CmaCh13G000690 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G000690
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
LocationCma_Chr13: 553000 .. 554289 (+)
RNA-Seq ExpressionCmaCh13G000690
SyntenyCmaCh13G000690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGACGAATCAATGAACACAAATTTAGACGCAAATGGCGAACTGGTAGCTGTCATATTCGGAGTCACCGGGTTGGTCGGAAAAGAGTTGGCCAAAATGCTTCTCTCAACCGCTGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGATAATATCAGCCCCATCTCCGATCCCAACTTCCACTTCATTTCTTGCGATTTGCTCGACCGCCGTTCGGCTCACCTGAACCTCTCACCCGTCCGCCACGTCACCCACATGTTCTGGATCACTTGGGCCGCCCAGCTCCGGTTCGATTCCCAGGATTGCTCTGACCAGAACAAGGCCATGTTGGCCAACGCCCTCGATGCTATTCTCCCGTCGGCGTCGGCCCTCCGCCACGTCTCTCTGCAGACCGGGGTCAAGCATTACGCTTCTCTCACGAGCTCCACCAGCAGCGGCGGTGGGGAGGTGGTTTATAGTGAGGACAGCCCGAGAGCGGAGTCCGGGAATAATTTTTACTATGTGTTGGAGGATCTCCTCCGCGAGCGGCTGTCCGGCTACCGGAGAGTGGTGGCGTGGAGTATTCTCCGACCGGGTTTGATACTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGCGCGATTTGCAAGAAGTTGAAGATGCCATTTGTGTTCGGCGGCACGGCGGCGTGCTGGGAGGAGGTTTATATCGATGGCTCCGATGCTCGGCTCACGGCTGAGCAGCACGTGTGGGCCGCAACACAAGCAGTTGAAATAAATGGTACGTAACACCTACATCGAGCTGTCATAAATATAAATATAAATTTTTTTTTATAAGAGACAATTTCATTCCTAATTGATAAATATAAATATAAATTTAATATCTGTAAAAATACAGCCATCACAGACAGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAGCTTGGCGTGACGGTGGGGGCGGAGGACGAGAGGATGTTTTCGCCGGAGTTCCATTATACGGAAGCTATGGCGGAGAAAGGGATGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGGTTGCGACGGAAATGGGGGAGCTGGCGAATTGGGGATTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCCAAATCCGACCGGTTAGGGTTCACTGTAAAATACAAGATGTTGGACTCCATCTTGTATTGGATTGATGTCATGAGACACGACAAATTGATTCCGTCTCCTTAA

mRNA sequence

ATGGCGGACGAATCAATGAACACAAATTTAGACGCAAATGGCGAACTGGTAGCTGTCATATTCGGAGTCACCGGGTTGGTCGGAAAAGAGTTGGCCAAAATGCTTCTCTCAACCGCTGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGATAATATCAGCCCCATCTCCGATCCCAACTTCCACTTCATTTCTTGCGATTTGCTCGACCGCCGTTCGGCTCACCTGAACCTCTCACCCGTCCGCCACGTCACCCACATGTTCTGGATCACTTGGGCCGCCCAGCTCCGGTTCGATTCCCAGGATTGCTCTGACCAGAACAAGGCCATGTTGGCCAACGCCCTCGATGCTATTCTCCCGTCGGCGTCGGCCCTCCGCCACGTCTCTCTGCAGACCGGGGTCAAGCATTACGCTTCTCTCACGAGCTCCACCAGCAGCGGCGGTGGGGAGGTGGTTTATAGTGAGGACAGCCCGAGAGCGGAGTCCGGGAATAATTTTTACTATGTGTTGGAGGATCTCCTCCGCGAGCGGCTGTCCGGCTACCGGAGAGTGGTGGCGTGGAGTATTCTCCGACCGGGTTTGATACTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGCGCGATTTGCAAGAAGTTGAAGATGCCATTTGTGTTCGGCGGCACGGCGGCGTGCTGGGAGGAGGTTTATATCGATGGCTCCGATGCTCGGCTCACGGCTGAGCAGCACGTGTGGGCCGCAACACAAGCAGTTGAAATAAATGCCATCACAGACAGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAGCTTGGCGTGACGGTGGGGGCGGAGGACGAGAGGATGTTTTCGCCGGAGTTCCATTATACGGAAGCTATGGCGGAGAAAGGGATGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGGTTGCGACGGAAATGGGGGAGCTGGCGAATTGGGGATTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCCAAATCCGACCGGTTAGGGTTCACTGTAAAATACAAGATGTTGGACTCCATCTTGTATTGGATTGATGTCATGAGACACGACAAATTGATTCCGTCTCCTTAA

Coding sequence (CDS)

ATGGCGGACGAATCAATGAACACAAATTTAGACGCAAATGGCGAACTGGTAGCTGTCATATTCGGAGTCACCGGGTTGGTCGGAAAAGAGTTGGCCAAAATGCTTCTCTCAACCGCTGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGATAATATCAGCCCCATCTCCGATCCCAACTTCCACTTCATTTCTTGCGATTTGCTCGACCGCCGTTCGGCTCACCTGAACCTCTCACCCGTCCGCCACGTCACCCACATGTTCTGGATCACTTGGGCCGCCCAGCTCCGGTTCGATTCCCAGGATTGCTCTGACCAGAACAAGGCCATGTTGGCCAACGCCCTCGATGCTATTCTCCCGTCGGCGTCGGCCCTCCGCCACGTCTCTCTGCAGACCGGGGTCAAGCATTACGCTTCTCTCACGAGCTCCACCAGCAGCGGCGGTGGGGAGGTGGTTTATAGTGAGGACAGCCCGAGAGCGGAGTCCGGGAATAATTTTTACTATGTGTTGGAGGATCTCCTCCGCGAGCGGCTGTCCGGCTACCGGAGAGTGGTGGCGTGGAGTATTCTCCGACCGGGTTTGATACTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGCGCGATTTGCAAGAAGTTGAAGATGCCATTTGTGTTCGGCGGCACGGCGGCGTGCTGGGAGGAGGTTTATATCGATGGCTCCGATGCTCGGCTCACGGCTGAGCAGCACGTGTGGGCCGCAACACAAGCAGTTGAAATAAATGCCATCACAGACAGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAGCTTGGCGTGACGGTGGGGGCGGAGGACGAGAGGATGTTTTCGCCGGAGTTCCATTATACGGAAGCTATGGCGGAGAAAGGGATGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGGTTGCGACGGAAATGGGGGAGCTGGCGAATTGGGGATTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCCAAATCCGACCGGTTAGGGTTCACTGTAAAATACAAGATGTTGGACTCCATCTTGTATTGGATTGATGTCATGAGACACGACAAATTGATTCCGTCTCCTTAA

Protein sequence

MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Homology
[Close]
BLAST of CmaCh13G000690 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 5.4e-63
Identity = 152/377 (40.32%), Postives = 200/377 (53.05%), Query Frame = 0 

Query: 17  VAVIFGVTGLVGKELAKML--LSTAG--WKVYGVARRPDNISPISDPNFHFISCDLLDRR 76
           VA+I GVTG+VG  LA++L    T G  WKVYGVARRP        P   +I CD+ D  
Sbjct: 28  VALIIGVTGIVGNSLAEILPISDTPGGPWKVYGVARRPRPAWNADHP-VEYIQCDISDAS 87

Query: 77  SAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSASALRHVSLQT 136
             H  LSP+  VTH+FW+TWA   R    +C + N  ML N L+A++P A+ L H+ LQT
Sbjct: 88  DTHTKLSPLTDVTHIFWVTWAN--RPTESECCELNGTMLRNVLNALIPKAANLHHICLQT 147

Query: 137 GVKHY-ASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWSILR 196
           G KHY     +       E  ++ED PR  +  NFYY LED+L E  S  +  + WS+ R
Sbjct: 148 GHKHYIGPFEAFGKIKPHEPPFTEDMPRL-NAPNFYYTLEDMLVE-ASEKKAGLNWSVHR 207

Query: 197 PGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHV 256
           P +I G S  +  N +G+LCVY AICK    P  F GT A W   Y   SDA L AE  +
Sbjct: 208 PAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAW-NCYSVASDADLIAEHQI 267

Query: 257 WAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHYTEA 316
           WAA     ++     EAFN  NG  F WK +W  +AE+ GV     DE          E 
Sbjct: 268 WAA-----VDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVEYAEFDES--EKPTSLVER 327

Query: 317 MAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKML 376
           M +KG VW EIV E GL  T++  +A W F D +    E ++ S  KS   G+       
Sbjct: 328 MKDKGPVWEEIVRENGLHTTKLEGVATWWFADVILG-GECLLDSMNKSKEHGYLGFRNTK 387

Query: 377 DSILYWIDVMRHDKLIP 389
           +S++  ID M+  K++P
Sbjct: 388 NSLISVIDKMKAHKIVP 390

[Close]
BLAST of CmaCh13G000690 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.9e-61
Identity = 148/380 (38.95%), Postives = 202/380 (53.16%), Query Frame = 0 

Query: 13  NGELVAVIFGVTGLVGKELAKM--LLSTAG--WKVYGVARRPDNISPISDPNFHFISCDL 72
           N E VA+I GVTG+VG  LA++  L  T G  WKVYGVARRP   S   D    +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPSWNEDHPITYISCDV 83

Query: 73  LDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSASALRHV 132
           LD       LSP+  VTH+F+ TW    R   ++  + N  ML N L+A++P+   L+H+
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTK--RSTEKENCEANGKMLKNVLNAMIPNCPNLKHI 143

Query: 133 SLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWS 192
            LQTG KHY        S   +   +ED PR +S  N+YY  ED+L E +   +  + WS
Sbjct: 144 CLQTGRKHYLGAFEDLKSKCHDPPLTEDLPRLDS-QNYYYTQEDILFEEVQ-KKEGLTWS 203

Query: 193 ILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAE 252
           + RPG I G S  +  N +G+LCVY AICK       F G    W+  Y D SDA L AE
Sbjct: 204 VHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAWDG-YSDCSDADLIAE 263

Query: 253 QHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHY 312
             +WAA     ++     EAFNV NG  F WK  W  +AE+ GV  G  +E     +   
Sbjct: 264 HQIWAA-----VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVECGEYEE---GQQVKL 323

Query: 313 TEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKY 372
            + M +KG VW +IV E GL  T++ ++ NW F D +    E  + +  KS   GF    
Sbjct: 324 QDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKEHGFLGFR 383

Query: 373 KMLDSILYWIDVMRHDKLIP 389
              +S + WID ++  K++P
Sbjct: 384 NSKNSFISWIDKVKAFKIVP 388

[Close]
BLAST of CmaCh13G000690 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 5.1e-61
Identity = 147/380 (38.68%), Postives = 199/380 (52.37%), Query Frame = 0 

Query: 13  NGELVAVIFGVTGLVGKELAKML----LSTAGWKVYGVARRPDNISPISDPNFHFISCDL 72
           N E VA+I GVTG+VG  LA++L         WKVYGVARRP   S   D    +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYGVARRP-RPSWNEDHPITYISCDV 83

Query: 73  LDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSASALRHV 132
           LD       LSP+  VTH+F+ TW    R   ++  + N  ML N L+A++P+   L+H+
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTK--RSTEKENCEANGKMLKNVLNAMIPNCPNLKHI 143

Query: 133 SLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWS 192
            LQTG KHY            +   +ED PR +S  NFYY  ED+L E +   +  + WS
Sbjct: 144 CLQTGRKHYLGAFEDWKIKRHDPPLTEDLPRLDS-QNFYYTQEDILFEEVQ-KKESLTWS 203

Query: 193 ILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAE 252
           + RPG I G S  +  N +G+LCVY AICK       F G    W+  Y D SDA L AE
Sbjct: 204 VHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAWDG-YSDCSDADLIAE 263

Query: 253 QHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHY 312
             +WAA     ++     EAFNV NG  F WK  W  +AE+ GV  G  +E     E   
Sbjct: 264 HQIWAA-----VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVECGEYEE---GKEVKL 323

Query: 313 TEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKY 372
            + M +KG VW +IV E GL  T++ ++ NW F D +    E  + +  KS   GF    
Sbjct: 324 QDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKEHGFLGFR 383

Query: 373 KMLDSILYWIDVMRHDKLIP 389
              +S + WID ++  K++P
Sbjct: 384 NSKNSFISWIDKVKAFKIVP 388

[Close]
BLAST of CmaCh13G000690 vs. ExPASy Swiss-Prot
Match: A0A221J5X1 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.5e-60
Identity = 149/392 (38.01%), Postives = 209/392 (53.32%), Query Frame = 0 

Query: 3   DESMNTNLDANGELVAVIFGVTGLVGKELAKM--LLSTAG--WKVYGVARRPDNISPISD 62
           DES    L    + VA+I GVTGLVG  LA+M  L  T G  WKVYGVARRP   S   D
Sbjct: 16  DESSAVKLQQQ-QCVALIVGVTGLVGNSLAEMLPLSDTPGGPWKVYGVARRP-RPSWNED 75

Query: 63  PNFHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAI 122
              ++ISCD+ +       L P+  VTH+F+ TW +  R   ++  + N  ML N LD +
Sbjct: 76  HPINYISCDVSNTAEVEAKLPPLSDVTHIFYATWTS--RSSEEENCEANGKMLKNVLDTM 135

Query: 123 LPSASALRHVSLQTGVKHY-ASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRER 182
           +P+   L+H+ LQTG  HY AS+     +G  +   +ED PR  + NNFYY  ED+L E 
Sbjct: 136 IPNCPNLKHICLQTGRFHYVASVVDWKINGSHDTPLTEDLPRLNT-NNFYYTQEDILLEE 195

Query: 183 LSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVY 242
           +   +  + WS+ RPG I G S  +  N +G+LCVY AICK+      F G    W+  Y
Sbjct: 196 VK-RKEGLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKQEGAVLRFPGCKGAWDG-Y 255

Query: 243 IDGSDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAE 302
            D +DA L AE ++WAA     ++     ++FNV NG  F WK +W  +AE+ GV  G  
Sbjct: 256 SDCADADLIAEHYIWAA-----LDPHAKNQSFNVSNGDVFKWKHLWKVLAEQFGVECGGY 315

Query: 303 DERMFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRA 362
            E     +    + M +KG VW +IV E GL  T++ ++  W F D++    E  + S  
Sbjct: 316 -EYEEGQQVRLQDVMKDKGPVWDKIVRENGLSNTKLEDVGKWWFSDTIL-WNECRLDSMN 375

Query: 363 KSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 390
           KS   GF       +  LYWI  ++  K++PS
Sbjct: 376 KSKEHGFLGFRNSKNCFLYWIHKLKAYKIVPS 393

[Close]
BLAST of CmaCh13G000690 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.5e-60
Identity = 146/378 (38.62%), Postives = 200/378 (52.91%), Query Frame = 0 

Query: 15  ELVAVIFGVTGLVGKELAKM--LLSTAG--WKVYGVARRPDNISPISDPNFHFISCDLLD 74
           E VA+I GVTG+VG  LA++  L  T G  WKVYGVARRP        P   +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 85

Query: 75  RRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSASALRHVSL 134
                  LSP+  VTH+F++TW  +   +S++C + N +ML N L AI+P A  LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWSIL 194
           QTG KHY    ++      +  ++ED PR +   NFYY  ED+L E +      V WSI 
Sbjct: 146 QTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIKKI-ETVTWSIH 205

Query: 195 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQH 254
           RP +I G S  +  N +G+LCVY AICK    P +F G+   WE  ++  SDA L AEQ 
Sbjct: 206 RPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEG-FMTASDADLIAEQQ 265

Query: 255 VWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHYTE 314
           +WAA     ++     EAFN  N   F WK +W  +AE+ G+     +E          E
Sbjct: 266 IWAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE---GKNLGLVE 325

Query: 315 AMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKM 374
            M  K  VW E+V+E  L   ++ E+  W F D +  + E M+ S  KS   GF      
Sbjct: 326 MMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLGFRNS 385

Query: 375 LDSILYWIDVMRHDKLIP 389
            +S + WID  +  K++P
Sbjct: 386 NNSFISWIDKYKAFKIVP 388

[Close]
BLAST of CmaCh13G000690 vs. ExPASy TrEMBL
Match: A0A6J1I0N6 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111469437 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.1e-227
Identity = 390/390 (100.00%), Postives = 390/390 (100.00%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180
           SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG
Sbjct: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240
           YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG
Sbjct: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240

Query: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300
           SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER
Sbjct: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300

Query: 301 MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360
           MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD
Sbjct: 301 MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 391
           RLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]
BLAST of CmaCh13G000690 vs. ExPASy TrEMBL
Match: A0A6J1HM54 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111464842 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 4.7e-219
Identity = 377/390 (96.67%), Postives = 381/390 (97.69%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISCDLLDRRSAHLNLSP+ HVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLGHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180
           SA ALRHVSLQTGVKHYASLTSST SGGGEVVYSE+SPRAESGNNFYYVLEDLLRERLSG
Sbjct: 121 SAPALRHVSLQTGVKHYASLTSSTGSGGGEVVYSENSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240
            RRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYID 
Sbjct: 181 RRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDS 240

Query: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300
           SDARLTAEQHVWAAT+AVEINA+TD EAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER
Sbjct: 241 SDARLTAEQHVWAATKAVEINALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300

Query: 301 MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360
           MFSPEF YT AMAEKG VWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD
Sbjct: 301 MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 391
           RLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]
BLAST of CmaCh13G000690 vs. ExPASy TrEMBL
Match: A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 4.0e-194
Identity = 341/396 (86.11%), Postives = 357/396 (90.15%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MA E +NTNLDANGELVAVIFGVTGLVGK+LAK LLSTAGWKVYGVARRPDN+SPIS P 
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISCDLLDRRS   NLSPVRHVTH+FWITWAAQLR DS DCSDQN+AMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTS----SGGGEVVYSEDSPRAESGNNFYYVLEDLLRE 180
           SA ALRHVSLQTG+KHYASLT   +     G GEVVY EDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLS  RR+VAWS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGV-TVG 300
           YIDGSDARLTAEQH+W AT+AVEINA  D EAFNVCNGWSF WKEIWGAVAEKLGV T G
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AED--ERMFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMV 360
           AE+  ERMFS EFHYT AM +KG VWAEIVEEEGLV TEM ELANWGFLD+LFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 390
           ASRAKSDRLGF VKYKMLDS+LYWIDVMR+DKLIPS
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIPS 396

[Close]
BLAST of CmaCh13G000690 vs. ExPASy TrEMBL
Match: A0A5D3BGL7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001240 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.5e-190
Identity = 335/393 (85.24%), Postives = 351/393 (89.31%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MA + +NTNLDANGELVAVIFGVTGLVGK+LAK LLSTAGWKVYGVARRPDN+SPIS P 
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISCDLLDR+S   NLSPVRHVTH+FWITWAAQLR DS  CSDQN+AMLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTSSGGG--EVVYSEDSPRAESGNNFYYVLEDLLRERL 180
           SA ALRHVSLQTGVKHYASLT   +  GG  EVVY EDSPRAESGNNFYYVLEDLLRERL
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL 180

Query: 181 SGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240
           S  RR+VAWS+LRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI
Sbjct: 181 SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240

Query: 241 DGSDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAED 300
           DGSDARLTAEQH+W AT+AVEINA  D EAFNVCNGWSF WKEIWG VAEKLGV   AE+
Sbjct: 241 DGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE 300

Query: 301 --ERMFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASR 360
             ERMFS EFHYT AM +KG VWAEIVEEEGLVATEM ELANW FLD+LFRLPEKMVASR
Sbjct: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360

Query: 361 AKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 390
           AKSDR GF VKYKMLDSILYWIDVMR+DKLIPS
Sbjct: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

[Close]
BLAST of CmaCh13G000690 vs. ExPASy TrEMBL
Match: A0A1S3C4Y3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497085 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.5e-190
Identity = 335/393 (85.24%), Postives = 351/393 (89.31%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MA + +NTNLDANGELVAVIFGVTGLVGK+LAK LLSTAGWKVYGVARRPDN+SPIS P 
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISCDLLDR+S   NLSPVRHVTH+FWITWAAQLR DS  CSDQN+AMLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTSSGGG--EVVYSEDSPRAESGNNFYYVLEDLLRERL 180
           SA ALRHVSLQTGVKHYASLT   +  GG  EVVY EDSPRAESGNNFYYVLEDLLRERL
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL 180

Query: 181 SGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240
           S  RR+VAWS+LRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI
Sbjct: 181 SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240

Query: 241 DGSDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAED 300
           DGSDARLTAEQH+W AT+AVEINA  D EAFNVCNGWSF WKEIWG VAEKLGV   AE+
Sbjct: 241 DGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE 300

Query: 301 --ERMFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASR 360
             ERMFS EFHYT AM +KG VWAEIVEEEGLVATEM ELANW FLD+LFRLPEKMVASR
Sbjct: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360

Query: 361 AKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 390
           AKSDR GF VKYKMLDSILYWIDVMR+DKLIPS
Sbjct: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

[Close]
BLAST of CmaCh13G000690 vs. NCBI nr
Match: XP_022970466.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 797.7 bits (2059), Expect = 4.4e-227
Identity = 390/390 (100.00%), Postives = 390/390 (100.00%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180
           SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG
Sbjct: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240
           YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG
Sbjct: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240

Query: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300
           SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER
Sbjct: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300

Query: 301 MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360
           MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD
Sbjct: 301 MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 391
           RLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]
BLAST of CmaCh13G000690 vs. NCBI nr
Match: XP_022964858.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])

HSP 1 Score: 770.0 bits (1987), Expect = 9.8e-219
Identity = 377/390 (96.67%), Postives = 381/390 (97.69%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISCDLLDRRSAHLNLSP+ HVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLGHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180
           SA ALRHVSLQTGVKHYASLTSST SGGGEVVYSE+SPRAESGNNFYYVLEDLLRERLSG
Sbjct: 121 SAPALRHVSLQTGVKHYASLTSSTGSGGGEVVYSENSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240
            RRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYID 
Sbjct: 181 RRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDS 240

Query: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300
           SDARLTAEQHVWAAT+AVEINA+TD EAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER
Sbjct: 241 SDARLTAEQHVWAATKAVEINALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300

Query: 301 MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360
           MFSPEF YT AMAEKG VWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD
Sbjct: 301 MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 391
           RLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]
BLAST of CmaCh13G000690 vs. NCBI nr
Match: KAG6583366.1 ((S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 764.2 bits (1972), Expect = 5.4e-217
Identity = 373/390 (95.64%), Postives = 378/390 (96.92%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MADESMNTNLDANGELVAVIFGVTGLVGKELAK+LLSTAGWKVYGVARRPDNISPISDPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKLLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISCDLLDRRSAHLNLSP+ HVTHMFWITWAAQLRFDSQDCS+QNKAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLCHVTHMFWITWAAQLRFDSQDCSNQNKAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180
           SA ALRHVSLQTGVKHYASLT ST  GGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG
Sbjct: 121 SAPALRHVSLQTGVKHYASLTGSTGGGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240
            RRVVAWSILRPGLILGCSNRTFFNFMGS+CVYGAICKKLKMPFVFGGT ACWEEVYIDG
Sbjct: 181 RRRVVAWSILRPGLILGCSNRTFFNFMGSVCVYGAICKKLKMPFVFGGTGACWEEVYIDG 240

Query: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300
           SDARLTAEQHVWAAT+AVEINAITD EAFNVCNGWSFTWKEIWGAVAEKLGVT GAEDER
Sbjct: 241 SDARLTAEQHVWAATKAVEINAITDGEAFNVCNGWSFTWKEIWGAVAEKLGVTAGAEDER 300

Query: 301 MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360
           MFSPEF YT AMAEKG VWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD
Sbjct: 301 MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 391
           RLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]
BLAST of CmaCh13G000690 vs. NCBI nr
Match: KAG7019139.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 763.8 bits (1971), Expect = 7.0e-217
Identity = 374/390 (95.90%), Postives = 377/390 (96.67%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MADESMNTNLDANGELVAVIFGVTGLVGKELAK+LLSTAGWKVYGVARRPDNISPISDPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKLLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISCDLLDRRSAHLNLSP+ HVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLCHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180
           SA ALRHVSLQTGVKHYASLT ST  GGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG
Sbjct: 121 SAPALRHVSLQTGVKHYASLTGSTGGGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240
            RRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG
Sbjct: 181 RRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240

Query: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300
           SDARLTAEQHVWA T+AVEINAIT  EAFNVCNGWSFTWKEIWGAVAEKLGVT GAEDER
Sbjct: 241 SDARLTAEQHVWATTKAVEINAITYGEAFNVCNGWSFTWKEIWGAVAEKLGVTAGAEDER 300

Query: 301 MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360
           MFSPEF YT AMAEKG VWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD
Sbjct: 301 MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 391
           RLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]
BLAST of CmaCh13G000690 vs. NCBI nr
Match: XP_023519075.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 759.6 bits (1960), Expect = 1.3e-215
Identity = 373/390 (95.64%), Postives = 377/390 (96.67%), Query Frame = 0 

Query: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60
           MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120
           FHFISC+LLDRRSAHLNLSP+RHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP
Sbjct: 61  FHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180
           SA ALRHVSLQTGVKHYASLTSST  GGGEVVY EDSPRAES NNFYYVLEDLLRERLSG
Sbjct: 121 SALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRAESVNNFYYVLEDLLRERLSG 180

Query: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240
           YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKL MPFVFGGTAACWEEVYIDG
Sbjct: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLNMPFVFGGTAACWEEVYIDG 240

Query: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300
           SDARLTAEQHVWAAT+AVEIN   D EAFNVCNGWSFTWKEIWGAVAEKLGVT GAEDER
Sbjct: 241 SDARLTAEQHVWAATKAVEIN---DGEAFNVCNGWSFTWKEIWGAVAEKLGVTAGAEDER 300

Query: 301 MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360
           MFSPEFHYT AMAEKG VWAEIVEEEGLVAT+MGELANWGFLDSLFRLPEKMVASRAKSD
Sbjct: 301 MFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 391
           RLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIPSP 387

[Close]
BLAST of CmaCh13G000690 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 406.8 bits (1044), Expect = 2.0e-113
Identity = 208/379 (54.88%), Postives = 260/379 (68.60%), Query Frame = 0 

Query: 15  ELVAVIFGVTGLVGKELAK-MLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRS 74
           E VA+IFGVTGLVG+E+ K +L+S  GW++YGVAR P+  S      ++FISCDLL+   
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPEINS--MTKMYNFISCDLLNASE 75

Query: 75  AHLNLSPVRH-VTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSASALRHVSLQT 134
               LSP++  V+H+FW+TW+ +   D+ +C  QNK ML NALDAILP+A  L+H SLQT
Sbjct: 76  TKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQT 135

Query: 135 GVKHYASLTSST---SSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWSI 194
           G+KHY SL   T     G     YSE+ PR  SG NFYYVLEDLL+E+++  R  V WS+
Sbjct: 136 GMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKIT--RSSVVWSV 195

Query: 195 LRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQ 254
            RPGL++G S+RT +NFMGSLCVYGA+CK L +PFVFGGT  CWEE YIDGSD+ L AEQ
Sbjct: 196 QRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAEQ 255

Query: 255 HVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHYT 314
           H++AAT           EAFN  NG  FTWKEIW  + +KLGV V   +  MF   F + 
Sbjct: 256 HIFAATSG---KVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQV--NETTMFDEGFWFG 315

Query: 315 EAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYK 374
             M E+  VW EIV +E LV TE+ +LANW FLD+LFR P K++  R K DR GF  KY+
Sbjct: 316 REMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYR 375

Query: 375 MLDSILYWIDVMRHDKLIP 389
            LDS+LYWIDVMR +KLIP
Sbjct: 376 TLDSVLYWIDVMRDEKLIP 385

[Close]
BLAST of CmaCh13G000690 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 1.1e-61
Identity = 146/378 (38.62%), Postives = 200/378 (52.91%), Query Frame = 0 

Query: 15  ELVAVIFGVTGLVGKELAKM--LLSTAG--WKVYGVARRPDNISPISDPNFHFISCDLLD 74
           E VA+I GVTG+VG  LA++  L  T G  WKVYGVARRP        P   +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 85

Query: 75  RRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSASALRHVSL 134
                  LSP+  VTH+F++TW  +   +S++C + N +ML N L AI+P A  LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWSIL 194
           QTG KHY    ++      +  ++ED PR +   NFYY  ED+L E +      V WSI 
Sbjct: 146 QTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIKKI-ETVTWSIH 205

Query: 195 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQH 254
           RP +I G S  +  N +G+LCVY AICK    P +F G+   WE  ++  SDA L AEQ 
Sbjct: 206 RPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEG-FMTASDADLIAEQQ 265

Query: 255 VWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHYTE 314
           +WAA     ++     EAFN  N   F WK +W  +AE+ G+     +E          E
Sbjct: 266 IWAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE---GKNLGLVE 325

Query: 315 AMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKM 374
            M  K  VW E+V+E  L   ++ E+  W F D +  + E M+ S  KS   GF      
Sbjct: 326 MMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLGFRNS 385

Query: 375 LDSILYWIDVMRHDKLIP 389
            +S + WID  +  K++P
Sbjct: 386 NNSFISWIDKYKAFKIVP 388

[Close]
BLAST of CmaCh13G000690 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 1.1e-61
Identity = 146/378 (38.62%), Postives = 200/378 (52.91%), Query Frame = 0 

Query: 15  ELVAVIFGVTGLVGKELAKM--LLSTAG--WKVYGVARRPDNISPISDPNFHFISCDLLD 74
           E VA+I GVTG+VG  LA++  L  T G  WKVYGVARRP        P   +I CD+ D
Sbjct: 25  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 84

Query: 75  RRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSASALRHVSL 134
                  LSP+  VTH+F++TW  +   +S++C + N +ML N L AI+P A  LRHV L
Sbjct: 85  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 144

Query: 135 QTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWSIL 194
           QTG KHY    ++      +  ++ED PR +   NFYY  ED+L E +      V WSI 
Sbjct: 145 QTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIKKI-ETVTWSIH 204

Query: 195 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQH 254
           RP +I G S  +  N +G+LCVY AICK    P +F G+   WE  ++  SDA L AEQ 
Sbjct: 205 RPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEG-FMTASDADLIAEQQ 264

Query: 255 VWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHYTE 314
           +WAA     ++     EAFN  N   F WK +W  +AE+ G+     +E          E
Sbjct: 265 IWAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE---GKNLGLVE 324

Query: 315 AMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKM 374
            M  K  VW E+V+E  L   ++ E+  W F D +  + E M+ S  KS   GF      
Sbjct: 325 MMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLGFRNS 384

Query: 375 LDSILYWIDVMRHDKLIP 389
            +S + WID  +  K++P
Sbjct: 385 NNSFISWIDKYKAFKIVP 387

The following BLAST results are available for this feature:
BLAST of CmaCh13G000690 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A0A1C9CX665.4e-6340.32(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC... [more]
A0A221J5W83.9e-6138.95(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ... [more]
A0A221J5X35.1e-6138.68(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1... [more]
A0A221J5X11.5e-6038.01(S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 ... [more]
Q9STX21.5e-6038.623-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... [more]
BLAST of CmaCh13G000690 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1I0N62.1e-227100.003-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HM544.7e-21996.673-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A0A0LVI24.0e-19486.11Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P... [more]
A0A5D3BGL73.5e-19085.243-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa... [more]
A0A1S3C4Y33.5e-19085.243-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo OX=3656 GN=... [more]
BLAST of CmaCh13G000690 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022970466.14.4e-227100.003-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima][more]
XP_022964858.19.8e-21996.673-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata][more]
KAG6583366.15.4e-21795.64(S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. ... [more]
KAG7019139.17.0e-21795.903-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp... [more]
XP_023519075.11.3e-21595.643-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo][more]
BLAST of CmaCh13G000690 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G58750.12.0e-11354.88NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.11.1e-6138.62NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.21.1e-6138.62NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 19..87
e-value: 2.8E-5
score: 23.7
NoneNo IPR availableGENE3D3.40.50.720coord: 11..388
e-value: 7.2E-97
score: 326.7
NoneNo IPR availablePANTHERPTHR32487:SF123-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE ISOFORM X1coord: 1..388
NoneNo IPR availablePANTHERPTHR324873-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASEcoord: 1..388
NoneNo IPR availableCDDcd089485beta-POR_like_SDR_acoord: 17..347
e-value: 7.82298E-94
score: 281.827
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 17..380

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G000690.1CmaCh13G000690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity