CmaCh13G010350 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh13G010350
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protein ASPARTIC PROTEASE IN GUARD CELL 1-like
Location	Cma_Chr13: 7908238 .. 7909725 (-)
RNA-Seq Expression	CmaCh13G010350
Synteny	CmaCh13G010350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACTGCAAATCTGCAACCTCTGTTCTCCTTCGCCATCTTCTTTTTCTCTCTCTTGCCCTTCACTCTGTGTCGCACTTCGCCGGAGCTTTCTCACTCCTCCTCCTCCTCCTCCGCTTTTCTCGATGTTTCGGCTTCACTGAAACAAGCCAACCAGGTCCTGAAATTCGACCCCAAGGCTTTGCTATCCTTCCAGCAGCAGAATGAGGTTGTTCCGGCGAACTCTTCTTCCTCCTTTTCTCTGCAACTGCACTCTCGCGACGCTCTTCAAAATGCCGGACATAAGGACTACAAGAGCCTGGTCCTTTCCCGGCTGGACCGGGACTCTTCTAGAGTTAAATCGCTCAACGATAGGCTTGAATTTTCCCTCAGTGACTTGAAGAGGTCGGATCTTCAGCCGTTGCAAACGGAGATTCTGCCAGAGGATCTGAGCACTCCGATTGTCTCTGGCACCAGTCAAGGAAGCGGCGAGTATTTCTCCCGCATCGGCGTCGGACAACCGGCTAAGCCGTTCTATATGGTTCTTGACACCGGCAGCGATATCAATTGGCTTCAATGTCAGCCTTGTACGGACTGTTACCAGCAAACCGACCCGATTTTTGACCCGAGAACTTCTTCTTCTTTTGCTTCACTTGCCTGCGAATCTCCGCAGTGTCAAGCTCTTGAAACGTCTGGCTGTCGTGCTGCGAAGTGTCTTTACCAGGTCGCGTACGGCGATGGCTCCTTCACTGTCGGTGAGTTCGTTACTGAATCTCTGTCGTTTGGTAACTCCGGGACGATTAACAATGTCGCTCTTGGCTGCGGCCACGACAACGAAGGATTATTCGTCGGATCCGCAGGATTACTCGGACTCGGCGGCGGATCACTATCCCTCATTTCTCAGATGAAAGCGTCGTCGTTCTCATACTGTCTTGTGGACCGCGACTCTAGTTCGTCTTCAGCTCTCGAGTTCAATTCCGCTGCACCGAGTGACACAGTGAATGCTCCATTACTCAGAAGCGGAAGAGTCGATACATTCTACTACGTCGGACTCGTCGGATTGAGCGTCGGCGGCCAGTTACTGTCAATTCCTCCTTCTCTGTTTCAAATGGACGATGCCGGCTACGGCGGTATCATCGTCGATTCAGGAACCGCCATAACTCGTCTGCAGACTCAAGCCTACAACACCCTTCGCGACGCCTTCGTGAGTCGCACACCATACCTGAGGAGGACAAACGGCGTCGCATTATTCGACACATGTTACGATCTGTCGTCGCAGTCGAGAGTCACCATTCCAACGCTGTCATTTCAATTCGCTGGCGGGAAGTCTCTGCAGCTGCCGCCAAAGAACTACCTCATCCCGGTGGACTCCGTCGGAACTTTCTGTTTGGCGTTCGCCCCGACGACGTCGTCTTTGTCCATCATAGGAAACGTCCAGCAGCAAGGGACACGTGTCCACTTCGATTTGGCAAATTCACTCTTAGGGTTTTCACCCAACAAATGTTAG

mRNA sequence

ATGGCGACTGCAAATCTGCAACCTCTGTTCTCCTTCGCCATCTTCTTTTTCTCTCTCTTGCCCTTCACTCTGTGTCGCACTTCGCCGGAGCTTTCTCACTCCTCCTCCTCCTCCTCCGCTTTTCTCGATGTTTCGGCTTCACTGAAACAAGCCAACCAGGTCCTGAAATTCGACCCCAAGGCTTTGCTATCCTTCCAGCAGCAGAATGAGGTTGTTCCGGCGAACTCTTCTTCCTCCTTTTCTCTGCAACTGCACTCTCGCGACGCTCTTCAAAATGCCGGACATAAGGACTACAAGAGCCTGGTCCTTTCCCGGCTGGACCGGGACTCTTCTAGAGTTAAATCGCTCAACGATAGGCTTGAATTTTCCCTCAGTGACTTGAAGAGGTCGGATCTTCAGCCGTTGCAAACGGAGATTCTGCCAGAGGATCTGAGCACTCCGATTGTCTCTGGCACCAGTCAAGGAAGCGGCGAGTATTTCTCCCGCATCGGCGTCGGACAACCGGCTAAGCCGTTCTATATGGTTCTTGACACCGGCAGCGATATCAATTGGCTTCAATGTCAGCCTTGTACGGACTGTTACCAGCAAACCGACCCGATTTTTGACCCGAGAACTTCTTCTTCTTTTGCTTCACTTGCCTGCGAATCTCCGCAGTGTCAAGCTCTTGAAACGTCTGGCTGTCGTGCTGCGAAGTGTCTTTACCAGGTCGCGTACGGCGATGGCTCCTTCACTGTCGGTGAGTTCGTTACTGAATCTCTGTCGTTTGGTAACTCCGGGACGATTAACAATGTCGCTCTTGGCTGCGGCCACGACAACGAAGGATTATTCGTCGGATCCGCAGGATTACTCGGACTCGGCGGCGGATCACTATCCCTCATTTCTCAGATGAAAGCGTCGTCGTTCTCATACTGTCTTGTGGACCGCGACTCTAGTTCGTCTTCAGCTCTCGAGTTCAATTCCGCTGCACCGAGTGACACAGTGAATGCTCCATTACTCAGAAGCGGAAGAGTCGATACATTCTACTACGTCGGACTCGTCGGATTGAGCGTCGGCGGCCAGTTACTGTCAATTCCTCCTTCTCTGTTTCAAATGGACGATGCCGGCTACGGCGGTATCATCGTCGATTCAGGAACCGCCATAACTCGTCTGCAGACTCAAGCCTACAACACCCTTCGCGACGCCTTCGTGAGTCGCACACCATACCTGAGGAGGACAAACGGCGTCGCATTATTCGACACATGTTACGATCTGTCGTCGCAGTCGAGAGTCACCATTCCAACGCTGTCATTTCAATTCGCTGGCGGGAAGTCTCTGCAGCTGCCGCCAAAGAACTACCTCATCCCGGTGGACTCCGTCGGAACTTTCTGTTTGGCGTTCGCCCCGACGACGTCGTCTTTGTCCATCATAGGAAACGTCCAGCAGCAAGGGACACGTGTCCACTTCGATTTGGCAAATTCACTCTTAGGGTTTTCACCCAACAAATGTTAG

Coding sequence (CDS)

ATGGCGACTGCAAATCTGCAACCTCTGTTCTCCTTCGCCATCTTCTTTTTCTCTCTCTTGCCCTTCACTCTGTGTCGCACTTCGCCGGAGCTTTCTCACTCCTCCTCCTCCTCCTCCGCTTTTCTCGATGTTTCGGCTTCACTGAAACAAGCCAACCAGGTCCTGAAATTCGACCCCAAGGCTTTGCTATCCTTCCAGCAGCAGAATGAGGTTGTTCCGGCGAACTCTTCTTCCTCCTTTTCTCTGCAACTGCACTCTCGCGACGCTCTTCAAAATGCCGGACATAAGGACTACAAGAGCCTGGTCCTTTCCCGGCTGGACCGGGACTCTTCTAGAGTTAAATCGCTCAACGATAGGCTTGAATTTTCCCTCAGTGACTTGAAGAGGTCGGATCTTCAGCCGTTGCAAACGGAGATTCTGCCAGAGGATCTGAGCACTCCGATTGTCTCTGGCACCAGTCAAGGAAGCGGCGAGTATTTCTCCCGCATCGGCGTCGGACAACCGGCTAAGCCGTTCTATATGGTTCTTGACACCGGCAGCGATATCAATTGGCTTCAATGTCAGCCTTGTACGGACTGTTACCAGCAAACCGACCCGATTTTTGACCCGAGAACTTCTTCTTCTTTTGCTTCACTTGCCTGCGAATCTCCGCAGTGTCAAGCTCTTGAAACGTCTGGCTGTCGTGCTGCGAAGTGTCTTTACCAGGTCGCGTACGGCGATGGCTCCTTCACTGTCGGTGAGTTCGTTACTGAATCTCTGTCGTTTGGTAACTCCGGGACGATTAACAATGTCGCTCTTGGCTGCGGCCACGACAACGAAGGATTATTCGTCGGATCCGCAGGATTACTCGGACTCGGCGGCGGATCACTATCCCTCATTTCTCAGATGAAAGCGTCGTCGTTCTCATACTGTCTTGTGGACCGCGACTCTAGTTCGTCTTCAGCTCTCGAGTTCAATTCCGCTGCACCGAGTGACACAGTGAATGCTCCATTACTCAGAAGCGGAAGAGTCGATACATTCTACTACGTCGGACTCGTCGGATTGAGCGTCGGCGGCCAGTTACTGTCAATTCCTCCTTCTCTGTTTCAAATGGACGATGCCGGCTACGGCGGTATCATCGTCGATTCAGGAACCGCCATAACTCGTCTGCAGACTCAAGCCTACAACACCCTTCGCGACGCCTTCGTGAGTCGCACACCATACCTGAGGAGGACAAACGGCGTCGCATTATTCGACACATGTTACGATCTGTCGTCGCAGTCGAGAGTCACCATTCCAACGCTGTCATTTCAATTCGCTGGCGGGAAGTCTCTGCAGCTGCCGCCAAAGAACTACCTCATCCCGGTGGACTCCGTCGGAACTTTCTGTTTGGCGTTCGCCCCGACGACGTCGTCTTTGTCCATCATAGGAAACGTCCAGCAGCAAGGGACACGTGTCCACTTCGATTTGGCAAATTCACTCTTAGGGTTTTCACCCAACAAATGTTAG

Protein sequence

MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASSFSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLLGFSPNKC

Homology

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BLAST of CmaCh13G010350 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 4.2e-161
Identity = 291/497 (58.55%), Postives = 371/497 (74.65%), Query Frame = 0


Query: 8   PLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDP--KALLSF 67
           P F   +   +L  F     +   S S+   +  LDV +SL+Q   +L  DP   +L + 
Sbjct: 4   PRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTT 63

Query: 68  QQQNEVVPA--NSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFS 127
           + ++   P   NSSS  SL+LHSRD    + HKDYKSL LSRL+RDSSRV  +  ++ F+
Sbjct: 64  KPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFA 123

Query: 128 LSDLKRSDLQPL---QTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 187
           +  + RSDL+P+    T    EDL+TP+VSG SQGSGEYFSRIGVG PAK  Y+VLDTGS
Sbjct: 124 VEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGS 183

Query: 188 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 247
           D+NW+QC+PC DCYQQ+DP+F+P +SS++ SL C +PQC  LETS CR+ KCLYQV+YGD
Sbjct: 184 DVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGD 243

Query: 248 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 307
           GSFTVGE  T++++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+ +QMKA+S
Sbjct: 244 GSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATS 303

Query: 308 FSYCLVDRDSSSSSALEFNSA-APSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPP 367
           FSYCLVDRDS  SS+L+FNS         APLLR+ ++DTFYYVGL G SVGG+ + +P 
Sbjct: 304 FSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPD 363

Query: 368 SLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRR-TNGVALFDTCYDLS 427
           ++F +D +G GG+I+D GTA+TRLQTQAYN+LRDAF+  T  L++ ++ ++LFDTCYD S
Sbjct: 364 AIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFS 423

Query: 428 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 487
           S S V +PT++F F GGKSL LP KNYLIPVD  GTFC AFAPT+SSLSIIGNVQQQGTR
Sbjct: 424 SLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTR 483

Query: 488 VHFDLANSLLGFSPNKC 496
           + +DL+ +++G S NKC
Sbjct: 484 ITYDLSKNVIGLSGNKC 500

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BLAST of CmaCh13G010350 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 8.3e-109
Identity = 240/495 (48.48%), Postives = 310/495 (62.63%), Query Frame = 0


Query: 9   LFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQ 68
           LFS   FF SL  F+   +   L  +S S      VS            D ++LL  + +
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDS-------DSESLLESEFE 68

Query: 69  NEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLK 128
           +    + SSSS +L L   DAL  + +K    L  SRL RDS RVKS+        +   
Sbjct: 69  SG-SDSESSSSITLNLDHIDAL--SSNKTPDELFSSRLQRDSRRVKSI-------ATLAA 128

Query: 129 RSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQ 188
           +   + +     P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC 
Sbjct: 129 QIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA 188

Query: 189 PCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGC--RAAKCLYQVAYGDGSFTVG 248
           PC  CY Q+DPIFDPR S ++A++ C SP C+ L+++GC  R   CLYQV+YGDGSFTVG
Sbjct: 189 PCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVG 248

Query: 249 EFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMK---ASSFSY 308
           +F TE+L+F     +  VALGCGHDNEGLFVG+AGLLGLG G LS   Q        FSY
Sbjct: 249 DFSTETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSY 308

Query: 309 CLVDRDSSSS-SALEFNSAAPSDTVN-APLLRSGRVDTFYYVGLVGLSVGG-QLLSIPPS 368
           CLVDR +SS  S++ F +AA S      PLL + ++DTFYYVGL+G+SVGG ++  +  S
Sbjct: 309 CLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTAS 368

Query: 369 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 428
           LF++D  G GG+I+DSGT++TRL   AY  +RDAF      L+R    +LFDTC+DLS+ 
Sbjct: 369 LFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNM 428

Query: 429 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 488
           + V +PT+   F G   + LP  NYLIPVD+ G FC AFA T   LSIIGN+QQQG RV 
Sbjct: 429 NEVKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVV 484

Query: 489 FDLANSLLGFSPNKC 496
           +DLA+S +GF+P  C
Sbjct: 489 YDLASSRVGFAPGGC 484

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BLAST of CmaCh13G010350 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 7.8e-107
Identity = 196/424 (46.23%), Postives = 275/424 (64.86%), Query Frame = 0


Query: 76  SSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRSDLQPL 135
           SSS ++L+L  RD   +  ++++   + +R+ RD+ RV ++  R+   +  +  SD    
Sbjct: 55  SSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV--IPSSD---- 114

Query: 136 QTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 195
            +     D  + IVSG  QGSGEYF RIGVG P +  YMV+D+GSD+ W+QCQPC  CY+
Sbjct: 115 -SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYK 174

Query: 196 QTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVTESLSF 255
           Q+DP+FDP  S S+  ++C S  C  +E SGC +  C Y+V YGDGS+T G    E+L+F
Sbjct: 175 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 234

Query: 256 GNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMK---ASSFSYCLVDRDSSS 315
             +  + NVA+GCGH N G+F+G+AGLLG+GGGS+S + Q+      +F YCLV R + S
Sbjct: 235 AKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS 294

Query: 316 SSALEF-NSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAGYGG 375
           + +L F   A P      PL+R+ R  +FYYVGL GL VGG  + +P  +F + + G GG
Sbjct: 295 TGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGG 354

Query: 376 IIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQSRVTIPTLSFQ 435
           +++D+GTA+TRL T AY   RD F S+T  L R +GV++FDTCYDLS    V +PT+SF 
Sbjct: 355 VVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 414

Query: 436 FAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLLGFS 495
           F  G  L LP +N+L+PVD  GT+C AFA + + LSIIGN+QQ+G +V FD AN  +GF 
Sbjct: 415 FTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFG 470

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BLAST of CmaCh13G010350 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.8e-72
Identity = 153/347 (44.09%), Postives = 208/347 (59.94%), Query Frame = 0


Query: 155 GSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLAC 214
           G GEY   + +G PA+PF  ++DTGSD+ W QCQPCT C+ Q+ PIF+P+ SSSF++L C
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 150

Query: 215 ESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEG 274
            S  CQAL +  C    C Y   YGDGS T G   TE+L+FG S +I N+  GCG +N+G
Sbjct: 151 SSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQG 210

Query: 275 LFVGS-AGLLGLGGGSLSLISQMKASSFSYCLVDRDSSSSSALEFNSAAPSDTVNAP--- 334
              G+ AGL+G+G G LSL SQ+  + FSYC+    SS+ S L   S A S T  +P   
Sbjct: 211 FGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTT 270

Query: 335 LLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMD-DAGYGGIIVDSGTAITRLQTQAYN 394
           L++S ++ TFYY+ L GLSVG   L I PS F ++ + G GGII+DSGT +T     AY 
Sbjct: 271 LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQ 330

Query: 395 TLRDAFVSRTPYLRRTNGVALFDTCYDL-SSQSRVTIPTLSFQFAGGKSLQLPPKNYLIP 454
           ++R  F+S+          + FD C+   S  S + IPT    F GG  L+LP +NY I 
Sbjct: 331 SVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS 390

Query: 455 VDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLLGFSPNKC 496
             S G  CLA   ++  +SI GN+QQQ   V +D  NS++ F+  +C
Sbjct: 391 -PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of CmaCh13G010350 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-70
Identity = 166/442 (37.56%), Postives = 231/442 (52.26%), Query Frame = 0


Query: 71  VVPANSSSSFSLQLHSRDALQNAGHKDYK-----------SLVLSRLDRDSSRVKSLNDR 130
           V P +S+S  +L  H +   Q     D +            L+   + R   R++S+N  
Sbjct: 19  VAPTSSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAM 78

Query: 131 LEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTG 190
           L+ S                    + TP+ +    G GEY   + +G P   F  ++DTG
Sbjct: 79  LQSS------------------SGIETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTG 138

Query: 191 SDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYG 250
           SD+ W QC+PCT C+ Q  PIF+P+ SSSF++L CES  CQ L +  C   +C Y   YG
Sbjct: 139 SDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYG 198

Query: 251 DGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGS-AGLLGLGGGSLSLISQMKA 310
           DGS T G   TE+ +F  S ++ N+A GCG DN+G   G+ AGL+G+G G LSL SQ+  
Sbjct: 199 DGSTTQGYMATETFTFETS-SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV 258

Query: 311 SSFSYCLVDRDSSSSSALEFNSAA---PSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLL 370
             FSYC+    SSS S L   SAA   P  + +  L+ S    T+YY+ L G++VGG  L
Sbjct: 259 GQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNL 318

Query: 371 SIPPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCY 430
            IP S FQ+ D G GG+I+DSGT +T L   AYN +  AF  +          +   TC+
Sbjct: 319 GIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCF 378

Query: 431 DLSSQ-SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTS-SLSIIGNVQ 490
              S  S V +P +S QF GG  L L  +N LI   + G  CLA   ++   +SI GN+Q
Sbjct: 379 QQPSDGSTVQVPEISMQFDGG-VLNLGEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQ 435

Query: 491 QQGTRVHFDLANSLLGFSPNKC 496
           QQ T+V +DL N  + F P +C
Sbjct: 439 QQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of CmaCh13G010350 vs. ExPASy TrEMBL
Match: A0A6J1KJJ8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495193 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.4e-278
Identity = 495/495 (100.00%), Postives = 495/495 (100.00%), Query Frame = 0


Query: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60
           MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK
Sbjct: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60

Query: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120
           ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL
Sbjct: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180
           EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS
Sbjct: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD
Sbjct: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300
           GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS
Sbjct: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS
Sbjct: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420
           LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ
Sbjct: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLLGFSPNKC 496
           FDLANSLLGFSPNKC
Sbjct: 481 FDLANSLLGFSPNKC 495

[Close]

BLAST of CmaCh13G010350 vs. ExPASy TrEMBL
Match: A0A6J1E815 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111431581 PE=3 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 2.6e-270
Identity = 483/495 (97.58%), Postives = 488/495 (98.59%), Query Frame = 0


Query: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60
           MATANL PLFSFA FFFSLLPFTLCRTSPELSH SSSSSAFLDVSASLKQANQVLKFDPK
Sbjct: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSH-SSSSSAFLDVSASLKQANQVLKFDPK 60

Query: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120
           ALLSFQQQ+EVVP NSSSSFSLQLHSRDALQNAGH+DYKSLVLSRLDRDSSRVKSLNDRL
Sbjct: 61  ALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180
           EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVG+PAKPFYMVLDTGS
Sbjct: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPRTSSSFASL CESPQCQALETSGCRAAKCLYQVAYGD
Sbjct: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300
           GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASS
Sbjct: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSSALEFNSAAPSD+VNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS
Sbjct: 301 FSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420
           LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNG ALFDTCYDLSSQ
Sbjct: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLLGFSPNKC 496
           FDLANSL+GFSPNKC
Sbjct: 481 FDLANSLVGFSPNKC 494

[Close]

BLAST of CmaCh13G010350 vs. ExPASy TrEMBL
Match: A0A6J1C6K9 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN=LOC111008878 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.9e-242
Identity = 433/485 (89.28%), Postives = 457/485 (94.23%), Query Frame = 0


Query: 12  FAIFFFSL-LPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQNE 71
           F IF FSL LPFT CR+SPELS    SSSA LDVSASLKQA+QVLKFDPK L+SFQQQ +
Sbjct: 6   FTIFIFSLFLPFTSCRSSPELSR---SSSALLDVSASLKQADQVLKFDPKGLMSFQQQEQ 65

Query: 72  VVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRS 131
           +VPAN SSSFSLQLH RDAL+NAGHKDYKSLVLSRLDRDSSRVKSLNDRL+F+LSDLKRS
Sbjct: 66  LVPAN-SSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS 125

Query: 132 DLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQPC 191
           DLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVGQPAKPFYMVLDTGSDINWLQCQPC
Sbjct: 126 DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 185

Query: 192 TDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVT 251
           TDCYQQ+DPIFDPRTSSSFASL CES QCQALE SGCRAAKCLYQV+YGDGSFTVGEFVT
Sbjct: 186 TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT 245

Query: 252 ESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASSFSYCLVDRDS 311
           E+LSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASSFSYCLVDRDS
Sbjct: 246 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 305

Query: 312 SSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAGYG 371
            SSS+L+FNS APSD+V APLLRSGRVDTFYYVGL GLSVGGQLLSIPPSLFQMDD+GYG
Sbjct: 306 GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG 365

Query: 372 GIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQSRVTIPTLSF 431
           GIIVDSGTAITRLQTQAYN+LRDAFV  TPYLR+TNG ALFDTCYDLSSQSRVTIPT+SF
Sbjct: 366 GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF 425

Query: 432 QFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLLGF 491
            F+GGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLANSL+GF
Sbjct: 426 HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF 485

Query: 492 SPNKC 496
           SPNKC
Sbjct: 486 SPNKC 486

[Close]

BLAST of CmaCh13G010350 vs. ExPASy TrEMBL
Match: A0A5D3BI72 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001860 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.1e-240
Identity = 428/495 (86.46%), Postives = 462/495 (93.33%), Query Frame = 0


Query: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60
           MAT+NL  LF   +FFFSL PF L R+S   SH S  SSA LDVSASL+QANQVLKFDP 
Sbjct: 1   MATSNLLTLF---LFFFSLFPFALSRSS---SHLSPHSSASLDVSASLQQANQVLKFDPT 60

Query: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120
           A +SFQQQ  +VP+NSS SFSLQLH RD+L NAGHKDYKSLVLSRL RDSSRVKS+ DRL
Sbjct: 61  ASISFQQQVRLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180
           EF+LS+LKRSDL+PL+TEILPEDLSTPIVSGTSQGSGEYFSR+GVGQPAKPFYMVLDTGS
Sbjct: 121 EFALSELKRSDLEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPR+SSSFASL CES QCQALETSGCRA+KCLYQV+YGD
Sbjct: 181 DINWLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300
           GSFTVGEFVTE+L+FGNSG I NVA+GCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASS
Sbjct: 241 GSFTVGEFVTETLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSS LEFNSAAP+D+VNAPLL+SGRVDTFYYVGL G+SVGGQLLSIPP+
Sbjct: 301 FSYCLVDRDSSSSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPN 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420
           LFQMDD+GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYL++TNG ALFDTCYDLSSQ
Sbjct: 361 LFQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPT+SF+FAGGKSLQLPPKNYLIPVDSVGTFC AFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLLGFSPNKC 496
           +DLANS++GFSP+KC
Sbjct: 481 YDLANSVVGFSPHKC 489

[Close]

BLAST of CmaCh13G010350 vs. ExPASy TrEMBL
Match: A0A1S3C5F6 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo OX=3656 GN=LOC103496838 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.1e-240
Identity = 428/495 (86.46%), Postives = 462/495 (93.33%), Query Frame = 0


Query: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60
           MAT+NL  LF   +FFFSL PF L R+S   SH S  SSA LDVSASL+QANQVLKFDP 
Sbjct: 48  MATSNLLTLF---LFFFSLFPFALSRSS---SHLSPHSSASLDVSASLQQANQVLKFDPT 107

Query: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120
           A +SFQQQ  +VP+NSS SFSLQLH RD+L NAGHKDYKSLVLSRL RDSSRVKS+ DRL
Sbjct: 108 ASISFQQQVRLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRL 167

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180
           EF+LS+LKRSDL+PL+TEILPEDLSTPIVSGTSQGSGEYFSR+GVGQPAKPFYMVLDTGS
Sbjct: 168 EFALSELKRSDLEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGS 227

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPR+SSSFASL CES QCQALETSGCRA+KCLYQV+YGD
Sbjct: 228 DINWLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGD 287

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300
           GSFTVGEFVTE+L+FGNSG I NVA+GCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASS
Sbjct: 288 GSFTVGEFVTETLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 347

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSS LEFNSAAP+D+VNAPLL+SGRVDTFYYVGL G+SVGGQLLSIPP+
Sbjct: 348 FSYCLVDRDSSSSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPN 407

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420
           LFQMDD+GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYL++TNG ALFDTCYDLSSQ
Sbjct: 408 LFQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQ 467

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPT+SF+FAGGKSLQLPPKNYLIPVDSVGTFC AFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 468 SRVTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVH 527

Query: 481 FDLANSLLGFSPNKC 496
           +DLANS++GFSP+KC
Sbjct: 528 YDLANSVVGFSPHKC 536

[Close]

BLAST of CmaCh13G010350 vs. NCBI nr
Match: XP_023000890.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 966.5 bits (2497), Expect = 9.0e-278
Identity = 495/495 (100.00%), Postives = 495/495 (100.00%), Query Frame = 0


Query: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60
           MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK
Sbjct: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60

Query: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120
           ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL
Sbjct: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180
           EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS
Sbjct: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD
Sbjct: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300
           GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS
Sbjct: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS
Sbjct: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420
           LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ
Sbjct: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLLGFSPNKC 496
           FDLANSLLGFSPNKC
Sbjct: 481 FDLANSLLGFSPNKC 495

[Close]

BLAST of CmaCh13G010350 vs. NCBI nr
Match: XP_023519500.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 947.2 bits (2447), Expect = 5.7e-272
Identity = 484/495 (97.78%), Postives = 489/495 (98.79%), Query Frame = 0


Query: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60
           MATANL PLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK
Sbjct: 1   MATANLLPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60

Query: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120
           ALLSFQQQ+EVVP NSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL
Sbjct: 61  ALLSFQQQDEVVPGNSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180
           EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVG+PAKPFYMVLDTGS
Sbjct: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPRTSSSFASL CESPQCQALETSGCRAAKCLYQVAYGD
Sbjct: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300
           GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASS
Sbjct: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSSALEFNSAAPSD+VNAPLL+SGRVDTFYYVGLVGLSVGGQLLSIPPS
Sbjct: 301 FSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLKSGRVDTFYYVGLVGLSVGGQLLSIPPS 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420
           LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNG ALFDTCYDLSSQ
Sbjct: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPTLSFQFAGG SLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTLSFQFAGGNSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLLGFSPNKC 496
           FDLANSL+GFSPNKC
Sbjct: 481 FDLANSLVGFSPNKC 495

[Close]

BLAST of CmaCh13G010350 vs. NCBI nr
Match: KAG6584369.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019953.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 944.5 bits (2440), Expect = 3.7e-271
Identity = 486/498 (97.59%), Postives = 490/498 (98.39%), Query Frame = 0


Query: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSH---SSSSSSAFLDVSASLKQANQVLKF 60
           MATANL PLFSFAIFFFSLLPFTLCRTSPELSH   SSSSSSAFLDVSASLKQANQVLKF
Sbjct: 1   MATANLLPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSSSSAFLDVSASLKQANQVLKF 60

Query: 61  DPKALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLN 120
           DPKALLSFQQQ+EVVP NSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLN
Sbjct: 61  DPKALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLN 120

Query: 121 DRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLD 180
           DRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLD
Sbjct: 121 DRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLD 180

Query: 181 TGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVA 240
           TGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASL CESPQCQALETSGCRAAKCLYQVA
Sbjct: 181 TGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVA 240

Query: 241 YGDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMK 300
           YGDGSFTVGEFVTESLSFGNSGTI+NVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMK
Sbjct: 241 YGDGSFTVGEFVTESLSFGNSGTISNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK 300

Query: 301 ASSFSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSI 360
           ASSFSYCLVDRDSSSSSALEFNSAAPSD+VNAPLLRSGRVDTFYYVGLVGLSVGGQLLSI
Sbjct: 301 ASSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSI 360

Query: 361 PPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDL 420
           PPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNG ALFDTCYDL
Sbjct: 361 PPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDL 420

Query: 421 SSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGT 480
           SSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGT
Sbjct: 421 SSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGT 480

Query: 481 RVHFDLANSLLGFSPNKC 496
           RVHFDLANSL+GFSPNKC
Sbjct: 481 RVHFDLANSLVGFSPNKC 498

[Close]

BLAST of CmaCh13G010350 vs. NCBI nr
Match: XP_022924034.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata])

HSP 1 Score: 940.6 bits (2430), Expect = 5.3e-270
Identity = 483/495 (97.58%), Postives = 488/495 (98.59%), Query Frame = 0


Query: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60
           MATANL PLFSFA FFFSLLPFTLCRTSPELSH SSSSSAFLDVSASLKQANQVLKFDPK
Sbjct: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSH-SSSSSAFLDVSASLKQANQVLKFDPK 60

Query: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120
           ALLSFQQQ+EVVP NSSSSFSLQLHSRDALQNAGH+DYKSLVLSRLDRDSSRVKSLNDRL
Sbjct: 61  ALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180
           EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVG+PAKPFYMVLDTGS
Sbjct: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTDPIFDPRTSSSFASL CESPQCQALETSGCRAAKCLYQVAYGD
Sbjct: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300
           GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASS
Sbjct: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSSALEFNSAAPSD+VNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS
Sbjct: 301 FSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420
           LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNG ALFDTCYDLSSQ
Sbjct: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLLGFSPNKC 496
           FDLANSL+GFSPNKC
Sbjct: 481 FDLANSLVGFSPNKC 494

[Close]

BLAST of CmaCh13G010350 vs. NCBI nr
Match: XP_038894046.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Benincasa hispida])

HSP 1 Score: 864.8 bits (2233), Expect = 3.7e-247
Identity = 439/495 (88.69%), Postives = 467/495 (94.34%), Query Frame = 0


Query: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPK 60
           MAT NL PLF+ A+FFFSL PFTL RTSPELS  SSSSSA LDVSASL+QAN VLKFDPK
Sbjct: 1   MATPNLPPLFAVALFFFSLFPFTLGRTSPELSR-SSSSSASLDVSASLQQANHVLKFDPK 60

Query: 61  ALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRL 120
             +SFQQQ +VVP+NSS SFSLQLH RDAL NAGHKDYK+LVLSRL RDSSRVKSL DRL
Sbjct: 61  PFMSFQQQVQVVPSNSSFSFSLQLHPRDALHNAGHKDYKTLVLSRLVRDSSRVKSLYDRL 120

Query: 121 EFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 180
           +F+LS+LK SDL PL+TEILPEDLSTPIVSGTSQGSGEYFSR+GVGQPAKPFYMVLDTGS
Sbjct: 121 QFALSELKSSDLHPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGS 180

Query: 181 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 240
           DINWLQCQPCTDCYQQTD IFDPRTSS+FASL CES QCQALETSGCRA+KCLYQV+YGD
Sbjct: 181 DINWLQCQPCTDCYQQTDSIFDPRTSSTFASLPCESQQCQALETSGCRASKCLYQVSYGD 240

Query: 241 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 300
           GSFTVGEFVTE+LSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASS
Sbjct: 241 GSFTVGEFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASS 300

Query: 301 FSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPS 360
           FSYCLVDRDSSSSS LEFNSAA SD+VNAPLL+SGRVDTFYY+GL+GLSVGGQ+LSIPP+
Sbjct: 301 FSYCLVDRDSSSSSFLEFNSAASSDSVNAPLLKSGRVDTFYYIGLIGLSVGGQILSIPPN 360

Query: 361 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 420
           LFQMDD+GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYL++TNG ALFDTCYDLSSQ
Sbjct: 361 LFQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQ 420

Query: 421 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 480
           SRVTIPT+SFQFAGGKSLQLPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRVH
Sbjct: 421 SRVTIPTVSFQFAGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVH 480

Query: 481 FDLANSLLGFSPNKC 496
           FDLANS++GFSPNKC
Sbjct: 481 FDLANSVVGFSPNKC 494

[Close]

BLAST of CmaCh13G010350 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 569.3 bits (1466), Expect = 3.0e-162
Identity = 291/497 (58.55%), Postives = 371/497 (74.65%), Query Frame = 0


Query: 8   PLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDP--KALLSF 67
           P F   +   +L  F     +   S S+   +  LDV +SL+Q   +L  DP   +L + 
Sbjct: 4   PRFLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTT 63

Query: 68  QQQNEVVPA--NSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFS 127
           + ++   P   NSSS  SL+LHSRD    + HKDYKSL LSRL+RDSSRV  +  ++ F+
Sbjct: 64  KPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFA 123

Query: 128 LSDLKRSDLQPL---QTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGS 187
           +  + RSDL+P+    T    EDL+TP+VSG SQGSGEYFSRIGVG PAK  Y+VLDTGS
Sbjct: 124 VEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGS 183

Query: 188 DINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGD 247
           D+NW+QC+PC DCYQQ+DP+F+P +SS++ SL C +PQC  LETS CR+ KCLYQV+YGD
Sbjct: 184 DVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGD 243

Query: 248 GSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASS 307
           GSFTVGE  T++++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+ +QMKA+S
Sbjct: 244 GSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATS 303

Query: 308 FSYCLVDRDSSSSSALEFNSA-APSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPP 367
           FSYCLVDRDS  SS+L+FNS         APLLR+ ++DTFYYVGL G SVGG+ + +P 
Sbjct: 304 FSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPD 363

Query: 368 SLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRR-TNGVALFDTCYDLS 427
           ++F +D +G GG+I+D GTA+TRLQTQAYN+LRDAF+  T  L++ ++ ++LFDTCYD S
Sbjct: 364 AIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFS 423

Query: 428 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 487
           S S V +PT++F F GGKSL LP KNYLIPVD  GTFC AFAPT+SSLSIIGNVQQQGTR
Sbjct: 424 SLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTR 483

Query: 488 VHFDLANSLLGFSPNKC 496
           + +DL+ +++G S NKC
Sbjct: 484 ITYDLSKNVIGLSGNKC 500

[Close]

BLAST of CmaCh13G010350 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 565.8 bits (1457), Expect = 3.3e-161
Identity = 284/488 (58.20%), Postives = 374/488 (76.64%), Query Frame = 0


Query: 10  FSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQN 69
           F F IFF +       R  PE   +S+++++ L+V+ S+ +      F          Q 
Sbjct: 7   FFFFIFFLTSHSSVFSRILPE---TSTTTTSILNVADSIHRTKYTSSFR-------LNQQ 66

Query: 70  EVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKR 129
           E    ++SSSFSLQLHSR +++   H DYKSL L+RL+RD++RVKSL  RL+ +++++ +
Sbjct: 67  EEQTHSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISK 126

Query: 130 SDLQPLQTEILPE--DLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQC 189
           +DL+P+ T    E  D+  P++SGT+QGSGEYF+R+G+G+PA+  YMVLDTGSD+NWLQC
Sbjct: 127 ADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQC 186

Query: 190 QPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGDGSFTVGE 249
            PC DCY QT+PIF+P +SSS+  L+C++PQC ALE S CR A CLY+V+YGDGS+TVG+
Sbjct: 187 TPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGD 246

Query: 250 FVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASSFSYCLVD 309
           F TE+L+ G S  + NVA+GCGH NEGLFVG+AGLLGLGGG L+L SQ+  +SFSYCLVD
Sbjct: 247 FATETLTIG-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVD 306

Query: 310 RDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDA 369
           RDS S+S ++F ++   D V APLLR+ ++DTFYY+GL G+SVGG+LL IP S F+MD++
Sbjct: 307 RDSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDES 366

Query: 370 GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQSRVTIPT 429
           G GGII+DSGTA+TRLQT+ YN+LRD+FV  T  L +  GVA+FDTCY+LS+++ V +PT
Sbjct: 367 GSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPT 426

Query: 430 LSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSL 489
           ++F F GGK L LP KNY+IPVDSVGTFCLAFAPT SSL+IIGNVQQQGTRV FDLANSL
Sbjct: 427 VAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSL 483

Query: 490 LGFSPNKC 496
           +GFS NKC
Sbjct: 487 IGFSSNKC 483

[Close]

BLAST of CmaCh13G010350 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 395.6 bits (1015), Expect = 5.9e-110
Identity = 240/495 (48.48%), Postives = 310/495 (62.63%), Query Frame = 0


Query: 9   LFSFAIFFFSLLPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQ 68
           LFS   FF SL  F+   +   L  +S S      VS            D ++LL  + +
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDS-------DSESLLESEFE 68

Query: 69  NEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLK 128
           +    + SSSS +L L   DAL  + +K    L  SRL RDS RVKS+        +   
Sbjct: 69  SG-SDSESSSSITLNLDHIDAL--SSNKTPDELFSSRLQRDSRRVKSI-------ATLAA 128

Query: 129 RSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQ 188
           +   + +     P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC 
Sbjct: 129 QIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA 188

Query: 189 PCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGC--RAAKCLYQVAYGDGSFTVG 248
           PC  CY Q+DPIFDPR S ++A++ C SP C+ L+++GC  R   CLYQV+YGDGSFTVG
Sbjct: 189 PCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVG 248

Query: 249 EFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMK---ASSFSY 308
           +F TE+L+F     +  VALGCGHDNEGLFVG+AGLLGLG G LS   Q        FSY
Sbjct: 249 DFSTETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSY 308

Query: 309 CLVDRDSSSS-SALEFNSAAPSDTVN-APLLRSGRVDTFYYVGLVGLSVGG-QLLSIPPS 368
           CLVDR +SS  S++ F +AA S      PLL + ++DTFYYVGL+G+SVGG ++  +  S
Sbjct: 309 CLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTAS 368

Query: 369 LFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQ 428
           LF++D  G GG+I+DSGT++TRL   AY  +RDAF      L+R    +LFDTC+DLS+ 
Sbjct: 369 LFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNM 428

Query: 429 SRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVH 488
           + V +PT+   F G   + LP  NYLIPVD+ G FC AFA T   LSIIGN+QQQG RV 
Sbjct: 429 NEVKVPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVV 484

Query: 489 FDLANSLLGFSPNKC 496
           +DLA+S +GF+P  C
Sbjct: 489 YDLASSRVGFAPGGC 484

[Close]

BLAST of CmaCh13G010350 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 389.0 bits (998), Expect = 5.5e-108
Identity = 196/424 (46.23%), Postives = 275/424 (64.86%), Query Frame = 0


Query: 76  SSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRSDLQPL 135
           SSS ++L+L  RD   +  ++++   + +R+ RD+ RV ++  R+   +  +  SD    
Sbjct: 55  SSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV--IPSSD---- 114

Query: 136 QTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 195
            +     D  + IVSG  QGSGEYF RIGVG P +  YMV+D+GSD+ W+QCQPC  CY+
Sbjct: 115 -SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYK 174

Query: 196 QTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVTESLSF 255
           Q+DP+FDP  S S+  ++C S  C  +E SGC +  C Y+V YGDGS+T G    E+L+F
Sbjct: 175 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 234

Query: 256 GNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMK---ASSFSYCLVDRDSSS 315
             +  + NVA+GCGH N G+F+G+AGLLG+GGGS+S + Q+      +F YCLV R + S
Sbjct: 235 AKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS 294

Query: 316 SSALEF-NSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAGYGG 375
           + +L F   A P      PL+R+ R  +FYYVGL GL VGG  + +P  +F + + G GG
Sbjct: 295 TGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGG 354

Query: 376 IIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQSRVTIPTLSFQ 435
           +++D+GTA+TRL T AY   RD F S+T  L R +GV++FDTCYDLS    V +PT+SF 
Sbjct: 355 VVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 414

Query: 436 FAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLLGFS 495
           F  G  L LP +N+L+PVD  GT+C AFA + + LSIIGN+QQ+G +V FD AN  +GF 
Sbjct: 415 FTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFG 470

[Close]

BLAST of CmaCh13G010350 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 381.3 bits (978), Expect = 1.2e-105
Identity = 226/479 (47.18%), Postives = 298/479 (62.21%), Query Frame = 0


Query: 31  LSHSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQNEVVPANSSSSFSLQLHSRDAL 90
           L  +SS+SS +  +  +   ++  L +     L+ +  +E     S++S S+ L   DAL
Sbjct: 17  LFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSE-----STTSLSVHLSHVDAL 76

Query: 91  QNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVS 150
            +        L   RL RDS RVKS+      SL+ +        +T       S  ++S
Sbjct: 77  SSFSDASPADLFNLRLQRDSLRVKSIT-----SLAAVSTGRNATKRTPRTAGGFSGAVIS 136

Query: 151 GTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFA 210
           G SQGSGEYF R+GVG PA   YMVLDTGSD+ WLQC PC  CY QTD IFDP+ S +FA
Sbjct: 137 GLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFA 196

Query: 211 SLACESPQCQAL-ETSGC---RAAKCLYQVAYGDGSFTVGEFVTESLSFGNSGTINNVAL 270
           ++ C S  C+ L ++S C   R+  CLYQV+YGDGSFT G+F TE+L+F +   +++V L
Sbjct: 197 TVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTF-HGARVDHVPL 256

Query: 271 GCGHDNEGLFVGSAGLLGLGGGSLSLISQMK---ASSFSYCLVDRDSSSSSA------LE 330
           GCGHDNEGLFVG+AGLLGLG G LS  SQ K      FSYCLVDR SS SS+      + 
Sbjct: 257 GCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVF 316

Query: 331 FNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGG-QLLSIPPSLFQMDDAGYGGIIVDS 390
            N+A P  +V  PLL + ++DTFYY+ L+G+SVGG ++  +  S F++D  G GG+I+DS
Sbjct: 317 GNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDS 376

Query: 391 GTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQSRVTIPTLSFQFAGGK 450
           GT++TRL   AY  LRDAF      L+R    +LFDTC+DLS  + V +PT+ F F GG+
Sbjct: 377 GTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGE 436

Query: 451 SLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLLGFSPNKC 496
            + LP  NYLIPV++ G FC AFA T  SLSIIGN+QQQG RV +DL  S +GF    C
Sbjct: 437 -VSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:

BLAST of CmaCh13G010350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LS40	4.2e-161	58.55	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	8.3e-109	48.48	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	7.8e-107	46.23	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q766C3	2.8e-72	44.09	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.2e-70	37.56	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of CmaCh13G010350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KJJ8	4.4e-278	100.00	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1E815	2.6e-270	97.58	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1C6K9	3.9e-242	89.28	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN...	[more]
A0A5D3BI72	2.1e-240	86.46	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3C5F6	2.1e-240	86.46	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]

BLAST of CmaCh13G010350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000890.1	9.0e-278	100.00	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima]	[more]
XP_023519500.1	5.7e-272	97.78	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6584369.1	3.7e-271	97.59	Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022924034.1	5.3e-270	97.58	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata]	[more]
XP_038894046.1	3.7e-247	88.69	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Benincasa hispida]	[more]

BLAST of CmaCh13G010350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18490.1	3.0e-162	58.55	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	3.3e-161	58.20	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	5.9e-110	48.48	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	5.5e-108	46.23	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.2e-105	47.18	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 340..491 e-value: 1.2E-37 score: 129.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 318..495 e-value: 9.1E-52 score: 177.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 134..317 e-value: 1.4E-50 score: 173.9
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 152..495
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 159..318 e-value: 3.0E-48 score: 164.4
None	No IPR available	PANTHER	PTHR13683:SF274	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1	coord: 15..495
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 15..495
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 174..185
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 159..491 score: 48.089264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh13G010350.1	CmaCh13G010350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity