CmaCh14G009040 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh14G009040 vs. ExPASy Swiss-Prot
Match: Q9SC88 (Gamma-tubulin complex component 4 homolog OS=Medicago truncatula OX=3880 GN=85P PE=2 SV=1)

HSP 1 Score: 1052.7 bits (2721), Expect = 1.9e-306
Identity = 548/758 (72.30%), Postives = 627/758 (82.72%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIID R+       N+L  + PIS E TFKLAPDISF++ S+R+L
Sbjct: 1   MLHELLLALLGYTGDLIIDRRD-------NNLSANTPISDECTFKLAPDISFIDPSDREL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           I+RI+ LGFYYREL+RF+ KSRNL+WIRS + + L N         E PSVYRRA+ANGI
Sbjct: 61  IERIITLGFYYRELERFSAKSRNLNWIRSENANPLENK--------EKPSVYRRALANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEIL+VY S++LH+EQ LLSET+PILA VTQGL+KFF L PPL++L+L+IER DIRGG+L
Sbjct: 121 VEILAVYSSSILHIEQLLLSETMPILATVTQGLNKFFSLLPPLYELILKIERGDIRGGEL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHK+CHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGIL+D+HGEFFI RQ+ R+ 
Sbjct: 181 LNLLHKKCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILEDRHGEFFISRQEGRDV 240

Query: 241 DQGSAVQVLSEKLGRLST-DESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLR 300
           +  S+ Q +SEKL RLST D SL+DWH+GFHI LDMLP+YI M+VAESILFAGKA+RVLR
Sbjct: 241 ENSSSHQEISEKLSRLSTADASLSDWHMGFHISLDMLPEYIPMRVAESILFAGKAVRVLR 300

Query: 301 NPSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIE 360
           NPS +F  Q   +  +   P+   KI G    F  Q+EP   T +  E+LL QSEADKIE
Sbjct: 301 NPSPSFLSQ---DDVYPQEPKRFPKIHGFEGRFNFQREPIINTGMRVEDLLPQSEADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
            MLLDLKESSEFHKRSFE A+DSI+AIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF
Sbjct: 361 NMLLDLKESSEFHKRSFECAVDSIQAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420

Query: 421 FQCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVK- 480
           FQCFLEES  LMRLPPRQSTAEADLM+PFQLA+ KT  +ED+YFS+VSLRMP +G+ VK 
Sbjct: 421 FQCFLEESRQLMRLPPRQSTAEADLMVPFQLASLKTIGEEDKYFSKVSLRMPSYGITVKP 480

Query: 481 SAQGDPK-EKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCR 540
           S    PK      DG  G ++SN SS+MS+DGW+G+ALEY I+WPL LFFTQEVLS+Y +
Sbjct: 481 SLLNVPKATSAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSRYLK 540

Query: 541 VFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQR-QHFRQMWRVREH 600
           VFQYLLRLKRTQMELEK WAS+MHQ H+ F+KN+ +  D S   QQR Q FR MWRVREH
Sbjct: 541 VFQYLLRLKRTQMELEKLWASVMHQYHSIFAKNKKSDQDKSPITQQRDQRFRSMWRVREH 600

Query: 601 MAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVS 660
           MAFLIRNLQFYIQVDVIESQWNILQ  IQDSHDFTELVGFHQEYLSALISQ+FLDIGSVS
Sbjct: 601 MAFLIRNLQFYIQVDVIESQWNILQSHIQDSHDFTELVGFHQEYLSALISQTFLDIGSVS 660

Query: 661 RILDGIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPF 720
           RILDGIMKLCLQFCW+IENQ+  S+ SELEH+ EEFNKKSNSLYTILRSSRL GSQR PF
Sbjct: 661 RILDGIMKLCLQFCWNIENQDNFSNTSELEHIAEEFNKKSNSLYTILRSSRLAGSQRTPF 720

Query: 721 LRRFLMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           LRRFL+RLN NSFFE+TA+ V+NVVR RP   P LNQ+
Sbjct: 721 LRRFLLRLNLNSFFESTAKEVMNVVRPRP-TFPGLNQR 739

BLAST of CmaCh14G009040 vs. ExPASy Swiss-Prot
Match: Q9M350 (Gamma-tubulin complex component 4 OS=Arabidopsis thaliana OX=3702 GN=GCP4 PE=2 SV=2)

HSP 1 Score: 1048.1 bits (2709), Expect = 4.7e-305
Identity = 550/750 (73.33%), Postives = 622/750 (82.93%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLG+TGDLI+DERE    LGL     D+P+S E TFKLAPDISF+E SERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGL-AFNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRS-SHGSSLPNSVESSKDKTE-IPSVYRRAIAN 120
           I+R++ LGFYYRELDRFA KSRNLSWIRS +    L  + E SK   E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGG 180
           GI EILSVYRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL++++LEIERDDIRGG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQVMYNQLA+WMVYGILQD HGEFFI+RQDD 
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETDQGSAVQVLSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRV 300
           + D  S+ + +SEKL R S  E SLTDWH GFHI LDMLPDYI M++ ESILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADK 360
           LRNPS AF  Q   ++S     R   +I+G   +   + E      LTG ELL QSEADK
Sbjct: 301 LRNPSPAF--QFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGELLPQSEADK 360

Query: 361 IEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKG 420
           IEAML DLKESSEFHKRSFE  +DS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL KG
Sbjct: 361 IEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEKG 420

Query: 421 DFFQCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKV 480
           DFFQCFLEES  LMRLPPRQST E+DLM+PFQLAATKT ++ED+YFSRVSLRMP FGV V
Sbjct: 421 DFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVTV 480

Query: 481 KSAQGD-PKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYC 540
           +S+Q D  + K    G      +NL+SD S+DGW+ +ALEY +DWP+QLFFTQEVLSKY 
Sbjct: 481 RSSQADMVRSKVSLTGK-----ANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSKYL 540

Query: 541 RVFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREH 600
           +VFQYL+RLKRTQMELEKSWAS+MHQDH + +++R    +GS S Q+RQ  R MWRVREH
Sbjct: 541 KVFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVREH 600

Query: 601 MAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVS 660
           MAFLIRNLQFYIQVDVIESQW +LQ  I DS DFTELVGFHQEYLSALISQSFLDIGSVS
Sbjct: 601 MAFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGSVS 660

Query: 661 RILDGIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPF 720
           RILD IMKLCLQFCW+IENQ  + + SELE++ EEFNKKSNSLYTILRSS+L GSQRAPF
Sbjct: 661 RILDSIMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRAPF 720

Query: 721 LRRFLMRLNFNSFFEATARGVLNVVRTRPA 747
           LRRFL+RLNFNSF+EATARGVLNVVR RPA
Sbjct: 721 LRRFLLRLNFNSFYEATARGVLNVVRQRPA 742

BLAST of CmaCh14G009040 vs. ExPASy Swiss-Prot
Match: Q9D4F8 (Gamma-tubulin complex component 4 OS=Mus musculus OX=10090 GN=Tubgcp4 PE=1 SV=2)

HSP 1 Score: 316.2 bits (809), Expect = 9.8e-85
Identity = 226/749 (30.17%), Postives = 370/749 (49.40%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL  SE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           + R+  LG  Y     F  +       +  H           + +  +  +Y RA   G+
Sbjct: 61  LNRLCRLGTDYIRFTEFIEQYTGHVQQQDHH--------PPQQGQGGLHGIYLRAFCTGL 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
             +L  YR A+L +EQ+ L++    ++ V   LD+F +LFP +  +V +I+   I G Q+
Sbjct: 121 DSVLQPYRQALLDLEQEFLADPHLSISHVNYSLDQFQLLFPSVMVVVEQIKSQKIHGCQI 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIR------- 240
           L  ++K    G+P +++ ++++L   H VMY QL++WM++G+L D+H EFFI+       
Sbjct: 181 LETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGT 240

Query: 241 ---RQDDRETDQG----SAVQVLSEKLGRLSTDESLTDWHL-GFHIYLDMLPDYIHMKVA 300
              + ++ E D G    +  Q+   +  RL  +E++    L  F + +++LP YI ++VA
Sbjct: 241 LSAQLEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRVA 300

Query: 301 ESILFAGKAIRVLRNPSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLT 360
           E ILF G+++++  N +                                         LT
Sbjct: 301 EKILFVGESVQMFENQN---------------------------------------VNLT 360

Query: 361 GEELLLQSEADKIEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHL 420
            +  +L+++ D   A L  LK+   F    FE  +D IR+  A HLW+L+V  +DL G L
Sbjct: 361 RKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQL 420

Query: 421 KALKDYFLLAKGDFFQCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSR 480
           K +KD++LL +G+ FQ F++ + H+++ PP   T E D+ + FQ +A K   D+D     
Sbjct: 421 KIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNLLPL 480

Query: 481 VSLRMPLFGVKVKSAQGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQL 540
           + L +   G   K+    P+E P  + +P  A S+        GW  + L Y + WPL +
Sbjct: 481 LHLTIEYHGKDHKADATQPREVPSRETSPREAPSS--------GWAALGLSYKVQWPLHI 540

Query: 541 FFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQ 600
            FT  VL KY  VF+YLL ++R Q EL+  WA  M + H                 +  Q
Sbjct: 541 LFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHL----------------KSNQ 600

Query: 601 HFRQMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALI 660
                WR+R HMAFL+ NLQ+Y+QVDV+ESQ++ L  +I  + DF  +   H  +LS L+
Sbjct: 601 TDAVKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNLL 655

Query: 661 SQSFLDIGSVSRILDGIMKLCLQFCWSIENQN----KSSDPSELEHLTEEFNKKSNSLYT 720
           +QSF+ +  V   L+ I+ LC  FC S+ +QN         ++L  L + F+++S+ L+ 
Sbjct: 661 AQSFILLKPVFHCLNEILDLCHSFC-SLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFK 655

Query: 721 ILRSSRLVGSQRAPFLRRFLMRLNFNSFF 731
           IL S R    Q    L + L+RL++N ++
Sbjct: 721 ILSSVR--NHQINSDLAQLLLRLDYNKYY 655

BLAST of CmaCh14G009040 vs. ExPASy Swiss-Prot
Match: Q9UGJ1 (Gamma-tubulin complex component 4 OS=Homo sapiens OX=9606 GN=TUBGCP4 PE=1 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 8.3e-84
Identity = 225/749 (30.04%), Postives = 370/749 (49.40%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL  SE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           + R+  LG  Y     F  +       +  H         S + +  +  +Y RA   G+
Sbjct: 61  LNRLCRLGTDYIRFTEFIEQYTGHVQQQDHH--------PSQQGQGGLHGIYLRAFCTGL 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
             +L  YR A+L +EQ+ L +    ++ V   LD+F +LFP +  +V +I+   I G Q+
Sbjct: 121 DSVLQPYRQALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQI 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIR------- 240
           L  ++K    G+P +++ ++++L   H VMY QL++WM++G+L D+H EFFI+       
Sbjct: 181 LETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGN 240

Query: 241 ---RQDDRETDQG----SAVQVLSEKLGRLSTDESLTDWHL-GFHIYLDMLPDYIHMKVA 300
              + ++ E D G    +  Q+   +  RL  +E++    L  F + +++LP YI ++VA
Sbjct: 241 VSAQPEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVRVA 300

Query: 301 ESILFAGKAIRVLRNPSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLT 360
           E ILF G+++++  N +                                         LT
Sbjct: 301 EKILFVGESVQMFENQN---------------------------------------VNLT 360

Query: 361 GEELLLQSEADKIEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHL 420
            +  +L+++ D   A L  LK+   F    FE  +D IR+  A HLW+L+V  +DL G L
Sbjct: 361 RKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQL 420

Query: 421 KALKDYFLLAKGDFFQCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSR 480
           K +KD++LL +G+ FQ F++ + H+++ PP   T E D+ + FQ +A K   D+D     
Sbjct: 421 KIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNLLPL 480

Query: 481 VSLRMPLFGVKVKSAQGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQL 540
           + L +   G + K+     +E P  + +P  A ++        GW  + L Y + WPL +
Sbjct: 481 LHLTIEYHGKEHKADATQAREGPSRETSPREAPAS--------GWAALGLSYKVQWPLHI 540

Query: 541 FFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQ 600
            FT  VL KY  VF+YLL ++R Q EL+  WA  M + H                 +  Q
Sbjct: 541 LFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHL----------------KSNQ 600

Query: 601 HFRQMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALI 660
                WR+R HMAFL+ NLQ+Y+QVDV+ESQ++ L  +I  + DF  +   H  +LS L+
Sbjct: 601 TDAIKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNLL 655

Query: 661 SQSFLDIGSVSRILDGIMKLCLQFCWSIENQN----KSSDPSELEHLTEEFNKKSNSLYT 720
           +QSF+ +  V   L+ I+ LC  FC S+ +QN         ++L  L + F+++S+ L+ 
Sbjct: 661 AQSFILLKPVFHCLNEILDLCHSFC-SLVSQNLGPLDERGAAQLSILVKGFSRQSSLLFK 655

Query: 721 ILRSSRLVGSQRAPFLRRFLMRLNFNSFF 731
           IL S R    Q    L + L+RL++N ++
Sbjct: 721 ILSSVR--NHQINSDLAQLLLRLDYNKYY 655

BLAST of CmaCh14G009040 vs. ExPASy Swiss-Prot
Match: Q9VKU7 (Gamma-tubulin complex component 4 homolog OS=Drosophila melanogaster OX=7227 GN=Grip75 PE=1 SV=2)

HSP 1 Score: 177.9 bits (450), Expect = 4.2e-43
Identity = 170/702 (24.22%), Postives = 313/702 (44.59%), Query Frame = 0

Query: 52  FLELSERDLIQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSV 111
           F+   ER++   I+ +   Y+E+++F   S   S    +HG  LP+S+            
Sbjct: 31  FIHPCEREIFMDIIKIIKVYQEVEQFTHSSGRKS---DTHG-ELPDSLH---------GY 90

Query: 112 YRRAIANGIVEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIE 171
           Y   +A GI   L  Y + +  +E+  L      L+ V   L   F L   +  L+ EI 
Sbjct: 91  YLLNLAKGIEMALEEYYAEIGRLEKYCLGNERNSLSYVYNALYAKFPLLVFMRNLITEIH 150

Query: 172 RDDIRGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFF 231
             ++RG  LL+ LH++C  G  +L+  I+ ++       ++ LA W+++G++ D H EFF
Sbjct: 151 VLNLRGCVLLHNLHQQCEHGDIQLEKAIKIIMKPVKNAFFSSLAHWLLFGVIDDVHSEFF 210

Query: 232 IRRQDDRETDQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFA 291
           I+       D  S  +  S     LS +++  D+   + + +  LP +  + +AE +LF 
Sbjct: 211 IKFTPTDAVDGSSFSK--SATCSLLSAEKNPEDYIWQYEVNMSQLPGFFSIVLAEKVLFV 270

Query: 292 GKAIRVLRNPSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEEL-- 351
           G+ + V +   N                   +K+K  T   PL  +     +L  +++  
Sbjct: 271 GQTVLVFKMGRN-------------------VKVKNKTD--PLAAK---LAELDSDDIYQ 330

Query: 352 LLQSEADKIEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALK 411
           L      +   M++DL      +    E  I  I+   ++ L ++ V   DL   +  +K
Sbjct: 331 LWSGRESEFFKMVVDLSNEDTINVFRLEKVIIDIKNYVSARLSEIAVNEVDLERQMGLIK 390

Query: 412 DYFLLAKGDFFQCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLR 471
           D+FLL +G+F+  F  +    M     +     ++   F+LAAT T   +D         
Sbjct: 391 DFFLLGRGEFYLEFCSQMVGTME--TYREERFKNVTRSFELAATVTGITDD--------- 450

Query: 472 MPLFGVKVKSAQGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQ 531
           +  F +  + +  +P +               +SD +     G++L+Y  +WPL L F+ 
Sbjct: 451 LDKFSLICQRSTSEPDD---------------TSDFNF--LQGLSLKYEYEWPLNLLFSP 510

Query: 532 EVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQ 591
             + +Y  +F++LL ++  Q E+++ WA           +   A+    + P  +     
Sbjct: 511 TTIERYNNIFRFLLIIRTYQYEIQRVWA----------KQTWRAKSAKDVPPNNK----- 570

Query: 592 MWRVREHMAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSF 651
           +  +R ++ F + N+Q+YIQVDV+ESQ+ IL + I+   DF  +   H  +L+ ++S  F
Sbjct: 571 IITLRNYLMFFLNNMQYYIQVDVLESQFGILMNVIKSRSDFEVIQRAHTVFLANVLSHCF 630

Query: 652 L---------DIGSVSR--ILDGIMKL---CLQFCWSIENQNKSSD-PSELEHLTEEFNK 711
           L           GS +R  I   ++KL   C +F    + ++ S D   E++ L E F  
Sbjct: 631 LLNESETQLNVTGSQNRNPIYGTLLKLFGICEKFAHMTQTKDPSDDLEDEVDQLNESFGV 645

Query: 712 KSNSLYTIL---RSSRLVGSQRAPFLRRFLMRLNFNSFFEAT 734
           +  SL  +L   +S+  +G      L + L+RL+FN +F A+
Sbjct: 691 QIASLIQLLVDVKSASCLGP-----LSQLLLRLDFNCWFSAS 645

BLAST of CmaCh14G009040 vs. ExPASy TrEMBL
Match: A0A6J1J9A7 (Gamma-tubulin complex component OS=Cucurbita maxima OX=3661 GN=LOC111482469 PE=3 SV=1)

HSP 1 Score: 1504.2 bits (3893), Expect = 0.0e+00
Identity = 754/754 (100.00%), Postives = 754/754 (100.00%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI
Sbjct: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA
Sbjct: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480

Query: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 754

BLAST of CmaCh14G009040 vs. ExPASy TrEMBL
Match: A0A6J1F2V2 (Gamma-tubulin complex component OS=Cucurbita moschata OX=3662 GN=LOC111441869 PE=3 SV=1)

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 738/754 (97.88%), Postives = 744/754 (98.67%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIIDEREH+NLLGLNHLPPDAPISGEPTFKLAPDISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRSS+GSSLPNSVESSKDKTEIPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHSHMPRLPLKIKG TRNFPLQKEPFFATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMP FGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGDPKEK YTDGNPGGALSNLSSDMSLDGW+ VALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKTYTDGNPGGALSNLSSDMSLDGWDSVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQ+RQ FRQMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQKRQRFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQ+KSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVV  RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of CmaCh14G009040 vs. ExPASy TrEMBL
Match: A0A5A7SW23 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold228G00060 PE=3 SV=1)

HSP 1 Score: 1391.3 bits (3600), Expect = 0.0e+00
Identity = 701/755 (92.85%), Postives = 725/755 (96.03%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIIDEREH+N LGLN+L  DAPISGEPTFKLA DISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRS + SSL NS ESSKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIERDDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHS+MPRLPL +KG TRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQSAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMP FGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDPKEKPYTDGNPGGAL-SNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGD KEKP  DGNPGGAL SNLS DMSLDGW+GV+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADF+ NRNA+FDGSIS Q+RQ FR+MWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQ+ SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           FLMRLNFNSFFEATARGVLNVVR RPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 755

BLAST of CmaCh14G009040 vs. ExPASy TrEMBL
Match: A0A5A7VFR5 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G00280 PE=3 SV=1)

HSP 1 Score: 1383.2 bits (3579), Expect = 0.0e+00
Identity = 699/755 (92.58%), Postives = 722/755 (95.63%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIIDEREH+N LGLN+L  DAPISGEPTFKLA DISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRS + SSL  S ESSKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLAISTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQL+ EIERDDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLICEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHS+MPRLPL +KG  RNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNARNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMP FGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDPKEKPYTDGNPGGAL-SNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGD KEKP  DGNPGGAL SNLS DMS DGW+GV+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSPDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADF+  RNA+FDGSIS Q+RQ FR+MWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANIRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQ+ SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           FLMRLNFNSFFEATARGVLNVVR RP+ALPVLNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPSALPVLNQQ 755

BLAST of CmaCh14G009040 vs. ExPASy TrEMBL
Match: A0A6J1DP06 (Gamma-tubulin complex component OS=Momordica charantia OX=3673 GN=LOC111022444 PE=3 SV=1)

HSP 1 Score: 1376.3 bits (3561), Expect = 0.0e+00
Identity = 693/754 (91.91%), Postives = 719/754 (95.36%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIIDEREH+N LGLNHLPPDAPISGE TFKLA DISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLPPDAPISGESTFKLASDISFLEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRS +GSSL  S ESSK KTE PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSSLAKSAESSKAKTENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQD HGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDNHGEFFIRRQDDRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTD+SLTDWHLGFHIYLDMLPDYIHM VAESILFAGKAIRVLRN
Sbjct: 241 EQGSSVQDMSEKLGRLSTDDSLTDWHLGFHIYLDMLPDYIHMWVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAG QSHS++ R+PL IKG TRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGYQSHSNISRVPLNIKGNTRNFPLQKEPFIATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKE+SEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKEASEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTS+ED+YFSRVSLRMP FGV+VKS 
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVRVKST 480

Query: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           Q    EK YTDGNPG ALSNLSSDMSLDGW+GVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 Q----EKTYTDGNPGAALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF++NR A+ DGS+S  QRQ FR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFAQNRRAQRDGSVSSHQRQRFRRMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFT+LVG+HQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTDLVGYHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQ+ SS+PSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDSSSNPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVVR RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRARPAALPVLNQQ 750

BLAST of CmaCh14G009040 vs. NCBI nr
Match: XP_022984034.1 (gamma-tubulin complex component 4 homolog [Cucurbita maxima])

HSP 1 Score: 1504.2 bits (3893), Expect = 0.0e+00
Identity = 754/754 (100.00%), Postives = 754/754 (100.00%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI
Sbjct: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA
Sbjct: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480

Query: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 754

BLAST of CmaCh14G009040 vs. NCBI nr
Match: XP_022934806.1 (gamma-tubulin complex component 4 homolog [Cucurbita moschata] >XP_022934807.1 gamma-tubulin complex component 4 homolog [Cucurbita moschata])

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 738/754 (97.88%), Postives = 744/754 (98.67%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIIDEREH+NLLGLNHLPPDAPISGEPTFKLAPDISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRSS+GSSLPNSVESSKDKTEIPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHSHMPRLPLKIKG TRNFPLQKEPFFATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMP FGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGDPKEK YTDGNPGGALSNLSSDMSLDGW+ VALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKTYTDGNPGGALSNLSSDMSLDGWDSVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQ+RQ FRQMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQKRQRFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQ+KSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVV  RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of CmaCh14G009040 vs. NCBI nr
Match: XP_023528229.1 (gamma-tubulin complex component 4 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1464.5 bits (3790), Expect = 0.0e+00
Identity = 736/754 (97.61%), Postives = 743/754 (98.54%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIIDEREH+NLLGLNHLPPDAPISGEPTFKLAPDISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRI+V GFYYRELDRFATKSRNLSWIRSS+GSSLPNSVESSKDKTEIPSVYRRAIANGI
Sbjct: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHSHMPRLPLKIKG TRNFPLQKEPFFATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMP FGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGDPKEK YTDGNPGGALSN+SSDMSLDGW+GVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKTYTDGNPGGALSNISSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFSKNRN RFDGSISPQ+RQ FRQMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQ+KSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVV  RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of CmaCh14G009040 vs. NCBI nr
Match: KAG6581271.1 (Gamma-tubulin complex component 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1458.7 bits (3775), Expect = 0.0e+00
Identity = 735/754 (97.48%), Postives = 741/754 (98.28%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLGYTGDLIIDEREH+NLLGLNHLPPDAPISGEPTFKLAPDISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRSS+GSSLPNSVESSKDKTEIPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ AGNQSHSHMPRLPLKIKG TRNFPLQKEPFFATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQAAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRM  FGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMSSFGVKVKSA 480

Query: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGDPKEK YTDGNP GALSNLSSDMSLDGW+GVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKTYTDGNPRGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQ+RQ FRQMW VREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQKRQRFRQMWHVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQ+KSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVV  RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of CmaCh14G009040 vs. NCBI nr
Match: XP_038879485.1 (gamma-tubulin complex component 4 homolog [Benincasa hispida])

HSP 1 Score: 1413.7 bits (3658), Expect = 0.0e+00
Identity = 708/754 (93.90%), Postives = 728/754 (96.55%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLL+LLGYTGDLIIDEREH+N LGLNHLP DAPISGEPTF LAPDISFLE SERDL
Sbjct: 1   MLHELLLSLLGYTGDLIIDEREHYNSLGLNHLPLDAPISGEPTFTLAPDISFLEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRS +GSSL NS ESSKDK E PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILAIVTQGLDKF+VLFPPLHQL+LEIE DDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFYVLFPPLHQLILEIEHDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240

Query: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHI LDMLPDYIHM+VAESILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDVSEKLGRLSTDESLTDWHLGFHICLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360
           PSNAFWCQGAGNQSHSHMPRLPL IKG TRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEF KRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFRKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTS+EDRYFSRVSLRMP FGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGDPKEKP+ DGNPGGALSNL  DMSLDGW+GVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKPFIDGNPGGALSNLPLDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF+ NRNA+FDGSISPQQRQ FR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISPQQRQRFRRMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWS+ENQ+ SSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSLENQDGSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 755
           LMRLNFNSFFEATARGVLNVVR RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754

BLAST of CmaCh14G009040 vs. TAIR 10
Match: AT3G53760.1 (GAMMA-TUBULIN COMPLEX PROTEIN 4 )

HSP 1 Score: 1048.1 bits (2709), Expect = 3.3e-306
Identity = 550/750 (73.33%), Postives = 622/750 (82.93%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60
           MLHELLLALLG+TGDLI+DERE    LGL     D+P+S E TFKLAPDISF+E SERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGL-AFNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRS-SHGSSLPNSVESSKDKTE-IPSVYRRAIAN 120
           I+R++ LGFYYRELDRFA KSRNLSWIRS +    L  + E SK   E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGG 180
           GI EILSVYRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL++++LEIERDDIRGG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQVMYNQLA+WMVYGILQD HGEFFI+RQDD 
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETDQGSAVQVLSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRV 300
           + D  S+ + +SEKL R S  E SLTDWH GFHI LDMLPDYI M++ ESILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADK 360
           LRNPS AF  Q   ++S     R   +I+G   +   + E      LTG ELL QSEADK
Sbjct: 301 LRNPSPAF--QFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGELLPQSEADK 360

Query: 361 IEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKG 420
           IEAML DLKESSEFHKRSFE  +DS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL KG
Sbjct: 361 IEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEKG 420

Query: 421 DFFQCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKV 480
           DFFQCFLEES  LMRLPPRQST E+DLM+PFQLAATKT ++ED+YFSRVSLRMP FGV V
Sbjct: 421 DFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVTV 480

Query: 481 KSAQGD-PKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYC 540
           +S+Q D  + K    G      +NL+SD S+DGW+ +ALEY +DWP+QLFFTQEVLSKY 
Sbjct: 481 RSSQADMVRSKVSLTGK-----ANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSKYL 540

Query: 541 RVFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREH 600
           +VFQYL+RLKRTQMELEKSWAS+MHQDH + +++R    +GS S Q+RQ  R MWRVREH
Sbjct: 541 KVFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVREH 600

Query: 601 MAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVS 660
           MAFLIRNLQFYIQVDVIESQW +LQ  I DS DFTELVGFHQEYLSALISQSFLDIGSVS
Sbjct: 601 MAFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGSVS 660

Query: 661 RILDGIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPF 720
           RILD IMKLCLQFCW+IENQ  + + SELE++ EEFNKKSNSLYTILRSS+L GSQRAPF
Sbjct: 661 RILDSIMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRAPF 720

Query: 721 LRRFLMRLNFNSFFEATARGVLNVVRTRPA 747
           LRRFL+RLNFNSF+EATARGVLNVVR RPA
Sbjct: 721 LRRFLLRLNFNSFYEATARGVLNVVRQRPA 742

BLAST of CmaCh14G009040 vs. TAIR 10
Match: AT5G06680.1 (spindle pole body component 98 )

HSP 1 Score: 111.7 bits (278), Expect = 2.6e-24
Identity = 128/529 (24.20%), Postives = 211/529 (39.89%), Query Frame = 0

Query: 175 IRGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRR 234
           +RGG +   +H     G P +   +  LL      ++  + SW++ G L+D  GEFF+  
Sbjct: 334 LRGGAMAGAIHLHAQHGDPLVHDFMMSLLRCVCSPLFEMVRSWVLEGELEDTFGEFFV-- 393

Query: 235 QDDRETDQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKA 294
                  Q   V +L               W  G+ ++  MLP +I   +A+ IL  GK+
Sbjct: 394 -----VGQPVKVDLL---------------WREGYKLHPAMLPSFISPSLAQRILRTGKS 453

Query: 295 IRVLRNPSNAF-WCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQS 354
           I  LR   +   W   A   + +          GTT            T+  G   L   
Sbjct: 454 INFLRVCCDDHGWADAASEAAAA---------SGTT------------TRRGG---LGYG 513

Query: 355 EADKIEAMLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFL 414
           E D +E ++      +E  KR     ID        HL  ++  R     H  A+K Y L
Sbjct: 514 ETDALEHLV------TEAAKR-----ID-------KHLLDVLYKRYKFKEHCLAIKRYLL 573

Query: 415 LAKGDFFQCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLF 474
           L +GDF Q       +LM +   + +  A+ +  F+LA     +                
Sbjct: 574 LGQGDFVQ-------YLMDIVGPKLSEPANNISSFELAGFLEAA---------------- 633

Query: 475 GVKVKSAQGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLS 534
            ++  +AQ D  ++   D      + + S D    GW+  +LEY    PL   FT+ VLS
Sbjct: 634 -IRASNAQYD--DRDMLDRLRVKMMPHGSGDR---GWDVFSLEYEARVPLDTVFTESVLS 693

Query: 535 KYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRV 594
           KY RVF +L +LKR +  L   W ++        + N   +   S+  Q     R+   +
Sbjct: 694 KYLRVFNFLWKLKRVEHALIGIWKTMKPN---CITSNSFVKLQSSVKLQLLSALRRCQVL 753

Query: 595 REHMAFLIRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIG 654
              M   + N Q+YI  +V+E  W+     ++ + D  +L+  H++YL+A++ +S L   
Sbjct: 754 WNEMNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLLGEQ 766

Query: 655 S--------------------VSRILDGIMKLCLQFCWSIENQNKSSDP 683
           S                      R+ +GI +L ++   S   +NKS +P
Sbjct: 814 SQTIRESLFVLFELILRFRSHADRLYEGIHELQIRSKESGREKNKSQEP 766

BLAST of CmaCh14G009040 vs. TAIR 10
Match: AT5G17410.2 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 85.9 bits (211), Expect = 1.5e-16
Identity = 83/356 (23.31%), Postives = 148/356 (41.57%), Query Frame = 0

Query: 380 IDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQCFLEESCHLMRLPPRQST 439
           I +    A+  L  L+  + DL G L+++K Y LL +GDF   F++ +   +     + +
Sbjct: 342 IKAAHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEIS 401

Query: 440 AEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSAQGDPKEKPYTDGNPGGALS 499
            E  L     LA   T +  D       L   +    + +  G  K+   TD N      
Sbjct: 402 VE-KLQSLLDLALRTTAAAADP--RHEDLTCCVDRASLLTTLGMHKD---TDSN------ 461

Query: 500 NLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASL 559
           ++   MS+ G    +L Y + WPL +  +++ LSKY  +F++L   K  + +L  +W   
Sbjct: 462 SIEDPMSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAWQ-- 521

Query: 560 MHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFLIRNLQFYIQVDVIESQWNI 619
           +HQ     +    A    S+             +   M   I +L  Y+  +V+E  W++
Sbjct: 522 IHQGIRSMNSKGTAILRSSL-------------LCRSMLKFISSLLHYLTFEVLEPNWHV 581

Query: 620 LQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILDGIMKLCLQFC----WSIEN 679
           + DR+Q +    E++  H  +L   +    L +  V + ++ +  +CLQ+     W I +
Sbjct: 582 MHDRLQSTRSVDEVIQHHDFFLDKCLRGCLLLLPDVLKKMEKLKSVCLQYAAATQWLISS 641

Query: 680 Q---NKSSDPSE-----------LEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFL 718
               N  S P +           + +   EFN +  SL  +L      GSQ  P+L
Sbjct: 642 SIDINSQSHPQKTMIRDTTVTESIFNFEREFNSELQSLGPVLSK----GSQAEPYL 666

BLAST of CmaCh14G009040 vs. TAIR 10
Match: AT5G17410.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 85.9 bits (211), Expect = 1.5e-16
Identity = 83/356 (23.31%), Postives = 148/356 (41.57%), Query Frame = 0

Query: 380 IDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQCFLEESCHLMRLPPRQST 439
           I +    A+  L  L+  + DL G L+++K Y LL +GDF   F++ +   +     + +
Sbjct: 341 IKAAHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEIS 400

Query: 440 AEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSAQGDPKEKPYTDGNPGGALS 499
            E  L     LA   T +  D       L   +    + +  G  K+   TD N      
Sbjct: 401 VE-KLQSLLDLALRTTAAAADP--RHEDLTCCVDRASLLTTLGMHKD---TDSN------ 460

Query: 500 NLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASL 559
           ++   MS+ G    +L Y + WPL +  +++ LSKY  +F++L   K  + +L  +W   
Sbjct: 461 SIEDPMSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAWQ-- 520

Query: 560 MHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFLIRNLQFYIQVDVIESQWNI 619
           +HQ     +    A    S+             +   M   I +L  Y+  +V+E  W++
Sbjct: 521 IHQGIRSMNSKGTAILRSSL-------------LCRSMLKFISSLLHYLTFEVLEPNWHV 580

Query: 620 LQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILDGIMKLCLQFC----WSIEN 679
           + DR+Q +    E++  H  +L   +    L +  V + ++ +  +CLQ+     W I +
Sbjct: 581 MHDRLQSTRSVDEVIQHHDFFLDKCLRGCLLLLPDVLKKMEKLKSVCLQYAAATQWLISS 640

Query: 680 Q---NKSSDPSE-----------LEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFL 718
               N  S P +           + +   EFN +  SL  +L      GSQ  P+L
Sbjct: 641 SIDINSQSHPQKTMIRDTTVTESIFNFEREFNSELQSLGPVLSK----GSQAEPYL 665

BLAST of CmaCh14G009040 vs. TAIR 10
Match: AT3G43610.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 55.5 bits (132), Expect = 2.2e-07
Identity = 72/347 (20.75%), Postives = 132/347 (38.04%), Query Frame = 0

Query: 401  LNGHLKALKDYFLLAKGDFFQCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDED 460
            L  HL AL+ Y  +   D+   F+    H   L        A++    + +  +++ + D
Sbjct: 879  LQEHLLALRRYHFMELADWADVFVVSLWHHKWLVTEADKRIAEIQGFLESSIQRSSCERD 938

Query: 461  RYFSRVSLRMPLFGVKVKSAQGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYID 520
                R+ L            QG     P T G              +  ++ + L Y +D
Sbjct: 939  ICKDRIFL---------YKRQGTMHIPPSTIG--------------VRSFDFLRLGYRVD 998

Query: 521  WPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSIS 580
            WP+ +  T + L+ Y  VF +L+++K     L   W SL    H    K         I 
Sbjct: 999  WPISIILTCDALTAYADVFSFLVQVKLAAYVLTDVWCSLKDVRHMMHEKKE------KIL 1058

Query: 581  PQQRQHFRQMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDRIQDS-HDFTELVGFHQE 640
             Q+ +    + ++R  +   +  LQ Y+  ++    W+     +++   D  +L   H  
Sbjct: 1059 KQELRWLNILMKLRHQVNHFVTALQQYVHSELSHVSWSKFLHSLKNKVKDMMDLESVHMA 1118

Query: 641  YLSALISQSFL--DIGSVSRILDGIMKLCLQFCWSIENQNKSSD--------------PS 700
            YLS  +   FL  +   +S I++ I++  L F   +    +S+D               S
Sbjct: 1119 YLSEALRICFLSDETQIISNIIENILQCALDFRSCLPRGIQSTDRVPNDSWTKTLGINTS 1178

Query: 701  ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRFLMRLNFNSFF 731
            ++  + + F+K+   L+     S   G      L RF   LNFN ++
Sbjct: 1179 QVMMVKQNFDKELKELHKCHLRSPKHGKYG---LSRFWDYLNFNLYY 1193