CmaCh12G000470 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh12G000470 vs. ExPASy Swiss-Prot
Match: A0A2G3D6U1 (Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 6.3e-63
Identity = 158/443 (35.67%), Postives = 239/443 (53.95%), Query Frame = 0

Query: 5   IVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAA------SPNPPCTS 64
           +  +  IKPSS TP  LR H LS  DQ   +MY+P  FF+ +          S      +
Sbjct: 12  VCDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  HFLQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVE 124
           H LQ SLS TL  YYP+AG++KD  TVDCND G  FL  R++   +++EIL+ P + + E
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIK--CSMSEILDHPHASLAE 131

Query: 125 MLFV-DGLQWKHS-KMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTR 184
            + +   L W ++ + G LL +QV+ F CGG A++V  SHK+GD  +L+ F+ DW  +TR
Sbjct: 132 SIVLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVTR 191

Query: 185 DTSTSAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKN-FTCKRFVFEASKIEALKRSIS 244
           D +T+     +P F    +F       ++   I    N    KR +F   K++AL+  ++
Sbjct: 192 DHTTTTL-VPSPRFVGDSVFSTKTYGSLTTPQILSDLNECVQKRLIFPTDKLDALRAKVA 251

Query: 245 DK--VKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMG 304
           ++  VKNPTR EVV+AL++     A      + +  P+ L+  +N+RT + P LP +T+G
Sbjct: 252 EESGVKNPTRAEVVSALLFKCATKA------SSSMLPSKLVHFLNIRTMIKPRLPRNTIG 311

Query: 305 NIVSFFPVAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVRE 364
           N+ S F +   A   +++E+  +V  +RKE+     + A K    E  ELI + +  +RE
Sbjct: 312 NLSSIFSI--EATNMQDMELPTLVRNLRKEV-----EVAYKKDQVEQNELILEVVESMRE 371

Query: 365 ---ILSKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGI 424
                      + VY CS+ CK+P Y VDFGWG    V +     KN   + D K G G+
Sbjct: 372 GKLPFENMDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGV 431

Query: 425 EALVCLDDKEMGGFQHNEELLAY 434
           EA V L  ++M  F+ NEELL +
Sbjct: 432 EARVMLHKQQMSEFERNEELLEF 438

BLAST of CmaCh12G000470 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.1e-62
Identity = 158/446 (35.43%), Postives = 243/446 (54.48%), Query Frame = 0

Query: 5   IVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAA------SPNPPCTS 64
           + ++  IKPSS TP  LR H LS  DQ   +MY+P  FF+ +          S      +
Sbjct: 12  VCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  HFLQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVE 124
           H LQ SLS TL  YYP+AG++KD  TVDCND G  FL  R++   +++EIL+ P + + E
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIK--CSMSEILDHPHASLAE 131

Query: 125 MLFV-DGLQWKHS-KMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTR 184
            + +   L W ++ + G LL +QV+ F CGG A++V  SHK+GD  +L+ F+ DW  +TR
Sbjct: 132 SIVLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVTR 191

Query: 185 DTSTSAQHTVNPLFHSADLF---PLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRS 244
           D +T+     +P F    +F     G L  ++   + +      KR +F   K++AL+  
Sbjct: 192 DRTTTTL-VPSPRFVGDSVFSTQKYGSL--ITPQILSDLNQCVQKRLIFPTDKLDALRAK 251

Query: 245 ISDK--VKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPEST 304
           ++++  VKNPTR EVV+AL++     A      + +  P+ L+  +N+RT + P LP + 
Sbjct: 252 VAEESGVKNPTRAEVVSALLFKCATKA------SSSMLPSKLVHFLNIRTMIKPRLPRNA 311

Query: 305 MGNIVSFFPVAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEV 364
           +GN+ S F +   A   +++E+  +V  +RKE+     + A K    E  ELI + +  +
Sbjct: 312 IGNLSSIFSI--EATNMQDMELPTLVRNLRKEV-----EVAYKKDQVEQNELILEVVESM 371

Query: 365 RE----ILSKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNG 424
           RE      + +G    VY CS+ CK+P Y VDFGWG    V +     KN   + D K G
Sbjct: 372 REGKLPFENMDGYKN-VYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAG 431

Query: 425 GGIEALVCLDDKEMGGFQHNEELLAY 434
            G+EA V L  ++M  F+ NEELL +
Sbjct: 432 QGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of CmaCh12G000470 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.1e-62
Identity = 158/445 (35.51%), Postives = 240/445 (53.93%), Query Frame = 0

Query: 5   IVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAA------SPNPPCTS 64
           I  +  IKPSS TP  LR H LS  DQ   +MY+P  FF+ +          S      +
Sbjct: 12  ICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  HFLQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVE 124
           H LQ SLS TL  YYP+AG++KD  TVDCND G  FL  R++   +++EIL+ P + + E
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIK--CSMSEILDHPHASLAE 131

Query: 125 MLFV-DGLQWKHS-KMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTR 184
            + +   L W ++ + G LL +QV+ F CGG A++V  SHK+GD  +L+ F+ DW  +TR
Sbjct: 132 SIVLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVTR 191

Query: 185 DTSTSAQHTVNPLFHSADLF---PLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRS 244
           D +T+     +P F    +F     G L  ++   + +      KR +F   K++AL+  
Sbjct: 192 DHTTTTL-VPSPRFVGDSVFSTKKYGSL--ITPQILSDLNECVQKRLIFPTDKLDALRAK 251

Query: 245 ISDK--VKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPEST 304
           ++++  VKNPTR EVV+AL++     A      + +  P+ L+  +N+RT + P LP + 
Sbjct: 252 VAEESGVKNPTRAEVVSALLFKCATKA------SSSMLPSKLVHFLNIRTMIKPRLPRNA 311

Query: 305 MGNIVSFFPVAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEV 364
           +GN+ S F +   A   +++E+  +V  +RKE+     + A K    E  ELI + +  +
Sbjct: 312 IGNLSSIFSI--EATNMQDMELPTLVRNLRKEV-----EVAYKKDQVEQNELILEVVESM 371

Query: 365 RE---ILSKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGG 424
           RE           + VY CS+ CK+P Y VDFGWG    V +     KN   + D K G 
Sbjct: 372 REGKLPFENMDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQ 431

Query: 425 GIEALVCLDDKEMGGFQHNEELLAY 434
           G+EA V L  ++M  F+ NEELL +
Sbjct: 432 GVEARVMLHKQQMSEFERNEELLEF 438

BLAST of CmaCh12G000470 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.8e-55
Identity = 147/436 (33.72%), Postives = 226/436 (51.83%), Query Frame = 0

Query: 4   QIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHFLQR 63
           +IVS+  IKPSSPTP  LR + LS  DQ        L FF+   +  S         L+ 
Sbjct: 7   EIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFY--TYNPSHESSKIGDLLKN 66

Query: 64  SLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEMLFVD 123
           SLS TL  YY FAGR+ +   +DCNDEGV F+E R+ G   + +IL+  KS   +++   
Sbjct: 67  SLSKTLVSYYQFAGRLIENDYIDCNDEGVEFVEVRIHG--RMNDILKRGKSFATDLVLPT 126

Query: 124 GLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTSTSAQ 183
            +   H     LL +Q+++F CGG A+    SHK+ D  + V F+ DW      TS S  
Sbjct: 127 RIIALHE--DSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDW---ASSTSLSTF 186

Query: 184 HTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALK-RSISDKVKNPT 243
           H   PL  +  +FP  D   +S  +I   +    KRFVF    IE LK ++I   ++ P+
Sbjct: 187 HKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEYGIQKPS 246

Query: 244 RVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFPVA 303
           RVEVVTA  ++   +A  D    P   P  ++  +NLR  +   LP++++GNI SF+   
Sbjct: 247 RVEVVTA--FLCQCAANCDL---PRKKPYAIISAVNLRPYLA--LPQNSIGNIFSFYFCI 306

Query: 304 AAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGKSQ 363
                + +     ++  +R    +  +    K +        + ++ E+ + L +   S 
Sbjct: 307 NDEGMDNQFSA--LISKLRNGKQKLLENIISKEKL-----TYESQMQELTKCLDQLNISS 366

Query: 364 L-VYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKEM 423
           L  Y CSS C+FPVY +DFGWG    V+     +K+ +++MD   G GIEAL+ +++K+M
Sbjct: 367 LDTYFCSSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALITMEEKKM 419

Query: 424 GGFQHNEELLAYASLN 438
             F+ NEEL ++A L+
Sbjct: 427 AAFEKNEELRSFAYLD 419

BLAST of CmaCh12G000470 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 5.3e-54
Identity = 140/430 (32.56%), Postives = 219/430 (50.93%), Query Frame = 0

Query: 2   QPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHFL 61
           Q QI+S + I+PSSPTP  L+TH LS  DQ+    ++P++ F+     ++ +    S  L
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRSENL 63

Query: 62  QRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVT--LAEILEGPKSEIVEM 121
           +RSLS  L+++YP AGRI    +VDCND GV FLEAR+   ++  +  +     ++ +  
Sbjct: 64  KRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYLPF 123

Query: 122 LFVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTS 181
               G +    K    LA++++ F+CGG A+ V +SHK+ D  +L  F+  W      T+
Sbjct: 124 QPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSW------TA 183

Query: 182 TSAQHT--VNPLFHSADLFPLGDLPPMSGAAIEE---GKNFTCKRFVFEASKIEALKRSI 241
           T  + T  V P F           PPM      E    +N   KRFVF+  K+EALK  +
Sbjct: 184 TCQEETDIVQPNFDLGS----HHFPPMESIPAPEFLPDENIVMKRFVFDKEKLEALKAQL 243

Query: 242 SD---KVKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPEST 301
           +    +VKN +RV++V A+I+   I   R      N    +  Q +NLR+RM PP P+S 
Sbjct: 244 ASSATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNK--LVAAQAVNLRSRMNPPFPQSA 303

Query: 302 MGNIVSFFPVAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEV 361
           MGNI +     A A  E + +  ++VG ++  + +  D++ K+             L + 
Sbjct: 304 MGNIATM----AYAVAEEDKDFSDLVGPLKTSLAKIDDEHVKE-------------LQKG 363

Query: 362 REILSKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIE 421
              L    + Q ++  SS C+   Y +DFGWG    V    + MKN V +MD +N  G+E
Sbjct: 364 VTYLDYEAEPQELFSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGME 404

BLAST of CmaCh12G000470 vs. ExPASy TrEMBL
Match: A0A6J1KJX5 (acylsugar acyltransferase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111496403 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 3.7e-260
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF
Sbjct: 16  MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 75

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML
Sbjct: 76  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 135

Query: 121 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 180
           FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST
Sbjct: 136 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 195

Query: 181 SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN 240
           SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN
Sbjct: 196 SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN 255

Query: 241 PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 300
           PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP
Sbjct: 256 PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 315

Query: 301 VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK 360
           VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK
Sbjct: 316 VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK 375

Query: 361 SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE 420
           SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE
Sbjct: 376 SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE 435

Query: 421 MGGFQHNEELLAYASLNPTANCANYANY 449
           MGGFQHNEELLAYASLNPTANCANYANY
Sbjct: 436 MGGFQHNEELLAYASLNPTANCANYANY 463

BLAST of CmaCh12G000470 vs. ExPASy TrEMBL
Match: A0A6J1GI35 (acylsugar acyltransferase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111454400 PE=3 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.6e-240
Identity = 416/445 (93.48%), Postives = 425/445 (95.51%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           MQ QIVSRQSIKPSSPTPPHL+THALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF
Sbjct: 16  MQLQIVSRQSIKPSSPTPPHLKTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 75

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEA L G VTLAEILEGPKSEIVEML
Sbjct: 76  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEASLGGNVTLAEILEGPKSEIVEML 135

Query: 121 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 180
           FVDGLQWKHSKMGPLLAIQVTYFQCGGFAV VLLSHKLGDLATLVKFVTDWGF+TRDT+ 
Sbjct: 136 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVGVLLSHKLGDLATLVKFVTDWGFITRDTTN 195

Query: 181 SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN 240
           SAQHTVNPLFHSADLFPLGDLPPMSGA IEEGKN TCKRFVFEASKIEALKRSISDKVK 
Sbjct: 196 SAQHTVNPLFHSADLFPLGDLPPMSGAVIEEGKNLTCKRFVFEASKIEALKRSISDKVKI 255

Query: 241 PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 300
           PTRVEV+TALIY AIISALRDSN NPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP
Sbjct: 256 PTRVEVMTALIYKAIISALRDSNSNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 315

Query: 301 VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK 360
           V AAAAGEREVEVHEVVGVMRKEMGEFCDKYAKK+RTEEWVELIKKRLNEVREILSKNGK
Sbjct: 316 V-AAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKFRTEEWVELIKKRLNEVREILSKNGK 375

Query: 361 SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE 420
            QL+YRCSSGC FPVYGVDFGWG AAWVTMAA +MKNTVMM+DDKNGGGIEAL+ LDDKE
Sbjct: 376 PQLIYRCSSGCNFPVYGVDFGWGAAAWVTMAAFKMKNTVMMLDDKNGGGIEALISLDDKE 435

Query: 421 MGGFQHNEELLAYASLNPTANCANY 446
           M  FQHNEELLAY SLNPTANCANY
Sbjct: 436 MAAFQHNEELLAYTSLNPTANCANY 459

BLAST of CmaCh12G000470 vs. ExPASy TrEMBL
Match: A0A1S3BB28 (vinorine synthase OS=Cucumis melo OX=3656 GN=LOC103487747 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 7.2e-171
Identity = 314/446 (70.40%), Postives = 361/446 (80.94%), Query Frame = 0

Query: 4   QIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSH---- 63
           QIVS Q IKPSSPTPP+L  H LSLFDQLAPH +VPLVFFF   H          H    
Sbjct: 10  QIVSTQIIKPSSPTPPNLNIHTLSLFDQLAPHTFVPLVFFF--SHQGPGRSGTYYHTTVD 69

Query: 64  ---FLQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEI 123
               L++SLS TLSRYYPFAGRIKDK+++DCNDEGVTF+EA LEG VT+++ILE P+SEI
Sbjct: 70  KLELLRQSLSTTLSRYYPFAGRIKDKVSIDCNDEGVTFVEAWLEG-VTVSKILENPRSEI 129

Query: 124 VEMLFVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTR 183
           VEMLFVDGLQWK SKMG LL +Q+T F+CGG ++ VLLSHKLGDLATLVKFV DWG +TR
Sbjct: 130 VEMLFVDGLQWKDSKMGSLLKVQITLFECGGLSIGVLLSHKLGDLATLVKFVQDWGLITR 189

Query: 184 DTSTSAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISD 243
           +     + TVNPLF SAD+FP GDLP MSGA IEEG NFTCKRFVFE SKIE+LK  IS+
Sbjct: 190 NNGFCEEETVNPLFFSADVFPHGDLPAMSGAVIEEG-NFTCKRFVFEGSKIESLKNRISE 249

Query: 244 KVKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIV 303
           KV+NP+RVEVV+ALIY AIISA R++N N  N  TLLLQT+NLR R+VPPLPE+ +G++V
Sbjct: 250 KVQNPSRVEVVSALIYKAIISASRNNN-NSQNQSTLLLQTLNLRKRVVPPLPETLVGSLV 309

Query: 304 SFFPVAAAAAGERE-VEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREIL 363
           SFFPV     GERE VE+HE+VG MRKEMGEFCD+YAKKYRT+EW+ELIKKRLNE REIL
Sbjct: 310 SFFPV--GVDGEREVVELHELVGTMRKEMGEFCDRYAKKYRTKEWLELIKKRLNESREIL 369

Query: 364 SKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVC 423
           SKNG +QLVYRCSSGC FP+Y VDFGWG A W+TM A +MKNTVMM+D KNGGGIEAL+ 
Sbjct: 370 SKNGNNQLVYRCSSGCNFPIYEVDFGWGVADWITMPAFKMKNTVMMLDAKNGGGIEALIS 429

Query: 424 LDDKEMGGFQHNEELLAYASLNPTAN 442
           L D EM  FQ NEELLA+ASLNP+ N
Sbjct: 430 LQDGEMAAFQDNEELLAFASLNPSVN 448

BLAST of CmaCh12G000470 vs. ExPASy TrEMBL
Match: A0A0A0LNF8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350350 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 7.4e-168
Identity = 305/442 (69.00%), Postives = 358/442 (81.00%), Query Frame = 0

Query: 4   QIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRR--PHAASPNPPCTSHFL 63
           QIVS Q IKPSSPTPP+L  H LSLFDQLAPH +VPL+FFF    P + +     T   L
Sbjct: 10  QIVSTQIIKPSSPTPPNLNIHTLSLFDQLAPHTFVPLLFFFSHHGPSSGTYYDHTTVELL 69

Query: 64  QRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEMLF 123
           ++SLS TLSRYYP AGRIKDK+++DCNDEGVTF+EARLEG VT+++ILE P+SEIVE LF
Sbjct: 70  RQSLSTTLSRYYPLAGRIKDKVSIDCNDEGVTFVEARLEG-VTVSKILENPRSEIVEKLF 129

Query: 124 VDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTS-T 183
           VDGLQWK SKMG LL +Q+T F+CGG ++ VLLSHKLGDLATLVKF+ DWG +TR+    
Sbjct: 130 VDGLQWKDSKMGSLLKVQITLFECGGLSIGVLLSHKLGDLATLVKFIQDWGVITRNNGFC 189

Query: 184 SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN 243
             +  +NPLF S DLFP GDLP MSGA IEEG NFTCKRFVFE SKIE+LK  IS+KV+N
Sbjct: 190 DEEEIINPLFFSGDLFPHGDLPAMSGAVIEEG-NFTCKRFVFEGSKIESLKNRISEKVQN 249

Query: 244 PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 303
           P+RVEVV+ALIY AIISA R +N + N+  TLLLQT+NLR R+ PPLP++ +G++VSFFP
Sbjct: 250 PSRVEVVSALIYKAIISASRTNNNSQNHPATLLLQTLNLRKRVAPPLPDTLVGSLVSFFP 309

Query: 304 VAAAAAGERE-VEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNG 363
           V     GERE VE+HE+VG MRKEMGEFC+KYAKKYRT+EW+ELIKKRLNE REILSKNG
Sbjct: 310 V--GVDGEREVVELHELVGTMRKEMGEFCEKYAKKYRTKEWLELIKKRLNESREILSKNG 369

Query: 364 KSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDK 423
            +QLVYRCSSGC FP+Y VDFGWG A W+TM A +MKNTVMM+D KNG GIEALV L D 
Sbjct: 370 NNQLVYRCSSGCNFPIYEVDFGWGAADWITMPAFKMKNTVMMLDAKNGRGIEALVSLQDG 429

Query: 424 EMGGFQHNEELLAYASLNPTAN 442
           EM  FQ NEELLA+ASLNP+ +
Sbjct: 430 EMAAFQDNEELLAFASLNPSVH 447

BLAST of CmaCh12G000470 vs. ExPASy TrEMBL
Match: A0A5A7V5H2 (Vinorine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001670 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 4.8e-167
Identity = 311/446 (69.73%), Postives = 358/446 (80.27%), Query Frame = 0

Query: 4   QIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSH---- 63
           QIVS Q IKPSSPTPP+L  H LSLFDQLAPH +VPLVFFF   H          H    
Sbjct: 10  QIVSTQIIKPSSPTPPNLNIHTLSLFDQLAPHTFVPLVFFF--SHQGPGRSGTYYHTTVD 69

Query: 64  ---FLQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEI 123
               L++SLS TLSRYYPFAGRIKDK+++DCNDEGVTF+EA LEG VT+++ILE P+SEI
Sbjct: 70  KLELLRQSLSTTLSRYYPFAGRIKDKVSIDCNDEGVTFVEAWLEG-VTVSKILENPRSEI 129

Query: 124 VEMLFVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTR 183
           VEMLFVDGLQWK SKMG LL +Q+T F+CGG ++ VLLSHKLGDLATLVKFV DWG +TR
Sbjct: 130 VEMLFVDGLQWKDSKMGSLLKVQITLFECGGLSIGVLLSHKLGDLATLVKFVQDWGLITR 189

Query: 184 DTSTSAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISD 243
           +     + TVNPLF SAD+FP GDLP MSGA IEEG NFT   FVFE SKIE+LK  IS+
Sbjct: 190 NNGFCEEETVNPLFFSADVFPHGDLPAMSGAVIEEG-NFT---FVFEGSKIESLKNRISE 249

Query: 244 KVKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIV 303
           KV+NP+RVEVV+ALIY AIISA R++N N  N  TLLLQT+NLR R+VPPLPE+ +G++V
Sbjct: 250 KVQNPSRVEVVSALIYKAIISASRNNN-NSQNQSTLLLQTLNLRKRVVPPLPETLVGSLV 309

Query: 304 SFFPVAAAAAGERE-VEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREIL 363
           SFFPV     GERE VE+HE+VG MRKEMGEFCD+YAKKYRT+EW+ELIKKRLNE REIL
Sbjct: 310 SFFPV--GVDGEREVVELHELVGTMRKEMGEFCDRYAKKYRTKEWLELIKKRLNESREIL 369

Query: 364 SKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVC 423
           SKNG +QLVYRCSSGC FP+Y VDFGWG A W+TM A +MKNTVMM+D KNGGGIEAL+ 
Sbjct: 370 SKNGNNQLVYRCSSGCNFPIYEVDFGWGVADWITMPAFKMKNTVMMLDAKNGGGIEALIS 429

Query: 424 LDDKEMGGFQHNEELLAYASLNPTAN 442
           L D EM  FQ NEELLA+ASLNP+ N
Sbjct: 430 LQDGEMAAFQDNEELLAFASLNPSVN 445

BLAST of CmaCh12G000470 vs. NCBI nr
Match: XP_023002587.1 (acylsugar acyltransferase 3-like [Cucurbita maxima])

HSP 1 Score: 906.7 bits (2342), Expect = 7.7e-260
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF
Sbjct: 16  MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 75

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML
Sbjct: 76  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 135

Query: 121 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 180
           FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST
Sbjct: 136 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 195

Query: 181 SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN 240
           SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN
Sbjct: 196 SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN 255

Query: 241 PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 300
           PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP
Sbjct: 256 PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 315

Query: 301 VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK 360
           VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK
Sbjct: 316 VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK 375

Query: 361 SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE 420
           SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE
Sbjct: 376 SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE 435

Query: 421 MGGFQHNEELLAYASLNPTANCANYANY 449
           MGGFQHNEELLAYASLNPTANCANYANY
Sbjct: 436 MGGFQHNEELLAYASLNPTANCANYANY 463

BLAST of CmaCh12G000470 vs. NCBI nr
Match: XP_023538329.1 (acylsugar acyltransferase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 851.7 bits (2199), Expect = 2.9e-243
Identity = 421/448 (93.97%), Postives = 429/448 (95.76%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           MQ QIVSRQSIKPSSPTPPHL+THALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF
Sbjct: 16  MQLQIVSRQSIKPSSPTPPHLKTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 75

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARL G VTLAEILEGPKSEIVEML
Sbjct: 76  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLGGNVTLAEILEGPKSEIVEML 135

Query: 121 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 180
           FVDGLQWKHSKMGPLLAIQVTYFQCGGFAV VLLSHKLGDLATLVKFVTDWGF+TRDT+T
Sbjct: 136 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVGVLLSHKLGDLATLVKFVTDWGFITRDTTT 195

Query: 181 SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN 240
           SAQHTVNPLFHSADLFPLGDLPPMS A IEEGKN TCKRFVFEASKIEALKRSISDKVKN
Sbjct: 196 SAQHTVNPLFHSADLFPLGDLPPMSSAVIEEGKNLTCKRFVFEASKIEALKRSISDKVKN 255

Query: 241 PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 300
           PTRVEVVTALIY AIISALRDSN NPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP
Sbjct: 256 PTRVEVVTALIYKAIISALRDSNSNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 315

Query: 301 VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK 360
           VAAAA+GEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRL EVREILSKNGK
Sbjct: 316 VAAAASGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLKEVREILSKNGK 375

Query: 361 SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE 420
            QL+YRCSSGC FPVYGVDFGWG AAWVTMAA +MKNTVMM+DDKNGGGIEAL+ LDDKE
Sbjct: 376 PQLIYRCSSGCNFPVYGVDFGWGAAAWVTMAAFKMKNTVMMLDDKNGGGIEALLSLDDKE 435

Query: 421 MGGFQHNEELLAYASLNPTANCANYANY 449
           M  FQHNEELLAYASLNPTAN ANY NY
Sbjct: 436 MAAFQHNEELLAYASLNPTANYANYGNY 463

BLAST of CmaCh12G000470 vs. NCBI nr
Match: KAG6585194.1 (Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020112.1 Acylsugar acyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 840.9 bits (2171), Expect = 5.2e-240
Identity = 417/445 (93.71%), Postives = 425/445 (95.51%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           MQ QIVSRQSIKPSSPTPPHL+THALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF
Sbjct: 1   MQLQIVSRQSIKPSSPTPPHLKTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEA L G VTLAEILEGPKSEIVEML
Sbjct: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEASLGGNVTLAEILEGPKSEIVEML 120

Query: 121 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 180
           FVDGLQWKHSKMGPLLAIQVTYFQCGGFAV VLLSHKLGDLATLVKFVTDWGF+TRDT+ 
Sbjct: 121 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVGVLLSHKLGDLATLVKFVTDWGFITRDTTN 180

Query: 181 SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN 240
           SAQHTVNPLFHSADLFPLGDLPPMSGA IEEGKN TCKRFVFEASKIEALKRSISDKVK 
Sbjct: 181 SAQHTVNPLFHSADLFPLGDLPPMSGAVIEEGKNLTCKRFVFEASKIEALKRSISDKVKI 240

Query: 241 PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 300
           PTRVEVVTALIY AIISALRDSN NPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP
Sbjct: 241 PTRVEVVTALIYKAIISALRDSNSNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 300

Query: 301 VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK 360
           V AAAAGEREVEVHEVVGVMRKEMGEFCDKYAKK+RTEEWVELIKKRLNEVREILSKNGK
Sbjct: 301 V-AAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKFRTEEWVELIKKRLNEVREILSKNGK 360

Query: 361 SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE 420
            QL+YRCSSGC FPVYGVDFGWG AAWVTMAA +MKNTVMM+DDKNGGGIEAL+ LDDKE
Sbjct: 361 PQLIYRCSSGCNFPVYGVDFGWGAAAWVTMAAFKMKNTVMMLDDKNGGGIEALISLDDKE 420

Query: 421 MGGFQHNEELLAYASLNPTANCANY 446
           M  FQHNEELLAY SLNPTANCANY
Sbjct: 421 MAAFQHNEELLAYTSLNPTANCANY 444

BLAST of CmaCh12G000470 vs. NCBI nr
Match: XP_022951646.1 (acylsugar acyltransferase 3-like [Cucurbita moschata])

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-239
Identity = 416/445 (93.48%), Postives = 425/445 (95.51%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           MQ QIVSRQSIKPSSPTPPHL+THALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF
Sbjct: 16  MQLQIVSRQSIKPSSPTPPHLKTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 75

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEA L G VTLAEILEGPKSEIVEML
Sbjct: 76  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEASLGGNVTLAEILEGPKSEIVEML 135

Query: 121 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 180
           FVDGLQWKHSKMGPLLAIQVTYFQCGGFAV VLLSHKLGDLATLVKFVTDWGF+TRDT+ 
Sbjct: 136 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVGVLLSHKLGDLATLVKFVTDWGFITRDTTN 195

Query: 181 SAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISDKVKN 240
           SAQHTVNPLFHSADLFPLGDLPPMSGA IEEGKN TCKRFVFEASKIEALKRSISDKVK 
Sbjct: 196 SAQHTVNPLFHSADLFPLGDLPPMSGAVIEEGKNLTCKRFVFEASKIEALKRSISDKVKI 255

Query: 241 PTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 300
           PTRVEV+TALIY AIISALRDSN NPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP
Sbjct: 256 PTRVEVMTALIYKAIISALRDSNSNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIVSFFP 315

Query: 301 VAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREILSKNGK 360
           V AAAAGEREVEVHEVVGVMRKEMGEFCDKYAKK+RTEEWVELIKKRLNEVREILSKNGK
Sbjct: 316 V-AAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKFRTEEWVELIKKRLNEVREILSKNGK 375

Query: 361 SQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVCLDDKE 420
            QL+YRCSSGC FPVYGVDFGWG AAWVTMAA +MKNTVMM+DDKNGGGIEAL+ LDDKE
Sbjct: 376 PQLIYRCSSGCNFPVYGVDFGWGAAAWVTMAAFKMKNTVMMLDDKNGGGIEALISLDDKE 435

Query: 421 MGGFQHNEELLAYASLNPTANCANY 446
           M  FQHNEELLAY SLNPTANCANY
Sbjct: 436 MAAFQHNEELLAYTSLNPTANCANY 459

BLAST of CmaCh12G000470 vs. NCBI nr
Match: XP_008444410.1 (PREDICTED: vinorine synthase [Cucumis melo])

HSP 1 Score: 610.1 bits (1572), Expect = 1.5e-170
Identity = 314/446 (70.40%), Postives = 361/446 (80.94%), Query Frame = 0

Query: 4   QIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSH---- 63
           QIVS Q IKPSSPTPP+L  H LSLFDQLAPH +VPLVFFF   H          H    
Sbjct: 10  QIVSTQIIKPSSPTPPNLNIHTLSLFDQLAPHTFVPLVFFF--SHQGPGRSGTYYHTTVD 69

Query: 64  ---FLQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEI 123
               L++SLS TLSRYYPFAGRIKDK+++DCNDEGVTF+EA LEG VT+++ILE P+SEI
Sbjct: 70  KLELLRQSLSTTLSRYYPFAGRIKDKVSIDCNDEGVTFVEAWLEG-VTVSKILENPRSEI 129

Query: 124 VEMLFVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTR 183
           VEMLFVDGLQWK SKMG LL +Q+T F+CGG ++ VLLSHKLGDLATLVKFV DWG +TR
Sbjct: 130 VEMLFVDGLQWKDSKMGSLLKVQITLFECGGLSIGVLLSHKLGDLATLVKFVQDWGLITR 189

Query: 184 DTSTSAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISD 243
           +     + TVNPLF SAD+FP GDLP MSGA IEEG NFTCKRFVFE SKIE+LK  IS+
Sbjct: 190 NNGFCEEETVNPLFFSADVFPHGDLPAMSGAVIEEG-NFTCKRFVFEGSKIESLKNRISE 249

Query: 244 KVKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGNIV 303
           KV+NP+RVEVV+ALIY AIISA R++N N  N  TLLLQT+NLR R+VPPLPE+ +G++V
Sbjct: 250 KVQNPSRVEVVSALIYKAIISASRNNN-NSQNQSTLLLQTLNLRKRVVPPLPETLVGSLV 309

Query: 304 SFFPVAAAAAGERE-VEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVREIL 363
           SFFPV     GERE VE+HE+VG MRKEMGEFCD+YAKKYRT+EW+ELIKKRLNE REIL
Sbjct: 310 SFFPV--GVDGEREVVELHELVGTMRKEMGEFCDRYAKKYRTKEWLELIKKRLNESREIL 369

Query: 364 SKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEALVC 423
           SKNG +QLVYRCSSGC FP+Y VDFGWG A W+TM A +MKNTVMM+D KNGGGIEAL+ 
Sbjct: 370 SKNGNNQLVYRCSSGCNFPIYEVDFGWGVADWITMPAFKMKNTVMMLDAKNGGGIEALIS 429

Query: 424 LDDKEMGGFQHNEELLAYASLNPTAN 442
           L D EM  FQ NEELLA+ASLNP+ N
Sbjct: 430 LQDGEMAAFQDNEELLAFASLNPSVN 448

BLAST of CmaCh12G000470 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 264.2 bits (674), Expect = 1.9e-70
Identity = 158/446 (35.43%), Postives = 257/446 (57.62%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           M+  +VSR  IKPSSPTP HL+   LSL +QL P ++ P+VFF+   ++  P        
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQL--QM 60

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           L++SLS TL+ +YP AGR+K  +++DCND G  FLEAR+     L+ +L  P S+ ++ L
Sbjct: 61  LKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNS--PLSNLLLEPSSDSLQQL 120

Query: 121 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 180
               +    ++   LLA Q ++F+CG  ++ V +SHKL D  ++  F+  W  +   +S 
Sbjct: 121 IPTSVDSIETRTRLLLA-QASFFECGSMSIGVCISHKLADATSIGLFMKSWAAI---SSR 180

Query: 181 SAQHTVN-PLFHSADLFPLGDLPPMSGAAIEEGK----NFTCKRFVFEASKIEALK-RSI 240
            +  T+  P+F +  +FP G+    S A + E +        KRF+F++S I+AL+ ++ 
Sbjct: 181 GSIKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKAS 240

Query: 241 SDKVKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGN 300
           S +V  PTRVE V+ALI+ + + A R    +  + P++L  +++LR+R+ PP  ++++GN
Sbjct: 241 SFEVNQPTRVEAVSALIWKSAMKATR--TVSGTSKPSILANSVSLRSRVSPPFTKNSIGN 300

Query: 301 IVSFFPVAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKK-YRTEEWVELIKKRLNEVRE 360
           +VS+F  A A  G  + ++  +V  +RK    F D +  K        E+I     E  +
Sbjct: 301 LVSYF-AAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGD 360

Query: 361 ILSKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGIEAL 420
           +++       ++  SS C+F +Y  DFGWG   WV   ++R KN V ++D K  GGIEA 
Sbjct: 361 MIASGDFDFYIF--SSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAW 420

Query: 421 VCLDDKEMGGFQHNEELLAYASLNPT 440
           V L+++EM  F+ + ELL +ASLNP+
Sbjct: 421 VNLNEQEMNLFEQDRELLQFASLNPS 433

BLAST of CmaCh12G000470 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 231.9 bits (590), Expect = 1.0e-60
Identity = 152/449 (33.85%), Postives = 246/449 (54.79%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           M+ + ++++ IKPSS TP  L+T  LS++D + P +Y     F+ +    SP    +SH 
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQ--SSHK 63

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           L+ SL+ TL+++YP AGRIK  +T+DCNDEG  F++AR+     L++ L  P  + ++ L
Sbjct: 64  LKTSLAETLTKFYPLAGRIKG-VTIDCNDEGAVFVDARVNN-YPLSDFLRSPDFKTLQQL 123

Query: 121 F---VDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRD 180
               V    ++ +   PLL ++ TYF CGG A+ + ++HK+ D  ++  F+  W  + R 
Sbjct: 124 LPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMAR- 183

Query: 181 TSTSAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKN---FTCKRFVFEASKIEAL--KR 240
              +     +P+F +A+ +P  +   +    ++E  N      KRFVF ASK+E L  K 
Sbjct: 184 -GEAGDIVADPVFAAANFYPPAN--ELFKFPVDENANKISQITKRFVFSASKLEELRTKA 243

Query: 241 SISDKVKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTM 300
           +  D V  P RVE VTAL++ A ++A   SN N  +   +L+Q  NLR ++   L ES +
Sbjct: 244 ASEDFVGRPKRVESVTALLWKAFVAA-ASSNTNACD-MKVLIQPSNLRPKIPSLLAESLI 303

Query: 301 GNIVSFFPVAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEVR 360
           GNI+ F  V    + + E+++ + V  +RK+ G+      K+        +  K  N + 
Sbjct: 304 GNIM-FSSVVLGISQQEEIKIEKAVRDLRKQ-GDDLQNVIKEEGGSSSAMIGSKLANLML 363

Query: 361 EILSK-NGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVT-MAALRMKNTVMMMDDKNGGGI 420
              SK + ++   Y  SS CK P+Y   FGWG   W+    A  ++N  M++D K+G GI
Sbjct: 364 TNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDGQGI 423

Query: 421 EALVCLDDKEMGGFQHNEELLAYASLNPT 440
           EA V L ++ M   + N ELLA+AS+NP+
Sbjct: 424 EAFVTLPEENMLSLEQNTELLAFASVNPS 440

BLAST of CmaCh12G000470 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-60
Identity = 154/448 (34.38%), Postives = 244/448 (54.46%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           M+ + +S++ IKPSSPTP +L+T  LS++D + P +Y     F+ +    S     TSH 
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEH--TSHK 65

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           L+ SLS TL+++YP AGRI   +TVDC DEG  F++AR+     L E L+ P  + ++ L
Sbjct: 66  LKTSLSETLTKFYPLAGRITG-VTVDCTDEGAIFVDARVNN-CPLTEFLKCPDFDALQQL 125

Query: 121 F---VDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRD 180
               V    +  +   PLL ++ TYF CGG A+ + ++HK+ D A++  F+  W    R 
Sbjct: 126 LPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARG 185

Query: 181 TSTSAQHTVNPLFHSADLFPLGDLPPMSGAAIEEGKNFT-CKRFVFEASKIEAL--KRSI 240
            + +A    +P+F  A+ +P  +      A  + GK  +  KRFVFEASK+E L  K + 
Sbjct: 186 ENDAAA-MESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAAS 245

Query: 241 SDKVKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTMGN 300
            + V  PTRVE VTALI+   +++   S     +H  +L+Q  NLR+++   L ES++GN
Sbjct: 246 EETVDQPTRVESVTALIWKCFVAS---SKTTTCDH-KVLVQLANLRSKIPSLLQESSIGN 305

Query: 301 IVSFFPVAAAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNE--VR 360
           ++ F  V  +     EV++ E V  +RK+  E       +  + +   +I  +L    + 
Sbjct: 306 LM-FSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANLMLT 365

Query: 361 EILSKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVT-MAALRMKNTVMMMDDKNGGGIE 420
                + ++   Y  SS CK P+Y   FGW    WV    +  + N  M++D K+G GIE
Sbjct: 366 NYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLIDSKDGQGIE 425

Query: 421 ALVCLDDKEMGGFQHNEELLAYASLNPT 440
           A V L ++ M  F+ N ELLA+A++NP+
Sbjct: 426 AFVTLPEENMSSFEQNPELLAFATMNPS 443

BLAST of CmaCh12G000470 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 224.2 bits (570), Expect = 2.1e-58
Identity = 160/448 (35.71%), Postives = 245/448 (54.69%), Query Frame = 0

Query: 4   QIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASP------NPPCT 63
           +I+SR+ +KPSSPTP   R   LSL D L+  MY   + F+    AA P      +   T
Sbjct: 6   EILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFY----AADPQNLLGFSTEET 65

Query: 64  SHFLQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIV 123
           S  L++SLS TL  +YP AGRI     V+CNDEG  F+EAR++ +  L+E L+ P  E +
Sbjct: 66  SLKLKKSLSKTLPIFYPLAGRIIGSF-VECNDEGAVFIEARVDHL--LSEFLKCPVPESL 125

Query: 124 EMLFVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRD 183
           E+L     + + +   P+L IQ  +F CGG  + + +SHK+ D  +L  F+  W     +
Sbjct: 126 ELLIPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWA----E 185

Query: 184 TSTSAQHTVNPLFHSADLF--PLGDLPPMSGAAIEEGKNFTC--KRFVFEASKIEALKRS 243
           +S     T+ P F ++++F  PL +LP       EE +  +C  KRFVF+ASKI+ L+  
Sbjct: 186 SSRGLGITLIPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAK 245

Query: 244 IS-DKVKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPPLPESTM 303
            S + VKNPTRVE VTAL +  +    R S+  P    ++L   +NLR + V  L E+T+
Sbjct: 246 ASRNLVKNPTRVEAVTALFWRCVTKVSRLSSLTPRT--SVLQILVNLRGK-VDSLCENTI 305

Query: 304 GNIVSFFPVA-AAAAGEREVEVHEVVGVMRKEMGEFCDKYAKKYRTEEWVELIKKRLNEV 363
           GN++S   +    AA ER  +V + +   ++     C + +K          I + L E+
Sbjct: 306 GNMLSLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKS------SSRIFELLEEI 365

Query: 364 REILSKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVT-MAALRMKNTVMMMDDKNGGGI 423
            ++  +  +  L +  +S CK  +Y  DFGWG   WVT       KN ++++D K+G GI
Sbjct: 366 GKVYGRGNEMDL-WMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGI 425

Query: 424 EALVCLDDKEMGGFQHNEELLAYASLNP 439
           EA + L +++M  F+ ++ELL  ASLNP
Sbjct: 426 EAWITLTEEQMSLFECDQELLESASLNP 432

BLAST of CmaCh12G000470 vs. TAIR 10
Match: AT5G23970.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 214.2 bits (544), Expect = 2.2e-55
Identity = 155/449 (34.52%), Postives = 234/449 (52.12%), Query Frame = 0

Query: 1   MQPQIVSRQSIKPSSPTPPHLRTHALSLFDQLAPHMYVPLVFFFRRPHAASPNPPCTSHF 60
           M+ +++S++ IKP+SP   HL+T +LSLFDQ  P  YV  +FF+   H+   +       
Sbjct: 1   MKLELLSKEVIKPASPN--HLQTLSLSLFDQFLPSTYVSAIFFY-DDHSNQED----IQR 60

Query: 61  LQRSLSATLSRYYPFAGRIKDKLTVDCNDEGVTFLEARLEGVVTLAEILEGPKSEIVEML 120
           L+ SLS TLS +YP AG+IKD +TV CNDEG  F EAR E  + L++ L  P    +   
Sbjct: 61  LKSSLSQTLSLFYPLAGQIKDGVTVHCNDEGALFTEARAE--IFLSDFLRNPSDADLIQK 120

Query: 121 FVDGLQWKHSKMGPLLAIQVTYFQCGGFAVAVLLSHKLGDLATLVKFVTDWGFLTRDTST 180
           F+        +  PLL ++V +F+  GFAVAV +SHK+ D A+L  FV  W       S 
Sbjct: 121 FIVSPDHADPETWPLLHVKVIFFKDKGFAVAVSVSHKICDAASLSTFVCSW----TKASK 180

Query: 181 SAQHTVNPLFHSADLFPLGDL----PPMSGAAIEEGKNFTCKRFVFEASKIEALKRSISD 240
               TV+P F  AD +P  D+    PP+    + E K+ T KRFVF +  IE LK   S 
Sbjct: 181 GYADTVDPEFVGADFYPPADISIEFPPL---LVHETKSKT-KRFVFGSLMIEKLKNRASS 240

Query: 241 --KVKNPTRVEVVTALIYIAIISALRDSNPNPNNHPTLLLQTMNLRTRMVPP-LPESTMG 300
             +V   TR+E +TAL+   +  A    +         + QTM+LR R+    LP   +G
Sbjct: 241 GKRVPQATRIESITALLLRCMTKAGHSKSGKVKEF--AITQTMDLRPRVSSSLLPHKAIG 300

Query: 301 NIVSFFPVAAAAAGEREVEVHEVVGVMRK---EMGEFCDKYAKKYRTEEWVELIKKRLNE 360
           N   FF      + E ++E+ E V  ++K   E+ E     ++  ++   VE  ++  + 
Sbjct: 301 NF--FFLPLLKESSESKMEIEETVSKLQKTKQELNELIRNDSEDAKSS--VEAKERIASA 360

Query: 361 VREILSKNGKSQLVYRCSSGCKFPVYGVDFGWGGAAWVTMAALRMKNTVMMMDDKNGGGI 420
           +   L +       Y  SS C+   Y  +FGWG   WV   ++  K  V++MD K+  G+
Sbjct: 361 MLSSLCEISPEMETYAVSSWCRMSFYEANFGWGKPVWVAPDSVD-KTQVVLMDSKDSEGV 420

Query: 421 EALVCLDDKEMGGFQHNEELLAYASLNPT 440
           EA V L + +M  ++H+ ELL YA+ +P+
Sbjct: 421 EARVTLPETDMAKYEHDSELLVYATPSPS 425