y   FQ PG AEA+    + ++ FKS   T  +          
Sbjct: 365 PEVSPMEVIRSIPVFN-YQLYFQEPGVAEAELE-KNMSRTFKSFFRTSDDMGLLTVNKAT 424

Query: 193 EYGFRAIPPPEN--LPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELTGPWTGAQ 252
           E G   +  PE+  +    TEE+I +Y  +FK++GF G LN+YR  +  W+ +    G +
Sbjct: 425 EMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRK 484

Query: 253 IQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEERPHEIN 307
           I VP   +  + DI    E +K        +  +P L+   I ED GH+   E+P E+N
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMENWIPFLKRGHI-EDCGHWTQIEKPAEVN 534

BLAST of CmaCh14G009130 vs. ExPASy TrEMBL
Match: A0A6J1J139 (uncharacterized protein LOC111482485 OS=Cucurbita maxima OX=3661 GN=LOC111482485 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 2.9e-190
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR
Sbjct: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
           GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           RPHEINTHIHDFINKF
Sbjct: 301 RPHEINTHIHDFINKF 316

BLAST of CmaCh14G009130 vs. ExPASy TrEMBL
Match: A0A6J1F1J8 (uncharacterized protein LOC111441539 OS=Cucurbita moschata OX=3662 GN=LOC111441539 PE=4 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 6.0e-188
Identity = 311/316 (98.42%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEHTT+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNP+ISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFK TLSNR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           STHAPCLPKEYGFRAIPPPE+LPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPESLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
           GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           RPHEINTHIHDF NKF
Sbjct: 301 RPHEINTHIHDFFNKF 316

BLAST of CmaCh14G009130 vs. ExPASy TrEMBL
Match: A0A5D3E0A4 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001200 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 3.3e-178
Identity = 293/316 (92.72%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+HTT+ TNGIKMHIAS+G GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRA YGDDFYMCRFQVPGEAEADF CIDTAQLFK+TLSNR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           ST APCLPKEYGFRAIPPPENLPSWLTEEDIN+YA+KFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
            PWTG QIQVPVKFIVGD D+TYHF+GAKEYIH GGFKRDVPLLEEVVIVE+AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           +PHEINTHIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of CmaCh14G009130 vs. ExPASy TrEMBL
Match: A0A1S3BZJ8 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494757 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 3.3e-178
Identity = 293/316 (92.72%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+HTT+ TNGIKMHIAS+G GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRA YGDDFYMCRFQVPGEAEADF CIDTAQLFK+TLSNR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           ST APCLPKEYGFRAIPPPENLPSWLTEEDIN+YA+KFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
            PWTG QIQVPVKFIVGD D+TYHF+GAKEYIH GGFKRDVPLLEEVVIVE+AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           +PHEINTHIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of CmaCh14G009130 vs. ExPASy TrEMBL
Match: A0A0A0KT70 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001920 PE=4 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 4.3e-178
Identity = 293/316 (92.72%), Postives = 306/316 (96.84%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+HTT+NTNGIKMHIASIG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP SHTSYTALHIVGDL+GALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRA YGDDFYMCRFQVPGEAEADF CIDTAQLFK+TLS R
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSTR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           ST APCLPKEYGFRAIPPPENLPSWLTEEDIN+YA+KFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
            PWTG QIQVPVKFIVGDLDITYHF+GAKEYIH+GGFKRDVPLLEEVVIV++AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           +PHEINTHIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of CmaCh14G009130 vs. NCBI nr
Match: XP_022984062.1 (uncharacterized protein LOC111482485 [Cucurbita maxima])

HSP 1 Score: 674.1 bits (1738), Expect = 5.9e-190
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR
Sbjct: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
           GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           RPHEINTHIHDFINKF
Sbjct: 301 RPHEINTHIHDFINKF 316

BLAST of CmaCh14G009130 vs. NCBI nr
Match: XP_022934346.1 (uncharacterized protein LOC111441539 [Cucurbita moschata] >KAG6581279.1 hypothetical protein SDJN03_21281, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 666.4 bits (1718), Expect = 1.2e-187
Identity = 311/316 (98.42%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEHTT+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNP+ISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFK TLSNR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           STHAPCLPKEYGFRAIPPPE+LPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPESLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
           GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           RPHEINTHIHDF NKF
Sbjct: 301 RPHEINTHIHDFFNKF 316

BLAST of CmaCh14G009130 vs. NCBI nr
Match: XP_023528764.1 (uncharacterized protein LOC111791602 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 666.0 bits (1717), Expect = 1.6e-187
Identity = 311/316 (98.42%), Postives = 313/316 (99.05%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIEHTT+NTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNP+ISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFK TLSNR
Sbjct: 121 LVNLSVQFFPRNPEISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKVTLSNR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
           GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIH GGFKRDVPLLEEVVIVEDAGHFVHEE
Sbjct: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHKGGFKRDVPLLEEVVIVEDAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           RPHEINTHIHDF NKF
Sbjct: 301 RPHEINTHIHDFFNKF 316

BLAST of CmaCh14G009130 vs. NCBI nr
Match: XP_008454322.1 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044389.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK29517.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 634.0 bits (1634), Expect = 6.8e-178
Identity = 293/316 (92.72%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+HTT+ TNGIKMHIAS+G GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRA YGDDFYMCRFQVPGEAEADF CIDTAQLFK+TLSNR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           ST APCLPKEYGFRAIPPPENLPSWLTEEDIN+YA+KFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
            PWTG QIQVPVKFIVGD D+TYHF+GAKEYIH GGFKRDVPLLEEVVIVE+AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           +PHEINTHIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of CmaCh14G009130 vs. NCBI nr
Match: XP_004152242.1 (uncharacterized protein LOC101204329 [Cucumis sativus] >KGN52825.1 hypothetical protein Csa_014782 [Cucumis sativus])

HSP 1 Score: 633.6 bits (1633), Expect = 8.8e-178
Identity = 293/316 (92.72%), Postives = 306/316 (96.84%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAI+HTT+NTNGIKMHIASIG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGDTDSP SHTSYTALHIVGDL+GALDELGIEKVFLVGHDWGAIIAWYFCLFRP+RIKA
Sbjct: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVNLSVQFFPRNP ISFI+RFRA YGDDFYMCRFQVPGEAEADF CIDTAQLFK+TLS R
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSTR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           ST APCLPKEYGFRAIPPPENLPSWLTEEDIN+YA+KFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
            PWTG QIQVPVKFIVGDLDITYHF+GAKEYIH+GGFKRDVPLLEEVVIV++AGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300

Query: 301 RPHEINTHIHDFINKF 317
           +PHEINTHIHDFI KF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of CmaCh14G009130 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 464.5 bits (1194), Expect = 6.6e-131
Identity = 198/316 (62.66%), Postives = 251/316 (79.43%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ IEHTT++TNGI MH+ASIG+GPV+L +HGFP+LWYSWRHQL+  +++GYRAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120
           GYGD+D+P S  SYT LHIVGDLVG LD LG+++VFLVGHDWGAI+AW+ C+ RPDR+ A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKSTLSNR 180
           LVN SV F PRNP +  ++ FRA++GDD+Y+CRFQ PGE E DF  +DT +L     ++R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 STHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAFDLTWELT 240
           +   PC+PK  GFR +P P +LP+WLTE+D+  Y  KF + GFTG LNYYRA +L+WELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 241 GPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDAGHFVHEE 300
            PWTG QI+VPVKFIVGDLDITY+  G KEYIH GG K+ VP L+EVV++E  GHF+H+E
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 301 RPHEINTHIHDFINKF 317
           +P E+  HI+ F  KF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

BLAST of CmaCh14G009130 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 401.7 bits (1031), Expect = 5.3e-112
Identity = 183/323 (56.66%), Postives = 234/323 (72.45%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 60
           M+ I+H  V+ NGI MHIA  G   GPVVLLLHGFP+LWY+WRHQ+  LSS+GYRA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDTDSPESHTSYTALHIVGDLVGALDEL--GIEKVFLVGHDWGAIIAWYFCLFRPD 120
           LRGYGD+DSPES + YT L++VGDLV  LD +    EKVFLVGHDWGAII W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RIKALVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKST 180
           +I   V LSV +  RNPK+  ++ F+AV+GDD+Y+CRFQ PG+ E +    D     ++ 
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LSNRSTHAPCLPKEYGFRAIPPPEN----LPSWLTEEDINHYASKFKETGFTGALNYYRA 240
            + R+   P LPK+  F   P P +    LP W +++D++ Y SKF++ GFTG LNYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 FDLTWELTGPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVED 300
            DL WELT PWTGA+IQVPVKF+ GD D+ Y   G KEYIH GGF  DVP L+E+V++ED
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 AGHFVHEERPHEINTHIHDFINK 316
           AGHFV++E+P E+  HI+DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of CmaCh14G009130 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.8 bits (974), Expect = 2.1e-105
Identity = 174/324 (53.70%), Postives = 232/324 (71.60%), Query Frame = 0

Query: 1   MDAIEHTTVNTNGIKMHIA-----SIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAI 60
           +D +EH T+  NGI MH+A       G  P++L LHGFPELWY+WRHQ++ LSS+GYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDTDSPESHTSYTALHIVGDLVGALDEL--GIEKVFLVGHDWGAIIAWYFCLF 120
           APDLRGYGDT++PE    YT L++ GD+V  +D +  G + V +VGHDWGA+IAW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRIKALVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLF 180
           RP+++KALVN+SV F PRNP    +   R V+GDD+Y+CRFQ  GE E +F  + T  + 
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KSTLSNRSTHAPCLPKE-YGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRA 240
           K  L+ ++     LPK+ Y  R+      LP WLT+ED+++Y +K++  GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 FDLTWELTGPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVED 300
            D  WELT PWTGA+I+VPVKFI+GD D+TY+F GAKEYI+ GGFKRDVPLL+E V+++ 
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AGHFVHEERPHEINTHIHDFINKF 317
            GHF+HEE P  IN HIH+F +KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of CmaCh14G009130 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 366.7 bits (940), Expect = 1.9e-101
Identity = 176/325 (54.15%), Postives = 229/325 (70.46%), Query Frame = 0

Query: 4   IEHTTVNTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRG 63
           +EH  V  NGI +H+A  G   GP+VLLLHGFPELWYSWRHQ+  L++ GYRA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDTDSPESHTSYTALHIVGDLVGALDELGI---EKVFLVGHDWGAIIAWYFCLFRPDRI 123
           YGD+D+P   +SYT  +IVGDL+  +  L     EKVF+VGHDWGA+IAWY CLFRPDR+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSV--QFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLFKST 183
           KALVNLSV   F P +P +  ++R RA YGDD+Y+CRFQ  G+ EA+   + T ++ K  
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LSNRSTHAPCLPKEYGF-----RAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYR 243
           L+ R+     +PK+  F       IP    LPSWLTEED+ ++ SKF+E GF+G +NYYR
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIP----LPSWLTEEDVAYFVSKFEEKGFSGPVNYYR 240

Query: 244 AFDLTWELTGPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVE 303
            F+   EL GPW G++IQVP KF++G+LD+ Y+  G KEYIH   FK DVPLLEE V++E
Sbjct: 241 NFNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVME 300

Query: 304 DAGHFVHEERPHEINTHIHDFINKF 317
              HF+++E+P EI   I DFI+KF
Sbjct: 301 GVAHFINQEKPQEILQIILDFISKF 321

BLAST of CmaCh14G009130 vs. TAIR 10
Match: AT4G15955.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.3e-99
Identity = 174/323 (53.87%), Postives = 222/323 (68.73%), Query Frame = 0

Query: 5   EHTTVNTNGIKMHIAS-----IGTG----PVVLLLHGFPELWYSWRHQLLYLSSVGYRAI 64
           +H+ V  NGI MH+A       G G    PV+L LHGFPELWY+WRHQ++ LSS+GYR I
Sbjct: 6   DHSFVKVNGITMHVAEKSPSVAGNGAIRPPVILFLHGFPELWYTWRHQMVALSSLGYRTI 65

Query: 65  APDLRGYGDTDSPESHTSYTALHIVGDLVGALDEL--GIEKVFLVGHDWGAIIAWYFCLF 124
           APDLRGYGDTD+PES  +YT+LH+VGDL+G +D +    EKVF+VGHDWGAIIAW+ CLF
Sbjct: 66  APDLRGYGDTDAPESVDAYTSLHVVGDLIGLIDAVVGDREKVFVVGHDWGAIIAWHLCLF 125

Query: 125 RPDRIKALVNLSVQFFPRNPKISFIERFRAVYGDDFYMCRFQVPGEAEADFFCIDTAQLF 184
           RPDR+KALVN+SV F P NPK      F+A YGDD+Y+CRFQ+               L 
Sbjct: 126 RPDRVKALVNMSVVFDPWNPKRKPTSTFKAFYGDDYYICRFQL------------LEILI 185

Query: 185 KSTLSNRSTHAPCLPKEYGFRAIPPPENLPSWLTEEDINHYASKFKETGFTGALNYYRAF 244
           K        H   + K Y         +LPSWLT+ D+ +Y SK+++ GFTG +NYYR  
Sbjct: 186 K-------IHVCIVGKRY-----DDSVSLPSWLTDSDVKYYVSKYEKNGFTGPVNYYRNM 245

Query: 245 DLTWELTGPWTGAQIQVPVKFIVGDLDITYHFEGAKEYIHNGGFKRDVPLLEEVVIVEDA 304
           D TWEL G  + A+++VPVKFI+GD D+TYH  G+K+YIH+G FK  VPLL+EVV+++  
Sbjct: 246 DRTWELMGSLSNAKVKVPVKFIIGDQDLTYHIPGSKKYIHDGRFKSHVPLLDEVVVIKGV 304

Query: 305 GHFVHEERPHEINTHIHDFINKF 317
           GHF+HEERP EI+ HIHD+   F
Sbjct: 306 GHFIHEERPDEISKHIHDYFLTF 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I6YGS01.4e-5636.50Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
P349131.8e-4833.66Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349141.2e-4735.12Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
Q6Q2C22.6e-4733.44Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P802995.8e-4733.78Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1J1392.9e-190100.00uncharacterized protein LOC111482485 OS=Cucurbita maxima OX=3661 GN=LOC111482485... [more]
A0A6J1F1J86.0e-18898.42uncharacterized protein LOC111441539 OS=Cucurbita moschata OX=3662 GN=LOC1114415... [more]
A0A5D3E0A43.3e-17892.72Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BZJ83.3e-17892.72bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494757 PE... [more]
A0A0A0KT704.3e-17892.72AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001... [more]
Match NameE-valueIdentityDescription
XP_022984062.15.9e-190100.00uncharacterized protein LOC111482485 [Cucurbita maxima][more]
XP_022934346.11.2e-18798.42uncharacterized protein LOC111441539 [Cucurbita moschata] >KAG6581279.1 hypothet... [more]
XP_023528764.11.6e-18798.42uncharacterized protein LOC111791602 [Cucurbita pepo subsp. pepo][more]
XP_008454322.16.8e-17892.72PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044389.1 bi... [more]
XP_004152242.18.8e-17892.72uncharacterized protein LOC101204329 [Cucumis sativus] >KGN52825.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
AT4G02340.16.6e-13162.66alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.15.3e-11256.66alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.12.1e-10553.70alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.11.9e-10154.15soluble epoxide hydrolase [more]
AT4G15955.32.3e-9953.87alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 111..124
score: 26.85
coord: 97..110
score: 33.0
coord: 51..66
score: 44.61
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..300
e-value: 3.8E-22
score: 79.2
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 51..66
score: 60.8
coord: 97..110
score: 52.92
coord: 31..49
score: 55.26
coord: 111..124
score: 49.35
coord: 290..312
score: 39.53
coord: 250..266
score: 38.24
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..316
e-value: 3.0E-107
score: 361.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 3..315
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..316
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G009130.1CmaCh14G009130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity