CmaCh14G008610 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G008610
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRING/FYVE/PHD zinc finger superfamily protein
LocationCma_Chr14: 4434862 .. 4439067 (+)
RNA-Seq ExpressionCmaCh14G008610
SyntenyCmaCh14G008610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTATCGCACTAATTCTATTCCCATTTTCGTAAGCATCCTTGTTTCCTCTTTTTCTCTTGCATTTAATCTTAGTTCCTATGCTGTTCATAAGTTTTTGTCTGCAATGTGTTTGTTCATTTGCTAATCATGTTGGTAGCTGTTTGAAGGTTGCTGCCATTCTCTCTTTTTGTGCATTTTCGTTAATTTCAGGTTTTTCTCGATCTGGATTGCTGTTTTCTTGAATTTCTTCGGGTTTGGTTTCTGAGTTTTCTCTTTGTTTGGTTTATACTATTTAATCATATTGATTTAGTCTTGCTTGGTTTTTGATGTTTTGAGTTGTCATTTTCTCTTACATTCCTACATTTTTGCAAGATTTTTGTAACATCTTTCGTTCGATTGCTGCCAAAACTCGGTTAGTTTAACTATGAATAATGAGTTCAAACGTCTGAATCTGTTCAGAGGTCTTAGAACTAAGTTCTGGGGCAAAATCCTTGCAGTTTGTTCATAGTCCATAGGGCTCTTCCTACTAATTCTTGAGGAGGCTTTGCCTGTTAGAAGACCAATCTCCAGGGATGATAAGTATTTCAGAGTATTAAGTTTTCAAAATCAAGGTTTGAACCTTTAACTAAATCGAGTTGACTAAATTGTGCTTGGAGCATTGATGTAACTTTTTGAGTATCAGTGAAATGTCTCTTTTTTTCCTCCTAAAGAAAGACAAAAAGGATAACTGTAAGGAGCGTTTGGTTTTTCATGGCTCGTTTAACATTCAAATTTTTGTTCCCTTTCTCTTTTCTCCTGCAACTTATGATTATTTATTTATAGAATAATTATTATTACATTTGTCTAATCATCAGGTGAATTCAATGATTCTTTCCTCTTCTTTTTATTTTTATTTTTAATTTTTAAAGAACTGGGGAATCAGGATTTCATTCCTTGCATACTCATGGCCCACTTCATAGATCTTCGAGAAGCTTTGCCCACTCCGAAGCCAACTTTAGAAAACAACAAGAATTTCAAATTAGTAATTTTTTTACCTAGGAATCGGAATTGAACTCATGAGAAGAGGATATTTGTAAGAGCCTAAGCCTACCACTAGCAGATATTGTCCTCTTTGGGCTTTCCCTTTCGGGTTTCCCCTCTAGATTTTTAAAACGCGTTTACTAGGGAGAGGTTTTCACACCCTTATAAGGAATGCTTCGTTCTCCTCCCCAACCAATGTGGGATCTCACAATCCACCCCCCTTTGGGGCCCAGCGTCCTTGTTAACACCCGTTCCCTTCTCCAATCGATTTAATGTGGGACCCCTCAATCCACCCCCCTTCAAGGTCCAGCGTCCTTGTTGGCATACCGCTTTCTGTCCACCCCTCTTCGGGGCTCAGCCTCCTCGCTAGTATATCACCCGGTGTATGGCTCTGATACCATTTGTAACAGCCCAAACCCACCGCTAGCAGATATTGTCCTATTTGGACTTTCCCTTTCGGGCTTCCCTTCAAGGTTTTTAAAACGCGTCTACTAGGGAGAGGTTTCCACACCCTTATAAAGATTGCTTCGTTTTTCTCCCCAACCGACATGGAATCTCACAATATCTTATGATATCCCAATTCTAAGGACCTTACTAGTCACACCATCGCCCCATGGGTTGTAAACTCCAAAAGTTTCAAAATTTCTATTCTTTCTTTATTTTCTGCTAGATCTTTGAATTATACTATGTTGAGAAACAATCTGAATTGTCATGGATGTTGTTCACTGCAGCAAACAAAATTGATCTACATAGTTGTAAATGTTGTTTGGCTTATTTTATATGATTTTTTTGACATTCATTCATTCCAATGTTTACTGGGTCATTGAAGAATGGGGGAACATTTTGTGCTGTTGGTGGATCAGTTGCTCACAGAATCAAACCTTGAAGCTACTATTGAGAGAAAAAACCGAAATTGTCGTCCTATGGCCTCAACAAGTGCAGATAACATGATATCTTCCTTAAATATTGATGTTGAATTCATGCCATCTTCAAGTAAACTTGTTCAGTGCAGAATTTGTCATGATGAGGACGATGAATCAAAAATGGAGGCACCATGCTCTTGCTGTGGAAGCTTGAAGGTTAGATTTGGTATACTCAACACCAATTAGTGCTACTTAATCAATAAAACTCAGATAGCTTTTCTTTGAATCCCTCGTTTCCAAATTATGACTGACTAAACTGGTTTCAACAATGTTCCTGTTGATATTTTCTGCACGTTTCCAAATTATATGACTGACTAAACTGGTTTCAACAATGTTCTTATCGATATTTTCTGCATTTAGTTTCAGTCCAACTTTATATTTTCCCCCAAATCTTTGCCCCGGATGTTATAATATGCTATAAACACTTGCTAAGGCAGGTCCCTTGGTTTATATTTACTTGTTGCTAGACTGAAAATGTTAGAATCAGATTCATATCTTTATTCCTCACTGTGTTCGTTTAGATGGTTCACCTTGATAATTAGCTTCCAGTTAGTGTTGATAATTACAGTATGCTCATCGCAAGTGCGTTCAGCGGTGGTGCAACGAGAAAGGCAATACAATCTGTGAAATTTGCCACCAGGTCTGTTGTTTTTGTTCAAATAAATGAAAAATTCTTTAATACATAACTAACTTTATGGTCGTAAGAAGTCGTTTTTCTTGTACTGCAGGATTTTGAGCCAGGGTATACAGCACCACTCCCTGTATTATATTATGGAGATATTACTTCACCAGTTCATTTCAGGTTAACCATTTACCTCTCTCCCCTCAATGCAAGTTTCTCTAGTTTGCATTGTATATTCACTTATTGTTCGTTTATTTTTTAGACACTATTGATATAATATCACTGTCGTCGAATTGTGTTCTTAGGGAAAGTTGGGAGATGTCTAGACTGAACCTGCATGTACCTGCAGGAATGCCTGATCACGAGTATCCTGATTCTGACTTTGATGAATTCTTCACTCCTTCTCCAAGAAGCATATTGTGCTGCCGCATGGTTGCAGTAACTGTATTCCCTACCTCTTCATTTTACTTCATTTCCAGTTTCCTTATCTCTGTAAACAAATTTAAATGGATTGATAATTGGCTTTGCCTTCTGCACATAAAAGAAGTATTAAATGTTCTTGCTCGTTGATTCTTTTTCATTCACTTGTGAAGGGAGCAAAGCAAACATAGTTTTTGGTTTAAGTTCGAACATTCGTTCTTATTTTAGTCTTTGAACTTTCTATTAAGACTTTGAACATTCGTTCTTCTGCATGAACAAGTGTTTGATTCACTATTAGTAAGTTAAAGTATTGGGTCTTCAAGCATCAACGAAAAGAATTTAAGTAGTCATGTAATCTAAGTTTCGGAGACGAAATAGAGTATTAGAAGTCGATGGATTAAAATATAACGTTTTGAAGCTTAAGAATGAAAATGAAACAAGCATCAAAGTTCATATCTATAATACGATTTAAACCTGTATTCGCCTTGATTATATTTGACCAACAATCCCGAGTTTGAAACTTCTAATCGTATCTGTATGTTGCAGTTTATCGTTCTTCTCGTTTTGCGCCATACACTTCCAATTGTAATCAGTGGAGCTGGAGAGTATTCATTGACATTGTTAATGGTAGGCTTGTTTTCTCTGTTTCCCCTTCTACTTCTCCTTCCTTTGGCCCTCTTCCATTGTTGAGACCAATGAAGAACCATAAGTTCATGTGCTGTATGTTTCAGCTGCTGATCTTGAGAATTGTTGGAATCCTTCTACCAATCTATGTCATGGTTAGAGCATTTACTTCCATTCAACGACGGCGTCATTATCAGGTTAGTGATGCTGTTTATGCCTGGATTATTCGTTTCATCGTAAGAACTGCTTGGATTTGTGAAGAGGTCGATGCAGAATAGACATGAATTTTGTTGGGCTATTCTGTTATACGGTGCCTCATATGTAGTTAAAGTGCATTTAAGGACATTGTTGATCAACTTGAACGTAACTCAACTAATTAAAGCATATATTACCAACCATGATGAACATACAAGAGAGAGTGCACATTCCTGAAAATTTGCTCTGGTTATCTATTATCGTTGAGCTTGATGTCTAGCAGTTGTTTACATTCCCTTGATACGACGTTTGAGTATACTAGTATGCTTTTGGTAGGATCCCCGTCTTCGCCTCGCGACACCAGAGGACGAAAATGACTCGACAAACCATGCTCAATCGCGCTTCATCCACGTGAGATAG

mRNA sequence

CTTCTATCGCACTAATTCTATTCCCATTTTCAATGGGGGAACATTTTGTGCTGTTGGTGGATCAGTTGCTCACAGAATCAAACCTTGAAGCTACTATTGAGAGAAAAAACCGAAATTGTCGTCCTATGGCCTCAACAAGTGCAGATAACATGATATCTTCCTTAAATATTGATGTTGAATTCATGCCATCTTCAAGTAAACTTGTTCAGTGCAGAATTTGTCATGATGAGGACGATGAATCAAAAATGGAGGCACCATGCTCTTGCTGTGGAAGCTTGAAGTATGCTCATCGCAAGTGCGTTCAGCGGTGGTGCAACGAGAAAGGCAATACAATCTGTGAAATTTGCCACCAGGATTTTGAGCCAGGGTATACAGCACCACTCCCTGTATTATATTATGGAGATATTACTTCACCAGTTCATTTCAGGGAAAGTTGGGAGATGTCTAGACTGAACCTGCATGTACCTGCAGGAATGCCTGATCACGAGTATCCTGATTCTGACTTTGATGAATTCTTCACTCCTTCTCCAAGAAGCATATTGTGCTGCCGCATGGTTGCAGTAACTTTTATCGTTCTTCTCGTTTTGCGCCATACACTTCCAATTGTAATCAGTGGAGCTGGAGAGTATTCATTGACATTGTTAATGCTGCTGATCTTGAGAATTGTTGGAATCCTTCTACCAATCTATGTCATGGTTAGAGCATTTACTTCCATTCAACGACGGCGTCATTATCAGGATCCCCGTCTTCGCCTCGCGACACCAGAGGACGAAAATGACTCGACAAACCATGCTCAATCGCGCTTCATCCACGTGAGATAG

Coding sequence (CDS)

ATGGGGGAACATTTTGTGCTGTTGGTGGATCAGTTGCTCACAGAATCAAACCTTGAAGCTACTATTGAGAGAAAAAACCGAAATTGTCGTCCTATGGCCTCAACAAGTGCAGATAACATGATATCTTCCTTAAATATTGATGTTGAATTCATGCCATCTTCAAGTAAACTTGTTCAGTGCAGAATTTGTCATGATGAGGACGATGAATCAAAAATGGAGGCACCATGCTCTTGCTGTGGAAGCTTGAAGTATGCTCATCGCAAGTGCGTTCAGCGGTGGTGCAACGAGAAAGGCAATACAATCTGTGAAATTTGCCACCAGGATTTTGAGCCAGGGTATACAGCACCACTCCCTGTATTATATTATGGAGATATTACTTCACCAGTTCATTTCAGGGAAAGTTGGGAGATGTCTAGACTGAACCTGCATGTACCTGCAGGAATGCCTGATCACGAGTATCCTGATTCTGACTTTGATGAATTCTTCACTCCTTCTCCAAGAAGCATATTGTGCTGCCGCATGGTTGCAGTAACTTTTATCGTTCTTCTCGTTTTGCGCCATACACTTCCAATTGTAATCAGTGGAGCTGGAGAGTATTCATTGACATTGTTAATGCTGCTGATCTTGAGAATTGTTGGAATCCTTCTACCAATCTATGTCATGGTTAGAGCATTTACTTCCATTCAACGACGGCGTCATTATCAGGATCCCCGTCTTCGCCTCGCGACACCAGAGGACGAAAATGACTCGACAAACCATGCTCAATCGCGCTTCATCCACGTGAGATAG

Protein sequence

MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQCRICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVLYYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLRLATPEDENDSTNHAQSRFIHVR
Homology
BLAST of CmaCh14G008610 vs. ExPASy Swiss-Prot
Match: Q5T0T0 (E3 ubiquitin-protein ligase MARCHF8 OS=Homo sapiens OX=9606 GN=MARCHF8 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.5e-08
Identity = 32/77 (41.56%), Postives = 42/77 (54.55%), Query Frame = 0

Query: 34  STSADNMISSLNIDVEFMPSSSKLVQCRICHDE-DDESKMEAPCSCCGSLKYAHRKCVQR 93
           S SA   +SS +      PSS  +  CRICH E DDES +  PC C GSL + H+ C+Q+
Sbjct: 57  SASAPAPVSSFS-RTSITPSSQDI--CRICHCEGDDESPLITPCHCTGSLHFVHQACLQQ 116

Query: 94  WCNEKGNTICEICHQDF 110
           W        CE+C  +F
Sbjct: 117 WIKSSDTRCCELCKYEF 130

BLAST of CmaCh14G008610 vs. ExPASy Swiss-Prot
Match: Q0VD59 (E3 ubiquitin-protein ligase MARCHF8 OS=Bos taurus OX=9913 GN=MARCHF8 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.4e-08
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 0

Query: 60  CRICHDE-DDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDF 110
           CRICH E DDES +  PC C GSL + H+ C+Q+W        CE+C  +F
Sbjct: 78  CRICHCEGDDESPLITPCRCTGSLHFVHQTCLQQWIKSSDTRCCELCKYEF 128

BLAST of CmaCh14G008610 vs. ExPASy Swiss-Prot
Match: Q9DBD2 (E3 ubiquitin-protein ligase MARCHF8 OS=Mus musculus OX=10090 GN=Marchf8 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.4e-08
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 0

Query: 60  CRICHDE-DDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDF 110
           CRICH E DDES +  PC C GSL + H+ C+Q+W        CE+C  +F
Sbjct: 76  CRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYEF 126

BLAST of CmaCh14G008610 vs. ExPASy Swiss-Prot
Match: Q5XH39 (E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus laevis OX=8355 GN=marchf8 PE=2 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 4.4e-08
Identity = 27/59 (45.76%), Postives = 35/59 (59.32%), Query Frame = 0

Query: 52  PSSSKLVQCRICHDE-DDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDF 110
           PSS  +  CRICH E DDES +  PC C GSL + H+ C+Q+W        CE+C  +F
Sbjct: 47  PSSQDI--CRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKFEF 103

BLAST of CmaCh14G008610 vs. ExPASy Swiss-Prot
Match: Q28IK8 (E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus tropicalis OX=8364 GN=marchf8 PE=2 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 5.7e-08
Identity = 35/100 (35.00%), Postives = 49/100 (49.00%), Query Frame = 0

Query: 11  QLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQCRICHDE-DDE 70
           +L  E  L  ++ R +   +  + TS     S     V   PSS  +  CRICH E DDE
Sbjct: 8   KLQNEKTLGHSVSRSSNISKAGSPTSVSAPSSFPRTSV--TPSSQDI--CRICHCEGDDE 67

Query: 71  SKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDF 110
           S +  PC C GSL + H+ C+Q+W        CE+C  +F
Sbjct: 68  SPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKFEF 103

BLAST of CmaCh14G008610 vs. ExPASy TrEMBL
Match: A0A6J1J9L9 (uncharacterized protein LOC111482515 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482515 PE=4 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.3e-148
Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180

Query: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240
           VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR
Sbjct: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240

Query: 241 LATPEDENDSTNHAQSRFIHVR 263
           LATPEDENDSTNHAQSRFIHVR
Sbjct: 241 LATPEDENDSTNHAQSRFIHVR 262

BLAST of CmaCh14G008610 vs. ExPASy TrEMBL
Match: A0A6J1J9N6 (uncharacterized protein LOC111482515 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482515 PE=4 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.2e-145
Identity = 258/262 (98.47%), Postives = 258/262 (98.47%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM    FI
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM----FI 180

Query: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240
           VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR
Sbjct: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240

Query: 241 LATPEDENDSTNHAQSRFIHVR 263
           LATPEDENDSTNHAQSRFIHVR
Sbjct: 241 LATPEDENDSTNHAQSRFIHVR 258

BLAST of CmaCh14G008610 vs. ExPASy TrEMBL
Match: A0A6J1FB67 (uncharacterized protein LOC111442434 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442434 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.3e-143
Identity = 254/262 (96.95%), Postives = 255/262 (97.33%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MGEHFVLLVDQLLTESNLE+TIERKNRNCRPMASTSADNMISSLNIDVEFM  SSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLESTIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKME PCSCCGSLKYAHR CVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRMCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180

Query: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240
           VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQ RRHYQDPRL 
Sbjct: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQGRRHYQDPRLH 240

Query: 241 LATPEDENDSTNHAQSRFIHVR 263
           LATPEDENDSTNHAQS FIHVR
Sbjct: 241 LATPEDENDSTNHAQSHFIHVR 262

BLAST of CmaCh14G008610 vs. ExPASy TrEMBL
Match: A0A6J1F655 (uncharacterized protein LOC111442434 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442434 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.0e-140
Identity = 250/262 (95.42%), Postives = 251/262 (95.80%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MGEHFVLLVDQLLTESNLE+TIERKNRNCRPMASTSADNMISSLNIDVEFM  SSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLESTIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKME PCSCCGSLKYAHR CVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRMCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM    FI
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM----FI 180

Query: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240
           VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQ RRHYQDPRL 
Sbjct: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQGRRHYQDPRLH 240

Query: 241 LATPEDENDSTNHAQSRFIHVR 263
           LATPEDENDSTNHAQS FIHVR
Sbjct: 241 LATPEDENDSTNHAQSHFIHVR 258

BLAST of CmaCh14G008610 vs. ExPASy TrEMBL
Match: A0A0A0LBM8 (RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G186720 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.1e-117
Identity = 215/264 (81.44%), Postives = 232/264 (87.88%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMAST--SADNMISSLNIDVEFMPSSSKLV 60
           MG+HF LLVDQLLTESNLEATIERKNR C PMAST  +ADNMISS NIDVE + S S +V
Sbjct: 1   MGDHFGLLVDQLLTESNLEATIERKNRICHPMASTNANADNMISSSNIDVESV-SPSSIV 60

Query: 61  QCRICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLP 120
           QCRICHDEDD SKME PCSCCGSLKYAHRKC+QRWCNEKG+TICEICHQDF+PGYT+P P
Sbjct: 61  QCRICHDEDDGSKMETPCSCCGSLKYAHRKCIQRWCNEKGDTICEICHQDFKPGYTSPPP 120

Query: 121 VLYYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVT 180
           V YYGDI SP+HFR SWEMSRLNLHVPAGMPDHEY DSDFD+FF PSPRSILCCR+VAV 
Sbjct: 121 VFYYGDINSPIHFRGSWEMSRLNLHVPAGMPDHEYLDSDFDDFFAPSPRSILCCRVVAVI 180

Query: 181 FIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPR 240
           FI LLVLRHTLPIVISGAG YS TLLMLLILRIVGILLPIYVMV+AFT IQRR  YQ PR
Sbjct: 181 FIALLVLRHTLPIVISGAGGYSWTLLMLLILRIVGILLPIYVMVKAFTYIQRRHRYQAPR 240

Query: 241 LRLATPEDENDSTNHAQSRFIHVR 263
           L+L T  D ++STNH+QS  IH+R
Sbjct: 241 LQLTTLGDNSESTNHSQSHLIHLR 263

BLAST of CmaCh14G008610 vs. NCBI nr
Match: XP_022984108.1 (uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984109.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984110.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984111.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984112.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984113.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984114.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984115.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984116.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984117.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984118.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984119.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984120.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984122.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984123.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984124.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984125.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984126.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984127.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984129.1 uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima])

HSP 1 Score: 535.4 bits (1378), Expect = 2.7e-148
Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180

Query: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240
           VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR
Sbjct: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240

Query: 241 LATPEDENDSTNHAQSRFIHVR 263
           LATPEDENDSTNHAQSRFIHVR
Sbjct: 241 LATPEDENDSTNHAQSRFIHVR 262

BLAST of CmaCh14G008610 vs. NCBI nr
Match: XP_023528859.1 (uncharacterized protein LOC111791660 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528860.1 uncharacterized protein LOC111791660 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528861.1 uncharacterized protein LOC111791660 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528.9 bits (1361), Expect = 2.5e-146
Identity = 259/262 (98.85%), Postives = 259/262 (98.85%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFM  SSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKME PCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180

Query: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240
           VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR
Sbjct: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240

Query: 241 LATPEDENDSTNHAQSRFIHVR 263
           LATPEDENDSTNHAQSRFIHVR
Sbjct: 241 LATPEDENDSTNHAQSRFIHVR 262

BLAST of CmaCh14G008610 vs. NCBI nr
Match: KAG7017957.1 (E3 ubiquitin-protein ligase MARCH8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 524.2 bits (1349), Expect = 6.3e-145
Identity = 258/262 (98.47%), Postives = 258/262 (98.47%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFM  SSKLVQC
Sbjct: 54  MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 113

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKME PCSC GSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 114 RICHDEDDESKMETPCSCRGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 173

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI
Sbjct: 174 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 233

Query: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240
           VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR
Sbjct: 234 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 293

Query: 241 LATPEDENDSTNHAQSRFIHVR 263
           LATPEDENDSTNHAQSRFIHVR
Sbjct: 294 LATPEDENDSTNHAQSRFIHVR 315

BLAST of CmaCh14G008610 vs. NCBI nr
Match: XP_022984128.1 (uncharacterized protein LOC111482515 isoform X2 [Cucurbita maxima])

HSP 1 Score: 523.5 bits (1347), Expect = 1.1e-144
Identity = 258/262 (98.47%), Postives = 258/262 (98.47%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM    FI
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRM----FI 180

Query: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240
           VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR
Sbjct: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240

Query: 241 LATPEDENDSTNHAQSRFIHVR 263
           LATPEDENDSTNHAQSRFIHVR
Sbjct: 241 LATPEDENDSTNHAQSRFIHVR 258

BLAST of CmaCh14G008610 vs. NCBI nr
Match: XP_022935629.1 (uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935630.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935631.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935632.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935633.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935634.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935635.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935636.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935637.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935638.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935640.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935641.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_022935643.1 uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata])

HSP 1 Score: 518.8 bits (1335), Expect = 2.6e-143
Identity = 254/262 (96.95%), Postives = 255/262 (97.33%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MGEHFVLLVDQLLTESNLE+TIERKNRNCRPMASTSADNMISSLNIDVEFM  SSKLVQC
Sbjct: 1   MGEHFVLLVDQLLTESNLESTIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDEDDESKME PCSCCGSLKYAHR CVQRWCNEKGNTICEICHQDFEPGYTAPLPVL
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRMCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180
           YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTFI 180

Query: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRLR 240
           VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQ RRHYQDPRL 
Sbjct: 181 VLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQGRRHYQDPRLH 240

Query: 241 LATPEDENDSTNHAQSRFIHVR 263
           LATPEDENDSTNHAQS FIHVR
Sbjct: 241 LATPEDENDSTNHAQSHFIHVR 262

BLAST of CmaCh14G008610 vs. TAIR 10
Match: AT2G01275.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 212.2 bits (539), Expect = 4.9e-55
Identity = 119/270 (44.07%), Postives = 164/270 (60.74%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MG+HFVLLVD+L+TES +EA I+ +N+  +         ++    +++        + +C
Sbjct: 1   MGDHFVLLVDRLITESTIEAAIQSRNQMLQANIPVEECTILDDKTLEM-LRNGDLSMAEC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDED +S ME PCSC GS+KYAHR+CVQRWCNEKG+T CEICHQ+F+P YTAP P+L
Sbjct: 61  RICHDEDLDSNMETPCSCSGSVKYAHRRCVQRWCNEKGDTTCEICHQEFKPDYTAPPPLL 120

Query: 121 YYGDITSPVHFRESWEMS----RLNLHVPAGMP--DHEYPDSDFDEFFTPSPRSILCCRM 180
             G +  P+HFR +W +S    R    VPA     D  YP S        S  S +CCR 
Sbjct: 121 ELGHV--PLHFRGNWGISQREHRFITVVPADSTFIDQPYPLS--------STTSFICCRS 180

Query: 181 VAVTFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHY 240
           + + F+ LL+LRHTLP+V++G+  +   L  LL LRI+GI+LPIYV+ +A  + +R    
Sbjct: 181 LVLIFMALLILRHTLPLVLTGSNLHVFPLFTLLFLRILGIMLPIYVVTKAVATCRRHSRT 240

Query: 241 QDPRLRLATPEDENDSTN--HAQSRFIHVR 263
            D      + ++E DS      QS  I VR
Sbjct: 241 LDTSPSDDSSDEETDSWRLPQTQSYIIGVR 259

BLAST of CmaCh14G008610 vs. TAIR 10
Match: AT2G01275.2 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 212.2 bits (539), Expect = 4.9e-55
Identity = 119/270 (44.07%), Postives = 164/270 (60.74%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMASTSADNMISSLNIDVEFMPSSSKLVQC 60
           MG+HFVLLVD+L+TES +EA I+ +N+  +         ++    +++        + +C
Sbjct: 1   MGDHFVLLVDRLITESTIEAAIQSRNQMLQANIPVEECTILDDKTLEM-LRNGDLSMAEC 60

Query: 61  RICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120
           RICHDED +S ME PCSC GS+KYAHR+CVQRWCNEKG+T CEICHQ+F+P YTAP P+L
Sbjct: 61  RICHDEDLDSNMETPCSCSGSVKYAHRRCVQRWCNEKGDTTCEICHQEFKPDYTAPPPLL 120

Query: 121 YYGDITSPVHFRESWEMS----RLNLHVPAGMP--DHEYPDSDFDEFFTPSPRSILCCRM 180
             G +  P+HFR +W +S    R    VPA     D  YP S        S  S +CCR 
Sbjct: 121 ELGHV--PLHFRGNWGISQREHRFITVVPADSTFIDQPYPLS--------STTSFICCRS 180

Query: 181 VAVTFIVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHY 240
           + + F+ LL+LRHTLP+V++G+  +   L  LL LRI+GI+LPIYV+ +A  + +R    
Sbjct: 181 LVLIFMALLILRHTLPLVLTGSNLHVFPLFTLLFLRILGIMLPIYVVTKAVATCRRHSRT 240

Query: 241 QDPRLRLATPEDENDSTN--HAQSRFIHVR 263
            D      + ++E DS      QS  I VR
Sbjct: 241 LDTSPSDDSSDEETDSWRLPQTQSYIIGVR 259

BLAST of CmaCh14G008610 vs. TAIR 10
Match: AT5G38070.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.1e-53
Identity = 109/248 (43.95%), Postives = 164/248 (66.13%), Query Frame = 0

Query: 1   MGEHFVLLVDQLLTESNLEATIERKNRNCRPMA-STSADNMISSLNIDVEFMPSSSKLVQ 60
           MG+H VL+VD+L ++S L  T+ R +    P A S + D +  S++   +     SK VQ
Sbjct: 1   MGDHLVLVVDRLSSDSGL-GTVNRAD----PKADSVNEDGVSESISAGADL--CESKFVQ 60

Query: 61  CRICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLPV 120
           CRICHDED+++ M+ PCSC G+LK+AH  CVQRWCNEKG+T+CEIC Q ++PGYTAP  +
Sbjct: 61  CRICHDEDEDTNMDTPCSCSGTLKFAHHNCVQRWCNEKGDTVCEICRQQYKPGYTAPRQL 120

Query: 121 LYYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAVTF 180
            +Y  I+  ++F   W +  L+L  P  +   +  D D D +   SP S++CCR++A+ F
Sbjct: 121 FHYTGIS--MNFGSDWGIEGLDLRNPYFLTWGD-ADDDHDLYSFHSPTSLICCRLIALLF 180

Query: 181 IVLLVLRHTLPIVISGAGEYSLTLLMLLILRIVGILLPIYVMVRAFTSIQRRRHYQDPRL 240
           ++LL LRH+LP+++ G  ++S+TLLML ++R +GILL  YV  ++F  IQR R  +D RL
Sbjct: 181 VLLLFLRHSLPVLLGGVDDFSITLLMLPLVRTLGILLIAYVFFKSFIVIQRCRQERDTRL 238

Query: 241 RLATPEDE 248
              + ++E
Sbjct: 241 SGFSSDEE 238

BLAST of CmaCh14G008610 vs. TAIR 10
Match: AT2G02960.2 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 2.4e-38
Identity = 83/184 (45.11%), Postives = 110/184 (59.78%), Query Frame = 0

Query: 59  QCRICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLP 118
           +CRIC DE     +E+PC+C GSLKYAHRKCVQRWCNEKGN ICEICHQ ++PGYTAP P
Sbjct: 42  ECRICSDESPVENLESPCACSGSLKYAHRKCVQRWCNEKGNIICEICHQPYQPGYTAPPP 101

Query: 119 VLYYGDITSPVHFRESWEMSRLNLHVP----AGMPDHEYPDSDFDEFFTPSPRSILCCRM 178
            L   + T  +     W +S L++H P        +  Y +S++ E+   S      CR 
Sbjct: 102 PLQPEETT--IDIGGGWTISGLDVHDPRLLAIAEAERRYLESEYVEYTASSASGAAFCRS 161

Query: 179 VAVTFIVLLVLRHTLPIVISGAGEYS--LTLLMLLILRIVGILLPIYVMVRAFTSIQRRR 237
            A+  + LL+LRH L I     GE     ++L L++LR  G LLP Y+M  A + +QRRR
Sbjct: 162 AALILMALLLLRHALTITDDTDGEEDDPSSILSLVLLRAAGFLLPCYIMAWAISILQRRR 221

BLAST of CmaCh14G008610 vs. TAIR 10
Match: AT2G02960.4 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 2.4e-38
Identity = 83/184 (45.11%), Postives = 110/184 (59.78%), Query Frame = 0

Query: 59  QCRICHDEDDESKMEAPCSCCGSLKYAHRKCVQRWCNEKGNTICEICHQDFEPGYTAPLP 118
           +CRIC DE     +E+PC+C GSLKYAHRKCVQRWCNEKGN ICEICHQ ++PGYTAP P
Sbjct: 42  ECRICSDESPVENLESPCACSGSLKYAHRKCVQRWCNEKGNIICEICHQPYQPGYTAPPP 101

Query: 119 VLYYGDITSPVHFRESWEMSRLNLHVP----AGMPDHEYPDSDFDEFFTPSPRSILCCRM 178
            L   + T  +     W +S L++H P        +  Y +S++ E+   S      CR 
Sbjct: 102 PLQPEETT--IDIGGGWTISGLDVHDPRLLAIAEAERRYLESEYVEYTASSASGAAFCRS 161

Query: 179 VAVTFIVLLVLRHTLPIVISGAGEYS--LTLLMLLILRIVGILLPIYVMVRAFTSIQRRR 237
            A+  + LL+LRH L I     GE     ++L L++LR  G LLP Y+M  A + +QRRR
Sbjct: 162 AALILMALLLLRHALTITDDTDGEEDDPSSILSLVLLRAAGFLLPCYIMAWAISILQRRR 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5T0T01.5e-0841.56E3 ubiquitin-protein ligase MARCHF8 OS=Homo sapiens OX=9606 GN=MARCHF8 PE=1 SV=1[more]
Q0VD594.4e-0847.06E3 ubiquitin-protein ligase MARCHF8 OS=Bos taurus OX=9913 GN=MARCHF8 PE=2 SV=1[more]
Q9DBD24.4e-0847.06E3 ubiquitin-protein ligase MARCHF8 OS=Mus musculus OX=10090 GN=Marchf8 PE=2 SV=... [more]
Q5XH394.4e-0845.76E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus laevis OX=8355 GN=marchf8 PE=2 SV... [more]
Q28IK85.7e-0835.00E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus tropicalis OX=8364 GN=marchf8 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1J9L91.3e-148100.00uncharacterized protein LOC111482515 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1J9N65.2e-14598.47uncharacterized protein LOC111482515 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FB671.3e-14396.95uncharacterized protein LOC111442434 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1F6555.0e-14095.42uncharacterized protein LOC111442434 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0LBM82.1e-11781.44RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G18672... [more]
Match NameE-valueIdentityDescription
XP_022984108.12.7e-148100.00uncharacterized protein LOC111482515 isoform X1 [Cucurbita maxima] >XP_022984109... [more]
XP_023528859.12.5e-14698.85uncharacterized protein LOC111791660 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
KAG7017957.16.3e-14598.47E3 ubiquitin-protein ligase MARCH8, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022984128.11.1e-14498.47uncharacterized protein LOC111482515 isoform X2 [Cucurbita maxima][more]
XP_022935629.12.6e-14396.95uncharacterized protein LOC111442434 isoform X1 [Cucurbita moschata] >XP_0229356... [more]
Match NameE-valueIdentityDescription
AT2G01275.14.9e-5544.07RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G01275.24.9e-5544.07RING/FYVE/PHD zinc finger superfamily protein [more]
AT5G38070.12.1e-5343.95RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G02960.22.4e-3845.11RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G02960.42.4e-3845.11RING/FYVE/PHD zinc finger superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 59..106
e-value: 5.6E-20
score: 82.4
IPR011016Zinc finger, RING-CH-typePFAMPF12906RINGvcoord: 60..105
e-value: 3.8E-12
score: 46.1
IPR011016Zinc finger, RING-CH-typePROSITEPS51292ZF_RING_CHcoord: 52..112
score: 21.907717
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 49..114
e-value: 1.6E-19
score: 71.2
IPR022143Protein of unknown function DUF3675PFAMPF12428DUF3675coord: 111..224
e-value: 2.1E-33
score: 115.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 239..262
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 239..253
NoneNo IPR availablePANTHERPTHR23012:SF174E3 UBIQUITIN-PROTEIN LIGASE MARCH-RELATEDcoord: 1..255
NoneNo IPR availableCDDcd16495RING_CH-C4HC3_MARCHcoord: 60..106
e-value: 2.44027E-18
score: 74.6712
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 57..109
IPR033275E3 ubiquitin-protein ligase MARCH-likePANTHERPTHR23012RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAININGcoord: 1..255

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G008610.1CmaCh14G008610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding