CmaCh12G004380 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G004380
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionzingipain-2
LocationCma_Chr12: 2287015 .. 2291955 (+)
RNA-Seq ExpressionCmaCh12G004380
SyntenyCmaCh12G004380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TACCAAAGCCGAAACGAAATTGTTGGCAAACAGGTCAAAATCATTCTCGAAAAATCCAAAAAATCCAAACAAAATCATTCAAGTTCGACGATTTTCGTGAGTTAATTGTCTGTCAATTGCCGATTGGTTTGGGACCATTGTTCATTGAAGATCGAATAGCCAATCCTCTGGTTATTTGATAGCAGCCATCTACGGCGGCCGTGTACCGACTCGCCGGCGATTAACCAGCACCGCCACGCTTTATCTCGGCAAAGAATGGCCAACCTCAACTTCCATTTTGTGATTACCTTTCTCTTTCTTCTTCGCCATTTTTCTGCTACTTCGGATATTTCGGAGCTCTTCGAAATCTGGTGCACAGAACATGGAAAATCGTATTCCTCCGCGGAAGAAAAGCTATACAGACTCGGTGTTTTTGCCGATAACTATGAATTTGTTACTCATCACAATAATCAGGGAAATTCTTCTTATACTCTTTCTCTCAATGCTTATGCCGATATTACTCACCATGAGTTCAAGGCCGCTCGCTTGGGTCTTTCCTCTGCTTTGCGGAACTCACGGCCGGTTTCGCCGCAAGAACCCTATCTTCATCAGGATGTTCCTGAATTGTTAGATTGGAGGAAGAAAGGGGCTGTGACTGCTGTTAAGGATCAAGGAAGTTGTGGTATGTTTAATTTCTGTTTTCGATTGTTTTGTTTGGTGAAGATCTTGAATCTGATTGAACTACTCTGTTCCGTGATTTTGAATTTGATTGAAACCCTAGGACCCTAGGGTTAGTTTTGACGATCGATGCATTACAAGTGGAAATTTCTATAAATGGCTTCATGAACATGGGGTTATCTATTACAAATCCGAACAAAGAAATCATTAGAAGTGGAAATTGTGCTTCTGTCTCTTGAAACCAAACATTATTGCTTGGCCATATGGGTTTAAACATTTGTTCCTCATTCTATTTTAAGATTCTATATACAGAATTGCAGAAATATAGTTCATGTTTGAGCAAAATATAACCGCATAAGTCCACCGTGACCAGATATTATCTTCTTTGGGCTTCCCCTCCTCTGCTAAGGAGAGGTTTCCACACCCTTATAAAGAATATTTCGTTCTCCTTTCCAATTGATGTAGGATCTCACAATCCACCTCCCTTCGGGGCCAGCGTCCTCACTGACACTTGTTCCCTTCTCCAATCGATGTGGGACCCTCCAATCCACCCCCTTAGAGATCCAACGTCCTTGCTAGCACACCACCTCGTGTTCACCTCTTTCGGGGCTCAGCCTTCTTGCTGGTACATCGCTCGGTGTTTAGCTCCAATACTATTTGTAACGGCTCAAGCCCACCGGTAGCCGATATTGTTCTATTTGGACTTCCCCTCAAGGTTTTTAAAACGCGTATGCTAGGGAGAGGTTACCACACTCTTATAAAAAAATATTTTGTTCTCCTCGGGATCTCAAACAAAATATAAAGAAAAGTGTTGAAAATGTATTAAATTATGAACTATACTCTAGCTGAGTTTTGGATCTAGTGATCATCAAGGTAGTTGAGTTGTATCATCATCTCTTCATGATAAGCTTCAAGTTATGTGGAAGCTTTGCTCATCTAAATGGGAAAGAACCAATATCTTTTGTTTTCACTTTTCAGGTGCTTGCTGGTCTTTCTCAGCAACAGGAGCTATTGAAGGGATTAACCAGATTAGAACAGGGTCTCTTATCAGTGTTTCTGAACAGGAATTAATTGATTGTGACAGATCGTATAATTCTGGCTGTGGAGGAGGACTGATGGATTATGCATACCAATTTGTGATAAAAAACCATGGGATCGACACCGAAGATGATTATCCTTTTCAAGGTCGTGATGGATCGTGTCGTAAGGACAAGGTAATATAATTCATCTCTCCCTCACATCTTCCAAATACGTTCACAATATTAGTAAATCACTTTTTATGGACACAGTTTGAATGATTCACGAGCCCACCGTAAGCAAATATTGTTCTCCTTGGGTTTTCCCTTTCGGGCTTCATCTCAAGGTTTTTAAAACATCTACTAGGGAGAGGTTTCCACACCCTTATAAAGAATGTTTCGGTCTTCGTTCTCCTCATCCCCACCCCCTTCTGGGCGCAGCGTCCTCGCTGGCACTCATTCCCTCTCTCCAATCAATGTGGGACCCCCAATCCACCCCCTTCAGGGTCTAGCGTCCTTACTGGCACACTGCCTCGTGTCCACTTCACTTCGAGGCTCAGCCTTCTTGATGTGAGATTCCCCAATCCACTTCCCTTCGGTGCCCAGTGTTCTTGCTGGCATCCGCCTCATGTCCACCCCCTTTCGGGGCTCAGCCTCCTCGCTAGCACATCACTTGGTGTCTGGCTCTAATACCATTTGTAACGGCTTAAGCCCACCGCTAGCAAATATTGTCTCTTTGGGCTTTCCGTTTCATGGTTCCCTTAAAGGTTTTTAAAACGCGTCTAGTAGGGAGAGGTTTCCACACCCTTATAAAAAATGTTCCGTTCTTCTTCCCAACCGAGGTAGGATCTCACATAGAAGATGGATTCCTTACGGATAAAAAAGATTGCTTGCGCTGAAATGCATTTTGTGTCTATTGAGCTTGCAGCTAAATAGGAAGGTCGTTACCATTGATGGCTATTCGGATGTTCCTCCAAACAATGAGGAAAAATTACTGCAAGCAGTAGCAATTCAACCTGTGAGTGTTGGTATCTGTGGCAGTGAGAGAGCTTTTCAATTATATTCAAAGGTTGGTTCTATTCTCCACTAATGTTTGAAAATTCCATCATACGTGAAATTATGACTAAAATCAAGAATGGTACTTGTTTTTGTTAGGGAATTTTCTCTGGTCCATGTTCAACTTCCTTGGATCATGCTGTGTTGATTGTAGGATATGGATCAGAAAATGGTGTTGATTATTGGATCGTGAAGAACTCGTGGGGTAAACGTTGGGGAATGGATGGTTATATTCATATGCAGCGCAACAGCGGAAATTCTGAAGGCGTTTGCGGAATCAACATGCTTGCTTCATATCCAATTAAAACGAGTCCCAACCCGCCTCCCTCCCCTCCTCCAGGTCCAACAAAATGCAGTTTTCTTACTAGCTGTGCTGCTGGGGAGACCTGTTGTTGTGCAAAGGAATTTTTTGGCCTTTGCTTGTCTTGGAAATGCTGTGGTCTGAGCTCTGCTGTCTGTTGCAAGGACGGTCGTCATTGTTGCCCCTTTGATTATCCCATTTGTGATCCTCAGAGGAACCTATGCCTCAAGGTTTGTTCCTTTTCCCACTTCTTGTGATTTTGTGTTTGAGATGAAAATATCATTTTAGTGTTGGAAGTAGCTTGCCTGTGTGTTGTTTTCTTCCTTCAAACAAGTTTAATCATCTGGAAATGGATCTGTTTTCATAAGCATATGTTAGAGAAGAAAAAGAAGAGAACGAACGAGCTATAACTCGTCTCTTGTTGTGAGATCCCACATCGGTTGGAGAAGGAAACGAAACATTCTTTATAAGGGTGTGGAAACATCTCCCTAGCAAACACATTTTAAAAACCTCAAGGGGAAGGCTGGAAAGGAAAGCCTGAAAAGGATAATATCTGCTAACGGTTGGCTTGGGCTGTTACAGATGGTATCAGAGCACACTAGGCGGTGTGCTAGTGAGGATGTTGGACCCTGAAAAGGGATGGATTGTGAGATTCCACATTGATTAGAGAGGGGAATGAGTGCCAGCGAGGGCGTTAGGCCTCGAAGATGGGTGGATTGTGAGATCCTACGTCGATTGGAGAGGGGAACGAAACATTCTTTATAAGAGTGTGGAAACCTCTCCCCATTAGATGCGTTTTAAAAACCTTGAGGGAAAGTCCAAAGAGGACAATATCTGCGAACGGTGGGCTTGGGCTCTTATAGATGGTATCAAAGCACATCAGGCAGTGTACCAACGAGGACATTGGGCCCCGAAAGGGGTGGATTGTGAGATCCCACATCTATTGGAGAGGGGAATAAGTGCCAGCGAGAACGTTGTGCCCCGAAGGGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAAGGGAACGAAACATTTTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGACGCATTTTAAAACCTTGAGGGGAACCCCGAAAGTGAAAACCGAAAATGGATAATATCTGCTAGTGATGGGTTTTGACCGTTACAGTTACCATCTTTTCTTGTACCTGATTGACAATTGTCACACTCTGTCTCAATCTAATAGTTAGTAACCATACCAAACACTCACCTGTTTACAGCTGCATAGCGACACAACTTCTAACAAAAGAACATTTTCCAAGAATGCATCAAAAAATCAGTTGAAACATGTTCGAAGTCATATGTTTGAAGTGTTTCTTTGTCTATGTTCTCTCTGTTCCTTATTCTGGGCTAACAGAAATATCATCTTGGCAGAGAACGATGAACGGTACAAGAACAGAAGCACTCGAGAATCGGAGTCCTTCGGGAACATCCGGTTCTTGGAGCTCTTTATAAGGTTTGAATTCTATGAAATGGCTCCATTTGTAGAATGAATAAACGCTGCCATTCTAGCTACCACTGTATTCTACAAGCTTTTAGCTATATTTGAAGTCTAAAAAGGAGGTTCAGTCATTAGCTTTCTGCTATGGTCCTAGAGCTTCCATGGATTAGCTTTGGAAAGTTCTTACCTCGGCCGACCTCGGAGTTTTATCGGGTTCTGGCTCGTCACTTTTCTTAGGTTTCTGATCAGACTGCTGCATTTCAAGCTCTTAAAGTTGCATCTTACTCGATGCATAGATTTGTTCTTGAATATTATGGATTGGTTTGGATTTGAATGTTTGTGATTAGTTGTAAGAGAAATTTGGTTCATATATTGCTGCCTCATATGCTCCAACGTAGTTTTTTTTATGCTTTTCTGTTACTAAATCTTTAGGACGGAGTTAG

mRNA sequence

TACCAAAGCCGAAACGAAATTGTTGGCAAACAGGTCAAAATCATTCTCGAAAAATCCAAAAAATCCAAACAAAATCATTCAAGTTCGACGATTTTCGTGAGTTAATTGTCTGTCAATTGCCGATTGGTTTGGGACCATTGTTCATTGAAGATCGAATAGCCAATCCTCTGGTTATTTGATAGCAGCCATCTACGGCGGCCGTGTACCGACTCGCCGGCGATTAACCAGCACCGCCACGCTTTATCTCGGCAAAGAATGGCCAACCTCAACTTCCATTTTGTGATTACCTTTCTCTTTCTTCTTCGCCATTTTTCTGCTACTTCGGATATTTCGGAGCTCTTCGAAATCTGGTGCACAGAACATGGAAAATCGTATTCCTCCGCGGAAGAAAAGCTATACAGACTCGGTGTTTTTGCCGATAACTATGAATTTGTTACTCATCACAATAATCAGGGAAATTCTTCTTATACTCTTTCTCTCAATGCTTATGCCGATATTACTCACCATGAGTTCAAGGCCGCTCGCTTGGGTCTTTCCTCTGCTTTGCGGAACTCACGGCCGGTTTCGCCGCAAGAACCCTATCTTCATCAGGATGTTCCTGAATTGTTAGATTGGAGGAAGAAAGGGGCTGTGACTGCTGTTAAGGATCAAGGAAGTTGTGGTGCTTGCTGGTCTTTCTCAGCAACAGGAGCTATTGAAGGGATTAACCAGATTAGAACAGGGTCTCTTATCAGTGTTTCTGAACAGGAATTAATTGATTGTGACAGATCGTATAATTCTGGCTGTGGAGGAGGACTGATGGATTATGCATACCAATTTGTGATAAAAAACCATGGGATCGACACCGAAGATGATTATCCTTTTCAAGGTCGTGATGGATCGTGTCGTAAGGACAAGGTCGTTACCATTGATGGCTATTCGGATGTTCCTCCAAACAATGAGGAAAAATTACTGCAAGCAGTAGCAATTCAACCTGTGAGTGTTGGTATCTGTGGCAGTGAGAGAGCTTTTCAATTATATTCAAAGGGAATTTTCTCTGGTCCATGTTCAACTTCCTTGGATCATGCTGTGTTGATTGTAGGATATGGATCAGAAAATGGTGTTGATTATTGGATCGTGAAGAACTCGTGGGGTAAACGTTGGGGAATGGATGGTTATATTCATATGCAGCGCAACAGCGGAAATTCTGAAGGCGTTTGCGGAATCAACATGCTTGCTTCATATCCAATTAAAACGAGTCCCAACCCGCCTCCCTCCCCTCCTCCAGGTCCAACAAAATGCAGTTTTCTTACTAGCTGTGCTGCTGGGGAGACCTGTTGTTGTGCAAAGGAATTTTTTGGCCTTTGCTTGTCTTGGAAATGCTGTGGTCTGAGCTCTGCTGTCTGTTGCAAGGACGGTCGTCATTGTTGCCCCTTTGATTATCCCATTTGTGATCCTCAGAGGAACCTATGCCTCAAGAGAACGATGAACGGTACAAGAACAGAAGCACTCGAGAATCGGAGTCCTTCGGGAACATCCGGACGGAGTTAG

Coding sequence (CDS)

ATGGCCAACCTCAACTTCCATTTTGTGATTACCTTTCTCTTTCTTCTTCGCCATTTTTCTGCTACTTCGGATATTTCGGAGCTCTTCGAAATCTGGTGCACAGAACATGGAAAATCGTATTCCTCCGCGGAAGAAAAGCTATACAGACTCGGTGTTTTTGCCGATAACTATGAATTTGTTACTCATCACAATAATCAGGGAAATTCTTCTTATACTCTTTCTCTCAATGCTTATGCCGATATTACTCACCATGAGTTCAAGGCCGCTCGCTTGGGTCTTTCCTCTGCTTTGCGGAACTCACGGCCGGTTTCGCCGCAAGAACCCTATCTTCATCAGGATGTTCCTGAATTGTTAGATTGGAGGAAGAAAGGGGCTGTGACTGCTGTTAAGGATCAAGGAAGTTGTGGTGCTTGCTGGTCTTTCTCAGCAACAGGAGCTATTGAAGGGATTAACCAGATTAGAACAGGGTCTCTTATCAGTGTTTCTGAACAGGAATTAATTGATTGTGACAGATCGTATAATTCTGGCTGTGGAGGAGGACTGATGGATTATGCATACCAATTTGTGATAAAAAACCATGGGATCGACACCGAAGATGATTATCCTTTTCAAGGTCGTGATGGATCGTGTCGTAAGGACAAGGTCGTTACCATTGATGGCTATTCGGATGTTCCTCCAAACAATGAGGAAAAATTACTGCAAGCAGTAGCAATTCAACCTGTGAGTGTTGGTATCTGTGGCAGTGAGAGAGCTTTTCAATTATATTCAAAGGGAATTTTCTCTGGTCCATGTTCAACTTCCTTGGATCATGCTGTGTTGATTGTAGGATATGGATCAGAAAATGGTGTTGATTATTGGATCGTGAAGAACTCGTGGGGTAAACGTTGGGGAATGGATGGTTATATTCATATGCAGCGCAACAGCGGAAATTCTGAAGGCGTTTGCGGAATCAACATGCTTGCTTCATATCCAATTAAAACGAGTCCCAACCCGCCTCCCTCCCCTCCTCCAGGTCCAACAAAATGCAGTTTTCTTACTAGCTGTGCTGCTGGGGAGACCTGTTGTTGTGCAAAGGAATTTTTTGGCCTTTGCTTGTCTTGGAAATGCTGTGGTCTGAGCTCTGCTGTCTGTTGCAAGGACGGTCGTCATTGTTGCCCCTTTGATTATCCCATTTGTGATCCTCAGAGGAACCTATGCCTCAAGAGAACGATGAACGGTACAAGAACAGAAGCACTCGAGAATCGGAGTCCTTCGGGAACATCCGGACGGAGTTAG

Protein sequence

MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDWRKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKDKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCCAKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRSPSGTSGRS
Homology
BLAST of CmaCh12G004380 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.4e-117
Identity = 208/411 (50.61%), Postives = 271/411 (65.94%), Query Frame = 0

Query: 23  SDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHNNQGNSSYTLSLNAYADIT 82
           ++   ++E W  E+ K+Y+   EK  R  +F DN +FV  H++  N +Y + L  +AD+T
Sbjct: 37  AEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLT 96

Query: 83  HHEFKAARLGLSSALRNSRPVSPQEPYLHQ---DVPELLDWRKKGAVTAVKDQGSCGACW 142
           + EF+A  + L S +  +R     E YL++    +P+ +DWR KGAV  VKDQGSCG+CW
Sbjct: 97  NDEFRA--IYLRSKMERTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCW 156

Query: 143 SFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHGIDTED 202
           +FSA GA+EGINQI+TG LIS+SEQEL+DCD SYN GCGGGLMDYA++F+I+N GIDTE+
Sbjct: 157 AFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEE 216

Query: 203 DYPFQGRD-GSCRKDK----VVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQL 262
           DYP+   D   C  DK    VVTIDGY DVP N+E+ L +A+A QP+SV I    RAFQL
Sbjct: 217 DYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQL 276

Query: 263 YSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGV 322
           Y+ G+F+G C TSLDH V+ VGYGSE G DYWIV+NSWG  WG  GY  ++RN   S G 
Sbjct: 277 YTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGK 336

Query: 323 CGINMLASYPIKTSPNPPPSPP-PGPTKCSFLTSCAAGETCCCAKEFFGLCLSWKCCGLS 382
           CG+ M+ASYP K+S + PP PP P P  C    +C A  TCCC  E+ G C SW CC   
Sbjct: 337 CGVAMMASYPTKSSGSNPPKPPAPSPVVCDKSNTCPAKSTCCCLYEYNGKCYSWGCCPYE 396

Query: 383 SAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRSPSGTSGRS 425
           SA CC DG  CCP  YP+CD + N C  +  +    +AL  R P+  + +S
Sbjct: 397 SATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKAL-TRGPAIATTKS 444

BLAST of CmaCh12G004380 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 422.5 bits (1085), Expect = 5.5e-117
Identity = 210/402 (52.24%), Postives = 258/402 (64.18%), Query Frame = 0

Query: 28  LFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHN---NQGNSSYTLSLNAYADITHH 87
           L+  W  EHGKSY++  E+  R   F DN  ++  HN   + G  S+ L LN +AD+T+ 
Sbjct: 39  LYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVHSFRLGLNRFADLTNE 98

Query: 88  EFKAARLGLSSALRNSRPVSPQEPYLHQD---VPELLDWRKKGAVTAVKDQGSCGACWSF 147
           E++   LGL +  R  R VS  + YL  D   +PE +DWR KGAV  +KDQG CG+CW+F
Sbjct: 99  EYRDTYLGLRNKPRRERKVS--DRYLAADNEALPESVDWRTKGAVAEIKDQGGCGSCWAF 158

Query: 148 SATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHGIDTEDDY 207
           SA  A+EGINQI TG LIS+SEQEL+DCD SYN GC GGLMDYA+ F+I N GIDTEDDY
Sbjct: 159 SAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAFDFIINNGGIDTEDDY 218

Query: 208 PFQGRDGSC----RKDKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQLYSK 267
           P++G+D  C    +  KVVTID Y DV PN+E  L +AVA QPVSV I    RAFQLYS 
Sbjct: 219 PYKGKDERCDVNRKNAKVVTIDSYEDVTPNSETSLQKAVANQPVSVAIEAGGRAFQLYSS 278

Query: 268 GIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGVCGI 327
           GIF+G C T+LDH V  VGYG+ENG DYWIV+NSWGK WG  GY+ M+RN   S G CGI
Sbjct: 279 GIFTGKCGTALDHGVAAVGYGTENGKDYWIVRNSWGKSWGESGYVRMERNIKASSGKCGI 338

Query: 328 NMLASYPIKTSPNP------PPSPPPGPTKCSFLTSCAAGETCCCAKEFFGLCLSWKCCG 387
            +  SYP+K   NP      PPSP P PT C    +C    TCCC  E+   C +W CC 
Sbjct: 339 AVEPSYPLKKGENPPNPGPTPPSPTPPPTVCDNYYTCPDSTTCCCIYEYGKYCYAWGCCP 398

Query: 388 LSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALE 414
           L  A CC D   CCP +YPIC+ Q+  CL    +    +AL+
Sbjct: 399 LEGATCCDDHYSCCPHEYPICNVQQGTCLMAKDSPLAVKALK 438

BLAST of CmaCh12G004380 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.5e-116
Identity = 207/416 (49.76%), Postives = 266/416 (63.94%), Query Frame = 0

Query: 23  SDISELFEIWCTEHGK--SYSSAEEKLYRLGVFADNYEFVTHHNNQGNSSYTLSLNAYAD 82
           +++  ++E W  +HGK  S +S  EK  R  +F DN  FV  HN + N SY L L  +AD
Sbjct: 44  AEVMSIYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLRFVDEHNEK-NLSYRLGLTRFAD 103

Query: 83  ITHHEFKAARLGLSSALRNSRPVSPQ-EPYLHQDVPELLDWRKKGAVTAVKDQGSCGACW 142
           +T+ E+++  LG     +  R  S + E  +  ++PE +DWRKKGAV  VKDQG CG+CW
Sbjct: 104 LTNDEYRSKYLGAKMEKKGERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCW 163

Query: 143 SFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHGIDTED 202
           +FS  GA+EGINQI TG LI++SEQEL+DCD SYN GC GGLMDYA++F+IKN GIDT+ 
Sbjct: 164 AFSTIGAVEGINQIVTGDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDK 223

Query: 203 DYPFQGRDGSC----RKDKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQLY 262
           DYP++G DG+C    +  KVVTID Y DVP  +EE L +AVA QP+S+ I    RAFQLY
Sbjct: 224 DYPYKGVDGTCDQIRKNAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLY 283

Query: 263 SKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGVC 322
             GIF G C T LDH V+ VGYG+ENG DYWIV+NSWGK WG  GY+ M RN  +S G C
Sbjct: 284 DSGIFDGSCGTQLDHGVVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKC 343

Query: 323 GINMLASYPIKTSPNP------PPSPPPGPTKCSFLTSCAAGETCCCAKEFFGLCLSWKC 382
           GI +  SYPIK   NP      PPSP   PT+C    +C    TCCC  E+   C +W C
Sbjct: 344 GIAIEPSYPIKNGENPPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGC 403

Query: 383 CGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENR--SPSGTSGR 424
           C L +A CC D   CCP +YP+CD  +  CL    +    +AL+ +  +P  + GR
Sbjct: 404 CPLEAATCCDDNYSCCPHEYPVCDLDQGTCLLSKNSPFSVKALKRKPATPFWSQGR 458

BLAST of CmaCh12G004380 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 8.7e-115
Identity = 205/410 (50.00%), Postives = 256/410 (62.44%), Query Frame = 0

Query: 23  SDISELFEIWCTEHGKSYSS----AEEKLYRLGVFADNYEFVTHHNNQGNSSYTLSLNAY 82
           S++  ++E W  EHGK   +      EK  R  +F DN  F+  HN + N SY L L  +
Sbjct: 44  SEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTK-NLSYKLGLTRF 103

Query: 83  ADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDWRKKGAVTAVKDQGSCGAC 142
           AD+T+ E+++  LG     R  +     +  +   +P+ +DWRK+GAV  VKDQGSCG+C
Sbjct: 104 ADLTNEEYRSMYLGAKPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSC 163

Query: 143 WSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHGIDTE 202
           W+FS  GA+EGIN+I TG LIS+SEQEL+DCD SYN GC GGLMDYA++F+IKN GIDTE
Sbjct: 164 WAFSTIGAVEGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTE 223

Query: 203 DDYPFQGRDGSC----RKDKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQL 262
            DYP++  DG C    +  KVVTID Y DVP N+E  L +A+A QP+SV I    RAFQL
Sbjct: 224 ADYPYKAADGRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQL 283

Query: 263 YSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGV 322
           YS G+F G C T LDH V+ VGYG+ENG DYWIV+NSWG RWG  GYI M RN     G 
Sbjct: 284 YSSGVFDGLCGTELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGK 343

Query: 323 CGINMLASYPIKTSPNP------PPSPPPGPTKCSFLTSCAAGETCCCAKEFFGLCLSWK 382
           CGI M ASYPIK   NP      PPSP   PT C    SC    TCCC  ++   C  W 
Sbjct: 344 CGIAMEASYPIKKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWG 403

Query: 383 CCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRSPS 419
           CC L +A CC D   CCP +YP+CD  R  CL    +    +AL+ R+P+
Sbjct: 404 CCPLEAATCCDDNSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALK-RTPA 451

BLAST of CmaCh12G004380 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 383.6 bits (984), Expect = 2.8e-105
Identity = 195/395 (49.37%), Postives = 251/395 (63.54%), Query Frame = 0

Query: 29  FEIWCTEHGKSYSSA--EEKLYRLGVFADNYEFVTHHNNQGN--SSYTLSLNAYADITHH 88
           +++W  E+G    +A   E   R  VF DN +FV  HN + +    + L +N +AD+T+ 
Sbjct: 52  YDLWLAENGGGSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNE 111

Query: 89  EFKAARLGLSSALRNSRPVSPQEPYLH---QDVPELLDWRKKGAVTAVKDQGSCGACWSF 148
           EF+A  LG   A R+    +  E Y H   +++PE +DWR+KGAV  VK+QG CG+CW+F
Sbjct: 112 EFRATFLGAKVAERSR---AAGERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAF 171

Query: 149 SATGAIEGINQIRTGSLISVSEQELIDCD-RSYNSGCGGGLMDYAYQFVIKNHGIDTEDD 208
           SA   +E INQ+ TG +I++SEQEL++C     NSGC GGLMD A+ F+IKN GIDTEDD
Sbjct: 172 SAVSTVESINQLVTGEMITLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDD 231

Query: 209 YPFQGRDGSC----RKDKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQLYS 268
           YP++  DG C       KVV+IDG+ DVP N+E+ L +AVA QPVSV I    R FQLY 
Sbjct: 232 YPYKAVDGKCDINRENAKVVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYH 291

Query: 269 KGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGVCG 328
            G+FSG C TSLDH V+ VGYG++NG DYWIV+NSWG +WG  GY+ M+RN   + G CG
Sbjct: 292 SGVFSGRCGTSLDHGVVAVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNINVTTGKCG 351

Query: 329 INMLASYPIKTSPNPP---PSPPPGPTK---------CSFLTSCAAGETCCCAKEFFGLC 388
           I M+ASYP K+  NPP   P+PP  PT          C    SC AG TCCCA  F  LC
Sbjct: 352 IAMMASYPTKSGANPPKPSPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLC 411

Query: 389 LSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLC 400
           L W CC +  A CCKD   CCP DYP+C+ +   C
Sbjct: 412 LVWGCCPVEGATCCKDHASCCPPDYPVCNTRAGTC 443

BLAST of CmaCh12G004380 vs. ExPASy TrEMBL
Match: A0A6J1KL32 (zingipain-2 OS=Cucurbita maxima OX=3661 GN=LOC111496216 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 2.7e-252
Identity = 422/426 (99.06%), Postives = 422/426 (99.06%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV
Sbjct: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW
Sbjct: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD----KVVTIDGYSDVPPNNEEKLLQAV 240
           LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD    KVVTIDGYSDVPPNNEEKLLQAV
Sbjct: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKDKLNRKVVTIDGYSDVPPNNEEKLLQAV 240

Query: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300
           AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW
Sbjct: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300

Query: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360
           GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC
Sbjct: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360

Query: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420
           AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS
Sbjct: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420

Query: 421 PSGTSG 423
           PSGTSG
Sbjct: 421 PSGTSG 426

BLAST of CmaCh12G004380 vs. ExPASy TrEMBL
Match: A0A6J1GH92 (zingipain-2 OS=Cucurbita moschata OX=3662 GN=LOC111454188 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 6.9e-248
Identity = 415/426 (97.42%), Postives = 418/426 (98.12%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           MANL+FHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV
Sbjct: 1   MANLSFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALR+SRPVSPQEPYLH+DVPE LDW
Sbjct: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRSSRPVSPQEPYLHRDVPESLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD----KVVTIDGYSDVPPNNEEKLLQAV 240
           LMDYAYQFVIKNHGIDTEDDYPFQGRDGSC KD    KVVTIDGYSDVPPNNEEKLLQAV
Sbjct: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCHKDKLNRKVVTIDGYSDVPPNNEEKLLQAV 240

Query: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300
           AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW
Sbjct: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300

Query: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360
           GMDGYIHMQRNSGNSEGVCGINMLASYP KTSPNPPPSPPPGPTKCSFLTSCAAGETCCC
Sbjct: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPTKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360

Query: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420
           AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICD QRNLCLKRTMNGTRTEALENRS
Sbjct: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDTQRNLCLKRTMNGTRTEALENRS 420

Query: 421 PSGTSG 423
           PSGTSG
Sbjct: 421 PSGTSG 426

BLAST of CmaCh12G004380 vs. ExPASy TrEMBL
Match: A0A1S3BBY8 (zingipain-2 OS=Cucumis melo OX=3656 GN=LOC103488009 PE=3 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.3e-222
Identity = 372/426 (87.32%), Postives = 387/426 (90.85%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           M N  FHF+  FL   R   ATS++SELFEIWCTEHGKSYSSAEEKLYRL VFADNYEFV
Sbjct: 1   MGNFAFHFLTLFLLFFRPLFATSNVSELFEIWCTEHGKSYSSAEEKLYRLSVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNN GNSSYTLSLN+YAD+THHEFK +RLG S ALRN RPV PQEP L +DVP+ LDW
Sbjct: 61  THHNNLGNSSYTLSLNSYADLTHHEFKVSRLGFSPALRNFRPVLPQEPSLPRDVPDSLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSCGACWSFSATGAIEGINQI TGSLISVSEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIMTGSLISVSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKDK----VVTIDGYSDVPPNNEEKLLQAV 240
           LMDYAYQFVI NHGIDTEDDYP+QGRDGSCRKDK    VVTIDGY+D+PPN+E KLLQAV
Sbjct: 181 LMDYAYQFVISNHGIDTEDDYPYQGRDGSCRKDKLQRNVVTIDGYTDIPPNDEGKLLQAV 240

Query: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300
           A QPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGK W
Sbjct: 241 AAQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSW 300

Query: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360
           GMDGY+HMQRNSGNSEGVCGIN LASYP KTSPNPPPSPPPGPTKCS LTSCAAGETCCC
Sbjct: 301 GMDGYMHMQRNSGNSEGVCGINKLASYPTKTSPNPPPSPPPGPTKCSILTSCAAGETCCC 360

Query: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420
           AK+F GLCLSWKCCGLSSAVCCKDGRHCCPFDYPICD  RNLCLKRTMNGTR E LENRS
Sbjct: 361 AKKFLGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDTDRNLCLKRTMNGTRMEVLENRS 420

Query: 421 PSGTSG 423
            SG+SG
Sbjct: 421 SSGSSG 426

BLAST of CmaCh12G004380 vs. ExPASy TrEMBL
Match: A0A5A7VC45 (Zingipain-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005350 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.2e-220
Identity = 371/426 (87.09%), Postives = 386/426 (90.61%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           M N  FHF+  FL   R   ATS++SELFEIWCTEHGKSYSSAEEKLYRL VFADNYEFV
Sbjct: 1   MGNFAFHFLTLFLLFFRPLFATSNVSELFEIWCTEHGKSYSSAEEKLYRLSVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNN GNSSYTLSLN+YAD+THHEFK +RLG S ALRN RPV PQEP L +DVP+ LDW
Sbjct: 61  THHNNLGNSSYTLSLNSYADLTHHEFKVSRLGFSPALRNFRPVLPQEPSLPRDVPDSLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSC ACWSFSATGAIEGINQI TGSLISVSEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSC-ACWSFSATGAIEGINQIMTGSLISVSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKDK----VVTIDGYSDVPPNNEEKLLQAV 240
           LMDYAYQFVI NHGIDTEDDYP+QGRDGSCRKDK    VVTIDGY+D+PPN+E KLLQAV
Sbjct: 181 LMDYAYQFVISNHGIDTEDDYPYQGRDGSCRKDKLQRNVVTIDGYTDIPPNDEGKLLQAV 240

Query: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300
           A QPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGK W
Sbjct: 241 AAQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSW 300

Query: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360
           GMDGY+HMQRNSGNSEGVCGIN LASYP KTSPNPPPSPPPGPTKCS LTSCAAGETCCC
Sbjct: 301 GMDGYMHMQRNSGNSEGVCGINKLASYPTKTSPNPPPSPPPGPTKCSILTSCAAGETCCC 360

Query: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420
           AK+F GLCLSWKCCGLSSAVCCKDGRHCCPFDYPICD  RNLCLKRTMNGTR E LENRS
Sbjct: 361 AKKFLGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDTDRNLCLKRTMNGTRMEVLENRS 420

Query: 421 PSGTSG 423
            SG+SG
Sbjct: 421 SSGSSG 425

BLAST of CmaCh12G004380 vs. ExPASy TrEMBL
Match: A0A0A0LNP7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G362510 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.1e-220
Identity = 368/426 (86.38%), Postives = 388/426 (91.08%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           M N  FHF+  FL L R  SATS++SELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV
Sbjct: 1   MGNYAFHFLTLFLLLFRPLSATSNVSELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNN  NSSYTLSLN+YAD+THHEFK +RLG S ALRN RPV PQEP L +DVP+ LDW
Sbjct: 61  THHNNLDNSSYTLSLNSYADLTHHEFKVSRLGFSPALRNFRPVLPQEPSLPRDVPDSLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSCGACWSFSATGA+EGINQI TGSLIS+SEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSCGACWSFSATGAMEGINQIMTGSLISLSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKDK----VVTIDGYSDVPPNNEEKLLQAV 240
           LMDYAYQFVI NHGIDTE+DYP+Q RDGSCRKDK    VVTIDGY+D+P N+E KLLQAV
Sbjct: 181 LMDYAYQFVISNHGIDTENDYPYQARDGSCRKDKLQRNVVTIDGYADIPSNDEGKLLQAV 240

Query: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300
           A QPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGK W
Sbjct: 241 AAQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSW 300

Query: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360
           GMDGY+HMQRNSGNSEGVCGIN LASYP KT+PNPPPSPPPGPTKCS LTSCAAGETCCC
Sbjct: 301 GMDGYMHMQRNSGNSEGVCGINKLASYPTKTNPNPPPSPPPGPTKCSILTSCAAGETCCC 360

Query: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420
           AK+F GLCLSWKCCGLSSAVCCKDGRHCCPFDYPICD  RNLCLK+TMNGTRTE LENRS
Sbjct: 361 AKKFLGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDTDRNLCLKQTMNGTRTEILENRS 420

Query: 421 PSGTSG 423
            SG+SG
Sbjct: 421 SSGSSG 426

BLAST of CmaCh12G004380 vs. NCBI nr
Match: XP_023002351.1 (zingipain-2 [Cucurbita maxima])

HSP 1 Score: 880.6 bits (2274), Expect = 5.6e-252
Identity = 422/426 (99.06%), Postives = 422/426 (99.06%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV
Sbjct: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW
Sbjct: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD----KVVTIDGYSDVPPNNEEKLLQAV 240
           LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD    KVVTIDGYSDVPPNNEEKLLQAV
Sbjct: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKDKLNRKVVTIDGYSDVPPNNEEKLLQAV 240

Query: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300
           AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW
Sbjct: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300

Query: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360
           GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC
Sbjct: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360

Query: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420
           AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS
Sbjct: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420

Query: 421 PSGTSG 423
           PSGTSG
Sbjct: 421 PSGTSG 426

BLAST of CmaCh12G004380 vs. NCBI nr
Match: XP_022951321.1 (zingipain-2 [Cucurbita moschata])

HSP 1 Score: 865.9 bits (2236), Expect = 1.4e-247
Identity = 415/426 (97.42%), Postives = 418/426 (98.12%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           MANL+FHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV
Sbjct: 1   MANLSFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALR+SRPVSPQEPYLH+DVPE LDW
Sbjct: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRSSRPVSPQEPYLHRDVPESLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD----KVVTIDGYSDVPPNNEEKLLQAV 240
           LMDYAYQFVIKNHGIDTEDDYPFQGRDGSC KD    KVVTIDGYSDVPPNNEEKLLQAV
Sbjct: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCHKDKLNRKVVTIDGYSDVPPNNEEKLLQAV 240

Query: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300
           AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW
Sbjct: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300

Query: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360
           GMDGYIHMQRNSGNSEGVCGINMLASYP KTSPNPPPSPPPGPTKCSFLTSCAAGETCCC
Sbjct: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPTKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360

Query: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420
           AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICD QRNLCLKRTMNGTRTEALENRS
Sbjct: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDTQRNLCLKRTMNGTRTEALENRS 420

Query: 421 PSGTSG 423
           PSGTSG
Sbjct: 421 PSGTSG 426

BLAST of CmaCh12G004380 vs. NCBI nr
Match: XP_023538152.1 (zingipain-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 864.8 bits (2233), Expect = 3.2e-247
Identity = 415/426 (97.42%), Postives = 418/426 (98.12%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           MANL+FHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV
Sbjct: 1   MANLSFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNNQ NSSYTLSLNAYADITHHEFKAARLGLSSALR+SRPVSPQEPYLH+DVPE LDW
Sbjct: 61  THHNNQENSSYTLSLNAYADITHHEFKAARLGLSSALRSSRPVSPQEPYLHRDVPESLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD----KVVTIDGYSDVPPNNEEKLLQAV 240
           LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD    KVVTIDGYSDVPPNNEEKLLQAV
Sbjct: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKDKLNRKVVTIDGYSDVPPNNEEKLLQAV 240

Query: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300
           AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW
Sbjct: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300

Query: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360
           GMDGYIHMQRNSGNSEGVCGINMLASYP KTSPNPPPSPPPGPTKCSFLTSCAAGETCCC
Sbjct: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPTKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360

Query: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420
           AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICD QRNLCLKRTMNGTRTEALENRS
Sbjct: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDTQRNLCLKRTMNGTRTEALENRS 420

Query: 421 PSGTSG 423
           PSGTSG
Sbjct: 421 PSGTSG 426

BLAST of CmaCh12G004380 vs. NCBI nr
Match: KAG6585568.1 (putative cysteine protease RD21C, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 861.3 bits (2224), Expect = 3.5e-246
Identity = 414/427 (96.96%), Postives = 417/427 (97.66%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           MANL+FHFVI FLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV
Sbjct: 1   MANLSFHFVIAFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALR+S PVSPQEPYLH+DVPE LDW
Sbjct: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRSSWPVSPQEPYLHRDVPESLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD----KVVTIDGYSDVPPNNEEKLLQAV 240
           LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKD    KVVTIDGYSDVPPNNEEKLLQAV
Sbjct: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKDKLNRKVVTIDGYSDVPPNNEEKLLQAV 240

Query: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300
           AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW
Sbjct: 241 AIQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRW 300

Query: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360
           GMDGYIHMQRNSGNSEGVCGINMLASYP KTSPNPPPSPPPGPTKCSFLTSCAAGETCCC
Sbjct: 301 GMDGYIHMQRNSGNSEGVCGINMLASYPTKTSPNPPPSPPPGPTKCSFLTSCAAGETCCC 360

Query: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRS 420
           AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICD QRNLCLKRTMNGTRTEALENRS
Sbjct: 361 AKEFFGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDTQRNLCLKRTMNGTRTEALENRS 420

Query: 421 PSGTSGR 424
           PSGTS R
Sbjct: 421 PSGTSVR 427

BLAST of CmaCh12G004380 vs. NCBI nr
Match: KAG7020481.1 (putative cysteine protease RD21C [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 808.5 bits (2087), Expect = 2.7e-230
Identity = 390/422 (92.42%), Postives = 394/422 (93.36%), Query Frame = 0

Query: 1   MANLNFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60
           MANL+FHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV
Sbjct: 1   MANLSFHFVITFLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFV 60

Query: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDW 120
           THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALR+SRPVSPQEPYLH+DVPE LDW
Sbjct: 61  THHNNQGNSSYTLSLNAYADITHHEFKAARLGLSSALRSSRPVSPQEPYLHRDVPESLDW 120

Query: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180
           RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG
Sbjct: 121 RKKGAVTAVKDQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGG 180

Query: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSCRKDKVVTIDGYSDVPPNNEEKLLQAVAIQP 240
           LMDYAYQFVIKNHGIDTEDDYPFQGRDGSC                         +AIQP
Sbjct: 181 LMDYAYQFVIKNHGIDTEDDYPFQGRDGSC-------------------------LAIQP 240

Query: 241 VSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDG 300
           VSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDG
Sbjct: 241 VSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDG 300

Query: 301 YIHMQRNSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCCAKEF 360
           YIHMQRNSGNSEGVCGINMLASYP KTSPNPPPSPPPGPTKCSFLTSCAAGETCCCAKEF
Sbjct: 301 YIHMQRNSGNSEGVCGINMLASYPTKTSPNPPPSPPPGPTKCSFLTSCAAGETCCCAKEF 360

Query: 361 FGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRSPSGT 420
           FGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICD QRNLCLKRTMNGTRTEALENRSPSGT
Sbjct: 361 FGLCLSWKCCGLSSAVCCKDGRHCCPFDYPICDTQRNLCLKRTMNGTRTEALENRSPSGT 397

Query: 421 SG 423
           SG
Sbjct: 421 SG 397

BLAST of CmaCh12G004380 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 607.8 bits (1566), Expect = 6.5e-174
Identity = 289/417 (69.30%), Postives = 338/417 (81.06%), Query Frame = 0

Query: 12  FLFLLRHFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHNNQGNSSY 71
           FL L+   S++ DISELF+ WC +HGK+Y S EE+  R+ +F DN++FVT HN   N++Y
Sbjct: 15  FLLLVSSSSSSDDISELFDDWCQKHGKTYGSEEERQQRIQIFKDNHDFVTQHNLITNATY 74

Query: 72  TLSLNAYADITHHEFKAARLGLS-SALRNSRPVSPQEPYLHQDVPELLDWRKKGAVTAVK 131
           +LSLNA+AD+THHEFKA+RLGLS SA         Q       VP+ +DWRKKGAVT VK
Sbjct: 75  SLSLNAFADLTHHEFKASRLGLSVSAPSVIMASKGQSLGGSVKVPDSVDWRKKGAVTNVK 134

Query: 132 DQGSCGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVI 191
           DQGSCGACWSFSATGA+EGINQI TG LIS+SEQELIDCD+SYN+GC GGLMDYA++FVI
Sbjct: 135 DQGSCGACWSFSATGAMEGINQIVTGDLISLSEQELIDCDKSYNAGCNGGLMDYAFEFVI 194

Query: 192 KNHGIDTEDDYPFQGRDGSCRKD----KVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGIC 251
           KNHGIDTE DYP+Q RDG+C+KD    KVVTID Y+ V  N+E+ L++AVA QPVSVGIC
Sbjct: 195 KNHGIDTEKDYPYQERDGTCKKDKLKQKVVTIDSYAGVKSNDEKALMEAVAAQPVSVGIC 254

Query: 252 GSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQR 311
           GSERAFQLYS GIFSGPCSTSLDHAVLIVGYGS+NGVDYWIVKNSWGK WGMDG++HMQR
Sbjct: 255 GSERAFQLYSSGIFSGPCSTSLDHAVLIVGYGSQNGVDYWIVKNSWGKSWGMDGFMHMQR 314

Query: 312 NSGNSEGVCGINMLASYPIKTSPNPPPSPPPGPTKCSFLTSCAAGETCCCAKEFFGLCLS 371
           N+ NS+GVCGINMLASYPIKT PNPPP  PPGPTKC+  T C++GETCCCA+E FGLC S
Sbjct: 315 NTENSDGVCGINMLASYPIKTHPNPPPPSPPGPTKCNLFTYCSSGETCCCARELFGLCFS 374

Query: 372 WKCCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRSPSGTSGR 424
           WKCC + SAVCCKDGRHCCP DYP+CD  R+LCLK+T N T  +    ++ S   GR
Sbjct: 375 WKCCEIESAVCCKDGRHCCPHDYPVCDTTRSLCLKKTGNFTAIKPFWKKNSSKQLGR 431

BLAST of CmaCh12G004380 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 424.5 bits (1090), Expect = 1.0e-118
Identity = 208/411 (50.61%), Postives = 271/411 (65.94%), Query Frame = 0

Query: 23  SDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHNNQGNSSYTLSLNAYADIT 82
           ++   ++E W  E+ K+Y+   EK  R  +F DN +FV  H++  N +Y + L  +AD+T
Sbjct: 37  AEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLT 96

Query: 83  HHEFKAARLGLSSALRNSRPVSPQEPYLHQ---DVPELLDWRKKGAVTAVKDQGSCGACW 142
           + EF+A  + L S +  +R     E YL++    +P+ +DWR KGAV  VKDQGSCG+CW
Sbjct: 97  NDEFRA--IYLRSKMERTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCW 156

Query: 143 SFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHGIDTED 202
           +FSA GA+EGINQI+TG LIS+SEQEL+DCD SYN GCGGGLMDYA++F+I+N GIDTE+
Sbjct: 157 AFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEE 216

Query: 203 DYPFQGRD-GSCRKDK----VVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQL 262
           DYP+   D   C  DK    VVTIDGY DVP N+E+ L +A+A QP+SV I    RAFQL
Sbjct: 217 DYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQL 276

Query: 263 YSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGV 322
           Y+ G+F+G C TSLDH V+ VGYGSE G DYWIV+NSWG  WG  GY  ++RN   S G 
Sbjct: 277 YTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGK 336

Query: 323 CGINMLASYPIKTSPNPPPSPP-PGPTKCSFLTSCAAGETCCCAKEFFGLCLSWKCCGLS 382
           CG+ M+ASYP K+S + PP PP P P  C    +C A  TCCC  E+ G C SW CC   
Sbjct: 337 CGVAMMASYPTKSSGSNPPKPPAPSPVVCDKSNTCPAKSTCCCLYEYNGKCYSWGCCPYE 396

Query: 383 SAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRSPSGTSGRS 425
           SA CC DG  CCP  YP+CD + N C  +  +    +AL  R P+  + +S
Sbjct: 397 SATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKAL-TRGPAIATTKS 444

BLAST of CmaCh12G004380 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 419.9 bits (1078), Expect = 2.5e-117
Identity = 207/416 (49.76%), Postives = 266/416 (63.94%), Query Frame = 0

Query: 23  SDISELFEIWCTEHGK--SYSSAEEKLYRLGVFADNYEFVTHHNNQGNSSYTLSLNAYAD 82
           +++  ++E W  +HGK  S +S  EK  R  +F DN  FV  HN + N SY L L  +AD
Sbjct: 44  AEVMSIYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLRFVDEHNEK-NLSYRLGLTRFAD 103

Query: 83  ITHHEFKAARLGLSSALRNSRPVSPQ-EPYLHQDVPELLDWRKKGAVTAVKDQGSCGACW 142
           +T+ E+++  LG     +  R  S + E  +  ++PE +DWRKKGAV  VKDQG CG+CW
Sbjct: 104 LTNDEYRSKYLGAKMEKKGERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCW 163

Query: 143 SFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHGIDTED 202
           +FS  GA+EGINQI TG LI++SEQEL+DCD SYN GC GGLMDYA++F+IKN GIDT+ 
Sbjct: 164 AFSTIGAVEGINQIVTGDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDK 223

Query: 203 DYPFQGRDGSC----RKDKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQLY 262
           DYP++G DG+C    +  KVVTID Y DVP  +EE L +AVA QP+S+ I    RAFQLY
Sbjct: 224 DYPYKGVDGTCDQIRKNAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLY 283

Query: 263 SKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGVC 322
             GIF G C T LDH V+ VGYG+ENG DYWIV+NSWGK WG  GY+ M RN  +S G C
Sbjct: 284 DSGIFDGSCGTQLDHGVVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKC 343

Query: 323 GINMLASYPIKTSPNP------PPSPPPGPTKCSFLTSCAAGETCCCAKEFFGLCLSWKC 382
           GI +  SYPIK   NP      PPSP   PT+C    +C    TCCC  E+   C +W C
Sbjct: 344 GIAIEPSYPIKNGENPPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGC 403

Query: 383 CGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENR--SPSGTSGR 424
           C L +A CC D   CCP +YP+CD  +  CL    +    +AL+ +  +P  + GR
Sbjct: 404 CPLEAATCCDDNYSCCPHEYPVCDLDQGTCLLSKNSPFSVKALKRKPATPFWSQGR 458

BLAST of CmaCh12G004380 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 415.2 bits (1066), Expect = 6.2e-116
Identity = 205/410 (50.00%), Postives = 256/410 (62.44%), Query Frame = 0

Query: 23  SDISELFEIWCTEHGKSYSS----AEEKLYRLGVFADNYEFVTHHNNQGNSSYTLSLNAY 82
           S++  ++E W  EHGK   +      EK  R  +F DN  F+  HN + N SY L L  +
Sbjct: 44  SEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTK-NLSYKLGLTRF 103

Query: 83  ADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDVPELLDWRKKGAVTAVKDQGSCGAC 142
           AD+T+ E+++  LG     R  +     +  +   +P+ +DWRK+GAV  VKDQGSCG+C
Sbjct: 104 ADLTNEEYRSMYLGAKPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSC 163

Query: 143 WSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHGIDTE 202
           W+FS  GA+EGIN+I TG LIS+SEQEL+DCD SYN GC GGLMDYA++F+IKN GIDTE
Sbjct: 164 WAFSTIGAVEGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTE 223

Query: 203 DDYPFQGRDGSC----RKDKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQL 262
            DYP++  DG C    +  KVVTID Y DVP N+E  L +A+A QP+SV I    RAFQL
Sbjct: 224 ADYPYKAADGRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQL 283

Query: 263 YSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGV 322
           YS G+F G C T LDH V+ VGYG+ENG DYWIV+NSWG RWG  GYI M RN     G 
Sbjct: 284 YSSGVFDGLCGTELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGK 343

Query: 323 CGINMLASYPIKTSPNP------PPSPPPGPTKCSFLTSCAAGETCCCAKEFFGLCLSWK 382
           CGI M ASYPIK   NP      PPSP   PT C    SC    TCCC  ++   C  W 
Sbjct: 344 CGIAMEASYPIKKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWG 403

Query: 383 CCGLSSAVCCKDGRHCCPFDYPICDPQRNLCLKRTMNGTRTEALENRSPS 419
           CC L +A CC D   CCP +YP+CD  R  CL    +    +AL+ R+P+
Sbjct: 404 CCPLEAATCCDDNSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALK-RTPA 451

BLAST of CmaCh12G004380 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 353.6 bits (906), Expect = 2.2e-97
Identity = 172/317 (54.26%), Postives = 218/317 (68.77%), Query Frame = 0

Query: 18  HFSATSDISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHNNQGNSSYTLSLNA 77
           H + T  + ELFE W +EH K+Y S EEK++R  VF +N   +   NN+ N SY L LN 
Sbjct: 40  HLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEIN-SYWLGLNE 99

Query: 78  YADITHHEFKAARLGLSSALRNSRPVSPQEPYLHQDV---PELLDWRKKGAVTAVKDQGS 137
           +AD+TH EFK   LGL+   + SR   P   + ++D+   P+ +DWRKKGAV  VKDQG 
Sbjct: 100 FADLTHEEFKGRYLGLAKP-QFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQ 159

Query: 138 CGACWSFSATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHG 197
           CG+CW+FS   A+EGINQI TG+L S+SEQELIDCD ++NSGC GGLMDYA+Q++I   G
Sbjct: 160 CGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCDTTFNSGCNGGLMDYAFQYIISTGG 219

Query: 198 IDTEDDYPFQGRDGSCRKDKV----VTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSER 257
           +  EDDYP+   +G C++ K     VTI GY DVP N++E L++A+A QPVSV I  S R
Sbjct: 220 LHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGR 279

Query: 258 AFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGN 317
            FQ Y  G+F+G C T LDH V  VGYGS  G DY IVKNSWG RWG  G+I M+RN+G 
Sbjct: 280 DFQFYKGGVFNGKCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGK 339

Query: 318 SEGVCGINMLASYPIKT 328
            EG+CGIN +ASYP KT
Sbjct: 340 PEGLCGINKMASYPTKT 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT781.4e-11750.61Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S... [more]
P257765.5e-11752.24Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=... [more]
P432973.5e-11649.76Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1[more]
Q9FMH88.7e-11550.00Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S... [more]
P257772.8e-10549.37Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1KL322.7e-25299.06zingipain-2 OS=Cucurbita maxima OX=3661 GN=LOC111496216 PE=3 SV=1[more]
A0A6J1GH926.9e-24897.42zingipain-2 OS=Cucurbita moschata OX=3662 GN=LOC111454188 PE=3 SV=1[more]
A0A1S3BBY81.3e-22287.32zingipain-2 OS=Cucumis melo OX=3656 GN=LOC103488009 PE=3 SV=1[more]
A0A5A7VC451.2e-22087.09Zingipain-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005350 PE... [more]
A0A0A0LNP72.1e-22086.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G362510 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023002351.15.6e-25299.06zingipain-2 [Cucurbita maxima][more]
XP_022951321.11.4e-24797.42zingipain-2 [Cucurbita moschata][more]
XP_023538152.13.2e-24797.42zingipain-2 [Cucurbita pepo subsp. pepo][more]
KAG6585568.13.5e-24696.96putative cysteine protease RD21C, partial [Cucurbita argyrosperma subsp. sororia... [more]
KAG7020481.12.7e-23092.42putative cysteine protease RD21C [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G09850.16.5e-17469.30xylem bark cysteine peptidase 3 [more]
AT3G19390.11.0e-11850.61Granulin repeat cysteine protease family protein [more]
AT1G47128.12.5e-11749.76Granulin repeat cysteine protease family protein [more]
AT5G43060.16.2e-11650.00Granulin repeat cysteine protease family protein [more]
AT4G35350.12.2e-9754.26xylem cysteine peptidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 132..147
score: 58.83
coord: 270..280
score: 62.56
coord: 285..291
score: 79.08
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 114..325
e-value: 3.7E-119
score: 411.8
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 114..325
e-value: 3.7E-78
score: 262.6
IPR000118GranulinSMARTSM00277GRAN_2coord: 342..399
e-value: 8.1E-21
score: 85.2
IPR000118GranulinPFAMPF00396Granulincoord: 353..399
e-value: 3.7E-10
score: 40.0
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 29..86
e-value: 4.3E-23
score: 92.7
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 29..86
e-value: 6.2E-16
score: 58.6
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 13..327
e-value: 4.2E-112
score: 376.7
NoneNo IPR availablePANTHERPTHR12411:SF414OS05G0508300 PROTEINcoord: 15..358
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 15..358
NoneNo IPR availableSUPERFAMILY57277Granulin repeatcoord: 339..372
IPR037277Granulin superfamilyGENE3D2.10.25.160Granulincoord: 340..410
e-value: 4.5E-10
score: 41.6
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 268..278
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 132..143
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 285..304
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 115..324
e-value: 1.0278E-107
score: 314.562
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 25..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G004380.1CmaCh12G004380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity