CmaCh13G005250 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh13G005250
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protease Do-like 5, chloroplastic
Location	Cma_Chr13: 5515649 .. 5518640 (-)
RNA-Seq Expression	CmaCh13G005250
Synteny	CmaCh13G005250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAACGATAAGATTGCTCAGTTGAAGATTCATTGGAAGAAAAGTGGAAATCCATGGCGTTGGGCTCGCTGGGAATTCGTCTTCTTCCAGTTTCATCTCCCCCTAATTCGTCGGAAATTTCCTTACCCTTCAGTTCGCGAAGAGCCATAGTGTTTGCCCCGACTGTTTTAATGGCTTCCCTGCTCGCTTTCCCTGTTCCCAGTTTTGCCGCTCTTCTCCAACTGCAAGACGAGGTTCCACAAGAAGAAGATAGAATCGTTGGCCTTTTCCAGGTCCCTTCTCATAATCTTAGATAATAACTCTAGAATTCTTCTGTTTCCATTGAAATTAGGTCTTCTTCATCTGTTTTGTTCTGTTGGTTCTGATTGCAATGTAGGAAACTTCACCGTCGGTTGTTTGTATAAAGAATCTTGAAATTGCTAAAAAACCCCAAAACCCTTCTGAAGAGGCCATGCTTATTGAGGATGATGAGAACGCCAAGGTCAAAGGGACTGGTTCGGGGTTTATTTGGGATAAATTTGGCCATATCGTAAGCTCTTCTCTTCTTTGATTCTTTACATTTCCTCTAGCGCCCTGCTGCTGCAAATTTTGAGCTGAAGAATGTATGCTTGTGACAGGTTACTAATTACCATGTCGTTTCTGCATTGGCTACTGATAATAGTGGATTACAGCGTTGTAAGGTTTTGTTCGTTAGTTTCTGTGTTTTGGATTGTTAAGCATTCTTAGTGGCTGATAGACATGCTAGCAGTCTCTAGTCTTGGTTTCTCAGTTTTGTTTGCTTCTGTAGGTAAATTTGGTCGATGTTAAAGGAAATGGAATCTCTAGAGATGCAAAGATTGTAGGATTTGATCCAGAGTATGATCTAGCTGTTCTTAAGGTATTGAATCAAGCTTGCTTGTATTTATCGATGGTGCTAATTTAGCTCTAGATTTGTGTATAGAGCATTCTTAGCTTCATCTCCTAGAAGCAAATATATGGATATGAGACACAAATACAAAAATTTAAGGCACAGACATGGAAAGAACACATTGATTACAATACAAACTTTTACTAGAAGGAAATTCAAAATCAACAAGTTTATTATGCATTTTATATGCATTAAAAAATGAGTTTTATGTATCAGTGAGATGGAGAACGGAGCATTCCATATAAGAGTGTGGAAACTTCTACCTAGTGGACGCGTTTTAAAACTTTGAGAGGAAGCTCTAAAGGGAAAGCCCAAAAAAAACAATATCGGTTATCGGTGGGCTTGGGCTTGGGTGGTTACATTTTCGTCCTACATATTATGCCTATTTAGTGCTCATCAAATATTTAATACATGTCTCACAAATGATACTCCTATATCGACTTTGTATAACTAGTGTCAAACACATAAAGTATGTGCTAACAAGTATTCTATATGTGTCCAATAAGTGTGTCCAAGTGTTTGGAACGTGGAAACACTACCCAAACTAAAATGTTTGTGCTTCTTAGAAACCATGATCGGCTGATTCTGAAGTTTCCATGTAACATCCAGGTGGAACTTCAAGGACATGAACTAAAGCCCATAGTCTTTGGTACCTCTCGAAGTTTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTCACAGCAGGAGTAAGGCATCAAGGATGTTACTTCCTCCATTTAATATAGGGAAACCCACTTTGAGTTTTCCATTCCAAAGCTAAAACTAATACTTCTCTCTCTATAATGAACATATTAATCATAGAAGTTTATAATATCTCAGATCTCACACGCATTTATGCTTTCTTCAAGTGTCATATCAAGTTTCGTACTGGTGTTCTTTACCAAGTACCACAGAGTCATATAGCAGATTAACTAAATAATTAAGAACCGTAAATCATTCAGGGTTTGGATAGCAGGTGATCAGCGGACTGGGTAGAGAAATTCCATCCCCAAATGGAAGGGCCATCAGGGGAGCTATTCAGACAGATGCTGCTATTAGTGCAGGTTCATGGTTCAACTTCCTGAAATAAGATTTTTCCATTTCAAACACTGTTCTGTTATCTTTTCATGCTCTTTCTTCTCCCTTTTCAAAGCTATTCTCCTTTCATCACTCATATTATAAGTGTTTATTGATTCCACTGATCTTGAATCTTGAAACTATCGAACAGTTCAATTCCCAGCTTATCTTGTTCTTTCTATTTGGTCTCTGGTTTATGCTGCCCAAGTCATCCATTATAATGCATAGAAGTTTTACAGGGAATTCAGGGGGGCCATTAGTTGACTTATACGGCCATGTAATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGTGAAGGATCTTAACTTCTTCATCTGCCTTAGTTTGTCAGTGGAAATATGAATGCATAATTTCTTTGCCTTAGTAAGTATCAAAGCTAGTTCAACTTTTTCTAGCCACTTGAAAGAACAGTCTTCACAATAGTAAAGAAAGAAAGAACGAATAAATAATGATATTTTTTGTGCTTGTGAAGTTCTAAGTTGCGGTTTCATGTTTGGTTATTCCCTACAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCAATACCAATAGACACAGTTGTACGGACTGTCCCATATCTTATTGTATATGGAACACCTTACAGTGAGAGATTTTGAACGAGTTTTACCATCTTAAGGGAACACAGCAGTCATTCAAGCAGAACCAACTCCTCTTCAAGACGATAGAACACACTGTAAATGAATTTGCTTTCTCTAGTGTATTATCCATATGCATTTCAAGATCTTGGTCTGAGCATTATTCTTCCTTCTCTCTGTTGGGTAATTCTATTATTTAAGCTACGATTGATGAAATGCAACGGGAAGAGTTTGGCGATTGTGTGTTTGTTTAAATGTCTACATTAAGCTTTTAACGTTAACACATTTGAGTTCTAAACCAAATTTAAAAAGTGACCATATTTCCACTAAAACATCAGATTGTTTTAGAACTTTTCTGTAGTAAT

mRNA sequence

ACAACGATAAGATTGCTCAGTTGAAGATTCATTGGAAGAAAAGTGGAAATCCATGGCGTTGGGCTCGCTGGGAATTCGTCTTCTTCCAGTTTCATCTCCCCCTAATTCGTCGGAAATTTCCTTACCCTTCAGTTCGCGAAGAGCCATAGTGTTTGCCCCGACTGTTTTAATGGCTTCCCTGCTCGCTTTCCCTGTTCCCAGTTTTGCCGCTCTTCTCCAACTGCAAGACGAGGTTCCACAAGAAGAAGATAGAATCGTTGGCCTTTTCCAGGAAACTTCACCGTCGGTTGTTTGTATAAAGAATCTTGAAATTGCTAAAAAACCCCAAAACCCTTCTGAAGAGGCCATGCTTATTGAGGATGATGAGAACGCCAAGGTCAAAGGGACTGGTTCGGGGTTTATTTGGGATAAATTTGGCCATATCGTTACTAATTACCATGTCGTTTCTGCATTGGCTACTGATAATAGTGGATTACAGCGTTGTAAGGTAAATTTGGTCGATGTTAAAGGAAATGGAATCTCTAGAGATGCAAAGATTGTAGGATTTGATCCAGAGTATGATCTAGCTGTTCTTAAGGTGGAACTTCAAGGACATGAACTAAAGCCCATAGTCTTTGGTACCTCTCGAAGTTTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTCACAGCAGGAGTGATCAGCGGACTGGGTAGAGAAATTCCATCCCCAAATGGAAGGGCCATCAGGGGAGCTATTCAGACAGATGCTGCTATTAGTGCAGGGAATTCAGGGGGGCCATTAGTTGACTTATACGGCCATGTAATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCAATACCAATAGACACAGTTGTACGGACTGTCCCATATCTTATTGTATATGGAACACCTTACAGTGAGAGATTTTGAACGAGTTTTACCATCTTAAGGGAACACAGCAGTCATTCAAGCAGAACCAACTCCTCTTCAAGACGATAGAACACACTGTAAATGAATTTGCTTTCTCTAGTGTATTATCCATATGCATTTCAAGATCTTGGTCTGAGCATTATTCTTCCTTCTCTCTGTTGGGTAATTCTATTATTTAAGCTACGATTGATGAAATGCAACGGGAAGAGTTTGGCGATTGTGTGTTTGTTTAAATGTCTACATTAAGCTTTTAACGTTAACACATTTGAGTTCTAAACCAAATTTAAAAAGTGACCATATTTCCACTAAAACATCAGATTGTTTTAGAACTTTTCTGTAGTAAT

Coding sequence (CDS)

ATGGCGTTGGGCTCGCTGGGAATTCGTCTTCTTCCAGTTTCATCTCCCCCTAATTCGTCGGAAATTTCCTTACCCTTCAGTTCGCGAAGAGCCATAGTGTTTGCCCCGACTGTTTTAATGGCTTCCCTGCTCGCTTTCCCTGTTCCCAGTTTTGCCGCTCTTCTCCAACTGCAAGACGAGGTTCCACAAGAAGAAGATAGAATCGTTGGCCTTTTCCAGGAAACTTCACCGTCGGTTGTTTGTATAAAGAATCTTGAAATTGCTAAAAAACCCCAAAACCCTTCTGAAGAGGCCATGCTTATTGAGGATGATGAGAACGCCAAGGTCAAAGGGACTGGTTCGGGGTTTATTTGGGATAAATTTGGCCATATCGTTACTAATTACCATGTCGTTTCTGCATTGGCTACTGATAATAGTGGATTACAGCGTTGTAAGGTAAATTTGGTCGATGTTAAAGGAAATGGAATCTCTAGAGATGCAAAGATTGTAGGATTTGATCCAGAGTATGATCTAGCTGTTCTTAAGGTGGAACTTCAAGGACATGAACTAAAGCCCATAGTCTTTGGTACCTCTCGAAGTTTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTCACAGCAGGAGTGATCAGCGGACTGGGTAGAGAAATTCCATCCCCAAATGGAAGGGCCATCAGGGGAGCTATTCAGACAGATGCTGCTATTAGTGCAGGGAATTCAGGGGGGCCATTAGTTGACTTATACGGCCATGTAATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCAATACCAATAGACACAGTTGTACGGACTGTCCCATATCTTATTGTATATGGAACACCTTACAGTGAGAGATTTTGA

Protein sequence

MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDEVPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQGHELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF

Homology

[Close]

BLAST of CmaCh13G005250 vs. ExPASy Swiss-Prot
Match: Q9SEL7 (Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 SV=3)

HSP 1 Score: 359.4 bits (921), Expect = 4.1e-98
Identity = 181/296 (61.15%), Postives = 225/296 (76.01%), Query Frame = 0


Query: 19  SSEISLPFSSRRAIVFAPTVLMASLL------AFPVPSFAALLQL---QDEVPQEEDRIV 78
           S+ + +    RR ++F  ++ + S L        P+ S  AL Q    ++E+ +EE+R V
Sbjct: 33  SNHVDVIDRRRRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNV 92

Query: 79  GLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDKFGHIVTNYH 138
            LFQ+TSPSVV I+ +E+ K     +    ++ D+EN K++GTGSGF+WDK GHIVTNYH
Sbjct: 93  NLFQKTSPSVVYIEAIELPK-----TSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYH 152

Query: 139 VVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQGHELKPIVFG 198
           V++ LATD  GLQRCKV+LVD KG   S++ KIVG DP+ DLAVLK+E +G EL P+V G
Sbjct: 153 VIAKLATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLG 212

Query: 199 TSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSG 258
           TS  LRVGQSC+AIGNP+GYE TLT GV+SGLGREIPSPNG++I  AIQTDA I++GNSG
Sbjct: 213 TSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGNSG 272

Query: 259 GPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 306
           GPL+D YGH IGVNTATFTRKG+GMSSGVNFAIPIDTVVRTVPYLIVYGT Y +RF
Sbjct: 273 GPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYRDRF 323

[Close]

BLAST of CmaCh13G005250 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.3e-47
Identity = 132/295 (44.75%), Postives = 175/295 (59.32%), Query Frame = 0


Query: 11  LPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDEV------PQE 70
           L V S P+++   L  S    + F P+  + S LA   PS A +  +   V         
Sbjct: 57  LAVKSVPSTTRRILLTSLFMNLCFNPSRYL-SALALGDPSVATVEDVSPTVFPAGPLFPT 116

Query: 71  EDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDKFGHI 130
           E RIV LF++ + SVV I   ++  +PQ      + I +       G GSG +WD  G+I
Sbjct: 117 EGRIVQLFEKNTYSVVNI--FDVTLRPQLKMTGVVEIPE-------GNGSGVVWDGQGYI 176

Query: 131 VTNYHVV-SALATDNS-GLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQGHE 190
           VTNYHV+ +AL+ + S G    +VN++   G   + + K+VG D   DLAVLKV+     
Sbjct: 177 VTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETL 236

Query: 191 LKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAA 250
           LKPI  G S SL+VGQ C AIGNPFG++ TLT GVISGL R+I S  G  I G IQTDAA
Sbjct: 237 LKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 296

Query: 251 ISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVY 298
           I+ GNSGGPL+D  G++IG+NTA FT+  TG S+GV FAIP  TV++ VP LI +
Sbjct: 297 INPGNSGGPLLDSKGNLIGINTAIFTQ--TGTSAGVGFAIPSSTVLKIVPQLIQF 339

[Close]

BLAST of CmaCh13G005250 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 189.9 bits (481), Expect = 4.3e-47
Identity = 123/280 (43.93%), Postives = 167/280 (59.64%), Query Frame = 0


Query: 20  SEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDEVPQEEDRIVGLFQETSPSV 79
           + ++L FS     +FA +  + S  AF V +         ++  +E   V LFQE +PSV
Sbjct: 85  TSVALSFS-----LFAASPAVESASAFVVST-------PKKLQTDELATVRLFQENTPSV 144

Query: 80  VCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDKFGHIVTNYHVVSALATDNS 139
           V I NL +        ++A  +  D     +G+GSGF+WDK GHIVTNYHV+        
Sbjct: 145 VYITNLAV-------RQDAFTL--DVLEVPQGSGSGFVWDKQGHIVTNYHVI-------R 204

Query: 140 GLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQGHELKPIVFGTSRSLRVGQS 199
           G    +V L D      + DAK+VGFD + D+AVL+++   ++L+PI  G S  L VGQ 
Sbjct: 205 GASDLRVTLAD----QTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQK 264

Query: 200 CYAIGNPFGYEKTLTAGVISGLGREIPS-PNGRAIRGAIQTDAAISAGNSGGPLVDLYGH 259
            +AIGNPFG + TLT GVISGL REI S   GR I+  IQTDAAI+ GNSGGPL+D  G 
Sbjct: 265 VFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGT 324

Query: 260 VIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYG 299
           +IG+NTA ++   +G SSGV F+IP+DTV   V  L+ +G
Sbjct: 325 LIGINTAIYS--PSGASSGVGFSIPVDTVGGIVDQLVRFG 330

[Close]

BLAST of CmaCh13G005250 vs. ExPASy Swiss-Prot
Match: Q2SL36 (Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=mucD PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 9.6e-31
Identity = 92/238 (38.66%), Postives = 130/238 (54.62%), Query Frame = 0


Query: 70  GLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDEN-------------------AKVK 129
           GL + TSP+VV   N+   +K  + S +      +++                    + +
Sbjct: 33  GLVERTSPAVV---NISTVRKVGDDSAQYYFGGPEQDQIPEFFRHFFGDPYRRRGPQEAQ 92

Query: 130 GTGSGFIWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYD 189
            TGSGFI  K G+I+TN HVV       +G     V L+D +       AK++G D + D
Sbjct: 93  STGSGFIVSKDGYILTNNHVV-------AGADEIFVRLMDRR----ELTAKLIGSDEKSD 152

Query: 190 LAVLKVELQGHELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNG 249
           LAVLKVE    +L  +  G S  L+VG+   AIG+PFG+E T+TAG++S  GR +P+ N 
Sbjct: 153 LAVLKVE--ADDLPVLNLGKSSELKVGEWVVAIGSPFGFEYTVTAGIVSAKGRSLPNENY 212

Query: 250 RAIRGAIQTDAAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVV 289
                 IQTD AI+ GNSGGPL +L G V+G+N+  +TR G  M  GV+FAIPID  +
Sbjct: 213 VPF---IQTDVAINPGNSGGPLFNLEGEVVGINSQIYTRSGGFM--GVSFAIPIDVAL 249

[Close]

BLAST of CmaCh13G005250 vs. ExPASy Swiss-Prot
Match: Q4KGQ4 (Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) OX=220664 GN=mucD PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.5e-28
Identity = 74/185 (40.00%), Postives = 107/185 (57.84%), Query Frame = 0


Query: 104 DENAKVKGTGSGFIWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIV 163
           D   + +  GSGFI    G+I+TN HV+       +      V L D         AK++
Sbjct: 91  DRQREAQSLGSGFIISADGYILTNNHVI-------ADADEILVRLADRS----ELKAKLI 150

Query: 164 GFDPEYDLAVLKVELQGHELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGR 223
           G DP  D+A+LK++  G +L  +  G S+ L+ GQ   AIG+PFG++ T+T G++S +GR
Sbjct: 151 GTDPRSDVALLKID--GKDLPVLKLGKSQDLKAGQWVVAIGSPFGFDHTVTQGIVSAIGR 210

Query: 224 EIPSPNGRAIRGAIQTDAAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIP 283
            +P+ N       IQTD  I+ GNSGGPL +L G V+G+N+  +TR G  M  GV+FAIP
Sbjct: 211 SLPNENYVPF---IQTDVPINPGNSGGPLFNLAGEVVGINSQIYTRSGGFM--GVSFAIP 257

Query: 284 IDTVV 289
           ID  +
Sbjct: 271 IDVAM 257

[Close]

BLAST of CmaCh13G005250 vs. ExPASy TrEMBL
Match: A0A6J1KMS0 (protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495580 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.0e-168
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 306
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of CmaCh13G005250 vs. ExPASy TrEMBL
Match: A0A6J1EKF8 (protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434145 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.2e-165
Identity = 300/305 (98.36%), Postives = 302/305 (99.02%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MALGSLGIRLLPVSSPPNSSEISLPF+SRRAIVFAPT LMASLLAFPVPSFAAL QLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           VPQEEDRIVGLFQETSPSVV IKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVEL+G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 306
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of CmaCh13G005250 vs. ExPASy TrEMBL
Match: A0A6J1EHG9 (protease Do-like 5, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434145 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.6e-148
Identity = 279/305 (91.48%), Postives = 281/305 (92.13%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MALGSLGIRLLPVSSPPNSSEISLPF+SRRAIVFAPT LMASLLAFPVPSFAAL QLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           VPQEEDRIVGLFQETSPSVV IKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHI                     VNLVDVKGNGISRDAKIVGFDPEYDLAVLKVEL+G
Sbjct: 121 FGHI---------------------VNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 284

Query: 301 YSERF 306
           YSERF
Sbjct: 301 YSERF 284

[Close]

BLAST of CmaCh13G005250 vs. ExPASy TrEMBL
Match: A0A6J1CEE6 (protease Do-like 5, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010776 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.0e-145
Identity = 263/305 (86.23%), Postives = 283/305 (92.79%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MAL SLGI LLP+ +PPNSS  SLPF+SRRA+VFAP+ LMASLLAFP+P+ AAL Q+Q +
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           VPQEEDR+V LFQ+ SPSVV IK+LE+AKKPQN SEEA+L+E DEN KVKGTGSGF+WDK
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVE-DENVKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVD KGNGI R+AKIVGFDPEYDLAVLKVEL G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDAKGNGIYREAKIVGFDPEYDLAVLKVELGG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
            ELKPIV GTSR+LRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRG IQTD
Sbjct: 181 CELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGGIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAIS+GNSGGPL+D YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 306
           YSERF
Sbjct: 301 YSERF 304

[Close]

BLAST of CmaCh13G005250 vs. ExPASy TrEMBL
Match: A0A5D3DAW0 (Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00660 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.3e-144
Identity = 263/305 (86.23%), Postives = 282/305 (92.46%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MALG LGI  LP+ +PPNSSE  LPF+SRRAI+F+PT LMASLLAFP+P+ AAL QLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           + QEEDRIV LFQETSPSVV IK+LE+AK PQN SEE  ++ +D+N KVKGTGSGF+WDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHIVTNYHVVSALATDNSG QRCKVNLVDVKGNGI ++A IVGFDPEYDLAVLKVEL+G
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCKVNLVDVKGNGIYKEANIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSR+LRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 306
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of CmaCh13G005250 vs. NCBI nr
Match: XP_023001449.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 600.9 bits (1548), Expect = 6.1e-168
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 306
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of CmaCh13G005250 vs. NCBI nr
Match: XP_023520040.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 590.5 bits (1521), Expect = 8.3e-165
Identity = 298/305 (97.70%), Postives = 302/305 (99.02%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MA GSLGIR+LPVSSPPNSSEISLPF+SRRAIVFAPT LMASLLAFPVPSFAAL QLQDE
Sbjct: 1   MASGSLGIRVLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNL+DVKGNGISRDAK+VGFDPEYDLAVLKVELQG
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLLDVKGNGISRDAKVVGFDPEYDLAVLKVELQG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 306
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of CmaCh13G005250 vs. NCBI nr
Match: XP_022927238.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 590.1 bits (1520), Expect = 1.1e-164
Identity = 300/305 (98.36%), Postives = 302/305 (99.02%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MALGSLGIRLLPVSSPPNSSEISLPF+SRRAIVFAPT LMASLLAFPVPSFAAL QLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           VPQEEDRIVGLFQETSPSVV IKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVEL+G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300

Query: 301 YSERF 306
           YSERF
Sbjct: 301 YSERF 305

[Close]

BLAST of CmaCh13G005250 vs. NCBI nr
Match: KAG6583889.1 (Protease Do-like 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 570.9 bits (1470), Expect = 6.8e-159
Identity = 291/303 (96.04%), Postives = 295/303 (97.36%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MALGSLGIRLLPVSSPPNSSEISLPF+SRRAIVFAPT LMASLLAFPVPSFAAL QLQDE
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           VPQEEDRIVGLFQETSPSVV IKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVDV+GNGISRDAK+VGFDPEYDLAVLKVELQG
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVEGNGISRDAKVVGFDPEYDLAVLKVELQG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLI   TP
Sbjct: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIAEPTP 300

Query: 301 YSE 304
             +
Sbjct: 301 LQD 303

[Close]

BLAST of CmaCh13G005250 vs. NCBI nr
Match: XP_038893976.1 (protease Do-like 5, chloroplastic [Benincasa hispida])

HSP 1 Score: 541.6 bits (1394), Expect = 4.4e-150
Identity = 273/305 (89.51%), Postives = 288/305 (94.43%), Query Frame = 0


Query: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60
           MALGSLGIRLLP+SSPPNS+E  LP +SRRAIVFAPT LMASLLAFPVP++AAL QLQD+
Sbjct: 1   MALGSLGIRLLPISSPPNSAENPLPITSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60

Query: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120
           +PQEEDRIV LFQETSPSVV IK+LE+AK PQNPS E      DENAKVKGTGSGF+WDK
Sbjct: 61  IPQEEDRIVALFQETSPSVVYIKDLEVAKNPQNPSGE------DENAKVKGTGSGFVWDK 120

Query: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQG 180
           FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGI ++AKIVGFDPEYDLAVLKVEL+G
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCKVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVELEG 180

Query: 181 HELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240
           HELKPIVFGTSR+LRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD
Sbjct: 181 HELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTD 240

Query: 241 AAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTP 300
           AAISAGNSGGPLVD YGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV+RTVPYLIVYGTP
Sbjct: 241 AAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVIRTVPYLIVYGTP 299

Query: 301 YSERF 306
           YSERF
Sbjct: 301 YSERF 299

[Close]

BLAST of CmaCh13G005250 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 359.4 bits (921), Expect = 2.9e-99
Identity = 181/296 (61.15%), Postives = 225/296 (76.01%), Query Frame = 0


Query: 19  SSEISLPFSSRRAIVFAPTVLMASLL------AFPVPSFAALLQL---QDEVPQEEDRIV 78
           S+ + +    RR ++F  ++ + S L        P+ S  AL Q    ++E+ +EE+R V
Sbjct: 33  SNHVDVIDRRRRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNV 92

Query: 79  GLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDKFGHIVTNYH 138
            LFQ+TSPSVV I+ +E+ K     +    ++ D+EN K++GTGSGF+WDK GHIVTNYH
Sbjct: 93  NLFQKTSPSVVYIEAIELPK-----TSSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYH 152

Query: 139 VVSALATDNSGLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQGHELKPIVFG 198
           V++ LATD  GLQRCKV+LVD KG   S++ KIVG DP+ DLAVLK+E +G EL P+V G
Sbjct: 153 VIAKLATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELNPVVLG 212

Query: 199 TSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSG 258
           TS  LRVGQSC+AIGNP+GYE TLT GV+SGLGREIPSPNG++I  AIQTDA I++GNSG
Sbjct: 213 TSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSISEAIQTDADINSGNSG 272

Query: 259 GPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 306
           GPL+D YGH IGVNTATFTRKG+GMSSGVNFAIPIDTVVRTVPYLIVYGT Y +RF
Sbjct: 273 GPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYRDRF 323

[Close]

BLAST of CmaCh13G005250 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 190.3 bits (482), Expect = 2.3e-48
Identity = 132/295 (44.75%), Postives = 175/295 (59.32%), Query Frame = 0


Query: 11  LPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDEV------PQE 70
           L V S P+++   L  S    + F P+  + S LA   PS A +  +   V         
Sbjct: 57  LAVKSVPSTTRRILLTSLFMNLCFNPSRYL-SALALGDPSVATVEDVSPTVFPAGPLFPT 116

Query: 71  EDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDKFGHI 130
           E RIV LF++ + SVV I   ++  +PQ      + I +       G GSG +WD  G+I
Sbjct: 117 EGRIVQLFEKNTYSVVNI--FDVTLRPQLKMTGVVEIPE-------GNGSGVVWDGQGYI 176

Query: 131 VTNYHVV-SALATDNS-GLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQGHE 190
           VTNYHV+ +AL+ + S G    +VN++   G   + + K+VG D   DLAVLKV+     
Sbjct: 177 VTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETL 236

Query: 191 LKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAA 250
           LKPI  G S SL+VGQ C AIGNPFG++ TLT GVISGL R+I S  G  I G IQTDAA
Sbjct: 237 LKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 296

Query: 251 ISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVY 298
           I+ GNSGGPL+D  G++IG+NTA FT+  TG S+GV FAIP  TV++ VP LI +
Sbjct: 297 INPGNSGGPLLDSKGNLIGINTAIFTQ--TGTSAGVGFAIPSSTVLKIVPQLIQF 339

[Close]

BLAST of CmaCh13G005250 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 189.9 bits (481), Expect = 3.0e-48
Identity = 123/280 (43.93%), Postives = 167/280 (59.64%), Query Frame = 0


Query: 20  SEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDEVPQEEDRIVGLFQETSPSV 79
           + ++L FS     +FA +  + S  AF V +         ++  +E   V LFQE +PSV
Sbjct: 85  TSVALSFS-----LFAASPAVESASAFVVST-------PKKLQTDELATVRLFQENTPSV 144

Query: 80  VCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDKFGHIVTNYHVVSALATDNS 139
           V I NL +        ++A  +  D     +G+GSGF+WDK GHIVTNYHV+        
Sbjct: 145 VYITNLAV-------RQDAFTL--DVLEVPQGSGSGFVWDKQGHIVTNYHVI-------R 204

Query: 140 GLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQGHELKPIVFGTSRSLRVGQS 199
           G    +V L D      + DAK+VGFD + D+AVL+++   ++L+PI  G S  L VGQ 
Sbjct: 205 GASDLRVTLAD----QTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQK 264

Query: 200 CYAIGNPFGYEKTLTAGVISGLGREIPS-PNGRAIRGAIQTDAAISAGNSGGPLVDLYGH 259
            +AIGNPFG + TLT GVISGL REI S   GR I+  IQTDAAI+ GNSGGPL+D  G 
Sbjct: 265 VFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGT 324

Query: 260 VIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVYG 299
           +IG+NTA ++   +G SSGV F+IP+DTV   V  L+ +G
Sbjct: 325 LIGINTAIYS--PSGASSGVGFSIPVDTVGGIVDQLVRFG 330

[Close]

BLAST of CmaCh13G005250 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-41
Identity = 123/295 (41.69%), Postives = 165/295 (55.93%), Query Frame = 0


Query: 11  LPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDEV------PQE 70
           L V S P+++   L  S    + F P+  + S LA   PS A +  +   V         
Sbjct: 57  LAVKSVPSTTRRILLTSLFMNLCFNPSRYL-SALALGDPSVATVEDVSPTVFPAGPLFPT 116

Query: 71  EDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDKFGHI 130
           E RIV LF++ + SVV I   ++  +PQ      + I +       G GSG +WD  G+I
Sbjct: 117 EGRIVQLFEKNTYSVVNI--FDVTLRPQLKMTGVVEIPE-------GNGSGVVWDGQGYI 176

Query: 131 VTNYHVV-SALATDNS-GLQRCKVNLVDVKGNGISRDAKIVGFDPEYDLAVLKVELQGHE 190
           VTNYHV+ +AL+ + S G    +VN++   G   + + K+VG D   DLAVLKV+     
Sbjct: 177 VTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETL 236

Query: 191 LKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAA 250
           LKPI  G S SL+VGQ C AIGNPFG++ TLT GVISGL R+I S  G  I G IQTDAA
Sbjct: 237 LKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTGVTIGGGIQTDAA 296

Query: 251 ISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTVVRTVPYLIVY 298
           I+ GNSGGPL+D  G++IG+NTA FT+                TV++ VP LI +
Sbjct: 297 INPGNSGGPLLDSKGNLIGINTAIFTQ----------------TVLKIVPQLIQF 325

[Close]

BLAST of CmaCh13G005250 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 91.3 bits (225), Expect = 1.5e-18
Identity = 65/186 (34.95%), Postives = 98/186 (52.69%), Query Frame = 0


Query: 110 KGTGSGFIWDKFGHIVTNYHVVSALATDNSGLQRCKVNLVDVK-GNGISRDAKIVGFDPE 169
           K  GSG I D  G I+T  HVV     D   ++      VDV   +G + +  +V  D +
Sbjct: 146 KSIGSGTIIDADGTILTCAHVV----VDFQNIRHSSKGRVDVTLQDGRTFEGVVVNADLQ 205

Query: 170 YDLAVLKVELQGHELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSP 229
            D+A++K++ +   L     G S  LR G    A+G P   + T+TAG++S + R+    
Sbjct: 206 SDIALVKIKSK-TPLPTAKLGFSSKLRPGDWVIAVGCPLSLQNTVTAGIVSCVDRKSSDL 265

Query: 230 N-GRAIRGAIQTDAAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGMSSGVNFAIPIDTV 289
             G   R  +QTD +I+AGNSGGPLV+L G VIGVN           + G+ F++PID+V
Sbjct: 266 GLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVNIMKVL-----AADGLGFSVPIDSV 321

Query: 290 VRTVPY 294
            + + +
Sbjct: 326 SKIIEH 321

The following BLAST results are available for this feature:

BLAST of CmaCh13G005250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEL7	4.1e-98	61.15	Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 ...	[more]
Q9LU10	3.3e-47	44.75	Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ...	[more]
O22609	4.3e-47	43.93	Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ...	[more]
Q2SL36	9.6e-31	38.66	Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain...	[more]
Q4KGQ4	1.5e-28	40.00	Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fluorescens (s...	[more]

BLAST of CmaCh13G005250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KMS0	3.0e-168	100.00	protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1EKF8	5.2e-165	98.36	protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1EHG9	2.6e-148	91.48	protease Do-like 5, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1CEE6	6.0e-145	86.23	protease Do-like 5, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A5D3DAW0	2.3e-144	86.23	Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G0...	[more]

BLAST of CmaCh13G005250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023001449.1	6.1e-168	100.00	protease Do-like 5, chloroplastic isoform X1 [Cucurbita maxima]	[more]
XP_023520040.1	8.3e-165	97.70	protease Do-like 5, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022927238.1	1.1e-164	98.36	protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata]	[more]
KAG6583889.1	6.8e-159	96.04	Protease Do-like 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038893976.1	4.4e-150	89.51	protease Do-like 5, chloroplastic [Benincasa hispida]	[more]

BLAST of CmaCh13G005250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18370.1	2.9e-99	61.15	DEGP protease 5	[more]
AT5G39830.1	2.3e-48	44.75	Trypsin family protein with PDZ domain	[more]
AT3G27925.1	3.0e-48	43.93	DegP protease 1	[more]
AT5G39830.2	1.2e-41	41.69	Trypsin family protein with PDZ domain	[more]
AT5G27660.1	1.5e-18	34.95	Trypsin family protein with PDZ domain	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 305..305
None	No IPR available	PFAM	PF13365	Trypsin_2	coord: 113..262 e-value: 2.9E-33 score: 115.9
None	No IPR available	PANTHER	PTHR43343	PEPTIDASE S12	coord: 18..305
None	No IPR available	PANTHER	PTHR43343:SF6	PROTEASE DO-LIKE 5, CHLOROPLASTIC ISOFORM X1	coord: 18..305
IPR001940	Peptidase S1C	PRINTS	PR00834	PROTEASES2C	coord: 255..272 score: 39.43 coord: 233..250 score: 62.74 coord: 196..220 score: 48.88 coord: 122..134 score: 50.47
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 59..186 e-value: 1.3E-31 score: 110.8
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 187..299 e-value: 1.2E-39 score: 137.1
IPR009003	Peptidase S1, PA clan	SUPERFAMILY	50494	Trypsin-like serine proteases	coord: 70..291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh13G005250.1	CmaCh13G005250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0004252	serine-type endopeptidase activity