CmaCh13G000340 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G000340
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionLaccase
LocationCma_Chr13: 392225 .. 395109 (+)
RNA-Seq ExpressionCmaCh13G000340
SyntenyCmaCh13G000340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAGAGTGATGGAGGAGCTGCTTAAGATCAAGTCCTTTTGCTCTTTCTTCTTGCTTTCTCTTTTGGCCTTCCTCCTTTTACTCCCTCCTTTTGCTGTTGCAGAAACTCACTACCACGAGTTCATTGTAAGTTCTTAAATTCTCCCCTTTTTATTATATTCATATTGTCACGTTCGAGTAAACGAGTATATGATCTTAAAAATACAAGACGATCATGGATGCGAGATTCCACATCCATTGGAGTGGGGAACAAAACACTTTATAAGGGGTGAAAATCTCTCCTTACCTGGAAGGGAAAGTCCAAAGAAGGCAATATCTAGTGGTGGGCTTGGGCCGTTACAAATTGGTATCAAGTCGGATACTAGGCCATGTGCTAATGAGAACACTGGCCCCAAAAGGGTGGATTGTGAGATCCCACGTCGATGGGAGAGAGGAACGAAATATTCTTTATAAGGGAGCAGAAACCTTTTTCTAACATACATGTTTTTTAAACCTTGAGGAAACATGGTAAAAAAACTAGTGTTAGTCTGTGAGAGGAGTAGTTTTTACGGTTACAAGCAACCAATAGGTAATATGATGATGTGGCAAAAAAGGAAACCAAACCGGATTTCTCAGTTTGTTTGTCTCTGTTTGTATGCAAAAAAAAATGAACTGCAGGTGCAACCAAAGCCGGTGAAGAGGCTGTGCAAAGTCCACAACATCATAACAGTGAACGGGAAGTTCCCTGGCCCAACTCTATCGGTCAGAAACGGCGATTCCCTTGTCATCAAAGTAGTGAATGTTGCCCGCTACAATGTCTCTCTCCATTGGTACGAACACAATCTTTCCATTTGATGGGCATACAAAACTGAGTGGTAATTAAATGGTTGATTTGTATGGTTAGGCATGGGATCAGGCAGCTGAGGAACCCATGGGCAGATGGGCCAGAGTTCGTGACCCAATGCTCCATAAAGCCAGGAGGCAGCTACACCTACAGGTTCACCATTCAAGACCAAGAGGGAACCCTGTGGTGGCATGCTCATAGCCGATGGCTTCGAGCCACCGTCTATGGCGCTCTCATTATCTACCCCAAGCTCCCCTCTTCATACCCTTTCTTAATGCCCAAAAGAGAAGCCTCTGTTCTTCTTGGTAACCGCAGTCTCTTAATAAATGCTTAACAAATGCTCGCAATGGCTTATTTATGGTTTCTTGAACAGGGGAATGGTTCGACAGAGACCCGATAAGTGTGCTGCGGCAGGCGCTTTTCACTGGCGCTGCTCCTAATGTCTCTGATGCCTACACCATCAACGGCCAGCCCGGCGACTTCTACCGCTGCTCCGGCAAAGGTTGGTGCTTGGTGCTGTCTTCTTTATTACAAATTTGGAATGGGTTTTGGTTACGATGAAACAAAAAGGGTTTTGGTTTGATATTTGGCAGAGACGGTGAGATTCGCGGTGGATTCCGGCGAGACAATTCTTTTGAGGATCATAAACTCAGCATTGAATCAGGAGCTATTCTTCTCCGTCGCGAACCACCAATTGACGGTGGTGGCCGTCGACGCGTCTTACACCAAACCATTCACAACAAATGTGATTATGATCGGACCCGGGCAGACCACCGATGTCCTCCTGACAGCCAACCAACAACCAGCTCACTACTACATGGCTGCCACCGCCTACAACACCGCCCTCAACGCCGCCTTCGACAACACCACCACCACTGCCATCCTCGAATACAAAAACTGCCAGCAACACCAGCAGCAGCCACCGCGACCCACTATGGCTCAGCTCCCAAATTTCAACGACACCCCCACCGCCACCCGATTCACCTCCCAACTCCGGAGCCCCAACAGAGTGAACGTCCCGACCCAAATCGACAACAGCTTACTCTTCACCGTCGGATTAGGCCTCATCAACTGCACGAACCCAAACAGCCCCCGCTGCCAAGGCCCCAACGGCACTCGCTTTGCCGCCAGCATCAACAACGTCTCCTTCGTTTTCCCAAAATCCAATTCCATCATGCAAGCCTTTTACCAGGGCGTTCCGGGGGTTTTCACCACCGACTTTCCGCCGGTTCCGCCGCTGCAATTTGATTACACAGGGAACGTGAGCAGAGGGCTTTGGCAGCCCGGTCGAGGGACCAAGGGTTATAGGCTTAAATATGGATCAAATGTGCAGATTGTGCTGCAGGACACCAGCATTGTAACCACCGAAGACCACCCGATGCATCTCCATGGCTACCATTTCTACGTCGTTGGTTCGGGTTTTGGCAACTTCAACCCTGCGACGGATCCGGCGAGGTTCAATCTCATCGACCCACCGGTGAGAAACACCATTGGAACGCCCCCTGGTGGTTGGGTTGCCATTCGATTCGTGGCTGATAATCCAGGTAAGCTCCGATTGCAACAAATAAATCTGTGTTCTATATAATTAAATGGTTTTGAATTCCCCGTAATAAATGGATTGCGCGCTGAAAAACAGGGGCTTGGTTGATGCATTGCCATATAGACTCGCACCTTGCTTGGGGATTGGCGATGGTGTTTCTGGTTGAGAATGGCGAAGGAGAGCTGCAGTCTGTTCTTCCTCCCCCGCCGGATCTGCCTCCTTGTTAAGAACATCTGTATACTATACATCGGTTCAACCTCTCAATCATATAATCTTTGTTATTATTTTGGTGGATTGGAAGCAAGTACAACATATATGGCGAAGCTTTTAATTTTGAGCATTAAGGATAAACAAAATCTCTCTAGGTTGATTTCGTGTATTTTGGGGTGTCGTATATTTTGGATCTAAATGATTGTTCTTTAACAAGGTTGTTAGATCAAATGACAAGAGTTTCAAAACTAATCTGTTCTAATGTGTTCCCTATGACTACAAAATGGTTCAAAAGTGGTTAAACAGTGTTTTG

mRNA sequence

ACAGAGTGATGGAGGAGCTGCTTAAGATCAAGTCCTTTTGCTCTTTCTTCTTGCTTTCTCTTTTGGCCTTCCTCCTTTTACTCCCTCCTTTTGCTGTTGCAGAAACTCACTACCACGAGTTCATTGTGCAACCAAAGCCGGTGAAGAGGCTGTGCAAAGTCCACAACATCATAACAGTGAACGGGAAGTTCCCTGGCCCAACTCTATCGGTCAGAAACGGCGATTCCCTTGTCATCAAAGTAGTGAATGTTGCCCGCTACAATGTCTCTCTCCATTGGCATGGGATCAGGCAGCTGAGGAACCCATGGGCAGATGGGCCAGAGTTCGTGACCCAATGCTCCATAAAGCCAGGAGGCAGCTACACCTACAGGTTCACCATTCAAGACCAAGAGGGAACCCTGTGGTGGCATGCTCATAGCCGATGGCTTCGAGCCACCGTCTATGGCGCTCTCATTATCTACCCCAAGCTCCCCTCTTCATACCCTTTCTTAATGCCCAAAAGAGAAGCCTCTGTTCTTCTTGGGGAATGGTTCGACAGAGACCCGATAAGTGTGCTGCGGCAGGCGCTTTTCACTGGCGCTGCTCCTAATGTCTCTGATGCCTACACCATCAACGGCCAGCCCGGCGACTTCTACCGCTGCTCCGGCAAAGAGACGGTGAGATTCGCGGTGGATTCCGGCGAGACAATTCTTTTGAGGATCATAAACTCAGCATTGAATCAGGAGCTATTCTTCTCCGTCGCGAACCACCAATTGACGGTGGTGGCCGTCGACGCGTCTTACACCAAACCATTCACAACAAATGTGATTATGATCGGACCCGGGCAGACCACCGATGTCCTCCTGACAGCCAACCAACAACCAGCTCACTACTACATGGCTGCCACCGCCTACAACACCGCCCTCAACGCCGCCTTCGACAACACCACCACCACTGCCATCCTCGAATACAAAAACTGCCAGCAACACCAGCAGCAGCCACCGCGACCCACTATGGCTCAGCTCCCAAATTTCAACGACACCCCCACCGCCACCCGATTCACCTCCCAACTCCGGAGCCCCAACAGAGTGAACGTCCCGACCCAAATCGACAACAGCTTACTCTTCACCGTCGGATTAGGCCTCATCAACTGCACGAACCCAAACAGCCCCCGCTGCCAAGGCCCCAACGGCACTCGCTTTGCCGCCAGCATCAACAACGTCTCCTTCGTTTTCCCAAAATCCAATTCCATCATGCAAGCCTTTTACCAGGGCGTTCCGGGGGTTTTCACCACCGACTTTCCGCCGGTTCCGCCGCTGCAATTTGATTACACAGGGAACGTGAGCAGAGGGCTTTGGCAGCCCGGTCGAGGGACCAAGGGTTATAGGCTTAAATATGGATCAAATGTGCAGATTGTGCTGCAGGACACCAGCATTGTAACCACCGAAGACCACCCGATGCATCTCCATGGCTACCATTTCTACGTCGTTGGTTCGGGTTTTGGCAACTTCAACCCTGCGACGGATCCGGCGAGGTTCAATCTCATCGACCCACCGGTGAGAAACACCATTGGAACGCCCCCTGGTGGTTGGGTTGCCATTCGATTCGTGGCTGATAATCCAGGGGCTTGGTTGATGCATTGCCATATAGACTCGCACCTTGCTTGGGGATTGGCGATGGTGTTTCTGGTTGAGAATGGCGAAGGAGAGCTGCAGTCTGTTCTTCCTCCCCCGCCGGATCTGCCTCCTTGTTAAGAACATCTGTATACTATACATCGGTTCAACCTCTCAATCATATAATCTTTGTTATTATTTTGGTGGATTGGAAGCAAGTACAACATATATGGCGAAGCTTTTAATTTTGAGCATTAAGGATAAACAAAATCTCTCTAGGTTGATTTCGTGTATTTTGGGGTGTCGTATATTTTGGATCTAAATGATTGTTCTTTAACAAGGTTGTTAGATCAAATGACAAGAGTTTCAAAACTAATCTGTTCTAATGTGTTCCCTATGACTACAAAATGGTTCAAAAGTGGTTAAACAGTGTTTTG

Coding sequence (CDS)

ATGGAGGAGCTGCTTAAGATCAAGTCCTTTTGCTCTTTCTTCTTGCTTTCTCTTTTGGCCTTCCTCCTTTTACTCCCTCCTTTTGCTGTTGCAGAAACTCACTACCACGAGTTCATTGTGCAACCAAAGCCGGTGAAGAGGCTGTGCAAAGTCCACAACATCATAACAGTGAACGGGAAGTTCCCTGGCCCAACTCTATCGGTCAGAAACGGCGATTCCCTTGTCATCAAAGTAGTGAATGTTGCCCGCTACAATGTCTCTCTCCATTGGCATGGGATCAGGCAGCTGAGGAACCCATGGGCAGATGGGCCAGAGTTCGTGACCCAATGCTCCATAAAGCCAGGAGGCAGCTACACCTACAGGTTCACCATTCAAGACCAAGAGGGAACCCTGTGGTGGCATGCTCATAGCCGATGGCTTCGAGCCACCGTCTATGGCGCTCTCATTATCTACCCCAAGCTCCCCTCTTCATACCCTTTCTTAATGCCCAAAAGAGAAGCCTCTGTTCTTCTTGGGGAATGGTTCGACAGAGACCCGATAAGTGTGCTGCGGCAGGCGCTTTTCACTGGCGCTGCTCCTAATGTCTCTGATGCCTACACCATCAACGGCCAGCCCGGCGACTTCTACCGCTGCTCCGGCAAAGAGACGGTGAGATTCGCGGTGGATTCCGGCGAGACAATTCTTTTGAGGATCATAAACTCAGCATTGAATCAGGAGCTATTCTTCTCCGTCGCGAACCACCAATTGACGGTGGTGGCCGTCGACGCGTCTTACACCAAACCATTCACAACAAATGTGATTATGATCGGACCCGGGCAGACCACCGATGTCCTCCTGACAGCCAACCAACAACCAGCTCACTACTACATGGCTGCCACCGCCTACAACACCGCCCTCAACGCCGCCTTCGACAACACCACCACCACTGCCATCCTCGAATACAAAAACTGCCAGCAACACCAGCAGCAGCCACCGCGACCCACTATGGCTCAGCTCCCAAATTTCAACGACACCCCCACCGCCACCCGATTCACCTCCCAACTCCGGAGCCCCAACAGAGTGAACGTCCCGACCCAAATCGACAACAGCTTACTCTTCACCGTCGGATTAGGCCTCATCAACTGCACGAACCCAAACAGCCCCCGCTGCCAAGGCCCCAACGGCACTCGCTTTGCCGCCAGCATCAACAACGTCTCCTTCGTTTTCCCAAAATCCAATTCCATCATGCAAGCCTTTTACCAGGGCGTTCCGGGGGTTTTCACCACCGACTTTCCGCCGGTTCCGCCGCTGCAATTTGATTACACAGGGAACGTGAGCAGAGGGCTTTGGCAGCCCGGTCGAGGGACCAAGGGTTATAGGCTTAAATATGGATCAAATGTGCAGATTGTGCTGCAGGACACCAGCATTGTAACCACCGAAGACCACCCGATGCATCTCCATGGCTACCATTTCTACGTCGTTGGTTCGGGTTTTGGCAACTTCAACCCTGCGACGGATCCGGCGAGGTTCAATCTCATCGACCCACCGGTGAGAAACACCATTGGAACGCCCCCTGGTGGTTGGGTTGCCATTCGATTCGTGGCTGATAATCCAGGGGCTTGGTTGATGCATTGCCATATAGACTCGCACCTTGCTTGGGGATTGGCGATGGTGTTTCTGGTTGAGAATGGCGAAGGAGAGCTGCAGTCTGTTCTTCCTCCCCCGCCGGATCTGCCTCCTTGTTAA

Protein sequence

MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPISVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVFTTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC
Homology
BLAST of CmaCh13G000340 vs. ExPASy Swiss-Prot
Match: Q56YT0 (Laccase-3 OS=Arabidopsis thaliana OX=3702 GN=LAC3 PE=2 SV=2)

HSP 1 Score: 895.6 bits (2313), Expect = 3.0e-259
Identity = 422/571 (73.91%), Postives = 475/571 (83.19%), Query Frame = 0

Query: 7   IKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTL 66
           ++SF  F LLS +A L      A AE H H+F++ P PVKRLC+ H  ITVNG++PGPTL
Sbjct: 1   MESFRRFSLLSFIALLAYFAFLASAEHHVHQFVITPTPVKRLCRTHQSITVNGQYPGPTL 60

Query: 67  SVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQD 126
            VRNGDSL I V+N ARYN+S+HWHGIRQLRNPWADGPE++TQC I+PG +YTYRF I+D
Sbjct: 61  VVRNGDSLAITVINRARYNISIHWHGIRQLRNPWADGPEYITQCPIRPGQTYTYRFKIED 120

Query: 127 QEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPISVLRQA 186
           QEGTLWWHAHSRWLRATVYGALIIYP+L S YPF MPKR+  +LLGEW+DR+P+ VL+QA
Sbjct: 121 QEGTLWWHAHSRWLRATVYGALIIYPRLGSPYPFSMPKRDIPILLGEWWDRNPMDVLKQA 180

Query: 187 LFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVAN 246
            FTGAA NVSDAYTINGQPGD YRCS   T+RF +  GET+ LR+IN+ +NQELFFSVAN
Sbjct: 181 QFTGAAANVSDAYTINGQPGDLYRCSRAGTIRFPIFPGETVQLRVINAGMNQELFFSVAN 240

Query: 247 HQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNT 306
           HQ TVV  D++YTKPFTTNVIMIGPGQTT+VLLTANQ+P  YYMAA AYN+A NA FDNT
Sbjct: 241 HQFTVVETDSAYTKPFTTNVIMIGPGQTTNVLLTANQRPGRYYMAARAYNSA-NAPFDNT 300

Query: 307 TTTAILEYKNCQQHQ---QQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNS 366
           TTTAIL+Y N    +   +    P    LP FNDT TAT FT++LR   R  VP Q+D +
Sbjct: 301 TTTAILQYVNAPTRRGRGRGQIAPVFPVLPGFNDTATATAFTNRLRYWKRAPVPQQVDEN 360

Query: 367 LLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVFTTD 426
           L FTVGLGLINC NPNSPRCQGPNGTRFAAS+NN+SFV P+SNS+MQA+YQG PG+FTTD
Sbjct: 361 LFFTVGLGLINCANPNSPRCQGPNGTRFAASMNNMSFVLPRSNSVMQAYYQGTPGIFTTD 420

Query: 427 FPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYH 486
           FPPVPP+QFDYTGNVSRGLWQP +GTK Y+LKY SNVQIVLQDTSIVT E+HPMHLHGY 
Sbjct: 421 FPPVPPVQFDYTGNVSRGLWQPIKGTKAYKLKYKSNVQIVLQDTSIVTPENHPMHLHGYQ 480

Query: 487 FYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSH 546
           FYVVGSGFGNFNP TDPARFNL DPP RNTIGTPPGGWVAIRFVADNPGAW MHCHIDSH
Sbjct: 481 FYVVGSGFGNFNPRTDPARFNLFDPPERNTIGTPPGGWVAIRFVADNPGAWFMHCHIDSH 540

Query: 547 LAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           L WGLAMVFLVENG G+LQSV  PP DLP C
Sbjct: 541 LGWGLAMVFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of CmaCh13G000340 vs. ExPASy Swiss-Prot
Match: Q9LYQ2 (Laccase-13 OS=Arabidopsis thaliana OX=3702 GN=LAC13 PE=2 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 2.8e-257
Identity = 426/567 (75.13%), Postives = 478/567 (84.30%), Query Frame = 0

Query: 18  LLAFLLLLPPFAV----AETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTLSVRNGDS 77
           L  F LLL  F      AE H+HEF++Q  PVKRLC+VHN ITVNG+FPGPTL VRNGDS
Sbjct: 4   LRPFFLLLAIFVASLVNAEVHFHEFVIQETPVKRLCRVHNSITVNGQFPGPTLEVRNGDS 63

Query: 78  LVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQDQEGTLWW 137
           LVI  +N ARYN+SLHWHGIRQ+RNPWADGPE++TQC I+PGGSYTYRFT++DQEGTLWW
Sbjct: 64  LVITAINKARYNISLHWHGIRQMRNPWADGPEYITQCPIQPGGSYTYRFTMEDQEGTLWW 123

Query: 138 HAHSRWLRATVYGALIIYPKLPS-SYPF-LMPKREASVLLGEWFDRDPISVLRQALFTGA 197
           HAHSRWLRATVYGALII P L S  YPF ++PKRE ++LLGEW+DR+P+ VL  A FTGA
Sbjct: 124 HAHSRWLRATVYGALIIRPPLSSPHYPFPVIPKREITLLLGEWWDRNPMDVLNLAQFTGA 183

Query: 198 APNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVANHQLTV 257
           APN+SDA+TINGQPGD YRCS +ET+RF V SGE +LLR+INSALNQELFF VANH+LTV
Sbjct: 184 APNISDAFTINGQPGDLYRCSSQETLRFLVGSGEIVLLRVINSALNQELFFGVANHKLTV 243

Query: 258 VAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNTTTTAI 317
           VA DASYTKPF+TNVIM+GPGQTTDVLLTA+Q PAHYYMAA AYN+A NAAFDNTTTTAI
Sbjct: 244 VAADASYTKPFSTNVIMLGPGQTTDVLLTADQPPAHYYMAAHAYNSA-NAAFDNTTTTAI 303

Query: 318 LEYKNCQ---QHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNSLLFTV 377
           L+YK+        +   R   AQLP FNDT TA  FT+Q++SP++V VP +ID +L FTV
Sbjct: 304 LKYKDASCVTLQAKSQARAIPAQLPGFNDTATAAAFTAQMKSPSKVKVPLEIDENLFFTV 363

Query: 378 GLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVP-GVFTTDFPPV 437
           GLGL NC  PN+ RCQGPNGTRF ASINNVSFVFPK NSIMQA+YQG P GVFTTDFPP 
Sbjct: 364 GLGLFNCPTPNTQRCQGPNGTRFTASINNVSFVFPKQNSIMQAYYQGTPTGVFTTDFPPT 423

Query: 438 PPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYHFYVV 497
           PP+ FDYTGNVSRGLWQP RGTK Y+LK+ S VQI+LQDTSIVTTE+HPMHLHGY FYVV
Sbjct: 424 PPVTFDYTGNVSRGLWQPTRGTKAYKLKFNSQVQIILQDTSIVTTENHPMHLHGYEFYVV 483

Query: 498 GSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 557
           G+G GNFNP TD + FNLIDPP RNTIGTPPGGWVAIRFVA+NPGAWLMHCHIDSH+ WG
Sbjct: 484 GTGVGNFNPNTDTSSFNLIDPPRRNTIGTPPGGWVAIRFVANNPGAWLMHCHIDSHIFWG 543

Query: 558 LAMVFLVENGEGELQSVLPPPPDLPPC 575
           LAMVFLVENGEG LQSV  PP DLP C
Sbjct: 544 LAMVFLVENGEGHLQSVQSPPLDLPQC 569

BLAST of CmaCh13G000340 vs. ExPASy Swiss-Prot
Match: Q9SIY8 (Laccase-5 OS=Arabidopsis thaliana OX=3702 GN=LAC5 PE=2 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 1.0e-222
Identity = 361/570 (63.33%), Postives = 435/570 (76.32%), Query Frame = 0

Query: 16  LSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTLSVRNGDSLV 75
           +S +AFLL        + H+HEFI+Q   VKRLC+ HN ITVNG FPGP L V NGD+LV
Sbjct: 11  ISFVAFLLFSSVAEANKAHHHEFIIQATKVKRLCETHNSITVNGMFPGPMLVVNNGDTLV 70

Query: 76  IKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQDQEGTLWWHA 135
           +KV+N ARYN+++HWHG+RQ+R  WADGPEFVTQC I+PG SYTYRFTIQ QEGTLWWHA
Sbjct: 71  VKVINRARYNITIHWHGVRQMRTGWADGPEFVTQCPIRPGSSYTYRFTIQGQEGTLWWHA 130

Query: 136 HSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPISVLRQALFTGAAPNV 195
           HS WLRATVYG+L+++P   SSYPF  P R   +LLGEW+D +P+ VLR+++ TG APN 
Sbjct: 131 HSSWLRATVYGSLLVFPPAGSSYPFTKPHRNVPLLLGEWWDANPVDVLRESIRTGGAPNN 190

Query: 196 SDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVANHQLTVVAVD 255
           SDAYTINGQPGD Y+CS ++T    ++ GETILLR+INSALNQ LFF+VANH+LTVV  D
Sbjct: 191 SDAYTINGQPGDLYKCSSQDTTVVPINVGETILLRVINSALNQPLFFTVANHKLTVVGAD 250

Query: 256 ASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNTTTTAILEYK 315
           ASY KPFTTNVI++GPGQTTDVL+T +Q P  YYMAA AY +A NA F NTTTTAIL+YK
Sbjct: 251 ASYLKPFTTNVIVLGPGQTTDVLITGDQPPNRYYMAARAYQSAQNAPFGNTTTTAILQYK 310

Query: 316 NCQ---------QHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNSLLF 375
           +             +    +P M  LP +NDT T TRF+   RS  R  VPT+ID +L  
Sbjct: 311 SAPCCGVGGGSGTKKGNSFKPIMPILPAYNDTNTVTRFSQSFRSLRRAEVPTEIDENLFV 370

Query: 376 TVGLGLINC-TNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVFTTDFP 435
           T+GLGL NC  N  S RCQGPNGTRF AS+NNVSF  P + S++QA + G+PGVFTTDFP
Sbjct: 371 TIGLGLNNCPKNFRSRRCQGPNGTRFTASMNNVSFALPSNYSLLQAHHHGIPGVFTTDFP 430

Query: 436 PVPPLQFDYTG-NVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYHF 495
             PP++FDYTG N+SR L+QP RGTK Y+LKYGS VQIVLQDT IVT E+HP+HLHGY F
Sbjct: 431 AKPPVKFDYTGNNISRSLYQPDRGTKLYKLKYGSRVQIVLQDTGIVTPENHPIHLHGYDF 490

Query: 496 YVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHL 555
           Y++  GFGNFNP  D A+FNL DPP+RNT+G P  GW  IRF+ADNPG W+MHCH+D+H+
Sbjct: 491 YIIAEGFGNFNPKKDTAKFNLEDPPLRNTVGVPVNGWAVIRFIADNPGVWIMHCHLDAHI 550

Query: 556 AWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           +WGLAM FLVENG G LQ++  PP DLP C
Sbjct: 551 SWGLAMAFLVENGNGVLQTIEQPPHDLPVC 580

BLAST of CmaCh13G000340 vs. ExPASy Swiss-Prot
Match: Q9FLB5 (Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 6.1e-220
Identity = 363/568 (63.91%), Postives = 437/568 (76.94%), Query Frame = 0

Query: 10  FCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTLSVR 69
           FCS F  SL+          +A+  +H+F++Q  PVKRLCK  N ITVNG FPGPTL V 
Sbjct: 13  FCSLFSASLI----------IAKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVN 72

Query: 70  NGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQDQEG 129
           NGD+L +KV N ARYN+++HWHG+RQ+R  WADGPEFVTQC I+PG SYTYRFTIQ QEG
Sbjct: 73  NGDTLEVKVHNRARYNITIHWHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEG 132

Query: 130 TLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPISVLRQALFT 189
           TLWWHAHS WLRATVYGALII+P   SS+PF  P R+ +++LGEW++ +P+ V+ QA  T
Sbjct: 133 TLWWHAHSSWLRATVYGALIIHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRT 192

Query: 190 GAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVANHQL 249
           GAAPN+SDAYTINGQPGD Y CS KETV   ++SGET LLR+IN+ALNQ LFF+VANH+L
Sbjct: 193 GAAPNISDAYTINGQPGDLYNCSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKL 252

Query: 250 TVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNTTTT 309
           TVV  DASY KPFTT V+M+GPGQTTDVLLTA+Q P  YY+AA AY +A NA FDNTTTT
Sbjct: 253 TVVGADASYLKPFTTKVLMLGPGQTTDVLLTADQPPKRYYIAARAYQSAQNAPFDNTTTT 312

Query: 310 AILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNSLLFTVG 369
           AIL+YK          +P M  LP FNDT T T F+ + +S   V VP  ID++L FT+G
Sbjct: 313 AILQYKK----TTTTSKPIMPVLPAFNDTNTVTSFSRKFKSLRNVVVPKTIDDNLFFTIG 372

Query: 370 LGLINCTN--PNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVFTTDFPPV 429
           LGL NC    P S RCQG NGTRF AS+NNVSFV P + S++QA   G+PGVFTTDFP  
Sbjct: 373 LGLDNCPKKFPKS-RCQGLNGTRFTASMNNVSFVLPSNFSLLQAHSNGIPGVFTTDFPSK 432

Query: 430 PPLQFDYTG-NVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYHFYV 489
           PP++FDYTG N+SR L+QP +GTK Y+LKYGS VQ+VLQDT+IVT+E+HP+HLHGY FY+
Sbjct: 433 PPVKFDYTGNNISRALFQPVKGTKLYKLKYGSRVQVVLQDTNIVTSENHPIHLHGYDFYI 492

Query: 490 VGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAW 549
           VG GFGNFNP  D ++FNL+DPP+RNT+  P  GW  IRFVADNPG WLMHCH+D H+ W
Sbjct: 493 VGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKW 552

Query: 550 GLAMVFLVENGEGELQSVLPPPPDLPPC 575
           GLAM FLV+NG GEL+++  PP DLP C
Sbjct: 553 GLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of CmaCh13G000340 vs. ExPASy Swiss-Prot
Match: Q941X2 (Laccase-3 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC3 PE=2 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 1.4e-216
Identity = 358/563 (63.59%), Postives = 424/563 (75.31%), Query Frame = 0

Query: 14  FLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTLSVRNGDS 73
           FLLS     LL    A AE H+HEFIVQ  PVKRLCK HN+ITVNG+ PGPTL VR GD+
Sbjct: 11  FLLSCSVLALL----AGAEVHHHEFIVQETPVKRLCKTHNVITVNGQLPGPTLEVREGDT 70

Query: 74  LVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQDQEGTLWW 133
           +VI VVN A+YNV++HWHGIRQ R  WADGPEFVTQC IKPGGSY YRFTI+ QEGTLWW
Sbjct: 71  VVINVVNHAQYNVTIHWHGIRQFRTGWADGPEFVTQCPIKPGGSYKYRFTIEGQEGTLWW 130

Query: 134 HAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPISVLRQALFTGAAP 193
           HAHS WLRATVYGALII P+   +YPF  P RE  ++LGEW+D DPI V+R+A  TGAAP
Sbjct: 131 HAHSSWLRATVYGALIIRPRENKTYPFEKPAREVPLILGEWWDADPIQVIREAQRTGAAP 190

Query: 194 NVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVANHQLTVVA 253
           N+SDAYTINGQPGD Y CS +ET    V  GET LLR IN+ALNQELF S+A H++TVV 
Sbjct: 191 NISDAYTINGQPGDLYNCSKEETTAVPVKPGETALLRFINAALNQELFVSIAQHKMTVVG 250

Query: 254 VDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNTTTTAILE 313
           VDASYTKPFTT+V+MI PGQTTDVL+T +Q P  YY+AA AY++A   AFDNTTTTA++E
Sbjct: 251 VDASYTKPFTTSVLMIAPGQTTDVLVTMDQAPTRYYLAARAYDSAQGVAFDNTTTTAVIE 310

Query: 314 YK-NCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNSLLFTVGLGL 373
           Y   C         P    LP FNDT TAT F + +RSP+ V +P  +D +L FTVG+GL
Sbjct: 311 YDCGCATDFGPSIPPAFPVLPAFNDTNTATAFAAGIRSPHEVKIPGPVDENLFFTVGVGL 370

Query: 374 INCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVFTTDFPPVPPLQF 433
            NC      +C GPN TRF AS+NN+SFVFP++ S++ A Y G+PGVFTTDFP  PP+QF
Sbjct: 371 FNC--EPGQQCGGPNNTRFTASMNNISFVFPQTTSLLHAHYYGIPGVFTTDFPAYPPVQF 430

Query: 434 DYTG-NVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGF 493
           DYT  NV R LWQP   TK Y+LK+GS VQIVLQDTSIV+ E+HP+H+HGY FY++  GF
Sbjct: 431 DYTAQNVPRYLWQPVPATKLYKLKFGSVVQIVLQDTSIVSPENHPIHIHGYDFYILAEGF 490

Query: 494 GNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWGLAMV 553
           GNF+P  D  +FN +DPP RNT+  P  GW  IRFVADNPG WLMHCH+D H+ WGLAM 
Sbjct: 491 GNFDPKKDAKKFNYVDPPQRNTVAVPTNGWAVIRFVADNPGVWLMHCHLDVHITWGLAMA 550

Query: 554 FLVENGEGELQSVLPPPPDLPPC 575
           FLVE+G G+L+++  PP DLP C
Sbjct: 551 FLVEDGYGKLETLEAPPVDLPMC 567

BLAST of CmaCh13G000340 vs. ExPASy TrEMBL
Match: A0A6J1I651 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111469556 PE=3 SV=1)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 574/574 (100.00%), Postives = 574/574 (100.00%), Query Frame = 0

Query: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60
           MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK
Sbjct: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60

Query: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120
           FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY
Sbjct: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180
           RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI
Sbjct: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180

Query: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240
           SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL
Sbjct: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240

Query: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300
           FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN
Sbjct: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300

Query: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360
           AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI
Sbjct: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360

Query: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420
           DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF
Sbjct: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 574

BLAST of CmaCh13G000340 vs. ExPASy TrEMBL
Match: A0A6J1HPK1 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111464877 PE=3 SV=1)

HSP 1 Score: 1179.5 bits (3050), Expect = 0.0e+00
Identity = 565/574 (98.43%), Postives = 570/574 (99.30%), Query Frame = 0

Query: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60
           MEELLKIKS CSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK
Sbjct: 1   MEELLKIKSCCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60

Query: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120
           FPGPTL+VRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY
Sbjct: 61  FPGPTLAVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180
           RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYP LMPKREASVLLGEWFDRDPI
Sbjct: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPSLMPKREASVLLGEWFDRDPI 180

Query: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240
           SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL
Sbjct: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240

Query: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300
           FFSVANHQLTV+AVDASYTKPFTTNVIM+GPGQTTDVLLTANQQPAHYYMAATAYNTALN
Sbjct: 241 FFSVANHQLTVLAVDASYTKPFTTNVIMVGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300

Query: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360
           AAFDNTTTTAILEYKNCQQHQQQPPRP MAQLPNFNDTPTATRFTSQLRSPNRVNVPT+I
Sbjct: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPIMAQLPNFNDTPTATRFTSQLRSPNRVNVPTRI 360

Query: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420
           D+SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF
Sbjct: 361 DDSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTK YRLKYGSNVQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKAYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 574

BLAST of CmaCh13G000340 vs. ExPASy TrEMBL
Match: A0A6J1K8J5 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111493234 PE=3 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 9.4e-309
Identity = 513/574 (89.37%), Postives = 537/574 (93.55%), Query Frame = 0

Query: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60
           ME +LK KS  S FLL+    LL+L PFA+AETHYHEFIVQ KPVKRLCKVHNIITVNG+
Sbjct: 1   MEHMLKTKSLSSCFLLA----LLVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQ 60

Query: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120
           FPGPTL VRNGDSLVIKVVN  RYNVSLHWHGI+QLRNPWADGPEFVTQCSIKPGGSYTY
Sbjct: 61  FPGPTLEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180
           RFTI+DQEGTLWWHAHSRWLRATVYGALIIYPK+ S YPFLMPKREASVLLGEWFDRDP+
Sbjct: 121 RFTIEDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPM 180

Query: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240
           SVLRQALF+GAAPNVSDAYTINGQPGDFYRCS KETVRF V+SGETILLRI+NS LNQEL
Sbjct: 181 SVLRQALFSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNSGETILLRIVNSGLNQEL 240

Query: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300
           FFSVANH LTVVAVDASYTKPF TNVIMIGPGQTTDVLLTANQ PAHYYMAATAYNTA+N
Sbjct: 241 FFSVANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMN 300

Query: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360
           AAFDNTTTTAIL+Y N  Q QQ   +P +AQLP FNDTPT+TRFTSQLRS NR NVPTQI
Sbjct: 301 AAFDNTTTTAILQYNNNYQQQQ---KPILAQLPFFNDTPTSTRFTSQLRSLNRANVPTQI 360

Query: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420
           D SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINN SFVFPKSNSIMQAFYQG+PGVF
Sbjct: 361 DESLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTK +RL+YGS+VQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFN ATDPARFNL+DPP+RNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNSATDPARFNLVDPPLRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           DSHLAWGLAMVFLVENGEGE+QSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 567

BLAST of CmaCh13G000340 vs. ExPASy TrEMBL
Match: A0A6J1G957 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111452103 PE=3 SV=1)

HSP 1 Score: 1061.6 bits (2744), Expect = 1.2e-306
Identity = 507/574 (88.33%), Postives = 536/574 (93.38%), Query Frame = 0

Query: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60
           ME LLK KS  S FLL+    +L+L PFA+AETHYHEFIVQ KPVKRLCKVHNIITVNG+
Sbjct: 1   MEHLLKTKSLSSCFLLA----ILVLIPFAIAETHYHEFIVQRKPVKRLCKVHNIITVNGQ 60

Query: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120
           FPGP L VRNGDSLVIKVVN  RYNVSLHWHGI+QLRNPWADGPEF+TQCSIKPGGSYTY
Sbjct: 61  FPGPILEVRNGDSLVIKVVNAVRYNVSLHWHGIKQLRNPWADGPEFITQCSIKPGGSYTY 120

Query: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180
           RFTI+DQEGTLWWHAHSRWLRATVYGALIIYPK+ S YPFLMPKREASVLLGEWFDRDP+
Sbjct: 121 RFTIEDQEGTLWWHAHSRWLRATVYGALIIYPKMGSYYPFLMPKREASVLLGEWFDRDPM 180

Query: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240
           SVLRQAL +GAAPNVSDAYTINGQPGDFYRCS KETVRF V++GETILLRI+NS LNQEL
Sbjct: 181 SVLRQALLSGAAPNVSDAYTINGQPGDFYRCSKKETVRFGVNAGETILLRIVNSGLNQEL 240

Query: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300
           FFSVANH LTVVAVDASYTKPF TNVIMIGPGQTTDVLLTANQ PAHYYMAATAYNTA+N
Sbjct: 241 FFSVANHPLTVVAVDASYTKPFKTNVIMIGPGQTTDVLLTANQPPAHYYMAATAYNTAMN 300

Query: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360
           A+FDNTTTTAIL+Y N   + QQ P+P +AQLP FNDTPT+TRFT+QLRS NR NVPTQI
Sbjct: 301 ASFDNTTTTAILQYNN---NYQQQPKPILAQLPFFNDTPTSTRFTAQLRSLNRANVPTQI 360

Query: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420
           D SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINN SFVFPKSNSIMQAFYQG+PGVF
Sbjct: 361 DESLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNNSFVFPKSNSIMQAFYQGIPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTK +RL+YGS+VQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKTFRLRYGSSVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFN ATDPARFNL+DPP+RNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNSATDPARFNLVDPPMRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           DSHLAWGLAMVFLVENGEGE+QSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGEMQSVLPPPPDLPPC 567

BLAST of CmaCh13G000340 vs. ExPASy TrEMBL
Match: A0A5D3BDS8 (Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00710 PE=3 SV=1)

HSP 1 Score: 1038.5 bits (2684), Expect = 1.0e-299
Identity = 497/576 (86.28%), Postives = 530/576 (92.01%), Query Frame = 0

Query: 1   MEELLKIK-SFCSFFLL-SLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVN 60
           MEE + +K S CSFFLL SLL FL LL  F + +THYH+FIVQPKPVKRLCKVHNIITVN
Sbjct: 1   MEEFVNLKSSICSFFLLISLLGFLPLLSAFPLPKTHYHQFIVQPKPVKRLCKVHNIITVN 60

Query: 61  GKFPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSY 120
           G+FPGPTL+VR+GDSLVIKVVN ARYNVSLHWHGIRQLRNPWADGPEF+TQCSIKPGGSY
Sbjct: 61  GQFPGPTLAVRDGDSLVIKVVNAARYNVSLHWHGIRQLRNPWADGPEFITQCSIKPGGSY 120

Query: 121 TYRFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRD 180
           TYRFTI+ QEGTLWWHAHSRWLRATVYGALIIYPKL S +PF+MPK+E  +LLGEWFDRD
Sbjct: 121 TYRFTIEGQEGTLWWHAHSRWLRATVYGALIIYPKLASPHPFVMPKKEFPLLLGEWFDRD 180

Query: 181 PISVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQ 240
           PISVLRQALFTGAAPNVSDAYTINGQPGDFY CS KET+R AV+SGETILLRIINSALNQ
Sbjct: 181 PISVLRQALFTGAAPNVSDAYTINGQPGDFYICSKKETMRLAVNSGETILLRIINSALNQ 240

Query: 241 ELFFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTA 300
           ELFFS+ANHQ+TVVAVDA+YTKPF TNVIMIGPGQTTDVL+TANQ PA+YYMAATAYNTA
Sbjct: 241 ELFFSIANHQMTVVAVDAAYTKPFATNVIMIGPGQTTDVLVTANQPPAYYYMAATAYNTA 300

Query: 301 LNAAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPT 360
            NA FDNTTTTAIL+Y N     QQ PRP +AQLPNFNDTPTATRFT QLRSP+RV+VP 
Sbjct: 301 QNAPFDNTTTTAILQYNNLP--PQQNPRPILAQLPNFNDTPTATRFTDQLRSPHRVSVPL 360

Query: 361 QIDNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPG 420
            ID +L FTVGLGL NCTNPNSPRCQGPNGTRF ASINNVSFVFPKSNSIMQA+YQGVPG
Sbjct: 361 HIDENLFFTVGLGLNNCTNPNSPRCQGPNGTRFTASINNVSFVFPKSNSIMQAYYQGVPG 420

Query: 421 VFTTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMH 480
           VFT DFPP PPLQFDYTGNVSRGLWQP RGTK Y+LKYGS+VQIVLQDTSIVT E+HPMH
Sbjct: 421 VFTADFPPFPPLQFDYTGNVSRGLWQPRRGTKAYKLKYGSSVQIVLQDTSIVTPENHPMH 480

Query: 481 LHGYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHC 540
           LHGYHFYVVGSGFGNFNP TDPARFNLIDPPVRNTIGTP GGWVAIRF+ADNPGAWLMHC
Sbjct: 481 LHGYHFYVVGSGFGNFNPRTDPARFNLIDPPVRNTIGTPTGGWVAIRFIADNPGAWLMHC 540

Query: 541 HIDSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           HIDSHLAWGLAMVFLVENGEGE+QSV+PPPPDLPPC
Sbjct: 541 HIDSHLAWGLAMVFLVENGEGEMQSVIPPPPDLPPC 574

BLAST of CmaCh13G000340 vs. NCBI nr
Match: XP_022970624.1 (laccase-13-like [Cucurbita maxima])

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 574/574 (100.00%), Postives = 574/574 (100.00%), Query Frame = 0

Query: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60
           MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK
Sbjct: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60

Query: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120
           FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY
Sbjct: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180
           RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI
Sbjct: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180

Query: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240
           SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL
Sbjct: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240

Query: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300
           FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN
Sbjct: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300

Query: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360
           AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI
Sbjct: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360

Query: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420
           DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF
Sbjct: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 574

BLAST of CmaCh13G000340 vs. NCBI nr
Match: XP_023520100.1 (laccase-13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1183.7 bits (3061), Expect = 0.0e+00
Identity = 568/574 (98.95%), Postives = 570/574 (99.30%), Query Frame = 0

Query: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60
           MEELLKIKS CS FLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK
Sbjct: 1   MEELLKIKSCCSLFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60

Query: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120
           FPGPTL+VRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY
Sbjct: 61  FPGPTLAVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180
           RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI
Sbjct: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180

Query: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240
           SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL
Sbjct: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240

Query: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300
           FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN
Sbjct: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300

Query: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360
           AAFDNTTTTAILEYKNCQQHQQ PPRP MAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI
Sbjct: 301 AAFDNTTTTAILEYKNCQQHQQHPPRPIMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360

Query: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420
           D+SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF
Sbjct: 361 DDSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 574

BLAST of CmaCh13G000340 vs. NCBI nr
Match: XP_022964914.1 (laccase-13-like [Cucurbita moschata])

HSP 1 Score: 1179.5 bits (3050), Expect = 0.0e+00
Identity = 565/574 (98.43%), Postives = 570/574 (99.30%), Query Frame = 0

Query: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60
           MEELLKIKS CSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK
Sbjct: 1   MEELLKIKSCCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60

Query: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120
           FPGPTL+VRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY
Sbjct: 61  FPGPTLAVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180
           RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYP LMPKREASVLLGEWFDRDPI
Sbjct: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPSLMPKREASVLLGEWFDRDPI 180

Query: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240
           SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL
Sbjct: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQEL 240

Query: 241 FFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300
           FFSVANHQLTV+AVDASYTKPFTTNVIM+GPGQTTDVLLTANQQPAHYYMAATAYNTALN
Sbjct: 241 FFSVANHQLTVLAVDASYTKPFTTNVIMVGPGQTTDVLLTANQQPAHYYMAATAYNTALN 300

Query: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQI 360
           AAFDNTTTTAILEYKNCQQHQQQPPRP MAQLPNFNDTPTATRFTSQLRSPNRVNVPT+I
Sbjct: 301 AAFDNTTTTAILEYKNCQQHQQQPPRPIMAQLPNFNDTPTATRFTSQLRSPNRVNVPTRI 360

Query: 361 DNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420
           D+SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF
Sbjct: 361 DDSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVF 420

Query: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480
           TTDFPPVPPLQFDYTGNVSRGLWQPGRGTK YRLKYGSNVQIVLQDTSIVTTEDHPMHLH
Sbjct: 421 TTDFPPVPPLQFDYTGNVSRGLWQPGRGTKAYRLKYGSNVQIVLQDTSIVTTEDHPMHLH 480

Query: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540
           GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI
Sbjct: 481 GYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHI 540

Query: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC
Sbjct: 541 DSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 574

BLAST of CmaCh13G000340 vs. NCBI nr
Match: KAG7019107.1 (Laccase-3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 528/535 (98.69%), Postives = 532/535 (99.44%), Query Frame = 0

Query: 40  VQPKPVKRLCKVHNIITVNGKFPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNP 99
           VQPKPVKRLCKVHNIITVNGKFPGPTL+VRNGDSLVIKVVNVARYNVSLHWHGIRQLRNP
Sbjct: 4   VQPKPVKRLCKVHNIITVNGKFPGPTLAVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNP 63

Query: 100 WADGPEFVTQCSIKPGGSYTYRFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYP 159
           WADGPEFVTQCSIKPGGSYTYRFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYP
Sbjct: 64  WADGPEFVTQCSIKPGGSYTYRFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYP 123

Query: 160 FLMPKREASVLLGEWFDRDPISVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRF 219
           FLMPKREASVLLGEWFDRDPISVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRF
Sbjct: 124 FLMPKREASVLLGEWFDRDPISVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRF 183

Query: 220 AVDSGETILLRIINSALNQELFFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLL 279
           AVDSGETILLR+INSALNQELFFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLL
Sbjct: 184 AVDSGETILLRVINSALNQELFFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLL 243

Query: 280 TANQQPAHYYMAATAYNTALNAAFDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTP 339
           TANQQPAHYYMAATAYNTALNAAFDNTTTTAILEYKNCQQHQQQPPRP MAQLPNFNDTP
Sbjct: 244 TANQQPAHYYMAATAYNTALNAAFDNTTTTAILEYKNCQQHQQQPPRPIMAQLPNFNDTP 303

Query: 340 TATRFTSQLRSPNRVNVPTQIDNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVS 399
           TATRFTSQLRSPNRVNVPT+ID+SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVS
Sbjct: 304 TATRFTSQLRSPNRVNVPTRIDDSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVS 363

Query: 400 FVFPKSNSIMQAFYQGVPGVFTTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSN 459
           FVFPKSNSIMQAFYQGVPGVFTTDFPPVPPLQFDYTGNVSRGLWQPGRGTK Y LKYGSN
Sbjct: 364 FVFPKSNSIMQAFYQGVPGVFTTDFPPVPPLQFDYTGNVSRGLWQPGRGTKAYTLKYGSN 423

Query: 460 VQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPG 519
           VQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPG
Sbjct: 424 VQIVLQDTSIVTTEDHPMHLHGYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPG 483

Query: 520 GWVAIRFVADNPGAWLMHCHIDSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           GWVAIRFVADNPGAWLMHCHIDSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC
Sbjct: 484 GWVAIRFVADNPGAWLMHCHIDSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 538

BLAST of CmaCh13G000340 vs. NCBI nr
Match: XP_038895520.1 (laccase-13-like [Benincasa hispida])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.3e-309
Identity = 509/576 (88.37%), Postives = 541/576 (93.92%), Query Frame = 0

Query: 1   MEELLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGK 60
           MEEL  +KSFCSFFL+SLL F  LL  FAVAETHYHEFIVQPK VKRLCKVHN+ITVNG+
Sbjct: 1   MEELFNMKSFCSFFLVSLLGFFPLLSTFAVAETHYHEFIVQPKAVKRLCKVHNVITVNGQ 60

Query: 61  FPGPTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120
           FPGPTL+VR+GDSLVIKVVN ARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY
Sbjct: 61  FPGPTLAVRDGDSLVIKVVNAARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTY 120

Query: 121 RFTIQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI 180
           RFTI+DQEGTLWWHAHSRWLRATVYGALIIYPKL S YPF+MP+RE  +LLGEWFDRDPI
Sbjct: 121 RFTIEDQEGTLWWHAHSRWLRATVYGALIIYPKLGSPYPFIMPEREVPLLLGEWFDRDPI 180

Query: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYRCSGK-ETVRFAVDSGETILLRIINSALNQE 240
           SVLRQALFTGAAPNVSDAYTINGQPGDFY CS K ETVR AVDSGETILLRIINSALNQE
Sbjct: 181 SVLRQALFTGAAPNVSDAYTINGQPGDFYICSKKVETVRLAVDSGETILLRIINSALNQE 240

Query: 241 LFFSVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTAL 300
           LFFS+ANHQ+TVVAVDA+YTKPF TNVIMIGPGQTTDVL+TANQ PAHYYMAATAYNTA+
Sbjct: 241 LFFSIANHQMTVVAVDAAYTKPFATNVIMIGPGQTTDVLITANQPPAHYYMAATAYNTAI 300

Query: 301 NAAFDNTTTTAILEYKNC-QQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPT 360
           NAAFDNTTTTAIL+Y NC QQ ++Q  +P +AQLPNFNDTPTATRFT QLRSPN+VNVPT
Sbjct: 301 NAAFDNTTTTAILQYNNCPQQQKKQQSQPILAQLPNFNDTPTATRFTDQLRSPNQVNVPT 360

Query: 361 QIDNSLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPG 420
           QID +L FTVGLGL NCTNPNSPRCQGPNGTRF ASINNVSFVFPKSNSIMQA+YQGVPG
Sbjct: 361 QIDENLFFTVGLGLNNCTNPNSPRCQGPNGTRFTASINNVSFVFPKSNSIMQAYYQGVPG 420

Query: 421 VFTTDFPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMH 480
           VFT DFPPVPPLQFDYTGNVSRGLWQP RGTK YRL+YGS+VQIVLQDT+IVTTE+HPMH
Sbjct: 421 VFTADFPPVPPLQFDYTGNVSRGLWQPSRGTKAYRLRYGSSVQIVLQDTNIVTTENHPMH 480

Query: 481 LHGYHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHC 540
           LHGYHFYVVGSGFGNFNPATDP+RFNL+DPPVRNTIGTP GGWVAIRF+ADNPGAWLMHC
Sbjct: 481 LHGYHFYVVGSGFGNFNPATDPSRFNLVDPPVRNTIGTPTGGWVAIRFIADNPGAWLMHC 540

Query: 541 HIDSHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           HIDSHLAWGLAMVFLVENG+GE+QSVLPPP DLPPC
Sbjct: 541 HIDSHLAWGLAMVFLVENGQGEMQSVLPPPLDLPPC 576

BLAST of CmaCh13G000340 vs. TAIR 10
Match: AT2G30210.1 (laccase 3 )

HSP 1 Score: 895.6 bits (2313), Expect = 2.1e-260
Identity = 422/571 (73.91%), Postives = 475/571 (83.19%), Query Frame = 0

Query: 7   IKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTL 66
           ++SF  F LLS +A L      A AE H H+F++ P PVKRLC+ H  ITVNG++PGPTL
Sbjct: 1   MESFRRFSLLSFIALLAYFAFLASAEHHVHQFVITPTPVKRLCRTHQSITVNGQYPGPTL 60

Query: 67  SVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQD 126
            VRNGDSL I V+N ARYN+S+HWHGIRQLRNPWADGPE++TQC I+PG +YTYRF I+D
Sbjct: 61  VVRNGDSLAITVINRARYNISIHWHGIRQLRNPWADGPEYITQCPIRPGQTYTYRFKIED 120

Query: 127 QEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPISVLRQA 186
           QEGTLWWHAHSRWLRATVYGALIIYP+L S YPF MPKR+  +LLGEW+DR+P+ VL+QA
Sbjct: 121 QEGTLWWHAHSRWLRATVYGALIIYPRLGSPYPFSMPKRDIPILLGEWWDRNPMDVLKQA 180

Query: 187 LFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVAN 246
            FTGAA NVSDAYTINGQPGD YRCS   T+RF +  GET+ LR+IN+ +NQELFFSVAN
Sbjct: 181 QFTGAAANVSDAYTINGQPGDLYRCSRAGTIRFPIFPGETVQLRVINAGMNQELFFSVAN 240

Query: 247 HQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNT 306
           HQ TVV  D++YTKPFTTNVIMIGPGQTT+VLLTANQ+P  YYMAA AYN+A NA FDNT
Sbjct: 241 HQFTVVETDSAYTKPFTTNVIMIGPGQTTNVLLTANQRPGRYYMAARAYNSA-NAPFDNT 300

Query: 307 TTTAILEYKNCQQHQ---QQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNS 366
           TTTAIL+Y N    +   +    P    LP FNDT TAT FT++LR   R  VP Q+D +
Sbjct: 301 TTTAILQYVNAPTRRGRGRGQIAPVFPVLPGFNDTATATAFTNRLRYWKRAPVPQQVDEN 360

Query: 367 LLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVFTTD 426
           L FTVGLGLINC NPNSPRCQGPNGTRFAAS+NN+SFV P+SNS+MQA+YQG PG+FTTD
Sbjct: 361 LFFTVGLGLINCANPNSPRCQGPNGTRFAASMNNMSFVLPRSNSVMQAYYQGTPGIFTTD 420

Query: 427 FPPVPPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYH 486
           FPPVPP+QFDYTGNVSRGLWQP +GTK Y+LKY SNVQIVLQDTSIVT E+HPMHLHGY 
Sbjct: 421 FPPVPPVQFDYTGNVSRGLWQPIKGTKAYKLKYKSNVQIVLQDTSIVTPENHPMHLHGYQ 480

Query: 487 FYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSH 546
           FYVVGSGFGNFNP TDPARFNL DPP RNTIGTPPGGWVAIRFVADNPGAW MHCHIDSH
Sbjct: 481 FYVVGSGFGNFNPRTDPARFNLFDPPERNTIGTPPGGWVAIRFVADNPGAWFMHCHIDSH 540

Query: 547 LAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           L WGLAMVFLVENG G+LQSV  PP DLP C
Sbjct: 541 LGWGLAMVFLVENGRGQLQSVQAPPLDLPRC 570

BLAST of CmaCh13G000340 vs. TAIR 10
Match: AT5G07130.1 (laccase 13 )

HSP 1 Score: 889.0 bits (2296), Expect = 2.0e-258
Identity = 426/567 (75.13%), Postives = 478/567 (84.30%), Query Frame = 0

Query: 18  LLAFLLLLPPFAV----AETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTLSVRNGDS 77
           L  F LLL  F      AE H+HEF++Q  PVKRLC+VHN ITVNG+FPGPTL VRNGDS
Sbjct: 4   LRPFFLLLAIFVASLVNAEVHFHEFVIQETPVKRLCRVHNSITVNGQFPGPTLEVRNGDS 63

Query: 78  LVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQDQEGTLWW 137
           LVI  +N ARYN+SLHWHGIRQ+RNPWADGPE++TQC I+PGGSYTYRFT++DQEGTLWW
Sbjct: 64  LVITAINKARYNISLHWHGIRQMRNPWADGPEYITQCPIQPGGSYTYRFTMEDQEGTLWW 123

Query: 138 HAHSRWLRATVYGALIIYPKLPS-SYPF-LMPKREASVLLGEWFDRDPISVLRQALFTGA 197
           HAHSRWLRATVYGALII P L S  YPF ++PKRE ++LLGEW+DR+P+ VL  A FTGA
Sbjct: 124 HAHSRWLRATVYGALIIRPPLSSPHYPFPVIPKREITLLLGEWWDRNPMDVLNLAQFTGA 183

Query: 198 APNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVANHQLTV 257
           APN+SDA+TINGQPGD YRCS +ET+RF V SGE +LLR+INSALNQELFF VANH+LTV
Sbjct: 184 APNISDAFTINGQPGDLYRCSSQETLRFLVGSGEIVLLRVINSALNQELFFGVANHKLTV 243

Query: 258 VAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNTTTTAI 317
           VA DASYTKPF+TNVIM+GPGQTTDVLLTA+Q PAHYYMAA AYN+A NAAFDNTTTTAI
Sbjct: 244 VAADASYTKPFSTNVIMLGPGQTTDVLLTADQPPAHYYMAAHAYNSA-NAAFDNTTTTAI 303

Query: 318 LEYKNCQ---QHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNSLLFTV 377
           L+YK+        +   R   AQLP FNDT TA  FT+Q++SP++V VP +ID +L FTV
Sbjct: 304 LKYKDASCVTLQAKSQARAIPAQLPGFNDTATAAAFTAQMKSPSKVKVPLEIDENLFFTV 363

Query: 378 GLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVP-GVFTTDFPPV 437
           GLGL NC  PN+ RCQGPNGTRF ASINNVSFVFPK NSIMQA+YQG P GVFTTDFPP 
Sbjct: 364 GLGLFNCPTPNTQRCQGPNGTRFTASINNVSFVFPKQNSIMQAYYQGTPTGVFTTDFPPT 423

Query: 438 PPLQFDYTGNVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYHFYVV 497
           PP+ FDYTGNVSRGLWQP RGTK Y+LK+ S VQI+LQDTSIVTTE+HPMHLHGY FYVV
Sbjct: 424 PPVTFDYTGNVSRGLWQPTRGTKAYKLKFNSQVQIILQDTSIVTTENHPMHLHGYEFYVV 483

Query: 498 GSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAWG 557
           G+G GNFNP TD + FNLIDPP RNTIGTPPGGWVAIRFVA+NPGAWLMHCHIDSH+ WG
Sbjct: 484 GTGVGNFNPNTDTSSFNLIDPPRRNTIGTPPGGWVAIRFVANNPGAWLMHCHIDSHIFWG 543

Query: 558 LAMVFLVENGEGELQSVLPPPPDLPPC 575
           LAMVFLVENGEG LQSV  PP DLP C
Sbjct: 544 LAMVFLVENGEGHLQSVQSPPLDLPQC 569

BLAST of CmaCh13G000340 vs. TAIR 10
Match: AT2G40370.1 (laccase 5 )

HSP 1 Score: 774.2 bits (1998), Expect = 7.1e-224
Identity = 361/570 (63.33%), Postives = 435/570 (76.32%), Query Frame = 0

Query: 16  LSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTLSVRNGDSLV 75
           +S +AFLL        + H+HEFI+Q   VKRLC+ HN ITVNG FPGP L V NGD+LV
Sbjct: 11  ISFVAFLLFSSVAEANKAHHHEFIIQATKVKRLCETHNSITVNGMFPGPMLVVNNGDTLV 70

Query: 76  IKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQDQEGTLWWHA 135
           +KV+N ARYN+++HWHG+RQ+R  WADGPEFVTQC I+PG SYTYRFTIQ QEGTLWWHA
Sbjct: 71  VKVINRARYNITIHWHGVRQMRTGWADGPEFVTQCPIRPGSSYTYRFTIQGQEGTLWWHA 130

Query: 136 HSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPISVLRQALFTGAAPNV 195
           HS WLRATVYG+L+++P   SSYPF  P R   +LLGEW+D +P+ VLR+++ TG APN 
Sbjct: 131 HSSWLRATVYGSLLVFPPAGSSYPFTKPHRNVPLLLGEWWDANPVDVLRESIRTGGAPNN 190

Query: 196 SDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVANHQLTVVAVD 255
           SDAYTINGQPGD Y+CS ++T    ++ GETILLR+INSALNQ LFF+VANH+LTVV  D
Sbjct: 191 SDAYTINGQPGDLYKCSSQDTTVVPINVGETILLRVINSALNQPLFFTVANHKLTVVGAD 250

Query: 256 ASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNTTTTAILEYK 315
           ASY KPFTTNVI++GPGQTTDVL+T +Q P  YYMAA AY +A NA F NTTTTAIL+YK
Sbjct: 251 ASYLKPFTTNVIVLGPGQTTDVLITGDQPPNRYYMAARAYQSAQNAPFGNTTTTAILQYK 310

Query: 316 NCQ---------QHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNSLLF 375
           +             +    +P M  LP +NDT T TRF+   RS  R  VPT+ID +L  
Sbjct: 311 SAPCCGVGGGSGTKKGNSFKPIMPILPAYNDTNTVTRFSQSFRSLRRAEVPTEIDENLFV 370

Query: 376 TVGLGLINC-TNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVFTTDFP 435
           T+GLGL NC  N  S RCQGPNGTRF AS+NNVSF  P + S++QA + G+PGVFTTDFP
Sbjct: 371 TIGLGLNNCPKNFRSRRCQGPNGTRFTASMNNVSFALPSNYSLLQAHHHGIPGVFTTDFP 430

Query: 436 PVPPLQFDYTG-NVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYHF 495
             PP++FDYTG N+SR L+QP RGTK Y+LKYGS VQIVLQDT IVT E+HP+HLHGY F
Sbjct: 431 AKPPVKFDYTGNNISRSLYQPDRGTKLYKLKYGSRVQIVLQDTGIVTPENHPIHLHGYDF 490

Query: 496 YVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHL 555
           Y++  GFGNFNP  D A+FNL DPP+RNT+G P  GW  IRF+ADNPG W+MHCH+D+H+
Sbjct: 491 YIIAEGFGNFNPKKDTAKFNLEDPPLRNTVGVPVNGWAVIRFIADNPGVWIMHCHLDAHI 550

Query: 556 AWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
           +WGLAM FLVENG G LQ++  PP DLP C
Sbjct: 551 SWGLAMAFLVENGNGVLQTIEQPPHDLPVC 580

BLAST of CmaCh13G000340 vs. TAIR 10
Match: AT5G05390.1 (laccase 12 )

HSP 1 Score: 765.0 bits (1974), Expect = 4.3e-221
Identity = 363/568 (63.91%), Postives = 437/568 (76.94%), Query Frame = 0

Query: 10  FCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGKFPGPTLSVR 69
           FCS F  SL+          +A+  +H+F++Q  PVKRLCK  N ITVNG FPGPTL V 
Sbjct: 13  FCSLFSASLI----------IAKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPTLEVN 72

Query: 70  NGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFTIQDQEG 129
           NGD+L +KV N ARYN+++HWHG+RQ+R  WADGPEFVTQC I+PG SYTYRFTIQ QEG
Sbjct: 73  NGDTLEVKVHNRARYNITIHWHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEG 132

Query: 130 TLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPISVLRQALFT 189
           TLWWHAHS WLRATVYGALII+P   SS+PF  P R+ +++LGEW++ +P+ V+ QA  T
Sbjct: 133 TLWWHAHSSWLRATVYGALIIHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRT 192

Query: 190 GAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFFSVANHQL 249
           GAAPN+SDAYTINGQPGD Y CS KETV   ++SGET LLR+IN+ALNQ LFF+VANH+L
Sbjct: 193 GAAPNISDAYTINGQPGDLYNCSTKETVVVPINSGETSLLRVINAALNQPLFFTVANHKL 252

Query: 250 TVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAAFDNTTTT 309
           TVV  DASY KPFTT V+M+GPGQTTDVLLTA+Q P  YY+AA AY +A NA FDNTTTT
Sbjct: 253 TVVGADASYLKPFTTKVLMLGPGQTTDVLLTADQPPKRYYIAARAYQSAQNAPFDNTTTT 312

Query: 310 AILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDNSLLFTVG 369
           AIL+YK          +P M  LP FNDT T T F+ + +S   V VP  ID++L FT+G
Sbjct: 313 AILQYKK----TTTTSKPIMPVLPAFNDTNTVTSFSRKFKSLRNVVVPKTIDDNLFFTIG 372

Query: 370 LGLINCTN--PNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVFTTDFPPV 429
           LGL NC    P S RCQG NGTRF AS+NNVSFV P + S++QA   G+PGVFTTDFP  
Sbjct: 373 LGLDNCPKKFPKS-RCQGLNGTRFTASMNNVSFVLPSNFSLLQAHSNGIPGVFTTDFPSK 432

Query: 430 PPLQFDYTG-NVSRGLWQPGRGTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHGYHFYV 489
           PP++FDYTG N+SR L+QP +GTK Y+LKYGS VQ+VLQDT+IVT+E+HP+HLHGY FY+
Sbjct: 433 PPVKFDYTGNNISRALFQPVKGTKLYKLKYGSRVQVVLQDTNIVTSENHPIHLHGYDFYI 492

Query: 490 VGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHIDSHLAW 549
           VG GFGNFNP  D ++FNL+DPP+RNT+  P  GW  IRFVADNPG WLMHCH+D H+ W
Sbjct: 493 VGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKW 552

Query: 550 GLAMVFLVENGEGELQSVLPPPPDLPPC 575
           GLAM FLV+NG GEL+++  PP DLP C
Sbjct: 553 GLAMAFLVDNGVGELETLEAPPHDLPIC 565

BLAST of CmaCh13G000340 vs. TAIR 10
Match: AT5G09360.1 (laccase 14 )

HSP 1 Score: 609.0 bits (1569), Expect = 4.0e-174
Identity = 296/573 (51.66%), Postives = 395/573 (68.94%), Query Frame = 0

Query: 4   LLKIKSFCSFFLLSLLAFLLLLPPFAVAETHYHEFIVQPKPVKRLCKVHNIITVNGKFPG 63
           ++K      FFL  LLAF +     A AE H+H F ++ K   RLC  + I+TVNG+FPG
Sbjct: 11  IIKTLQTIVFFLFVLLAFQI-----AEAEIHHHTFKIKSKAYTRLCNTNKILTVNGEFPG 70

Query: 64  PTLSVRNGDSLVIKVVNVARYNVSLHWHGIRQLRNPWADGPEFVTQCSIKPGGSYTYRFT 123
           PTL    GD L++ V+N A YN++LHWHG RQ+RNPW+DGPE+VTQC I+PG SY YR  
Sbjct: 71  PTLKAYRGDKLIVNVINNANYNITLHWHGARQIRNPWSDGPEYVTQCPIRPGESYVYRID 130

Query: 124 IQDQEGTLWWHAHSRWLRATVYGALIIYPKLPSSYPFLMPKREASVLLGEWFDRDPI-SV 183
           ++ +EGT+WWHAHS+W RATV+GA I+YPK  SSYPF  P RE  ++LGEW+ ++ I  +
Sbjct: 131 LKVEEGTIWWHAHSQWARATVHGAFIVYPKRGSSYPFPKPHREIPLILGEWWKKENIMHI 190

Query: 184 LRQALFTGAAPNVSDAYTINGQPGDFYRCSGKETVRFAVDSGETILLRIINSALNQELFF 243
             +A  TG  P +SD+YTINGQPG  Y CS  ET +  V  G   LLRIIN+ +++ELFF
Sbjct: 191 PGKANKTGGEPAISDSYTINGQPGYLYPCSKPETFKITVVRGRRYLLRIINAVMDEELFF 250

Query: 244 SVANHQLTVVAVDASYTKPFTTNVIMIGPGQTTDVLLTANQQPAHYYMAATAYNTALNAA 303
           ++ANH LTVVA D  Y K F ++ +MI PGQ+ DVLL ANQ+P HY++AA AY++A  A 
Sbjct: 251 AIANHTLTVVAKDGFYLKHFKSDYLMITPGQSMDVLLHANQRPNHYFVAARAYSSAFGAG 310

Query: 304 FDNTTTTAILEYKNCQQHQQQPPRPTMAQLPNFNDTPTATRFTSQLRSPNRVNVPTQIDN 363
           FD TTTTAIL+YK    ++ +P  P    LP +N T  +TRFT+Q RS   VNVP +I+ 
Sbjct: 311 FDKTTTTAILQYKGDTLNRIKPILP---YLPPYNRTEASTRFTNQFRSQRPVNVPVKINT 370

Query: 364 SLLFTVGLGLINCTNPNSPRCQGPNGTRFAASINNVSFVFPKSNSIMQAFYQGVPGVFTT 423
            LL+ + + L+NC++     C GP G RF++SINN+SFV P S  I++A+Y+ + GVF  
Sbjct: 371 RLLYAISVNLMNCSDDRP--CTGPFGKRFSSSINNISFVNP-SVDILRAYYRHIGGVFQE 430

Query: 424 DFPPVPPLQFDYTGNVSRGLWQPGR-GTKGYRLKYGSNVQIVLQDTSIVTTEDHPMHLHG 483
           DFP  PP +F+YTG     L  P R GTK   L Y S+V+++LQ T++  +  HP+HLHG
Sbjct: 431 DFPRNPPTKFNYTG---ENLPFPTRFGTKVVVLDYNSSVELILQGTTVWASNIHPIHLHG 490

Query: 484 YHFYVVGSGFGNFNPATDPARFNLIDPPVRNTIGTPPGGWVAIRFVADNPGAWLMHCHID 543
           Y+FYVVGSGFGNF+   DP R+NL+DPP   T+G P  GW A+RFVA+NPG WL+HCHI+
Sbjct: 491 YNFYVVGSGFGNFDRRKDPLRYNLVDPPEETTVGVPRNGWTAVRFVANNPGVWLLHCHIE 550

Query: 544 SHLAWGLAMVFLVENGEGELQSVLPPPPDLPPC 575
            H  WG+  VF+V++G  +   ++ PPPDLP C
Sbjct: 551 RHATWGMNTVFIVKDGPTKSSRMVKPPPDLPSC 569

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q56YT03.0e-25973.91Laccase-3 OS=Arabidopsis thaliana OX=3702 GN=LAC3 PE=2 SV=2[more]
Q9LYQ22.8e-25775.13Laccase-13 OS=Arabidopsis thaliana OX=3702 GN=LAC13 PE=2 SV=1[more]
Q9SIY81.0e-22263.33Laccase-5 OS=Arabidopsis thaliana OX=3702 GN=LAC5 PE=2 SV=1[more]
Q9FLB56.1e-22063.91Laccase-12 OS=Arabidopsis thaliana OX=3702 GN=LAC12 PE=2 SV=1[more]
Q941X21.4e-21663.59Laccase-3 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I6510.0e+00100.00Laccase OS=Cucurbita maxima OX=3661 GN=LOC111469556 PE=3 SV=1[more]
A0A6J1HPK10.0e+0098.43Laccase OS=Cucurbita moschata OX=3662 GN=LOC111464877 PE=3 SV=1[more]
A0A6J1K8J59.4e-30989.37Laccase OS=Cucurbita maxima OX=3661 GN=LOC111493234 PE=3 SV=1[more]
A0A6J1G9571.2e-30688.33Laccase OS=Cucurbita moschata OX=3662 GN=LOC111452103 PE=3 SV=1[more]
A0A5D3BDS81.0e-29986.28Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00710 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022970624.10.0e+00100.00laccase-13-like [Cucurbita maxima][more]
XP_023520100.10.0e+0098.95laccase-13-like [Cucurbita pepo subsp. pepo][more]
XP_022964914.10.0e+0098.43laccase-13-like [Cucurbita moschata][more]
KAG7019107.10.0e+0098.69Laccase-3, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038895520.11.3e-30988.37laccase-13-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G30210.12.1e-26073.91laccase 3 [more]
AT5G07130.12.0e-25875.13laccase 13 [more]
AT2G40370.17.1e-22463.33laccase 5 [more]
AT5G05390.14.3e-22163.91laccase 12 [more]
AT5G09360.14.0e-17451.66laccase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 44..161
e-value: 4.5E-36
score: 126.0
IPR008972CupredoxinGENE3D2.60.40.420coord: 381..574
e-value: 1.5E-61
score: 209.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 164..353
e-value: 2.2E-44
score: 153.9
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 357..574
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 31..179
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 164..351
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 40..153
e-value: 2.4E-42
score: 143.7
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 424..557
e-value: 3.6E-41
score: 140.1
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 169..316
e-value: 3.5E-42
score: 144.2
IPR017761LaccaseTIGRFAMTIGR03389TIGR03389coord: 31..574
e-value: 3.2E-251
score: 832.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 322..345
NoneNo IPR availablePANTHERPTHR11709:SF68LACCASE-13coord: 7..574
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 7..574
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 537..548
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 532..552
IPR034289Laccase, third cupredoxin domainCDDcd13897CuRO_3_LCC_plantcoord: 419..557
e-value: 5.84515E-86
score: 261.425
IPR034288Laccase, first cupredoxin domainCDDcd13849CuRO_1_LCC_plantcoord: 36..152
e-value: 2.93171E-70
score: 219.823
IPR034285Laccase, second cupredoxin domainCDDcd13875CuRO_2_LCC_plantcoord: 169..314
e-value: 4.098E-85
score: 259.453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G000340.1CmaCh13G000340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046274 lignin catabolic process
cellular_component GO:0048046 apoplast
molecular_function GO:0005507 copper ion binding
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity
molecular_function GO:0016491 oxidoreductase activity