CmaCh11G019310 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G019310
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionclathrin coat assembly protein AP180-like
LocationCma_Chr11: 12717574 .. 12719271 (+)
RNA-Seq ExpressionCmaCh11G019310
SyntenyCmaCh11G019310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCAGCAAGTTAAGGAAGGCCATCTACGCCGTGAAAGATCAAACCAGCATCAGCCTTGCCAAAGTCTCCGCCTCCGGCAGCGGCCCCAATGCTACCTCCTTGGAAGTCACCATCCTCAAAGCCACCACTCACGACGACGTCCCTCTCGACCACCGTTACCTGGCGGAAATCCTCCTCTTCATCTCCTCCAACAAATCCCACGCCGCCGTCTGTGTTCATTCCATCGCCAAACGCATTACCAGAACCCGTAATTGGATCGTGGCCCTCAAATCTCTCAACCTCGTCTTCCGTATCTTCCAAGAAGGCGACCCGATTTTCCCCCGCGAAGTTCTCCTCGCCATGAAACGCGGCGCTAAAATCCTTAACCTCTCCAATTTCCGCGACGATTCAAATTCCAATCCGTGGGATTACACCGCTTTTGTTCGAACCTTTGCTCTGTATCTCGACGAACGCCTCGATTGCTTTCTTAACGGGAAGCTTCAAAAGCGATACACTCAAGGGAAGACTAGCCGACGGATGAACGAGCCGATTCGGGATATGAAGCCGACGATGTTAATTGATCGGATTTCTTACTGGCAGAAATTACTGGACAGAGCAATCGCCACTAGACCCACCGGATCGGCGAAGAGGAATCGATTGATTCAACATTCGCTTCACGCCGTCGTTCAAGAGAGCTTCGATTTATACAGAGATATCTCCGATGGGCTTGCGCTTCTCTTGGATAGCTTCTTCCATTTGCAGTACCAATCCTGTGTTAATGCGTTTCATGCGTGCGTGAAAGCCGCGAAGCAATTTGAGGAATTGGGTTCTTTTTATGATGTGTGTAAAAGTATTGGGGTTGGAAGAACCTCGGAATATCCGAGTGTTCAACAGCCTTCCGATGAGTTAGTCGCGACATTGCAGGAGTTTTTGAAAGATCATGCTTCCTTCCCCTGCCATGGAAATCGATCGCCGGCGGCGGCGGCGGCTCCGACGCTGATGCCGACGCCGACGAAGGTTTTTCTCTCTGGACCAACGGTGAAGTTCACCGACGAACAATCGGAATCGTCGGATAAGAATTCGGAGAGGGGGTCTGATTTTAACTCGTTTGGGGATATTAATTCCGTCGAGTATGACGGCGAGTGCTTCTCCGATTATCAGCAATCCCAGTTGGGAGATTTCAGTAATGAATCGATTCTGTCTCCGACTTTTGCGTTCTTTGAAGAAAACCAAAAGGGTTTTCAAGCGGAAGAACACCTATTTAGACTTGGGGATTGGGAGCTCGTTCTGGCGGAATCCGCTACCGCGCCGCAAAATTGGCCGGATTTCTACTCGCCGTTCGTGGGCGACAACATGTTTGTGAAGCCCTTTGCGCCGCAGCAGCAGCATCACAACAACAACCCATTTCTTCAAGATTCAAGCGATTTATTCACAGTTGCACCAACATTCAAAGCAGAGACGGAAATGACTCAAACATTTGGCGGTCATAATTTGAATGAAGCAACCACGATGGCCCCAACATTCAGAGCAGAAACAGCAACATTTCTGATTGGAGTTGATATGGGCATTGAAAACGATCCATTTGCAGTGCCGTGGGGAAGCGAATTGAGTGGAAATGATCGGAGTTTTGAAGTGATGAATCATGATAGTTTGCTGCAGCAGCAGAAATTGTGGAAGGAGCATCAAAACAAGATTATAGCGAAGCATTCGTTGTGA

mRNA sequence

ATGCCCAGCAAGTTAAGGAAGGCCATCTACGCCGTGAAAGATCAAACCAGCATCAGCCTTGCCAAAGTCTCCGCCTCCGGCAGCGGCCCCAATGCTACCTCCTTGGAAGTCACCATCCTCAAAGCCACCACTCACGACGACGTCCCTCTCGACCACCGTTACCTGGCGGAAATCCTCCTCTTCATCTCCTCCAACAAATCCCACGCCGCCGTCTGTGTTCATTCCATCGCCAAACGCATTACCAGAACCCGTAATTGGATCGTGGCCCTCAAATCTCTCAACCTCGTCTTCCGTATCTTCCAAGAAGGCGACCCGATTTTCCCCCGCGAAGTTCTCCTCGCCATGAAACGCGGCGCTAAAATCCTTAACCTCTCCAATTTCCGCGACGATTCAAATTCCAATCCGTGGGATTACACCGCTTTTGTTCGAACCTTTGCTCTGTATCTCGACGAACGCCTCGATTGCTTTCTTAACGGGAAGCTTCAAAAGCGATACACTCAAGGGAAGACTAGCCGACGGATGAACGAGCCGATTCGGGATATGAAGCCGACGATGTTAATTGATCGGATTTCTTACTGGCAGAAATTACTGGACAGAGCAATCGCCACTAGACCCACCGGATCGGCGAAGAGGAATCGATTGATTCAACATTCGCTTCACGCCGTCGTTCAAGAGAGCTTCGATTTATACAGAGATATCTCCGATGGGCTTGCGCTTCTCTTGGATAGCTTCTTCCATTTGCAGTACCAATCCTGTGTTAATGCGTTTCATGCGTGCGTGAAAGCCGCGAAGCAATTTGAGGAATTGGGTTCTTTTTATGATGTGTGTAAAAGTATTGGGGTTGGAAGAACCTCGGAATATCCGAGTGTTCAACAGCCTTCCGATGAGTTAGTCGCGACATTGCAGGAGTTTTTGAAAGATCATGCTTCCTTCCCCTGCCATGGAAATCGATCGCCGGCGGCGGCGGCGGCTCCGACGCTGATGCCGACGCCGACGAAGGTTTTTCTCTCTGGACCAACGGTGAAGTTCACCGACGAACAATCGGAATCGTCGGATAAGAATTCGGAGAGGGGGTCTGATTTTAACTCGTTTGGGGATATTAATTCCGTCGAGTATGACGGCGAGTGCTTCTCCGATTATCAGCAATCCCAGTTGGGAGATTTCAGTAATGAATCGATTCTGTCTCCGACTTTTGCGTTCTTTGAAGAAAACCAAAAGGGTTTTCAAGCGGAAGAACACCTATTTAGACTTGGGGATTGGGAGCTCGTTCTGGCGGAATCCGCTACCGCGCCGCAAAATTGGCCGGATTTCTACTCGCCGTTCGTGGGCGACAACATGTTTGTGAAGCCCTTTGCGCCGCAGCAGCAGCATCACAACAACAACCCATTTCTTCAAGATTCAAGCGATTTATTCACAGTTGCACCAACATTCAAAGCAGAGACGGAAATGACTCAAACATTTGGCGGTCATAATTTGAATGAAGCAACCACGATGGCCCCAACATTCAGAGCAGAAACAGCAACATTTCTGATTGGAGTTGATATGGGCATTGAAAACGATCCATTTGCAGTGCCGTGGGGAAGCGAATTGAGTGGAAATGATCGGAGTTTTGAAGTGATGAATCATGATAGTTTGCTGCAGCAGCAGAAATTGTGGAAGGAGCATCAAAACAAGATTATAGCGAAGCATTCGTTGTGA

Coding sequence (CDS)

ATGCCCAGCAAGTTAAGGAAGGCCATCTACGCCGTGAAAGATCAAACCAGCATCAGCCTTGCCAAAGTCTCCGCCTCCGGCAGCGGCCCCAATGCTACCTCCTTGGAAGTCACCATCCTCAAAGCCACCACTCACGACGACGTCCCTCTCGACCACCGTTACCTGGCGGAAATCCTCCTCTTCATCTCCTCCAACAAATCCCACGCCGCCGTCTGTGTTCATTCCATCGCCAAACGCATTACCAGAACCCGTAATTGGATCGTGGCCCTCAAATCTCTCAACCTCGTCTTCCGTATCTTCCAAGAAGGCGACCCGATTTTCCCCCGCGAAGTTCTCCTCGCCATGAAACGCGGCGCTAAAATCCTTAACCTCTCCAATTTCCGCGACGATTCAAATTCCAATCCGTGGGATTACACCGCTTTTGTTCGAACCTTTGCTCTGTATCTCGACGAACGCCTCGATTGCTTTCTTAACGGGAAGCTTCAAAAGCGATACACTCAAGGGAAGACTAGCCGACGGATGAACGAGCCGATTCGGGATATGAAGCCGACGATGTTAATTGATCGGATTTCTTACTGGCAGAAATTACTGGACAGAGCAATCGCCACTAGACCCACCGGATCGGCGAAGAGGAATCGATTGATTCAACATTCGCTTCACGCCGTCGTTCAAGAGAGCTTCGATTTATACAGAGATATCTCCGATGGGCTTGCGCTTCTCTTGGATAGCTTCTTCCATTTGCAGTACCAATCCTGTGTTAATGCGTTTCATGCGTGCGTGAAAGCCGCGAAGCAATTTGAGGAATTGGGTTCTTTTTATGATGTGTGTAAAAGTATTGGGGTTGGAAGAACCTCGGAATATCCGAGTGTTCAACAGCCTTCCGATGAGTTAGTCGCGACATTGCAGGAGTTTTTGAAAGATCATGCTTCCTTCCCCTGCCATGGAAATCGATCGCCGGCGGCGGCGGCGGCTCCGACGCTGATGCCGACGCCGACGAAGGTTTTTCTCTCTGGACCAACGGTGAAGTTCACCGACGAACAATCGGAATCGTCGGATAAGAATTCGGAGAGGGGGTCTGATTTTAACTCGTTTGGGGATATTAATTCCGTCGAGTATGACGGCGAGTGCTTCTCCGATTATCAGCAATCCCAGTTGGGAGATTTCAGTAATGAATCGATTCTGTCTCCGACTTTTGCGTTCTTTGAAGAAAACCAAAAGGGTTTTCAAGCGGAAGAACACCTATTTAGACTTGGGGATTGGGAGCTCGTTCTGGCGGAATCCGCTACCGCGCCGCAAAATTGGCCGGATTTCTACTCGCCGTTCGTGGGCGACAACATGTTTGTGAAGCCCTTTGCGCCGCAGCAGCAGCATCACAACAACAACCCATTTCTTCAAGATTCAAGCGATTTATTCACAGTTGCACCAACATTCAAAGCAGAGACGGAAATGACTCAAACATTTGGCGGTCATAATTTGAATGAAGCAACCACGATGGCCCCAACATTCAGAGCAGAAACAGCAACATTTCTGATTGGAGTTGATATGGGCATTGAAAACGATCCATTTGCAGTGCCGTGGGGAAGCGAATTGAGTGGAAATGATCGGAGTTTTGAAGTGATGAATCATGATAGTTTGCTGCAGCAGCAGAAATTGTGGAAGGAGCATCAAAACAAGATTATAGCGAAGCATTCGTTGTGA

Protein sequence

MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILLFISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAKILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVATLQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSERGSDFNSFGDINSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQKGFQAEEHLFRLGDWELVLAESATAPQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQDSSDLFTVAPTFKAETEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVPWGSELSGNDRSFEVMNHDSLLQQQKLWKEHQNKIIAKHSL
Homology
BLAST of CmaCh11G019310 vs. ExPASy Swiss-Prot
Match: Q9ZVN6 (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 7.5e-130
Identity = 317/682 (46.48%), Postives = 390/682 (57.18%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVS--ASGSGPNATSLEVTILKATTHD-DVPLDHRYLAE 60
           MPSKL+KAI AVKDQTSISLAKV+  A+G G + T+LEV ILKAT+HD +VP+D R + E
Sbjct: 1   MPSKLKKAIGAVKDQTSISLAKVANGATGGG-DLTTLEVAILKATSHDEEVPIDDRLVTE 60

Query: 61  ILLFISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKR 120
           IL  ISS KSHAA C  +I +RI RTRNWIVALKSL LV RIFQ+GDP FPREVL AMKR
Sbjct: 61  ILGIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKR 120

Query: 121 GAKILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRM--- 180
           GAKILNLS+FRDDSNS PWD+TAFVRTFALYLDERLDCFL GKLQ+RYT  + + R+   
Sbjct: 121 GAKILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTN 180

Query: 181 ---------------NEP-IRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHS 240
                          +EP +RDMKP ML+D+I+YWQKLLDRAIATRPTG AK NRL++ S
Sbjct: 181 STTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMS 240

Query: 241 LHAVVQESFDLYRDISDGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKS 300
           L+AV+QESFDLYRDISDGLALLLDSFFHLQYQSC+NAF ACV+A+KQFEEL +FYD+ KS
Sbjct: 241 LYAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKS 300

Query: 301 IGVGRTSEYPSVQQPSDELVATLQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSG 360
           IG+GRTSEYPS+Q+ S EL+ TLQEFLKD +SFP          A+  L P+P       
Sbjct: 301 IGIGRTSEYPSIQKISLELLETLQEFLKDQSSFP----------ASSGLYPSPNSFLPPP 360

Query: 361 PTVKFTDEQS-----ESSDKNSERGSDFNSF----------------------------- 420
           P+ K +   S     +S+   SER SD+ SF                             
Sbjct: 361 PSSKDSAVSSSLDFGDSTIDTSERYSDYGSFRSTSLEDLMSRTEAGTSSPPMSCHSEPYG 420

Query: 421 ---GDINSVEYDGECFSDYQQS-------------QLGDFSN-------------ESILS 480
               D N   +D         +              L D SN             +   +
Sbjct: 421 GGRDDPNGNNFDTVSTKSLPNNPSVSASNLILDLLSLDDVSNTAEAEDVEDKKKQDDSKA 480

Query: 481 PTFAFFEE--------------NQKGFQAEEHLFRLGDWELVLAESATAPQNWPDFYSPF 540
            TF  +E                ++   AE+H    G+W L L E+AT  Q         
Sbjct: 481 ETFDPWEALMLRDDPKKKIETIEEEPSTAEDHQRDSGNWLLALEETATQVQG-------- 540

Query: 541 VGDNMFVKPF-------APQQQHHNNNPFLQDSSDLFTVAPTFKAETEMTQTFGGHNLNE 564
             ++M + PF       A Q      NPFL++      VA    A   M  TFGG  L  
Sbjct: 541 -NNSMAIVPFGLDDPMPAFQAATDQYNPFLEE-----PVAQLATAGEPMI-TFGGLALT- 600

BLAST of CmaCh11G019310 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 267.3 bits (682), Expect = 3.9e-70
Identity = 155/385 (40.26%), Postives = 222/385 (57.66%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           M   +RKAI  VKDQTSI +AKV+++     A  LEV I+KAT+HDD     +Y+ EIL 
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDQSSDKYIREILS 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
             S ++ +   CV S+++R+ +TR+WIVALK+L LV R+  EGDP+F  E+L A +RG +
Sbjct: 61  LTSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTR 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFL--------NGKLQKRYTQG---- 180
           ILN+S+FRD+++S+ WD++AFVRT+A YLD+RL+  L         G      + G    
Sbjct: 121 ILNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGY 180

Query: 181 --------------------------KTSRRMNE--------------PIRDMKPTMLID 240
                                     K SR   +              P+R+M P  +  
Sbjct: 181 NRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFG 240

Query: 241 RISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALLLDSFFHLQ 300
           ++ + Q+LLDR ++ RPTG AK +R+I  +++ VV+ESF LY DI + LA+LLD FF ++
Sbjct: 241 KMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDME 300

Query: 301 YQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVATLQEFLKDH 330
           Y  CV AF A   AAKQ +EL +FY  CK  GV R+SEYP VQ+ + +L+ TL+EF++D 
Sbjct: 301 YTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDR 360

BLAST of CmaCh11G019310 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 266.9 bits (681), Expect = 5.1e-70
Identity = 153/399 (38.35%), Postives = 226/399 (56.64%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           M   +RKAI AVKDQTSI +AKV+++     A  LEV I+KAT+HDD P   +Y+ EIL 
Sbjct: 1   MAPSIRKAIGAVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDPASEKYIREILN 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
             S ++ +   CV S+++R+++TR+W+VALK+L LV R+  EGDPIF  E+L + +RG +
Sbjct: 61  LTSLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTR 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFL----------------------- 180
           +LN+S+FRD+++S+ WD++AFVRT+A YLD+RL+  L                       
Sbjct: 121 MLNMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDD 180

Query: 181 ---------------------------------NG-----KLQKRYTQ-----------G 240
                                            NG     K  + Y             G
Sbjct: 181 RYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGG 240

Query: 241 KTSRRMNEPIRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFD 300
           +  +++  P+R+M P  +  ++ + Q+LLDR ++ RPTG AK +R+I  +L+ VV+ESF 
Sbjct: 241 RDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFK 300

Query: 301 LYRDISDGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYP 328
           LY DI + LA+LLD FF ++Y  CV AF A   AAKQ +EL +FY+ CK  GV R+SEYP
Sbjct: 301 LYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYP 360

BLAST of CmaCh11G019310 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 259.2 bits (661), Expect = 1.1e-67
Identity = 127/339 (37.46%), Postives = 213/339 (62.83%), Query Frame = 0

Query: 3   SKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILLFI 62
           SKL++AI AVKDQTS+ LAKV   G   + T LE+ ++KAT HDD P + +Y+ EIL   
Sbjct: 4   SKLKRAIGAVKDQTSVGLAKV--GGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCLT 63

Query: 63  SSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAKIL 122
           S ++++ + CV ++++R+ +T+NW VALK+L L+ R+  +GD  + +E+  A +RG ++L
Sbjct: 64  SYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRLL 123

Query: 123 NLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQG-------------- 182
           N+S+FRD S S+ WDY+AFVRT+ALYLDERLD  + G+  K+ + G              
Sbjct: 124 NMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDDH 183

Query: 183 ---------KTSRRMNEPIRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSL 242
                    K     ++P+ +MK   + +R+ + Q+LLDR +A RPTG+AK NR++  ++
Sbjct: 184 RGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVAM 243

Query: 243 HAVVQESFDLYRDISDGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSI 302
           + +V+ESF LY +I++ + +L++ F  L     +  +    + +KQF+EL  FY  CK++
Sbjct: 244 YPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNM 303

Query: 303 GVGRTSEYPSVQQPSDELVATLQEFLKDHASFPCHGNRS 319
            V R+SEYP +++ + + +  + EF++D ++      +S
Sbjct: 304 AVARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKS 340

BLAST of CmaCh11G019310 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 3.4e-66
Identity = 127/326 (38.96%), Postives = 208/326 (63.80%), Query Frame = 0

Query: 3   SKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILLFI 62
           SK ++AI AVKDQTS+ LAKV  +G   + + L+V I+KAT H++ P + +Y+ EIL   
Sbjct: 4   SKFKRAIGAVKDQTSVGLAKV--NGRSASLSELDVAIVKATRHEEFPAEEKYIREILSLT 63

Query: 63  SSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAKIL 122
           S ++S+   CV ++++R+ +T+ W VALK+L L+ R+  EGD  + +E+  A +RG ++L
Sbjct: 64  SYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRLL 123

Query: 123 NLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKR--YTQGKTSRR------- 182
           N+S+FRD S SN WDY+AFVRT+ALYLDERLD  +  +  KR  Y  G  +         
Sbjct: 124 NMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVYCVGGEADEEEQDQAA 183

Query: 183 ---------MNEPIRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQ 242
                     ++PI +MK   +  RI + Q+LLDR +A RPTG+A+ NR++  +L+ +V+
Sbjct: 184 ADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTGNARNNRVVIVALYPIVK 243

Query: 243 ESFDLYRDISDGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRT 302
           ESF +Y D+++ + +L++ F  L     +  +    + +KQFEEL  FY  CK++G+ R+
Sbjct: 244 ESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARS 303

Query: 303 SEYPSVQQPSDELVATLQEFLKDHAS 311
           SEYP +++ + + +  + EF++D ++
Sbjct: 304 SEYPEIEKITQKKLDLMDEFIRDKSA 327

BLAST of CmaCh11G019310 vs. ExPASy TrEMBL
Match: A0A6J1JET6 (clathrin coat assembly protein AP180-like OS=Cucurbita maxima OX=3661 GN=LOC111486264 PE=4 SV=1)

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL
Sbjct: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
           FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK
Sbjct: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180
           ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD
Sbjct: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180

Query: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240
           MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL
Sbjct: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240

Query: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT 300
           LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT
Sbjct: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT 300

Query: 301 LQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSERGSD 360
           LQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSERGSD
Sbjct: 301 LQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSERGSD 360

Query: 361 FNSFGDINSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQKGFQAEEHLFRLGDW 420
           FNSFGDINSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQKGFQAEEHLFRLGDW
Sbjct: 361 FNSFGDINSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQKGFQAEEHLFRLGDW 420

Query: 421 ELVLAESATAPQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQDSSDLFTVAPTFKAE 480
           ELVLAESATAPQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQDSSDLFTVAPTFKAE
Sbjct: 421 ELVLAESATAPQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQDSSDLFTVAPTFKAE 480

Query: 481 TEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVPWGSELSGNDRSFEVM 540
           TEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVPWGSELSGNDRSFEVM
Sbjct: 481 TEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVPWGSELSGNDRSFEVM 540

Query: 541 NHDSLLQQQKLWKEHQNKIIAKHSL 566
           NHDSLLQQQKLWKEHQNKIIAKHSL
Sbjct: 541 NHDSLLQQQKLWKEHQNKIIAKHSL 565

BLAST of CmaCh11G019310 vs. ExPASy TrEMBL
Match: A0A6J1ESQ2 (clathrin coat assembly protein AP180-like OS=Cucurbita moschata OX=3662 GN=LOC111437220 PE=4 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.2e-303
Identity = 530/577 (91.85%), Postives = 540/577 (93.59%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSK RKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL
Sbjct: 1   MPSKFRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
           FISSNKSHAAVCVHSIAKRITRTRNW+VALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK
Sbjct: 61  FISSNKSHAAVCVHSIAKRITRTRNWVVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180
           ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIR 
Sbjct: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRY 180

Query: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240
           MKPTMLIDRIS+WQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL
Sbjct: 181 MKPTMLIDRISHWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240

Query: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT 300
           LDSFFHLQ+QSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDEL+ T
Sbjct: 241 LDSFFHLQHQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELIET 300

Query: 301 LQEFLKDHASFPCHGNRS-PAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSERGS 360
           LQEFLKDHASFPCHGNRS P     P  MPTPT+ FLSGPTVKF DEQSESSDKNS+ GS
Sbjct: 301 LQEFLKDHASFPCHGNRSPPTPTPTPMPMPTPTQDFLSGPTVKFNDEQSESSDKNSDWGS 360

Query: 361 DFNSFGDI-----------NSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQKGF 420
           DFNSFGDI           NSVEYDGECFSD +QSQ GDF+NESILSPTF FFEENQK F
Sbjct: 361 DFNSFGDIMTAAGSWPSPANSVEYDGECFSDNRQSQSGDFTNESILSPTFPFFEENQKRF 420

Query: 421 QAEEHLFRLGDWELVLAESATAPQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQDSS 480
           QAEEH  RLGDWELVLAESAT PQNWPDF+SP VGDNMF+KPF PQQ HHNNNPFLQDSS
Sbjct: 421 QAEEHQLRLGDWELVLAESAT-PQNWPDFFSPSVGDNMFMKPFTPQQHHHNNNPFLQDSS 480

Query: 481 DLFTVAPTFKAETEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVPWGS 540
           DLFTVAPTFKAETEMTQTFGGHNLNEA TMAPTFRAETATFLIGVDMGIENDPFAVPWGS
Sbjct: 481 DLFTVAPTFKAETEMTQTFGGHNLNEAATMAPTFRAETATFLIGVDMGIENDPFAVPWGS 540

Query: 541 ELSGNDRSFEVMNHDSLLQQQKLWKEHQNKIIAKHSL 566
           ELSGNDRSFE MNHDSLLQQQKLWKEHQNKIIAKHSL
Sbjct: 541 ELSGNDRSFEGMNHDSLLQQQKLWKEHQNKIIAKHSL 576

BLAST of CmaCh11G019310 vs. ExPASy TrEMBL
Match: A0A5D3C685 (Clathrin coat assembly protein AP180 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00110 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.4e-232
Identity = 440/599 (73.46%), Postives = 474/599 (79.13%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSKLRKA+YAVKDQTSI LAKVS+S  GPNATSLEV +LKATTHDDVPLDHRY+ EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVAVLKATTHDDVPLDHRYVNEILT 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
            IS+NKSHAA C HSIAKRIT+TRNW VALKSLNLV +IFQ+GDP FPREVL AMKRGAK
Sbjct: 61  LISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGK------TSRRM 180
           ILNLSNFRDDSNS+PWDYTAFVRTFALYLDERLDCFL GKLQKR+TQ K      T+RR+
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHATTRRI 180

Query: 181 NEPIRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDIS 240
           NEPIRDMKP MLIDRI+YWQKLLDRAIATRPTG AK NRL+QHSLHAVVQESFDLYRDIS
Sbjct: 181 NEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAF ACVKAAKQFEELGSFYD+CKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELVATLQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTD--EQSESSD 360
           DEL+ TLQEFLKD ASFPCHGNRSP  A             LS P  K TD  EQSESSD
Sbjct: 301 DELIETLQEFLKDQASFPCHGNRSPPQA------------ILSVPMTKLTDDLEQSESSD 360

Query: 361 KNSERGSDFNSFGDI-----------NSVEYDGECFSDYQ---QSQLGDFSNESILSPTF 420
           KNSERGSDFNS GDI           NSVE DGE +SDYQ   QS+LGDF+NES LSP F
Sbjct: 361 KNSERGSDFNSVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNF 420

Query: 421 AFFEENQKGFQAEEHL-----FRLGDWELVLAESATA-PQNWPDFYSPFVGDNMFVKPFA 480
           AFFEEN   F+ E         R GDWELVLAESATA P+ WPDF+SP +GD++F KPF+
Sbjct: 421 AFFEENGMPFEEESQFGVAPSLRFGDWELVLAESATASPKEWPDFFSPSIGDDLFAKPFS 480

Query: 481 PQQQHHNNNPFLQDSSDLFTVAPTFKAETEMTQTFGGHNLNEATTMAPTFRAETATFLI- 540
           P  QHH  NPFLQDS DLFT  PTFKAE EM  TF   N     ++APTFRAETAT    
Sbjct: 481 P--QHHYKNPFLQDSDDLFTAPPTFKAEKEMGPTFRAEN-----SVAPTFRAETATLSSQ 540

Query: 541 ----GVD-MGIENDPFAVPWGSELSGNDRSFEVMNHDSLLQQQKLWKEHQNKIIAKHSL 566
               G D +G  NDPFA P    L+GND+SF+    +SLLQQQKLWKE QNKIIAKH+L
Sbjct: 541 NENRGFDELGFGNDPFAGP----LNGNDQSFD---EESLLQQQKLWKEQQNKIIAKHTL 573

BLAST of CmaCh11G019310 vs. ExPASy TrEMBL
Match: A0A1S3BN17 (clathrin coat assembly protein AP180 OS=Cucumis melo OX=3656 GN=LOC103491352 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.4e-232
Identity = 440/599 (73.46%), Postives = 474/599 (79.13%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSKLRKA+YAVKDQTSI LAKVS+S  GPNATSLEV +LKATTHDDVPLDHRY+ EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVAVLKATTHDDVPLDHRYVNEILT 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
            IS+NKSHAA C HSIAKRIT+TRNW VALKSLNLV +IFQ+GDP FPREVL AMKRGAK
Sbjct: 61  LISANKSHAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGK------TSRRM 180
           ILNLSNFRDDSNS+PWDYTAFVRTFALYLDERLDCFL GKLQKR+TQ K      T+RR+
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKTESYHATTRRI 180

Query: 181 NEPIRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDIS 240
           NEPIRDMKP MLIDRI+YWQKLLDRAIATRPTG AK NRL+QHSLHAVVQESFDLYRDIS
Sbjct: 181 NEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAF ACVKAAKQFEELGSFYD+CKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELVATLQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTD--EQSESSD 360
           DEL+ TLQEFLKD ASFPCHGNRSP  A             LS P  K TD  EQSESSD
Sbjct: 301 DELIETLQEFLKDQASFPCHGNRSPPQA------------ILSVPMTKLTDDLEQSESSD 360

Query: 361 KNSERGSDFNSFGDI-----------NSVEYDGECFSDYQ---QSQLGDFSNESILSPTF 420
           KNSERGSDFNS GDI           NSVE DGE +SDYQ   QS+LGDF+NES LSP F
Sbjct: 361 KNSERGSDFNSVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANESNLSPNF 420

Query: 421 AFFEENQKGFQAEEHL-----FRLGDWELVLAESATA-PQNWPDFYSPFVGDNMFVKPFA 480
           AFFEEN   F+ E         R GDWELVLAESATA P+ WPDF+SP +GD++F KPF+
Sbjct: 421 AFFEENGMPFEEESQFGVAPSLRFGDWELVLAESATASPKEWPDFFSPSIGDDLFAKPFS 480

Query: 481 PQQQHHNNNPFLQDSSDLFTVAPTFKAETEMTQTFGGHNLNEATTMAPTFRAETATFLI- 540
           P  QHH  NPFLQDS DLFT  PTFKAE EM  TF   N     ++APTFRAETAT    
Sbjct: 481 P--QHHYKNPFLQDSDDLFTAPPTFKAEKEMGPTFRAEN-----SVAPTFRAETATLSSQ 540

Query: 541 ----GVD-MGIENDPFAVPWGSELSGNDRSFEVMNHDSLLQQQKLWKEHQNKIIAKHSL 566
               G D +G  NDPFA P    L+GND+SF+    +SLLQQQKLWKE QNKIIAKH+L
Sbjct: 541 NENRGFDELGFGNDPFAGP----LNGNDQSFD---EESLLQQQKLWKEQQNKIIAKHTL 573

BLAST of CmaCh11G019310 vs. ExPASy TrEMBL
Match: A0A0A0KGJ5 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421580 PE=4 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 3.6e-228
Identity = 433/599 (72.29%), Postives = 470/599 (78.46%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSKLRKA+YAVKDQTSI LAKVS+S  GPNATSLEV +LKATTHDD PLDHRY+ EIL 
Sbjct: 1   MPSKLRKALYAVKDQTSIGLAKVSSSAGGPNATSLEVVVLKATTHDDSPLDHRYVTEILT 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
            IS+NKS+AA C HSIAKRIT+TRNW VALKSLNLV +IFQ+GDP FPREVL AMKRGAK
Sbjct: 61  LISANKSNAAACAHSIAKRITKTRNWTVALKSLNLVLKIFQDGDPYFPREVLHAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGK------TSRRM 180
           ILNLSNFRDDSNS+PWDYTAFVRTFALYLDERLDCFL GKLQKR+TQ K      T+RR+
Sbjct: 121 ILNLSNFRDDSNSSPWDYTAFVRTFALYLDERLDCFLTGKLQKRFTQRKAESYHATTRRI 180

Query: 181 NEPIRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDIS 240
           NEPIRDMKP MLIDRI+YWQKLLDRAIATRPTG AK NRL+QHSLHAVVQESFDLYRDIS
Sbjct: 181 NEPIRDMKPAMLIDRITYWQKLLDRAIATRPTGPAKGNRLVQHSLHAVVQESFDLYRDIS 240

Query: 241 DGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPS 300
           DGLALLLDSFFHLQYQSCVNAF ACVKAAKQFEELGSFYD+CKSIGVGRTSEYPSVQQPS
Sbjct: 241 DGLALLLDSFFHLQYQSCVNAFQACVKAAKQFEELGSFYDLCKSIGVGRTSEYPSVQQPS 300

Query: 301 DELVATLQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTD--EQSESSD 360
           DEL+ TLQEFLKD ASFPCHGNRSP         P P    L G   KFTD  +QSESSD
Sbjct: 301 DELIETLQEFLKDQASFPCHGNRSP---------PQP---ILPGSITKFTDDLDQSESSD 360

Query: 361 KNSERGSDFNSFGDI-----------NSVEYDGECFSDYQ---QSQLGDFSNESILSPTF 420
           KNSERGSDFNS GDI           NSVE DGE +SDYQ   QS+LGDF+NE  LSP F
Sbjct: 361 KNSERGSDFNSVGDIMSATGSWTSPANSVEQDGEGYSDYQSEKQSRLGDFANELNLSPNF 420

Query: 421 AFFEENQKGFQAEEHL-----FRLGDWELVLAESAT-APQNWPDFYSPFVGDNMFVKPFA 480
           AFFEE+   F+ E         R GDWELVLAESAT +P+ WPDF+SP +GD ++ KPF+
Sbjct: 421 AFFEESGMAFEEESQFGVAPSMRFGDWELVLAESATDSPKEWPDFFSPSIGDELYAKPFS 480

Query: 481 PQQQHHNNNPFLQDSSDLFTVAPTFKAETEMTQTFGGHNLNEATTMAPTFRAETATFL-- 540
           P  QHH  NPFLQDS DLFT  PTFKAE EM  TF   N     ++APTFRAETAT    
Sbjct: 481 P--QHHYKNPFLQDSDDLFTAPPTFKAEKEMGPTFRAEN-----SVAPTFRAETATLSSQ 540

Query: 541 ---IGVD-MGIENDPFAVPWGSELSGNDRSFEVMNHDSLLQQQKLWKEHQNKIIAKHSL 566
               G D +G  NDPFA P    L+GND  F+    +SLLQQQKLWKE QNKIIAKH+L
Sbjct: 541 NENCGFDELGFGNDPFAGP----LNGNDPIFD---EESLLQQQKLWKEQQNKIIAKHTL 573

BLAST of CmaCh11G019310 vs. NCBI nr
Match: XP_022989087.1 (clathrin coat assembly protein AP180-like [Cucurbita maxima])

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL
Sbjct: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
           FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK
Sbjct: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180
           ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD
Sbjct: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180

Query: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240
           MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL
Sbjct: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240

Query: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT 300
           LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT
Sbjct: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT 300

Query: 301 LQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSERGSD 360
           LQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSERGSD
Sbjct: 301 LQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSERGSD 360

Query: 361 FNSFGDINSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQKGFQAEEHLFRLGDW 420
           FNSFGDINSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQKGFQAEEHLFRLGDW
Sbjct: 361 FNSFGDINSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQKGFQAEEHLFRLGDW 420

Query: 421 ELVLAESATAPQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQDSSDLFTVAPTFKAE 480
           ELVLAESATAPQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQDSSDLFTVAPTFKAE
Sbjct: 421 ELVLAESATAPQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQDSSDLFTVAPTFKAE 480

Query: 481 TEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVPWGSELSGNDRSFEVM 540
           TEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVPWGSELSGNDRSFEVM
Sbjct: 481 TEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVPWGSELSGNDRSFEVM 540

Query: 541 NHDSLLQQQKLWKEHQNKIIAKHSL 566
           NHDSLLQQQKLWKEHQNKIIAKHSL
Sbjct: 541 NHDSLLQQQKLWKEHQNKIIAKHSL 565

BLAST of CmaCh11G019310 vs. NCBI nr
Match: XP_023531390.1 (clathrin coat assembly protein AP180-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.4e-306
Identity = 534/580 (92.07%), Postives = 545/580 (93.97%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSK RKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL
Sbjct: 1   MPSKFRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
           FISSNKSHAAVCVHSIAKRI RTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK
Sbjct: 61  FISSNKSHAAVCVHSIAKRIARTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180
           ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKT+RRMNEPIRD
Sbjct: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTTRRMNEPIRD 180

Query: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240
           MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL
Sbjct: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240

Query: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT 300
           LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDEL+ T
Sbjct: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELIET 300

Query: 301 LQEFLKDHASFPCHGNRS---PAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSER 360
           LQEFLK+HASFPCHGNRS   P     P   PTPT+VFLSGPTVKFT+EQ ESSDKNS+ 
Sbjct: 301 LQEFLKEHASFPCHGNRSPPPPTPMPMPMPTPTPTQVFLSGPTVKFTEEQLESSDKNSDW 360

Query: 361 GSDFNSFGDI-----------NSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQK 420
           GSDFNSFGDI           NSVE+DGECFSDYQQSQLGDFSNESILSP FAFFEENQK
Sbjct: 361 GSDFNSFGDIMSAAGSWPSPANSVEHDGECFSDYQQSQLGDFSNESILSPNFAFFEENQK 420

Query: 421 GFQAEEHLFRLGDWELVLAESAT-APQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQ 480
           GFQAEEH FR+GDWE+VLAESAT +PQNWPDF+SP VGDNMFVKP APQQQHHNNNPFLQ
Sbjct: 421 GFQAEEHQFRVGDWEIVLAESATESPQNWPDFFSPSVGDNMFVKPLAPQQQHHNNNPFLQ 480

Query: 481 DSSDLFTVAPTFKAETEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVP 540
           DSSDLFTVAPTFKAETEMTQTFG HNLNEA TMAPTFRAETATFLIGVDMGIENDPFAVP
Sbjct: 481 DSSDLFTVAPTFKAETEMTQTFGAHNLNEAATMAPTFRAETATFLIGVDMGIENDPFAVP 540

Query: 541 WGSELSGNDRSFEVMNHDSLLQQQKLWKEHQNKIIAKHSL 566
           WGSELSGNDRSFE MNHDSLLQQQKLWKEHQNKIIAKHSL
Sbjct: 541 WGSELSGNDRSFEGMNHDSLLQQQKLWKEHQNKIIAKHSL 580

BLAST of CmaCh11G019310 vs. NCBI nr
Match: XP_022930864.1 (clathrin coat assembly protein AP180-like [Cucurbita moschata])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.4e-303
Identity = 530/577 (91.85%), Postives = 540/577 (93.59%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSK RKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL
Sbjct: 1   MPSKFRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
           FISSNKSHAAVCVHSIAKRITRTRNW+VALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK
Sbjct: 61  FISSNKSHAAVCVHSIAKRITRTRNWVVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180
           ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIR 
Sbjct: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRY 180

Query: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240
           MKPTMLIDRIS+WQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL
Sbjct: 181 MKPTMLIDRISHWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240

Query: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT 300
           LDSFFHLQ+QSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDEL+ T
Sbjct: 241 LDSFFHLQHQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELIET 300

Query: 301 LQEFLKDHASFPCHGNRS-PAAAAAPTLMPTPTKVFLSGPTVKFTDEQSESSDKNSERGS 360
           LQEFLKDHASFPCHGNRS P     P  MPTPT+ FLSGPTVKF DEQSESSDKNS+ GS
Sbjct: 301 LQEFLKDHASFPCHGNRSPPTPTPTPMPMPTPTQDFLSGPTVKFNDEQSESSDKNSDWGS 360

Query: 361 DFNSFGDI-----------NSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQKGF 420
           DFNSFGDI           NSVEYDGECFSD +QSQ GDF+NESILSPTF FFEENQK F
Sbjct: 361 DFNSFGDIMTAAGSWPSPANSVEYDGECFSDNRQSQSGDFTNESILSPTFPFFEENQKRF 420

Query: 421 QAEEHLFRLGDWELVLAESATAPQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQDSS 480
           QAEEH  RLGDWELVLAESAT PQNWPDF+SP VGDNMF+KPF PQQ HHNNNPFLQDSS
Sbjct: 421 QAEEHQLRLGDWELVLAESAT-PQNWPDFFSPSVGDNMFMKPFTPQQHHHNNNPFLQDSS 480

Query: 481 DLFTVAPTFKAETEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVPWGS 540
           DLFTVAPTFKAETEMTQTFGGHNLNEA TMAPTFRAETATFLIGVDMGIENDPFAVPWGS
Sbjct: 481 DLFTVAPTFKAETEMTQTFGGHNLNEAATMAPTFRAETATFLIGVDMGIENDPFAVPWGS 540

Query: 541 ELSGNDRSFEVMNHDSLLQQQKLWKEHQNKIIAKHSL 566
           ELSGNDRSFE MNHDSLLQQQKLWKEHQNKIIAKHSL
Sbjct: 541 ELSGNDRSFEGMNHDSLLQQQKLWKEHQNKIIAKHSL 576

BLAST of CmaCh11G019310 vs. NCBI nr
Match: KAG7022969.1 (Clathrin coat assembly protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.2e-303
Identity = 529/580 (91.21%), Postives = 541/580 (93.28%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSK RKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLA+ILL
Sbjct: 1   MPSKFRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLADILL 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
           FISSNKSHAAVCVHSIAKRITRTRNW+VALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK
Sbjct: 61  FISSNKSHAAVCVHSIAKRITRTRNWVVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180
           ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD
Sbjct: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180

Query: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240
           MKPTMLIDRIS+WQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL
Sbjct: 181 MKPTMLIDRISHWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240

Query: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT 300
           LDSFFHLQYQSCV+AFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQ+PSDEL+ T
Sbjct: 241 LDSFFHLQYQSCVSAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQRPSDELIET 300

Query: 301 LQEFLKDHASFPCHGNRSPAAAA---APTLMPTPTKVFLSGPTVKFTDEQSESSDKNSER 360
           LQEFLK HASFPCHGNRSP        PT  PTPT+ FLSGPTVKFTDEQSESSDKNS+ 
Sbjct: 301 LQEFLKHHASFPCHGNRSPPTPTPMPMPTPTPTPTQDFLSGPTVKFTDEQSESSDKNSDW 360

Query: 361 GSDFNSFGDI-----------NSVEYDGECFSDYQQSQLGDFSNESILSPTFAFFEENQK 420
           GSDFNSFGDI           NSVEYDGECFSD +QSQ GDF+NE ILSPTF FFEENQK
Sbjct: 361 GSDFNSFGDIMSAAGSWPSPANSVEYDGECFSDNRQSQSGDFTNELILSPTFPFFEENQK 420

Query: 421 GFQAEEHLFRLGDWELVLAESAT-APQNWPDFYSPFVGDNMFVKPFAPQQQHHNNNPFLQ 480
           GFQAEEH  RLGDWELVLAESAT +PQNWPDF+SP VGDNMFVKPF PQQ HHNNNPFLQ
Sbjct: 421 GFQAEEHQLRLGDWELVLAESATESPQNWPDFFSPSVGDNMFVKPFTPQQHHHNNNPFLQ 480

Query: 481 DSSDLFTVAPTFKAETEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGIENDPFAVP 540
           DSSDLFTVAPTFKAETEMT TFGGHNLNEA TMAPTFRAETATFLIGVDMGIENDPFAVP
Sbjct: 481 DSSDLFTVAPTFKAETEMTHTFGGHNLNEAATMAPTFRAETATFLIGVDMGIENDPFAVP 540

Query: 541 WGSELSGNDRSFEVMNHDSLLQQQKLWKEHQNKIIAKHSL 566
           WGSELSGNDRSFE MNHDSLLQQQKLWKEHQNKIIAKHSL
Sbjct: 541 WGSELSGNDRSFEGMNHDSLLQQQKLWKEHQNKIIAKHSL 580

BLAST of CmaCh11G019310 vs. NCBI nr
Match: KAG6589271.1 (Clathrin coat assembly protein 180, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.7e-302
Identity = 529/588 (89.97%), Postives = 541/588 (92.01%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           MPSK RKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLA+ILL
Sbjct: 1   MPSKFRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLADILL 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
           FISSNKSHAAVCVHSIAKRITRTRNW+VALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK
Sbjct: 61  FISSNKSHAAVCVHSIAKRITRTRNWVVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180
           ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD
Sbjct: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRMNEPIRD 180

Query: 181 MKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240
           MKPTMLIDRIS+WQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL
Sbjct: 181 MKPTMLIDRISHWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALL 240

Query: 241 LDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVAT 300
           LDSFFHLQYQSCV+AFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQ+PSDEL+ T
Sbjct: 241 LDSFFHLQYQSCVSAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQRPSDELIET 300

Query: 301 LQEFLKDHASFPCHGNRSPAAAA-----------APTLMPTPTKVFLSGPTVKFTDEQSE 360
           LQEFLK HASFPCHGNRSP                PT  PTPT+ FLSGPTVKFTDEQSE
Sbjct: 301 LQEFLKHHASFPCHGNRSPPTPTPTPTPTPTPMPMPTPTPTPTQDFLSGPTVKFTDEQSE 360

Query: 361 SSDKNSERGSDFNSFGDI-----------NSVEYDGECFSDYQQSQLGDFSNESILSPTF 420
           SSDKNS+ GSDFNSFGDI           NSVEYDGECFSD +QSQ GDF+NE ILSPTF
Sbjct: 361 SSDKNSDWGSDFNSFGDIMSAAGSWPSPANSVEYDGECFSDNRQSQSGDFTNELILSPTF 420

Query: 421 AFFEENQKGFQAEEHLFRLGDWELVLAESAT-APQNWPDFYSPFVGDNMFVKPFAPQQQH 480
            FFEENQKGFQAEEH  RLGDWELVLAESAT +PQNWPDF+SP VGDNMFVKPF PQQ H
Sbjct: 421 PFFEENQKGFQAEEHQLRLGDWELVLAESATESPQNWPDFFSPSVGDNMFVKPFTPQQHH 480

Query: 481 HNNNPFLQDSSDLFTVAPTFKAETEMTQTFGGHNLNEATTMAPTFRAETATFLIGVDMGI 540
           HNNNPFLQDSSDLFTVAPTFKAETEMT TFGGHNLNEA TMAPTFRAETATFLIGVDMGI
Sbjct: 481 HNNNPFLQDSSDLFTVAPTFKAETEMTHTFGGHNLNEAATMAPTFRAETATFLIGVDMGI 540

Query: 541 ENDPFAVPWGSELSGNDRSFEVMNHDSLLQQQKLWKEHQNKIIAKHSL 566
           ENDPFAVPWGSELSGNDRSFE MNHDSLLQQQKLWKEHQNKIIAKHSL
Sbjct: 541 ENDPFAVPWGSELSGNDRSFEGMNHDSLLQQQKLWKEHQNKIIAKHSL 588

BLAST of CmaCh11G019310 vs. TAIR 10
Match: AT1G05020.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 465.7 bits (1197), Expect = 5.3e-131
Identity = 317/682 (46.48%), Postives = 390/682 (57.18%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVS--ASGSGPNATSLEVTILKATTHD-DVPLDHRYLAE 60
           MPSKL+KAI AVKDQTSISLAKV+  A+G G + T+LEV ILKAT+HD +VP+D R + E
Sbjct: 1   MPSKLKKAIGAVKDQTSISLAKVANGATGGG-DLTTLEVAILKATSHDEEVPIDDRLVTE 60

Query: 61  ILLFISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKR 120
           IL  ISS KSHAA C  +I +RI RTRNWIVALKSL LV RIFQ+GDP FPREVL AMKR
Sbjct: 61  ILGIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKR 120

Query: 121 GAKILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQGKTSRRM--- 180
           GAKILNLS+FRDDSNS PWD+TAFVRTFALYLDERLDCFL GKLQ+RYT  + + R+   
Sbjct: 121 GAKILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTGRISTN 180

Query: 181 ---------------NEP-IRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHS 240
                          +EP +RDMKP ML+D+I+YWQKLLDRAIATRPTG AK NRL++ S
Sbjct: 181 STTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDAKANRLVKMS 240

Query: 241 LHAVVQESFDLYRDISDGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKS 300
           L+AV+QESFDLYRDISDGLALLLDSFFHLQYQSC+NAF ACV+A+KQFEEL +FYD+ KS
Sbjct: 241 LYAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVRASKQFEELNAFYDLSKS 300

Query: 301 IGVGRTSEYPSVQQPSDELVATLQEFLKDHASFPCHGNRSPAAAAAPTLMPTPTKVFLSG 360
           IG+GRTSEYPS+Q+ S EL+ TLQEFLKD +SFP          A+  L P+P       
Sbjct: 301 IGIGRTSEYPSIQKISLELLETLQEFLKDQSSFP----------ASSGLYPSPNSFLPPP 360

Query: 361 PTVKFTDEQS-----ESSDKNSERGSDFNSF----------------------------- 420
           P+ K +   S     +S+   SER SD+ SF                             
Sbjct: 361 PSSKDSAVSSSLDFGDSTIDTSERYSDYGSFRSTSLEDLMSRTEAGTSSPPMSCHSEPYG 420

Query: 421 ---GDINSVEYDGECFSDYQQS-------------QLGDFSN-------------ESILS 480
               D N   +D         +              L D SN             +   +
Sbjct: 421 GGRDDPNGNNFDTVSTKSLPNNPSVSASNLILDLLSLDDVSNTAEAEDVEDKKKQDDSKA 480

Query: 481 PTFAFFEE--------------NQKGFQAEEHLFRLGDWELVLAESATAPQNWPDFYSPF 540
            TF  +E                ++   AE+H    G+W L L E+AT  Q         
Sbjct: 481 ETFDPWEALMLRDDPKKKIETIEEEPSTAEDHQRDSGNWLLALEETATQVQG-------- 540

Query: 541 VGDNMFVKPF-------APQQQHHNNNPFLQDSSDLFTVAPTFKAETEMTQTFGGHNLNE 564
             ++M + PF       A Q      NPFL++      VA    A   M  TFGG  L  
Sbjct: 541 -NNSMAIVPFGLDDPMPAFQAATDQYNPFLEE-----PVAQLATAGEPMI-TFGGLALT- 600

BLAST of CmaCh11G019310 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 2.8e-71
Identity = 155/385 (40.26%), Postives = 222/385 (57.66%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           M   +RKAI  VKDQTSI +AKV+++     A  LEV I+KAT+HDD     +Y+ EIL 
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDQSSDKYIREILS 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
             S ++ +   CV S+++R+ +TR+WIVALK+L LV R+  EGDP+F  E+L A +RG +
Sbjct: 61  LTSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTR 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFL--------NGKLQKRYTQG---- 180
           ILN+S+FRD+++S+ WD++AFVRT+A YLD+RL+  L         G      + G    
Sbjct: 121 ILNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGY 180

Query: 181 --------------------------KTSRRMNE--------------PIRDMKPTMLID 240
                                     K SR   +              P+R+M P  +  
Sbjct: 181 NRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFG 240

Query: 241 RISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALLLDSFFHLQ 300
           ++ + Q+LLDR ++ RPTG AK +R+I  +++ VV+ESF LY DI + LA+LLD FF ++
Sbjct: 241 KMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDME 300

Query: 301 YQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVATLQEFLKDH 330
           Y  CV AF A   AAKQ +EL +FY  CK  GV R+SEYP VQ+ + +L+ TL+EF++D 
Sbjct: 301 YTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDR 360

BLAST of CmaCh11G019310 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 2.8e-71
Identity = 155/385 (40.26%), Postives = 222/385 (57.66%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           M   +RKAI  VKDQTSI +AKV+++     A  LEV I+KAT+HDD     +Y+ EIL 
Sbjct: 1   MALSMRKAIGVVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDQSSDKYIREILS 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
             S ++ +   CV S+++R+ +TR+WIVALK+L LV R+  EGDP+F  E+L A +RG +
Sbjct: 61  LTSLSRGYVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTR 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFL--------NGKLQKRYTQG---- 180
           ILN+S+FRD+++S+ WD++AFVRT+A YLD+RL+  L         G      + G    
Sbjct: 121 ILNMSDFRDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNGGGSSSSHQSNGDDGY 180

Query: 181 --------------------------KTSRRMNE--------------PIRDMKPTMLID 240
                                     K SR   +              P+R+M P  +  
Sbjct: 181 NRSRDDFRSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFG 240

Query: 241 RISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFDLYRDISDGLALLLDSFFHLQ 300
           ++ + Q+LLDR ++ RPTG AK +R+I  +++ VV+ESF LY DI + LA+LLD FF ++
Sbjct: 241 KMGHLQRLLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDME 300

Query: 301 YQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYPSVQQPSDELVATLQEFLKDH 330
           Y  CV AF A   AAKQ +EL +FY  CK  GV R+SEYP VQ+ + +L+ TL+EF++D 
Sbjct: 301 YTDCVKAFDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDR 360

BLAST of CmaCh11G019310 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 266.9 bits (681), Expect = 3.6e-71
Identity = 153/399 (38.35%), Postives = 226/399 (56.64%), Query Frame = 0

Query: 1   MPSKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILL 60
           M   +RKAI AVKDQTSI +AKV+++     A  LEV I+KAT+HDD P   +Y+ EIL 
Sbjct: 1   MAPSIRKAIGAVKDQTSIGIAKVASN----MAPDLEVAIVKATSHDDDPASEKYIREILN 60

Query: 61  FISSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAK 120
             S ++ +   CV S+++R+++TR+W+VALK+L LV R+  EGDPIF  E+L + +RG +
Sbjct: 61  LTSLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTR 120

Query: 121 ILNLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFL----------------------- 180
           +LN+S+FRD+++S+ WD++AFVRT+A YLD+RL+  L                       
Sbjct: 121 MLNMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSGGNSSHHSNNDD 180

Query: 181 ---------------------------------NG-----KLQKRYTQ-----------G 240
                                            NG     K  + Y             G
Sbjct: 181 RYGRGRDDFRSPPPRSYDYENGGGGGSDFRGDNNGYGGVPKRSRSYGDMTEMGGGGGGGG 240

Query: 241 KTSRRMNEPIRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSLHAVVQESFD 300
           +  +++  P+R+M P  +  ++ + Q+LLDR ++ RPTG AK +R+I  +L+ VV+ESF 
Sbjct: 241 RDEKKVVTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFK 300

Query: 301 LYRDISDGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSIGVGRTSEYP 328
           LY DI + LA+LLD FF ++Y  CV AF A   AAKQ +EL +FY+ CK  GV R+SEYP
Sbjct: 301 LYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYP 360

BLAST of CmaCh11G019310 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 7.6e-69
Identity = 127/339 (37.46%), Postives = 213/339 (62.83%), Query Frame = 0

Query: 3   SKLRKAIYAVKDQTSISLAKVSASGSGPNATSLEVTILKATTHDDVPLDHRYLAEILLFI 62
           SKL++AI AVKDQTS+ LAKV   G   + T LE+ ++KAT HDD P + +Y+ EIL   
Sbjct: 4   SKLKRAIGAVKDQTSVGLAKV--GGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCLT 63

Query: 63  SSNKSHAAVCVHSIAKRITRTRNWIVALKSLNLVFRIFQEGDPIFPREVLLAMKRGAKIL 122
           S ++++ + CV ++++R+ +T+NW VALK+L L+ R+  +GD  + +E+  A +RG ++L
Sbjct: 64  SYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRLL 123

Query: 123 NLSNFRDDSNSNPWDYTAFVRTFALYLDERLDCFLNGKLQKRYTQG-------------- 182
           N+S+FRD S S+ WDY+AFVRT+ALYLDERLD  + G+  K+ + G              
Sbjct: 124 NMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGRRGKKKSGGGGGGDGDSGEEDDH 183

Query: 183 ---------KTSRRMNEPIRDMKPTMLIDRISYWQKLLDRAIATRPTGSAKRNRLIQHSL 242
                    K     ++P+ +MK   + +R+ + Q+LLDR +A RPTG+AK NR++  ++
Sbjct: 184 RGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTGNAKNNRVVIVAM 243

Query: 243 HAVVQESFDLYRDISDGLALLLDSFFHLQYQSCVNAFHACVKAAKQFEELGSFYDVCKSI 302
           + +V+ESF LY +I++ + +L++ F  L     +  +    + +KQF+EL  FY  CK++
Sbjct: 244 YPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNM 303

Query: 303 GVGRTSEYPSVQQPSDELVATLQEFLKDHASFPCHGNRS 319
            V R+SEYP +++ + + +  + EF++D ++      +S
Sbjct: 304 AVARSSEYPELEKITQKKLDLMDEFIRDKSALAAQTTKS 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZVN67.5e-13046.48Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE... [more]
Q8S9J83.9e-7040.26Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8LF205.1e-7038.35Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX471.1e-6737.46Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SA653.4e-6638.96Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1JET60.0e+00100.00clathrin coat assembly protein AP180-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1ESQ21.2e-30391.85clathrin coat assembly protein AP180-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5D3C6851.4e-23273.46Clathrin coat assembly protein AP180 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BN171.4e-23273.46clathrin coat assembly protein AP180 OS=Cucumis melo OX=3656 GN=LOC103491352 PE=... [more]
A0A0A0KGJ53.6e-22872.29ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421580 PE=4 S... [more]
Match NameE-valueIdentityDescription
XP_022989087.10.0e+00100.00clathrin coat assembly protein AP180-like [Cucurbita maxima][more]
XP_023531390.11.4e-30692.07clathrin coat assembly protein AP180-like [Cucurbita pepo subsp. pepo][more]
XP_022930864.12.4e-30391.85clathrin coat assembly protein AP180-like [Cucurbita moschata][more]
KAG7022969.13.2e-30391.21Clathrin coat assembly protein, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG6589271.12.7e-30289.97Clathrin coat assembly protein 180, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT1G05020.15.3e-13146.48ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.12.8e-7140.26ENTH/ANTH/VHS superfamily protein [more]
AT4G32285.22.8e-7140.26ENTH/ANTH/VHS superfamily protein [more]
AT2G25430.13.6e-7138.35epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p... [more]
AT4G02650.17.6e-6937.46ENTH/ANTH/VHS superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 33..163
e-value: 3.1E-40
score: 149.7
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 27..163
score: 27.584972
IPR014712ANTH domain superfamilyGENE3D1.20.58.150ANTH domaincoord: 169..312
e-value: 3.4E-35
score: 122.8
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 35..307
e-value: 4.8E-83
score: 278.3
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 2..161
e-value: 4.2E-35
score: 122.6
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 33..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 343..362
NoneNo IPR availablePANTHERPTHR22951:SF75CLATHRIN COAT ASSEMBLY PROTEIN AP180coord: 1..563
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 1..563
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 35..156
e-value: 4.52797E-56
score: 182.438
NoneNo IPR availableSUPERFAMILY89009GAT-like domaincoord: 174..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G019310.1CmaCh11G019310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048268 clathrin coat assembly
biological_process GO:0072583 clathrin-dependent endocytosis
biological_process GO:0006900 vesicle budding from membrane
cellular_component GO:0005905 clathrin-coated pit
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005545 1-phosphatidylinositol binding
molecular_function GO:0032050 clathrin heavy chain binding
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0000149 SNARE binding
molecular_function GO:0030276 clathrin binding
molecular_function GO:0005543 phospholipid binding