CmaCh10G009960 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G009960
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionSNF1-related protein kinase regulatory subunit beta-1-like
LocationCma_Chr10: 5181466 .. 5185614 (-)
RNA-Seq ExpressionCmaCh10G009960
SyntenyCmaCh10G009960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAATGCCAATGGTAAGGAGGAGAGTTCTCCTGGCGGCGGTGGTGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGCCGGAGCAAGAGGTGACGGCGATGGACAAGGTCCGGCGATTGTCAGTTCTGTGACTAGTGAAACGAGTAACGCCATTTCTTCAGATTCGATGGGAAATACTCCTCCTGATAGCCCTGGGAAGTTCCGATCCCCGATCTTGTTCGCTCCTCAGGTCACTATTTCGTTCAATCTTTCTCCCTTGTGTGTACTGTGTTTGTATCTGTCTCTTCTTTCTGCTTTTCTTACTGTTGTTTTTGTTTGTTCGTGGGATTTGAATTATTCAATGATGTTGTTTTCATGAAAATACGTTTTATTGTTCAGTGGAAGTTGTGTTTCTCGGGAAATTTCTGGGAAAGTTCCAGCCAAGATCCACTTCGTTGACTGCTTGAACTAAGAATGACATCCAATAAGTTTAGTAATATTGAGTGTTCGTTATTATTGTTTTTTCACTTTGGATCATCTGCTTAGTGCTGCGTTCTTTGAAGGTTAATGGCTGAAATGTCTGTTACTGGATTTGCCTACTCTGATCGACTATAGTTTTATATGAACAGCTGAGATTTCTCTCTTAATCTTGGTCCCCTTCAAACTATTGATCAATTTAATAGGGTTGGTTGGTTGTTTGTTGTAATCAATTCATGGAGCAGATTGTTACTTCTTTAATGGTTAACTCATTGATAATACATTCATCGATTTCATAAGTGATGAGTTGTGATTAAACTTCTAGTTCCCGATTAGATTGAAATAGTTCGTAAGTTCGACTTCCTCGCTTTTGAGTCGATTCACATGTGAATTTGTGATTAACCTTGGTTCTGGGTTTTGAAATGATGGTGGGCATGTATTACAAGGCTTGCAGGTTCCTGTGGCTCCCTTAGGAGGTAATGGACCAACTCATTTCAATGAAACCTGGAAGAATGAACTCCATGAAGCTGTAGATAATCCTCCAGTGCAAGGAATTCCAACAATCATTACATGGAGCTACGGTGGCAGCAGCGTTGCTGTTGAGGGATCTTGGGACAACTGGGCGTCCAGGTTTATGATTTTTTCGAGCCTCATGCTTCATGACAGCAGTTCTACTGCATTTTGCATAAACAATGACATATAATGTAACATTGCTTTTGTAGGAAGACCCTACAGAGGTCTGGTAAAGATTTTTCAATACTTATGGTCCTTCCATCTGGAGTATATCATTACAAGTTCACCGTCGATGGTCAACGGAGATATATTCCGGATCTTCCTTTCATAGCTGATGAGATGGGCAATGTCTTTAATCTTCTTAATGTTTCTGTAAGTGTGATTCTTCCCTCCATCTGCATTACCATCCAATCGACATGAACTTGATGTTGACTCGTTATTTGTGTTGAAAAATTATGCGTTTATGGTCGTGTGAAAATGGAAATTACCACGTAGGTGCCAAAATACTTGCTTGATTCCATGTTAGGAGGATAAATATCTGAAGCGAGGCGATAGTTTAGCTCTGCTTCTCATGTTTATAATTTCAACCTTAAGAATGTGTGATAAATGCTAATGGTTGATTGTTTAAGCCCATTATTGGTCTTTTCACTCATATTCTGAGATCCCACATAGGTTGGGGAGGAGAATGAAGCATTCTTTATAAGGATGTGAAAACCTCTCCCTAGCAGACGCATTTTAAAAACCTTGAGTTGAAGCATGAAAGGAAAAGCTCAAAGAGGACAATATCTGCTAGTGGTAGGCTTGGGCTGTTATACATATGTTCAATATACATGTTTAGTGATATTATCGTCTTAAACTGGAAATGGACTAAAGAAAAGAATGAGGATAGCTCTCCACTCCCCAGGGCTGTAAAGTTATGTAGAAACCCTAGGCCTTATAAGTTCTAAAAGAAAAGAAGAAAAAAAGTGTGGCAGTGTTCTCACTAAGAGAGAAAGAAGAGAGAGTAAAGGAGTGAAGCAGCGCATGTGCTTACAAACCTGTTGGCTGGTCAGAATGATGCCAAAATCTTTTGGAACAAAGCCCTTTTTTCCTTTTTTGAACACTTCTCCTGAACACCAACGTGATTATCACAAGTTCTTTCTTTTCTTTTTTATTTTTAAGAAACGTGATTAGCACAGGTAATTGAGAGTTCTGTCTTGATCCTCTATCTATTAGCTGTAGACGTACCAAATACGATTCAGAAAGTATTTTCGTTATCATATCATGATTCATGTATGTGTTACTCTCCAAAGAACTACTCTTGCACCTGTTGATTGTTTTGGGGCACTTAATTTTATCATTTTTGGAGGAGAAGTACAAGTTTTAGCTTTATCGGGGAATTTTCCATCTCAGTAGGAGCTTTCCGGAACCCGGTTCGATAACGTTCTCTCATAGATTCACATTTTGATTAGAAAATTATAAGAAAAAGCAGAAAAGGTGCCTATACGATAGATTATTCTGGCTCTCTTTTCCTTTCTTCTAAATTTGTGCAACGAGACACATTCATACTGTTTGCACAGAAGACATTTCATGGTTCTTCTTGCTTGTCATGAGTGTTTACATTTTTCCAGTGGATTGTTCTATCGAAGTTCATTCAAAAGATACAGGAAGAGGAGAGAAATAATATGGACTAAAGTAGTTGTTCATGTGCCCAACCTAAATGAGCATTTGAGCTCTTAAAGGGGGGGAAATATATGTATACATTTACTCGCACATATGGTGTGAGATCCCACATCGGTTGGGGAGGAGAACGAAACATCCTTTATAAGAGTGTGAAAACCTCTCCCTAGCAGACGCATTGTAAAAACCTTGAGGGGAAGCCCGAAAGGGAAAATCCAAAGAGGCAGTATTTGCTAGCGGTGGGCTTGGGCCGTTACAAATGGTATCAGAGCTAGACTCTAGGCGATGTGCTAGCAAGGAGGCTAAGCTCCGAAGGGGGTAGACACGAGGCGGTGTGCCAGCAAGGATGCTGGGCCCAAAATGGAGTGGATTGGGGTACTACATCGATTGGAGAAGGGAATGAATGTCAGCAAGGAAGTTGGGCCCCAAAGGGGGGTGGATTGTGAGATCCCACATTGGTTGGGTAGGAGAACGAAATATCCTTTATAAGGGTGTGGAAACTTCTTCCTAGTATACGCGTTTTAAAAACCTTGAGGGGAAGCCCGAAAAGGAAAGCCTAAAGAGAACAATATCTGCTAGCGGTGGGTTTAGACCGTTACATACGCACACTTGCGCACGATAGGATAGGAGGTTGTAGCCTTCTCACATCTCTGGCATGTATTTATTATCTGAGCTCATATTCTACTGCGCCTGATAGTTTGCTGTATAAAATCCTGAATGCACATTGAGAAGTGTCCAAGATTTGTCAACATTGTTTAGAGAAAGATGCATTACTTCTTTAGTTAGTGCAATGCAAAAGACAAACTGATTTTATGCATTTCATCTTCACTCTTCATTTTGGAGTTGTTTCCATTCTGTAGGATAGTGTGCCTGATATTCTACAGAGCGTGGTCGAGTTCGAGGCTCCCCAATCACCAGAGACGACATACAGCCAGGCATTCCCCGGCGAGGAAGACTTCGCCAAGGAGCCAGCAGCCGTCCCATCACAGCTGCACCTCACAGTTCTAGGTATGGAGAACGCCGACGAAGCTTCGTCCTCAAAACCTCAACATGTTGTGCTTAACCACCTCTTCATAGAGAAAGGATGGGCTTCTCAGTCTGTGGTTGCCTTGGGACTTACTCATAGGTTCCATTCAAAGTATGTCACTGTCGTCCTCTTCAAACCTCTCAACAGGTAGTTATCGTCCATTTTTCGCTCTGGCTGAACTTTGTAAAGGATCACGAGCTACTTCTCGCTCCATCATCCATGGAGGGAATCTTGCTTTATAGAACTGTGTTGGCCTAAGTTTGCTTACAACAAAAAACTCTTATTCTGATCAATATTTCTTCAATTTGTGAAGAAATCTTTGTTTTTATAGAACTTGTAGGCCAAAAAAGTTTCATTACCTTTTTCAAAATGTATCAAAAGTAGTAGATTTTGATAGCATATTTGTCTGCCTCAGTTTTAGCATTCTATTCCTCAGTGTGCTACGTTTGACAAATTAATTTGGTGCCTGATTTTAGATGGATTGTTAAATAA

mRNA sequence

ATGGGAAATGCCAATGGTAAGGAGGAGAGTTCTCCTGGCGGCGGTGGTGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGCCGGAGCAAGAGGTGACGGCGATGGACAAGGTCCGGCGATTGTCAGTTCTGTGACTAGTGAAACGAGTAACGCCATTTCTTCAGATTCGATGGGAAATACTCCTCCTGATAGCCCTGGGAAGTTCCGATCCCCGATCTTGTTCGCTCCTCAGGTTCCTGTGGCTCCCTTAGGAGGTAATGGACCAACTCATTTCAATGAAACCTGGAAGAATGAACTCCATGAAGCTGTAGATAATCCTCCAGTGCAAGGAATTCCAACAATCATTACATGGAGCTACGGTGGCAGCAGCGTTGCTGTTGAGGGATCTTGGGACAACTGGGCGTCCAGGAAGACCCTACAGAGGTCTGGTAAAGATTTTTCAATACTTATGGTCCTTCCATCTGGAGTATATCATTACAAGTTCACCGTCGATGGTCAACGGAGATATATTCCGGATCTTCCTTTCATAGCTGATGAGATGGGCAATGTCTTTAATCTTCTTAATGTTTCTGATAGTGTGCCTGATATTCTACAGAGCGTGGTCGAGTTCGAGGCTCCCCAATCACCAGAGACGACATACAGCCAGGCATTCCCCGGCGAGGAAGACTTCGCCAAGGAGCCAGCAGCCGTCCCATCACAGCTGCACCTCACAGTTCTAGGTATGGAGAACGCCGACGAAGCTTCGTCCTCAAAACCTCAACATGTTGTGCTTAACCACCTCTTCATAGAGAAAGGATGGGCTTCTCAGTCTGTGGTTGCCTTGGGACTTACTCATAGGTTCCATTCAAAGTATGTCACTGTCGTCCTCTTCAAACCTCTCAACAGATGGATTGTTAAATAA

Coding sequence (CDS)

ATGGGAAATGCCAATGGTAAGGAGGAGAGTTCTCCTGGCGGCGGTGGTGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGCCGGAGCAAGAGGTGACGGCGATGGACAAGGTCCGGCGATTGTCAGTTCTGTGACTAGTGAAACGAGTAACGCCATTTCTTCAGATTCGATGGGAAATACTCCTCCTGATAGCCCTGGGAAGTTCCGATCCCCGATCTTGTTCGCTCCTCAGGTTCCTGTGGCTCCCTTAGGAGGTAATGGACCAACTCATTTCAATGAAACCTGGAAGAATGAACTCCATGAAGCTGTAGATAATCCTCCAGTGCAAGGAATTCCAACAATCATTACATGGAGCTACGGTGGCAGCAGCGTTGCTGTTGAGGGATCTTGGGACAACTGGGCGTCCAGGAAGACCCTACAGAGGTCTGGTAAAGATTTTTCAATACTTATGGTCCTTCCATCTGGAGTATATCATTACAAGTTCACCGTCGATGGTCAACGGAGATATATTCCGGATCTTCCTTTCATAGCTGATGAGATGGGCAATGTCTTTAATCTTCTTAATGTTTCTGATAGTGTGCCTGATATTCTACAGAGCGTGGTCGAGTTCGAGGCTCCCCAATCACCAGAGACGACATACAGCCAGGCATTCCCCGGCGAGGAAGACTTCGCCAAGGAGCCAGCAGCCGTCCCATCACAGCTGCACCTCACAGTTCTAGGTATGGAGAACGCCGACGAAGCTTCGTCCTCAAAACCTCAACATGTTGTGCTTAACCACCTCTTCATAGAGAAAGGATGGGCTTCTCAGTCTGTGGTTGCCTTGGGACTTACTCATAGGTTCCATTCAAAGTATGTCACTGTCGTCCTCTTCAAACCTCTCAACAGATGGATTGTTAAATAA

Protein sequence

MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNTPPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYGGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEMGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLGMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNRWIVK
Homology
BLAST of CmaCh10G009960 vs. ExPASy Swiss-Prot
Match: Q84VQ1 (SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX=3702 GN=KINB1 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 9.6e-84
Identity = 173/296 (58.45%), Postives = 207/296 (69.93%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGK+E +      G GG     + AR +G                   SSDSM ++
Sbjct: 1   MGNANGKDEDA----AAGSGGADVTSSSARSNGGDPS-------ARSRHRRPSSDSMSSS 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPL-GGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSY 120
           PP SP +  SP LFAPQVPVAPL   N P   N  W N+     DNPP QGIPTIITW+ 
Sbjct: 61  PPGSPARSPSPFLFAPQVPVAPLQRANAPPPNNIQW-NQSQRVFDNPPEQGIPTIITWNQ 120

Query: 121 GGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADE 180
           GG+ VAVEGSWDNW SRK LQ+SGKD SIL VLPSG+YHYK  VDG+ +YIPDLPF+ADE
Sbjct: 121 GGNDVAVEGSWDNWRSRKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADE 180

Query: 181 MGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVL 240
           +GNV N+L+V + VP+  +S+VEFEAP SP+ +Y Q  P  ED+AKEP AVP QLHLT+L
Sbjct: 181 VGNVCNILDVHNFVPENPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLL 240

Query: 241 GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           G    + A ++KPQHVVLNH+FIE+GW  QS+VALGLTHRF SKY+TVVL+KPL R
Sbjct: 241 G-TTEETAIATKPQHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLTR 283

BLAST of CmaCh10G009960 vs. ExPASy Swiss-Prot
Match: Q9SCY5 (SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX=3702 GN=KINB2 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.1e-66
Identity = 144/303 (47.52%), Postives = 190/303 (62.71%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQ-GPAIVSSVTSETSNAISSDSMGN 60
           MGN N +EE++               A A  D D +       S  S+ ++    + MG 
Sbjct: 1   MGNVNAREEAN------------SNNASAVEDEDAEICSREAMSAASDGNHVAPPELMGQ 60

Query: 61  TPPDSPGKFRSPILFAPQVPVAPLGGNGPTHF-NETW---KNELHEAVDNPPVQGIPTII 120
           +PP SP   +SP++FAPQVPV PL      H  N +W    + L+E   N   QGIPT+I
Sbjct: 61  SPPHSPRATQSPLMFAPQVPVLPLQRPDEIHIPNPSWMQSPSSLYEEASNE--QGIPTMI 120

Query: 121 TWSYGGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPF 180
           TW +GG  +AVEGSWDNW +R  LQRSGKDF+I+ VLPSGVY Y+F VDGQ R+ P+LP 
Sbjct: 121 TWCHGGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPL 180

Query: 181 IADEMGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLH 240
             D+ GN FN+L++ D VP+ +QS+  FE PQSPE +YS    G ED++KEP  VP  L 
Sbjct: 181 ARDDAGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQ 240

Query: 241 LTVLGMENADEASSS---KPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKP 296
           +T+L +  A+    S   +PQHV+LNHL+++KG +  SVVALG THRF +KYVTVVL+K 
Sbjct: 241 MTLLNLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKS 289

BLAST of CmaCh10G009960 vs. ExPASy Swiss-Prot
Match: O43741 (5'-AMP-activated protein kinase subunit beta-2 OS=Homo sapiens OX=9606 GN=PRKAB2 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.4e-28
Identity = 75/217 (34.56%), Postives = 119/217 (54.84%), Query Frame = 0

Query: 94  TWKNELHEAVDNPPVQGIPTIITWSYGGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLP 153
           +W+ +L ++V  P  Q  PT+I WS GG  V + GS++NW+++  L +S  DF  ++ LP
Sbjct: 61  SWQQDLEDSV-KPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLP 120

Query: 154 SGVYHYKFTVDGQRRYIPDLPFIADEMGNVFNLLNVSDSVPDILQSVVEFEAPQSPETT- 213
            G + YKF VDGQ  + P  P +  ++G + NL++V  S  ++  + ++ ++ +S ET+ 
Sbjct: 121 EGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDA-LKLDSMESSETSC 180

Query: 214 ----------YSQ---AFPGEEDFAKEPAAVPSQLHLTVLGME---NADEASSSKPQHVV 273
                     Y Q   AF  EE F K P  +P  L   +L  +   + D A   +P HV+
Sbjct: 181 RDLSSSPPGPYGQEMYAFRSEERF-KSPPILPPHLLQVILNKDTNISCDPALLPEPNHVM 240

Query: 274 LNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPL 294
           LNHL+        SV+ L  THR+  KYVT +L+KP+
Sbjct: 241 LNHLYALS--IKDSVMVLSATHRYKKKYVTTLLYKPI 272

BLAST of CmaCh10G009960 vs. ExPASy Swiss-Prot
Match: Q9QZH4 (5'-AMP-activated protein kinase subunit beta-2 OS=Rattus norvegicus OX=10116 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.2e-25
Identity = 90/286 (31.47%), Postives = 137/286 (47.90%), Query Frame = 0

Query: 25  GGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNTPPDSPGKFRSPILFAPQVPVAPLG 84
           G   AR +G G GP     +            +G+T  D P  F  P    P        
Sbjct: 15  GAKAARAEGGGHGPGKEHKI-----------MVGST--DDPSVFSLPDSKLP-------- 74

Query: 85  GNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYGGSSVAVEGSWDNWASRKTLQRSGK 144
             G   F   W+ +L ++V  P  Q  PT+I WS GG  V + GS++NW+++  L +S  
Sbjct: 75  --GDKEF-VPWQQDLDDSV-KPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHN 134

Query: 145 DFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEMGNVFNLLNVSDSVPDILQSVVEFE 204
           DF  ++ LP G + YKF VDGQ  + P  P +  ++G + NL++V  S  ++  + ++ +
Sbjct: 135 DFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDA-LKLD 194

Query: 205 APQSPETT-----------YSQ---AFPGEEDFAKEPAAVPSQLHLTVLGME---NADEA 264
           + +S ET+           Y Q    F  EE F K P  +P  L   +L  +   + D A
Sbjct: 195 SMESSETSCRDLSSSPPGPYGQEMYVFRSEERF-KSPPILPPHLLQVILNKDTNISCDPA 254

Query: 265 SSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPL 294
              +P HV+LNHL+        SV+ L  THR+  KYVT +L+KP+
Sbjct: 255 LLPEPNHVMLNHLYALS--TKDSVMVLSATHRYKKKYVTTLLYKPI 271

BLAST of CmaCh10G009960 vs. ExPASy Swiss-Prot
Match: Q6PAM0 (5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-25
Identity = 90/286 (31.47%), Postives = 137/286 (47.90%), Query Frame = 0

Query: 25  GGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNTPPDSPGKFRSPILFAPQVPVAPLG 84
           G   AR +G G GP     +            +G+T  D P  F  P    P        
Sbjct: 15  GAKAARAEGGGHGPGKEHKI-----------MVGST--DDPSVFSLPDSKLP-------- 74

Query: 85  GNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYGGSSVAVEGSWDNWASRKTLQRSGK 144
             G   F   W+ +L ++V  P  Q  PT+I WS GG  V + GS++NW+++  L +S  
Sbjct: 75  --GDKEF-VPWQQDLDDSV-KPAQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHN 134

Query: 145 DFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEMGNVFNLLNVSDSVPDILQSVVEFE 204
           DF  ++ LP G + YKF VDGQ  + P  P +  ++G + NL++V  S  ++  + ++ +
Sbjct: 135 DFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDA-LKLD 194

Query: 205 APQSPETT-----------YSQ---AFPGEEDFAKEPAAVPSQLHLTVLGME---NADEA 264
           + +S ET+           Y Q    F  EE F K P  +P  L   +L  +   + D A
Sbjct: 195 SMESSETSCRDLSSSPPGPYGQEMYVFRSEERF-KSPPILPPHLLQVILNKDTNISCDPA 254

Query: 265 SSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPL 294
              +P HV+LNHL+        SV+ L  THR+  KYVT +L+KP+
Sbjct: 255 LLPEPNHVMLNHLYALS--IKDSVMVLSATHRYKKKYVTTLLYKPI 271

BLAST of CmaCh10G009960 vs. ExPASy TrEMBL
Match: A0A6J1JIV6 (SNF1-related protein kinase regulatory subunit beta-1-like OS=Cucurbita maxima OX=3661 GN=LOC111485497 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 7.7e-161
Identity = 295/295 (100.00%), Postives = 295/295 (100.00%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT
Sbjct: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG 120
           PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG
Sbjct: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG 120

Query: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180
           GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM
Sbjct: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180

Query: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240
           GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG
Sbjct: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240

Query: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR
Sbjct: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 295

BLAST of CmaCh10G009960 vs. ExPASy TrEMBL
Match: A0A6J1H851 (SNF1-related protein kinase regulatory subunit beta-1-like OS=Cucurbita moschata OX=3662 GN=LOC111461411 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.6e-153
Identity = 284/295 (96.27%), Postives = 285/295 (96.61%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGKEESSPGGG GGGGG GG     RGD D QGPAIVSSVTSETSNAISSDSMGNT
Sbjct: 1   MGNANGKEESSPGGGSGGGGGAGG-----RGDADRQGPAIVSSVTSETSNAISSDSMGNT 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG 120
           PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNE W+NELHEAVDNPPVQGIPTIITWSYG
Sbjct: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNEAWQNELHEAVDNPPVQGIPTIITWSYG 120

Query: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180
           GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM
Sbjct: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180

Query: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240
           GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG
Sbjct: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240

Query: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR
Sbjct: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 290

BLAST of CmaCh10G009960 vs. ExPASy TrEMBL
Match: A0A6J1F546 (SNF1-related protein kinase regulatory subunit beta-1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111440954 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 8.6e-136
Identity = 250/296 (84.46%), Postives = 262/296 (88.51%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANG+EESSPGGGG           GA  D DGQG  I SS+ SETSN +SSDSM NT
Sbjct: 1   MGNANGREESSPGGGG-----------GAVADADGQGSVIDSSLVSETSNVVSSDSMVNT 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPL-GGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSY 120
           PP SPG  RSPILFAPQVPVAPL GGNGPTH+NE W+NE HE VDNPP QGIPTIITWSY
Sbjct: 61  PPGSPGNLRSPILFAPQVPVAPLQGGNGPTHYNEAWQNEFHETVDNPPEQGIPTIITWSY 120

Query: 121 GGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADE 180
           GGSSVA+EGSWDNWASRKTLQR+GKDFSIL+VLPSGVYHYKF VDGQ+RYIPDLP+IADE
Sbjct: 121 GGSSVAIEGSWDNWASRKTLQRTGKDFSILIVLPSGVYHYKFIVDGQKRYIPDLPYIADE 180

Query: 181 MGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVL 240
           MGNVFNLLNVSDS PDILQS+ EFEAP SPETTYSQ FP EEDFAKEPAAVPSQLHLTVL
Sbjct: 181 MGNVFNLLNVSDSAPDILQSLAEFEAPPSPETTYSQVFPSEEDFAKEPAAVPSQLHLTVL 240

Query: 241 GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVL+KPLNR
Sbjct: 241 GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLYKPLNR 285

BLAST of CmaCh10G009960 vs. ExPASy TrEMBL
Match: A0A6J1HWQ6 (SNF1-related protein kinase regulatory subunit beta-1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467354 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.8e-134
Identity = 248/296 (83.78%), Postives = 259/296 (87.50%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANG+EESSPGGGG           GA  D  GQGP I SS+ SETSN +SSDSM NT
Sbjct: 1   MGNANGREESSPGGGG-----------GAVADAGGQGPVIGSSLVSETSNVVSSDSMVNT 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPL-GGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSY 120
           PP SPG  RSPILFAPQVPVAPL GGNGPTH+NE W+NE HE VDNPP QGIPTIITWSY
Sbjct: 61  PPGSPGNLRSPILFAPQVPVAPLQGGNGPTHYNEAWQNEFHETVDNPPEQGIPTIITWSY 120

Query: 121 GGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADE 180
           GG SVA+EGSWDNWASRK LQR+GKDFSIL+VLPSGVYHYKF VDGQ+RYIPDLP IADE
Sbjct: 121 GGGSVAIEGSWDNWASRKILQRTGKDFSILIVLPSGVYHYKFIVDGQKRYIPDLPHIADE 180

Query: 181 MGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVL 240
           MGNVFNLLNVSDS PDILQS+ EFEAP SPETTYSQ FP EEDFAKEPAAVPSQLHLTVL
Sbjct: 181 MGNVFNLLNVSDSAPDILQSLAEFEAPPSPETTYSQVFPSEEDFAKEPAAVPSQLHLTVL 240

Query: 241 GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVL+KPLNR
Sbjct: 241 GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLYKPLNR 285

BLAST of CmaCh10G009960 vs. ExPASy TrEMBL
Match: A0A5D3CDJ6 (SNF1-related protein kinase regulatory subunit beta-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00700 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.5e-132
Identity = 249/296 (84.12%), Postives = 262/296 (88.51%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANG+E+S+PG    GG          R D D Q PAI SSV SETSN +SSDSMGNT
Sbjct: 1   MGNANGREQSTPGAPAAGG----------RADVDAQSPAI-SSVNSETSNVVSSDSMGNT 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPL-GGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSY 120
           PP SPGKFRSPILFAPQ+PVAPL GGNGPTH+N  W+NE   A+D+PP QGIPTIITWSY
Sbjct: 61  PPHSPGKFRSPILFAPQIPVAPLQGGNGPTHYNGAWQNEFDGAIDSPPEQGIPTIITWSY 120

Query: 121 GGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADE 180
           GGS+VAVEGSWDNWASRKTLQR+GKDFS+LMVLPSGVYHYKF VDGQRRYIPDLPFIADE
Sbjct: 121 GGSNVAVEGSWDNWASRKTLQRTGKDFSLLMVLPSGVYHYKFIVDGQRRYIPDLPFIADE 180

Query: 181 MGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVL 240
           MGNVFNLLNVSDSVPDILQSV EFE PQSPETTYSQ FP EEDFAKEPAAVPSQLHLTVL
Sbjct: 181 MGNVFNLLNVSDSVPDILQSVAEFEPPQSPETTYSQTFPTEEDFAKEPAAVPSQLHLTVL 240

Query: 241 GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVL+KPLNR
Sbjct: 241 GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLYKPLNR 285

BLAST of CmaCh10G009960 vs. NCBI nr
Match: XP_022988180.1 (SNF1-related protein kinase regulatory subunit beta-1-like [Cucurbita maxima])

HSP 1 Score: 576.2 bits (1484), Expect = 1.6e-160
Identity = 295/295 (100.00%), Postives = 295/295 (100.00%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT
Sbjct: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG 120
           PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG
Sbjct: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG 120

Query: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180
           GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM
Sbjct: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180

Query: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240
           GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG
Sbjct: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240

Query: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR
Sbjct: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 295

BLAST of CmaCh10G009960 vs. NCBI nr
Match: XP_023516552.1 (SNF1-related protein kinase regulatory subunit beta-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558.1 bits (1437), Expect = 4.5e-155
Identity = 287/295 (97.29%), Postives = 289/295 (97.97%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGKEESSPGGGGGGGGGG GG    RGDGDGQGPAIVSSVTSETSNAISSDSMGNT
Sbjct: 1   MGNANGKEESSPGGGGGGGGGGAGG----RGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG 120
           PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNE W+NELHEAVDNPPVQGIPTIITWSYG
Sbjct: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNEAWQNELHEAVDNPPVQGIPTIITWSYG 120

Query: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180
           GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM
Sbjct: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180

Query: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240
           GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG
Sbjct: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240

Query: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           ME+ADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR
Sbjct: 241 MEDADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 291

BLAST of CmaCh10G009960 vs. NCBI nr
Match: KAG7033451.1 (SNF1-related protein kinase regulatory subunit beta-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 557.0 bits (1434), Expect = 1.0e-154
Identity = 287/295 (97.29%), Postives = 288/295 (97.63%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGKEESSPGGGGGGGGGG GG    RGDGD QGPAIVSSVTSETSNAISSDSMGNT
Sbjct: 1   MGNANGKEESSPGGGGGGGGGGAGG----RGDGDRQGPAIVSSVTSETSNAISSDSMGNT 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG 120
           PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNE W+NELHEAVDNPPVQGIPTIITWSYG
Sbjct: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNEAWQNELHEAVDNPPVQGIPTIITWSYG 120

Query: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180
           GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM
Sbjct: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180

Query: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240
           GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG
Sbjct: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240

Query: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR
Sbjct: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 291

BLAST of CmaCh10G009960 vs. NCBI nr
Match: KAG6590266.1 (BTB/POZ and MATH domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 555.1 bits (1429), Expect = 3.8e-154
Identity = 285/295 (96.61%), Postives = 287/295 (97.29%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGKEESSPGGGGGGGGGG GG    RGDGD QGPAIVSSVTSETSNAISSDSMGNT
Sbjct: 426 MGNANGKEESSPGGGGGGGGGGAGG----RGDGDRQGPAIVSSVTSETSNAISSDSMGNT 485

Query: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG 120
           PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNE W+NELHEAVDNPPVQGIPTIITWSYG
Sbjct: 486 PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNEAWQNELHEAVDNPPVQGIPTIITWSYG 545

Query: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180
           GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM
Sbjct: 546 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 605

Query: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240
           GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQ FPGEEDFAKEPAA+PSQLHLTVLG
Sbjct: 606 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQGFPGEEDFAKEPAAIPSQLHLTVLG 665

Query: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR
Sbjct: 666 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 716

BLAST of CmaCh10G009960 vs. NCBI nr
Match: XP_022960692.1 (SNF1-related protein kinase regulatory subunit beta-1-like [Cucurbita moschata])

HSP 1 Score: 551.2 bits (1419), Expect = 5.5e-153
Identity = 284/295 (96.27%), Postives = 285/295 (96.61%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGKEESSPGGG GGGGG GG     RGD D QGPAIVSSVTSETSNAISSDSMGNT
Sbjct: 1   MGNANGKEESSPGGGSGGGGGAGG-----RGDADRQGPAIVSSVTSETSNAISSDSMGNT 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYG 120
           PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNE W+NELHEAVDNPPVQGIPTIITWSYG
Sbjct: 61  PPDSPGKFRSPILFAPQVPVAPLGGNGPTHFNEAWQNELHEAVDNPPVQGIPTIITWSYG 120

Query: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180
           GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM
Sbjct: 121 GSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEM 180

Query: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240
           GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG
Sbjct: 181 GNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLG 240

Query: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR
Sbjct: 241 MENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 290

BLAST of CmaCh10G009960 vs. TAIR 10
Match: AT5G21170.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 311.6 bits (797), Expect = 6.8e-85
Identity = 173/296 (58.45%), Postives = 207/296 (69.93%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGK+E +      G GG     + AR +G                   SSDSM ++
Sbjct: 1   MGNANGKDEDA----AAGSGGADVTSSSARSNGGDPS-------ARSRHRRPSSDSMSSS 60

Query: 61  PPDSPGKFRSPILFAPQVPVAPL-GGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSY 120
           PP SP +  SP LFAPQVPVAPL   N P   N  W N+     DNPP QGIPTIITW+ 
Sbjct: 61  PPGSPARSPSPFLFAPQVPVAPLQRANAPPPNNIQW-NQSQRVFDNPPEQGIPTIITWNQ 120

Query: 121 GGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADE 180
           GG+ VAVEGSWDNW SRK LQ+SGKD SIL VLPSG+YHYK  VDG+ +YIPDLPF+ADE
Sbjct: 121 GGNDVAVEGSWDNWRSRKKLQKSGKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADE 180

Query: 181 MGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVL 240
           +GNV N+L+V + VP+  +S+VEFEAP SP+ +Y Q  P  ED+AKEP AVP QLHLT+L
Sbjct: 181 VGNVCNILDVHNFVPENPESIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLL 240

Query: 241 GMENADEASSSKPQHVVLNHLFIEKGWASQSVVALGLTHRFHSKYVTVVLFKPLNR 296
           G    + A ++KPQHVVLNH+FIE+GW  QS+VALGLTHRF SKY+TVVL+KPL R
Sbjct: 241 G-TTEETAIATKPQHVVLNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLTR 283

BLAST of CmaCh10G009960 vs. TAIR 10
Match: AT5G21170.2 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 293.1 bits (749), Expect = 2.5e-79
Identity = 173/333 (51.95%), Postives = 207/333 (62.16%), Query Frame = 0

Query: 1   MGNANGKEESSPGGGGGGGGGGGGGGAGARGDGDGQGPAIVSSVTSETSNAISSDSMGNT 60
           MGNANGK+E +      G GG     + AR +G                   SSDSM ++
Sbjct: 1   MGNANGKDEDA----AAGSGGADVTSSSARSNGGDPS-------ARSRHRRPSSDSMSSS 60

Query: 61  PPDSPGKFRSPILFAPQ-------------------------------------VPVAPL 120
           PP SP +  SP LFAPQ                                     VPVAPL
Sbjct: 61  PPGSPARSPSPFLFAPQPTLLFHVTSYNICSPMRHLFHCLGLTCVPNGCISCRMVPVAPL 120

Query: 121 -GGNGPTHFNETWKNELHEAVDNPPVQGIPTIITWSYGGSSVAVEGSWDNWASRKTLQRS 180
              N P   N  W N+     DNPP QGIPTIITW+ GG+ VAVEGSWDNW SRK LQ+S
Sbjct: 121 QRANAPPPNNIQW-NQSQRVFDNPPEQGIPTIITWNQGGNDVAVEGSWDNWRSRKKLQKS 180

Query: 181 GKDFSILMVLPSGVYHYKFTVDGQRRYIPDLPFIADEMGNVFNLLNVSDSVPDILQSVVE 240
           GKD SIL VLPSG+YHYK  VDG+ +YIPDLPF+ADE+GNV N+L+V + VP+  +S+VE
Sbjct: 181 GKDHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADEVGNVCNILDVHNFVPENPESIVE 240

Query: 241 FEAPQSPETTYSQAFPGEEDFAKEPAAVPSQLHLTVLGMENADEASSSKPQHVVLNHLFI 296
           FEAP SP+ +Y Q  P  ED+AKEP AVP QLHLT+LG    + A ++KPQHVVLNH+FI
Sbjct: 241 FEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLLG-TTEETAIATKPQHVVLNHVFI 300

BLAST of CmaCh10G009960 vs. TAIR 10
Match: AT4G16360.1 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 265.0 bits (676), Expect = 7.3e-71
Identity = 134/260 (51.54%), Postives = 176/260 (67.69%), Query Frame = 0

Query: 43  SVTSETSNAISSDSMGNTPPDSPGKFRSPILFAPQVPVAPLGGNGPTHF-NETW---KNE 102
           S  S+ ++    + MG +PP SP   +SP++FAPQVPV PL      H  N +W    + 
Sbjct: 2   SAASDGNHVAPPELMGQSPPHSPRATQSPLMFAPQVPVLPLQRPDEIHIPNPSWMQSPSS 61

Query: 103 LHEAVDNPPVQGIPTIITWSYGGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYH 162
           L+E   N   QGIPT+ITW +GG  +AVEGSWDNW +R  LQRSGKDF+I+ VLPSGVY 
Sbjct: 62  LYEEASNE--QGIPTMITWCHGGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYE 121

Query: 163 YKFTVDGQRRYIPDLPFIADEMGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFP 222
           Y+F VDGQ R+ P+LP   D+ GN FN+L++ D VP+ +QS+  FE PQSPE +YS    
Sbjct: 122 YRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLL 181

Query: 223 GEEDFAKEPAAVPSQLHLTVLGMENADEASSS---KPQHVVLNHLFIEKGWASQSVVALG 282
           G ED++KEP  VP  L +T+L +  A+    S   +PQHV+LNHL+++KG +  SVVALG
Sbjct: 182 GAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALG 241

Query: 283 LTHRFHSKYVTVVLFKPLNR 296
            THRF +KYVTVVL+K L R
Sbjct: 242 STHRFLAKYVTVVLYKSLQR 259

BLAST of CmaCh10G009960 vs. TAIR 10
Match: AT4G16360.3 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 265.0 bits (676), Expect = 7.3e-71
Identity = 134/260 (51.54%), Postives = 176/260 (67.69%), Query Frame = 0

Query: 43  SVTSETSNAISSDSMGNTPPDSPGKFRSPILFAPQVPVAPLGGNGPTHF-NETW---KNE 102
           S  S+ ++    + MG +PP SP   +SP++FAPQVPV PL      H  N +W    + 
Sbjct: 4   SAASDGNHVAPPELMGQSPPHSPRATQSPLMFAPQVPVLPLQRPDEIHIPNPSWMQSPSS 63

Query: 103 LHEAVDNPPVQGIPTIITWSYGGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYH 162
           L+E   N   QGIPT+ITW +GG  +AVEGSWDNW +R  LQRSGKDF+I+ VLPSGVY 
Sbjct: 64  LYEEASNE--QGIPTMITWCHGGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYE 123

Query: 163 YKFTVDGQRRYIPDLPFIADEMGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFP 222
           Y+F VDGQ R+ P+LP   D+ GN FN+L++ D VP+ +QS+  FE PQSPE +YS    
Sbjct: 124 YRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLL 183

Query: 223 GEEDFAKEPAAVPSQLHLTVLGMENADEASSS---KPQHVVLNHLFIEKGWASQSVVALG 282
           G ED++KEP  VP  L +T+L +  A+    S   +PQHV+LNHL+++KG +  SVVALG
Sbjct: 184 GAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALG 243

Query: 283 LTHRFHSKYVTVVLFKPLNR 296
            THRF +KYVTVVL+K L R
Sbjct: 244 STHRFLAKYVTVVLYKSLQR 261

BLAST of CmaCh10G009960 vs. TAIR 10
Match: AT4G16360.2 (5'-AMP-activated protein kinase beta-2 subunit protein )

HSP 1 Score: 259.2 bits (661), Expect = 4.0e-69
Identity = 133/260 (51.15%), Postives = 176/260 (67.69%), Query Frame = 0

Query: 43  SVTSETSNAISSDSMGNTPPDSPGKFRSPILFAPQVPVAPLGGNGPTHF-NETW---KNE 102
           S  S+ ++    + MG +PP SP   +SP++FAPQVPV PL      H  N +W    + 
Sbjct: 2   SAASDGNHVAPPELMGQSPPHSPRATQSPLMFAPQVPVLPLQRPDEIHIPNPSWMQSPSS 61

Query: 103 LHEAVDNPPVQGIPTIITWSYGGSSVAVEGSWDNWASRKTLQRSGKDFSILMVLPSGVYH 162
           L+E   N   QGIPT+ITW +GG  +AVEGSWDNW + + LQRSGKDF+I+ VLPSGVY 
Sbjct: 62  LYEEASNE--QGIPTMITWCHGGKEIAVEGSWDNWKTSR-LQRSGKDFTIMKVLPSGVYE 121

Query: 163 YKFTVDGQRRYIPDLPFIADEMGNVFNLLNVSDSVPDILQSVVEFEAPQSPETTYSQAFP 222
           Y+F VDGQ R+ P+LP   D+ GN FN+L++ D VP+ +QS+  FE PQSPE +YS    
Sbjct: 122 YRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLL 181

Query: 223 GEEDFAKEPAAVPSQLHLTVLGMENADEASSS---KPQHVVLNHLFIEKGWASQSVVALG 282
           G ED++KEP  VP  L +T+L +  A+    S   +PQHV+LNHL+++KG +  SVVALG
Sbjct: 182 GAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALG 241

Query: 283 LTHRFHSKYVTVVLFKPLNR 296
            THRF +KYVTVVL+K L R
Sbjct: 242 STHRFLAKYVTVVLYKSLQR 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84VQ19.6e-8458.45SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX... [more]
Q9SCY51.1e-6647.52SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX... [more]
O437414.4e-2834.565'-AMP-activated protein kinase subunit beta-2 OS=Homo sapiens OX=9606 GN=PRKAB2... [more]
Q9QZH41.2e-2531.475'-AMP-activated protein kinase subunit beta-2 OS=Rattus norvegicus OX=10116 GN=... [more]
Q6PAM01.6e-2531.475'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab... [more]
Match NameE-valueIdentityDescription
A0A6J1JIV67.7e-161100.00SNF1-related protein kinase regulatory subunit beta-1-like OS=Cucurbita maxima O... [more]
A0A6J1H8512.6e-15396.27SNF1-related protein kinase regulatory subunit beta-1-like OS=Cucurbita moschata... [more]
A0A6J1F5468.6e-13684.46SNF1-related protein kinase regulatory subunit beta-1 isoform X1 OS=Cucurbita mo... [more]
A0A6J1HWQ62.8e-13483.78SNF1-related protein kinase regulatory subunit beta-1 isoform X1 OS=Cucurbita ma... [more]
A0A5D3CDJ61.5e-13284.12SNF1-related protein kinase regulatory subunit beta-1 OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
XP_022988180.11.6e-160100.00SNF1-related protein kinase regulatory subunit beta-1-like [Cucurbita maxima][more]
XP_023516552.14.5e-15597.29SNF1-related protein kinase regulatory subunit beta-1-like [Cucurbita pepo subsp... [more]
KAG7033451.11.0e-15497.29SNF1-related protein kinase regulatory subunit beta-1 [Cucurbita argyrosperma su... [more]
KAG6590266.13.8e-15496.61BTB/POZ and MATH domain-containing protein 4, partial [Cucurbita argyrosperma su... [more]
XP_022960692.15.5e-15396.27SNF1-related protein kinase regulatory subunit beta-1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G21170.16.8e-8558.455'-AMP-activated protein kinase beta-2 subunit protein [more]
AT5G21170.22.5e-7951.955'-AMP-activated protein kinase beta-2 subunit protein [more]
AT4G16360.17.3e-7151.545'-AMP-activated protein kinase beta-2 subunit protein [more]
AT4G16360.37.3e-7151.545'-AMP-activated protein kinase beta-2 subunit protein [more]
AT4G16360.24.0e-6951.155'-AMP-activated protein kinase beta-2 subunit protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006828Association with the SNF1 complex (ASC) domainSMARTSM01010AMPKBI_2coord: 206..293
e-value: 2.0E-21
score: 87.2
IPR006828Association with the SNF1 complex (ASC) domainPFAMPF04739AMPKBIcoord: 224..292
e-value: 1.5E-17
score: 63.6
IPR032640AMP-activated protein kinase, glycogen-binding domainPFAMPF16561AMPK1_CBMcoord: 111..192
e-value: 6.0E-30
score: 103.3
NoneNo IPR availableGENE3D6.20.250.60coord: 199..293
e-value: 2.3E-20
score: 74.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..63
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..71
NoneNo IPR availableCDDcd02859E_set_AMPKbeta_like_Ncoord: 112..189
e-value: 1.10256E-23
score: 90.3508
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 105..195
e-value: 1.3E-21
score: 78.2
IPR043554SNF1-related protein kinase regulatory subunit betaPANTHERPTHR46316SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1coord: 1..295
IPR037170SNF1-related protein kinase regulatory subunit beta-1PANTHERPTHR46316:SF9SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1coord: 1..295
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 110..190
IPR037256ASC domain superfamilySUPERFAMILY160219AMPKBI-likecoord: 193..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G009960.1CmaCh10G009960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043562 cellular response to nitrogen levels
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0009507 chloroplast
cellular_component GO:0031588 nucleotide-activated protein kinase complex
molecular_function GO:1901982 maltose binding
molecular_function GO:0005515 protein binding
molecular_function GO:0019887 protein kinase regulator activity