CmaCh10G006850 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G006850
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionInositol oxygenase
LocationCma_Chr10: 3109537 .. 3111493 (-)
RNA-Seq ExpressionCmaCh10G006850
SyntenyCmaCh10G006850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATAAAGAAACCCCCTCCACCAATTCTCCTCACTCCGAATCTCATTCATATTCCGTTCTTTTGGATTCTTTGATTCTTGTGAAGAACATGACGATTCTCATCGAGCAGCCTCGCTTTGGTAACATCTTCTTGTTCTTCACCATTTACTTTTCATAAACATTTGAATTTGCAGCGAATTCTTGAGGGTTCGAAATGTTCTTGTATTTGCAGAAGTGGAAGAGAAGAAGCTTGGCATTATAGAGCCGAATGAATTGGTGTTGGACGGCGGATTTGTGGTACCAAATGGAACGGAAGCGGCGGAGGAGGGATTTGTAGCACTGGAAATGAATGCCTTCGGGCATTCTTTCAGAGATTACGAAGCGGAAAGCGAGAGGCAAAAGGGAGTGGAGGAATTTTATAAACAGAATCATATTCATCAAACGTACGATTACGTGAAGAAGATGAGAGAAACGTACAAGAAAATGGATCGAGTGGAAATGAGTATCTGGGAATGCTGTGAGCTTCTGAACGATGTGGTTGATGATAGCGATCCTGATTTGGATGAACCCCAAATTCAGCATTTGTTGCAGACTGCTGAAGCCATTCGAAAGGATTATCCTGATGAAGATTGGCTGCATTTGACTGCGCTCATCCATGGTATTATACCATCAAATTTCCTTCGACTCTGTTTTCTCTCTATTTCACTTTGTAACGATATGCTCTGTTTTTAGATCTTGGAAAAGTGCTTCTTCTTCCCAGCTTTGGTGGGCTTCCTCAATGGGCTGTTGTGGGTAAGAACAAACAACAAAACCCCATAATTTTTCTGTCATTTTCTTTCCCAGCAACACTCAAATCCATCTTCCCCTGTTTTGTTCTTGCAGGTGATACACACCCTCTTGGCTGCGCTTTTGATAGCTCCATTGTTCATCACAAGGTAGTTCATAATTCTGTAAATTCGAGATTTCTTTCCTTCAAATTACGAACAATAAAACTAATTTATTTGGGTTTTAATTTCAGTATTTCAAGGAGAATCCTGATTCGGAGAATCCAGCTTATAACACCAAAAATGGGATTTACACCGAAGGCTGCGGACTTGATAATGTTATGATATCATGGGGCCATGATGATTATATGTATTTGGTACCAAATCCATTCTTTGTGCCCCTTTTCTGGTAAAGCTTTGATTTTCGAAGCTCTGTTTGTTTAACAATGATTTGTGATGTAAATTTTGCAGGTGGCTAAAGAAAATGGCTCCACTTTGCCTTCGGCTGGGTTGTTCATAATCAGATACCACTCCTTTTATCGTAAGCAAATAAAATTCAAAATTAAAACAAAAAAGAATTGAAACTAAAAAGAATTCATTTTCCTGCAGCCTTACATAAAGCTGGAGCTTACAAACACTTAATGAACGAGGAGGATGCCCAGAATTTGAAGTGGCTCCATATATTCAAGTAATTATTTTTAAAAGATAATTACTGTTTTAATCCAAGATAATTGGAGTGATTATGAACTAATTATATATTTATGGAACTTCAGCAAGTATGATCTCTACAGTAAGAGCAAAGAACTTGTTGACGTGGAAAAAGTTAAACCATATTATGTTTCCCTAATTAACAAGGTAAAATCCTGTGCCTTTTTTATTTTATTTTTCAAATATTTTTGGGGACTAAATGGTGTAAATTATTGGGATTTGCAGTACTTCCCGGAAAACCTCCGATGGTGACCAGATATAATTAACGATGCCTACCGCCGGCCGGCGACGGATGACACATTTGAATGAATAAGCTTCATTTTTCTTGTATTATTATCTCATTTCTCCACATTAAATGAGTTCTTTTTTTCTTTTTCTTTCTTTTTTTCGCTAAATGTAACGGAAGAAATTGCGTCCCACCAAAGCAAATTAAAAATTTAATTGTTTTTCTCCTATGTGTTTGACCGTAACAATAATTAATTATTATATAAAAAAATTAAGAACAC

mRNA sequence

GTATAAAGAAACCCCCTCCACCAATTCTCCTCACTCCGAATCTCATTCATATTCCGTTCTTTTGGATTCTTTGATTCTTGTGAAGAACATGACGATTCTCATCGAGCAGCCTCGCTTTGAAGTGGAAGAGAAGAAGCTTGGCATTATAGAGCCGAATGAATTGGTGTTGGACGGCGGATTTGTGGTACCAAATGGAACGGAAGCGGCGGAGGAGGGATTTGTAGCACTGGAAATGAATGCCTTCGGGCATTCTTTCAGAGATTACGAAGCGGAAAGCGAGAGGCAAAAGGGAGTGGAGGAATTTTATAAACAGAATCATATTCATCAAACGTACGATTACGTGAAGAAGATGAGAGAAACGTACAAGAAAATGGATCGAGTGGAAATGAGTATCTGGGAATGCTGTGAGCTTCTGAACGATGTGGTTGATGATAGCGATCCTGATTTGGATGAACCCCAAATTCAGCATTTGTTGCAGACTGCTGAAGCCATTCGAAAGGATTATCCTGATGAAGATTGGCTGCATTTGACTGCGCTCATCCATGATCTTGGAAAAGTGCTTCTTCTTCCCAGCTTTGGTGGGCTTCCTCAATGGGCTGTTGTGGGTGATACACACCCTCTTGGCTGCGCTTTTGATAGCTCCATTGTTCATCACAAGTATTTCAAGGAGAATCCTGATTCGGAGAATCCAGCTTATAACACCAAAAATGGGATTTACACCGAAGGCTGCGGACTTGATAATGTTATGATATCATGGGGCCATGATGATTATATGTATTTGGTGGCTAAAGAAAATGGCTCCACTTTGCCTTCGGCTGGGTTGTTCATAATCAGATACCACTCCTTTTATCCCTTACATAAAGCTGGAGCTTACAAACACTTAATGAACGAGGAGGATGCCCAGAATTTGAAGTGGCTCCATATATTCAACAAGTATGATCTCTACAGTAAGAGCAAAGAACTTGTTGACGTGGAAAAAGTTAAACCATATTATGTTTCCCTAATTAACAAGTACTTCCCGGAAAACCTCCGATGGTGACCAGATATAATTAACGATGCCTACCGCCGGCCGGCGACGGATGACACATTTGAATGAATAAGCTTCATTTTTCTTGTATTATTATCTCATTTCTCCACATTAAATGAGTTCTTTTTTTCTTTTTCTTTCTTTTTTTCGCTAAATGTAACGGAAGAAATTGCGTCCCACCAAAGCAAATTAAAAATTTAATTGTTTTTCTCCTATGTGTTTGACCGTAACAATAATTAATTATTATATAAAAAAATTAAGAACAC

Coding sequence (CDS)

ATGACGATTCTCATCGAGCAGCCTCGCTTTGAAGTGGAAGAGAAGAAGCTTGGCATTATAGAGCCGAATGAATTGGTGTTGGACGGCGGATTTGTGGTACCAAATGGAACGGAAGCGGCGGAGGAGGGATTTGTAGCACTGGAAATGAATGCCTTCGGGCATTCTTTCAGAGATTACGAAGCGGAAAGCGAGAGGCAAAAGGGAGTGGAGGAATTTTATAAACAGAATCATATTCATCAAACGTACGATTACGTGAAGAAGATGAGAGAAACGTACAAGAAAATGGATCGAGTGGAAATGAGTATCTGGGAATGCTGTGAGCTTCTGAACGATGTGGTTGATGATAGCGATCCTGATTTGGATGAACCCCAAATTCAGCATTTGTTGCAGACTGCTGAAGCCATTCGAAAGGATTATCCTGATGAAGATTGGCTGCATTTGACTGCGCTCATCCATGATCTTGGAAAAGTGCTTCTTCTTCCCAGCTTTGGTGGGCTTCCTCAATGGGCTGTTGTGGGTGATACACACCCTCTTGGCTGCGCTTTTGATAGCTCCATTGTTCATCACAAGTATTTCAAGGAGAATCCTGATTCGGAGAATCCAGCTTATAACACCAAAAATGGGATTTACACCGAAGGCTGCGGACTTGATAATGTTATGATATCATGGGGCCATGATGATTATATGTATTTGGTGGCTAAAGAAAATGGCTCCACTTTGCCTTCGGCTGGGTTGTTCATAATCAGATACCACTCCTTTTATCCCTTACATAAAGCTGGAGCTTACAAACACTTAATGAACGAGGAGGATGCCCAGAATTTGAAGTGGCTCCATATATTCAACAAGTATGATCTCTACAGTAAGAGCAAAGAACTTGTTGACGTGGAAAAAGTTAAACCATATTATGTTTCCCTAATTAACAAGTACTTCCCGGAAAACCTCCGATGGTGA

Protein sequence

MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVKPYYVSLINKYFPENLRW
Homology
BLAST of CmaCh10G006850 vs. ExPASy Swiss-Prot
Match: O82200 (Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2)

HSP 1 Score: 517.7 bits (1332), Expect = 9.3e-146
Identity = 245/319 (76.80%), Postives = 275/319 (86.21%), Query Frame = 0

Query: 1   MTILIEQ--PRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRD 60
           MTIL+E   P   V+EKK+     NELVLDGGFVVP   E   + F A +MN  GHSFRD
Sbjct: 1   MTILVEHFVPDSRVDEKKVIEERDNELVLDGGFVVPKSKET--DAFDAPDMNFLGHSFRD 60

Query: 61  YE-AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSD 120
           YE  ESERQ+GVEEFY+  HIHQTYD+VKKMR+ Y K++++EMSIWECCELLN+VVD+SD
Sbjct: 61  YENGESERQQGVEEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVVDESD 120

Query: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180
           PDLDEPQIQHLLQTAEAIR+DYPDEDWLHLTALIHDLGKVLLLP FGGLPQWAVVGDT P
Sbjct: 121 PDLDEPQIQHLLQTAEAIRRDYPDEDWLHLTALIHDLGKVLLLPEFGGLPQWAVVGDTFP 180

Query: 181 LGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENG 240
           +GC FDS+ +HHKYFK N D  NP YNTKNG+YTEGCGLDNV++SWGHDDYMYLVAK+NG
Sbjct: 181 VGCTFDSANIHHKYFKGNHDINNPKYNTKNGVYTEGCGLDNVLMSWGHDDYMYLVAKKNG 240

Query: 241 STLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEK 300
           +TLP AGLFIIRYHSFYPLHKAGAY HLMN+ED  +LKWLH+FNKYDLYSKSK LVDVE+
Sbjct: 241 TTLPHAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLVDVEQ 300

Query: 301 VKPYYVSLINKYFPENLRW 317
           VKPYY+SLINKYFP  L+W
Sbjct: 301 VKPYYISLINKYFPAKLKW 317

BLAST of CmaCh10G006850 vs. ExPASy Swiss-Prot
Match: Q8H1S0 (Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 6.2e-142
Identity = 241/317 (76.03%), Postives = 266/317 (83.91%), Query Frame = 0

Query: 1   MTILIEQPRF-EVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDY 60
           MTI +E+P F EV   +       EL LDGGF +P      +E F+A EMNAFG  FRDY
Sbjct: 1   MTISVEKPIFEEVSAFEKSGDNIGELKLDGGFSMPKMDTNDDEAFLAPEMNAFGRQFRDY 60

Query: 61  EAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPD 120
           + ESERQKGVEEFY+  HI+QT D+VKKMR  Y K+D++ MSIWECCELLN+VVD+SDPD
Sbjct: 61  DVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDPD 120

Query: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLG 180
           LDEPQIQHLLQ+AEAIRKDYP+EDWLHLTALIHDLGKV+ LP FGGLPQWAVVGDT P+G
Sbjct: 121 LDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVGDTFPVG 180

Query: 181 CAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGST 240
           CAFD S VHHKYF ENPD  N  YNTKNGIY+EGCGL+NVM+SWGHDDYMYLVAKENGST
Sbjct: 181 CAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENGST 240

Query: 241 LPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVK 300
           LPSAG FIIRYHSFYPLH AG Y HLMNEED +NLKWLH+FNKYDLYSKSK  VDVEKVK
Sbjct: 241 LPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEKVK 300

Query: 301 PYYVSLINKYFPENLRW 317
           PYY+SLI KYFPENLRW
Sbjct: 301 PYYMSLIKKYFPENLRW 317

BLAST of CmaCh10G006850 vs. ExPASy Swiss-Prot
Match: Q8L799 (Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 1.2e-132
Identity = 229/324 (70.68%), Postives = 263/324 (81.17%), Query Frame = 0

Query: 1   MTILIEQ------PRFEVEEKKL--GIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAF 60
           MTILI++         E+ EK    G  E  ELVLD GF  P+              N+F
Sbjct: 1   MTILIDRHSDQNDAGDEIVEKNQGNGKEEETELVLDAGFEAPH-------------TNSF 60

Query: 61  GHSFRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDV 120
           G +FRDY+AESER++GVEEFY+ NHI QT D+V+KMRE Y+K++R EMSIWECCELLN+ 
Sbjct: 61  GRTFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNEF 120

Query: 121 VDDSDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVV 180
           +D+SDPDLDEPQI+HLLQTAEAIRKDYPDEDWLHLT LIHDLGKVLL  SFG LPQWAVV
Sbjct: 121 IDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLHSSFGELPQWAVV 180

Query: 181 GDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLV 240
           GDT P+GCAFD SIVHHKYFKENPD +NP+YN+K GIYTEGCGLDNV++SWGHDDYMYLV
Sbjct: 181 GDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMYLV 240

Query: 241 AKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL 300
           AKEN +TLPSAGLFIIRYHSFY LHK+ AYKHLMN ED +N+KWL +FNKYDLYSKSK  
Sbjct: 241 AKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSKVR 300

Query: 301 VDVEKVKPYYVSLINKYFPENLRW 317
           V+VE+VKPYY+SL NKYFP  L+W
Sbjct: 301 VNVEEVKPYYLSLTNKYFPSKLKW 311

BLAST of CmaCh10G006850 vs. ExPASy Swiss-Prot
Match: Q9FJU4 (Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 4.5e-132
Identity = 228/317 (71.92%), Postives = 257/317 (81.07%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDY- 60
           M I +E P F  E+      +  EL LD   +  +   + +E F+A EMNAFG  FRDY 
Sbjct: 1   MNISVENPVFVHEDSTTQ--KTGELRLDSD-IPMSKISSDDEVFLAPEMNAFGRQFRDYT 60

Query: 61  EAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPD 120
           +  SERQK VE FY   H +QT D+V+KMR  Y K+D++ M+IWECCEL  +VVD+SDPD
Sbjct: 61  DTNSERQKSVEHFYATQHTNQTLDFVQKMRSEYGKLDKMVMNIWECCELSKEVVDESDPD 120

Query: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLG 180
           LDEPQIQHLLQ+AEAIRKDYP+EDWLHLTALIHDLGKVL LP FGGLPQWAVVGDT P+G
Sbjct: 121 LDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVLTLPQFGGLPQWAVVGDTFPVG 180

Query: 181 CAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGST 240
           CAFD S VHHKYF ENPD  NP YNTK GIY+EGCGL+NV++SWGHDDYMYLVAKENGST
Sbjct: 181 CAFDESNVHHKYFMENPDFNNPKYNTKAGIYSEGCGLENVLMSWGHDDYMYLVAKENGST 240

Query: 241 LPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVK 300
           LPS GLFIIRYHSFYPLHKAGAY HLMNEED +NLKWLH+FNKYDLYSKSK  V+VEKVK
Sbjct: 241 LPSPGLFIIRYHSFYPLHKAGAYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVNVEKVK 300

Query: 301 PYYVSLINKYFPENLRW 317
           PYY+SLI KYFPENLRW
Sbjct: 301 PYYMSLIKKYFPENLRW 314

BLAST of CmaCh10G006850 vs. ExPASy Swiss-Prot
Match: Q5Z8T3 (Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0561000 PE=2 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 2.9e-131
Identity = 226/321 (70.40%), Postives = 260/321 (81.00%), Query Frame = 0

Query: 1   MTILIEQPRFE-VEEKKL----GIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHS 60
           MTI IEQP  + + ++K+    G     ELVLDGGFVVP             + NAFG++
Sbjct: 1   MTITIEQPHLDAIADRKVAGGGGGDNAAELVLDGGFVVP-------------DSNAFGNA 60

Query: 61  FRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDD 120
           FR+YEAESER++ VEEFY+ NHI+QTYD+V++MRE Y ++D+ EM IWEC ELLN+ +DD
Sbjct: 61  FRNYEAESERKETVEEFYRVNHINQTYDFVRRMREEYGRVDKTEMGIWECIELLNEFIDD 120

Query: 121 SDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDT 180
           SDPDLD PQI+HLLQTAEAIRKD+PDEDWLHLT LIHDLGKVLL PSFG LPQW+VVGDT
Sbjct: 121 SDPDLDMPQIEHLLQTAEAIRKDFPDEDWLHLTGLIHDLGKVLLHPSFGELPQWSVVGDT 180

Query: 181 HPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKE 240
            P+GCAFD   VH KYFKENPD  NP  NTK G Y+EGCGLDNV++SWGHDDYMYLVAKE
Sbjct: 181 FPVGCAFDECNVHFKYFKENPDYLNPKLNTKFGAYSEGCGLDNVLMSWGHDDYMYLVAKE 240

Query: 241 NGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDV 300
           N +TLPSAGLFIIRYHSFYPLHK GAY HLMN+ED +NLKWL +FNKYDLYSKS E +DV
Sbjct: 241 NKTTLPSAGLFIIRYHSFYPLHKHGAYMHLMNDEDKENLKWLRVFNKYDLYSKSNERIDV 300

Query: 301 EKVKPYYVSLINKYFPENLRW 317
           EKVKPYY+SLI KYFP  LRW
Sbjct: 301 EKVKPYYMSLIEKYFPAKLRW 308

BLAST of CmaCh10G006850 vs. ExPASy TrEMBL
Match: A0A6J1JI24 (Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485296 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 3.9e-187
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDYE 60
           MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDYE
Sbjct: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDYE 60

Query: 61  AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL 120
           AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL
Sbjct: 61  AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL 120

Query: 121 DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC 180
           DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC
Sbjct: 121 DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC 180

Query: 181 AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL 240
           AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL
Sbjct: 181 AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL 240

Query: 241 PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVKP 300
           PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVKP
Sbjct: 241 PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVKP 300

Query: 301 YYVSLINKYFPENLRW 317
           YYVSLINKYFPENLRW
Sbjct: 301 YYVSLINKYFPENLRW 316

BLAST of CmaCh10G006850 vs. ExPASy TrEMBL
Match: A0A6J1HBL6 (Inositol oxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461362 PE=3 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.3e-182
Identity = 309/316 (97.78%), Postives = 311/316 (98.42%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDYE 60
           MTILIEQPRFEVEEKK+GI E NELVLDGGFVVPN T AAEEGFVALEMNAFGHSFRDYE
Sbjct: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAEEGFVALEMNAFGHSFRDYE 60

Query: 61  AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL 120
           AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL
Sbjct: 61  AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL 120

Query: 121 DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC 180
           DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC
Sbjct: 121 DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC 180

Query: 181 AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL 240
           AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL
Sbjct: 181 AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL 240

Query: 241 PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVKP 300
           PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL+DVEKVKP
Sbjct: 241 PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKVKP 300

Query: 301 YYVSLINKYFPENLRW 317
           YYVSLINKYFPE LRW
Sbjct: 301 YYVSLINKYFPEKLRW 316

BLAST of CmaCh10G006850 vs. ExPASy TrEMBL
Match: A0A1S3BQR6 (Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103492281 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.5e-167
Identity = 285/317 (89.91%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 1   MTILIEQPRFEVE-EKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDY 60
           MTILIEQP FEVE EKK+G+ E  ELVLDGGFVVPN      EGF A EMNAFGHSFRDY
Sbjct: 1   MTILIEQPHFEVEDEKKVGMTELKELVLDGGFVVPNA--RTTEGFAAPEMNAFGHSFRDY 60

Query: 61  EAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPD 120
           EAESERQKGVEEFY++NHIHQTY++V KMRE YKKMDRVEMSIWECCELLNDVVDDSDPD
Sbjct: 61  EAESERQKGVEEFYRKNHIHQTYEFVTKMREEYKKMDRVEMSIWECCELLNDVVDDSDPD 120

Query: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLG 180
           LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP+G
Sbjct: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPVG 180

Query: 181 CAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGST 240
           CAFD SIVHHKYFKENPD ENP Y+TKNGIY+EGCGL+NVMISWGHDDYMYLVAKENGST
Sbjct: 181 CAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLNNVMISWGHDDYMYLVAKENGST 240

Query: 241 LPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVK 300
           LPSAGLFIIRYHSFYPLH+AGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL+DVEKVK
Sbjct: 241 LPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKVK 300

Query: 301 PYYVSLINKYFPENLRW 317
           PYY+SLINKYFPE LRW
Sbjct: 301 PYYISLINKYFPEKLRW 315

BLAST of CmaCh10G006850 vs. ExPASy TrEMBL
Match: A0A0A0LZA8 (Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G575130 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.6e-167
Identity = 286/317 (90.22%), Postives = 297/317 (93.69%), Query Frame = 0

Query: 1   MTILIEQPRFEVE-EKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDY 60
           MTILIEQP FE E EKK+GI E  ELVLDGGFVVPN   A  EGF A EMNAFGHSFRDY
Sbjct: 1   MTILIEQPHFEGEDEKKVGITELKELVLDGGFVVPN---AKTEGFAAPEMNAFGHSFRDY 60

Query: 61  EAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPD 120
           EAESERQKGVEEFY+QNHIHQTY++V+KMRE YKKMDR EMSIWECCELLNDVVDDSDPD
Sbjct: 61  EAESERQKGVEEFYRQNHIHQTYEFVEKMREEYKKMDREEMSIWECCELLNDVVDDSDPD 120

Query: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLG 180
           LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP+G
Sbjct: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPVG 180

Query: 181 CAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGST 240
           CAFD SIVHHKYFKENPD ENP Y+TKNGIY+EGCGLDNV ISWGHDDYMYLVAKENGST
Sbjct: 181 CAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLDNVKISWGHDDYMYLVAKENGST 240

Query: 241 LPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVK 300
           LPSAGLFIIRYHSFYPLH+AGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL+DVEKVK
Sbjct: 241 LPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKVK 300

Query: 301 PYYVSLINKYFPENLRW 317
           PYY+SLINKYFPE LRW
Sbjct: 301 PYYISLINKYFPEKLRW 314

BLAST of CmaCh10G006850 vs. ExPASy TrEMBL
Match: A0A6J1CSQ8 (Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111014416 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 1.9e-162
Identity = 279/320 (87.19%), Postives = 292/320 (91.25%), Query Frame = 0

Query: 1   MTILIEQPR-FEVEEKKLGIIEPNELVLDGGFVVPN---GTEAAEEGFVALEMNAFGHSF 60
           MTILI+ P   EVEEKK  I E  EL LDGGFV+P+      AA EGFVA E+N+FG SF
Sbjct: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60

Query: 61  RDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKEN 240
           PLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMISWGHDDYMYLVAKEN
Sbjct: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVE 300
           GSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE +DVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300

Query: 301 KVKPYYVSLINKYFPENLRW 317
           KVKPYY+SLINKYFPE LRW
Sbjct: 301 KVKPYYMSLINKYFPEKLRW 320

BLAST of CmaCh10G006850 vs. NCBI nr
Match: XP_022987889.1 (inositol oxygenase 2-like [Cucurbita maxima] >XP_022987890.1 inositol oxygenase 2-like [Cucurbita maxima])

HSP 1 Score: 663.7 bits (1711), Expect = 8.0e-187
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDYE 60
           MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDYE
Sbjct: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDYE 60

Query: 61  AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL 120
           AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL
Sbjct: 61  AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL 120

Query: 121 DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC 180
           DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC
Sbjct: 121 DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC 180

Query: 181 AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL 240
           AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL
Sbjct: 181 AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL 240

Query: 241 PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVKP 300
           PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVKP
Sbjct: 241 PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVKP 300

Query: 301 YYVSLINKYFPENLRW 317
           YYVSLINKYFPENLRW
Sbjct: 301 YYVSLINKYFPENLRW 316

BLAST of CmaCh10G006850 vs. NCBI nr
Match: XP_022960634.1 (inositol oxygenase 2-like [Cucurbita moschata] >KAG6589967.1 Inositol oxygenase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 648.7 bits (1672), Expect = 2.7e-182
Identity = 309/316 (97.78%), Postives = 311/316 (98.42%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDYE 60
           MTILIEQPRFEVEEKK+GI E NELVLDGGFVVPN T AAEEGFVALEMNAFGHSFRDYE
Sbjct: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAEEGFVALEMNAFGHSFRDYE 60

Query: 61  AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL 120
           AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL
Sbjct: 61  AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDL 120

Query: 121 DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC 180
           DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC
Sbjct: 121 DEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGC 180

Query: 181 AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL 240
           AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL
Sbjct: 181 AFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTL 240

Query: 241 PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVKP 300
           PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL+DVEKVKP
Sbjct: 241 PSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKVKP 300

Query: 301 YYVSLINKYFPENLRW 317
           YYVSLINKYFPE LRW
Sbjct: 301 YYVSLINKYFPEKLRW 316

BLAST of CmaCh10G006850 vs. NCBI nr
Match: XP_023515618.1 (inositol oxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG7023630.1 Inositol oxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 643.7 bits (1659), Expect = 8.6e-181
Identity = 309/318 (97.17%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGT--EAAEEGFVALEMNAFGHSFRD 60
           MTILIEQPRFEVEEKK+GI E NELVLDGGFVVPN T   AAEEGFVALEMNAFGHSFRD
Sbjct: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRD 60

Query: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120
           YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP
Sbjct: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120

Query: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180
           DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL
Sbjct: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180

Query: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240
           GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS
Sbjct: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240

Query: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKV 300
           TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL+DVEKV
Sbjct: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV 300

Query: 301 KPYYVSLINKYFPENLRW 317
           KPYYVSLINKYFPE LRW
Sbjct: 301 KPYYVSLINKYFPEKLRW 318

BLAST of CmaCh10G006850 vs. NCBI nr
Match: XP_008450805.1 (PREDICTED: inositol oxygenase 2-like [Cucumis melo])

HSP 1 Score: 598.6 bits (1542), Expect = 3.2e-167
Identity = 285/317 (89.91%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 1   MTILIEQPRFEVE-EKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDY 60
           MTILIEQP FEVE EKK+G+ E  ELVLDGGFVVPN      EGF A EMNAFGHSFRDY
Sbjct: 1   MTILIEQPHFEVEDEKKVGMTELKELVLDGGFVVPNA--RTTEGFAAPEMNAFGHSFRDY 60

Query: 61  EAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPD 120
           EAESERQKGVEEFY++NHIHQTY++V KMRE YKKMDRVEMSIWECCELLNDVVDDSDPD
Sbjct: 61  EAESERQKGVEEFYRKNHIHQTYEFVTKMREEYKKMDRVEMSIWECCELLNDVVDDSDPD 120

Query: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLG 180
           LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP+G
Sbjct: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPVG 180

Query: 181 CAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGST 240
           CAFD SIVHHKYFKENPD ENP Y+TKNGIY+EGCGL+NVMISWGHDDYMYLVAKENGST
Sbjct: 181 CAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLNNVMISWGHDDYMYLVAKENGST 240

Query: 241 LPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVK 300
           LPSAGLFIIRYHSFYPLH+AGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL+DVEKVK
Sbjct: 241 LPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKVK 300

Query: 301 PYYVSLINKYFPENLRW 317
           PYY+SLINKYFPE LRW
Sbjct: 301 PYYISLINKYFPEKLRW 315

BLAST of CmaCh10G006850 vs. NCBI nr
Match: XP_011659961.1 (inositol oxygenase 2 [Cucumis sativus] >KGN66197.1 hypothetical protein Csa_007517 [Cucumis sativus])

HSP 1 Score: 597.8 bits (1540), Expect = 5.4e-167
Identity = 286/317 (90.22%), Postives = 297/317 (93.69%), Query Frame = 0

Query: 1   MTILIEQPRFEVE-EKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDY 60
           MTILIEQP FE E EKK+GI E  ELVLDGGFVVPN   A  EGF A EMNAFGHSFRDY
Sbjct: 1   MTILIEQPHFEGEDEKKVGITELKELVLDGGFVVPN---AKTEGFAAPEMNAFGHSFRDY 60

Query: 61  EAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPD 120
           EAESERQKGVEEFY+QNHIHQTY++V+KMRE YKKMDR EMSIWECCELLNDVVDDSDPD
Sbjct: 61  EAESERQKGVEEFYRQNHIHQTYEFVEKMREEYKKMDREEMSIWECCELLNDVVDDSDPD 120

Query: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLG 180
           LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP+G
Sbjct: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPVG 180

Query: 181 CAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGST 240
           CAFD SIVHHKYFKENPD ENP Y+TKNGIY+EGCGLDNV ISWGHDDYMYLVAKENGST
Sbjct: 181 CAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLDNVKISWGHDDYMYLVAKENGST 240

Query: 241 LPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVK 300
           LPSAGLFIIRYHSFYPLH+AGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL+DVEKVK
Sbjct: 241 LPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKVK 300

Query: 301 PYYVSLINKYFPENLRW 317
           PYY+SLINKYFPE LRW
Sbjct: 301 PYYISLINKYFPEKLRW 314

BLAST of CmaCh10G006850 vs. TAIR 10
Match: AT2G19800.1 (myo-inositol oxygenase 2 )

HSP 1 Score: 517.7 bits (1332), Expect = 6.6e-147
Identity = 245/319 (76.80%), Postives = 275/319 (86.21%), Query Frame = 0

Query: 1   MTILIEQ--PRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRD 60
           MTIL+E   P   V+EKK+     NELVLDGGFVVP   E   + F A +MN  GHSFRD
Sbjct: 1   MTILVEHFVPDSRVDEKKVIEERDNELVLDGGFVVPKSKET--DAFDAPDMNFLGHSFRD 60

Query: 61  YE-AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSD 120
           YE  ESERQ+GVEEFY+  HIHQTYD+VKKMR+ Y K++++EMSIWECCELLN+VVD+SD
Sbjct: 61  YENGESERQQGVEEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVVDESD 120

Query: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180
           PDLDEPQIQHLLQTAEAIR+DYPDEDWLHLTALIHDLGKVLLLP FGGLPQWAVVGDT P
Sbjct: 121 PDLDEPQIQHLLQTAEAIRRDYPDEDWLHLTALIHDLGKVLLLPEFGGLPQWAVVGDTFP 180

Query: 181 LGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENG 240
           +GC FDS+ +HHKYFK N D  NP YNTKNG+YTEGCGLDNV++SWGHDDYMYLVAK+NG
Sbjct: 181 VGCTFDSANIHHKYFKGNHDINNPKYNTKNGVYTEGCGLDNVLMSWGHDDYMYLVAKKNG 240

Query: 241 STLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEK 300
           +TLP AGLFIIRYHSFYPLHKAGAY HLMN+ED  +LKWLH+FNKYDLYSKSK LVDVE+
Sbjct: 241 TTLPHAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLVDVEQ 300

Query: 301 VKPYYVSLINKYFPENLRW 317
           VKPYY+SLINKYFP  L+W
Sbjct: 301 VKPYYISLINKYFPAKLKW 317

BLAST of CmaCh10G006850 vs. TAIR 10
Match: AT4G26260.2 (myo-inositol oxygenase 4 )

HSP 1 Score: 507.3 bits (1305), Expect = 8.9e-144
Identity = 242/318 (76.10%), Postives = 266/318 (83.65%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKLGIIEPN--ELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRD 60
           MTI +E+P FE E         N  EL LDGGF +P      +E F+A EMNAFG  FRD
Sbjct: 1   MTISVEKPIFEEEVSAFEKSGDNIGELKLDGGFSMPKMDTNDDEAFLAPEMNAFGRQFRD 60

Query: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120
           Y+ ESERQKGVEEFY+  HI+QT D+VKKMR  Y K+D++ MSIWECCELLN+VVD+SDP
Sbjct: 61  YDVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDP 120

Query: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180
           DLDEPQIQHLLQ+AEAIRKDYP+EDWLHLTALIHDLGKV+ LP FGGLPQWAVVGDT P+
Sbjct: 121 DLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVGDTFPV 180

Query: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240
           GCAFD S VHHKYF ENPD  N  YNTKNGIY+EGCGL+NVM+SWGHDDYMYLVAKENGS
Sbjct: 181 GCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENGS 240

Query: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKV 300
           TLPSAG FIIRYHSFYPLH AG Y HLMNEED +NLKWLH+FNKYDLYSKSK  VDVEKV
Sbjct: 241 TLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEKV 300

Query: 301 KPYYVSLINKYFPENLRW 317
           KPYY+SLI KYFPENLRW
Sbjct: 301 KPYYMSLIKKYFPENLRW 318

BLAST of CmaCh10G006850 vs. TAIR 10
Match: AT4G26260.1 (myo-inositol oxygenase 4 )

HSP 1 Score: 505.0 bits (1299), Expect = 4.4e-143
Identity = 241/317 (76.03%), Postives = 266/317 (83.91%), Query Frame = 0

Query: 1   MTILIEQPRF-EVEEKKLGIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGHSFRDY 60
           MTI +E+P F EV   +       EL LDGGF +P      +E F+A EMNAFG  FRDY
Sbjct: 1   MTISVEKPIFEEVSAFEKSGDNIGELKLDGGFSMPKMDTNDDEAFLAPEMNAFGRQFRDY 60

Query: 61  EAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPD 120
           + ESERQKGVEEFY+  HI+QT D+VKKMR  Y K+D++ MSIWECCELLN+VVD+SDPD
Sbjct: 61  DVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDESDPD 120

Query: 121 LDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLG 180
           LDEPQIQHLLQ+AEAIRKDYP+EDWLHLTALIHDLGKV+ LP FGGLPQWAVVGDT P+G
Sbjct: 121 LDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVGDTFPVG 180

Query: 181 CAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGST 240
           CAFD S VHHKYF ENPD  N  YNTKNGIY+EGCGL+NVM+SWGHDDYMYLVAKENGST
Sbjct: 181 CAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENGST 240

Query: 241 LPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKVK 300
           LPSAG FIIRYHSFYPLH AG Y HLMNEED +NLKWLH+FNKYDLYSKSK  VDVEKVK
Sbjct: 241 LPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEKVK 300

Query: 301 PYYVSLINKYFPENLRW 317
           PYY+SLI KYFPENLRW
Sbjct: 301 PYYMSLIKKYFPENLRW 317

BLAST of CmaCh10G006850 vs. TAIR 10
Match: AT1G14520.2 (myo-inositol oxygenase 1 )

HSP 1 Score: 476.1 bits (1224), Expect = 2.2e-134
Identity = 230/322 (71.43%), Postives = 263/322 (81.68%), Query Frame = 0

Query: 1   MTILI----EQPRFEVEEKKL--GIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAFGH 60
           MTILI    +Q   E+ EK    G  E  ELVLD GF  P+              N+FG 
Sbjct: 1   MTILIDRHSDQNGDEIVEKNQGNGKEEETELVLDAGFEAPH-------------TNSFGR 60

Query: 61  SFRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVD 120
           +FRDY+AESER++GVEEFY+ NHI QT D+V+KMRE Y+K++R EMSIWECCELLN+ +D
Sbjct: 61  TFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNEFID 120

Query: 121 DSDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGD 180
           +SDPDLDEPQI+HLLQTAEAIRKDYPDEDWLHLT LIHDLGKVLL  SFG LPQWAVVGD
Sbjct: 121 ESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLHSSFGELPQWAVVGD 180

Query: 181 THPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAK 240
           T P+GCAFD SIVHHKYFKENPD +NP+YN+K GIYTEGCGLDNV++SWGHDDYMYLVAK
Sbjct: 181 TFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMYLVAK 240

Query: 241 ENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVD 300
           EN +TLPSAGLFIIRYHSFY LHK+ AYKHLMN ED +N+KWL +FNKYDLYSKSK  V+
Sbjct: 241 ENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSKVRVN 300

Query: 301 VEKVKPYYVSLINKYFPENLRW 317
           VE+VKPYY+SL NKYFP  L+W
Sbjct: 301 VEEVKPYYLSLTNKYFPSKLKW 309

BLAST of CmaCh10G006850 vs. TAIR 10
Match: AT1G14520.1 (myo-inositol oxygenase 1 )

HSP 1 Score: 474.2 bits (1219), Expect = 8.4e-134
Identity = 229/324 (70.68%), Postives = 263/324 (81.17%), Query Frame = 0

Query: 1   MTILIEQ------PRFEVEEKKL--GIIEPNELVLDGGFVVPNGTEAAEEGFVALEMNAF 60
           MTILI++         E+ EK    G  E  ELVLD GF  P+              N+F
Sbjct: 1   MTILIDRHSDQNDAGDEIVEKNQGNGKEEETELVLDAGFEAPH-------------TNSF 60

Query: 61  GHSFRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDV 120
           G +FRDY+AESER++GVEEFY+ NHI QT D+V+KMRE Y+K++R EMSIWECCELLN+ 
Sbjct: 61  GRTFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNEF 120

Query: 121 VDDSDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVV 180
           +D+SDPDLDEPQI+HLLQTAEAIRKDYPDEDWLHLT LIHDLGKVLL  SFG LPQWAVV
Sbjct: 121 IDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLHSSFGELPQWAVV 180

Query: 181 GDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLV 240
           GDT P+GCAFD SIVHHKYFKENPD +NP+YN+K GIYTEGCGLDNV++SWGHDDYMYLV
Sbjct: 181 GDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMYLV 240

Query: 241 AKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL 300
           AKEN +TLPSAGLFIIRYHSFY LHK+ AYKHLMN ED +N+KWL +FNKYDLYSKSK  
Sbjct: 241 AKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSKVR 300

Query: 301 VDVEKVKPYYVSLINKYFPENLRW 317
           V+VE+VKPYY+SL NKYFP  L+W
Sbjct: 301 VNVEEVKPYYLSLTNKYFPSKLKW 311

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O822009.3e-14676.80Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2[more]
Q8H1S06.2e-14276.03Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1[more]
Q8L7991.2e-13270.68Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1[more]
Q9FJU44.5e-13271.92Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1[more]
Q5Z8T32.9e-13170.40Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g056... [more]
Match NameE-valueIdentityDescription
A0A6J1JI243.9e-187100.00Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485296 PE=3 SV=1[more]
A0A6J1HBL61.3e-18297.78Inositol oxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461362 PE=3 SV=1[more]
A0A1S3BQR61.5e-16789.91Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103492281 PE=3 SV=1[more]
A0A0A0LZA82.6e-16790.22Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G575130 PE=3 SV=1[more]
A0A6J1CSQ81.9e-16287.19Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111014416 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022987889.18.0e-187100.00inositol oxygenase 2-like [Cucurbita maxima] >XP_022987890.1 inositol oxygenase ... [more]
XP_022960634.12.7e-18297.78inositol oxygenase 2-like [Cucurbita moschata] >KAG6589967.1 Inositol oxygenase ... [more]
XP_023515618.18.6e-18197.17inositol oxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG7023630.1 Inositol ox... [more]
XP_008450805.13.2e-16789.91PREDICTED: inositol oxygenase 2-like [Cucumis melo][more]
XP_011659961.15.4e-16790.22inositol oxygenase 2 [Cucumis sativus] >KGN66197.1 hypothetical protein Csa_0075... [more]
Match NameE-valueIdentityDescription
AT2G19800.16.6e-14776.80myo-inositol oxygenase 2 [more]
AT4G26260.28.9e-14476.10myo-inositol oxygenase 4 [more]
AT4G26260.14.4e-14376.03myo-inositol oxygenase 4 [more]
AT1G14520.22.2e-13471.43myo-inositol oxygenase 1 [more]
AT1G14520.18.4e-13470.68myo-inositol oxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007828Inositol oxygenasePFAMPF05153MIOXcoord: 67..316
e-value: 4.7E-125
score: 416.1
IPR007828Inositol oxygenasePANTHERPTHR12588MYOINOSITOL OXYGENASEcoord: 1..316
NoneNo IPR availablePANTHERPTHR12588:SF14INOSITOL OXYGENASE 5coord: 1..316
NoneNo IPR availableSUPERFAMILY109604HD-domain/PDEase-likecoord: 60..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G006850.1CmaCh10G006850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019310 inositol catabolic process
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050113 inositol oxygenase activity
molecular_function GO:0005506 iron ion binding