CmaCh13G007930 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh13G007930 vs. ExPASy Swiss-Prot
Match: Q84P23 (4-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana OX=3702 GN=4CLL9 PE=1 SV=2)

HSP 1 Score: 658.7 bits (1698), Expect = 5.9e-188
Identity = 352/565 (62.30%), Postives = 434/565 (76.81%), Query Frame = 0

Query: 9   NAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNA------ 68
           N +  +D  SGF  +T I+ SLRP L +PP+ QPLS  E ALSLL  S PP+ A      
Sbjct: 6   NDSRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEA 65

Query: 69  -AVLVDSNSGVHLSYAIFLRQIRTLASNLK-ALTSLSNGQVAFILAPTSLEVPVLYFALL 128
              LV+S+SG +L+Y   LR++R+LA +L+    SL++  VAFIL+P+SL++PVLY AL+
Sbjct: 66  LTYLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALM 125

Query: 129 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKL--PKLQLGTVLIDSPAFLSM 188
           S+GV++SPANPIGS+SE+SHQ+ +SEPVIAFATS T  KL    L LGTVL+DS  FLS 
Sbjct: 126 SIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSW 185

Query: 189 MTEINRSDGLA----DVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVN--SSPNPLL 248
              +NRSD  +     V+V+QSD AAIL+SSGTTGRVKGVLL+HRNLI     S    L 
Sbjct: 186 ---LNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQ 245

Query: 249 SEVNNGETEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVT 308
             VN       +V L  LPLFHVFGF+M++RAIS GETLVL+ +F+   M  AVEK++VT
Sbjct: 246 DPVNYD-----RVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVT 305

Query: 309 YIPVSPPLVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 368
            +PVSPPL+VAL KSEL  KYDL SL+ LGCGGAPLGK++ E+F QK P V+I+QGYGLT
Sbjct: 306 GMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLT 365

Query: 369 ESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGY 428
           ES+  AA + GPEE     SVGR+SENMEA IVDP++GE+LPPG  GELWLRGP IMKGY
Sbjct: 366 ESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGY 425

Query: 429 VGDADATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 488
           VG+  A+ ET+  +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN
Sbjct: 426 VGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSN 485

Query: 489 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAI 548
           P++IDAAV+P+PDEDAGEIPMA++VRKPGSN+ EAQ+IDF+AKQV PYKK+RRV+FI+AI
Sbjct: 486 PDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAI 545

Query: 549 PKSPAGKILRRELTQHARSHGSNKL 558
           PK+PAGKILRRELT+ A    ++KL
Sbjct: 546 PKNPAGKILRRELTKIAVDGNASKL 562

BLAST of CmaCh13G007930 vs. ExPASy Swiss-Prot
Match: Q7F1X5 (4-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL5 PE=2 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 6.0e-156
Identity = 300/565 (53.10%), Postives = 392/565 (69.38%), Query Frame = 0

Query: 1   MADRNPSLNAAHF-VDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
           MADR P        +D RSGFC  T+IF S R P  +PP S P++   +A SLL SS  P
Sbjct: 1   MADRAPPPPPPPAGIDSRSGFCAATRIFHSTRAPGDLPPESLPMTAAAYAFSLLSSSTLP 60

Query: 61  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
              A LVD+ +G+ +SY  FL  +R+LA  L     L  G VA ++AP+ LEVPVL FAL
Sbjct: 61  GRPA-LVDAATGIAISYPSFLAAVRSLAGGLWCSLGLRPGDVALVVAPSRLEVPVLDFAL 120

Query: 121 LSLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSM- 180
           +S+G ++SPANP+ +  E +HQ+ LS PV+AFA    A+KLP+  +  V+I S  +  + 
Sbjct: 121 MSIGAVVSPANPVSTPEEYAHQVALSRPVVAFAAPEVAAKLPE-HVRCVVIGSDEYGRLA 180

Query: 181 MTEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNG 240
            ++  R+   A V V QSD+AA+LYSSGTTGRVK V ++HRNLI + S       +V   
Sbjct: 181 ASDGRRAAAPAAVAVKQSDTAAVLYSSGTTGRVKAVAITHRNLIALMSLHADNREKVARE 240

Query: 241 ETE------PRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVT 300
             E      P  V+L  +PLFHVFGF+M+LR++S GET VLM++FDF+  L A+E++RVT
Sbjct: 241 AAEAGEEPPPPAVTLLPIPLFHVFGFMMVLRSVSMGETSVLMERFDFIAALRAIERYRVT 300

Query: 301 YIPVSPPLVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 360
            +P +PP++VA+ K E   + DLSSL V+G GGAPLG+EV E+F    P VE++QGYGLT
Sbjct: 301 LLPAAPPVLVAMVKYEEARRRDLSSLLVIGIGGAPLGREVAEQFASVFPNVELVQGYGLT 360

Query: 361 ESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGY 420
           ES+   A ++GPEES    SVG+L  +++A IVDP++                     GY
Sbjct: 361 ESSGAVAATVGPEESKAYGSVGKLGSHLQAKIVDPST---------------------GY 420

Query: 421 VGDADATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 480
           VGD +AT  T+  +GWLKTGDLCYF+ DGFLYIVDRLKELIKYK YQVPPAELEH+LQS+
Sbjct: 421 VGDDEATAATVDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQVPPAELEHILQSH 480

Query: 481 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAI 540
           P I DAAVIPYPDE+AGE+PMA++VR+PGSN+T+ QV+D++AKQVAPYKK+RRV+F+ AI
Sbjct: 481 PGIADAAVIPYPDEEAGELPMAFIVRQPGSNITKEQVMDYVAKQVAPYKKVRRVAFVTAI 540

Query: 541 PKSPAGKILRRELTQHARSHGSNKL 558
           PKSPAGKILRREL Q A S G++KL
Sbjct: 541 PKSPAGKILRRELVQQALSMGASKL 542

BLAST of CmaCh13G007930 vs. ExPASy Swiss-Prot
Match: Q8RU95 (4-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 546.2 bits (1406), Expect = 4.3e-154
Identity = 293/558 (52.51%), Postives = 380/558 (68.10%), Query Frame = 0

Query: 17  RSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPP---PSNAAVLVDSNSGVH 76
           RSGFC  T+ F SLR    +PP   PL+V  +A SLL S+PP         LVD+ +G+ 
Sbjct: 41  RSGFCAATRTFHSLRSVGPLPPEELPLTVAAYAFSLLSSAPPLVVAGRGPALVDAATGIA 100

Query: 77  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVIISPANPIG 136
           +SY  F+ ++R LA  L     L  G VA +++P+ L+V VLYFAL+S+GV++SPANP  
Sbjct: 101 VSYPAFVARVRFLAGGLWCSLGLRPGDVALVVSPSCLDVAVLYFALMSIGVVVSPANPAS 160

Query: 137 SDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMTEINRSDGLA---D 196
           +  E +HQ+RLS P IAF     A++LP+  +  V+I S  F   +   + + G A    
Sbjct: 161 TADEYAHQVRLSRPAIAFVAPEVAARLPR-HVSRVVIGSEVF-DRLASASAAGGWAAPPA 220

Query: 197 VKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNN------------- 256
           V + Q  +AA+LYSSGTTGRVK V ++HRNLI   S+ N +   V               
Sbjct: 221 VAMKQPSTAALLYSSGTTGRVKAVAITHRNLIAQISAYNAIRETVAREAATDAGKGKPPP 280

Query: 257 -GETEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPV 316
              + P  V+L  LPLFHV GF +L R IS GET V+M++FD      AVE++RVT +  
Sbjct: 281 PSPSPPAAVTLFPLPLFHVMGFGLLTRTISSGETAVVMRRFDLAAAARAVERYRVTKLSA 340

Query: 317 SPPLVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTA 376
           +PP+VVAL KS+   + DLSSL  +  GGAPLG+EV ++F    P V+I+Q YGLTEST 
Sbjct: 341 APPVVVALTKSDEARRRDLSSLVAIVVGGAPLGREVSQRFATVFPSVQIVQSYGLTESTG 400

Query: 377 GAARSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDA 436
             A   GPEES+   SVGRL+  ++A IVD A+GE L PG RGELW+RGP +MKGYVGD 
Sbjct: 401 PVATMAGPEESAAYGSVGRLAPRVQAKIVDTATGEVLGPGRRGELWIRGPVVMKGYVGDP 460

Query: 437 DATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEII 496
           +AT  T+ PDGWLKTGDLCYF+ DG+LY+VDRLKELIKYK YQVPPAELEH+LQS PEI 
Sbjct: 461 EATAATITPDGWLKTGDLCYFNEDGYLYVVDRLKELIKYKGYQVPPAELEHILQSRPEIA 520

Query: 497 DAAVIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSP 555
           DAAV+PYPDE+AG++PMA+VVR+PG+ +TE QV++ +AK VAPYKK+RRV+F++AIPKSP
Sbjct: 521 DAAVVPYPDEEAGQLPMAFVVRQPGAYLTEQQVMNCVAKHVAPYKKVRRVAFVNAIPKSP 580

BLAST of CmaCh13G007930 vs. ExPASy Swiss-Prot
Match: Q69RG7 (4-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL7 PE=2 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 5.3e-136
Identity = 265/547 (48.45%), Postives = 367/547 (67.09%), Query Frame = 0

Query: 17  RSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAV------LVDSNS 76
           R+G+C  TK FRSLRPP+ +PP   PLS  E A SLL  S   S++++      LVD+ +
Sbjct: 16  RTGYCAATKSFRSLRPPVPLPPPDVPLSFPEFAFSLLPRSSSSSSSSLLPANPALVDAAT 75

Query: 77  GVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVIISPAN 136
           G  +S+  FL ++R LA  L++   L  G VAF+LAP  L+VPVLYFALLS+G ++SPAN
Sbjct: 76  GEAVSFQAFLSRVRALAGALRSRVGLRGGDVAFVLAPAGLDVPVLYFALLSIGAVVSPAN 135

Query: 137 PIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMTEINRSDG--- 196
           P  + +E+S  + LS   +AFA SSTA+KLP      VL+DSP F S++ +  ++ G   
Sbjct: 136 PALTPAEVSRLVSLSGASVAFAVSSTATKLPAGLTTVVLLDSPHFRSLLMDCGQAQGQEP 195

Query: 197 LADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPRQVSL 256
           L  V V QS++AAI YSSGTTGRVK   L HR+ I + +  + L ++     T      L
Sbjct: 196 LPVVVVRQSETAAIQYSSGTTGRVKAAALPHRSFIAMVAGFHALRAKAREVRT------L 255

Query: 257 CLLPLFHVFGFVMLLRAISRGETLVL----MQKFDFVEMLSAVEKFRVTYIPVSPPLVVA 316
              P+FH  GF+ +L+ ++ G T V+    + +     ++ A E++ V  +  SPP+V+ 
Sbjct: 256 LGAPMFHSMGFLFVLQGVALGATTVVVTDAVARAGIRGLVEAAERWAVMDMTASPPVVLG 315

Query: 317 LAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAARSMG 376
           + K     +  L +L+ + CGGAPL    IE+F ++ P V++  GYG TE+  G +R + 
Sbjct: 316 MTKQ----RCRLPALERITCGGAPLPAPAIERFRRRFPHVDLCMGYGSTEA-GGISRMIS 375

Query: 377 PEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATTETL 436
            EE ++  S GR++EN+E  IVD  +G+ LP G +GELW+RGP +M GYVGD +A   T 
Sbjct: 376 QEECNHIGSAGRVTENVEVKIVDHVTGKPLPAGQQGELWVRGPAVMTGYVGDNEANATTF 435

Query: 437 HPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPY 496
           + +GWLKTGDLCY D DGFL++VDRLKELIKYKAYQVPPAELE +L S P+I+DAAV+PY
Sbjct: 436 NSEGWLKTGDLCYIDQDGFLFVVDRLKELIKYKAYQVPPAELELVLHSLPQIVDAAVMPY 495

Query: 497 PDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRR 551
           P E+AG+IP+A VV++PGS +TEA+V+  +AKQVAPYKKIR+V F+D+IPKSP+GKILRR
Sbjct: 496 PHEEAGQIPVALVVKQPGSKLTEAEVMYNVAKQVAPYKKIRKVLFVDSIPKSPSGKILRR 551

BLAST of CmaCh13G007930 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 2.4e-128
Identity = 259/553 (46.84%), Postives = 359/553 (64.92%), Query Frame = 0

Query: 14  VDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           VD RSG+C    IF S R P+ +P  +  + VT        SS         +D+ +G H
Sbjct: 9   VDERSGYCKSNSIFYSKREPVQLPQ-NHSVDVTTFI-----SSRAHHGKIAFIDAATGRH 68

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVIISPANPIG 133
           L++    R + ++A+ L A+  +  G V  +L+P S+  PV+  A++SLG II+  NP+ 
Sbjct: 69  LTFPQLWRAVDSVATCLSAM-GIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLN 128

Query: 134 SDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMTEINRSDGLADV-- 193
           +  EI+ QI  S+PV+AF      SK+    L  V+ID     S+   +N    L ++  
Sbjct: 129 TPREIAKQITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMR 188

Query: 194 ----------KVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETE 253
                     +V+Q D+A +LYSSGTTG  KGV+ SH+NLI +  +   +LS    G  +
Sbjct: 189 KEPSPNRIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQT---ILSRF--GTED 248

Query: 254 PRQVSLCLLPLFHVFGF-VMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 313
                +C +P+FH++G     +  +S G T+V++ KF+  EMLSA+EK+R TY+P+ PP+
Sbjct: 249 GEHTFICTVPMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPI 308

Query: 314 VVALAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGA 373
           ++AL K  + + AKYDLSSLQ +  GGAPL KEVIE F +  P V I+QGYGLTEST   
Sbjct: 309 LMALLKNANHIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIG 368

Query: 374 ARSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADA 433
           A +   +ES    + G LS +MEA IV+P +GEAL     GELWLRGP IMKGY  + +A
Sbjct: 369 ASTDCLQESRRYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEA 428

Query: 434 TTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDA 493
           T+ T+  +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL S+PEI DA
Sbjct: 429 TSSTIDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDA 488

Query: 494 AVIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAG 552
           AVIPYPD++AG+ PMAYVVRK GSN++E+ V+DFIAK VAPYK+IR+V+F+ +IPK+P+G
Sbjct: 489 AVIPYPDKEAGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSG 548

BLAST of CmaCh13G007930 vs. ExPASy TrEMBL
Match: A0A6J1KJC1 (4-coumarate--CoA ligase-like 9 OS=Cucurbita maxima OX=3661 GN=LOC111495139 PE=4 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180
           SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of CmaCh13G007930 vs. ExPASy TrEMBL
Match: A0A6J1EA56 (4-coumarate--CoA ligase-like 9 OS=Cucurbita moschata OX=3662 GN=LOC111431280 PE=4 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 1.0e-307
Identity = 549/557 (98.56%), Postives = 553/557 (99.28%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180
           SLGV+ISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSKPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDP SGEALPPGHRGELWLRGPGIMKGYVGDA ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of CmaCh13G007930 vs. ExPASy TrEMBL
Match: A0A1S4DV63 (4-coumarate--CoA ligase-like 9 OS=Cucumis melo OX=3656 GN=LOC103489008 PE=4 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 1.9e-261
Identity = 466/558 (83.51%), Postives = 506/558 (90.68%), Query Frame = 0

Query: 1   MADRNPSLNAA-HFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
           MA RN + NAA H +DLRSGFCP TKIF SLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
            N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMM 180
           LSLGV++SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L+ GTV+IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
           TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
            +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
           LVVA+AKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADAT 420
           R+M PEE SNTKSVGRLS +MEA IVDPASGEAL P H+GELWLRGP IMKGYVGD  AT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540
           VIPYPDE+AGEIPMAYVVRKPGSN++EAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

Query: 541 ILRRELTQHARSHGSNKL 558
           ILRREL +HA SHGS+KL
Sbjct: 541 ILRRELAKHALSHGSSKL 558

BLAST of CmaCh13G007930 vs. ExPASy TrEMBL
Match: A0A5D3BKJ5 (4-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004520 PE=4 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 6.7e-259
Identity = 466/569 (81.90%), Postives = 506/569 (88.93%), Query Frame = 0

Query: 1   MADRNPSLNAA-HFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
           MA RN + NAA H +DLRSGFCP TKIF SLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
            N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMM 180
           LSLGV++SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L+ GTV+IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
           TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
            +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
           LVVA+AKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADAT 420
           R+M PEE SNTKSVGRLS +MEA IVDPASGEAL P H+GELWLRGP IMKGYVGD  AT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
           VIPYPDE+AGEIPMAYVVRKPGSN++EAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 540

Query: 541 IDAIPKSPAGKILRRELTQHARSHGSNKL 558
           IDAIPKSPAGKILRREL +HA SHGS+KL
Sbjct: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 569

BLAST of CmaCh13G007930 vs. ExPASy TrEMBL
Match: E5GBV5 (4-coumarate-CoA ligase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 6.7e-259
Identity = 466/569 (81.90%), Postives = 506/569 (88.93%), Query Frame = 0

Query: 1    MADRNPSLNAA-HFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
            MA RN + NAA H +DLRSGFCP TKIF SLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 487  MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 546

Query: 61   SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
             N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 547  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 606

Query: 121  LSLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMM 180
            LSLGV++SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L+ GTV+IDSP FLSM+
Sbjct: 607  LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 666

Query: 181  TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
            TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 667  TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 726

Query: 241  TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
             +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 727  MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 786

Query: 301  LVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
            LVVA+AKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 787  LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 846

Query: 361  RSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADAT 420
            R+M PEE SNTKSVGRLS +MEA IVDPASGEAL P H+GELWLRGP IMKGYVGD  AT
Sbjct: 847  RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 906

Query: 421  TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
            TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 907  TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 966

Query: 481  VIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
            VIPYPDE+AGEIPMAYVVRKPGSN++EAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 967  VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 1026

Query: 541  IDAIPKSPAGKILRRELTQHARSHGSNKL 558
            IDAIPKSPAGKILRREL +HA SHGS+KL
Sbjct: 1027 IDAIPKSPAGKILRRELAKHALSHGSSKL 1055

BLAST of CmaCh13G007930 vs. NCBI nr
Match: XP_023000795.1 (4-coumarate--CoA ligase-like 9 [Cucurbita maxima])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180
           SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of CmaCh13G007930 vs. NCBI nr
Match: XP_022923628.1 (4-coumarate--CoA ligase-like 9 [Cucurbita moschata])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.1e-307
Identity = 549/557 (98.56%), Postives = 553/557 (99.28%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180
           SLGV+ISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSKPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDP SGEALPPGHRGELWLRGPGIMKGYVGDA ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of CmaCh13G007930 vs. NCBI nr
Match: KAG6584140.1 (4-coumarate--CoA ligase-like 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.6e-307
Identity = 549/557 (98.56%), Postives = 552/557 (99.10%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180
           SLGV+ISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDP SGEALPPGHRGELWLRGPGIMKGYVGDA ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of CmaCh13G007930 vs. NCBI nr
Match: XP_023519270.1 (4-coumarate--CoA ligase-like 9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1063.5 bits (2749), Expect = 6.1e-307
Identity = 547/557 (98.20%), Postives = 553/557 (99.28%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD+RSGFCPQTKIFRSLRPPL+IPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180
           SLGV+ISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDG+ADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKF VTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 300

Query: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDP SGEALPPGHRGELWLRGPGIMKGYVGDA ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of CmaCh13G007930 vs. NCBI nr
Match: KAG7019731.1 (4-coumarate--CoA ligase-like 9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.0e-306
Identity = 548/557 (98.38%), Postives = 552/557 (99.10%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180
           SLGV+ISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDP SGEALPPGHRGELWLRGPGIMKGYVGDA ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of CmaCh13G007930 vs. TAIR 10
Match: AT5G63380.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 658.7 bits (1698), Expect = 4.2e-189
Identity = 352/565 (62.30%), Postives = 434/565 (76.81%), Query Frame = 0

Query: 9   NAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNA------ 68
           N +  +D  SGF  +T I+ SLRP L +PP+ QPLS  E ALSLL  S PP+ A      
Sbjct: 6   NDSRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEA 65

Query: 69  -AVLVDSNSGVHLSYAIFLRQIRTLASNLK-ALTSLSNGQVAFILAPTSLEVPVLYFALL 128
              LV+S+SG +L+Y   LR++R+LA +L+    SL++  VAFIL+P+SL++PVLY AL+
Sbjct: 66  LTYLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALM 125

Query: 129 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKL--PKLQLGTVLIDSPAFLSM 188
           S+GV++SPANPIGS+SE+SHQ+ +SEPVIAFATS T  KL    L LGTVL+DS  FLS 
Sbjct: 126 SIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSW 185

Query: 189 MTEINRSDGLA----DVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVN--SSPNPLL 248
              +NRSD  +     V+V+QSD AAIL+SSGTTGRVKGVLL+HRNLI     S    L 
Sbjct: 186 ---LNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQ 245

Query: 249 SEVNNGETEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVT 308
             VN       +V L  LPLFHVFGF+M++RAIS GETLVL+ +F+   M  AVEK++VT
Sbjct: 246 DPVNYD-----RVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVT 305

Query: 309 YIPVSPPLVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 368
            +PVSPPL+VAL KSEL  KYDL SL+ LGCGGAPLGK++ E+F QK P V+I+QGYGLT
Sbjct: 306 GMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLT 365

Query: 369 ESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGY 428
           ES+  AA + GPEE     SVGR+SENMEA IVDP++GE+LPPG  GELWLRGP IMKGY
Sbjct: 366 ESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGY 425

Query: 429 VGDADATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 488
           VG+  A+ ET+  +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN
Sbjct: 426 VGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSN 485

Query: 489 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAI 548
           P++IDAAV+P+PDEDAGEIPMA++VRKPGSN+ EAQ+IDF+AKQV PYKK+RRV+FI+AI
Sbjct: 486 PDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAI 545

Query: 549 PKSPAGKILRRELTQHARSHGSNKL 558
           PK+PAGKILRRELT+ A    ++KL
Sbjct: 546 PKNPAGKILRRELTKIAVDGNASKL 562

BLAST of CmaCh13G007930 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-127
Identity = 247/553 (44.67%), Postives = 360/553 (65.10%), Query Frame = 0

Query: 14  VDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           V+ RSGFC     F S R P+ +PP +  L VT        SS         +D+++G +
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDVTTFI-----SSQAHRGRIAFIDASTGQN 63

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVIISPANPIG 133
           L++    R + ++A  L  +  +  G V  +L+P S+  PV+  +++SLG II+  NP+ 
Sbjct: 64  LTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 123

Query: 134 SDSEISHQIRLSEPVIAFAT-------SSTASKLPKLQLGTVLIDSPAFLSMMTEINR-- 193
           + +EI+ QI+ S PV+AF T       S+ A KLP + +    +DS   +  + E+ +  
Sbjct: 124 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 183

Query: 194 -SDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNN-GETEP 253
            S      +VDQ D+A +LYSSGTTG  KGV+ SHRNLI +      + + VN  G  + 
Sbjct: 184 PSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAM------VQTIVNRFGSDDG 243

Query: 254 RQVSLCLLPLFHVFGFVMLLRA-ISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPLV 313
            Q  +C +P+FH++G        ++ G T++++ KF+  EM+SA+ K++ T +P+ PP++
Sbjct: 244 EQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPIL 303

Query: 314 VALAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 373
           VA+     ++ AKYDLSS+  + CGGAPL KEV E F +K P V+I+QGYGLTEST   A
Sbjct: 304 VAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGA 363

Query: 374 RSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADAT 433
            +   EES    + G+LS +ME  IVDP +G+ L P   GELWL+GP IMKGY  + +AT
Sbjct: 364 STDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEAT 423

Query: 434 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 493
           + TL  +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL ++PEI DAA
Sbjct: 424 SSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAA 483

Query: 494 VIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 553
           VIP+PD++ G+ PMAYVVRK GS+++E  +++F+AKQVAPYK+IR+V+F+ +IPK+P+GK
Sbjct: 484 VIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGK 543

BLAST of CmaCh13G007930 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 449.5 bits (1155), Expect = 3.9e-126
Identity = 261/554 (47.11%), Postives = 349/554 (63.00%), Query Frame = 0

Query: 14  VDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           VD +SGFC  T IF S R P+ +PP +Q L VT        +S P     V VD+ +G  
Sbjct: 21  VDRKSGFCESTSIFYSKREPMALPP-NQFLDVTSFI-----ASQPHRGKTVFVDAVTGRR 80

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVIISPANPIG 133
           LS+      +  +A  L AL  +  G V  IL+P S+  P++  +++SLG II+ ANPI 
Sbjct: 81  LSFPELWLGVERVAGCLYAL-GVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPIN 140

Query: 134 SDSEISHQIRLSEPVIAFATSSTASKL---PKLQLGTVLID-----SPAF---------L 193
           +  EIS QI  S PV+AF T    SKL       L  VL+D     S ++         L
Sbjct: 141 TSDEISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRL 200

Query: 194 SMMTEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVN 253
             M E   S+     +V+Q D+AA+LYSSGTTG  KGV+LSHRNLI         L +  
Sbjct: 201 ETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA--------LVQAY 260

Query: 254 NGETEPRQVSLCLLPLFHVFGF-VMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIP 313
                  Q ++C +P+ H+FGF       I+ G T+V++ KFD  ++LSAVE  R +Y+ 
Sbjct: 261 RARFGLEQRTICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLS 320

Query: 314 VSPPLVVALAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTE 373
           + PP+VVA+    +E+ +KYDLSSL  +  GGAPL +EV EKF +  PKV+I+QGYGLTE
Sbjct: 321 LVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTE 380

Query: 374 STAGAARSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYV 433
           STA AA     EE+    + G L+ N+E  IVDP +G  L     GELW+R P +MKGY 
Sbjct: 381 STAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYF 440

Query: 434 GDADATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNP 493
            + +AT  T+  +GWLKTGDLCY D DGF+++VDRLKELIK   YQV PAELE LL ++P
Sbjct: 441 KNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHP 500

Query: 494 EIIDAAVIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIP 548
           EI DAAVIP PD  AG+ PMAY+VRK GSN++E++++ F+AKQV+PYKKIR+V+F+ +IP
Sbjct: 501 EIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIP 559

BLAST of CmaCh13G007930 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 438.3 bits (1126), Expect = 9.0e-123
Identity = 247/554 (44.58%), Postives = 347/554 (62.64%), Query Frame = 0

Query: 14  VDLRSGFCPQTKIFRSLRPPLLIPP-LSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGV 73
           VD RSGFC     F S R PL +PP LS+ ++          SS P       +D+ +G 
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTT-------FISSQPHRGKTAFIDAATGQ 69

Query: 74  HLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVIISPANPI 133
            L+++   R +  +A  L     +  G V  IL+P S+ +PV+  +++SLG + + AN +
Sbjct: 70  CLTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTL 129

Query: 134 GSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAF------------LSMMT 193
            +  EIS QI  S P + F T   A KLP + +  VL D   +            LS M 
Sbjct: 130 NTSGEISKQIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTSAIRVVGILSEMV 189

Query: 194 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 253
           +   S      +V+Q D+A +LYSSGTTG  KGV+ SHRNL          ++   +   
Sbjct: 190 KKEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNL-------TAHVARFISDNL 249

Query: 254 EPRQVSLCLLPLFHVFGFV-MLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 313
           +   + +C +P+FH +G +   +  ++ G T+V++++F   +M+ AVEK R T + ++PP
Sbjct: 250 KRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPP 309

Query: 314 LVVALAKSE--LVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAG 373
           ++VA+      + AKYDLSSL+ + CGGAPL KEV E F +K P V+I+QGY LTES  G
Sbjct: 310 VLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGG 369

Query: 374 AARSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDAD 433
            A +   EES    + G L+ ++EA IVDP +G  +     GELWL+GP I KGY  + +
Sbjct: 370 GAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQE 429

Query: 434 ATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIID 493
           AT ET++ +GWLKTGDLCY D DGFL++VDRLKELIKYK YQVPPAELE LL ++P+I+D
Sbjct: 430 ATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILD 489

Query: 494 AAVIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPA 552
           AAVIP+PD++AG+ PMAYVVRK  SN++E QVIDFI+KQVAPYKKIR+VSFI++IPK+ +
Sbjct: 490 AAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTAS 548

BLAST of CmaCh13G007930 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 419.9 bits (1078), Expect = 3.3e-117
Identity = 238/560 (42.50%), Postives = 345/560 (61.61%), Query Frame = 0

Query: 7   SLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLV 66
           S  ++  +D R+GFC     F S R PL +P   + L +T        SS         +
Sbjct: 4   SQRSSSLIDPRNGFCTSNSTFYSKRKPLALPS-KESLDITTFI-----SSQTYRGKTAFI 63

Query: 67  DSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVII 126
           D+ +   +S++     +  +A  L     +  G V  +L+P ++ +P++  +++SLG ++
Sbjct: 64  DAATDHRISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVL 123

Query: 127 SPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVL------IDSPAFLSM-- 186
           + ANP+ + SEI  QI  S P +AF T   A K+    +  VL      +  P  L +  
Sbjct: 124 TTANPLNTASEILRQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVVG 183

Query: 187 -MTEINRSDGLADV---KVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSE 246
            +TE+ + +        +V + D+A +LYSSGTTGR KGV  SH NLI        +   
Sbjct: 184 NLTEMMKKEPSGQAVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIA------HVARY 243

Query: 247 VNNGETEPRQVSLCLLPLFHVFGFV-MLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTY 306
           +     +P+Q  +C +PLFH FG +  +L  ++ G T+V++ +FD  EM++AVEK+R T 
Sbjct: 244 IAEPFEQPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATT 303

Query: 307 IPVSPPLVVALAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGL 366
           + + PP++V +     +++ KYD+S L+ + CGGAPL KEV + F +K P V++ QGY L
Sbjct: 304 LILVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYAL 363

Query: 367 TESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKG 426
           TES    A     EES    +VG LS  +EA IVDP +G+ +     GELWL+GP I KG
Sbjct: 364 TESNGAGASIESVEESRRYGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKG 423

Query: 427 YVGDADATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQS 486
           Y  + +   E +  +GWLKTGDLCY D+DGFL+IVDRLKELIKYK YQVPPAELE LL +
Sbjct: 424 YFRNEE---EIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLN 483

Query: 487 NPEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDA 546
           +P+I+DAAVIP+PD++AG+ PMAYV RKP SN+ E +VIDFI+KQVAPYKKIR+V+FID+
Sbjct: 484 HPDILDAAVIPFPDKEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDS 543

Query: 547 IPKSPAGKILRRELTQHARS 552
           IPK+P+GK LR++L + A S
Sbjct: 544 IPKTPSGKTLRKDLIKFAIS 548