CmaCh12G004710 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G004710
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionLeucine carboxyl methyltransferase
LocationCma_Chr12: 2453683 .. 2455669 (+)
RNA-Seq ExpressionCmaCh12G004710
SyntenyCmaCh12G004710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACCCTCCCTTTTTCTTTAATTGATGAGTTTTTTTCTGAAGCATTGGTTGTGTGTGGTTCTCTTTCCTTATCCCATTCCTTACAATCCTTCTATTTTTTTTTCTCGCACCCTTTTTTTTTTCCTACAAAAACAGAATGCATTATCTGCTTTCCTAGTTCATATCAATTCTAACAGTCAGTTTTTAATATAGAATCATGACTGAAAAAGAAAATGGCTTGGCTGACGACCCAGGGTGGTTTGAGCTAAAGTTTCCGGATCTGTTGTCGGCCGATAGTGTACGTGAAGTCCATGCTAGAATTGTGGCAGAATGGGATTACCTCCAACGTTCGGCGTGTCAAATGGCAGCAGGAAGAGCGCTTTGGAAGCATGTGATTCACGATCCACTAGCAGATTTACTTGCTGGAGAGGCCTGCCTGAGAAACCTGCATGAGAAGATAAAGAAAGATCGAAGCAACAATGCAAGAGAGATGTCTGGAGTGATTCTTGCTGTTCGTACCCTCTGGTTTGATTCAAAGATTGAAGGTGCTCTGACATCCTTCAATGGCAGAGATTCACAAGTCGTTCTCCTTGGTGCAGGTTGGTATTTCAACTCCCAGACACTTGTGCATTTAGTTAACATTCAAGGATAAGAAGATAGAAAAATTGCACAAATAATAACCTCTTTTTTCTTATCTAGCATAACGAGTTCCAATCAGGGTAGTTACTAGGCATGTTAGAACTGCAAAATAAGAACAGCAGTGAAATTAGATCTTACACATTGATATCTAAGATAAAGAAATGTACACACTCCCCTTTTTCCTTTTTCTGTACGTGGGGTAGGAGTGGGAGGAGGGGGATAAAACACTAGGACCGGATCCATGCCTATGAGGCCATCGAAACTGTCTGCTTAATCAAAGAGAATTACTTGCTTGCATCTGAAACATTTGAGATTAAGGGAGAAGACGTATATTAAACTTCAATTTAAGTTTGGTTTGTCACTTAAACAAGTTCAAATAATAGGGACCTCTATTATAATGAAGGAATGGATACGAGGGCTTACAGATTAAGTTGCTTGAAGGAAAGTGATGTTTTTGAAGTTGATTTCCCTGAATTGCTGCAAGCGAAAGCCAATCTTATAAAGATAGCAATAGAATCTCCCTACGAGCACCACCATCATCAACAAATAGCCAAATCGTTAAACAGAGTAGCAGCTGATATCAGAAGTAGTGACTGGCTTGAAAAGCTTCAATCAGCTGGTTTTGCACCAGAGAAGAATACAGTGTGGGTTCTAGAAGGGTTACTCTACTATTTTACCCACTCCCAGGCGATGCAAGTATTGGAGACAATAGCTGACAAATGCAACATAACCCACACAGTCCTCTTAGCAGATTTTATGAACAAACCCTCAACCACCCTTTCAAATTCTGTTTTCCATTTCTACTGTGATTGGCCTGATCATCTACTCCCATCCCTTGGATTTTCCAGCACTAAACTATCACAGATTGGAGACCCAGATGCTCACTTTGGGCTTATGCACGATCCATTAAATCTCTTCAATAAAATCCGCAGTTTACCAAGGTCATTGCAGACTCACCCTGATGATGGAAAACCTTGCTGCCGCTTGTATTTGGTGCAGGCTTCTGGTTCGCCTCTACTACCACCAATCGAATAAAGACAATCTGCAAGTAACATGAACACCTCTGAGTGAGTAGGTTGGATGGATGTGAGATACCACATCTACCTGCTACATGCTTCATTAAAACTACTCCATGGAGAGAATGTCATATTATATTACTCAAGGTCTCTACACCATACCATATCAGAGGTGTAGGCATACACATGTATTCGAATATATATCCGTAAATTATGCATTTAGAATACAGACAACTATTGGACCTAAATTGTGTTTCGTGAATGAGGTATATTATTCCAAGCATGCCCGAATAATGTTCAATCTTCTGCATGTGAAAAGAAATCGCCTATACAAACATCTTTCCTGAAAAGGGG

mRNA sequence

CACCCTCCCTTTTTCTTTAATTGATGAGTTTTTTTCTGAAGCATTGGTTGTGTGTGGTTCTCTTTCCTTATCCCATTCCTTACAATCCTTCTATTTTTTTTTCTCGCACCCTTTTTTTTTTCCTACAAAAACAGAATGCATTATCTGCTTTCCTAGTTCATATCAATTCTAACAGTCAGTTTTTAATATAGAATCATGACTGAAAAAGAAAATGGCTTGGCTGACGACCCAGGGTGGTTTGAGCTAAAGTTTCCGGATCTGTTGTCGGCCGATAGTGTACGTGAAGTCCATGCTAGAATTGTGGCAGAATGGGATTACCTCCAACGTTCGGCGTGTCAAATGGCAGCAGGAAGAGCGCTTTGGAAGCATGTGATTCACGATCCACTAGCAGATTTACTTGCTGGAGAGGCCTGCCTGAGAAACCTGCATGAGAAGATAAAGAAAGATCGAAGCAACAATGCAAGAGAGATGTCTGGAGTGATTCTTGCTGTTCGTACCCTCTGGTTTGATTCAAAGATTGAAGGTGCTCTGACATCCTTCAATGGCAGAGATTCACAAGTCGTTCTCCTTGGTGCAGGAATGGATACGAGGGCTTACAGATTAAGTTGCTTGAAGGAAAGTGATGTTTTTGAAGTTGATTTCCCTGAATTGCTGCAAGCGAAAGCCAATCTTATAAAGATAGCAATAGAATCTCCCTACGAGCACCACCATCATCAACAAATAGCCAAATCGTTAAACAGAGTAGCAGCTGATATCAGAAGTAGTGACTGGCTTGAAAAGCTTCAATCAGCTGGTTTTGCACCAGAGAAGAATACAGTGTGGGTTCTAGAAGGGTTACTCTACTATTTTACCCACTCCCAGGCGATGCAAGTATTGGAGACAATAGCTGACAAATGCAACATAACCCACACAGTCCTCTTAGCAGATTTTATGAACAAACCCTCAACCACCCTTTCAAATTCTGTTTTCCATTTCTACTGTGATTGGCCTGATCATCTACTCCCATCCCTTGGATTTTCCAGCACTAAACTATCACAGATTGGAGACCCAGATGCTCACTTTGGGCTTATGCACGATCCATTAAATCTCTTCAATAAAATCCGCAGTTTACCAAGGTCATTGCAGACTCACCCTGATGATGGAAAACCTTGCTGCCGCTTGTATTTGGTGCAGGCTTCTGGTTCGCCTCTACTACCACCAATCGAATAAAGACAATCTGCAAGTAACATGAACACCTCTGAGTGAGTAGGTTGGATGGATGTGAGATACCACATCTACCTGCTACATGCTTCATTAAAACTACTCCATGGAGAGAATGTCATATTATATTACTCAAGGTCTCTACACCATACCATATCAGAGGTGTAGGCATACACATGTATTCGAATATATATCCGTAAATTATGCATTTAGAATACAGACAACTATTGGACCTAAATTGTGTTTCGTGAATGAGGTATATTATTCCAAGCATGCCCGAATAATGTTCAATCTTCTGCATGTGAAAAGAAATCGCCTATACAAACATCTTTCCTGAAAAGGGG

Coding sequence (CDS)

ATGACTGAAAAAGAAAATGGCTTGGCTGACGACCCAGGGTGGTTTGAGCTAAAGTTTCCGGATCTGTTGTCGGCCGATAGTGTACGTGAAGTCCATGCTAGAATTGTGGCAGAATGGGATTACCTCCAACGTTCGGCGTGTCAAATGGCAGCAGGAAGAGCGCTTTGGAAGCATGTGATTCACGATCCACTAGCAGATTTACTTGCTGGAGAGGCCTGCCTGAGAAACCTGCATGAGAAGATAAAGAAAGATCGAAGCAACAATGCAAGAGAGATGTCTGGAGTGATTCTTGCTGTTCGTACCCTCTGGTTTGATTCAAAGATTGAAGGTGCTCTGACATCCTTCAATGGCAGAGATTCACAAGTCGTTCTCCTTGGTGCAGGAATGGATACGAGGGCTTACAGATTAAGTTGCTTGAAGGAAAGTGATGTTTTTGAAGTTGATTTCCCTGAATTGCTGCAAGCGAAAGCCAATCTTATAAAGATAGCAATAGAATCTCCCTACGAGCACCACCATCATCAACAAATAGCCAAATCGTTAAACAGAGTAGCAGCTGATATCAGAAGTAGTGACTGGCTTGAAAAGCTTCAATCAGCTGGTTTTGCACCAGAGAAGAATACAGTGTGGGTTCTAGAAGGGTTACTCTACTATTTTACCCACTCCCAGGCGATGCAAGTATTGGAGACAATAGCTGACAAATGCAACATAACCCACACAGTCCTCTTAGCAGATTTTATGAACAAACCCTCAACCACCCTTTCAAATTCTGTTTTCCATTTCTACTGTGATTGGCCTGATCATCTACTCCCATCCCTTGGATTTTCCAGCACTAAACTATCACAGATTGGAGACCCAGATGCTCACTTTGGGCTTATGCACGATCCATTAAATCTCTTCAATAAAATCCGCAGTTTACCAAGGTCATTGCAGACTCACCCTGATGATGGAAAACCTTGCTGCCGCTTGTATTTGGTGCAGGCTTCTGGTTCGCCTCTACTACCACCAATCGAATAA

Protein sequence

MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVIHDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSLNRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTVLLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFNKIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE
Homology
BLAST of CmaCh12G004710 vs. ExPASy Swiss-Prot
Match: A8LHA4 (Putative S-adenosyl-L-methionine-dependent methyltransferase Franean1_4929 OS=Frankia sp. (strain EAN1pec) OX=298653 GN=Franean1_4929 PE=3 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 3.1e-14
Identity = 47/140 (33.57%), Postives = 75/140 (53.57%), Query Frame = 0

Query: 91  EMSGVILAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYRLSCLKESDVFEVDFP 150
           EM    LAVRT +FD ++   L +      QVVLL AG+D+RA+RL     + +FEVD P
Sbjct: 82  EMMNAYLAVRTRFFDDEL---LAAAEAGVRQVVLLAAGLDSRAFRLPWPAGTRLFEVDRP 141

Query: 151 ELLQAKANLIKIAIESPYEHHHHQQIAKSLNRVAADIRSSDWLEKLQSAGFAPEKNTVWV 210
           ++L  K  ++      P    H          V+AD+ + DW +++  AGF P + T W+
Sbjct: 142 DILAFKEQVLAAGETGPRCERH---------AVSADL-TEDWADEILDAGFRPAEPTAWL 201

Query: 211 LEGLLYYFTHSQAMQVLETI 231
            EG++ Y +  +A ++L  +
Sbjct: 202 AEGIIVYLSAEEAERLLTDV 208

BLAST of CmaCh12G004710 vs. ExPASy Swiss-Prot
Match: B1MGB7 (Putative S-adenosyl-L-methionine-dependent methyltransferase MAB_3787 OS=Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543) OX=561007 GN=MAB_3787 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.0e-14
Identity = 65/191 (34.03%), Postives = 96/191 (50.26%), Query Frame = 0

Query: 45  SACQMAAGRALWKHV----IHDPLADLLAGEACLRNLHEKIKKDRSN--NAREMSGVI-- 104
           +A  +AA RAL  HV    I+DP A+ L     +    E   K  S   +  +M+G++  
Sbjct: 18  TATMVAAQRALASHVPNPLINDPYAEPLVRAVGI----EYFTKLASGAFDPEQMAGLVQL 77

Query: 105 ----------LAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYRLSCLKESDVFE 164
                     +A RT ++D+  E + T+   R  Q V+L +G+DTRAYRL+    + V+E
Sbjct: 78  GITADGMANGMASRTWYYDTAFESS-TAAGVR--QAVILASGLDTRAYRLTWPAGTTVYE 137

Query: 165 VDFPELLQAKANLIKIAIESPYEHHHHQQIAKSLNRVAADIRSSDWLEKLQSAGFAPEKN 218
           +D PE++  K + +     SP         +     VA D+R  DW   LQ+AGF PE+ 
Sbjct: 138 LDQPEVITFKTDTLAELGASP---------SAERRTVAIDLR-EDWPAALQAAGFDPEQP 191

BLAST of CmaCh12G004710 vs. ExPASy Swiss-Prot
Match: B1MEP9 (Putative S-adenosyl-L-methionine-dependent methyltransferase MAB_0213c OS=Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543) OX=561007 GN=MAB_0213c PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.9e-14
Identity = 61/195 (31.28%), Postives = 97/195 (49.74%), Query Frame = 0

Query: 45  SACQMAAGRALWKHVIH----DPLADLL--AGEACLRNLHEKIKKDRSNNAREMS---GV 104
           +A  +AAGRAL +H  +    DP A+L   A      +L +  + D + ++ +       
Sbjct: 17  TALAVAAGRALARHPANGAPIDPYAELFCRAAGGQWSDLVQGKQSDHALSSEDFGRSFAN 76

Query: 105 ILAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQA 164
             A RT +FD+      T+ +    QVVLL +G+D RAYRL    E++V+E+D P + Q 
Sbjct: 77  FQAARTAFFDNFF---TTTSDTGVRQVVLLASGLDCRAYRLQWPAETEVYELDQPLVQQF 136

Query: 165 KANLIKIAIESPYEHHHHQQIAKSLNRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLL 224
           K   +     +P    H          ++ D+R  DW   LQ +GF P + + W++EGLL
Sbjct: 137 KQETLDAHGAAPSAVRH---------PISVDLR-QDWSTILQESGFDPSRPSAWLVEGLL 196

Query: 225 YYFTHSQAMQVLETI 231
           ++   S    +LETI
Sbjct: 197 FFLKSSAQDLLLETI 198

BLAST of CmaCh12G004710 vs. ExPASy Swiss-Prot
Match: B2HD96 (Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_2791 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=MMAR_2791 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.9e-14
Identity = 54/175 (30.86%), Postives = 80/175 (45.71%), Query Frame = 0

Query: 96  ILAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQA 155
           +  V+T +FD     A  +      Q V++ AG+D+RAYRL     + VFE+D P++L+ 
Sbjct: 80  LYGVQTRFFDDFFRSAAAA---GTKQAVIVAAGLDSRAYRLEWPSGTTVFEIDLPQVLEF 139

Query: 156 KANLIKIAIESPYEHHHHQQIAKSLNRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLL 215
           KA +++          H  +     N VAAD+R +DW   L +AGF P + + W +EGLL
Sbjct: 140 KARVLQ---------RHGVEPKARRNEVAADLR-TDWPATLHAAGFEPGQPSAWSVEGLL 199

Query: 216 YYFTHSQAMQVLETIADKC-------------NITHTVLLADFMNKPSTTLSNSV 258
            Y T      +   I D C              + H  L+A     P   LS  V
Sbjct: 200 PYLTADAQDALFARIDDLCAPGSRIATGALGSRLDHDQLVALEQTHPGVNLSGDV 241

BLAST of CmaCh12G004710 vs. ExPASy Swiss-Prot
Match: B2HLS4 (Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_0356 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=MMAR_0356 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 9.9e-13
Identity = 49/135 (36.30%), Postives = 69/135 (51.11%), Query Frame = 0

Query: 98  AVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKA 157
           AVRT +FD     A         Q V+L AG+D RAYRL+   E+ VFE+D P++L+ KA
Sbjct: 90  AVRTHFFDEYFAAAA---GAGIEQAVILAAGLDCRAYRLNWPPEAVVFEIDQPKVLEYKA 149

Query: 158 NLIKIAIESPYEHHHHQQIAKSLNRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYY 217
            +++          H    A + + VA D+R  DW   L  AGF  ++ T W+ EGLL Y
Sbjct: 150 QILE---------SHGVTAAATRHGVAVDLR-EDWPAALLRAGFDRDRPTAWLAEGLLPY 209

Query: 218 FTHSQAMQVLETIAD 233
                  ++ E I D
Sbjct: 210 LPGDAQDRLFEMITD 211

BLAST of CmaCh12G004710 vs. ExPASy TrEMBL
Match: A0A6J1KNW1 (uncharacterized protein LOC111496264 OS=Cucurbita maxima OX=3661 GN=LOC111496264 PE=3 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 2.3e-198
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60
           MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120
           HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180
           QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180

Query: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240
           NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 338
           KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 337

BLAST of CmaCh12G004710 vs. ExPASy TrEMBL
Match: A0A6J1GI61 (uncharacterized protein LOC111454414 OS=Cucurbita moschata OX=3662 GN=LOC111454414 PE=3 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 2.7e-194
Identity = 330/337 (97.92%), Postives = 332/337 (98.52%), Query Frame = 0

Query: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60
           MTEKENGL DDPGWFELK PD+L ADSVREVHARI AEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLPDDPGWFELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120
           HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180
           QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180

Query: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240
           NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVFHFYCDWP+HLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPEHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 338
           KIRSLPRSLQTHPDDGKPCCRLYLVQASG PLLPPIE
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGLPLLPPIE 337

BLAST of CmaCh12G004710 vs. ExPASy TrEMBL
Match: A0A6J1CRT3 (uncharacterized protein LOC111013987 OS=Momordica charantia OX=3673 GN=LOC111013987 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.1e-174
Identity = 299/338 (88.46%), Postives = 316/338 (93.49%), Query Frame = 0

Query: 1   MTEKENG--LADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKH 60
           MT+KENG  L +DPGW ELK PD+L A+SVRE HARI AEWDYLQRSACQMAAGRALW H
Sbjct: 1   MTDKENGLRLPEDPGWSELKLPDMLLANSVREAHARIEAEWDYLQRSACQMAAGRALWSH 60

Query: 61  VIHDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGR 120
           VIHDPLA LLAGEACLRNLHEKIKKDRSNNARE+SGVILAVRTLWFDSKIEGAL+SFNGR
Sbjct: 61  VIHDPLAGLLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALSSFNGR 120

Query: 121 DSQVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAK 180
           DSQVVLLGAGMDTRAYRLSCLKESDVFEVDFPE+LQ K  L+K AIESPY  HHHQQ+AK
Sbjct: 121 DSQVVLLGAGMDTRAYRLSCLKESDVFEVDFPEVLQVKTALLKTAIESPY-GHHHQQMAK 180

Query: 181 SLNRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITH 240
           SLNR+AADIR +DWLEKLQSAGFA EKNTVWVLEGLLYY THSQAMQVLETIADKC IT 
Sbjct: 181 SLNRIAADIRGNDWLEKLQSAGFASEKNTVWVLEGLLYYLTHSQAMQVLETIADKCTITP 240

Query: 241 TVLLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNL 300
           TVLLADFMNKPSTTLSNS+FHFYCDWPDHLLPSLGFS+TKLSQIGDPDA+FGL+HDPLNL
Sbjct: 241 TVLLADFMNKPSTTLSNSIFHFYCDWPDHLLPSLGFSNTKLSQIGDPDANFGLVHDPLNL 300

Query: 301 FNKIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPI 337
           FNKIRSLPRS+QTHPDDGKPCCRLYLVQASGSP LPP+
Sbjct: 301 FNKIRSLPRSMQTHPDDGKPCCRLYLVQASGSPHLPPL 337

BLAST of CmaCh12G004710 vs. ExPASy TrEMBL
Match: A0A6J1KE32 (uncharacterized protein LOC111492397 OS=Cucurbita maxima OX=3661 GN=LOC111492397 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.0e-173
Identity = 299/335 (89.25%), Postives = 311/335 (92.84%), Query Frame = 0

Query: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60
           MT+KENG  D+PGW ELK PDLL  DSVRE+HARI AEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTDKENGWPDNPGWSELKLPDLLLVDSVREIHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120
           HDPLA+LLAGEACLRNLHEKIKKDRSNNARE+SGVILAVRTLWFDSKIEGALTS NGRDS
Sbjct: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALTSINGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180
           QVVLLGAGMDTRAYRLSCLKESDVFEVDF ELLQAKA L+  AIE P   HHHQQ AKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFSELLQAKATLLNAAIEPP-NGHHHQQKAKSL 180

Query: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240
           NRVAADIRS+DWLEKL++AGFA EKNTVWVLEGLLYY THSQA+QVLETIAD+C ITHTV
Sbjct: 181 NRVAADIRSNDWLEKLKTAGFASEKNTVWVLEGLLYYLTHSQALQVLETIADQCTITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFS+T LSQIGD DAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSTTTLSQIGDMDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPP 336
           KIRSLPRSLQTHPDDGKPCCRLYLVQASGSP L P
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLQP 334

BLAST of CmaCh12G004710 vs. ExPASy TrEMBL
Match: A0A6J1HBI5 (uncharacterized protein LOC111462501 OS=Cucurbita moschata OX=3662 GN=LOC111462501 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.2e-172
Identity = 297/335 (88.66%), Postives = 312/335 (93.13%), Query Frame = 0

Query: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60
           MT+KENG  D+PGW ELK PDLL  DSVRE+HARI AEWDYLQRSACQMAAGRALWKH+I
Sbjct: 1   MTDKENGWPDNPGWSELKLPDLLLVDSVREIHARIEAEWDYLQRSACQMAAGRALWKHMI 60

Query: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120
           HDPLA+LLAGEACLRNL+EKIKKDRSNNARE+SGVILAVRTLWFDSKIEGALTS +GRDS
Sbjct: 61  HDPLAELLAGEACLRNLYEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALTSISGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180
           QVVLLGAGMDTRAYRLSCLKESDVFEVDF ELLQAKA L+K AIE P   HHHQQ AKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFSELLQAKATLLKAAIEPP-NGHHHQQKAKSL 180

Query: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240
           NRVAADIRS+DWLEKL++AGFA EKNTVWVLEGLLYY THSQA+QVLETIAD+C ITHTV
Sbjct: 181 NRVAADIRSNDWLEKLKTAGFASEKNTVWVLEGLLYYLTHSQALQVLETIADQCTITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFS+T LSQIGD DAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSNTTLSQIGDMDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPP 336
           KIRSLPRSLQTHPDDGKPCCRLYLVQASGSP L P
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLQP 334

BLAST of CmaCh12G004710 vs. NCBI nr
Match: XP_023002415.1 (uncharacterized protein LOC111496264 [Cucurbita maxima])

HSP 1 Score: 701.0 bits (1808), Expect = 4.8e-198
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60
           MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120
           HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180
           QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180

Query: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240
           NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 338
           KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 337

BLAST of CmaCh12G004710 vs. NCBI nr
Match: XP_023537171.1 (uncharacterized protein LOC111798329 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 690.3 bits (1780), Expect = 8.5e-195
Identity = 331/337 (98.22%), Postives = 333/337 (98.81%), Query Frame = 0

Query: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60
           MTEKENGL DDPGWFELK PD+L ADSVREVHARI AEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLPDDPGWFELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120
           HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180
           QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180

Query: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240
           NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFS+TKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSTTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 338
           KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 337

BLAST of CmaCh12G004710 vs. NCBI nr
Match: KAG7020511.1 (hypothetical protein SDJN02_17196 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 688.0 bits (1774), Expect = 4.2e-194
Identity = 330/337 (97.92%), Postives = 333/337 (98.81%), Query Frame = 0

Query: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60
           MTEKENGL DDPGWFELK PD+L ADSVREVHARI AEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLPDDPGWFELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120
           HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180
           QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESP+EHHHHQQIAKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPHEHHHHQQIAKSL 180

Query: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240
           NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVFHFYCDWP+HLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPEHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 338
           KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 337

BLAST of CmaCh12G004710 vs. NCBI nr
Match: XP_022951671.1 (uncharacterized protein LOC111454414 [Cucurbita moschata])

HSP 1 Score: 687.6 bits (1773), Expect = 5.5e-194
Identity = 330/337 (97.92%), Postives = 332/337 (98.52%), Query Frame = 0

Query: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60
           MTEKENGL DDPGWFELK PD+L ADSVREVHARI AEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLPDDPGWFELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120
           HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180
           QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180

Query: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240
           NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVFHFYCDWP+HLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPEHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPIE 338
           KIRSLPRSLQTHPDDGKPCCRLYLVQASG PLLPPIE
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGLPLLPPIE 337

BLAST of CmaCh12G004710 vs. NCBI nr
Match: KAG6585597.1 (hypothetical protein SDJN03_18330, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 644.8 bits (1662), Expect = 4.1e-181
Identity = 311/316 (98.42%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 22  LLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVIHDPLADLLAGEACLRNLHEKI 81
           +L ADSVREVHARI AEWDYLQRSACQMAAGRALWKHVIHDPLADLLAGEACLRNLHEKI
Sbjct: 1   MLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVIHDPLADLLAGEACLRNLHEKI 60

Query: 82  KKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYRLSCLKE 141
           KKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYRLSCLKE
Sbjct: 61  KKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYRLSCLKE 120

Query: 142 SDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSLNRVAADIRSSDWLEKLQSAGF 201
           SDVFEVDFPELLQAKANLIKIAIESP+EHHHHQQIAKSLNRVAADIRSSDWLEKLQSAGF
Sbjct: 121 SDVFEVDFPELLQAKANLIKIAIESPHEHHHHQQIAKSLNRVAADIRSSDWLEKLQSAGF 180

Query: 202 APEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTVLLADFMNKPSTTLSNSVFHFY 261
           APEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTVLLADFMNKPSTTLSNSVFHFY
Sbjct: 181 APEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTVLLADFMNKPSTTLSNSVFHFY 240

Query: 262 CDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFNKIRSLPRSLQTHPDDGKPCCR 321
           CDWP+HLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFNKIRSLPRSLQTHPDDGKPCCR
Sbjct: 241 CDWPEHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFNKIRSLPRSLQTHPDDGKPCCR 300

Query: 322 LYLVQASGSPLLPPIE 338
           LYLVQASGSPLLPPIE
Sbjct: 301 LYLVQASGSPLLPPIE 316

BLAST of CmaCh12G004710 vs. TAIR 10
Match: AT5G42760.1 (Leucine carboxyl methyltransferase )

HSP 1 Score: 461.1 bits (1185), Expect = 7.8e-130
Identity = 218/316 (68.99%), Postives = 260/316 (82.28%), Query Frame = 0

Query: 16  ELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVIHDPLADLLAGEACLR 75
           EL  PD+L ++ V+++H  I +EWDYLQ+SACQ AAGRALWKHVIHDPLA L AGE  LR
Sbjct: 28  ELVLPDVLRSEGVKKLHMSIQSEWDYLQKSACQTAAGRALWKHVIHDPLAHLFAGETHLR 87

Query: 76  NLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYR 135
           NLH KI+ DR NNARE+SGVILAVRTLWFD++I+ AL SF+G  +QVVLLGAGMD R+YR
Sbjct: 88  NLHTKIQTDRLNNAREVSGVILAVRTLWFDTRIQAALESFHGDAAQVVLLGAGMDARSYR 147

Query: 136 LSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSLNRVAADIRSSDWLEK 195
           L+CL +SDVFEVDF ++L+ KA+L++ A+ S  E    +  AKSL RVA DIR +DW E+
Sbjct: 148 LNCLNKSDVFEVDFQDVLETKASLVQAAVNSRDE---LRMTAKSLVRVAIDIRDNDWFEQ 207

Query: 196 LQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTVLLADFMNKPSTTLSN 255
           L+ +GF PE NTVWVLEG+LYY +H++AMQVL  IA+KC +T TVLLADFMNKPS TL N
Sbjct: 208 LKKSGFLPEINTVWVLEGILYYLSHTEAMQVLNLIAEKCGLTSTVLLADFMNKPSATLPN 267

Query: 256 SVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFNKIRSLPRSLQTHPDD 315
           SVFHFY DWPD LLP+LGFS  KLSQIGDPDA+FGL+HDP NLFNK+  LPR+ Q HPDD
Sbjct: 268 SVFHFYSDWPDQLLPTLGFSHVKLSQIGDPDANFGLLHDPRNLFNKLLRLPRTAQIHPDD 327

Query: 316 GKPCCRLYLVQASGSP 332
           GKPCCRLYLV+ASGSP
Sbjct: 328 GKPCCRLYLVEASGSP 340

BLAST of CmaCh12G004710 vs. TAIR 10
Match: AT5G42760.2 (Leucine carboxyl methyltransferase )

HSP 1 Score: 449.5 bits (1155), Expect = 2.4e-126
Identity = 213/313 (68.05%), Postives = 254/313 (81.15%), Query Frame = 0

Query: 16  ELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVIHDPLADLLAGEACLR 75
           EL  PD+L ++ V+++H  I +EWDYLQ+SACQ AAGRALWKHVIHDPLA L AGE  LR
Sbjct: 24  ELVLPDVLRSEGVKKLHMSIQSEWDYLQKSACQTAAGRALWKHVIHDPLAHLFAGETHLR 83

Query: 76  NLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDSQVVLLGAGMDTRAYR 135
           NLH KI+ DR NNARE+SGVILAVRTLWFD++I+ AL SF+G  +QVVLLGAGMD R+YR
Sbjct: 84  NLHTKIQTDRLNNAREVSGVILAVRTLWFDTRIQAALESFHGDAAQVVLLGAGMDARSYR 143

Query: 136 LSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSLNRVAADIRSSDWLEK 195
           L+CL +SDVFEVDF ++L+ KA+L++ A+ S  E    +  AKSL RVA DIR +DW E+
Sbjct: 144 LNCLNKSDVFEVDFQDVLETKASLVQAAVNSRDE---LRMTAKSLVRVAIDIRDNDWFEQ 203

Query: 196 LQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTVLLADFMNKPSTTLSN 255
           L+ +GF PE NTVWVLEG+LYY +H++AMQVL  IA+KC +T TVLLADFMNKPS TL N
Sbjct: 204 LKKSGFLPEINTVWVLEGILYYLSHTEAMQVLNLIAEKCGLTSTVLLADFMNKPSATLPN 263

Query: 256 SVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFNKIRSLPRSLQTHPDD 315
           SVFHFY DWPD LLP+LGFS  KLSQIGDPDA+FGL+HDP NLFNK+  LPR+ Q HPDD
Sbjct: 264 SVFHFYSDWPDQLLPTLGFSHVKLSQIGDPDANFGLLHDPRNLFNKLLRLPRTAQIHPDD 323

Query: 316 GKPCCRLYLVQAS 329
           GKPCCRLYL   S
Sbjct: 324 GKPCCRLYLFYIS 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A8LHA43.1e-1433.57Putative S-adenosyl-L-methionine-dependent methyltransferase Franean1_4929 OS=Fr... [more]
B1MGB74.0e-1434.03Putative S-adenosyl-L-methionine-dependent methyltransferase MAB_3787 OS=Mycobac... [more]
B1MEP96.9e-1431.28Putative S-adenosyl-L-methionine-dependent methyltransferase MAB_0213c OS=Mycoba... [more]
B2HD966.9e-1430.86Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_2791 OS=Mycoba... [more]
B2HLS49.9e-1336.30Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_0356 OS=Mycoba... [more]
Match NameE-valueIdentityDescription
A0A6J1KNW12.3e-198100.00uncharacterized protein LOC111496264 OS=Cucurbita maxima OX=3661 GN=LOC111496264... [more]
A0A6J1GI612.7e-19497.92uncharacterized protein LOC111454414 OS=Cucurbita moschata OX=3662 GN=LOC1114544... [more]
A0A6J1CRT33.1e-17488.46uncharacterized protein LOC111013987 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1KE322.0e-17389.25uncharacterized protein LOC111492397 OS=Cucurbita maxima OX=3661 GN=LOC111492397... [more]
A0A6J1HBI52.2e-17288.66uncharacterized protein LOC111462501 OS=Cucurbita moschata OX=3662 GN=LOC1114625... [more]
Match NameE-valueIdentityDescription
XP_023002415.14.8e-198100.00uncharacterized protein LOC111496264 [Cucurbita maxima][more]
XP_023537171.18.5e-19598.22uncharacterized protein LOC111798329 [Cucurbita pepo subsp. pepo][more]
KAG7020511.14.2e-19497.92hypothetical protein SDJN02_17196 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022951671.15.5e-19497.92uncharacterized protein LOC111454414 [Cucurbita moschata][more]
KAG6585597.14.1e-18198.42hypothetical protein SDJN03_18330, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT5G42760.17.8e-13068.99Leucine carboxyl methyltransferase [more]
AT5G42760.22.4e-12668.05Leucine carboxyl methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 38..290
e-value: 2.0E-48
score: 167.2
NoneNo IPR availablePANTHERPTHR43619:SF8LEUCINE CARBOXYL METHYLTRANSFERASEcoord: 16..326
NoneNo IPR availablePANTHERPTHR43619S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE YKTD-RELATEDcoord: 16..326
IPR011610S-adenosyl-L-methionine-dependent methyltransferase, putativeTIGRFAMTIGR00027TIGR00027coord: 50..235
e-value: 6.3E-37
score: 125.4
IPR007213Methyltransferase Ppm1/Ppm2/TcmpPFAMPF04072LCMcoord: 46..231
e-value: 1.6E-47
score: 161.9
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 43..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G004710.1CmaCh12G004710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity