CmaCh12G008450 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh12G008450 vs. ExPASy Swiss-Prot
Match: Q8L6J1 (DNA-directed RNA polymerase 3B, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPOT3-TOM PE=2 SV=2)

HSP 1 Score: 1350.1 bits (3493), Expect = 0.0e+00
Identity = 684/1000 (68.40%), Postives = 817/1000 (81.70%), Query Frame = 0

Query: 1   MHSTASFFPTHQALIYGGNAWREPSKRCKCQRKTHLSFTSTFNSLSNSHISSVSFPINFD 60
           M STAS+ P+        + WR  +++   +   ++     F  LS S   S S   +F 
Sbjct: 1   MASTASYSPSPT------SQWR--TQKLPKRFNFYVIHNQEFGKLSQSSSLSTS---SFP 60

Query: 61  STAEPLRLQPPADSVQENFTEALDNSMNLQISKSEIHQITPG----EPCQRIFIRDPPWI 120
            T +   +  P +++Q   T  +    NL+     + +I  G    E  +R+FI+DPPW+
Sbjct: 61  KTLKLPVIHMPINNIQSQTTVCVSTDENLE-ELVNLQKIPNGFLNKESNKRVFIQDPPWV 120

Query: 121 ASLFLKGLYKSLQ--KGVKLEFREIE-KRKYNLLRRRQLKAETEAWEKTVEEYKELEREM 180
           +SLF+  L+   +  +GV+ EFREIE +R+Y +LRRRQ+KAETEAWE+ VEEY+ELEREM
Sbjct: 121 SSLFMNSLFVRAKQVQGVRREFREIERRRRYAMLRRRQIKAETEAWEQMVEEYRELEREM 180

Query: 181 CEKKLAPNLPYVKSLFLGWFEPLKQAIEKEQKTQRSKKQKAAFAPHIELLSAEKMAVIVM 240
           CEKKLAPNLPYVK L LGWFEPL+QAIEKEQ  + + K +AAFAPHI+ L A+KMAVIVM
Sbjct: 181 CEKKLAPNLPYVKKLLLGWFEPLRQAIEKEQNAETTVKHRAAFAPHIDSLPADKMAVIVM 240

Query: 241 YKMMGLLMV-AQQDGCVLVVQAAVQIGSAIEQEVRIQSFL-KARSNQRTKVPADAQ-CSN 300
           +K+MGLLM+  +++ CV VVQAAVQIG A+E EVRI +FL K +  Q+    A +Q   +
Sbjct: 241 HKLMGLLMMGGKEERCVQVVQAAVQIGMAVENEVRIHNFLEKTKKLQKHMTGAQSQEDMS 300

Query: 301 QEKELLRKRVNSLIRRRRLKEVQKLLKKEEFKDWGRDTQAKLGSRLIELLIESAFIQPPV 360
           +E  +LRKRV SLI+R R+ EV+KL+K EE + WGRDTQAKLG RL+ELL E+A++Q PV
Sbjct: 301 KETMILRKRVKSLIKRNRVVEVRKLMKSEEPESWGRDTQAKLGCRLLELLTETAYVQRPV 360

Query: 361 TQSADSPPDIRPAFWHKFKPVAKTPGKNIVKNYGVIECDPIVLTGLDRTAKHMLIPYIPM 420
            QSAD+PPDIRPAF H FK   + PGKNIVK YGVIECDP+V+ G+DRT K M+IPY+PM
Sbjct: 361 DQSADTPPDIRPAFRHVFKIATRDPGKNIVKKYGVIECDPLVVAGVDRTVKQMMIPYVPM 420

Query: 421 LIPPKKWKGYDKGGHLFLPSYVMRTHGSSRQQDALKKISGKPMQKVFEALDMLGSTKWRV 480
           L+PPKKW+GYDKGG+LFLPSY+MRTHGS RQQDA++ +  K MQ+V+EALD LGSTKWRV
Sbjct: 421 LVPPKKWRGYDKGGYLFLPSYLMRTHGSRRQQDAVRGVPTKQMQQVYEALDTLGSTKWRV 480

Query: 481 NRRVLSVVESIWEQGGNTAGLVDRKDVPIPEKPLGGDLTEMQEWKWSMRKAKKINQELHS 540
           N+R+L+VVESIW  GGN AGLVDRKDVPIPE     D+ E+++WKW +RK+KKINQELHS
Sbjct: 481 NKRILNVVESIWAGGGNIAGLVDRKDVPIPELSNSDDIMEVKKWKWKVRKSKKINQELHS 540

Query: 541 QRCDIELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGVLEFAEGRPL 600
           QRCD ELKLSVARK+KDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRG+LEFAEGRPL
Sbjct: 541 QRCDTELKLSVARKLKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGILEFAEGRPL 600

Query: 601 GKSGLHWLKIHLANLYAGGVEKLSYDRRLAFVDDHLDSIFDSASNPVKGDRWWLTAEDPF 660
           GKSGL WLKIHLA+LYAGGVEKL YD RLAFV++H+  I DSA+NP+ G+RWWL AEDPF
Sbjct: 601 GKSGLRWLKIHLASLYAGGVEKLCYDARLAFVENHIHDILDSANNPLNGNRWWLNAEDPF 660

Query: 661 QCLAACINLFEALKSSSPHTVISHLPIHQDGSCNGLQHYAALGRDTLEAAAVNLVAGEEF 720
           QCLAACINL EALKSSSPHTVISHLPIHQDGSCNGLQHYAALGRD++EAAAVNLVAGE+ 
Sbjct: 661 QCLAACINLSEALKSSSPHTVISHLPIHQDGSCNGLQHYAALGRDSMEAAAVNLVAGEKP 720

Query: 721 ADVYSEIAARVHDIMIRDGNKDPTANPNALLAKILIDQVDRKLVKQTVMTSVYGVTYVGA 780
           ADVY+EIA RV  I+  D  KDP  +PNALLAK+LIDQVDRKLVKQTVMTSVYGVTYVGA
Sbjct: 721 ADVYTEIALRVDHIIRGDSIKDPAIDPNALLAKLLIDQVDRKLVKQTVMTSVYGVTYVGA 780

Query: 781 REQIKRRLEEKGIISDDRLLFRAACYAAKVTLAALGEIFEAARGIMSWLGDCAKACHSDL 840
           REQIKRRLEEKG+I DDRLLF A+CYAAKVTLAALGE+F+AARG M+WLGDCAK      
Sbjct: 781 REQIKRRLEEKGLIDDDRLLFTASCYAAKVTLAALGELFQAARGTMTWLGDCAK------ 840

Query: 841 FLAGYVIAADNQPVRWTTPLGLPVVQPYYKSERHLIRTSLQVLALRREGNSVDVRKQRTA 900
                VIA +NQPVRWTTPLGLPVVQPY+K++RH+IRTSLQ+LAL+REG++V+VRKQRTA
Sbjct: 841 -----VIALENQPVRWTTPLGLPVVQPYFKTQRHVIRTSLQILALQREGDAVEVRKQRTA 900

Query: 901 FPPNFVHSLDGSHMMLTALACRDAGLRFAGVHDSFWTHACDVDQMNLILREKFVELYNMP 960
           FPPNFVHSLDGSHMM+TA+ACRDAGL+FAGVHDSFWTHACDVDQMN ILREKFVELY++P
Sbjct: 901 FPPNFVHSLDGSHMMMTAVACRDAGLQFAGVHDSFWTHACDVDQMNRILREKFVELYSLP 960

Query: 961 VLETLLEDFETTYPGLTFPPLPERGNFDLREVLKSRYFFN 991
           +LE LLE+F+ +YP LTFPPLP+RG+F+LREVL+S YFFN
Sbjct: 961 ILEDLLENFQKSYPALTFPPLPKRGDFNLREVLESPYFFN 977

BLAST of CmaCh12G008450 vs. ExPASy Swiss-Prot
Match: P69243 (DNA-directed RNA polymerase 3A, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPOT3-SYL PE=3 SV=1)

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 683/1000 (68.30%), Postives = 821/1000 (82.10%), Query Frame = 0

Query: 1   MHSTASFFPTHQALIYGGNAWREPSKRCKCQRKTHLSFTSTFNSLS-NSHISSVSFPINF 60
           M STAS+ P+        + WR  +++   +   ++     F  LS +S + + SFP   
Sbjct: 1   MASTASYSPSPT------SQWR--TQKLPKRFNFYVIHNQEFGKLSQSSSLPTSSFP--- 60

Query: 61  DSTAEPLRLQPPADSVQENFTEALDNSMNLQ--ISKSEI-HQITPGEPCQRIFIRDPPWI 120
            +   P+   P  +++Q   T  +    NL+  ++  +I + +   E  +R+FI+DPPW+
Sbjct: 61  KTLKLPVIQMPINNNIQSQTTVCVSTDENLEELVNLQKIANGVLTKESNKRVFIQDPPWV 120

Query: 121 ASLFLKGLYKSLQ--KGVKLEFREIE-KRKYNLLRRRQLKAETEAWEKTVEEYKELEREM 180
           +SLF+  L+   +  +GV+ EFREIE +R+Y +LRRRQ+KAETEAWE+ VEEY+ELEREM
Sbjct: 121 SSLFMNSLFVRAKQVQGVRREFREIERRRRYAMLRRRQIKAETEAWEQMVEEYRELEREM 180

Query: 181 CEKKLAPNLPYVKSLFLGWFEPLKQAIEKEQKTQRSKKQKAAFAPHIELLSAEKMAVIVM 240
           CEKKLAPNLPYVK L LGWFEPL+QAIEKEQ  + + K +AAFAPHI+ L A+KMAVIVM
Sbjct: 181 CEKKLAPNLPYVKKLLLGWFEPLRQAIEKEQNAETTVKHRAAFAPHIDSLPADKMAVIVM 240

Query: 241 YKMMGLLMV-AQQDGCVLVVQAAVQIGSAIEQEVRIQSFL-KARSNQRTKVPADAQ-CSN 300
           +K+MGLLM+  +++ CV VVQAAVQIG A+E EVRI +FL K +  Q+    A +Q   +
Sbjct: 241 HKLMGLLMMGGKEERCVQVVQAAVQIGMAVENEVRIHNFLEKTKKLQKHMTGAQSQEDMS 300

Query: 301 QEKELLRKRVNSLIRRRRLKEVQKLLKKEEFKDWGRDTQAKLGSRLIELLIESAFIQPPV 360
           +E  +LRKRV SLI+R R+ EV+KL+K EE + WGRDTQAKLG RL+ELL E+A++QPPV
Sbjct: 301 RETMILRKRVKSLIKRNRVVEVRKLMKSEEPESWGRDTQAKLGCRLLELLTETAYVQPPV 360

Query: 361 TQSADSPPDIRPAFWHKFKPVAKTPGKNIVKNYGVIECDPIVLTGLDRTAKHMLIPYIPM 420
            QSAD+PPDIRPAF H F+   + PGK+IVK YGVIECDP+V+ G+DRT K M+IPY+PM
Sbjct: 361 DQSADTPPDIRPAFRHVFRIATRDPGKSIVKKYGVIECDPLVVAGVDRTVKQMMIPYVPM 420

Query: 421 LIPPKKWKGYDKGGHLFLPSYVMRTHGSSRQQDALKKISGKPMQKVFEALDMLGSTKWRV 480
           L+PPKKW+GYDKGG+LFLPSY+MRTHGS RQQDA++ +  K MQ+V+EALD LGSTKWRV
Sbjct: 421 LVPPKKWRGYDKGGYLFLPSYLMRTHGSRRQQDAVRSVPTKQMQQVYEALDTLGSTKWRV 480

Query: 481 NRRVLSVVESIWEQGGNTAGLVDRKDVPIPEKPLGGDLTEMQEWKWSMRKAKKINQELHS 540
           N+R+LSVVESIW  GGN AGLVDRKDVPIPE     D+ E+++WKW +RK+KKINQELHS
Sbjct: 481 NKRILSVVESIWAGGGNIAGLVDRKDVPIPELH-SDDIMEVKKWKWRVRKSKKINQELHS 540

Query: 541 QRCDIELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGVLEFAEGRPL 600
           QRCD ELKLSVARK+KDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRG+LEFAEGRPL
Sbjct: 541 QRCDTELKLSVARKLKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGILEFAEGRPL 600

Query: 601 GKSGLHWLKIHLANLYAGGVEKLSYDRRLAFVDDHLDSIFDSASNPVKGDRWWLTAEDPF 660
           GKSGL WLKIHLA+LYAGG+EKL YD RLAFV++H+D I DSA+NP+ G+RWWL AEDPF
Sbjct: 601 GKSGLRWLKIHLASLYAGGIEKLCYDARLAFVENHIDDILDSANNPLNGNRWWLNAEDPF 660

Query: 661 QCLAACINLFEALKSSSPHTVISHLPIHQDGSCNGLQHYAALGRDTLEAAAVNLVAGEEF 720
           QCLAACINL EALKSSSPHTV SHLPIHQDGSCNGLQHYAALGRD++EAAAVNLVAG++ 
Sbjct: 661 QCLAACINLSEALKSSSPHTVFSHLPIHQDGSCNGLQHYAALGRDSMEAAAVNLVAGDKP 720

Query: 721 ADVYSEIAARVHDIMIRDGNKDPTANPNALLAKILIDQVDRKLVKQTVMTSVYGVTYVGA 780
           ADVY+EIA RV  I+  D  KDP  +PNALLAK+LIDQVDRKLVKQTVMTSVYGVTYVGA
Sbjct: 721 ADVYTEIALRVDHIIRGDSIKDPATDPNALLAKLLIDQVDRKLVKQTVMTSVYGVTYVGA 780

Query: 781 REQIKRRLEEKGIISDDRLLFRAACYAAKVTLAALGEIFEAARGIMSWLGDCAKACHSDL 840
           REQIKRRLEEKG+I DDRLLF A+CYAAKVTLAALGE+F+AARG M+WLGDCAK      
Sbjct: 781 REQIKRRLEEKGLIDDDRLLFTASCYAAKVTLAALGELFQAARGTMTWLGDCAK------ 840

Query: 841 FLAGYVIAADNQPVRWTTPLGLPVVQPYYKSERHLIRTSLQVLALRREGNSVDVRKQRTA 900
                VIA++NQPVRWTTPLGLPVVQPY+K++RH+IRTSLQVLAL+REG++V+VRKQRTA
Sbjct: 841 -----VIASENQPVRWTTPLGLPVVQPYFKTQRHVIRTSLQVLALQREGDTVEVRKQRTA 900

Query: 901 FPPNFVHSLDGSHMMLTALACRDAGLRFAGVHDSFWTHACDVDQMNLILREKFVELYNMP 960
           FPPNFVHSLDGSHMM+TA+ACRDAGL+FAGVHDSFWTHACDVDQMN ILREKFVELY+MP
Sbjct: 901 FPPNFVHSLDGSHMMMTAVACRDAGLQFAGVHDSFWTHACDVDQMNRILREKFVELYSMP 960

Query: 961 VLETLLEDFETTYPGLTFPPLPERGNFDLREVLKSRYFFN 991
           +LE LLE F+ +YP LTFPPLP+RG+FDL EVL+S YFFN
Sbjct: 961 ILEDLLESFQNSYPALTFPPLPKRGDFDLVEVLESPYFFN 977

BLAST of CmaCh12G008450 vs. ExPASy Swiss-Prot
Match: P69242 (DNA-directed RNA polymerase 3, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=RPOT3 PE=2 SV=1)

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 683/1000 (68.30%), Postives = 821/1000 (82.10%), Query Frame = 0

Query: 1   MHSTASFFPTHQALIYGGNAWREPSKRCKCQRKTHLSFTSTFNSLS-NSHISSVSFPINF 60
           M STAS+ P+        + WR  +++   +   ++     F  LS +S + + SFP   
Sbjct: 1   MASTASYSPSPT------SQWR--TQKLPKRFNFYVIHNQEFGKLSQSSSLPTSSFP--- 60

Query: 61  DSTAEPLRLQPPADSVQENFTEALDNSMNLQ--ISKSEI-HQITPGEPCQRIFIRDPPWI 120
            +   P+   P  +++Q   T  +    NL+  ++  +I + +   E  +R+FI+DPPW+
Sbjct: 61  KTLKLPVIQMPINNNIQSQTTVCVSTDENLEELVNLQKIANGVLTKESNKRVFIQDPPWV 120

Query: 121 ASLFLKGLYKSLQ--KGVKLEFREIE-KRKYNLLRRRQLKAETEAWEKTVEEYKELEREM 180
           +SLF+  L+   +  +GV+ EFREIE +R+Y +LRRRQ+KAETEAWE+ VEEY+ELEREM
Sbjct: 121 SSLFMNSLFVRAKQVQGVRREFREIERRRRYAMLRRRQIKAETEAWEQMVEEYRELEREM 180

Query: 181 CEKKLAPNLPYVKSLFLGWFEPLKQAIEKEQKTQRSKKQKAAFAPHIELLSAEKMAVIVM 240
           CEKKLAPNLPYVK L LGWFEPL+QAIEKEQ  + + K +AAFAPHI+ L A+KMAVIVM
Sbjct: 181 CEKKLAPNLPYVKKLLLGWFEPLRQAIEKEQNAETTVKHRAAFAPHIDSLPADKMAVIVM 240

Query: 241 YKMMGLLMV-AQQDGCVLVVQAAVQIGSAIEQEVRIQSFL-KARSNQRTKVPADAQ-CSN 300
           +K+MGLLM+  +++ CV VVQAAVQIG A+E EVRI +FL K +  Q+    A +Q   +
Sbjct: 241 HKLMGLLMMGGKEERCVQVVQAAVQIGMAVENEVRIHNFLEKTKKLQKHMTGAQSQEDMS 300

Query: 301 QEKELLRKRVNSLIRRRRLKEVQKLLKKEEFKDWGRDTQAKLGSRLIELLIESAFIQPPV 360
           +E  +LRKRV SLI+R R+ EV+KL+K EE + WGRDTQAKLG RL+ELL E+A++QPPV
Sbjct: 301 RETMILRKRVKSLIKRNRVVEVRKLMKSEEPESWGRDTQAKLGCRLLELLTETAYVQPPV 360

Query: 361 TQSADSPPDIRPAFWHKFKPVAKTPGKNIVKNYGVIECDPIVLTGLDRTAKHMLIPYIPM 420
            QSAD+PPDIRPAF H F+   + PGK+IVK YGVIECDP+V+ G+DRT K M+IPY+PM
Sbjct: 361 DQSADTPPDIRPAFRHVFRIATRDPGKSIVKKYGVIECDPLVVAGVDRTVKQMMIPYVPM 420

Query: 421 LIPPKKWKGYDKGGHLFLPSYVMRTHGSSRQQDALKKISGKPMQKVFEALDMLGSTKWRV 480
           L+PPKKW+GYDKGG+LFLPSY+MRTHGS RQQDA++ +  K MQ+V+EALD LGSTKWRV
Sbjct: 421 LVPPKKWRGYDKGGYLFLPSYLMRTHGSRRQQDAVRSVPTKQMQQVYEALDTLGSTKWRV 480

Query: 481 NRRVLSVVESIWEQGGNTAGLVDRKDVPIPEKPLGGDLTEMQEWKWSMRKAKKINQELHS 540
           N+R+LSVVESIW  GGN AGLVDRKDVPIPE     D+ E+++WKW +RK+KKINQELHS
Sbjct: 481 NKRILSVVESIWAGGGNIAGLVDRKDVPIPELH-SDDIMEVKKWKWRVRKSKKINQELHS 540

Query: 541 QRCDIELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGVLEFAEGRPL 600
           QRCD ELKLSVARK+KDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRG+LEFAEGRPL
Sbjct: 541 QRCDTELKLSVARKLKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGILEFAEGRPL 600

Query: 601 GKSGLHWLKIHLANLYAGGVEKLSYDRRLAFVDDHLDSIFDSASNPVKGDRWWLTAEDPF 660
           GKSGL WLKIHLA+LYAGG+EKL YD RLAFV++H+D I DSA+NP+ G+RWWL AEDPF
Sbjct: 601 GKSGLRWLKIHLASLYAGGIEKLCYDARLAFVENHIDDILDSANNPLNGNRWWLNAEDPF 660

Query: 661 QCLAACINLFEALKSSSPHTVISHLPIHQDGSCNGLQHYAALGRDTLEAAAVNLVAGEEF 720
           QCLAACINL EALKSSSPHTV SHLPIHQDGSCNGLQHYAALGRD++EAAAVNLVAG++ 
Sbjct: 661 QCLAACINLSEALKSSSPHTVFSHLPIHQDGSCNGLQHYAALGRDSMEAAAVNLVAGDKP 720

Query: 721 ADVYSEIAARVHDIMIRDGNKDPTANPNALLAKILIDQVDRKLVKQTVMTSVYGVTYVGA 780
           ADVY+EIA RV  I+  D  KDP  +PNALLAK+LIDQVDRKLVKQTVMTSVYGVTYVGA
Sbjct: 721 ADVYTEIALRVDHIIRGDSIKDPATDPNALLAKLLIDQVDRKLVKQTVMTSVYGVTYVGA 780

Query: 781 REQIKRRLEEKGIISDDRLLFRAACYAAKVTLAALGEIFEAARGIMSWLGDCAKACHSDL 840
           REQIKRRLEEKG+I DDRLLF A+CYAAKVTLAALGE+F+AARG M+WLGDCAK      
Sbjct: 781 REQIKRRLEEKGLIDDDRLLFTASCYAAKVTLAALGELFQAARGTMTWLGDCAK------ 840

Query: 841 FLAGYVIAADNQPVRWTTPLGLPVVQPYYKSERHLIRTSLQVLALRREGNSVDVRKQRTA 900
                VIA++NQPVRWTTPLGLPVVQPY+K++RH+IRTSLQVLAL+REG++V+VRKQRTA
Sbjct: 841 -----VIASENQPVRWTTPLGLPVVQPYFKTQRHVIRTSLQVLALQREGDTVEVRKQRTA 900

Query: 901 FPPNFVHSLDGSHMMLTALACRDAGLRFAGVHDSFWTHACDVDQMNLILREKFVELYNMP 960
           FPPNFVHSLDGSHMM+TA+ACRDAGL+FAGVHDSFWTHACDVDQMN ILREKFVELY+MP
Sbjct: 901 FPPNFVHSLDGSHMMMTAVACRDAGLQFAGVHDSFWTHACDVDQMNRILREKFVELYSMP 960

Query: 961 VLETLLEDFETTYPGLTFPPLPERGNFDLREVLKSRYFFN 991
           +LE LLE F+ +YP LTFPPLP+RG+FDL EVL+S YFFN
Sbjct: 961 ILEDLLESFQNSYPALTFPPLPKRGDFDLVEVLESPYFFN 977

BLAST of CmaCh12G008450 vs. ExPASy Swiss-Prot
Match: O24600 (DNA-directed RNA polymerase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPOT3 PE=2 SV=1)

HSP 1 Score: 1344.7 bits (3479), Expect = 0.0e+00
Identity = 654/887 (73.73%), Postives = 762/887 (85.91%), Query Frame = 0

Query: 106 QRIFIRDPPWIASLFLKGLYKSLQKGVKLEFREIEKRKYNLLRRRQLKAETEAWEKTVEE 165
           +R+F++DPPWI++LFLKGL K + + +K+E ++I+KRK++ LRRRQ+K ETEAWE+ V+E
Sbjct: 121 RRLFMQDPPWISALFLKGLSKMVDQTLKIERKDIDKRKFDSLRRRQVKEETEAWERMVDE 180

Query: 166 YKELEREMCEKKLAPNLPYVKSLFLGWFEPLKQAIEKEQKTQ--RSKKQKAAFAPHIELL 225
           Y++LE+EMCEK LAPNLPYVK +FLGWF+PLK  IE+EQK Q  +SKK +AA+APHIELL
Sbjct: 181 YRDLEKEMCEKNLAPNLPYVKHMFLGWFQPLKDVIEREQKLQKNKSKKVRAAYAPHIELL 240

Query: 226 SAEKMAVIVMYKMMGLLMVAQQDGCVLVVQAAVQIGSAIEQEVRIQSFLKARSNQRTKVP 285
            A+KMAVIVM+KMMGL+M   +DGC+ VVQAAV IG AIEQEVRI +FLK     R    
Sbjct: 241 PADKMAVIVMHKMMGLVMSGHEDGCIQVVQAAVSIGIAIEQEVRIHNFLK---RTRKNNA 300

Query: 286 ADAQCSNQEKELLRKRVNSLIRRRRLKEVQKLLKKEEFKDWGRDTQAKLGSRLIELLIES 345
            D+Q   +EK+LLRKRVNSLIRR+R+ +  K++K E  K WGR TQAKLGSRL+ELLIE+
Sbjct: 301 GDSQEELKEKQLLRKRVNSLIRRKRIIDALKVVKSEGTKPWGRATQAKLGSRLLELLIEA 360

Query: 346 AFIQPPVTQSADSPPDIRPAFWHKFKPVAKTPGKNIVKNYGVIECDPIVLTGLDRTAKHM 405
           A++QPP+TQS DS P+ RPAF H+FK V K PG  +V+ YGVIECD ++L GLD++AKHM
Sbjct: 361 AYVQPPLTQSGDSIPEFRPAFRHRFKTVTKYPGSKLVRRYGVIECDSLLLAGLDKSAKHM 420

Query: 406 LIPYIPMLIPPKKWKGYDKGGHLFLPSYVMRTHGSSRQQDALKKISGKPMQKVFEALDML 465
           LIPY+PML+PPK+WKGYDKGG+LFLPSY+MRTHGS +QQDALK IS K   +VFEALD L
Sbjct: 421 LIPYVPMLVPPKRWKGYDKGGYLFLPSYIMRTHGSKKQQDALKDISHKTAHRVFEALDTL 480

Query: 466 GSTKWRVNRRVLSVVESIWEQGGNTAGLVDRKDVPIPEKPLGGDLTEMQEWKWSMRKAKK 525
           G+TKWRVNR +L VVE +W  GGN AGLV+R+DVPIPEKP   D  E+Q WKWS RKA K
Sbjct: 481 GNTKWRVNRNILDVVERLWADGGNIAGLVNREDVPIPEKPSSEDPEELQSWKWSARKANK 540

Query: 526 INQELHSQRCDIELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGVLE 585
           IN+E HS RCD+ELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRG LE
Sbjct: 541 INRERHSLRCDVELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGTLE 600

Query: 586 FAEGRPLGKSGLHWLKIHLANLYAGGVEKLSYDRRLAFVDDHLDSIFDSASNPVKGDRWW 645
           FAEGRPLGKSGLHWLKIHLANLYAGGVEKLS+D RLAFV++HLD I DSA NP+ G RWW
Sbjct: 601 FAEGRPLGKSGLHWLKIHLANLYAGGVEKLSHDARLAFVENHLDDIMDSAENPIHGKRWW 660

Query: 646 LTAEDPFQCLAACINLFEALKSSSPHTVISHLPIHQDGSCNGLQHYAALGRDTLEAAAVN 705
           L AEDPFQCLAAC+ L +ALKS SP++VISHLPIHQDGSCNGLQHYAALGRD+ EAAAVN
Sbjct: 661 LKAEDPFQCLAACVILTQALKSPSPYSVISHLPIHQDGSCNGLQHYAALGRDSFEAAAVN 720

Query: 706 LVAGEEFADVYSEIAARVHDIMIRDGNKDPTANPNALLAKILIDQVDRKLVKQTVMTSVY 765
           LVAGE+ ADVYSEI+ RVH+IM +D +KDP +NP A LAKILI QVDRKLVKQTVMTSVY
Sbjct: 721 LVAGEKPADVYSEISRRVHEIMKKDSSKDPESNPTAALAKILITQVDRKLVKQTVMTSVY 780

Query: 766 GVTYVGAREQIKRRLEEKGIISDDRLLFRAACYAAKVTLAALGEIFEAARGIMSWLGDCA 825
           GVTYVGAREQIKRRLEEKG+I+D+R+LF AACY+AKVTLAALGEIFEAAR IMSWLGDCA
Sbjct: 781 GVTYVGAREQIKRRLEEKGVITDERMLFAAACYSAKVTLAALGEIFEAARAIMSWLGDCA 840

Query: 826 KACHSDLFLAGYVIAADNQPVRWTTPLGLPVVQPYYKSERHLIRTSLQVLALRREGNSVD 885
           K           +IA+DN PVRW TPLGLPVVQPY +SERHLIRTSLQVLAL+REGN+VD
Sbjct: 841 K-----------IIASDNHPVRWITPLGLPVVQPYCRSERHLIRTSLQVLALQREGNTVD 900

Query: 886 VRKQRTAFPPNFVHSLDGSHMMLTALACRDAGLRFAGVHDSFWTHACDVDQMNLILREKF 945
           VRKQRTAFPPNFVHSLDG+HMM+TA+ACR+AGL FAGVHDS+WTHACDVD MN ILREKF
Sbjct: 901 VRKQRTAFPPNFVHSLDGTHMMMTAVACREAGLNFAGVHDSYWTHACDVDTMNRILREKF 960

Query: 946 VELYNMPVLETLLEDFETTYPGLTFPPLPERGNFDLREVLKSRYFFN 991
           VELYN P+LE LL+ F+ +YP L FPP+P+RG+FDL+EVLKS+YFFN
Sbjct: 961 VELYNTPILEDLLQSFQESYPNLVFPPVPKRGDFDLKEVLKSQYFFN 993

BLAST of CmaCh12G008450 vs. ExPASy Swiss-Prot
Match: Q8L6J3 (DNA-directed RNA polymerase 2B, chloroplastic/mitochondrial OS=Nicotiana tabacum OX=4097 GN=RPOT2-TOM PE=2 SV=2)

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 559/853 (65.53%), Postives = 687/853 (80.54%), Query Frame = 0

Query: 142  RKYNLLRRRQLKAETEAWEKTVEEYKELEREMCEKKLAPNLPYVKSLFLGWFEPLKQAIE 201
            RKY  L+RRQ+K ETEAWE+  +EYKEL  +MCE+KLAPNLPYVKSLFLGWFEPL+  I 
Sbjct: 181  RKYQTLKRRQVKVETEAWEQAAKEYKELLFDMCEQKLAPNLPYVKSLFLGWFEPLRDKIA 240

Query: 202  KEQKTQRSKKQKAAFAPHIELLSAEKMAVIVMYKMMGLLMVAQQDGCVLVVQAAVQIGSA 261
            +EQ+     K KAA+A ++  L A+ MAVI M+K+MGLLM     G   VVQAA+ IG A
Sbjct: 241  EEQELCSQGKSKAAYAKYLYQLPADMMAVITMHKLMGLLMTGGDHGTARVVQAALVIGDA 300

Query: 262  IEQEVRIQSFL---KARSNQRTKVPADAQCSNQEKELLRKRVNSLIRRRRLKEVQKLLKK 321
            IEQEVRI +FL   K +  ++ K   D +   QE+E LRK+V +L+++++L+ V +++++
Sbjct: 301  IEQEVRIHNFLEKTKKQKAEKDKQKEDGEHVTQEQEKLRKKVTNLMKKQKLRAVGQIVRR 360

Query: 322  -EEFKDWGRDTQAKLGSRLIELLIESAFIQPPVTQSADSPPDIRPAFWHKFKPVAKTPGK 381
             ++ K WG+D +AK+GSRLIELL+++A+IQPP  Q A  PPDIRPAF H  + VAK   K
Sbjct: 361  QDDSKPWGQDAKAKVGSRLIELLLQTAYIQPPANQLAVDPPDIRPAFLHSVRTVAKET-K 420

Query: 382  NIVKNYGVIECDPIVLTGLDRTAKHMLIPYIPMLIPPKKWKGYDKGGHLFLPSYVMRTHG 441
            +  + YG+I+CD +V  GL+RTA+HM+IPY+PML+PP KW GYDKGGHL+LPSYVMRTHG
Sbjct: 421  SASRRYGIIQCDELVFKGLERTARHMVIPYMPMLVPPVKWTGYDKGGHLYLPSYVMRTHG 480

Query: 442  SSRQQDALKKISGKPMQKVFEALDMLGSTKWRVNRRVLSVVESIWEQGGNTAGLVDRKDV 501
            + +Q++A+K+ S   +Q VFEALD LGSTKWR+N+RVLSV++ IW  GG  A LVDR D 
Sbjct: 481  ARQQREAVKRASRNQLQPVFEALDTLGSTKWRINKRVLSVIDRIWAGGGRLADLVDRDDA 540

Query: 502  PIPEKPLGGDLTEMQEWKWSMRKAKKINQELHSQRCDIELKLSVARKMKDEEGFYYPHNL 561
            P+PE+P   D     +W+W ++  KK N+E HSQRCDIELKL+VARKMKDEEGF+YPHN+
Sbjct: 541  PLPEEPDTEDEALRTKWRWKVKSVKKENRERHSQRCDIELKLAVARKMKDEEGFFYPHNV 600

Query: 562  DFRGRAYPMHPHLNHLSSDLCRGVLEFAEGRPLGKSGLHWLKIHLANLYAGGVEKLSYDR 621
            DFRGRAYPMHPHLNHL SD+CRGVL FAEGRPLG+SGL WLKIHLANL+AGGVEKLS + 
Sbjct: 601  DFRGRAYPMHPHLNHLGSDICRGVLVFAEGRPLGESGLRWLKIHLANLFAGGVEKLSLEG 660

Query: 622  RLAFVDDHLDSIFDSASNPVKGDRWWLTAEDPFQCLAACINLFEALKSSSPHTVISHLPI 681
            R+AF ++H+D IFDSA  P++G RWWL AEDPFQCLA CINL EA++SSSP T ISH+P+
Sbjct: 661  RIAFTENHMDDIFDSADKPLEGRRWWLNAEDPFQCLAVCINLSEAVRSSSPETSISHIPV 720

Query: 682  HQDGSCNGLQHYAALGRDTLEAAAVNLVAGEEFADVYSEIAARVHDIMIRDGNKDPTANP 741
            HQDGSCNGLQHYAALGRD L AAAVNLVAGE+ ADVYS IAARV DIM RD  +DP   P
Sbjct: 721  HQDGSCNGLQHYAALGRDELGAAAVNLVAGEKPADVYSGIAARVLDIMKRDAQRDPAEFP 780

Query: 742  NALLAKILIDQVDRKLVKQTVMTSVYGVTYVGAREQIKRRLEEKGIISDDRLLFRAACYA 801
            +A+ A+ L++QVDRKLVKQTVMTSVYGVTY+GAR+QIKRRL+E+G I+DD  LF AACYA
Sbjct: 781  DAVRARALVNQVDRKLVKQTVMTSVYGVTYIGARDQIKRRLKERGAIADDSELFGAACYA 840

Query: 802  AKVTLAALGEIFEAARGIMSWLGDCAKACHSDLFLAGYVIAADNQPVRWTTPLGLPVVQP 861
            AKVTL ALGE+FEAAR IM+WLG+CAK           +IA++N+PVRWTTPLGLPVVQP
Sbjct: 841  AKVTLTALGEMFEAARSIMTWLGECAK-----------IIASENEPVRWTTPLGLPVVQP 900

Query: 862  YYKSERHLIRTSLQVLALRREGNSVDVRKQRTAFPPNFVHSLDGSHMMLTALACRDAGLR 921
            Y K  RHLI+TSLQ+L L++E   V V++QRTAFPPNF+HSLDGSHMM+TA+ACR AGL 
Sbjct: 901  YRKIGRHLIKTSLQILTLQQETEKVMVKRQRTAFPPNFIHSLDGSHMMMTAVACRRAGLN 960

Query: 922  FAGVHDSFWTHACDVDQMNLILREKFVELYNMPVLETLLEDFETTYPGLTFPPLPERGNF 981
            FAGVHDS+WTHACDVD++N ILREKFVELY  P+LE LLE F+T+YP L FPPLPERG+F
Sbjct: 961  FAGVHDSYWTHACDVDKLNRILREKFVELYETPILEKLLESFQTSYPTLLFPPLPERGDF 1020

Query: 982  DLREVLKSRYFFN 991
            DLR+VL+S YFFN
Sbjct: 1021 DLRDVLESPYFFN 1021

BLAST of CmaCh12G008450 vs. TAIR 10
Match: AT2G24120.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 1344.7 bits (3479), Expect = 0.0e+00
Identity = 654/887 (73.73%), Postives = 762/887 (85.91%), Query Frame = 0

Query: 106 QRIFIRDPPWIASLFLKGLYKSLQKGVKLEFREIEKRKYNLLRRRQLKAETEAWEKTVEE 165
           +R+F++DPPWI++LFLKGL K + + +K+E ++I+KRK++ LRRRQ+K ETEAWE+ V+E
Sbjct: 121 RRLFMQDPPWISALFLKGLSKMVDQTLKIERKDIDKRKFDSLRRRQVKEETEAWERMVDE 180

Query: 166 YKELEREMCEKKLAPNLPYVKSLFLGWFEPLKQAIEKEQKTQ--RSKKQKAAFAPHIELL 225
           Y++LE+EMCEK LAPNLPYVK +FLGWF+PLK  IE+EQK Q  +SKK +AA+APHIELL
Sbjct: 181 YRDLEKEMCEKNLAPNLPYVKHMFLGWFQPLKDVIEREQKLQKNKSKKVRAAYAPHIELL 240

Query: 226 SAEKMAVIVMYKMMGLLMVAQQDGCVLVVQAAVQIGSAIEQEVRIQSFLKARSNQRTKVP 285
            A+KMAVIVM+KMMGL+M   +DGC+ VVQAAV IG AIEQEVRI +FLK     R    
Sbjct: 241 PADKMAVIVMHKMMGLVMSGHEDGCIQVVQAAVSIGIAIEQEVRIHNFLK---RTRKNNA 300

Query: 286 ADAQCSNQEKELLRKRVNSLIRRRRLKEVQKLLKKEEFKDWGRDTQAKLGSRLIELLIES 345
            D+Q   +EK+LLRKRVNSLIRR+R+ +  K++K E  K WGR TQAKLGSRL+ELLIE+
Sbjct: 301 GDSQEELKEKQLLRKRVNSLIRRKRIIDALKVVKSEGTKPWGRATQAKLGSRLLELLIEA 360

Query: 346 AFIQPPVTQSADSPPDIRPAFWHKFKPVAKTPGKNIVKNYGVIECDPIVLTGLDRTAKHM 405
           A++QPP+TQS DS P+ RPAF H+FK V K PG  +V+ YGVIECD ++L GLD++AKHM
Sbjct: 361 AYVQPPLTQSGDSIPEFRPAFRHRFKTVTKYPGSKLVRRYGVIECDSLLLAGLDKSAKHM 420

Query: 406 LIPYIPMLIPPKKWKGYDKGGHLFLPSYVMRTHGSSRQQDALKKISGKPMQKVFEALDML 465
           LIPY+PML+PPK+WKGYDKGG+LFLPSY+MRTHGS +QQDALK IS K   +VFEALD L
Sbjct: 421 LIPYVPMLVPPKRWKGYDKGGYLFLPSYIMRTHGSKKQQDALKDISHKTAHRVFEALDTL 480

Query: 466 GSTKWRVNRRVLSVVESIWEQGGNTAGLVDRKDVPIPEKPLGGDLTEMQEWKWSMRKAKK 525
           G+TKWRVNR +L VVE +W  GGN AGLV+R+DVPIPEKP   D  E+Q WKWS RKA K
Sbjct: 481 GNTKWRVNRNILDVVERLWADGGNIAGLVNREDVPIPEKPSSEDPEELQSWKWSARKANK 540

Query: 526 INQELHSQRCDIELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGVLE 585
           IN+E HS RCD+ELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRG LE
Sbjct: 541 INRERHSLRCDVELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGTLE 600

Query: 586 FAEGRPLGKSGLHWLKIHLANLYAGGVEKLSYDRRLAFVDDHLDSIFDSASNPVKGDRWW 645
           FAEGRPLGKSGLHWLKIHLANLYAGGVEKLS+D RLAFV++HLD I DSA NP+ G RWW
Sbjct: 601 FAEGRPLGKSGLHWLKIHLANLYAGGVEKLSHDARLAFVENHLDDIMDSAENPIHGKRWW 660

Query: 646 LTAEDPFQCLAACINLFEALKSSSPHTVISHLPIHQDGSCNGLQHYAALGRDTLEAAAVN 705
           L AEDPFQCLAAC+ L +ALKS SP++VISHLPIHQDGSCNGLQHYAALGRD+ EAAAVN
Sbjct: 661 LKAEDPFQCLAACVILTQALKSPSPYSVISHLPIHQDGSCNGLQHYAALGRDSFEAAAVN 720

Query: 706 LVAGEEFADVYSEIAARVHDIMIRDGNKDPTANPNALLAKILIDQVDRKLVKQTVMTSVY 765
           LVAGE+ ADVYSEI+ RVH+IM +D +KDP +NP A LAKILI QVDRKLVKQTVMTSVY
Sbjct: 721 LVAGEKPADVYSEISRRVHEIMKKDSSKDPESNPTAALAKILITQVDRKLVKQTVMTSVY 780

Query: 766 GVTYVGAREQIKRRLEEKGIISDDRLLFRAACYAAKVTLAALGEIFEAARGIMSWLGDCA 825
           GVTYVGAREQIKRRLEEKG+I+D+R+LF AACY+AKVTLAALGEIFEAAR IMSWLGDCA
Sbjct: 781 GVTYVGAREQIKRRLEEKGVITDERMLFAAACYSAKVTLAALGEIFEAARAIMSWLGDCA 840

Query: 826 KACHSDLFLAGYVIAADNQPVRWTTPLGLPVVQPYYKSERHLIRTSLQVLALRREGNSVD 885
           K           +IA+DN PVRW TPLGLPVVQPY +SERHLIRTSLQVLAL+REGN+VD
Sbjct: 841 K-----------IIASDNHPVRWITPLGLPVVQPYCRSERHLIRTSLQVLALQREGNTVD 900

Query: 886 VRKQRTAFPPNFVHSLDGSHMMLTALACRDAGLRFAGVHDSFWTHACDVDQMNLILREKF 945
           VRKQRTAFPPNFVHSLDG+HMM+TA+ACR+AGL FAGVHDS+WTHACDVD MN ILREKF
Sbjct: 901 VRKQRTAFPPNFVHSLDGTHMMMTAVACREAGLNFAGVHDSYWTHACDVDTMNRILREKF 960

Query: 946 VELYNMPVLETLLEDFETTYPGLTFPPLPERGNFDLREVLKSRYFFN 991
           VELYN P+LE LL+ F+ +YP L FPP+P+RG+FDL+EVLKS+YFFN
Sbjct: 961 VELYNTPILEDLLQSFQESYPNLVFPPVPKRGDFDLKEVLKSQYFFN 993

BLAST of CmaCh12G008450 vs. TAIR 10
Match: AT2G24120.2 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 569/779 (73.04%), Postives = 663/779 (85.11%), Query Frame = 0

Query: 106 QRIFIRDPPWIASLFLKGLYKSLQKGVKLEFREIEKRKYNLLRRRQLKAETEAWEKTVEE 165
           +R+F++DPPWI++LFLKGL K + + +K+E ++I+KRK++ LRRRQ+K ETEAWE+ V+E
Sbjct: 121 RRLFMQDPPWISALFLKGLSKMVDQTLKIERKDIDKRKFDSLRRRQVKEETEAWERMVDE 180

Query: 166 YKELEREMCEKKLAPNLPYVKSLFLGWFEPLKQAIEKEQKTQ--RSKKQKAAFAPHIELL 225
           Y++LE+EMCEK LAPNLPYVK +FLGWF+PLK  IE+EQK Q  +SKK +AA+APHIELL
Sbjct: 181 YRDLEKEMCEKNLAPNLPYVKHMFLGWFQPLKDVIEREQKLQKNKSKKVRAAYAPHIELL 240

Query: 226 SAEKMAVIVMYKMMGLLMVAQQDGCVLVVQAAVQIGSAIEQEVRIQSFLKARSNQRTKVP 285
            A+KMAVIVM+KMMGL+M   +DGC+ VVQAAV IG AIEQEVRI +FLK     R    
Sbjct: 241 PADKMAVIVMHKMMGLVMSGHEDGCIQVVQAAVSIGIAIEQEVRIHNFLK---RTRKNNA 300

Query: 286 ADAQCSNQEKELLRKRVNSLIRRRRLKEVQKLLKKEEFKDWGRDTQAKLGSRLIELLIES 345
            D+Q   +EK+LLRKRVNSLIRR+R+ +  K++K E  K WGR TQAKLGSRL+ELLIE+
Sbjct: 301 GDSQEELKEKQLLRKRVNSLIRRKRIIDALKVVKSEGTKPWGRATQAKLGSRLLELLIEA 360

Query: 346 AFIQPPVTQSADSPPDIRPAFWHKFKPVAKTPGKNIVKNYGVIECDPIVLTGLDRTAKHM 405
           A++QPP+TQS DS P+ RPAF H+FK V K PG  +V+ YGVIECD ++L GLD++AKHM
Sbjct: 361 AYVQPPLTQSGDSIPEFRPAFRHRFKTVTKYPGSKLVRRYGVIECDSLLLAGLDKSAKHM 420

Query: 406 LIPYIPMLIPPKKWKGYDKGGHLFLPSYVMRTHGSSRQQDALKKISGKPMQKVFEALDML 465
           LIPY+PML+PPK+WKGYDKGG+LFLPSY+MRTHGS +QQDALK IS K   +VFEALD L
Sbjct: 421 LIPYVPMLVPPKRWKGYDKGGYLFLPSYIMRTHGSKKQQDALKDISHKTAHRVFEALDTL 480

Query: 466 GSTKWRVNRRVLSVVESIWEQGGNTAGLVDRKDVPIPEKPLGGDLTEMQEWKWSMRKAKK 525
           G+TKWRVNR +L VVE +W  GGN AGLV+R+DVPIPEKP   D  E+Q WKWS RKA K
Sbjct: 481 GNTKWRVNRNILDVVERLWADGGNIAGLVNREDVPIPEKPSSEDPEELQSWKWSARKANK 540

Query: 526 INQELHSQRCDIELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGVLE 585
           IN+E HS RCD+ELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRG LE
Sbjct: 541 INRERHSLRCDVELKLSVARKMKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGTLE 600

Query: 586 FAEGRPLGKSGLHWLKIHLANLYAGGVEKLSYDRRLAFVDDHLDSIFDSASNPVKGDRWW 645
           FAEGRPLGKSGLHWLKIHLANLYAGGVEKLS+D RLAFV++HLD I DSA NP+ G RWW
Sbjct: 601 FAEGRPLGKSGLHWLKIHLANLYAGGVEKLSHDARLAFVENHLDDIMDSAENPIHGKRWW 660

Query: 646 LTAEDPFQCLAACINLFEALKSSSPHTVISHLPIHQDGSCNGLQHYAALGRDTLEAAAVN 705
           L AEDPFQCLAAC+ L +ALKS SP++VISHLPIHQDGSCNGLQHYAALGRD+ EAAAVN
Sbjct: 661 LKAEDPFQCLAACVILTQALKSPSPYSVISHLPIHQDGSCNGLQHYAALGRDSFEAAAVN 720

Query: 706 LVAGEEFADVYSEIAARVHDIMIRDGNKDPTANPNALLAKILIDQVDRKLVKQTVMTSVY 765
           LVAGE+ ADVYSEI+ RVH+IM +D +KDP +NP A LAKILI QVDRKLVKQTVMTSVY
Sbjct: 721 LVAGEKPADVYSEISRRVHEIMKKDSSKDPESNPTAALAKILITQVDRKLVKQTVMTSVY 780

Query: 766 GVTYVGAREQIKRRLEEKGIISDDRLLFRAACYAAKVTLAALGEIFEAARGIMSWLGDCA 825
           GVTYVGAREQIKRRLEEKG+I+D+R+LF AACY+AKVTLAALGEIFEAAR IMSWLGDCA
Sbjct: 781 GVTYVGAREQIKRRLEEKGVITDERMLFAAACYSAKVTLAALGEIFEAARAIMSWLGDCA 840

Query: 826 KACHSDLFLAGYVIAADNQPVRWTTPLGLPVVQPYYKSERHLIRTSLQVLALRREGNSV 883
           K           +IA+DN PVRW TPLGLPVVQPY +SERHLIRTSLQVLAL+REGN+V
Sbjct: 841 K-----------IIASDNHPVRWITPLGLPVVQPYCRSERHLIRTSLQVLALQREGNTV 885

BLAST of CmaCh12G008450 vs. TAIR 10
Match: AT1G68990.1 (male gametophyte defective 3 )

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 530/859 (61.70%), Postives = 662/859 (77.07%), Query Frame = 0

Query: 142 RKYNLLRRRQLKAETEAWEKTVEEYKELEREMCEKKLAPNLPYVKSLFLGWFEPLKQAIE 201
           +K+ +L++RQ+K ETE WE+   E +E+  +MCE+KLAPNLPY+KSLFLGWFEP++ AI+
Sbjct: 130 QKFYMLKQRQVKMETEEWERAARECREILADMCEQKLAPNLPYMKSLFLGWFEPVRNAIQ 189

Query: 202 KEQKTQRSKKQKAAFAPHIELLSAEKMAVIVMYKMMGLLMV-AQQDGCVLVVQAAVQIGS 261
            +  T + KK K  +AP +E L A+KMAVI M+KMMGLLM  A+  G V +V AA QIG 
Sbjct: 190 DDLDTFKIKKGKIPYAPFMEQLPADKMAVITMHKMMGLLMTNAEGVGIVKLVNAATQIGE 249

Query: 262 AIEQEVRIQSFLKARSNQR-TKVPADAQCSN-------QEKELLRKRVNSLIRRRRLKEV 321
           A+EQEVRI SFL+ ++ +  T    + +  N       +E E  RK+V  L+ + +L++V
Sbjct: 250 AVEQEVRINSFLQKKNKKNATDKTINTEAENVSEEIVAKETEKARKQVTVLMEKNKLRQV 309

Query: 322 QKLLKK-EEFKDWGRDTQAKLGSRLIELLIESAFIQPPVTQSADSPPDIRPAFWHKFKPV 381
           + L++K + FK WG++ Q K+G+RLI+LL+E+A+IQPP  Q  D PPDIRPAF   F+ V
Sbjct: 310 KALVRKHDSFKPWGQEAQVKVGARLIQLLMENAYIQPPAEQFDDGPPDIRPAFKQNFRTV 369

Query: 382 AKTPGKNIVKNYGVIECDPIVLTGLDRTAKHMLIPYIPMLIPPKKWKGYDKGGHLFLPSY 441
                K   + YG IECDP+VL GLD++A+HM+IPY+PMLIPP+ W GYD+G H FLPSY
Sbjct: 370 TLENTKT-SRRYGCIECDPLVLKGLDKSARHMVIPYLPMLIPPQNWTGYDQGAHFFLPSY 429

Query: 442 VMRTHGSSRQQDALKKISGKPMQKVFEALDMLGSTKWRVNRRVLSVVESIWEQGGNTAGL 501
           VMRTHG+ +Q+  +K+   + ++ V+EALD LG+TKW++N++VLS+V+ IW  GG   GL
Sbjct: 430 VMRTHGAKQQRTVMKRTPKEQLEPVYEALDTLGNTKWKINKKVLSLVDRIWANGGRIGGL 489

Query: 502 VDRKDVPIPEKPLGGDLTEMQEWKWSMRKAKKINQELHSQRCDIELKLSVARKMKDEEGF 561
           VDR+DVPIPE+P   D  + + W+W  +KA K N E HSQRCDIELKL VARKMKDEEGF
Sbjct: 490 VDREDVPIPEEPEREDQEKFKNWRWESKKAIKQNNERHSQRCDIELKLEVARKMKDEEGF 549

Query: 562 YYPHNLDFRGRAYPMHPHLNHLSSDLCRGVLEFAEGRPLGKSGLHWLKIHLANLYAGGVE 621
           YYPHN+DFRGRAYP+HP+LNHL SDLCRG+LEF EG+PLGKSGL WLKIH+ANLYAGGV+
Sbjct: 550 YYPHNVDFRGRAYPIHPYLNHLGSDLCRGILEFCEGKPLGKSGLRWLKIHIANLYAGGVD 609

Query: 622 KLSYDRRLAFVDDHLDSIFDSASNPVKGDRWWLTAEDPFQCLAACINLFEALKSSSPHTV 681
           KL+Y+ R+AF + HL+ IFDS+  P++G RWWL AEDPFQCLAACINL EAL+S  P   
Sbjct: 610 KLAYEDRIAFTESHLEDIFDSSDRPLEGKRWWLNAEDPFQCLAACINLSEALRSPFPEAA 669

Query: 682 ISHLPIHQDGSCNGLQHYAALGRDTLEAAAVNLVAGEEFADVYSEIAARVHDIMIRDGNK 741
           ISH+PIHQDGSCNGLQHYAALGRD L A AVNLV GE+ ADVY+EIAARV  IM +D  +
Sbjct: 670 ISHIPIHQDGSCNGLQHYAALGRDKLGADAVNLVTGEKPADVYTEIAARVLKIMQQDAEE 729

Query: 742 DPTANPNALLAKILIDQVDRKLVKQTVMTSVYGVTYVGAREQIKRRLEEKGIISDDRLLF 801
           DP   PNA  AK+++DQVDRKLVKQTVMTSVYGVTY GAR+QIK+RL+E+G   DD L F
Sbjct: 730 DPETFPNATYAKLMLDQVDRKLVKQTVMTSVYGVTYSGARDQIKKRLKERGTFEDDSLTF 789

Query: 802 RAACYAAKVTLAALGEIFEAARGIMSWLGDCAKACHSDLFLAGYVIAADNQPVRWTTPLG 861
            A+CYAAK+TL AL E+FEAAR I SW GDCAK           +IA++N  V WTTPLG
Sbjct: 790 HASCYAAKITLKALEEMFEAARAIKSWFGDCAK-----------IIASENNAVCWTTPLG 849

Query: 862 LPVVQPYYKSERHLIRTSLQVLALRREGNSVDVRKQRTAFPPNFVHSLDGSHMMLTALAC 921
           LPVVQPY K  RHL++T+LQVL L RE + V  R+Q TAF PNF+HSLDGSHMM+TA+AC
Sbjct: 850 LPVVQPYRKPGRHLVKTTLQVLTLSRETDKVMARRQMTAFAPNFIHSLDGSHMMMTAVAC 909

Query: 922 RDAGLRFAGVHDSFWTHACDVDQMNLILREKFVELYNMPVLETLLEDFETTYPGLTFPPL 981
             AGL FAGVHDSFWTHACDVD MN ILREKFVELY  P+LE LLE F+ ++P ++FPPL
Sbjct: 910 NRAGLSFAGVHDSFWTHACDVDVMNTILREKFVELYEKPILENLLESFQKSFPDISFPPL 969

Query: 982 PERGNFDLREVLKSRYFFN 991
           PERG+FDLR+VL+S YFFN
Sbjct: 970 PERGDFDLRKVLESTYFFN 976

BLAST of CmaCh12G008450 vs. TAIR 10
Match: AT5G15700.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 536/854 (62.76%), Postives = 665/854 (77.87%), Query Frame = 0

Query: 141  KRKYNLLRRRQLKAETEAWEKTVEEYKELEREMCEKKLAPNLPYVKSLFLGWFEPLKQAI 200
            + K+  L RRQ+K ETE WE+   EY EL  +MCE+KLAPNLPYVKSLFLGWFEPL+ AI
Sbjct: 171  RTKFQNLWRRQVKIETEEWERAAAEYMELLTDMCEQKLAPNLPYVKSLFLGWFEPLRDAI 230

Query: 201  EKEQKTQRSKKQKAAFAPHIELLSAEKMAVIVMYKMMGLLMVAQQDGCVLVVQAAVQIGS 260
             K+Q+  R  K KA +A +++ L A+K++VI M+K+MG LM    +GCV VV AA  +G 
Sbjct: 231  AKDQELYRLGKSKATYAHYLDQLPADKISVITMHKLMGHLMTGGDNGCVKVVHAACTVGD 290

Query: 261  AIEQEVRIQSFL---KARSNQRTKVPADAQCSNQEKELLRKRVNSLIRRRRLKEVQKLLK 320
            AIEQE+RI +FL   K   +       + + S +E++ LRK+VN LI++++L  V+K+L+
Sbjct: 291  AIEQEIRICTFLDKKKKGDDNEESGGVENETSMKEQDKLRKKVNELIKKQKLSAVRKILQ 350

Query: 321  KEEF-KDWGRDTQAKLGSRLIELLIESAFIQPPVTQSADSPPDIRPAFWHKFKPVAKTPG 380
              ++ K W  D +AK+GSRLIELL+ +A+IQ P  Q  +  PD+RPAF H FK VAK   
Sbjct: 351  SHDYTKPWIADVRAKVGSRLIELLVRTAYIQSPADQQDNDLPDVRPAFVHTFK-VAK-GS 410

Query: 381  KNIVKNYGVIECDPIVLTGLDRTAKHMLIPYIPMLIPPKKWKGYDKGGHLFLPSYVMRTH 440
             N  + YGVIECDP+V  GL+++ ++ ++PY+PML+PP KW GYDKG +LFL SY+M+TH
Sbjct: 411  MNSGRKYGVIECDPLVRKGLEKSGRYAVMPYMPMLVPPLKWSGYDKGAYLFLTSYIMKTH 470

Query: 441  GSSRQQDALKKISGKPMQKVFEALDMLGSTKWRVNRRVLSVVESIWEQGGNTAGLVDRKD 500
            G+ +Q++ALK      +Q VFEALD LGSTKWRVN+RVL+VV+ IW  GG  A +VDR D
Sbjct: 471  GAKQQREALKSAPKGQLQPVFEALDTLGSTKWRVNKRVLTVVDRIWSSGGCVADMVDRSD 530

Query: 501  VPIPEKPLGGDLTEMQEWKWSMRKAKKINQELHSQRCDIELKLSVARKMKDEEGFYYPHN 560
            VP+PEKP   D   +++WKW ++ AKK+N E HSQRCD ELKLSVARKMKDEE FYYPHN
Sbjct: 531  VPLPEKPDTEDEGILKKWKWEVKSAKKVNSERHSQRCDTELKLSVARKMKDEEAFYYPHN 590

Query: 561  LDFRGRAYPMHPHLNHLSSDLCRGVLEFAEGRPLGKSGLHWLKIHLANLYAGGVEKLSYD 620
            +DFRGRAYPM PHLNHL SDLCRGVLEFAEGRP+G SGL WLKIHLANLYAGGV+KLS D
Sbjct: 591  MDFRGRAYPMPPHLNHLGSDLCRGVLEFAEGRPMGISGLRWLKIHLANLYAGGVDKLSLD 650

Query: 621  RRLAFVDDHLDSIFDSASNPVKGDRWWLTAEDPFQCLAACINLFEALKSSSPHTVISHLP 680
             RLAF ++HLD IFDSA  P++G RWWL AEDPFQCLA CI+L EAL+S SP TV+SH+P
Sbjct: 651  GRLAFTENHLDDIFDSADRPLEGSRWWLQAEDPFQCLAVCISLTEALRSPSPETVLSHIP 710

Query: 681  IHQDGSCNGLQHYAALGRDTLEAAAVNLVAGEEFADVYSEIAARVHDIMIRDGNKDPTAN 740
            IHQDGSCNGLQHYAALGRDTL A AVNLVAGE+ ADVYS IA RV DIM RD ++DP   
Sbjct: 711  IHQDGSCNGLQHYAALGRDTLGAEAVNLVAGEKPADVYSGIATRVLDIMRRDADRDPEVF 770

Query: 741  PNALLAKILIDQVDRKLVKQTVMTSVYGVTYVGAREQIKRRLEEKGIISDDRLLFRAACY 800
            P AL A+ L++QVDRKLVKQTVMTSVYGVTY+GAR+QIKRRL+E+    D++ +F AACY
Sbjct: 771  PEALRARKLLNQVDRKLVKQTVMTSVYGVTYIGARDQIKRRLKERSDFGDEKEVFGAACY 830

Query: 801  AAKVTLAALGEIFEAARGIMSWLGDCAKACHSDLFLAGYVIAADNQPVRWTTPLGLPVVQ 860
            AAKVTLAA+ E+F+AAR IM W G+CAK           +IA++N+ VRWTTPLGLPVVQ
Sbjct: 831  AAKVTLAAIDEMFQAARAIMRWFGECAK-----------IIASENETVRWTTPLGLPVVQ 890

Query: 861  PYYKSERHLIRTSLQVLALRREGNSVDVRKQRTAFPPNFVHSLDGSHMMLTALACRDAGL 920
            PY++    L++TSLQ L+L+ E + V VR+QRTAFPPNF+HSLDGSHMM+TA+AC+ AG+
Sbjct: 891  PYHQMGTKLVKTSLQTLSLQHETDQVIVRRQRTAFPPNFIHSLDGSHMMMTAVACKRAGV 950

Query: 921  RFAGVHDSFWTHACDVDQMNLILREKFVELYNMPVLETLLEDFETTYPGLTFPPLPERGN 980
             FAGVHDSFWTHACDVD++N+ILREKFVELY+ P+LE LLE FE ++P L FPPLPERG+
Sbjct: 951  CFAGVHDSFWTHACDVDKLNIILREKFVELYSQPILENLLESFEQSFPHLDFPPLPERGD 1010

Query: 981  FDLREVLKSRYFFN 991
             DL+ VL S YFFN
Sbjct: 1011 LDLKVVLDSPYFFN 1011

BLAST of CmaCh12G008450 vs. TAIR 10
Match: AT1G68990.2 (male gametophyte defective 3 )

HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 530/866 (61.20%), Postives = 662/866 (76.44%), Query Frame = 0

Query: 142 RKYNLLRRRQLKAETEAWEKTVEEYKELEREMCEKKLAPNLPYVKSLFLGWFEPLKQAIE 201
           +K+ +L++RQ+K ETE WE+   E +E+  +MCE+KLAPNLPY+KSLFLGWFEP++ AI+
Sbjct: 130 QKFYMLKQRQVKMETEEWERAARECREILADMCEQKLAPNLPYMKSLFLGWFEPVRNAIQ 189

Query: 202 KEQKTQRSKKQKAAFAPHIELLSAEKMAVIVMYKMMGLLMV-AQQDGCVLVVQAAVQIGS 261
            +  T + KK K  +AP +E L A+KMAVI M+KMMGLLM  A+  G V +V AA QIG 
Sbjct: 190 DDLDTFKIKKGKIPYAPFMEQLPADKMAVITMHKMMGLLMTNAEGVGIVKLVNAATQIGE 249

Query: 262 AIEQEVRIQSFLKARSNQR-TKVPADAQCSN-------QEKELLRKRVNSLIRRRRLKEV 321
           A+EQEVRI SFL+ ++ +  T    + +  N       +E E  RK+V  L+ + +L++V
Sbjct: 250 AVEQEVRINSFLQKKNKKNATDKTINTEAENVSEEIVAKETEKARKQVTVLMEKNKLRQV 309

Query: 322 QKLLKK-EEFKDWGRDTQAKLGSRLIELLIESAFIQPPVTQSADSPPDIRPAFWHKFKPV 381
           + L++K + FK WG++ Q K+G+RLI+LL+E+A+IQPP  Q  D PPDIRPAF   F+ V
Sbjct: 310 KALVRKHDSFKPWGQEAQVKVGARLIQLLMENAYIQPPAEQFDDGPPDIRPAFKQNFRTV 369

Query: 382 AKTPGKNIVKNYGVIECDPIVLTGLDRT-------AKHMLIPYIPMLIPPKKWKGYDKGG 441
                K   + YG IECDP+VL GLD++       A+HM+IPY+PMLIPP+ W GYD+G 
Sbjct: 370 TLENTKT-SRRYGCIECDPLVLKGLDKSVSRIVDYARHMVIPYLPMLIPPQNWTGYDQGA 429

Query: 442 HLFLPSYVMRTHGSSRQQDALKKISGKPMQKVFEALDMLGSTKWRVNRRVLSVVESIWEQ 501
           H FLPSYVMRTHG+ +Q+  +K+   + ++ V+EALD LG+TKW++N++VLS+V+ IW  
Sbjct: 430 HFFLPSYVMRTHGAKQQRTVMKRTPKEQLEPVYEALDTLGNTKWKINKKVLSLVDRIWAN 489

Query: 502 GGNTAGLVDRKDVPIPEKPLGGDLTEMQEWKWSMRKAKKINQELHSQRCDIELKLSVARK 561
           GG   GLVDR+DVPIPE+P   D  + + W+W  +KA K N E HSQRCDIELKL VARK
Sbjct: 490 GGRIGGLVDREDVPIPEEPEREDQEKFKNWRWESKKAIKQNNERHSQRCDIELKLEVARK 549

Query: 562 MKDEEGFYYPHNLDFRGRAYPMHPHLNHLSSDLCRGVLEFAEGRPLGKSGLHWLKIHLAN 621
           MKDEEGFYYPHN+DFRGRAYP+HP+LNHL SDLCRG+LEF EG+PLGKSGL WLKIH+AN
Sbjct: 550 MKDEEGFYYPHNVDFRGRAYPIHPYLNHLGSDLCRGILEFCEGKPLGKSGLRWLKIHIAN 609

Query: 622 LYAGGVEKLSYDRRLAFVDDHLDSIFDSASNPVKGDRWWLTAEDPFQCLAACINLFEALK 681
           LYAGGV+KL+Y+ R+AF + HL+ IFDS+  P++G RWWL AEDPFQCLAACINL EAL+
Sbjct: 610 LYAGGVDKLAYEDRIAFTESHLEDIFDSSDRPLEGKRWWLNAEDPFQCLAACINLSEALR 669

Query: 682 SSSPHTVISHLPIHQDGSCNGLQHYAALGRDTLEAAAVNLVAGEEFADVYSEIAARVHDI 741
           S  P   ISH+PIHQDGSCNGLQHYAALGRD L A AVNLV GE+ ADVY+EIAARV  I
Sbjct: 670 SPFPEAAISHIPIHQDGSCNGLQHYAALGRDKLGADAVNLVTGEKPADVYTEIAARVLKI 729

Query: 742 MIRDGNKDPTANPNALLAKILIDQVDRKLVKQTVMTSVYGVTYVGAREQIKRRLEEKGII 801
           M +D  +DP   PNA  AK+++DQVDRKLVKQTVMTSVYGVTY GAR+QIK+RL+E+G  
Sbjct: 730 MQQDAEEDPETFPNATYAKLMLDQVDRKLVKQTVMTSVYGVTYSGARDQIKKRLKERGTF 789

Query: 802 SDDRLLFRAACYAAKVTLAALGEIFEAARGIMSWLGDCAKACHSDLFLAGYVIAADNQPV 861
            DD L F A+CYAAK+TL AL E+FEAAR I SW GDCAK           +IA++N  V
Sbjct: 790 EDDSLTFHASCYAAKITLKALEEMFEAARAIKSWFGDCAK-----------IIASENNAV 849

Query: 862 RWTTPLGLPVVQPYYKSERHLIRTSLQVLALRREGNSVDVRKQRTAFPPNFVHSLDGSHM 921
            WTTPLGLPVVQPY K  RHL++T+LQVL L RE + V  R+Q TAF PNF+HSLDGSHM
Sbjct: 850 CWTTPLGLPVVQPYRKPGRHLVKTTLQVLTLSRETDKVMARRQMTAFAPNFIHSLDGSHM 909

Query: 922 MLTALACRDAGLRFAGVHDSFWTHACDVDQMNLILREKFVELYNMPVLETLLEDFETTYP 981
           M+TA+AC  AGL FAGVHDSFWTHACDVD MN ILREKFVELY  P+LE LLE F+ ++P
Sbjct: 910 MMTAVACNRAGLSFAGVHDSFWTHACDVDVMNTILREKFVELYEKPILENLLESFQKSFP 969

Query: 982 GLTFPPLPERGNFDLREVLKSRYFFN 991
            ++FPPLPERG+FDLR+VL+S YFFN
Sbjct: 970 DISFPPLPERGDFDLRKVLESTYFFN 983