CmaCh10G008210 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh10G008210
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	(1->3)-beta-glucan endohydrolase
Location	Cma_Chr10: 3830838 .. 3832190 (-)
RNA-Seq Expression	CmaCh10G008210
Synteny	CmaCh10G008210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATGTTGAATAGTGTAAAACGTTTAACTGTGGCATTGTAGCAGTTTCTTCGGATCTCTCTCTCTCTCTCTCGTAAATGTTTGAGCTTTATCCATTATGTGCATACCCATGTGACAATCGAGTCATGTTTGAACTTTAAAACGTTATTCGATTTGATTTTAAACTTCAGATCAATAGGTATACAGAACAGTGTAAAGCATTTACTGTGTCGTTGTAGCAGTTTCATCGAATAATTGATATCTTATCTATCTGCTGAGCTACCCTCTTACTCAATTTCATGTAGGTGCTCAAATTGGTGTGTGTTATGGACAACTCGGGAACGACCTACCATCTCCCGTGGAAGTTATCGAGCTTTACAAACAAAACAACATCCAAAGAATGCGACTTTACGCTCCAGACCATCCTACTTTCGACGCTCTACGAGGCTCGAATATCGAGCTCATGCTTGGAATTCCGAACAACGAGCTCGAAGATCTAGCCAACTTCCAAGAAAAAGCTGATTCATGGGTTCATGACAACCTCGTAAGCTTTGGTGACGTAAAATTCAAGTACATTGTGGTTGGAAACGAAATAAAAACAAACGACCAAGCAGCTAGTTTCCTCGTACCAGCCATGCAAAATATCCAAAATGCCATTTCCGCTGTGGGTTTAGGAGGCCAAATCAAGGTATCAACCGCATTCGACACTGGAATTCTTGCAGAATCCTACCCTCCTTCAAAGGGTTCCTTCAAAACCGACTATCTTCCGATCCTTAACCCGACCATTCGCTTCCTTCTCGACAATAACGCCCCGTTGCTCGTAAACGTGTACCCGTACTTCAGTTACGTCGCAAACACGGCCGATATTTCTATGGATTATGCACTTTTTAGAGGAACATCTCCATCTGTTGAAGATGGCCAATTTGTTTACCATAATTTGTTTGATGCCATTTTGGATGCTGTTTATTCTGCTTTGGAGAAGGCGGGCAGCGACGGGCTGGAAATTGCTGTATCGGAGACCGGTTGGCCGACGGAGGGCGGCGAGGCGGCGACGGTGGAGAATGCAAACACGTACAACAACAATTTGATTCAACATGTGAAAGAAGGGACACCCAAGAGAAGTGGAAAGCCTGTGGAAACCTATGTGTTTGCTATGTTTGATGAGAATGAGAAGACAACGCCACCGGAGGTTGAGAAACATTGGGGTTTGTTCTCTCCAAGCAAGATGCCCAAGTACGAGGTTAACTTCAACTAAATTCCTACGGATCTATGTCATATCTTTGTCGGAATCTTTTATTTAATAATATTAAAATCTTCCGTTATGTATTCAAATACATGACATGGTTCATGTGTTCAAGCCAAGATAAGAAA

mRNA sequence

ATGGGTGCTCAAATTGGTGTGTGTTATGGACAACTCGGGAACGACCTACCATCTCCCGTGGAAGTTATCGAGCTTTACAAACAAAACAACATCCAAAGAATGCGACTTTACGCTCCAGACCATCCTACTTTCGACGCTCTACGAGGCTCGAATATCGAGCTCATGCTTGGAATTCCGAACAACGAGCTCGAAGATCTAGCCAACTTCCAAGAAAAAGCTGATTCATGGGTTCATGACAACCTCGTAAGCTTTGGTGACGTAAAATTCAAGTACATTGTGGTTGGAAACGAAATAAAAACAAACGACCAAGCAGCTAGTTTCCTCGTACCAGCCATGCAAAATATCCAAAATGCCATTTCCGCTGTGGGTTTAGGAGGCCAAATCAAGGTATCAACCGCATTCGACACTGGAATTCTTGCAGAATCCTACCCTCCTTCAAAGGGTTCCTTCAAAACCGACTATCTTCCGATCCTTAACCCGACCATTCGCTTCCTTCTCGACAATAACGCCCCGTTGCTCGTAAACGTGTACCCGTACTTCAGTTACGTCGCAAACACGGCCGATATTTCTATGGATTATGCACTTTTTAGAGGAACATCTCCATCTGTTGAAGATGGCCAATTTGTTTACCATAATTTGTTTGATGCCATTTTGGATGCTGTTTATTCTGCTTTGGAGAAGGCGGGCAGCGACGGGCTGGAAATTGCTGTATCGGAGACCGGTTGGCCGACGGAGGGCGGCGAGGCGGCGACGGTGGAGAATGCAAACACGTACAACAACAATTTGATTCAACATGTGAAAGAAGGGACACCCAAGAGAAGTGGAAAGCCTGTGGAAACCTATGTGTTTGCTATGTTTGATGAGAATGAGAAGACAACGCCACCGGAGGTTGAGAAACATTGGGGTTTGTTCTCTCCAAGCAAGATGCCCAAGTACGAGGTTAACTTCAACTAAATTCCTACGGATCTATGTCATATCTTTGTCGGAATCTTTTATTTAATAATATTAAAATCTTCCGTTATGTATTCAAATACATGACATGGTTCATGTGTTCAAGCCAAGATAAGAAA

Coding sequence (CDS)

ATGGGTGCTCAAATTGGTGTGTGTTATGGACAACTCGGGAACGACCTACCATCTCCCGTGGAAGTTATCGAGCTTTACAAACAAAACAACATCCAAAGAATGCGACTTTACGCTCCAGACCATCCTACTTTCGACGCTCTACGAGGCTCGAATATCGAGCTCATGCTTGGAATTCCGAACAACGAGCTCGAAGATCTAGCCAACTTCCAAGAAAAAGCTGATTCATGGGTTCATGACAACCTCGTAAGCTTTGGTGACGTAAAATTCAAGTACATTGTGGTTGGAAACGAAATAAAAACAAACGACCAAGCAGCTAGTTTCCTCGTACCAGCCATGCAAAATATCCAAAATGCCATTTCCGCTGTGGGTTTAGGAGGCCAAATCAAGGTATCAACCGCATTCGACACTGGAATTCTTGCAGAATCCTACCCTCCTTCAAAGGGTTCCTTCAAAACCGACTATCTTCCGATCCTTAACCCGACCATTCGCTTCCTTCTCGACAATAACGCCCCGTTGCTCGTAAACGTGTACCCGTACTTCAGTTACGTCGCAAACACGGCCGATATTTCTATGGATTATGCACTTTTTAGAGGAACATCTCCATCTGTTGAAGATGGCCAATTTGTTTACCATAATTTGTTTGATGCCATTTTGGATGCTGTTTATTCTGCTTTGGAGAAGGCGGGCAGCGACGGGCTGGAAATTGCTGTATCGGAGACCGGTTGGCCGACGGAGGGCGGCGAGGCGGCGACGGTGGAGAATGCAAACACGTACAACAACAATTTGATTCAACATGTGAAAGAAGGGACACCCAAGAGAAGTGGAAAGCCTGTGGAAACCTATGTGTTTGCTATGTTTGATGAGAATGAGAAGACAACGCCACCGGAGGTTGAGAAACATTGGGGTTTGTTCTCTCCAAGCAAGATGCCCAAGTACGAGGTTAACTTCAACTAA

Protein sequence

MGAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWGLFSPSKMPKYEVNFN

Homology

[Close]

BLAST of CmaCh10G008210 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 8.0e-113
Identity = 192/316 (60.76%), Postives = 245/316 (77.53%), Query Frame = 0


Query: 2   GAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNN 61
           GA IGVC G +G+DLP   EV+ LYK NNI RMRLY P+    +ALRGSNI+L+LG+PN 
Sbjct: 37  GAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLLLGVPNE 96

Query: 62  ELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISA 121
            L+ +A  Q  A++WV +N+ ++ +VKFKYI VGNE+K +D  A FLVPAM+NIQ AIS 
Sbjct: 97  NLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNEVKPSDSFAQFLVPAMRNIQEAISL 156

Query: 122 VGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFS 181
            GL  +IKVSTA DTG+L E++PPS GSFK++Y  +L P IRFL+ + +PLLVN+YPYF+
Sbjct: 157 AGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVNLYPYFA 216

Query: 182 YVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETG 241
           Y  NT DI +DYALF   S  V+DG F Y NLFDA+LD VY+ALEKAG   L++ +SETG
Sbjct: 217 YSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKVVISETG 276

Query: 242 WPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHW 301
           WP+  G A T++NA T+ +NLIQHVKEGTP+R G+P+ETY+FAMFDEN KT  PE+EKHW
Sbjct: 277 WPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGRPIETYIFAMFDENRKT--PELEKHW 336

Query: 302 GLFSPSKMPKYEVNFN 318
           GLFSP+K PKY+++FN
Sbjct: 337 GLFSPTKQPKYQISFN 350

[Close]

BLAST of CmaCh10G008210 vs. ExPASy Swiss-Prot
Match: Q9M2M0 (Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN=BG1 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.3e-110
Identity = 188/316 (59.49%), Postives = 240/316 (75.95%), Query Frame = 0


Query: 2   GAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNN 61
           G Q+GVCYG+ GN+LPSP E I L+KQ NIQR+RLY+PDH    ALRGSNIE+ LG+PN+
Sbjct: 24  GGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNS 83

Query: 62  ELEDLANFQEKADSWVHDNLVSFGD-VKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAIS 121
            L+ +A+ Q +A++WV   ++++ + V+F+YI VGNE+K +D  A FLVPAM+NI  A+ 
Sbjct: 84  YLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVL 143

Query: 122 AVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYF 181
           A GLGG+IKVST+ D G+L ESYPPSKGSF+ D + ++ P IRFL+  N+PLL+N+Y YF
Sbjct: 144 AAGLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYF 203

Query: 182 SYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSET 241
           SY  N   I +DYALF   S  V D    Y NLFDA+LDA+YSALEK+G   LEI V+ET
Sbjct: 204 SYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAET 263

Query: 242 GWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKH 301
           GWPT GG    +ENA  YNNNLI+HVK GTPKR GK +ETY+FA++DEN+K TPP VEK 
Sbjct: 264 GWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKF 323

Query: 302 WGLFSPSKMPKYEVNF 317
           WGLF P+K PKY++NF
Sbjct: 324 WGLFYPNKQPKYDINF 339

[Close]

BLAST of CmaCh10G008210 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.3e-110
Identity = 197/318 (61.95%), Postives = 245/318 (77.04%), Query Frame = 0


Query: 3   AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 62
           AQ GVCYG+LGN+LP+P EV+ LY Q NI+RMR+Y P     +ALRGSNIEL+L IPN+ 
Sbjct: 32  AQSGVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEVLEALRGSNIELLLDIPNDN 91

Query: 63  LEDLANFQEKADSWVHDNLVSF-GDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISA 122
           L +LA+ Q+ A+ WV DN+ ++  +V+F+Y+ VGNE+K     A FLVPA++NIQ AIS 
Sbjct: 92  LRNLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEHSFAQFLVPALENIQRAISN 151

Query: 123 VGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYL-PILNPTIRFLLDNNAPLLVNVYPYF 182
            GLG Q+KVSTA DTG LAES+PPSKGSFK+DY    L+  IRFL++NNAPL+VNVY YF
Sbjct: 152 AGLGNQVKVSTAIDTGALAESFPPSKGSFKSDYRGAYLDGVIRFLVNNNAPLMVNVYSYF 211

Query: 183 SYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSET 242
           +Y AN  DIS+DYALFR  S  V+DG   Y NLFDA +DAVY+ALEKAG   L I VSE+
Sbjct: 212 AYTANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDAVYAALEKAGGGSLNIVVSES 271

Query: 243 GWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKH 302
           GWP+ GG A +++NA TYN NL+++VK+GTPKR G P+ETYVFAMFDEN+K   PE EK 
Sbjct: 272 GWPSSGGTATSLDNARTYNTNLVRNVKQGTPKRPGAPLETYVFAMFDENQK--QPEFEKF 331

Query: 303 WGLFSP-SKMPKYEVNFN 318
           WGLFSP +K PKY +NFN
Sbjct: 332 WGLFSPITKQPKYSINFN 347

[Close]

BLAST of CmaCh10G008210 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.7e-102
Identity = 184/320 (57.50%), Postives = 235/320 (73.44%), Query Frame = 0


Query: 2   GAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNN 61
           GAQ GVCYG+ GN LPSP +V+ L  +NNI+RMR+Y PD PT +ALRGSNIELMLG+PN 
Sbjct: 23  GAQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDPDQPTLEALRGSNIELMLGVPNP 82

Query: 62  ELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEI---KTNDQAASFLVPAMQNIQNA 121
           +LE++A  Q  AD+WV +N+ ++G+VKF+YI VGNE+     N +    L+ AM+NIQ A
Sbjct: 83  DLENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLNENSKYVPVLLNAMRNIQTA 142

Query: 122 ISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYP 181
           IS  GLG QIKVSTA +TG+  ++ PPS G FK D    + P I FL+ N APLLVN+YP
Sbjct: 143 ISGAGLGNQIKVSTAIETGLTTDTSPPSNGRFKDDVRQFIEPIINFLVTNRAPLLVNLYP 202

Query: 182 YFSYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVS 241
           YF+ +AN ADI ++YALF  +   V D    Y NLFDAILDA YSALEKA    LEI VS
Sbjct: 203 YFA-IANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDATYSALEKASGSSLEIVVS 262

Query: 242 ETGWPTEG-GEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEV 301
           E+GWP+ G G+  +++NA TYNNNLI HVK G+PKR   P+ETYVFA+FDE++K   PE+
Sbjct: 263 ESGWPSAGAGQLTSIDNARTYNNNLISHVKGGSPKRPSGPIETYVFALFDEDQK--DPEI 322

Query: 302 EKHWGLFSPSKMPKYEVNFN 318
           EKH+GLFS +  PKY+++FN
Sbjct: 323 EKHFGLFSANMQPKYQISFN 339

[Close]

BLAST of CmaCh10G008210 vs. ExPASy Swiss-Prot
Match: P33157 (Glucan endo-1,3-beta-glucosidase, acidic isoform OS=Arabidopsis thaliana OX=3702 GN=BG2 PE=1 SV=2)

HSP 1 Score: 360.5 bits (924), Expect = 1.9e-98
Identity = 182/315 (57.78%), Postives = 227/315 (72.06%), Query Frame = 0


Query: 4   QIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNEL 63
           QIGVCYG LG+ LPSP +V+ LYKQ NIQRMRLY PD     ALRGS+IEL+L +P+++L
Sbjct: 31  QIGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAALRGSDIELILDVPSSDL 90

Query: 64  EDLANFQEKADSWVHDNLVSFGD-VKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 123
           E LA+ Q +AD WV +N+ S+ D V+F+YI VGNE+K +     FL+ AMQNI+NA+S  
Sbjct: 91  ERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPS--VGGFLLQAMQNIENAVSGA 150

Query: 124 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 183
           GL  ++KVSTA  T    ++ PPS+G F+ +Y   L P I FL    +PLLVN+YPYFSY
Sbjct: 151 GL--EVKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNLYPYFSY 210

Query: 184 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 243
           + +TA+I +DYALF   S    D  + Y NLFDA LD+VY+ALEK+G   LEI VSETGW
Sbjct: 211 MGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSVYAALEKSGGGSLEIVVSETGW 270

Query: 244 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 303
           PTEG    +VENA TY NNLIQHVK G+P+R GK +ETY+FAMFDEN+K   P  EK WG
Sbjct: 271 PTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIFAMFDENKK--EPTYEKFWG 330

Query: 304 LFSPSKMPKYEVNFN 318
           LF P +  KYEVNFN
Sbjct: 331 LFHPDRQSKYEVNFN 339

[Close]

BLAST of CmaCh10G008210 vs. ExPASy TrEMBL
Match: A0A6J1JIH6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.6e-180
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 3   AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 62
           AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE
Sbjct: 31  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 90

Query: 63  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 122
           LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV
Sbjct: 91  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 150

Query: 123 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 182
           GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY
Sbjct: 151 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 210

Query: 183 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 242
           VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW
Sbjct: 211 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 270

Query: 243 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 302
           PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG
Sbjct: 271 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 330

Query: 303 LFSPSKMPKYEVNFN 318
           LFSPSKMPKYEVNFN
Sbjct: 331 LFSPSKMPKYEVNFN 345

[Close]

BLAST of CmaCh10G008210 vs. ExPASy TrEMBL
Match: A0A6J1HCY3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.0e-175
Identity = 308/315 (97.78%), Postives = 309/315 (98.10%), Query Frame = 0


Query: 3   AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 62
           AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYA +HPTFDALRGSNIELMLGIPNNE
Sbjct: 31  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYALNHPTFDALRGSNIELMLGIPNNE 90

Query: 63  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 122
           LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKT DQAASFLVPAMQNIQNAISAV
Sbjct: 91  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTYDQAASFLVPAMQNIQNAISAV 150

Query: 123 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 182
           GL GQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY
Sbjct: 151 GLEGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 210

Query: 183 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 242
           VANTADISMDYALFRG SPSVEDGQFVYHNLFDAILDAVYSALEKAG DGLEI VSETGW
Sbjct: 211 VANTADISMDYALFRGASPSVEDGQFVYHNLFDAILDAVYSALEKAGGDGLEIVVSETGW 270

Query: 243 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 302
           PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG
Sbjct: 271 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 330

Query: 303 LFSPSKMPKYEVNFN 318
           LFSPSKMPKYEVNFN
Sbjct: 331 LFSPSKMPKYEVNFN 345

[Close]

BLAST of CmaCh10G008210 vs. ExPASy TrEMBL
Match: A0A6J1CBL7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 7.2e-141
Identity = 247/315 (78.41%), Postives = 280/315 (88.89%), Query Frame = 0


Query: 3   AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 62
           AQIGVCYGQLG+DLPSPVEVI+LY QNNI+RMRLYAP+ P+ +ALRGSNIELMLGIPN+E
Sbjct: 32  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 91

Query: 63  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 122
           LE +A  QE A+SW  DN++SFGDVKFKYIVVGNEIK +D AA FLVPAMQNIQNAISA 
Sbjct: 92  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 151

Query: 123 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 182
           GL G+IKVSTA DTG+LAESYPPSKGSF++DYLPIL+PTIRFL+DNN+PLL+N+Y YFS+
Sbjct: 152 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 211

Query: 183 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 242
           ++NT DIS+DYALF G S  V DGQ VY NLFDAILDAVYSALEK+   GLEI VSE+GW
Sbjct: 212 ISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 271

Query: 243 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 302
           PT+GGEAA V+NA TYNNNLIQHVK GTPKR G P+ETYVFAMFDEN+KTTPPEVEKHWG
Sbjct: 272 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 331

Query: 303 LFSPSKMPKYEVNFN 318
           LFSP+K PKY VNF+
Sbjct: 332 LFSPNKQPKYLVNFH 345

[Close]

BLAST of CmaCh10G008210 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.6e-135
Identity = 239/311 (76.85%), Postives = 275/311 (88.42%), Query Frame = 0


Query: 2   GAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNN 61
           GAQIGVCYGQLGNDLPSP EVI+LY QNNIQRMRLYAP+  TF+ALRGS+IELMLG+PN+
Sbjct: 32  GAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQDTFNALRGSSIELMLGLPND 91

Query: 62  ELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISA 121
           +++ +A  Q+ A++W+ DN+++F DV FKYIVVGNEIKTN++AA FLVPAMQNIQNAISA
Sbjct: 92  QIQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISA 151

Query: 122 VGLGGQIKVSTAFDTGIL-AESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYF 181
           VGL GQIKVSTAF TGIL AES+PPS GSF  +YLPILNPTIRFLLDNN+PLL+N+YPYF
Sbjct: 152 VGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNPTIRFLLDNNSPLLLNLYPYF 211

Query: 182 SYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSET 241
           SYVA T ++ +DYA+F GTS  VEDG+F Y NLFDAILD VYSALEK G   LE+ VSET
Sbjct: 212 SYVA-TPNMELDYAIFTGTS-LVEDGEFNYQNLFDAILDTVYSALEKNGGGSLEVVVSET 271

Query: 242 GWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKH 301
           GWPTEGGEAATV+NA TYNNNLIQHVK+GTPKR G+ +ETYVFAMFDENEKTTPPEVE+H
Sbjct: 272 GWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERH 331

Query: 302 WGLFSPSKMPK 312
           WGLFSP+K PK
Sbjct: 332 WGLFSPNKQPK 340

[Close]

BLAST of CmaCh10G008210 vs. ExPASy TrEMBL
Match: A0A1S3C9Z3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.4e-133
Identity = 238/316 (75.32%), Postives = 273/316 (86.39%), Query Frame = 0


Query: 3   AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 62
           AQIGVCYGQLGNDLPSP EVI+LY QNNIQRMRLYAP+  TF+ALRGS+IELMLG+PN+E
Sbjct: 33  AQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQATFNALRGSSIELMLGLPNDE 92

Query: 63  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 122
           ++ +A  Q+ A++WV DN+++F DV FKYIVVGNEIKTN++AA FLVPAMQNIQNAISAV
Sbjct: 93  IQSMAATQDNANAWVQDNILNFPDVNFKYIVVGNEIKTNEEAARFLVPAMQNIQNAISAV 152

Query: 123 GLGGQIKVSTAFDTGILA-ESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFS 182
           GLGG+IKVSTAF TGIL+ ES+PPS GSF   YLPILNPTIRFLLDNN+PLLVN+YPYFS
Sbjct: 153 GLGGKIKVSTAFHTGILSVESFPPSHGSFDASYLPILNPTIRFLLDNNSPLLVNLYPYFS 212

Query: 183 YVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETG 242
           YV  T  + +DYA+F GTS  V DG+F Y NLF AILD VYSALEK G   LEI VSETG
Sbjct: 213 YV-GTPSMELDYAIFTGTS-LVVDGEFNYQNLFHAILDTVYSALEKNGGGSLEIVVSETG 272

Query: 243 WPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHW 302
           WPT+GGEAATV+NA TY NNLIQ VK+GTPKR G+ +ETYVFAMFDENEKTTPPEVE+HW
Sbjct: 273 WPTDGGEAATVDNARTYTNNLIQRVKQGTPKRQGRAIETYVFAMFDENEKTTPPEVERHW 332

Query: 303 GLFSPSKMPKYEVNFN 318
           GLFSP+K PK+ V+FN
Sbjct: 333 GLFSPNKQPKFSVSFN 346

[Close]

BLAST of CmaCh10G008210 vs. NCBI nr
Match: XP_022987103.1 (probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima])

HSP 1 Score: 641.7 bits (1654), Expect = 3.3e-180
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 3   AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 62
           AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE
Sbjct: 31  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 90

Query: 63  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 122
           LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV
Sbjct: 91  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 150

Query: 123 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 182
           GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY
Sbjct: 151 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 210

Query: 183 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 242
           VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW
Sbjct: 211 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 270

Query: 243 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 302
           PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG
Sbjct: 271 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 330

Query: 303 LFSPSKMPKYEVNFN 318
           LFSPSKMPKYEVNFN
Sbjct: 331 LFSPSKMPKYEVNFN 345

[Close]

BLAST of CmaCh10G008210 vs. NCBI nr
Match: XP_022961079.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata])

HSP 1 Score: 624.8 bits (1610), Expect = 4.1e-175
Identity = 308/315 (97.78%), Postives = 309/315 (98.10%), Query Frame = 0


Query: 3   AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 62
           AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYA +HPTFDALRGSNIELMLGIPNNE
Sbjct: 31  AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYALNHPTFDALRGSNIELMLGIPNNE 90

Query: 63  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 122
           LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKT DQAASFLVPAMQNIQNAISAV
Sbjct: 91  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTYDQAASFLVPAMQNIQNAISAV 150

Query: 123 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 182
           GL GQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY
Sbjct: 151 GLEGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 210

Query: 183 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 242
           VANTADISMDYALFRG SPSVEDGQFVYHNLFDAILDAVYSALEKAG DGLEI VSETGW
Sbjct: 211 VANTADISMDYALFRGASPSVEDGQFVYHNLFDAILDAVYSALEKAGGDGLEIVVSETGW 270

Query: 243 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 302
           PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG
Sbjct: 271 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 330

Query: 303 LFSPSKMPKYEVNFN 318
           LFSPSKMPKYEVNFN
Sbjct: 331 LFSPSKMPKYEVNFN 345

[Close]

BLAST of CmaCh10G008210 vs. NCBI nr
Match: KAG6590085.1 (Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 621.7 bits (1602), Expect = 3.5e-174
Identity = 305/317 (96.21%), Postives = 310/317 (97.79%), Query Frame = 0


Query: 1   MGAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPN 60
           +GAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAP+HPTFDALRGSNIELMLGIPN
Sbjct: 76  IGAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPNHPTFDALRGSNIELMLGIPN 135

Query: 61  NELEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAIS 120
           NELEDLANFQEKADSWV DNLVSFGDVKFKYIVVGNEIKT DQAASFLVPAMQNIQNAIS
Sbjct: 136 NELEDLANFQEKADSWVQDNLVSFGDVKFKYIVVGNEIKTYDQAASFLVPAMQNIQNAIS 195

Query: 121 AVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYF 180
           AVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYF
Sbjct: 196 AVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYF 255

Query: 181 SYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSET 240
           SYVANTADISMDYALFRGTSPSV DGQFVYHNLFDAILDAVYSALE +G D +EI VSET
Sbjct: 256 SYVANTADISMDYALFRGTSPSVVDGQFVYHNLFDAILDAVYSALETSGGDRVEIVVSET 315

Query: 241 GWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKH 300
           GWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKH
Sbjct: 316 GWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKH 375

Query: 301 WGLFSPSKMPKYEVNFN 318
           WGLFSPSK+PKYEVNFN
Sbjct: 376 WGLFSPSKLPKYEVNFN 392

[Close]

BLAST of CmaCh10G008210 vs. NCBI nr
Match: XP_023516024.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 556.6 bits (1433), Expect = 1.4e-154
Identity = 273/284 (96.13%), Postives = 277/284 (97.54%), Query Frame = 0


Query: 34  MRLYAPDHPTFDALRGSNIELMLGIPNNELEDLANFQEKADSWVHDNLVSFGDVKFKYIV 93
           MRLYAP+HPTFDALRGSNIELMLGIPNNELEDLANFQEKADSWV DNLVSFGDVKFKYIV
Sbjct: 1   MRLYAPNHPTFDALRGSNIELMLGIPNNELEDLANFQEKADSWVQDNLVSFGDVKFKYIV 60

Query: 94  VGNEIKTNDQAASFLVPAMQNIQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTD 153
           VGNEIKT DQAASFLVPAMQNIQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTD
Sbjct: 61  VGNEIKTYDQAASFLVPAMQNIQNAISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTD 120

Query: 154 YLPILNPTIRFLLDNNAPLLVNVYPYFSYVANTADISMDYALFRGTSPSVEDGQFVYHNL 213
           YLPILNPTIRFLLDNNAPLLVNVYPYFSYVANT DISMDYALFRGTSPSV DGQFVYHNL
Sbjct: 121 YLPILNPTIRFLLDNNAPLLVNVYPYFSYVANTVDISMDYALFRGTSPSVVDGQFVYHNL 180

Query: 214 FDAILDAVYSALEKAGSDGLEIAVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKR 273
           FDAILDAVYSALE +G DG+EI VSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKR
Sbjct: 181 FDAILDAVYSALETSGGDGVEIVVSETGWPTEGGEAATVENANTYNNNLIQHVKEGTPKR 240

Query: 274 SGKPVETYVFAMFDENEKTTPPEVEKHWGLFSPSKMPKYEVNFN 318
           SGKPVETYVFAMFDENEKTTPPEVEKHWGLFSPSK+PKYEVNFN
Sbjct: 241 SGKPVETYVFAMFDENEKTTPPEVEKHWGLFSPSKLPKYEVNFN 284

[Close]

BLAST of CmaCh10G008210 vs. NCBI nr
Match: XP_022139185.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 510.0 bits (1312), Expect = 1.5e-140
Identity = 247/315 (78.41%), Postives = 280/315 (88.89%), Query Frame = 0


Query: 3   AQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNE 62
           AQIGVCYGQLG+DLPSPVEVI+LY QNNI+RMRLYAP+ P+ +ALRGSNIELMLGIPN+E
Sbjct: 32  AQIGVCYGQLGDDLPSPVEVIDLYNQNNIERMRLYAPNQPSLNALRGSNIELMLGIPNHE 91

Query: 63  LEDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 122
           LE +A  QE A+SW  DN++SFGDVKFKYIVVGNEIK +D AA FLVPAMQNIQNAISA 
Sbjct: 92  LEAMATTQENANSWFQDNVLSFGDVKFKYIVVGNEIKPSDGAARFLVPAMQNIQNAISAA 151

Query: 123 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 182
           GL G+IKVSTA DTG+LAESYPPSKGSF++DYLPIL+PTIRFL+DNN+PLL+N+Y YFS+
Sbjct: 152 GLRGKIKVSTAIDTGVLAESYPPSKGSFRSDYLPILDPTIRFLVDNNSPLLLNLYTYFSH 211

Query: 183 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 242
           ++NT DIS+DYALF G S  V DGQ VY NLFDAILDAVYSALEK+   GLEI VSE+GW
Sbjct: 212 ISNTKDISLDYALFTGMS-MVSDGQLVYQNLFDAILDAVYSALEKSAGGGLEIVVSESGW 271

Query: 243 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 302
           PT+GGEAA V+NA TYNNNLIQHVK GTPKR G P+ETYVFAMFDEN+KTTPPEVEKHWG
Sbjct: 272 PTDGGEAAKVDNARTYNNNLIQHVKGGTPKRPGMPIETYVFAMFDENQKTTPPEVEKHWG 331

Query: 303 LFSPSKMPKYEVNFN 318
           LFSP+K PKY VNF+
Sbjct: 332 LFSPNKQPKYLVNFH 345

[Close]

BLAST of CmaCh10G008210 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 401.0 bits (1029), Expect = 9.0e-112
Identity = 188/316 (59.49%), Postives = 240/316 (75.95%), Query Frame = 0


Query: 2   GAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNN 61
           G Q+GVCYG+ GN+LPSP E I L+KQ NIQR+RLY+PDH    ALRGSNIE+ LG+PN+
Sbjct: 24  GGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNS 83

Query: 62  ELEDLANFQEKADSWVHDNLVSFGD-VKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAIS 121
            L+ +A+ Q +A++WV   ++++ + V+F+YI VGNE+K +D  A FLVPAM+NI  A+ 
Sbjct: 84  YLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVL 143

Query: 122 AVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYF 181
           A GLGG+IKVST+ D G+L ESYPPSKGSF+ D + ++ P IRFL+  N+PLL+N+Y YF
Sbjct: 144 AAGLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYF 203

Query: 182 SYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSET 241
           SY  N   I +DYALF   S  V D    Y NLFDA+LDA+YSALEK+G   LEI V+ET
Sbjct: 204 SYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAET 263

Query: 242 GWPTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKH 301
           GWPT GG    +ENA  YNNNLI+HVK GTPKR GK +ETY+FA++DEN+K TPP VEK 
Sbjct: 264 GWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDENQKPTPPYVEKF 323

Query: 302 WGLFSPSKMPKYEVNF 317
           WGLF P+K PKY++NF
Sbjct: 324 WGLFYPNKQPKYDINF 339

[Close]

BLAST of CmaCh10G008210 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 360.5 bits (924), Expect = 1.4e-99
Identity = 182/315 (57.78%), Postives = 227/315 (72.06%), Query Frame = 0


Query: 4   QIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNEL 63
           QIGVCYG LG+ LPSP +V+ LYKQ NIQRMRLY PD     ALRGS+IEL+L +P+++L
Sbjct: 31  QIGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGALAALRGSDIELILDVPSSDL 90

Query: 64  EDLANFQEKADSWVHDNLVSFGD-VKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAV 123
           E LA+ Q +AD WV +N+ S+ D V+F+YI VGNE+K +     FL+ AMQNI+NA+S  
Sbjct: 91  ERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKPS--VGGFLLQAMQNIENAVSGA 150

Query: 124 GLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 183
           GL  ++KVSTA  T    ++ PPS+G F+ +Y   L P I FL    +PLLVN+YPYFSY
Sbjct: 151 GL--EVKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIGFLASKQSPLLVNLYPYFSY 210

Query: 184 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 243
           + +TA+I +DYALF   S    D  + Y NLFDA LD+VY+ALEK+G   LEI VSETGW
Sbjct: 211 MGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSVYAALEKSGGGSLEIVVSETGW 270

Query: 244 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 303
           PTEG    +VENA TY NNLIQHVK G+P+R GK +ETY+FAMFDEN+K   P  EK WG
Sbjct: 271 PTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIFAMFDENKK--EPTYEKFWG 330

Query: 304 LFSPSKMPKYEVNFN 318
           LF P +  KYEVNFN
Sbjct: 331 LFHPDRQSKYEVNFN 339

[Close]

BLAST of CmaCh10G008210 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 355.1 bits (910), Expect = 5.7e-98
Identity = 181/315 (57.46%), Postives = 226/315 (71.75%), Query Frame = 0


Query: 4   QIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNNEL 63
           QIGVCYG+ GN+L    EV+ LY+Q NI+RMRLY P+  T +ALRGSNIEL+L +PN +L
Sbjct: 34  QIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNALRGSNIELVLDVPNPDL 93

Query: 64  EDLANFQEKADSWVHDNLVSFGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAISAVG 123
           + LA+ Q +AD+WV +N+ ++ +V F+YI VGNE++ +DQAASF++PAMQNI+ A+S++G
Sbjct: 94  QRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAASFVLPAMQNIERAVSSLG 153

Query: 124 LGGQIKVSTAFDT-GILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYFSY 183
               IKVSTA DT GI    +PPS G+F  ++   + P I FL    +PLLVN YPYFSY
Sbjct: 154 ----IKVSTAIDTRGI--SGFPPSSGTFTPEFRSFIAPVISFLSSKQSPLLVNNYPYFSY 213

Query: 184 VANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSETGW 243
             N  DI +DY LF   S  V DGQ  Y NLF AILD VY++LEKAG   LEI VSE+GW
Sbjct: 214 TGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLEKAGGGSLEIVVSESGW 273

Query: 244 PTEGGEAATVENANTYNNNLIQHVKEGTPKRSGKPVETYVFAMFDENEKTTPPEVEKHWG 303
           PT GG A  V+NA TY NNLIQ VK G+P+R G+  ETY+FAMFDEN K   PE EK WG
Sbjct: 274 PTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMFDENSK-QGPETEKFWG 333

Query: 304 LFSPSKMPKYEVNFN 318
           LF P+  PKY VNFN
Sbjct: 334 LFLPNLQPKYVVNFN 341

[Close]

BLAST of CmaCh10G008210 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 3.2e-85
Identity = 154/319 (48.28%), Postives = 217/319 (68.03%), Query Frame = 0


Query: 2   GAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNN 61
           G  +GVCYG +GN+LPS  + I L++QNNI+R+RLY P+    +ALR + IE+++G+PN 
Sbjct: 21  GESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGVPNT 80

Query: 62  ELEDLANFQEKADSWVHDNLVS-FGDVKFKYIVVGNEIKTNDQAASFLVPAMQNIQNAIS 121
           +L  L N    A SW+ +N+++ +  V FKYI VGNE+  ++     ++PAM+N+ +A+ 
Sbjct: 81  DLRSLTN-PSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSN-GGDVVLPAMRNVYDALR 140

Query: 122 AVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVYPYF 181
              L  +IKVSTA D  ++  S+PPS G F+ D    ++P I FL   N+ LL N+YPYF
Sbjct: 141 GANLQDRIKVSTAIDMTLIGNSFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIYPYF 200

Query: 182 SYVANTADISMDYALFRGTSPSVEDGQFVYHNLFDAILDAVYSALEKAGSDGLEIAVSET 241
           SYV N  DIS+ YALF   S  V DG   Y NLFDA+LD VYSA+E++G   L + VSE+
Sbjct: 201 SYVDNPRDISLSYALFTSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVVVSES 260

Query: 242 GWPTEGGEAATVENANTYNNNLIQHVKE--GTPKRSGKPVETYVFAMFDENEKTTPPEVE 301
           GWP+ GG AA+ +NA  +  NL   V+E  GTPKR G+ VETY+FAMFDEN+K+  PE+E
Sbjct: 261 GWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKS--PEIE 320

Query: 302 KHWGLFSPSKMPKYEVNFN 318
           K++GLF P+K PK+ + F+
Sbjct: 321 KNFGLFFPNKQPKFPITFS 335

[Close]

BLAST of CmaCh10G008210 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 258.5 bits (659), Expect = 7.2e-69
Identity = 129/326 (39.57%), Postives = 211/326 (64.72%), Query Frame = 0


Query: 2   GAQIGVCYGQLGNDLPSPVEVIELYKQNNIQRMRLYAPDHPTFDALRGSNIELMLGIPNN 61
           G ++GVCYG+  +DLP+P +V++L +Q+NI+ +R+Y  +     A   ++IELM+G+PN+
Sbjct: 23  GGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNS 82

Query: 62  ELEDLANFQEKADSWVHDNLVS-FGDVKFKYIVVGNEIKTND---QAASFLVPAMQNIQN 121
           +L   +  Q   D+W+ ++++  +   K  YI VG E  T+D    A+SF+VPAMQN+  
Sbjct: 83  DLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAE-STDDPHINASSFVVPAMQNVLT 142

Query: 122 AISAVGLGGQIKVSTAFDTGILAESYPPSKGSFKTDYLPILNPTIRFLLDNNAPLLVNVY 181
           A+  VGL  +IKVST    GIL+ S+PPS G+F + Y   L P + FL +N +P ++++Y
Sbjct: 143 ALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLY 202

Query: 182 PYFSYVANTADISMDYALFRGTSPSVEDGQ-FVYHNLFDAILDAVYSALEKAGSDGLEIA 241
           PY++Y  +  ++S+DY LF  +S  ++     +Y N+FDA +DA+Y AL       ++I 
Sbjct: 203 PYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIM 262

Query: 242 VSETGWPTEGG----EAATVENANTYNNNLIQHV--KEGTPKRSGKPVETYVFAMFDENE 301
           V+ETGWPT+G      AA+ +NA TYN+N+I+HV   +GTP + G+ +  Y+F++F+EN 
Sbjct: 263 VTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENR 322

Query: 302 KTTPPEVEKHWGLFSPSKMPKYEVNF 317
           K    + E++WGLF P +   Y+++F
Sbjct: 323 K-AGLDSERNWGLFYPDQTSVYQLDF 346

The following BLAST results are available for this feature:

BLAST of CmaCh10G008210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52408	8.0e-113	60.76	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS...	[more]
Q9M2M0	1.3e-110	59.49	Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q03773	1.3e-110	61.95	Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1	[more]
P36401	1.7e-102	57.50	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4...	[more]
P33157	1.9e-98	57.78	Glucan endo-1,3-beta-glucosidase, acidic isoform OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of CmaCh10G008210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JIH6	1.6e-180	100.00	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484756 PE=...	[more]
A0A6J1HCY3	2.0e-175	97.78	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461697 P...	[more]
A0A6J1CBL7	7.2e-141	78.41	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010152 ...	[more]
A0A0A0LYQ8	2.6e-135	76.85	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3...	[more]
A0A1S3C9Z3	1.4e-133	75.32	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103498316 PE=3 SV...	[more]

BLAST of CmaCh10G008210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022987103.1	3.3e-180	100.00	probable glucan endo-1,3-beta-glucosidase BG1 [Cucurbita maxima]	[more]
XP_022961079.1	4.1e-175	97.78	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata]	[more]
KAG6590085.1	3.5e-174	96.21	Glucan endo-1,3-beta-glucosidase, basic isoform, partial [Cucurbita argyrosperma...	[more]
XP_023516024.1	1.4e-154	96.13	glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita pepo subsp. pepo...	[more]
XP_022139185.1	1.5e-140	78.41	glucan endo-1,3-beta-glucosidase-like [Momordica charantia]	[more]

BLAST of CmaCh10G008210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57270.1	9.0e-112	59.49	beta-1,3-glucanase 1	[more]
AT3G57260.1	1.4e-99	57.78	beta-1,3-glucanase 2	[more]
AT3G57240.1	5.7e-98	57.46	beta-1,3-glucanase 3	[more]
AT4G16260.1	3.2e-85	48.28	Glycosyl hydrolase superfamily protein	[more]
AT5G56590.1	7.2e-69	39.57	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 5..316 e-value: 8.3E-121 score: 403.4
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 233..246
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 3..317 e-value: 3.3E-132 score: 442.3
None	No IPR available	PANTHER	PTHR32227:SF138	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATED	coord: 4..314
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 4..314
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 5..315

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh10G008210.1	CmaCh10G008210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds