CmaCh13G007680 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G007680
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionProtein kinase domain-containing protein
LocationCma_Chr13: 6634688 .. 6638550 (-)
RNA-Seq ExpressionCmaCh13G007680
SyntenyCmaCh13G007680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAGGATTAACCCTGGATTGGTTATTAAAATCATTGACCGAGTTTGGGAAAAGAGATTCTCTCTAAATCTACGGTTTCTTCTCCGCAACGTGGACGGTTAGATTTCCTCTCATCCTCCACATGCTTCGTTGTTAGGTTAAAATTAGGGTTGCGTTTCGATTCCATTCCACAAATTTCTTTCTGCGGAAGAATATTTCTTCTCATCTCCCTCTTCAAAGCCCCCGGAAATCTGGTTTTTCAATTCTCTTCTCCTTTTCAAGGCTCTTCTTTTTTCTGCGAGATCATGGAGATGGAGCGCATGATCGAGTTTCCTCACACACATCTTGATCGCAGGCCCCGAAAGAGGGCTCGCTTGGGCTGGGACGTTGTCCCCGAGGCCGCTAAGGTATAATCTCCGATCCTCTCTTTCAGATCTGATTTTTATGTTGATTTTTGTTTTGTAGAGCTACATCTTCTGGGTTTTTCTTTGATTAGGTATGTTTGTGTCGCACACCGGGGTATAATCTTTTTGTTATTAGATTTACCTTGGTTGATTTACGTTTTTTTTTAAATTTTTTGTGATTCAGTATTCTCGATAGGTTCTATGAGTCGAGGTTATTTTCTTGAAGTTTATTTACTGATCTGTTGTGTTAACGTATTTTTATTTTATTTGTAAGCTCAAAAGAAGATCAGGTAAGTATTGTTCTTTATTCAGATGGATGGTTAACTCCGAATTGTCTCTTCATATTGATTATCTGTTATTGATCCATTCCACAATTTGTTTTACTTAGGGGTCTCCCTTTATTTTATACCTCGTACATGTCTGTCTACCATGCATCTGGCTTATATGTATATTTTTTTTCATTTTTCCAACAAAATCTTATAATTTCTTGTGTTATACAAAATGAAGACTTTGGGTGAGGATATTAGGTTTAGGAAGGGCAAACTTTTACATGAATGGGTGACTGTGTGTAGGCTCAGGTAGGAATTTGTTGTGGACAAGAGATTGCGAATATGCCAAGCTTTGCATCTTCAAGAGCACCTTCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCTCGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGACATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTGTAATTACACTTGACTATGATTAGACAAATTTTTATGCGTTATCAAAATTGATCAATGTAGAAATTTGCTCGTATAATTTAGTTCCTCATCTCATTTTAAATGTTTGCTATATCCTCAAATTTTGCATCCTCCCATAAATTGTATTTAAAATAATATCCATCATGATCACCCATGTTTATTTAATTTTAAACTGAACATTCAGCCATTAGTTATGGTTTTTTTTTCTTTTCATAATTATCACTTTTTTAGAATGGTGGAAGGCTGGTGAGATTTTGGGAAATATCTATTTCCTTACTCTGTCAATTCTTTTTCTTCTGTGGCAGATAAAATACACAGCAAAATGGGAGAAGGTACTTTTGGACAGGTGTTGGAATGCTGGGACAGAGAAAGGAAGGAAATGGTTGCAATAAAAATTGTGCGTGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCATGATAAAGGTGGCAACCGGTGAGTCTGTCTCTCCTTTATGCCATTGTTGTCACTCTGTGACTTTTGTCATCTTTGTGGCTATGGTTAAATTGCTCTATTCCTTGCAGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATTTGTATTGTAAGTATAAGGCTGTTTTATCATGGATTAATTTCCCCGATTCACCAAATATGGGGAGTTAAAATTTGTGGGTGAACTTAAATGGTGTCAACAGGTGTTTGAGAAGCTTGGGCCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATGTGTGTAGTATTTTATTGAACCAAGTCCTTTCTTTTCCCATTCTTATTGTCCTTTTGTGTGTCACAAAACATTAACTCCACAGATTTCTTTAAGCTAAAATGACTATTTGCATCTGCTGCATGTTCCATTTTTGTAATTTCATTTAAATGGTATGATGAGTCGGTCTCCTTTTATTTTGTGTCTTGTATCCTAATAATTTCTTTGACCTTTGAATTGCAGTCATGCATGATCTACGAATGATACATACTGACTTGAAGCCAGAGAATATACTGTTGGTCTCTGCAGATTATATGAAAGTACATGACTACAAGGTACTTATAGCTTGTTTTGGATCTTGTTTGTAATCTGTATTTCTGGTGGTTCTTATTGTGAAAGACCAATTACCAGCTAAATGAAGTTCTTGTTACTCATTATTTATGCTTGTGTCAGAATTTTTCTCGATCACCAAGGGATAGTTCCAACTTCAAAAGAGTGCCCAAGTCAAGTTTTATTAAGGTGATTGATTTTGGCAGCACCACTTATGATCGTCAAGATCAGAATTACATTGTATCAACCCGGCATTATAGAGCTCCAGAGGTTATTCTTGGTAAGGAATTTTGATCATGTTTCATAGTTCACTTCTCGATTTTTCTGATTTGTGTTAGAGTGGCATGGCTTAACCACGTTTATCTTTTAGGTATTGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGTATGTGCTTCTTTTGTCTTGTAATTTGTCTATTTCAATATATCCTTTTTTTGTCTATTCATCCAAGTGTTGTATTGTGTAATGTAATTTTCAATACAAAATATTTTTTAGGGTGAAGCACTGTTTCAAACTCATGAGAATTTGGAGCACTTGGCAATGATGGAGCGGGTGCTTGGTCCACTGCCGCAAGAGATGCTGAAAAGAGTAGAGTGAGTTATTTCTCATCTTTTATTTTCTTGGTGTTGAGTCGTCAAGAATCAAAATTCTGATTTTTTTGTTTTTTTGAATGTTTCCTTAGTCGGCATGCTGAAAAATACGTAAGAAGAGGTAGATTAGATTGGCCGGAAGGTGCTACTTCGAGGGATAGTATCAAGGCCGTTCAAAAGCTGCTCCGTTTACCGGTTTGTATTTTTATTAGTGTCTTATTTGTTTGATTGGACTGAACCTTTTTGTCGCCACTTCACACTAATTATCTTTTGAAATCGAATGCAGAACCTGATAATGCAGCATGTGGACCATTCGGCTGGGGAATTAATTCATCTCGTGCAAGGGTTGCTTCGATATGATCCCACACATAGACTAACGGCTCGTGAAGCCCTAAGGCACCCGTTCTTTACAAGGGATCCCTTGAGGAGATAGTAGCTGTGCTGTGTTTAATTAACGAACTGGTATTTGGTTTTATTTTTCAAACACCCAACGTCTTCATGCAACTGTACAGCTGCGTGTCTGTTGATCGATGATGGTGATGTGCCTTTGTATGGTGATGTGCCTTTGTATGATGATGTGCCTTTGTATGGCTCTAAGAGACCGAGTATTTTGCCGTGATGTTGTAAATAGATACGCTGACTCCTATCAGAGGCTTGCAATTCAAGCACTATTTGTGCTTAATTGCAGTTCAGTTGATTACTAATGTTTTAACTTGTTTAGAAGAATATACACTAACATTTTATAACATTTGCCGTCCATTCCTTCAAGATTATCATAAAAGACTATTGGAATTGTGATACATAATTTTATTAATTTTTGTGAGTTACTATAGTGAAAATGTATTCCAAAAACGTTAATAGAATCTTAAACTCCTTGAATTGTTGATTTTGAATAAAAAGTATCGTTCATAA

mRNA sequence

ATGGTTAGGATTAACCCTGGATTGGTTATTAAAATCATTGACCGAGTTTGGGAAAAGAGATTCTCTCTAAATCTACGAATATTTCTTCTCATCTCCCTCTTCAAAGCCCCCGGAAATCTGGTTTTTCAATTCTCTTCTCCTTTTCAAGGCTCTTCTTTTTTCTGCGAGATCATGGAGATGGAGCGCATGATCGAGTTTCCTCACACACATCTTGATCGCAGGCCCCGAAAGAGGGCTCGCTTGGGCTGGGACGTTGTCCCCGAGGCCGCTAAGGCTCAGGTAGGAATTTGTTGTGGACAAGAGATTGCGAATATGCCAAGCTTTGCATCTTCAAGAGCACCTTCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCTCGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGACATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTATAAAATACACAGCAAAATGGGAGAAGGTACTTTTGGACAGGTGTTGGAATGCTGGGACAGAGAAAGGAAGGAAATGGTTGCAATAAAAATTGTGCGTGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCATGATAAAGGTGGCAACCGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATTTGTATTGTGTTTGAGAAGCTTGGGCCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATTCATGCATGATCTACGAATGATACATACTGACTTGAAGCCAGAGAATATACTGTTGGTCTCTGCAGATTATATGAAAGTACATGACTACAAGAATTTTTCTCGATCACCAAGGGATAGTTCCAACTTCAAAAGAGTGCCCAAGTCAAGTTTTATTAAGGTGATTGATTTTGGCAGCACCACTTATGATCGTCAAGATCAGAATTACATTGTATCAACCCGGCATTATAGAGCTCCAGAGGTTATTCTTGGTATTGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGGTGAAGCACTGTTTCAAACTCATGAGAATTTGGAGCACTTGGCAATGATGGAGCGGGTGCTTGGTCCACTGCCGCAAGAGATGCTGAAAAGAGTAGATCGGCATGCTGAAAAATACGTAAGAAGAGGTAGATTAGATTGGCCGGAAGGTGCTACTTCGAGGGATAGTATCAAGGCCGTTCAAAAGCTGCTCCGTTTACCGAACCTGATAATGCAGCATGTGGACCATTCGGCTGGGGAATTAATTCATCTCGTGCAAGGGTTGCTTCGATATGATCCCACACATAGACTAACGGCTCGTGAAGCCCTAAGGCACCCGTTCTTTACAAGGGATCCCTTGAGGAGATAGTAGCTGTGCTGTGTTTAATTAACGAACTGGTATTTGGTTTTATTTTTCAAACACCCAACGTCTTCATGCAACTGTACAGCTGCGTGTCTGTTGATCGATGATGGTGATGTGCCTTTGTATGGTGATGTGCCTTTGTATGATGATGTGCCTTTGTATGGCTCTAAGAGACCGAGTATTTTGCCGTGATGTTGTAAATAGATACGCTGACTCCTATCAGAGGCTTGCAATTCAAGCACTATTTGTGCTTAATTGCAGTTCAGTTGATTACTAATGTTTTAACTTGTTTAGAAGAATATACACTAACATTTTATAACATTTGCCGTCCATTCCTTCAAGATTATCATAAAAGACTATTGGAATTGTGATACATAATTTTATTAATTTTTGTGAGTTACTATAGTGAAAATGTATTCCAAAAACGTTAATAGAATCTTAAACTCCTTGAATTGTTGATTTTGAATAAAAAGTATCGTTCATAA

Coding sequence (CDS)

ATGGTTAGGATTAACCCTGGATTGGTTATTAAAATCATTGACCGAGTTTGGGAAAAGAGATTCTCTCTAAATCTACGAATATTTCTTCTCATCTCCCTCTTCAAAGCCCCCGGAAATCTGGTTTTTCAATTCTCTTCTCCTTTTCAAGGCTCTTCTTTTTTCTGCGAGATCATGGAGATGGAGCGCATGATCGAGTTTCCTCACACACATCTTGATCGCAGGCCCCGAAAGAGGGCTCGCTTGGGCTGGGACGTTGTCCCCGAGGCCGCTAAGGCTCAGGTAGGAATTTGTTGTGGACAAGAGATTGCGAATATGCCAAGCTTTGCATCTTCAAGAGCACCTTCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCTCGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGACATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTATAAAATACACAGCAAAATGGGAGAAGGTACTTTTGGACAGGTGTTGGAATGCTGGGACAGAGAAAGGAAGGAAATGGTTGCAATAAAAATTGTGCGTGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCATGATAAAGGTGGCAACCGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATTTGTATTGTGTTTGAGAAGCTTGGGCCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATTCATGCATGATCTACGAATGATACATACTGACTTGAAGCCAGAGAATATACTGTTGGTCTCTGCAGATTATATGAAAGTACATGACTACAAGAATTTTTCTCGATCACCAAGGGATAGTTCCAACTTCAAAAGAGTGCCCAAGTCAAGTTTTATTAAGGTGATTGATTTTGGCAGCACCACTTATGATCGTCAAGATCAGAATTACATTGTATCAACCCGGCATTATAGAGCTCCAGAGGTTATTCTTGGTATTGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGGTGAAGCACTGTTTCAAACTCATGAGAATTTGGAGCACTTGGCAATGATGGAGCGGGTGCTTGGTCCACTGCCGCAAGAGATGCTGAAAAGAGTAGATCGGCATGCTGAAAAATACGTAAGAAGAGGTAGATTAGATTGGCCGGAAGGTGCTACTTCGAGGGATAGTATCAAGGCCGTTCAAAAGCTGCTCCGTTTACCGAACCTGATAATGCAGCATGTGGACCATTCGGCTGGGGAATTAATTCATCTCGTGCAAGGGTTGCTTCGATATGATCCCACACATAGACTAACGGCTCGTGAAGCCCTAAGGCACCCGTTCTTTACAAGGGATCCCTTGAGGAGATAG

Protein sequence

MVRINPGLVIKIIDRVWEKRFSLNLRIFLLISLFKAPGNLVFQFSSPFQGSSFFCEIMEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR
Homology
BLAST of CmaCh13G007680 vs. ExPASy Swiss-Prot
Match: P51567 (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=1 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 1.4e-217
Identity = 361/428 (84.35%), Postives = 400/428 (93.46%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A KAQVG+ CGQEI N+ SFASS APSD+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SS+ L VKGVARNGSPPWREDDKDGHY+F LG++LT RYKI+SKMGEGTFGQVLECWDRE
Sbjct: 61  SSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           RKEMVA+KIVRG++KYR+AAMIEIE+LQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           G SLYDFLRKNNYRSFPIDLVR+IG QLLECVAFMHDLRMIHTDLKPENILLVS+DY+K+
Sbjct: 181 GSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKI 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
            +YK  SR  RD   +KRVPKSS IKVIDFGSTTY+RQDQ YIVSTRHYRAPEVILG+GW
Sbjct: 241 PEYKG-SRLQRDVC-YKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           S+PCD+WSVGCI+VELC+GEALFQTHENLEHLAMMERVLGP PQ+MLK+VDRH+EKYVRR
Sbjct: 301 SYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWP+GATSRDS+KAV KL RL NLIMQHVDHSAGELI++VQGLLR+DP+ R+TAREA
Sbjct: 361 GRLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAREA 420

Query: 478 LRHPFFTR 486
           LRHPFF R
Sbjct: 421 LRHPFFAR 425

BLAST of CmaCh13G007680 vs. ExPASy Swiss-Prot
Match: P51566 (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=2 SV=2)

HSP 1 Score: 641.0 bits (1652), Expect = 1.1e-182
Identity = 309/437 (70.71%), Postives = 358/437 (81.92%), Query Frame = 0

Query: 51  SSFFCEIMEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFA 110
           +S    I+E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FA
Sbjct: 10  ASSIAMILETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFA 69

Query: 111 SSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQ 170
           S   P+    N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQ
Sbjct: 70  SGLVPNFVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQ 129

Query: 171 VLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNH 230
           VLEC+D + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNH
Sbjct: 130 VLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNH 189

Query: 231 ICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILL 290
           ICIVFEKLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILL
Sbjct: 190 ICIVFEKLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 249

Query: 291 VSADYMKVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAP 350
           VS++Y+K+ DYK  SR  +D S FK +PKSS IK+IDFGSTT++ QD NYIVSTRHYRAP
Sbjct: 250 VSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAP 309

Query: 351 EVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDR 410
           EVILG+GW++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR
Sbjct: 310 EVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADR 369

Query: 411 HAEKYVRRG-RLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDP 470
            +EKY RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAG+LI L+QGLLRYDP
Sbjct: 370 RSEKYFRRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDP 429

Query: 471 THRLTAREALRHPFFTR 486
           T R  AREAL HPFFTR
Sbjct: 430 TERFKAREALNHPFFTR 445

BLAST of CmaCh13G007680 vs. ExPASy Swiss-Prot
Match: P51568 (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=1 SV=2)

HSP 1 Score: 507.7 bits (1306), Expect = 1.5e-142
Identity = 241/388 (62.11%), Postives = 306/388 (78.87%), Query Frame = 0

Query: 100 QEIANMPSFASSRAPSDHSSNPLFVKGVARNG---SPPWREDDKDGHYMFALGENLTSRY 159
           + +   P      AP++  +    +KG   +G   SPP R+DD+DGHY+F+L +NLT RY
Sbjct: 12  ERVRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDGHYVFSLRDNLTPRY 71

Query: 160 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 219
           KI SKMGEGTFG+VLECWDR+ KE VAIKI+R I+KYRDAAMIEI+VLQ+L K DKG  R
Sbjct: 72  KILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTR 131

Query: 220 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 279
           CVQ++NWFDYRNHICIVFEKLGPSL+DFL++N Y +FP+ LVRD G QLLE VA+MH+L+
Sbjct: 132 CVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFGCQLLESVAYMHELQ 191

Query: 280 MIHTDLKPENILLVSADYMKVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQD 339
           ++HTDLKPENILLVS++ +K+ D K   RS  + ++F+ +PKSS IK+IDFGST  D + 
Sbjct: 192 LVHTDLKPENILLVSSENVKLPDNK---RSAANETHFRCLPKSSAIKLIDFGSTVCDNRI 251

Query: 340 QNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 399
            + IV TRHYR+PEVILG+GWS+ CD+WS+GCIL ELC+GEALFQTH+NLEHLAMMER L
Sbjct: 252 HHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMMERAL 311

Query: 400 GPLPQEMLKRVDRHAEKYVRRG-RLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGE 459
           GPLP+ M ++  R AEKY RRG RL+WPEGA SR+SI+AV++L RL +++ +HVD++   
Sbjct: 312 GPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLKDMVSKHVDNTRSR 371

Query: 460 LIHLVQGLLRYDPTHRLTAREALRHPFF 484
              L+ GLL YDP+ RLTA EAL HPFF
Sbjct: 372 FADLLYGLLAYDPSERLTANEALDHPFF 396

BLAST of CmaCh13G007680 vs. ExPASy Swiss-Prot
Match: P49760 (Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 7.8e-91
Identity = 175/377 (46.42%), Postives = 245/377 (64.99%), Query Frame = 0

Query: 111 SRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQV 170
           SR+ S HSS     K V         EDD +GH ++ +G+ L  RY+I S +GEGTFG+V
Sbjct: 129 SRSSSQHSSRR--AKSV---------EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRV 188

Query: 171 LECWDRER-KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGG-NRCVQIRNWFDYRN 230
           ++C D  R    VA+KI++ + KY++AA +EI VL+++ + D    N CVQ+ +WFDY  
Sbjct: 189 VQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHG 248

Query: 231 HICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENIL 290
           H+CI FE LG S +DFL+ NNY  +PI  VR +  QL + V F+HD ++ HTDLKPENIL
Sbjct: 249 HMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENIL 308

Query: 291 LVSADYMKVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRA 350
            V++DY   ++ +      RD    +R  KS+ ++V+DFGS T+D +  + IVSTRHYRA
Sbjct: 309 FVNSDYELTYNLE----KKRD----ERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRA 368

Query: 351 PEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVD 410
           PEVIL +GWS PCD+WS+GCI+ E   G  LFQTH+N EHLAMMER+LGP+P  M+++  
Sbjct: 369 PEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTR 428

Query: 411 RHAEKYVRRGRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDP 470
           +  +KY  RGRLDW E  ++   ++   K LR   L  +  +H   +L  L++ +L Y+P
Sbjct: 429 K--QKYFYRGRLDWDENTSAGRYVRENCKPLR-RYLTSEAEEHH--QLFDLIESMLEYEP 481

Query: 471 THRLTAREALRHPFFTR 486
             RLT  EAL+HPFF R
Sbjct: 489 AKRLTLGEALQHPFFAR 481

BLAST of CmaCh13G007680 vs. ExPASy Swiss-Prot
Match: O35491 (Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2)

HSP 1 Score: 335.1 bits (858), Expect = 1.3e-90
Identity = 167/349 (47.85%), Postives = 235/349 (67.34%), Query Frame = 0

Query: 137 EDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRER-KEMVAIKIVRGIRKYRD 196
           EDD +GH ++ +G+ L  RY+I S +GEGTFG+V++C D  R    VA+KI++ + KY++
Sbjct: 143 EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGTQVALKIIKNVEKYKE 202

Query: 197 AAMIEIEVLQQLGKHDKGG-NRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFP 256
           AA +EI VL+++ + D    N CVQ+ +WFDY  H+CI FE LG S +DFL+ NNY  +P
Sbjct: 203 AARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP 262

Query: 257 IDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKVHDYKNFSRSPRDSSNFK 316
           I  VR +  QL + V F+HD ++ HTDLKPENIL V++DY   ++ +      RD    +
Sbjct: 263 IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLE----KKRD----E 322

Query: 317 RVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELC 376
           R  KS+ ++V+DFGS T+D +  + IVSTRHYRAPEVIL +GWS PCD+WS+GCI+ E  
Sbjct: 323 RSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYY 382

Query: 377 SGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKA 436
            G  LFQTH+N EHLAMMER+LGP+P  M+++  +  +KY  RGRLDW E  ++   ++ 
Sbjct: 383 VGFTLFQTHDNREHLAMMERILGPVPSRMIRKTRK--QKYFYRGRLDWDENTSAGRYVRE 442

Query: 437 VQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREALRHPFF 484
             K LR   L  +  DH   +L  L++ +L Y+P  RLT  EAL+HPFF
Sbjct: 443 NCKPLR-RYLTSEAEDHH--QLFDLIENMLEYEPAKRLTLGEALQHPFF 478

BLAST of CmaCh13G007680 vs. ExPASy TrEMBL
Match: A0A6J1KKP8 (serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495446 PE=4 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.3e-258
Identity = 433/433 (100.00%), Postives = 433/433 (100.00%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 478 LRHPFFTRDPLRR 491
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. ExPASy TrEMBL
Match: A0A6J1EDB4 (serine/threonine-protein kinase AFC2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431493 PE=4 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.5e-257
Identity = 431/433 (99.54%), Postives = 432/433 (99.77%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKNFSRSPRDSSNFKRVPKSS IKVIDFGSTTYDR+DQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDREDQNYIVSTRHYRAPEVILGIGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 478 LRHPFFTRDPLRR 491
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. ExPASy TrEMBL
Match: A0A5A7UP87 (Serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004120 PE=4 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.5e-251
Identity = 418/433 (96.54%), Postives = 426/433 (98.38%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           +KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 KKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDI RQLLECVAFMHDLRMIHTDLKPENILLVS DY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLRMIHTDLKPENILLVSGDYVKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKN SRSPRDSSNFKRVPKSS IKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQ+MLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQDMLKKVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPAHRLTAREA 420

Query: 478 LRHPFFTRDPLRR 491
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. ExPASy TrEMBL
Match: A0A1S3AXH4 (serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483972 PE=4 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.5e-251
Identity = 418/433 (96.54%), Postives = 426/433 (98.38%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           +KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 KKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDI RQLLECVAFMHDLRMIHTDLKPENILLVS DY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLRMIHTDLKPENILLVSGDYVKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKN SRSPRDSSNFKRVPKSS IKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQ+MLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQDMLKKVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPAHRLTAREA 420

Query: 478 LRHPFFTRDPLRR 491
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. ExPASy TrEMBL
Match: A0A6J1C5J1 (serine/threonine-protein kinase AFC2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008590 PE=4 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.4e-249
Identity = 417/433 (96.30%), Postives = 425/433 (98.15%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY+IHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYRIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVS DY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSVDYVKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKN SRSPRDS+NFKRVPKSS IKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSTNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKKVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGELI LVQGLLRY+P  RLTAR+A
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLPRLPNLIMQHVDHSAGELIQLVQGLLRYNPVDRLTARQA 420

Query: 478 LRHPFFTRDPLRR 491
           L+HPFFTRDPLRR
Sbjct: 421 LKHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. NCBI nr
Match: XP_023001260.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 901.7 bits (2329), Expect = 2.7e-258
Identity = 433/433 (100.00%), Postives = 433/433 (100.00%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 478 LRHPFFTRDPLRR 491
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. NCBI nr
Match: XP_023519374.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 898.7 bits (2321), Expect = 2.3e-257
Identity = 432/433 (99.77%), Postives = 432/433 (99.77%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKNFSRSPRDSSNFKRVPKSS IKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 478 LRHPFFTRDPLRR 491
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. NCBI nr
Match: XP_022923920.1 (serine/threonine-protein kinase AFC2 isoform X1 [Cucurbita moschata] >KAG6584116.1 Serine/threonine-protein kinase AFC2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019705.1 Serine/threonine-protein kinase AFC2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 897.5 bits (2318), Expect = 5.1e-257
Identity = 431/433 (99.54%), Postives = 432/433 (99.77%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKNFSRSPRDSSNFKRVPKSS IKVIDFGSTTYDR+DQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDREDQNYIVSTRHYRAPEVILGIGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 478 LRHPFFTRDPLRR 491
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. NCBI nr
Match: XP_038894261.1 (serine/threonine-protein kinase AFC2 isoform X1 [Benincasa hispida])

HSP 1 Score: 879.4 bits (2271), Expect = 1.4e-251
Identity = 420/433 (97.00%), Postives = 427/433 (98.61%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           +KE VAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 KKETVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVS+DY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKN SRSPRDSSNFKRVP+SS IKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPRSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPMHRLTAREA 420

Query: 478 LRHPFFTRDPLRR 491
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. NCBI nr
Match: XP_008439081.1 (PREDICTED: serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo] >KAA0055279.1 serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo var. makuwa] >TYJ99202.1 serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 877.1 bits (2265), Expect = 7.1e-251
Identity = 418/433 (96.54%), Postives = 426/433 (98.38%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           +KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 KKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           GPSLYDFLRKNNYRSFPIDLVRDI RQLLECVAFMHDLRMIHTDLKPENILLVS DY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLRMIHTDLKPENILLVSGDYVKV 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
           HDYKN SRSPRDSSNFKRVPKSS IKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQ+MLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQDMLKKVDRHAEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWPEGATSRDSIKAVQKL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPAHRLTAREA 420

Query: 478 LRHPFFTRDPLRR 491
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of CmaCh13G007680 vs. TAIR 10
Match: AT4G24740.1 (FUS3-complementing gene 2 )

HSP 1 Score: 756.9 bits (1953), Expect = 1.0e-218
Identity = 361/428 (84.35%), Postives = 400/428 (93.46%), Query Frame = 0

Query: 58  MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 117
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A KAQVG+ CGQEI N+ SFASS APSD+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 118 SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 177
           SS+ L VKGVARNGSPPWREDDKDGHY+F LG++LT RYKI+SKMGEGTFGQVLECWDRE
Sbjct: 61  SSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRE 120

Query: 178 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 237
           RKEMVA+KIVRG++KYR+AAMIEIE+LQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 238 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 297
           G SLYDFLRKNNYRSFPIDLVR+IG QLLECVAFMHDLRMIHTDLKPENILLVS+DY+K+
Sbjct: 181 GSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKI 240

Query: 298 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 357
            +YK  SR  RD   +KRVPKSS IKVIDFGSTTY+RQDQ YIVSTRHYRAPEVILG+GW
Sbjct: 241 PEYKG-SRLQRDVC-YKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGW 300

Query: 358 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 417
           S+PCD+WSVGCI+VELC+GEALFQTHENLEHLAMMERVLGP PQ+MLK+VDRH+EKYVRR
Sbjct: 301 SYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRR 360

Query: 418 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 477
           GRLDWP+GATSRDS+KAV KL RL NLIMQHVDHSAGELI++VQGLLR+DP+ R+TAREA
Sbjct: 361 GRLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAREA 420

Query: 478 LRHPFFTR 486
           LRHPFF R
Sbjct: 421 LRHPFFAR 425

BLAST of CmaCh13G007680 vs. TAIR 10
Match: AT3G53570.3 (FUS3-complementing gene 1 )

HSP 1 Score: 641.0 bits (1652), Expect = 8.0e-184
Identity = 308/431 (71.46%), Postives = 356/431 (82.60%), Query Frame = 0

Query: 57  IMEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFASSRAPS 116
           I+E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FAS   P+
Sbjct: 2   ILETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPN 61

Query: 117 DHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWD 176
               N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D
Sbjct: 62  FVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFD 121

Query: 177 RERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFE 236
            + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFE
Sbjct: 122 NKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFE 181

Query: 237 KLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYM 296
           KLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILLVS++Y+
Sbjct: 182 KLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYI 241

Query: 297 KVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGI 356
           K+ DYK  SR  +D S FK +PKSS IK+IDFGSTT++ QD NYIVSTRHYRAPEVILG+
Sbjct: 242 KIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGV 301

Query: 357 GWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYV 416
           GW++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY 
Sbjct: 302 GWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYF 361

Query: 417 RRG-RLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTA 476
           RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAG+LI L+QGLLRYDPT R  A
Sbjct: 362 RRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKA 421

Query: 477 REALRHPFFTR 486
           REAL HPFFTR
Sbjct: 422 REALNHPFFTR 431

BLAST of CmaCh13G007680 vs. TAIR 10
Match: AT3G53570.1 (FUS3-complementing gene 1 )

HSP 1 Score: 641.0 bits (1652), Expect = 8.0e-184
Identity = 309/437 (70.71%), Postives = 358/437 (81.92%), Query Frame = 0

Query: 51  SSFFCEIMEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFA 110
           +S    I+E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FA
Sbjct: 10  ASSIAMILETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFA 69

Query: 111 SSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQ 170
           S   P+    N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQ
Sbjct: 70  SGLVPNFVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQ 129

Query: 171 VLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNH 230
           VLEC+D + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNH
Sbjct: 130 VLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNH 189

Query: 231 ICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILL 290
           ICIVFEKLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILL
Sbjct: 190 ICIVFEKLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 249

Query: 291 VSADYMKVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAP 350
           VS++Y+K+ DYK  SR  +D S FK +PKSS IK+IDFGSTT++ QD NYIVSTRHYRAP
Sbjct: 250 VSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAP 309

Query: 351 EVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDR 410
           EVILG+GW++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR
Sbjct: 310 EVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADR 369

Query: 411 HAEKYVRRG-RLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDP 470
            +EKY RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAG+LI L+QGLLRYDP
Sbjct: 370 RSEKYFRRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDP 429

Query: 471 THRLTAREALRHPFFTR 486
           T R  AREAL HPFFTR
Sbjct: 430 TERFKAREALNHPFFTR 445

BLAST of CmaCh13G007680 vs. TAIR 10
Match: AT3G53570.2 (FUS3-complementing gene 1 )

HSP 1 Score: 641.0 bits (1652), Expect = 8.0e-184
Identity = 309/437 (70.71%), Postives = 358/437 (81.92%), Query Frame = 0

Query: 51  SSFFCEIMEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFA 110
           +S    I+E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FA
Sbjct: 10  ASSIAMILETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFA 69

Query: 111 SSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQ 170
           S   P+    N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQ
Sbjct: 70  SGLVPNFVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQ 129

Query: 171 VLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNH 230
           VLEC+D + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNH
Sbjct: 130 VLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNH 189

Query: 231 ICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILL 290
           ICIVFEKLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILL
Sbjct: 190 ICIVFEKLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 249

Query: 291 VSADYMKVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAP 350
           VS++Y+K+ DYK  SR  +D S FK +PKSS IK+IDFGSTT++ QD NYIVSTRHYRAP
Sbjct: 250 VSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAP 309

Query: 351 EVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDR 410
           EVILG+GW++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR
Sbjct: 310 EVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADR 369

Query: 411 HAEKYVRRG-RLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDP 470
            +EKY RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAG+LI L+QGLLRYDP
Sbjct: 370 RSEKYFRRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDP 429

Query: 471 THRLTAREALRHPFFTR 486
           T R  AREAL HPFFTR
Sbjct: 430 TERFKAREALNHPFFTR 445

BLAST of CmaCh13G007680 vs. TAIR 10
Match: AT3G53570.4 (FUS3-complementing gene 1 )

HSP 1 Score: 641.0 bits (1652), Expect = 8.0e-184
Identity = 309/437 (70.71%), Postives = 358/437 (81.92%), Query Frame = 0

Query: 51  SSFFCEIMEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFA 110
           +S    I+E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FA
Sbjct: 10  ASSIAMILETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFA 69

Query: 111 SSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQ 170
           S   P+    N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQ
Sbjct: 70  SGLVPNFVYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQ 129

Query: 171 VLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNH 230
           VLEC+D + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNH
Sbjct: 130 VLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNH 189

Query: 231 ICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILL 290
           ICIVFEKLGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILL
Sbjct: 190 ICIVFEKLGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 249

Query: 291 VSADYMKVHDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAP 350
           VS++Y+K+ DYK  SR  +D S FK +PKSS IK+IDFGSTT++ QD NYIVSTRHYRAP
Sbjct: 250 VSSEYIKIPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAP 309

Query: 351 EVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDR 410
           EVILG+GW++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR
Sbjct: 310 EVILGVGWNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADR 369

Query: 411 HAEKYVRRG-RLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDP 470
            +EKY RRG +LDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAG+LI L+QGLLRYDP
Sbjct: 370 RSEKYFRRGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDP 429

Query: 471 THRLTAREALRHPFFTR 486
           T R  AREAL HPFFTR
Sbjct: 430 TERFKAREALNHPFFTR 445

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P515671.4e-21784.35Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=... [more]
P515661.1e-18270.71Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=... [more]
P515681.5e-14262.11Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=... [more]
P497607.8e-9146.42Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1[more]
O354911.3e-9047.85Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KKP81.3e-258100.00serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1EDB42.5e-25799.54serine/threonine-protein kinase AFC2 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5A7UP873.5e-25196.54Serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3AXH43.5e-25196.54serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1C5J11.4e-24996.30serine/threonine-protein kinase AFC2 isoform X2 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_023001260.12.7e-258100.00serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima][more]
XP_023519374.12.3e-25799.77serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita pepo subsp. pepo... [more]
XP_022923920.15.1e-25799.54serine/threonine-protein kinase AFC2 isoform X1 [Cucurbita moschata] >KAG6584116... [more]
XP_038894261.11.4e-25197.00serine/threonine-protein kinase AFC2 isoform X1 [Benincasa hispida][more]
XP_008439081.17.1e-25196.54PREDICTED: serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo] >KAA00... [more]
Match NameE-valueIdentityDescription
AT4G24740.11.0e-21884.35FUS3-complementing gene 2 [more]
AT3G53570.38.0e-18471.46FUS3-complementing gene 1 [more]
AT3G53570.18.0e-18470.71FUS3-complementing gene 1 [more]
AT3G53570.28.0e-18470.71FUS3-complementing gene 1 [more]
AT3G53570.48.0e-18470.71FUS3-complementing gene 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 156..483
e-value: 2.7E-75
score: 266.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 156..483
e-value: 2.7E-49
score: 167.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 156..483
score: 37.304585
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 143..236
e-value: 1.4E-111
score: 374.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 210..483
e-value: 1.4E-111
score: 374.9
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 150..289
e-value: 4.2E-10
score: 36.8
NoneNo IPR availablePANTHERPTHR45646:SF16KINASE AFC1coord: 58..490
NoneNo IPR availablePANTHERPTHR45646SERINE/THREONINE-PROTEIN KINASE DOA-RELATEDcoord: 58..490
NoneNo IPR availableCDDcd14134PKc_CLKcoord: 143..483
e-value: 0.0
score: 558.334
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 277..289
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 143..486

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G007680.1CmaCh13G007680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006397 mRNA processing
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity