CmaCh14G000770 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G000770
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	AdoMet-dependent rRNA methyltransferase spb1
Location	Cma_Chr14: 364973 .. 370011 (+)
RNA-Seq Expression	CmaCh14G000770
Synteny	CmaCh14G000770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTGATCAAGATTTCATTTATCTCTACGCTATCCATGTAAACGTTAACCGTAGGATGTGGTACTCTGAGGTGAAGATTGTATAGCTAGTTCTTATGAGTTGGTTATGAGCACACTGCATAGTGTGTTCTAATCTGGAATTTTTACGCGTTGTTTGATAACCATTTTGTTTTAGTTTTTTATTTTTTGAAAATTGTTCTGTTTTCTCACAATTTCGTTACAATGGTCTTCATCATCTGTTGTAAGGTGGCGTTTGAATTCTCAACCAAATTCCTAAAGTAAAAACAAGTTTTTATTTATTACTATTTTTTATTTTTATTTTTGTTTATAGCTTTCAAAACTTGACATGAATTTTGAAAAAATTGTTAGAAAGTAGATAAACTCATGAGTGGAAGCAATTCTTATAAGGTTAATTTTCAAAATTTAGAAACAAAAAACCCCATGGTTATCAATTGGAGCCTTATCTGTTAATTAGATGTTTTTGCTCCATTTTGATTTTAACCACGAATGTGGCAAATATTTCTGGAAAGAAGGAATTTGACTCTCCAATTGAATTGTTGTTGCAAGGCTTGTTGGCTCTAGACCAAAATTTGTTTATTTTTTTGGGTTGAGGATTCAATACTTTATTTAATATTAAATACACTTAAGACTTCCTTTGATGACCCACTTTAACATTCTTGCTGGGTTTGAATGTTACCAGGAGGCCTGTCTTCTCAACACTACCAGAAACTCTTGCTTGATTCTTCAATCATGGGCAAAGCTTCGAGAGATAAGAGGGTTTAAGCACTATTGCCTTTGACTATTTGTCTTTCTAATTCATTATCTATTTCCATGTTTTATTTACTGTGTCTCATTTAGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGACGACGAATTTAACATATTGGAAGGTTTGTTCTTGTATTGGGCTCCTCTCTATTGAAATATTCTTACATTTCTGCTGGGATGTCAATCACTGAGATGGTCGACATGAATGAGAATAATTATTGCTGCAACATTTTGGATTCCATAACAGATTATTTTCTAGAATCCTCCTGAAATCTGTGTGATTTATTTTCCTTTGTAATCATCCTGTTTTATCATGTACAAGTCATTTGAAACGCTTCTAAGTTAATCATCTACCATGAAATTTTAAATGCAGGAGTCAAGAGAGTGGTGGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTAATTTCTTGGGCGTTTTCTTGTTGCAATTTGTTTATCAAACTAGTGGTCAATGCTTTTTGGGGATTTTTTTTTTCTTCATATAAAACAACTAAATCTAACAAGAATTCTTCAAGTGATAGGCATTGTAAGCATTTTTTGAAAAACATGCCTGCATATGGGGTGCAATACTCTCCTCCCTTCATTGACCCTACATTTTGGTACACTTTTCAAAAGTAATGATAACATTAAGTATTCTTGAAAAAGGGAAGAAAACAACTCGAATTCCTTAATTTTTAAGTACTCTTATAAAAGCTTGTTTTTAAACTTTCATTATCTTATTTTTCAGAACAATATATATTTTTATCTGTATTTTTATATTAACACTCATAGGAGTATTATGCTTCAGTACATTTCACGTTATGAAAAACACTAAATATAAGGAGGTTGATAAAAGCAATGTAGTATGTGTTGCTGCAACTATTGAACAAAAATATAATTAATTTTGGTTAATTATTTCAAACTTGTCTTTACATTTTCTCGCCTACTAATCATATGTAGGTCCTCAGTAGGAAATTGTATCTCCCAGCAAAGCTTTCTCCTGATTCGAAGTAAGTTATGGTAGATCATTTCATGAAAATATAAATTTTTCCTTCCCATCCTTCTCTCCGTTTCCCCCTTGTTTTCTCCCTTTTCATTGATTCTCTAACTTAACTTGCAGGGATGGCGATCATCCTCTTATTGTTGCTATTGATTTACAACCGATGGCTCCAATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTAAGCCGGGTGTACCATGTACGAAGTATTTTTTAACATTTGAAGTGGTAAACGAAGACATATCCAGATCCTAATGCACCGACATGGTCAATTTTGTTTTAAAATTTCTATTTGTGATAATTTTCCTATGTTTTTCTCCATACTGGATTTTTTTCTCTTTGAATGAATACTCACGTTGTGTTAGAAATCTTTTATTTTATTGAACAATTTGATCACATACTAAATTGTCATATGTTTTTCTGCATGGCTTATCAAATATTTAATTTTACCAGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGATGGTGCACCAGATGGTAGATACTTGTGTTTTTTGAATTCTCTGTTGAGGTTCCACCTGATGATATTTTGTTACTGATTATGTGCTTGTTTGTAGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTCCCAGCTCATACTAGCCGTAAGTGCTGCGTTTTCTACAGAGACTGTTTAATTTCTCGAGAAAGTGCTGATGAATAGTTTAAGATATTTTAAAAATGCATGGTATATATGCACATTGACAGTAATGATGTTGCTTCATCTCACATTTACTGTGTGGAAAGGATCAAAAACTAAAACCAACAAGAGAAGCGCTAATGAGTGTCAAATTGTTTGAACCTACAAATAGAAAATAATGTGTAACTAGCGAGCAAAGTAGTTGAGATCCTGTAGGTAAATGGTGGTTGCTGTATTAGGAGGTTATACTGAAGCATTCTAAGGGTTTTTCTATTGTATGTTGCCTGCACTCTTTGATCTTTTTCCTCTATGGACTTAGTTTTGTTGCTTCCATCACTGTCCTCTGCTCTGCCTCGTTTTTCCTCCCTCTCTCTCTTCCTTGAGTTAGTTCTCAATATAGTTGGAGATACATAAGGTTTAAGTAACTGAGCTCATCTCGTTTTTTCCCCCTTTTCTTTGCAGGGTTTAACAATAGTTACTCACGTTCTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACAAGCCTTTTGTATTGTCAGGTATATATTAAATGATTGTTATACAATACCCAATTAAAAAAGCATGTAGCATACTCGAGTAACGAAGAAATTTATGGTGTTCTCACTAATGGCATCTACAATATGAATCTCTCTTCTCTTCTACATTTTTTTTCCAGTCAAGTTGGAAAATTTCCTGTGAGCTCTTCTGTTCCTTATAATTAATGCATTGACGTATAGAATTCTTTTAGATGCGTTTAGCACTAGTAAAACTTGTCAAGTATTATCATGGATTAAATAAGGATCTTAATCAAAGATTGGTGTTTGTTGTTTCATACATGAAGTGATTGTGCCGTCAATGATGTTTTACATTTTTGGGTTTTTCTGTTGCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCCAGTATAGGTAACGTTTTGGTTTTCCCACTTTTTTTTGTCCATTGGTAGCTATAGAGGTCTATCTTTCGGTTATGATTGAACTTGTTTTCAGAAAATGATTTTCTCCTAATTGAACATTGCCTTTTTCTCTTTATAGCGTCTGGTTCATAAGATTCTTGCGCATGAATTTTTAGATACCCATCGTACTTGGATTCCGTAAATCAACTTTAGATTGTTATCGTTTAATTCTAGGTGGTGGGACTGGTGTCCACCCAACAGCAAATTGTGGAGTTAGCTCATTTTCGTGGTGACATCTGTAATATAAAAAAGATGTAATAGAGGACAAAATAGTCCAGGGCAAAAGTAAAATTTTTCTAAACTTTCATGGTTGTGTTCGTGAACTAGAAAAAGTTCCAATTACATCCAGCAAGCGTTCTTTTATCACTTGCTTAAAATTTTAAATTATCAGCAAGTGATTATTTGGCGTTATGTACCAGAAGTAGGCATGTTAAACAAAATTATGGAAGATGCGAAAAGACGCGATGGGAGCAATTACATTTTAAACTGAATTTCTGGGTTTGTCTGCCCTTAGCTTAGCTAGTTGGCTTTCTCTTTTATATCACATGTTGGTATATCATTTCAAATCATTATTCGTTAGTCTAATCAACGTTCCATGAAAACATTTAAGCCACAATGTTAACCATGGGAAATTAATGGGATCAAAATCAAGAGTTCAAGAACATAATTGGAGCATTTAAAAGTTGAATGGATTAAGTGGATACATCTTGAACTAAATCTGCGATTCATTCCATTGATTTCTTTTAGTCACCTAGGTGAACCCACGGTAAAGACTTTTAAACAAAGTTCTGATTATGATTTAACGTTATGTTCATGTCGAAGCTAATATGTGGATTGGATATGAATGCAGAGGCATTTGCAGTCTGTGAAAACTATTCTCCTCTCGAAGGTTTCAACCCAAAAGATCTACATCGCCTGCTGGAAAAGGTGGGAAGTCCATCAGGAGGAGATGACTTAGGTAAACTAATGTACTGCCTTGATTGAGTGTTTACGTATCGATGGATGGACGCATCCTAAGATCAAACATGTTCTTGTCCTGTTTTTCAAGATTTATTTGTTTTACAGCTGGTGCTATGACCGAGCTGTCTTCCTGTCTTTAGGCATTTTAAAATTAGTGTCCTTGCTGATTTTGATGTGGTCAATTGCCTGGTATTTTTCGTTACACAAGTGGAAGTAATAGCATTGCTATTTTGCTACATTGTGTCCCTCGCAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCATGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAACCTCCTATCGCCCCACCTTACAAGAGGGCTCTTGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTCTCCTCGGATTCCTGA

mRNA sequence

ATGCCTGATCAAGATTTCATTTATCTCTACGCTATCCATGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGACGACGAATTTAACATATTGGAAGGAGTCAAGAGAGTGGTGGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTCAGTAGGAAATTGTATCTCCCAGCAAAGCTTTCTCCTGATTCGAAGGATGGCGATCATCCTCTTATTGTTGCTATTGATTTACAACCGATGGCTCCAATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGATGGTGCACCAGATGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTCCCAGCTCATACTAGCCGGTTTAACAATAGTTACTCACGTTCTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACAAGCCTTTTGTATTGTCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCCAGTATAGAGGCATTTGCAGTCTGTGAAAACTATTCTCCTCTCGAAGGTTTCAACCCAAAAGATCTACATCGCCTGCTGGAAAAGGTGGGAAGTCCATCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCATGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAACCTCCTATCGCCCCACCTTACAAGAGGGCTCTTGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTCTCCTCGGATTCCTGA

Coding sequence (CDS)

ATGCCTGATCAAGATTTCATTTATCTCTACGCTATCCATGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGACGACGAATTTAACATATTGGAAGGAGTCAAGAGAGTGGTGGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTCAGTAGGAAATTGTATCTCCCAGCAAAGCTTTCTCCTGATTCGAAGGATGGCGATCATCCTCTTATTGTTGCTATTGATTTACAACCGATGGCTCCAATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGATGGTGCACCAGATGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTCCCAGCTCATACTAGCCGGTTTAACAATAGTTACTCACGTTCTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACAAGCCTTTTGTATTGTCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCCAGTATAGAGGCATTTGCAGTCTGTGAAAACTATTCTCCTCTCGAAGGTTTCAACCCAAAAGATCTACATCGCCTGCTGGAAAAGGTGGGAAGTCCATCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCATGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGAGGGAACTTATCGGAGCTTAGATCCTGTGCAACCTCCTATCGCCCCACCTTACAAGAGGGCTCTTGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTCTCCTCGGATTCCTGA

Protein sequence

MPDQDFIYLYAIHDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIRELEKLSSDS

Homology

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BLAST of CmaCh14G000770 vs. ExPASy Swiss-Prot
Match: Q9UET6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapiens OX=9606 GN=FTSJ1 PE=1 SV=2)

HSP 1 Score: 344.0 bits (881), Expect = 1.9e-93
Identity = 168/291 (57.73%), Postives = 207/291 (71.13%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           D+YYR AKE GWRARSAFKLLQ+D EF + +GV R VDLCAAPGSWSQVLS+K+      
Sbjct: 10  DVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLSQKI------ 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
               +   H  +VA+DLQ MAP+ GV+Q+QGDIT   TA+ +I+HF GC ADLVVCDGAP
Sbjct: 70  --GGQGSGH--VVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPADLVVCDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHD+DE++Q+QL+LA L I THVL+ GG F+AKIFRG+D +LLY QL++FF  V  
Sbjct: 130 DVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLYSQLQVFFSSVLC 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKP+SSRNSSIEAFAVC+ Y P EGF P     LL+    P            L+GP ++
Sbjct: 190 AKPRSSRNSSIEAFAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQ--------LDGPTRI 249

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 305
            +PF+ CGDLS YDSDRSYPL       Y+   P QPPI+PPY+ A  +K+
Sbjct: 250 IVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKR 282

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BLAST of CmaCh14G000770 vs. ExPASy Swiss-Prot
Match: Q22031 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=R74.7 PE=3 SV=3)

HSP 1 Score: 314.7 bits (805), Expect = 1.2e-84
Identity = 166/303 (54.79%), Postives = 206/303 (67.99%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYR AKE  WRARSAFKL+QIDDEF IL+GV+R VDLCAAPGSWSQVLS++LY     
Sbjct: 10  DIYYRLAKENKWRARSAFKLMQIDDEFQILKGVRRAVDLCAAPGSWSQVLSKRLY----- 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
               ++     IVAIDLQPMAPI GVIQ+QGDIT+  TA  VI+HF G K+D+V+CDGAP
Sbjct: 70  ----EEDQEAKIVAIDLQPMAPIPGVIQLQGDITSVDTANQVIKHFSGEKSDIVICDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTG+H +DEF+Q++LILA   I +HVL+EGG F+AKIFR +++SLLY Q+K +F  V  
Sbjct: 130 DVTGIHSLDEFMQAELILAAFNITSHVLKEGGNFLAKIFRSRNSSLLYAQMKKYFKKVYL 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKP+SSR SS EAF +C +YSP EGF P              G   LD +      P+ +
Sbjct: 190 AKPRSSRQSSCEAFVLCLDYSPPEGFVP------------TMGKTSLDATDASAISPD-I 249

Query: 254 YIPFLACGDLSGYDSDRSYPL------PRG-----AEGTYRSLDPVQPPIAPPYKRALEM 306
              F+ CGDLSG+DS++SYPL      P+G      +  Y   D VQPP  P YK AL+ 
Sbjct: 250 IDGFVTCGDLSGWDSEKSYPLDIDACFPKGEIDEEQKKRYEFKDVVQPPTDPAYKAALDK 290

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BLAST of CmaCh14G000770 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.0e-83
Identity = 159/292 (54.45%), Postives = 207/292 (70.89%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLY--LPA 73
           D YYR AKE+GWRARSAFKLLQ++++FN+ EG KRVVDLCAAPGSWSQVLSR+L   +  
Sbjct: 10  DAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWSQVLSRELLKNIDT 69

Query: 74  KLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDG 133
            ++ D K    P+IVA+DLQPMAPI+GV  +Q DIT+  T  +++ HF    ADLVV DG
Sbjct: 70  SIAADEK----PMIVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNEPADLVVSDG 129

Query: 134 APDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVV 193
           APDVTGLHD+DE++Q+Q++LA   +   VL+ GGKF+AKIFRG+D SLLY QL+L F  V
Sbjct: 130 APDVTGLHDLDEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLYSQLRLMFRKV 189

Query: 194 TFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPN 253
           + AKP+SSR SSIE+F VCE+++P   F P DL + L  V  P+   ++           
Sbjct: 190 SCAKPRSSRASSIESFVVCEDFNPPSNFQP-DLTKPL-CVIDPTNAHEI----------- 249

Query: 254 KVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMK 304
               PF+ACGDL GYD+D +YP+    +    +LD +QPP APPYKRA+E+K
Sbjct: 250 ---APFIACGDLDGYDADATYPVEINMKKA--TLDVIQPPTAPPYKRAIELK 279

[Close]

BLAST of CmaCh14G000770 vs. ExPASy Swiss-Prot
Match: Q54VA8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjA PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 5.4e-77
Identity = 147/265 (55.47%), Postives = 190/265 (71.70%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKL+QID+E+ I EGVKRVVDLCAAPGSWSQVLSR++Y   K 
Sbjct: 10  DIYYRKAKEEGWRARSAFKLIQIDEEYQIFEGVKRVVDLCAAPGSWSQVLSRRIYGDGK- 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
            PD K      IVA+DLQ MAP++GV+Q++GDIT   T++ +I HFDG  ADL++ DGAP
Sbjct: 70  DPDVK------IVAVDLQEMAPLKGVVQIKGDITKYETSKQIISHFDGSLADLIISDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHD+D + QSQLIL+ L I TH L+ GG F+AK+F+G D SL+Y Q+KLFF  V+F
Sbjct: 130 DVTGLHDIDFYGQSQLILSALNITTHTLKIGGTFVAKMFKGDDMSLMYSQMKLFFEHVSF 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLE---KVGSPSGGDDLDCSSGWLEGP 253
            KP SSR SS+E F +C NY P   +NPK +   LE   K+ + +  +  + ++  ++  
Sbjct: 190 VKPSSSRESSLENFILCRNYQPPLNYNPKIIDPALENHNKILNNNNNNSNNNNNCNIDNE 249

Query: 254 N--------KVYIPFLACGDLS-GY 267
           N         + +PF+ACGDL+ GY
Sbjct: 250 NDNEFLKTDSLIVPFIACGDLNLGY 267

[Close]

BLAST of CmaCh14G000770 vs. ExPASy Swiss-Prot
Match: P38238 (tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM7 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.9e-75
Identity = 152/299 (50.84%), Postives = 203/299 (67.89%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILE--GVKRVVDLCAAPGSWSQVLSRKLYLPA 73
           D+YYRKAKE+G+RARSAFKLLQ++D+F+ L+   +KRVVDLCAAPGSWSQVLSRKL+   
Sbjct: 10  DLYYRKAKEQGYRARSAFKLLQLNDQFHFLDDPNLKRVVDLCAAPGSWSQVLSRKLF--- 69

Query: 74  KLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDG 133
             SP S D +   IV++DLQPM+PI  V  +Q DIT+ +T   +++ F   KAD V  DG
Sbjct: 70  DESP-SSDKEDRKIVSVDLQPMSPIPHVTTLQADITHPKTLARILKLFGNEKADFVCSDG 129

Query: 134 APDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVV 193
           APDVTGLHD+DE+VQ QLI++ L +   +L++GG F+AKIFRG+D  +LY QL   F  +
Sbjct: 130 APDVTGLHDLDEYVQQQLIMSALQLTACILKKGGTFVAKIFRGRDIDMLYSQLGYLFDKI 189

Query: 194 TFAKPKSSRNSSIEAFAVCENYSPLEGFNPK-DLHRLLEKVGSPSGGDDL---DCSSGWL 253
             AKP+SSR +S+EAF VC  Y+P   + PK D++  +++       + L   D  S W 
Sbjct: 190 VCAKPRSSRGTSLEAFIVCLGYNPPSNWTPKLDVNTSVDEFFQGCFLNKLCISDKLSHWN 249

Query: 254 EGPNKVYIPFLACGDLSGYDSDRSY-PLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKA 306
           E    +   F+ACG L  +DSD +Y  LP    GT  SLDPVQ P  PPYK+ALE+K++
Sbjct: 250 EEERNI-AEFMACGSLQSFDSDATYHDLPSSVAGTSSSLDPVQSPTNPPYKKALELKRS 303

[Close]

BLAST of CmaCh14G000770 vs. ExPASy TrEMBL
Match: A0A6J1IYY9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481931 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 1.8e-176
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
           SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV
Sbjct: 190 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 249

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 313
           YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL
Sbjct: 250 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 309

Query: 314 EKLSSDS 321
           EKLSSDS
Sbjct: 310 EKLSSDS 316

[Close]

BLAST of CmaCh14G000770 vs. ExPASy TrEMBL
Match: A0A6J1F1P7 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441398 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.4e-175
Identity = 305/307 (99.35%), Postives = 305/307 (99.35%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKLLQIDDEFNI EGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
           SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV
Sbjct: 190 AKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 249

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 313
           YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL
Sbjct: 250 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 309

Query: 314 EKLSSDS 321
           EKLSSDS
Sbjct: 310 EKLSSDS 316

[Close]

BLAST of CmaCh14G000770 vs. ExPASy TrEMBL
Match: A0A5A7TQN9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00950 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.3e-170
Identity = 298/310 (96.13%), Postives = 302/310 (97.42%), Query Frame = 0


Query: 11  AIHDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLP 70
           A+ DIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLSRKLYLP
Sbjct: 67  ALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLP 126

Query: 71  AKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCD 130
           AKLSPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCD
Sbjct: 127 AKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCD 186

Query: 131 GAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPV 190
           GAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QLKLFFPV
Sbjct: 187 GAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPV 246

Query: 191 VTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 250
           VTFAKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 247 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 306

Query: 251 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 310
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 307 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 366

Query: 311 RELEKLSSDS 321
           RELEKLS DS
Sbjct: 367 RELEKLSLDS 376

[Close]

BLAST of CmaCh14G000770 vs. ExPASy TrEMBL
Match: A0A1S3B6C8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 9.7e-170
Identity = 297/307 (96.74%), Postives = 300/307 (97.72%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
           SPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QLKLFFPVVTF
Sbjct: 130 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPVVTF 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV
Sbjct: 190 AKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 249

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 313
           YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL
Sbjct: 250 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 309

Query: 314 EKLSSDS 321
           EKLS DS
Sbjct: 310 EKLSLDS 316

[Close]

BLAST of CmaCh14G000770 vs. ExPASy TrEMBL
Match: A0A0A0LCV6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G732420 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.4e-168
Identity = 295/307 (96.09%), Postives = 299/307 (97.39%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
           SPD KDG+ PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QLKLFFPVVTF
Sbjct: 130 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPVVTF 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKPKSSRNSSIEAFAVCENYSP EGFN KDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV
Sbjct: 190 AKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 249

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 313
           YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL
Sbjct: 250 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 309

Query: 314 EKLSSDS 321
           EKLS DS
Sbjct: 310 EKLSLDS 316

[Close]

BLAST of CmaCh14G000770 vs. NCBI nr
Match: XP_022983316.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita maxima] >XP_022983317.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita maxima])

HSP 1 Score: 628.2 bits (1619), Expect = 3.8e-176
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
           SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV
Sbjct: 190 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 249

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 313
           YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL
Sbjct: 250 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 309

Query: 314 EKLSSDS 321
           EKLSSDS
Sbjct: 310 EKLSSDS 316

[Close]

BLAST of CmaCh14G000770 vs. NCBI nr
Match: KAG6580486.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 624.0 bits (1608), Expect = 7.1e-175
Identity = 305/307 (99.35%), Postives = 305/307 (99.35%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKLLQIDDEFNI EGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 30  DIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 89

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
           SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 90  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 149

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF
Sbjct: 150 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 209

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV
Sbjct: 210 AKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 269

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 313
           YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL
Sbjct: 270 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 329

Query: 314 EKLSSDS 321
           EKLSSDS
Sbjct: 330 EKLSSDS 336

[Close]

BLAST of CmaCh14G000770 vs. NCBI nr
Match: XP_022934133.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_022934134.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_023528668.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >XP_023528669.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >KAG7017237.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 624.0 bits (1608), Expect = 7.1e-175
Identity = 305/307 (99.35%), Postives = 305/307 (99.35%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKLLQIDDEFNI EGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
           SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV
Sbjct: 190 AKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 249

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 313
           YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL
Sbjct: 250 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 309

Query: 314 EKLSSDS 321
           EKLSSDS
Sbjct: 310 EKLSSDS 316

[Close]

BLAST of CmaCh14G000770 vs. NCBI nr
Match: XP_038903231.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 610.5 bits (1573), Expect = 8.1e-171
Identity = 299/307 (97.39%), Postives = 302/307 (98.37%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKLLQID+EFNI EGV+RVVDLCAAPGSWSQVLSRKL+LPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVERVVDLCAAPGSWSQVLSRKLHLPAKL 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
           SPDSKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV
Sbjct: 190 AKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 249

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIREL 313
           YIPFLACGDLSGYDSDRSYPLPRGAEGTYR LDPVQPPIAPPYKRALEMKKASSQGIREL
Sbjct: 250 YIPFLACGDLSGYDSDRSYPLPRGAEGTYRCLDPVQPPIAPPYKRALEMKKASSQGIREL 309

Query: 314 EKLSSDS 321
           EKLS DS
Sbjct: 310 EKLSLDS 316

[Close]

BLAST of CmaCh14G000770 vs. NCBI nr
Match: KAA0044157.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo var. makuwa] >TYK24978.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 607.4 bits (1565), Expect = 6.9e-170
Identity = 298/310 (96.13%), Postives = 302/310 (97.42%), Query Frame = 0


Query: 11  AIHDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLP 70
           A+ DIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLSRKLYLP
Sbjct: 67  ALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLP 126

Query: 71  AKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCD 130
           AKLSPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCD
Sbjct: 127 AKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCD 186

Query: 131 GAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPV 190
           GAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QLKLFFPV
Sbjct: 187 GAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQLKLFFPV 246

Query: 191 VTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 250
           VTFAKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 247 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 306

Query: 251 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 310
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 307 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 366

Query: 311 RELEKLSSDS 321
           RELEKLS DS
Sbjct: 367 RELEKLSLDS 376

[Close]

BLAST of CmaCh14G000770 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 566.6 bits (1459), Expect = 1.3e-161
Identity = 274/296 (92.57%), Postives = 287/296 (96.96%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           DIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLSR+LYLPAK 
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRQLYLPAKS 69

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 133
           S +SKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 134 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTF 193
           DVTGLHDMDEFVQSQLILAGLTIVTH+L+EGGKFIAKIFRGKDTSLLYCQLKLFFP VTF
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAKIFRGKDTSLLYCQLKLFFPTVTF 189

Query: 194 AKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKV 253
           AKPKSSRNSSIEAFAVCENYSP EGFNP+DLHRLLEKVGSPSGG DLDCSSGWLEGPNKV
Sbjct: 190 AKPKSSRNSSIEAFAVCENYSPPEGFNPRDLHRLLEKVGSPSGGSDLDCSSGWLEGPNKV 249

Query: 254 YIPFLACGDLSGYDSDRSYPLPRGAEG-TYRSLDPVQPPIAPPYKRALEMKKASSQ 309
           YIPFLACGDL+GYDSDRSYPLPR A+G +Y+SLDP+QPPIAPPYKRALE+KKAS+Q
Sbjct: 250 YIPFLACGDLTGYDSDRSYPLPREADGSSYQSLDPIQPPIAPPYKRALELKKASAQ 305

[Close]

BLAST of CmaCh14G000770 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.8e-30
Identity = 78/230 (33.91%), Postives = 127/230 (55.22%), Query Frame = 0


Query: 14  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 73
           D YYR AKE G+R+R+++KLLQ+D ++++L     V+DLCAAPG W QV   K+ + +  
Sbjct: 11  DKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVAVEKVPVGS-- 70

Query: 74  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDIT----NARTAEVVIRHFDGCKA-DLVV 133
                     L++ IDL P+ P+ G + +  DIT     ++  +V+ +H  G  A +LV+
Sbjct: 71  ----------LVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQH--GVSAFNLVL 130

Query: 134 CDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKD-TSLLYCQLKLF 193
            DG+P+V G    +   Q+ L++  + + T  L   G  + K+FR +D  S+LYC  +LF
Sbjct: 131 HDGSPNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTKVFRSRDYNSVLYCLGRLF 190

Query: 194 FPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPK--DLHRLLEKVGSPS 236
             V  F KP +SR++S E + V   Y      +P+  D   L ++   P+
Sbjct: 191 EKVEVF-KPPASRSASAETYLVGLKYLAPAKIDPRLLDYRHLFKESAEPT 225

[Close]

BLAST of CmaCh14G000770 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 1.5e-21
Identity = 46/48 (95.83%), Postives = 47/48 (97.92%), Query Frame = 0


Query: 14 DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQ 62
          DIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQ
Sbjct: 10 DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57

[Close]

BLAST of CmaCh14G000770 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 75.5 bits (184), Expect = 8.8e-14
Identity = 55/227 (24.23%), Postives = 99/227 (43.61%), Query Frame = 0


Query: 12  IHDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPA 71
           + D +YR+A+  G+ ARSAFKLLQI  ++ +++    V+DL  APG+W QV  +      
Sbjct: 6   VPDFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQ------ 65

Query: 72  KLSPDSKDGDHPLIVAIDLQ----PMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLV 131
            L P    G   ++V +D++    P      V  +  D+ N    ++           ++
Sbjct: 66  SLGPLRSGG---IVVGMDIKKVKVPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSVI 125

Query: 132 VCDGAPDVTGLHDMDEFVQSQLILAGLTIVT---------------------HVLREGGK 191
           + D    V+G+   D  + ++L +  L +                        VLR GG 
Sbjct: 126 LSDMCHSVSGITTRDAALSAELGMRALDLAVGQAAISQSPNDDDGGPNESRPGVLRHGGH 185

Query: 192 FIAKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENY 214
            + K+   +D        K  F   ++ +PK++R SS E + +C+ +
Sbjct: 186 LVIKLLESEDAQDFARICKPIFNKASWLRPKATRPSSREIYLICQGF 223

The following BLAST results are available for this feature:

BLAST of CmaCh14G000770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UET6	1.9e-93	57.73	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapien...	[more]
Q22031	1.2e-84	54.79	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabdi...	[more]
O36015	3.0e-83	54.45	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch...	[more]
Q54VA8	5.4e-77	55.47	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyosteli...	[more]
P38238	3.9e-75	50.84	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevi...	[more]

BLAST of CmaCh14G000770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IYY9	1.8e-176	100.00	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]
A0A6J1F1P7	3.4e-175	99.35	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]
A0A5A7TQN9	3.3e-170	96.13	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A1S3B6C8	9.7e-170	96.74	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A0A0LCV6	2.4e-168	96.09	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sat...	[more]

BLAST of CmaCh14G000770 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022983316.1	3.8e-176	100.00	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita max...	[more]
KAG6580486.1	7.1e-175	99.35	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucu...	[more]
XP_022934133.1	7.1e-175	99.35	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita mos...	[more]
XP_038903231.1	8.1e-171	97.39	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa his...	[more]
KAA0044157.1	6.9e-170	96.13	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cu...	[more]

BLAST of CmaCh14G000770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01230.1	1.3e-161	92.57	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G25730.1	1.8e-30	33.91	FtsJ-like methyltransferase family protein	[more]
AT5G01230.2	1.5e-21	95.83	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G13830.1	8.8e-14	24.23	FtsJ-like methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002877	Ribosomal RNA methyltransferase FtsJ domain	PFAM	PF01728	FtsJ	coord: 25..213 e-value: 4.8E-64 score: 215.7
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 24..218 e-value: 3.4E-73 score: 247.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 304..320
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 301..320
None	No IPR available	PANTHER	PTHR10920	RIBOSOMAL RNA METHYLTRANSFERASE	coord: 13..312
None	No IPR available	PANTHER	PTHR10920:SF25	TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED	coord: 13..312
IPR028590	tRNA (cytidine(32)/guanosine(34)-2-O)-methyltransferase, Trm7	HAMAP	MF_03162	RNA_methyltr_E_TRM7	coord: 5..272 score: 30.921141
IPR015507	Ribosomal RNA large subunit methyltransferase E	HAMAP	MF_01547	RNA_methyltr_E	coord: 9..214 score: 31.362827
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 24..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G000770.1	CmaCh14G000770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002181	cytoplasmic translation
biological_process	GO:0002128	tRNA nucleoside ribose methylation
biological_process	GO:0032259	methylation
biological_process	GO:0001510	RNA methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008175	tRNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity