CmaCh10G009000 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh10G009000 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.4e-159
Identity = 278/520 (53.46%), Postives = 372/520 (71.54%), Query Frame = 0

Query: 6   VLLCMSFIFVMFSGCYYHKRKHSNG-KAAELPPGPKPWPLVGCLPTMLATNLPTYKWIHA 65
           + L ++FI  MF   +  K  + N      LPPGP PWP+VG LP M   N P ++WIH+
Sbjct: 16  ISLSLAFIIFMFLVKFILKTHNKNSVPVVPLPPGPSPWPIVGSLPEMW-RNRPAHRWIHS 75

Query: 66  VMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRCVTTI 125
           +MK+ NT+IACIRLG+ ++IPV S EIA EFLK  DA+FASR PV+ A   L+   +TT+
Sbjct: 76  LMKKLNTDIACIRLGNVHVIPVTSPEIAREFLKKNDAVFASR-PVTMATKTLSSGYLTTV 135

Query: 126 FAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF------RGGSVVNVRS 185
             P G+QWRKMRR+L  E  NPS +H +LG RN EAD L+++++      + G+VVNVR 
Sbjct: 136 VGPWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEADNLVKFLYNQCSANQNGAVVNVRI 195

Query: 186 ITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLK 245
             Q Y G+IMR+M+FN+ Y+GKGR+DGGP  EEEEH  ALLT++ + +AF +SD++PWL+
Sbjct: 196 AAQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEHVSALLTLLTYAYAFCVSDYLPWLR 255

Query: 246 PFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPL 305
            FD+DGHEK  +KA+N+V+K+ EPI++ER+Q W+D K  E  D++LD+ I L+  N +PL
Sbjct: 256 VFDIDGHEKKVRKAMNIVKKHQEPIVNERLQEWRDGKRNE-PDDLLDVFISLKDANGQPL 315

Query: 306 LSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHD 365
           LS  EIKAQ  ELQL+T+D+P N  EWA+ E++NQP++L+KA EELD+VVG++ LV+E  
Sbjct: 316 LSDEEIKAQTTELQLSTVDSPFNIAEWALTEMLNQPEMLKKAEEELDRVVGKKTLVQESH 375

Query: 366 IPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIW 425
           +P L Y+ AC +E  RLHP  PFN+PHVS  D  VAGYFIPKGS V+LSR  LGRNPR+W
Sbjct: 376 VPHLPYIRACAKEVMRLHPVGPFNLPHVSIADAEVAGYFIPKGSNVILSRLELGRNPRVW 435

Query: 426 EDPMRFNPERHMNDRT-RELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGF 485
           E+P+RFNPERH+N    +++ L E DLRF+SF  GRRGC G  LG+ I +ML ARLL GF
Sbjct: 436 EEPLRFNPERHLNIAVDQQVDLEENDLRFVSFSTGRRGCMGVGLGSTIVVMLLARLLQGF 495

Query: 486 SWTLLPGITKIDFFQAE-ELSLPKPLHLHAQPRLSHIMYP 517
           SW+L P + KIDF + +  L    PL   A+PRL   +YP
Sbjct: 496 SWSLPPDVDKIDFTEDQIYLKKASPLLAQAKPRLPASVYP 532

BLAST of CmaCh10G009000 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 3.4e-153
Identity = 268/518 (51.74%), Postives = 367/518 (70.85%), Query Frame = 0

Query: 7   LLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYKWIHAVM 66
           LL + F F++F     H    S  K  +LPPGPKPWP+VG LP MLA N P   WIH +M
Sbjct: 19  LLVVIFSFMIFKVTKTHLVNKS--KKYKLPPGPKPWPIVGNLPEMLA-NRPATIWIHKLM 78

Query: 67  KQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRCVTTIFA 126
           K+ NTEIACIRL +  +IPV    IA EFLK +DA FASRP +  + DI +   +TT+  
Sbjct: 79  KEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIM-STDIASDGFITTVLV 138

Query: 127 PMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFR--------GGSVVNVRS 186
           P GEQW+KM+R+L   +L+P     +LG RN EAD L+ YI+         G  +VN+R 
Sbjct: 139 PYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGPGLVNIRI 198

Query: 187 ITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLK 246
             QHY GN+ R+++FN RY+GK  +DGGP FEE EH  A  TI+++++AFSISDF+P+L+
Sbjct: 199 AAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSISDFVPFLR 258

Query: 247 PFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPL 306
             DLDGH     KA+ +++KY +PIID+R+++W D  L+ V++++LD+LI L+  N KPL
Sbjct: 259 RLDLDGHRSKIMKAMRIMRKYHDPIIDDRIKQWND-GLKTVEEDLLDVLIKLKDANNKPL 318

Query: 307 LSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHD 366
           L++ E+KAQI+EL +  +DNPSNA EWA+AE++NQP++L++A EELD VVG+ERLV+E D
Sbjct: 319 LTLKELKAQIIELAIEMVDNPSNAFEWALAEMINQPELLKRATEELDNVVGKERLVQESD 378

Query: 367 IPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIW 426
           IPKL+++ AC RE+ RLHP   FNVPH+   D +V  Y  PKG++VLLSR  LGRNP+ W
Sbjct: 379 IPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRVALGRNPKFW 438

Query: 427 EDPMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFS 486
            DP++FNPERH+ +   ++ L EPDLRF+SF  GRR CPG +LGT +T+MLFAR+LHGFS
Sbjct: 439 TDPLKFNPERHLKEGI-DVVLTEPDLRFISFTTGRRSCPGVALGTTMTVMLFARMLHGFS 498

Query: 487 WTLLPGITKIDFFQA-EELSLPKPLHLHAQPRLSHIMY 516
           W+  P ++ ID   + ++L L KPL L A+PRL+  +Y
Sbjct: 499 WSPPPDVSSIDLVPSKDDLFLAKPLLLVAKPRLAAELY 530

BLAST of CmaCh10G009000 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.9e-152
Identity = 266/518 (51.35%), Postives = 366/518 (70.66%), Query Frame = 0

Query: 7   LLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYKWIHAVM 66
           LL + F F++F     H    S  K  +LPPGPKPWP+VG LP MLA N P   WIH +M
Sbjct: 19  LLVVIFFFIIFKVTKSHSVNKS--KKYKLPPGPKPWPIVGNLPEMLA-NRPATIWIHKLM 78

Query: 67  KQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRCVTTIFA 126
           K+ NTEIACIRL +  +IPV    IA EFLK +DA FASRP +  + DI +   +TT+  
Sbjct: 79  KEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIM-STDIASDGFLTTVLV 138

Query: 127 PMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFR--------GGSVVNVRS 186
           P GEQW+KM+R+L   +L+P     +LG RN EAD L+ YI+         G  +VN+R 
Sbjct: 139 PYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGPGLVNIRI 198

Query: 187 ITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLK 246
             QHY GN+ R+++FN RY+GK  +DGGP FEE EH  A  TI+++++AFSISDF+P+L+
Sbjct: 199 AAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSISDFIPFLR 258

Query: 247 PFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPL 306
             DLDGH     KA+ +++KY +PII +R+++W D  L+ V++++LD+LI L+  + KPL
Sbjct: 259 RLDLDGHRSKIMKAMGIMKKYHDPIIHDRIKQWND-GLKTVEEDLLDVLIKLKDASNKPL 318

Query: 307 LSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHD 366
           L++ EIKAQI EL +  +DNPSNA EWA+AE++NQP++L++A EELD VVG+ERLV+E D
Sbjct: 319 LTLKEIKAQITELAIEMVDNPSNAFEWALAEMLNQPELLKRATEELDNVVGKERLVQESD 378

Query: 367 IPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIW 426
           IPKL+++ AC RE+ RLHP   FNVPH+   D +V  Y  PKG++VLLSR  LGRNP+ W
Sbjct: 379 IPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRVALGRNPKFW 438

Query: 427 EDPMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFS 486
            DP++FNPERH+ +   ++ L EPDLRF+SF  GRR CPG +LGT +T+MLFAR+LHGFS
Sbjct: 439 TDPLKFNPERHLKEGI-DVVLTEPDLRFISFTTGRRSCPGVTLGTTMTIMLFARMLHGFS 498

Query: 487 WTLLPGITKIDFFQ-AEELSLPKPLHLHAQPRLSHIMY 516
           W+  P ++ ID  Q +++L + KPL + A+PRL+  +Y
Sbjct: 499 WSAPPNVSSIDLTQSSDDLFMAKPLCVVAKPRLAAELY 530

BLAST of CmaCh10G009000 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 7.1e-143
Identity = 241/492 (48.98%), Postives = 349/492 (70.93%), Query Frame = 0

Query: 35  LPPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIACIRLGSNYIIPVASQEIALE 94
           LPPGPK WPL+G LP +L  N P ++WIH++MK+ NT+IACIRL + ++IPV S  IA E
Sbjct: 40  LPPGPKSWPLIGNLPEILGRNKPVFRWIHSLMKELNTDIACIRLANTHVIPVTSPRIARE 99

Query: 95  FLKTYDAIFASRPPVSNAVDILNGRCVTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLG 154
            LK  D++FA+R P++   +  +   +T    P GEQW+KMRR++A  V +  +   ML 
Sbjct: 100 ILKKQDSVFATR-PLTMGTEYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQ 159

Query: 155 HRNAEADTLLRYI------FRGGS--VVNVRSITQHYCGNIMRRMLFNQRYYGKGRDDG- 214
            R  EAD L+RYI       RG +  V+++R   + Y GN+ R+M+F  R++GKG +DG 
Sbjct: 160 KRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDGS 219

Query: 215 GPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPFDLDGHEKVTKKALNVVQKYDEPIID 274
           GP  EE EH ++L T++ H++AF++SD++PWL+  DL+GHEKV   A+  V KY++P +D
Sbjct: 220 GPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVD 279

Query: 275 ERVQRWKDEKLEEVKDNILDILILLQGENAKPLLSVAEIKAQIMELQLATIDNPSNAVEW 334
           ER+ +W++ K++E +D  LD+ I+ +  + KP LS  EIKAQ+ EL LAT+DNPSNA EW
Sbjct: 280 ERLMQWRNGKMKEPQD-FLDMFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPSNAAEW 339

Query: 335 AMAELVNQPKILEKAIEELDKVVGRERLVEEHDIPKLKYLTACIRESFRLHPFSPFNVPH 394
            MAE++N+P I++KA+EE+D+VVG++RLV E D+P L Y+ AC++E+FRLHP +PFN+PH
Sbjct: 340 GMAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPH 399

Query: 395 VSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNPERHMNDRTRELGLLEPDLR 454
           +S TD +V GYFIPKGS VL+SR G+GRNP +W+ P +F+PERH++  T  + L E DL 
Sbjct: 400 MSTTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTC-VDLNESDLN 459

Query: 455 FMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWTLLPGITKIDFFQAE-ELSLPKPLHL 514
            +SF  GRRGC G  +G+ +T ML ARL+ GF+W  +PG  KID  +++ +L + KPL+ 
Sbjct: 460 IISFSAGRRGCMGVDIGSAMTYMLLARLIQGFTWLPVPGKNKIDISESKNDLFMAKPLYA 519

Query: 515 HAQPRLSHIMYP 517
            A PRL+  +YP
Sbjct: 520 VATPRLAPHVYP 528

BLAST of CmaCh10G009000 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 501.5 bits (1290), Expect = 1.1e-140
Identity = 237/490 (48.37%), Postives = 343/490 (70.00%), Query Frame = 0

Query: 35  LPPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIACIRLGSNYIIPVASQEIALE 94
           LPPGP PWP+VG LP ML  N P ++WIH +M++  T+IAC++LG  +++ +   EIA E
Sbjct: 68  LPPGPVPWPVVGNLPEML-LNKPAFRWIHQMMREMGTDIACVKLGGVHVVSITCPEIARE 127

Query: 95  FLKTYDAIFASRPPVSNAVDILNGRCVTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLG 154
            L+  DA F SR P++ A +  +G     + +P G+QW+KMRR+L  E++ PS    +  
Sbjct: 128 VLRKQDANFISR-PLTFASETFSGGYRNAVLSPYGDQWKKMRRVLTSEIICPSRHAWLHD 187

Query: 155 HRNAEADTLLRYIFR-------GGSVVNVRSITQHYCGNIMRRMLFNQRYYGKGRDDGGP 214
            R  EAD L RY++        G   V+VR + +HYCGN++RR++FN+RY+G+ + DGGP
Sbjct: 188 KRTDEADNLTRYVYNLATKAATGDVAVDVRHVARHYCGNVIRRLMFNRRYFGEPQADGGP 247

Query: 215 TFEEEEHDQALLTIIRHIHAFSISDFMPWLKPFDLDGHEKVTKKALNVVQKYDEPIIDER 274
              E  H  A+ T +  ++AF +SD++PWL+  DLDGHEK+ K+A   V +  + +ID+R
Sbjct: 248 GPMEVLHMDAVFTSLGLLYAFCVSDYLPWLRGLDLDGHEKIVKEANVAVNRLHDTVIDDR 307

Query: 275 VQRWKDEKLEEVKDNILDILILLQGENAKPLLSVAEIKAQIMELQLATIDNPSNAVEWAM 334
            ++WK  + +E++D  LD+LI L+     PLL++ E+KAQ  ++  A +DNPSNAVEWA+
Sbjct: 308 WRQWKSGERQEMED-FLDVLITLKDAQGNPLLTIEEVKAQSQDITFAAVDNPSNAVEWAL 367

Query: 335 AELVNQPKILEKAIEELDKVVGRERLVEEHDIPKLKYLTACIRESFRLHPFSPFNVPHVS 394
           AE+VN P+++ KA+EELD+VVGRERLV+E DIPKL Y+ ACIRE+FRLHP +PFNVPHV+
Sbjct: 368 AEMVNNPEVMAKAMEELDRVVGRERLVQESDIPKLNYVKACIREAFRLHPVAPFNVPHVA 427

Query: 395 NTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNPERHMNDRTRELGLLEPDLRFM 454
             D  +AGY +PKGS V+LSRTGLGRNPR+W++P+RF P+RH+     ++ L E DLRF+
Sbjct: 428 LADTTIAGYRVPKGSHVILSRTGLGRNPRVWDEPLRFYPDRHLATAASDVALTENDLRFI 487

Query: 455 SFGRGRRGCPGSSLGTNITMMLFARLLHGFSWTLLPGITKIDFFQAE-ELSLPKPLHLHA 514
           SF  GRRGC  +SLGT +++MLF RLL GF+W+   G+  +D  +++ +  +  PL LHA
Sbjct: 488 SFSTGRRGCIAASLGTAMSVMLFGRLLQGFTWSKPAGVEAVDLSESKSDTFMATPLVLHA 547

Query: 515 QPRLSHIMYP 517
           +PRL   +YP
Sbjct: 548 EPRLPAHLYP 554

BLAST of CmaCh10G009000 vs. ExPASy TrEMBL
Match: A0A6J1JIL4 (isoleucine N-monooxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485430 PE=3 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.5e-308
Identity = 521/521 (100.00%), Postives = 521/521 (100.00%), Query Frame = 0

Query: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60
           MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK
Sbjct: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60

Query: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120
           WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC
Sbjct: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120

Query: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFRGGSVVNVRSIT 180
           VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFRGGSVVNVRSIT
Sbjct: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFRGGSVVNVRSIT 180

Query: 181 QHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPF 240
           QHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPF
Sbjct: 181 QHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPF 240

Query: 241 DLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPLLS 300
           DLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPLLS
Sbjct: 241 DLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPLLS 300

Query: 301 VAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIP 360
           VAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIP
Sbjct: 301 VAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIP 360

Query: 361 KLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWED 420
           KLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWED
Sbjct: 361 KLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWED 420

Query: 421 PMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWT 480
           PMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWT
Sbjct: 421 PMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWT 480

Query: 481 LLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPKACSS 522
           LLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPKACSS
Sbjct: 481 LLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPKACSS 521

BLAST of CmaCh10G009000 vs. ExPASy TrEMBL
Match: A0A6J1H7W7 (isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461341 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 6.9e-274
Identity = 466/521 (89.44%), Postives = 488/521 (93.67%), Query Frame = 0

Query: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60
           MDAFLVLLCM FIFVMFS CYY K KHSNG+AAELPPGPKPWPLVGC PTMLATNLPTYK
Sbjct: 1   MDAFLVLLCMPFIFVMFSHCYYRKTKHSNGQAAELPPGPKPWPLVGCRPTMLATNLPTYK 60

Query: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120
           WIHAVMKQFNTEIACIRLGS YIIPVAS  +ALEFLKTYD+IFASR  +SN VDILN RC
Sbjct: 61  WIHAVMKQFNTEIACIRLGSTYIIPVASPRLALEFLKTYDSIFASRSSISNTVDILNRRC 120

Query: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF-----RGGSVVN 180
           VTTIFAP+G+QWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF     RGG VVN
Sbjct: 121 VTTIFAPLGQQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFSQTSTRGGGVVN 180

Query: 181 VRSITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMP 240
           VRS+TQHYCGNIMRRMLFNQRYYGK R+DGGPTFEEEEHDQA LTIIRH+HAFSISDFM 
Sbjct: 181 VRSVTQHYCGNIMRRMLFNQRYYGKWREDGGPTFEEEEHDQASLTIIRHLHAFSISDFMS 240

Query: 241 WLKPFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENA 300
           WLKPFDLDGHEKV K ALNVVQK+DEPIIDERVQRWKDEKLEEVKD+ILDILI L+ ENA
Sbjct: 241 WLKPFDLDGHEKVMKSALNVVQKFDEPIIDERVQRWKDEKLEEVKDDILDILISLKDENA 300

Query: 301 KPLLSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVE 360
           KPLLSV EIKAQIMELQ+ATIDNPSNAVEWAMAEL+NQPKILEKAIEELDKVVGRERLV+
Sbjct: 301 KPLLSVVEIKAQIMELQIATIDNPSNAVEWAMAELLNQPKILEKAIEELDKVVGRERLVQ 360

Query: 361 EHDIPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP 420
           E+DIP LKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP
Sbjct: 361 EYDIPNLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP 420

Query: 421 RIWEDPMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLH 480
           RIWEDPMRFN ERH+NDRT ELGLLEP+LRFMSF RGRRGCPGSSLGTNITMMLFARLL 
Sbjct: 421 RIWEDPMRFNRERHLNDRTVELGLLEPNLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQ 480

Query: 481 GFSWTLLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYP 517
           GFSW+LLPGITKIDF QA+ELSLPKPLHL A+PRLSH+MYP
Sbjct: 481 GFSWSLLPGITKIDFSQADELSLPKPLHLLAKPRLSHVMYP 521

BLAST of CmaCh10G009000 vs. ExPASy TrEMBL
Match: A0A6J1H9V7 (isoleucine N-monooxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461429 PE=3 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 6.7e-261
Identity = 440/522 (84.29%), Postives = 480/522 (91.95%), Query Frame = 0

Query: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60
           MDAF VLL M FIFVMFS CYY KRKHSNG+A ELPPGPKPWPLVGCLPTML TNLPTY+
Sbjct: 1   MDAFFVLLFMPFIFVMFSQCYYRKRKHSNGEAPELPPGPKPWPLVGCLPTMLTTNLPTYQ 60

Query: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120
           WIHAVMKQFN EIACIRLGS YIIPVAS ++ALEFLKTYD+IFASR  +SN VD L+ R 
Sbjct: 61  WIHAVMKQFNAEIACIRLGSIYIIPVASPQLALEFLKTYDSIFASRSSISNTVDTLSHRY 120

Query: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF-----RGGSVVN 180
           +T  F+P+G+QWRKMRRILA E+LN STLHRMLGHRNAEADTLLRYIF     R G+VVN
Sbjct: 121 LTAAFSPLGKQWRKMRRILASEILNSSTLHRMLGHRNAEADTLLRYIFSQTSNRDGAVVN 180

Query: 181 VRSITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMP 240
           +RSITQHYCGNI+RRMLFN+RYYGKGR+DGGPTFEEEEH+QALLTI++H++AFS SDFMP
Sbjct: 181 LRSITQHYCGNIIRRMLFNKRYYGKGREDGGPTFEEEEHNQALLTILKHVNAFSTSDFMP 240

Query: 241 WLKPFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENA 300
           WLKPFDLDGH+K+ K ALNVVQKYDEPIIDERVQRWKD K+E VK++IL+ILI L+ ENA
Sbjct: 241 WLKPFDLDGHQKIMKNALNVVQKYDEPIIDERVQRWKDGKMEGVKEDILNILISLKDENA 300

Query: 301 KPLLSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVE 360
           KPLLSVAEIKAQI ELQ+ATIDNPSNAVEWAMAEL+NQPKILEKAIEELDKVVGRERLV+
Sbjct: 301 KPLLSVAEIKAQITELQIATIDNPSNAVEWAMAELLNQPKILEKAIEELDKVVGRERLVQ 360

Query: 361 EHDIPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP 420
           E+DIP LKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSR  LGRNP
Sbjct: 361 EYDIPNLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRISLGRNP 420

Query: 421 RIWEDPMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLH 480
           RIWEDPMRFNPERH+NDRT ELGLLEP+LRFMSF RGRRGCPGSSLGTNITMMLFARLL 
Sbjct: 421 RIWEDPMRFNPERHLNDRTVELGLLEPNLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQ 480

Query: 481 GFSWTLLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPK 518
           GFSW+ +PGITKIDF +A+ELSLPKPLHLHAQPRLSHIMYP+
Sbjct: 481 GFSWSFVPGITKIDFSEADELSLPKPLHLHAQPRLSHIMYPR 522

BLAST of CmaCh10G009000 vs. ExPASy TrEMBL
Match: A0A6J1H8D3 (LOW QUALITY PROTEIN: isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461430 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 4.9e-256
Identity = 430/507 (84.81%), Postives = 471/507 (92.90%), Query Frame = 0

Query: 16  MFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIAC 75
           MFS CYY K KHSNG+AAE+PPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIAC
Sbjct: 1   MFSHCYYRKTKHSNGEAAEIPPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIAC 60

Query: 76  IRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRCVTTIFAPMGEQWRKM 135
           IRLGS Y + VAS ++ALEFLKTYD+IFASR  +SN VDIL+ R +T  F+P+GEQW+KM
Sbjct: 61  IRLGSTY-LXVASPQLALEFLKTYDSIFASRSSISNTVDILSHRFLTAAFSPLGEQWKKM 120

Query: 136 RRILALEVLNPSTLHRMLGHRNAEADTLLRYIF-----RGGSVVNVRSITQHYCGNIMRR 195
           RRILALE+LNPSTLHRMLGHR+ EAD LLRYIF     RGG+VVNVRSITQHYCGNI+RR
Sbjct: 121 RRILALEILNPSTLHRMLGHRDTEADNLLRYIFSQTSNRGGAVVNVRSITQHYCGNIIRR 180

Query: 196 MLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPFDLDGHEKVTK 255
           MLFNQRYYGKGR+DGGPTFEEEEH+QALLTI+RH++AFSISDF+PWLKPFDLDGH+KV K
Sbjct: 181 MLFNQRYYGKGREDGGPTFEEEEHNQALLTILRHVNAFSISDFLPWLKPFDLDGHQKVMK 240

Query: 256 KALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPLLSVAEIKAQIME 315
            ALNVVQKYDEPIID+RVQ+W+DEKLEEVKD+ILDILI L+ ENAKPLLSVAEIKAQI E
Sbjct: 241 NALNVVQKYDEPIIDKRVQKWEDEKLEEVKDDILDILISLKDENAKPLLSVAEIKAQITE 300

Query: 316 LQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIPKLKYLTACIR 375
           LQ+ATIDNPSNAVEWAMAEL+NQPKIL+KAIEELDKVVGRERLVEE+DIP LKYLTACIR
Sbjct: 301 LQIATIDNPSNAVEWAMAELLNQPKILKKAIEELDKVVGRERLVEEYDIPNLKYLTACIR 360

Query: 376 ESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNPERHM 435
           ESFRLHPFSPFN+PHVSN+DIIVAGYFIPKGSEVLLSR GLGRNPR+WEDPM+FNPERHM
Sbjct: 361 ESFRLHPFSPFNIPHVSNSDIIVAGYFIPKGSEVLLSRIGLGRNPRVWEDPMKFNPERHM 420

Query: 436 NDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWTLLPGITKIDF 495
           NDRT ELGLLEPDLRFMSF RGRRGCPGSSLGTNITMMLFARLL GFSW+LL  ITKIDF
Sbjct: 421 NDRTTELGLLEPDLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQGFSWSLLREITKIDF 480

Query: 496 FQAEELSLPKPLHLHAQPRLSHIMYPK 518
            + +ELSLPKPLHLHA+PRLSHIMY +
Sbjct: 481 SEVDELSLPKPLHLHAKPRLSHIMYTR 506

BLAST of CmaCh10G009000 vs. ExPASy TrEMBL
Match: A0A6J1H9G3 (isoleucine N-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461343 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.7e-251
Identity = 419/522 (80.27%), Postives = 467/522 (89.46%), Query Frame = 0

Query: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60
           MDAF VLLCMSFIFVMFS CYY K KHSNG+AAELPPGPKPWPLVGCLPTML TN+PTYK
Sbjct: 1   MDAFFVLLCMSFIFVMFSQCYYRKTKHSNGEAAELPPGPKPWPLVGCLPTMLTTNMPTYK 60

Query: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120
           WIHAVMKQFNTEIACIRLGS YIIPV+S EIALEFLK  D+IFASR  +SN V +L+   
Sbjct: 61  WIHAVMKQFNTEIACIRLGSTYIIPVSSPEIALEFLKINDSIFASRSCISNTVGMLSHGY 120

Query: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF-----RGGSVVN 180
           +TT  +PMGEQWRKMRRIL  E+ NPSTLHRM+GHRN EADTLLRY+F     R G VVN
Sbjct: 121 LTTALSPMGEQWRKMRRILTSEIFNPSTLHRMVGHRNIEADTLLRYVFSQTSNRDGGVVN 180

Query: 181 VRSITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMP 240
           +RSITQHYCGNIMRRM+FN+RYYGKGR+DGGPTFEEEEH+QAL T+I +I+AFSISDF+P
Sbjct: 181 IRSITQHYCGNIMRRMIFNKRYYGKGREDGGPTFEEEEHNQALFTMIEYIYAFSISDFVP 240

Query: 241 WLKPFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENA 300
           WLKPFDLDGH+K+ KKALNVVQKYDEPIIDERVQRWKD K+E VKD+ILDILI L+ ENA
Sbjct: 241 WLKPFDLDGHQKIMKKALNVVQKYDEPIIDERVQRWKDGKMEGVKDDILDILISLKDENA 300

Query: 301 KPLLSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVE 360
           KP+LSVAEIKAQI ELQ+A IDNPSN VEWAMAEL+N+PKILEKA+EELDKV+GRER+VE
Sbjct: 301 KPMLSVAEIKAQITELQIAAIDNPSNIVEWAMAELMNEPKILEKAMEELDKVIGRERVVE 360

Query: 361 EHDIPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP 420
           E DIP LKYLTACIRE+FR+H FSPFN+PHVSN+DI +AGYFIPKGSEV LSR GLGRNP
Sbjct: 361 ESDIPNLKYLTACIRETFRIHQFSPFNLPHVSNSDITIAGYFIPKGSEVFLSRIGLGRNP 420

Query: 421 RIWEDPMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLH 480
           R WEDPM+FNPERHM D T ELGLLEP+LRF++F RGRRGC GSSLGT ITMMLFARLL 
Sbjct: 421 RTWEDPMKFNPERHMKDTTTELGLLEPNLRFITFSRGRRGCLGSSLGTTITMMLFARLLQ 480

Query: 481 GFSWTLLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPK 518
           GFSW+LLPGITKIDF +A+ELSLPKPLHLH +PRLSH+MYP+
Sbjct: 481 GFSWSLLPGITKIDFSEADELSLPKPLHLHGKPRLSHVMYPR 522

BLAST of CmaCh10G009000 vs. NCBI nr
Match: XP_022988080.1 (isoleucine N-monooxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 1067.8 bits (2760), Expect = 3.0e-308
Identity = 521/521 (100.00%), Postives = 521/521 (100.00%), Query Frame = 0

Query: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60
           MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK
Sbjct: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60

Query: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120
           WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC
Sbjct: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120

Query: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFRGGSVVNVRSIT 180
           VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFRGGSVVNVRSIT
Sbjct: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFRGGSVVNVRSIT 180

Query: 181 QHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPF 240
           QHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPF
Sbjct: 181 QHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPF 240

Query: 241 DLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPLLS 300
           DLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPLLS
Sbjct: 241 DLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPLLS 300

Query: 301 VAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIP 360
           VAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIP
Sbjct: 301 VAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIP 360

Query: 361 KLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWED 420
           KLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWED
Sbjct: 361 KLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWED 420

Query: 421 PMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWT 480
           PMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWT
Sbjct: 421 PMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWT 480

Query: 481 LLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPKACSS 522
           LLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPKACSS
Sbjct: 481 LLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPKACSS 521

BLAST of CmaCh10G009000 vs. NCBI nr
Match: XP_023516712.1 (isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 966.8 bits (2498), Expect = 7.3e-278
Identity = 468/522 (89.66%), Postives = 492/522 (94.25%), Query Frame = 0

Query: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60
           MDAFLVLLCM FIFVMFS CYYHKRKHSNG+AAELPPGPKPWPLVGCLPTMLATN+PTYK
Sbjct: 1   MDAFLVLLCMPFIFVMFSHCYYHKRKHSNGEAAELPPGPKPWPLVGCLPTMLATNMPTYK 60

Query: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120
           WIHAVMKQFNTEIACIRLGS YIIPV S ++ALEFLKTYD+IFASR  VSN VDILN RC
Sbjct: 61  WIHAVMKQFNTEIACIRLGSTYIIPVTSPQLALEFLKTYDSIFASRSSVSNTVDILNRRC 120

Query: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF-----RGGSVVN 180
           VTTIFAP+G+QWRKMRRILALEVLNPSTLHRMLGHRNAE DTLLRYIF     RGG VVN
Sbjct: 121 VTTIFAPLGQQWRKMRRILALEVLNPSTLHRMLGHRNAEVDTLLRYIFSQTSTRGGGVVN 180

Query: 181 VRSITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMP 240
           VRSITQHYCGNIMRRMLFNQRYYGKGR+DGGPTFEEEEHDQAL TIIRH+HAFSISDFMP
Sbjct: 181 VRSITQHYCGNIMRRMLFNQRYYGKGREDGGPTFEEEEHDQALFTIIRHVHAFSISDFMP 240

Query: 241 WLKPFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENA 300
           WLKPFDLDGHEK+ KKALN+VQKYDEPIIDERVQ+WKDEKLE VKD+ILDILI L+ ENA
Sbjct: 241 WLKPFDLDGHEKIMKKALNIVQKYDEPIIDERVQKWKDEKLEGVKDDILDILISLKDENA 300

Query: 301 KPLLSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVE 360
           KPLLSVAEIKAQI ELQ+ATIDNPSNAVEWAMAEL+NQPK LEKAIEELDKVVGRERLVE
Sbjct: 301 KPLLSVAEIKAQITELQIATIDNPSNAVEWAMAELLNQPKFLEKAIEELDKVVGRERLVE 360

Query: 361 EHDIPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP 420
           E+DIP LKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLG+NP
Sbjct: 361 EYDIPNLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGQNP 420

Query: 421 RIWEDPMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLH 480
           RIWEDPMRFNPERH+NDRT +LGLLEP+LRFMSF RGRRGCPGSSLGTNITMMLFARLL 
Sbjct: 421 RIWEDPMRFNPERHLNDRTMKLGLLEPNLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQ 480

Query: 481 GFSWTLLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPK 518
           GFSW+L+PGITKID  QA ELSLPKPLHLHAQPRLSHIMYP+
Sbjct: 481 GFSWSLVPGITKIDLSQAHELSLPKPLHLHAQPRLSHIMYPR 522

BLAST of CmaCh10G009000 vs. NCBI nr
Match: KAG6590174.1 (Isoleucine N-monooxygenase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 958.7 bits (2477), Expect = 2.0e-275
Identity = 467/522 (89.46%), Postives = 490/522 (93.87%), Query Frame = 0

Query: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60
           MDAFLVLLCM FIFVMFS CYY K KHSNG+AAELPPGPKPWPLVGCLPTMLATNLPTYK
Sbjct: 1   MDAFLVLLCMPFIFVMFSHCYYRKTKHSNGQAAELPPGPKPWPLVGCLPTMLATNLPTYK 60

Query: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120
           WIHAVMKQFNTEIACIRLGS YIIPVAS  +ALEFLKTYD+IFASR  +SN VDILN RC
Sbjct: 61  WIHAVMKQFNTEIACIRLGSTYIIPVASPRLALEFLKTYDSIFASRSSISNTVDILNRRC 120

Query: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF-----RGGSVVN 180
           VTTIFAP+G+QWRKMRRILALEVLNPSTLHRML HRNAEADTLLRYIF     RGG VVN
Sbjct: 121 VTTIFAPLGQQWRKMRRILALEVLNPSTLHRMLDHRNAEADTLLRYIFSQTSTRGGGVVN 180

Query: 181 VRSITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMP 240
           VRS+TQHYCGNIMRRMLFNQRYYGK R+DGGPTFEEEEHDQALLTIIRH+HAFSISDFM 
Sbjct: 181 VRSVTQHYCGNIMRRMLFNQRYYGKWREDGGPTFEEEEHDQALLTIIRHLHAFSISDFMS 240

Query: 241 WLKPFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENA 300
           WLKPFDLD HEKV K ALNV+QKYDEPIIDERVQRWKDEKLEEVKD+ILDILI L+ ENA
Sbjct: 241 WLKPFDLDEHEKVMKNALNVIQKYDEPIIDERVQRWKDEKLEEVKDDILDILISLKDENA 300

Query: 301 KPLLSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVE 360
           KPLLSV EIKAQIMELQ+ATIDNPSNAVEWAMAEL+NQPKILEKAIEELDKVVGRERLVE
Sbjct: 301 KPLLSVVEIKAQIMELQIATIDNPSNAVEWAMAELLNQPKILEKAIEELDKVVGRERLVE 360

Query: 361 EHDIPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP 420
           E+DIP LKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAG+FIPKGSEVLLSRTGLGRNP
Sbjct: 361 EYDIPNLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGFFIPKGSEVLLSRTGLGRNP 420

Query: 421 RIWEDPMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLH 480
           RIWEDPMRFNPERH+NDRT ELGLLEP+LRFMSF RGRRGCPGSSLGTNITMMLFARLL 
Sbjct: 421 RIWEDPMRFNPERHLNDRTVELGLLEPNLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQ 480

Query: 481 GFSWTLLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPK 518
           GFSW+ +PGITKIDF +A+ELSLPKPLHLHAQPRLSHIMYP+
Sbjct: 481 GFSWSFVPGITKIDFSEADELSLPKPLHLHAQPRLSHIMYPR 522

BLAST of CmaCh10G009000 vs. NCBI nr
Match: XP_022960607.1 (isoleucine N-monooxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 952.6 bits (2461), Expect = 1.4e-273
Identity = 466/521 (89.44%), Postives = 488/521 (93.67%), Query Frame = 0

Query: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60
           MDAFLVLLCM FIFVMFS CYY K KHSNG+AAELPPGPKPWPLVGC PTMLATNLPTYK
Sbjct: 1   MDAFLVLLCMPFIFVMFSHCYYRKTKHSNGQAAELPPGPKPWPLVGCRPTMLATNLPTYK 60

Query: 61  WIHAVMKQFNTEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRC 120
           WIHAVMKQFNTEIACIRLGS YIIPVAS  +ALEFLKTYD+IFASR  +SN VDILN RC
Sbjct: 61  WIHAVMKQFNTEIACIRLGSTYIIPVASPRLALEFLKTYDSIFASRSSISNTVDILNRRC 120

Query: 121 VTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF-----RGGSVVN 180
           VTTIFAP+G+QWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF     RGG VVN
Sbjct: 121 VTTIFAPLGQQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIFSQTSTRGGGVVN 180

Query: 181 VRSITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMP 240
           VRS+TQHYCGNIMRRMLFNQRYYGK R+DGGPTFEEEEHDQA LTIIRH+HAFSISDFM 
Sbjct: 181 VRSVTQHYCGNIMRRMLFNQRYYGKWREDGGPTFEEEEHDQASLTIIRHLHAFSISDFMS 240

Query: 241 WLKPFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENA 300
           WLKPFDLDGHEKV K ALNVVQK+DEPIIDERVQRWKDEKLEEVKD+ILDILI L+ ENA
Sbjct: 241 WLKPFDLDGHEKVMKSALNVVQKFDEPIIDERVQRWKDEKLEEVKDDILDILISLKDENA 300

Query: 301 KPLLSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVE 360
           KPLLSV EIKAQIMELQ+ATIDNPSNAVEWAMAEL+NQPKILEKAIEELDKVVGRERLV+
Sbjct: 301 KPLLSVVEIKAQIMELQIATIDNPSNAVEWAMAELLNQPKILEKAIEELDKVVGRERLVQ 360

Query: 361 EHDIPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP 420
           E+DIP LKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP
Sbjct: 361 EYDIPNLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNP 420

Query: 421 RIWEDPMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLH 480
           RIWEDPMRFN ERH+NDRT ELGLLEP+LRFMSF RGRRGCPGSSLGTNITMMLFARLL 
Sbjct: 421 RIWEDPMRFNRERHLNDRTVELGLLEPNLRFMSFSRGRRGCPGSSLGTNITMMLFARLLQ 480

Query: 481 GFSWTLLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYP 517
           GFSW+LLPGITKIDF QA+ELSLPKPLHL A+PRLSH+MYP
Sbjct: 481 GFSWSLLPGITKIDFSQADELSLPKPLHLLAKPRLSHVMYP 521

BLAST of CmaCh10G009000 vs. NCBI nr
Match: XP_023516709.1 (isoleucine N-monooxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 928.3 bits (2398), Expect = 2.9e-266
Identity = 453/536 (84.51%), Postives = 489/536 (91.23%), Query Frame = 0

Query: 1   MDAFLVLLCMSFIFVMFSGCYYHKRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYK 60
           MD FLVLLCM FIFVMFS CYY K KHSNG+AA+LPPGPKPWPLVGCLPTMLATN+PTYK
Sbjct: 1   MDVFLVLLCMPFIFVMFSHCYYRKTKHSNGEAAKLPPGPKPWPLVGCLPTMLATNMPTYK 60

Query: 61  WIHAVMKQFN--TEIACIRLGSNYIIPVASQEIALEFLKTYDAIFASRPPVSNAVDILNG 120
           WIHAVMKQFN  TEIACIRLGS YIIPVAS ++ALEFLKTYD+IFASR  VSN VDIL+ 
Sbjct: 61  WIHAVMKQFNTETEIACIRLGSTYIIPVASPQLALEFLKTYDSIFASRSSVSNTVDILSH 120

Query: 121 RCVTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLGHRNAEADTLLRYIF---------- 180
           R +T  F+P+GEQW+KMRRILALE+LNPSTLHRMLGHR+ EAD LLRYIF          
Sbjct: 121 RFLTAAFSPLGEQWKKMRRILALEILNPSTLHRMLGHRDTEADNLLRYIFSQTTDNLLRY 180

Query: 181 -------RGGSVVNVRSITQHYCGNIMRRMLFNQRYYGKGRDDGGPTFEEEEHDQALLTI 240
                  RGG+VVNVRSITQHYCGNI+RRMLFNQRYYGKGR+DGGPTFEEEEH+QALLTI
Sbjct: 181 IFSQTSNRGGAVVNVRSITQHYCGNIIRRMLFNQRYYGKGREDGGPTFEEEEHNQALLTI 240

Query: 241 IRHIHAFSISDFMPWLKPFDLDGHEKVTKKALNVVQKYDEPIIDERVQRWKDEKLEEVKD 300
           +RH++AFSISDF+PWLKPFDLDGH+KV K ALNVVQKYDEPIIDERVQRWKDEKLEEVKD
Sbjct: 241 LRHVNAFSISDFLPWLKPFDLDGHQKVLKNALNVVQKYDEPIIDERVQRWKDEKLEEVKD 300

Query: 301 NILDILILLQGENAKPLLSVAEIKAQIMELQLATIDNPSNAVEWAMAELVNQPKILEKAI 360
           +ILDILI L+ ENAKPLLSVAEIKAQI ELQ+ATIDNPSNAVEWAMAEL+NQPKILEKAI
Sbjct: 301 DILDILISLKDENAKPLLSVAEIKAQITELQIATIDNPSNAVEWAMAELLNQPKILEKAI 360

Query: 361 EELDKVVGRERLVEEHDIPKLKYLTACIRESFRLHPFSPFNVPHVSNTDIIVAGYFIPKG 420
           EELDKVVGRERLVEE+DIP LKYLTACIRESFRLHPFSPFN+PHVSN+DIIVAGYFIPKG
Sbjct: 361 EELDKVVGRERLVEEYDIPNLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVAGYFIPKG 420

Query: 421 SEVLLSRTGLGRNPRIWEDPMRFNPERHMNDRTRELGLLEPDLRFMSFGRGRRGCPGSSL 480
           SEVLLSR GLGRNP+IWEDPM+FNPERHMNDRT ELGLLEPDLRFMSF RGRRGCPGSSL
Sbjct: 421 SEVLLSRIGLGRNPKIWEDPMKFNPERHMNDRTTELGLLEPDLRFMSFSRGRRGCPGSSL 480

Query: 481 GTNITMMLFARLLHGFSWTLLPGITKIDFFQAEELSLPKPLHLHAQPRLSHIMYPK 518
           GTNITMMLFARLL GFSW+LL GITKIDF +A+ELSLPKPLHLHA+PRLSHIMYP+
Sbjct: 481 GTNITMMLFARLLQGFSWSLLRGITKIDFSEADELSLPKPLHLHAKPRLSHIMYPR 536

BLAST of CmaCh10G009000 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 508.8 bits (1309), Expect = 5.0e-144
Identity = 241/492 (48.98%), Postives = 349/492 (70.93%), Query Frame = 0

Query: 35  LPPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIACIRLGSNYIIPVASQEIALE 94
           LPPGPK WPL+G LP +L  N P ++WIH++MK+ NT+IACIRL + ++IPV S  IA E
Sbjct: 34  LPPGPKSWPLIGNLPEILGRNKPVFRWIHSLMKELNTDIACIRLANTHVIPVTSPRIARE 93

Query: 95  FLKTYDAIFASRPPVSNAVDILNGRCVTTIFAPMGEQWRKMRRILALEVLNPSTLHRMLG 154
            LK  D++FA+R P++   +  +   +T    P GEQW+KMRR++A  V +  +   ML 
Sbjct: 94  ILKKQDSVFATR-PLTMGTEYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQ 153

Query: 155 HRNAEADTLLRYI------FRGGS--VVNVRSITQHYCGNIMRRMLFNQRYYGKGRDDG- 214
            R  EAD L+RYI       RG +  V+++R   + Y GN+ R+M+F  R++GKG +DG 
Sbjct: 154 KRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDGS 213

Query: 215 GPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPFDLDGHEKVTKKALNVVQKYDEPIID 274
           GP  EE EH ++L T++ H++AF++SD++PWL+  DL+GHEKV   A+  V KY++P +D
Sbjct: 214 GPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVD 273

Query: 275 ERVQRWKDEKLEEVKDNILDILILLQGENAKPLLSVAEIKAQIMELQLATIDNPSNAVEW 334
           ER+ +W++ K++E +D  LD+ I+ +  + KP LS  EIKAQ+ EL LAT+DNPSNA EW
Sbjct: 274 ERLMQWRNGKMKEPQD-FLDMFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPSNAAEW 333

Query: 335 AMAELVNQPKILEKAIEELDKVVGRERLVEEHDIPKLKYLTACIRESFRLHPFSPFNVPH 394
            MAE++N+P I++KA+EE+D+VVG++RLV E D+P L Y+ AC++E+FRLHP +PFN+PH
Sbjct: 334 GMAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPH 393

Query: 395 VSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNPERHMNDRTRELGLLEPDLR 454
           +S TD +V GYFIPKGS VL+SR G+GRNP +W+ P +F+PERH++  T  + L E DL 
Sbjct: 394 MSTTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTC-VDLNESDLN 453

Query: 455 FMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWTLLPGITKIDFFQAE-ELSLPKPLHL 514
            +SF  GRRGC G  +G+ +T ML ARL+ GF+W  +PG  KID  +++ +L + KPL+ 
Sbjct: 454 IISFSAGRRGCMGVDIGSAMTYMLLARLIQGFTWLPVPGKNKIDISESKNDLFMAKPLYA 513

Query: 515 HAQPRLSHIMYP 517
            A PRL+  +YP
Sbjct: 514 VATPRLAPHVYP 522

BLAST of CmaCh10G009000 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 474.9 bits (1221), Expect = 8.1e-134
Identity = 236/498 (47.39%), Postives = 339/498 (68.07%), Query Frame = 0

Query: 24  KRKHSNGKAAELPPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIACIRLGSNYI 83
           K    N K   LPPGP  +P+VG +P ML  N P ++W+H++MK+ NTEIAC+RLG+ ++
Sbjct: 47  KSSSRNKKLHPLPPGPTGFPIVGMIPAML-KNRPVFRWLHSLMKELNTEIACVRLGNTHV 106

Query: 84  IPVASQEIALEFLKTYDAIFASRPPVSNAVDILNGRCVTTIFAPMGEQWRKMRRILALEV 143
           IPV   +IA E  K  DA+FASR P++ A  IL+    T +  P GEQ++KMR+++  E+
Sbjct: 107 IPVTCPKIAREIFKQQDALFASR-PLTYAQKILSNGYKTCVITPFGEQFKKMRKVIMTEI 166

Query: 144 LNPSTLHRMLGHRNAEADTLLRYIF---RGGSVVNVRSITQHYCGNIMRRMLFNQRYYG- 203
           + P+    +  +R  E D L  +++   +    V++R +T+HYCGN ++R++F  R +  
Sbjct: 167 VCPARHRWLHDNRAEETDHLTAWLYNMVKNSEPVDLRFVTRHYCGNAIKRLMFGTRTFSE 226

Query: 204 KGRDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPFDLDGHEKVTKKALNVVQKY 263
           K   DGGPT E+ EH  A+   +    AF ISD++P L   DL+GHEK+ +++  ++ KY
Sbjct: 227 KTEADGGPTLEDIEHMDAMFEGLGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKY 286

Query: 264 DEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPLLSVAEIKAQIMELQLATIDNP 323
            +PIIDER++ W++ K  +++D  LDI I ++ E  +PLL+  EIK  I EL +A  DNP
Sbjct: 287 HDPIIDERIKMWREGKRTQIED-FLDIFISIKDEAGQPLLTADEIKPTIKELVMAAPDNP 346

Query: 324 SNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIPKLKYLTACIRESFRLHPFS 383
           SNAVEWA+AE++N+P+IL KA+EE+D+VVG+ER V+E DIPKL Y+ A IRE+FRLHP +
Sbjct: 347 SNAVEWAIAEMINKPEILHKAMEEIDRVVGKERFVQESDIPKLNYVKAIIREAFRLHPVA 406

Query: 384 PFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNPERHMNDRTRELGL 443
            FN+PHV+ +D  VAGY IPKGS+VLLSR GLGRNP++W DP+ F PERH+N+   E+ L
Sbjct: 407 AFNLPHVALSDTTVAGYHIPKGSQVLLSRYGLGRNPKVWSDPLSFKPERHLNE-CSEVTL 466

Query: 444 LEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWTLLPGITKIDFFQ-AEELSL 503
            E DLRF+SF  G+RGC   +LGT IT M+ ARLL GF W L    T+++  + + ++ L
Sbjct: 467 TENDLRFISFSTGKRGCAAPALGTAITTMMLARLLQGFKWKLAGSETRVELMESSHDMFL 526

Query: 504 PKPLHLHAQPRLSHIMYP 517
            KPL L  + RLS  +YP
Sbjct: 527 SKPLVLVGELRLSEDLYP 540

BLAST of CmaCh10G009000 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 472.2 bits (1214), Expect = 5.2e-133
Identity = 232/493 (47.06%), Postives = 338/493 (68.56%), Query Frame = 0

Query: 29  NGKAAELPPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIACIRLGSNYIIPVAS 88
           N K   LPPGP  WP++G +PTML +  P ++W+H++MKQ NTEIAC++LG+ ++I V  
Sbjct: 50  NKKKPYLPPGPTGWPIIGMIPTMLKSR-PVFRWLHSIMKQLNTEIACVKLGNTHVITVTC 109

Query: 89  QEIALEFLKTYDAIFASRPPVSNAVDILNGRCVTTIFAPMGEQWRKMRRILALEVLNPST 148
            +IA E LK  DA+FASR P++ A  IL+    T +  P G+Q++KMR+++  E++ P+ 
Sbjct: 110 PKIAREILKQQDALFASR-PLTYAQKILSNGYKTCVITPFGDQFKKMRKVVMTELVCPAR 169

Query: 149 LHRMLGHRNAEADTLLRYIF---RGGSVVNVRSITQHYCGNIMRRMLFNQRYYGKG-RDD 208
              +   R+ E D L  +++   +    V+ R +T+HYCGN +++++F  R + K    D
Sbjct: 170 HRWLHQKRSEENDHLTAWVYNMVKNSGSVDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPD 229

Query: 209 GGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPFDLDGHEKVTKKALNVVQKYDEPII 268
           GGPT E+ EH +A+   +    AF ISD++P L   DL+GHEK+ +++  ++ KY +PII
Sbjct: 230 GGPTVEDVEHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPII 289

Query: 269 DERVQRWKDEKLEEVKDNILDILILLQGENAKPLLSVAEIKAQIMELQLATIDNPSNAVE 328
           DER++ W++ K  +++D  LDI I ++ E   PLL+  EIK  I EL +A  DNPSNAVE
Sbjct: 290 DERIKMWREGKRTQIED-FLDIFISIKDEQGNPLLTADEIKPTIKELVMAAPDNPSNAVE 349

Query: 329 WAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIPKLKYLTACIRESFRLHPFSPFNVP 388
           WAMAE+VN+P+IL KA+EE+D+VVG+ERLV+E DIPKL Y+ A +RE+FRLHP + FN+P
Sbjct: 350 WAMAEMVNKPEILRKAMEEIDRVVGKERLVQESDIPKLNYVKAILREAFRLHPVAAFNLP 409

Query: 389 HVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNPERHMNDRTRELGLLEPDL 448
           HV+ +D  VAGY IPKGS+VLLSR GLGRNP++W DP+ F PERH+N+   E+ L E DL
Sbjct: 410 HVALSDTTVAGYHIPKGSQVLLSRYGLGRNPKVWADPLCFKPERHLNE-CSEVTLTENDL 469

Query: 449 RFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWTLLPGITKIDFFQ-AEELSLPKPLH 508
           RF+SF  G+RGC   +LGT +T M+ ARLL GF+W L    T+++  + + ++ L KPL 
Sbjct: 470 RFISFSTGKRGCAAPALGTALTTMMLARLLQGFTWKLPENETRVELMESSHDMFLAKPLV 529

Query: 509 LHAQPRLSHIMYP 517
           +    RL   +YP
Sbjct: 530 MVGDLRLPEHLYP 538

BLAST of CmaCh10G009000 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 435.6 bits (1119), Expect = 5.4e-122
Identity = 217/501 (43.31%), Postives = 320/501 (63.87%), Query Frame = 0

Query: 28  SNGKAAELPPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIACIRLGSNYIIPVA 87
           S+ +   LPPGPK WPLVG LP +L  N P ++WIH++M++ NT+IACIRL + ++IPV 
Sbjct: 35  SHTRNLSLPPGPKSWPLVGNLPEILGRNKPVFRWIHSLMEELNTDIACIRLANTHVIPVT 94

Query: 88  SQEIALEFLKTYDAIFASRPPVSNAVDILNGRCVTTIFAPMGEQWRKMRRILALEVLNPS 147
           S  IA E LK  D++FA R P++   +  +   +T      GEQW+KMRR++A  V +  
Sbjct: 95  SPRIAREILKKQDSVFAIR-PLTMGTEYCSRGYLTIAVQSQGEQWKKMRRVVASHVTSKK 154

Query: 148 TLHRMLGHRNAEADTLLRYI----------FRGGSVVNVRSITQHYCGNIMRRMLFNQRY 207
           +   ML  R  EAD L+RYI            G +V+++R + + Y GN+ R+M+F  R+
Sbjct: 155 SFKLMLEKRTEEADNLVRYINNRCVKNRGNGNGLAVIDLRFVVRQYSGNVARKMMFGIRH 214

Query: 208 YGKG-RDDGGPTFEEEEHDQALLTIIRHIHAFSISDFMPWLKPFDLDGHEKVTKKALNVV 267
           +GKG  D+ GP  EE EH ++L T++ H++AF++SD++PWL+  DL+GHEKV   A+  V
Sbjct: 215 FGKGSEDESGPGSEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVADAMRNV 274

Query: 268 QKYDEPIIDERVQRWKDEKLEEVKDNILDILILLQGENAKPLLSVAEIKAQIMELQLATI 327
            KY++P +DER+ +W++ K++E +D  LD+ I+ +  N KP LS  EIKAQ+ EL LAT+
Sbjct: 275 SKYNDPFVDERLMQWRNGKMKEPQD-FLDMFIMAKDTNGKPTLSEEEIKAQVTELMLATV 334

Query: 328 DNPSNAVEWAMAELVNQPKILEKAIEELDKVVGRERLVEEHDIPKLKYLTACIRESFRLH 387
           DNPSNA EWAMAE++N+P I++KA+EE+D+   RE + E               E+FRLH
Sbjct: 335 DNPSNAAEWAMAEMINEPSIMQKAVEEIDRSNLRESVCE---------------ETFRLH 394

Query: 388 PFSPFNVPHVSNTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNPERHMNDRTRE 447
           P +PFN+PH+S  D +V GYFIPKGS +L+SR G+GRNP +W+ P++FNPERH+ +    
Sbjct: 395 PVAPFNLPHMSTADAVVDGYFIPKGSHMLISRMGIGRNPNVWDKPLKFNPERHLGN---- 454

Query: 448 LGLLEPDLRFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWTLLPGITKIDFFQAE-E 507
                                     +++T ML ARL+ GF+W+ +PG +KID  +++ +
Sbjct: 455 -------------------------NSSMTYMLLARLIQGFTWSPVPGESKIDISESKSD 489

Query: 508 LSLPKPLHLHAQPRLSHIMYP 517
           L + KPLH  A PRL   +YP
Sbjct: 515 LFMAKPLHAVATPRLDPHVYP 489

BLAST of CmaCh10G009000 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 412.9 bits (1060), Expect = 3.8e-115
Identity = 203/494 (41.09%), Postives = 330/494 (66.80%), Query Frame = 0

Query: 34  ELPPGPKPWPLVGCLPTMLATNLPTYKWIHAVMKQFNTEIACIRLGSNYIIPVASQEIAL 93
           +LPPGP+ WP+VG +  M+  N P + WIH VM++  T+IAC R    ++I V S +IA 
Sbjct: 36  QLPPGPRGWPIVGNMLQMI-INRPAHLWIHRVMEELQTDIACYRFARFHVITVTSSKIAR 95

Query: 94  EFLKTYDAIFASRPPVSNAVDILNGRCVTTIFAPMGEQWRKMRRILALEVLNPSTLHRML 153
           E L+  D + A R   S A  +++       F+  GE W+ +++++  ++++P+TL + L
Sbjct: 96  EVLREKDEVLADRSE-SYASHLISHGYKNISFSSYGENWKLVKKVMTTKLMSPTTLSKTL 155

Query: 154 GHRNAEADTLLRYIF---RGGSV---VNVRSITQHYCGNIMRRMLFNQRYYGKGRDDGGP 213
           G+RN EAD ++ Y++   + GSV   +NVR     YC  +M RM+F QR++ +  ++GG 
Sbjct: 156 GYRNIEADNIVTYVYNLCQLGSVRKPINVRDTILTYCHAVMMRMMFGQRHFDEVVENGGL 215

Query: 214 TFEEEEHDQALLTIIRHIHAFSISDFMPWLKPFDLDGHEKVTKKALNVVQKYDEPIIDER 273
             +E+EH  A+   +    +F++++++P+L+ +++D  E   ++A++++   ++PII ER
Sbjct: 216 GPKEKEHMDAIYLALDCFFSFNLTNYIPFLRGWNVDKAETEVREAVHIINICNDPIIQER 275

Query: 274 VQRWKDEKLEEVKDNILDILILLQGENAKPLLSVAEIKAQIMELQLATIDNPSNAVEWAM 333
           +  W+ +  ++++++ LDILI L+ +    L +  EI+AQ  E+ LATIDN  N VEW +
Sbjct: 276 IHLWRKKGGKQMEEDWLDILITLKDDQGMHLFTFDEIRAQCKEINLATIDNTMNNVEWTI 335

Query: 334 AELVNQPKILEKAIEELDKVVGRERLVEEHDIPKLKYLTACIRESFRLHPFSPFNVPHVS 393
           AE++N P+ILEKA  ELD +VG++RLV+E DI +L Y+ AC +ESFRLHP + F   HV+
Sbjct: 336 AEMLNHPEILEKATNELDIIVGKDRLVQESDISQLNYIKACSKESFRLHPANVFMPHHVA 395

Query: 394 NTDIIVAGYFIPKGSEVLLSRTGLGRNPRIWEDPMRFNPERHMN---DRTRELGLLEPDL 453
             D  +AGYF+PKGS++L+SR GLGRNP+IW++P  F PER+++   +++  + L+EPD+
Sbjct: 396 REDTTLAGYFVPKGSQILVSRLGLGRNPKIWDEPNAFKPERYLDGHVEKSLGVTLMEPDM 455

Query: 454 RFMSFGRGRRGCPGSSLGTNITMMLFARLLHGFSWTLLPGITKIDFFQAE-ELSLPKPLH 513
           RF++FG GRR CPG+ +GT++T+ML ARL+ GF WTL  G + ++   AE  L + KPL 
Sbjct: 456 RFVTFGTGRRSCPGTKIGTSMTIMLLARLIQGFEWTLPIGKSSVELISAESNLFMAKPLL 515

Query: 514 LHAQPRLSHIMYPK 518
             A+PRL+  +YPK
Sbjct: 516 ACAKPRLAPSLYPK 527