CmaCh10G003420 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh10G003420
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	beta-amyrin 28-oxidase-like
Location	Cma_Chr10: 1593680 .. 1594096 (-)
RNA-Seq Expression	CmaCh10G003420
Synteny	CmaCh10G003420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAATCTGTGTTTCTCTCTCTTCATAATCTTAGCTTCGTTTCTCTCTCTTGCCCTGTTCATCCTGTTCTACCGCCACAGAGCTCAATTCGCCTACCCGAACATTCCACCGGGCAGCTTCTGGTATCCGATCATCGGAGAGACTCTTGAAATGGCTTCCTACGAGAAGAAAGGGCAGCCGGAGAATTTCATTTTTGGTCGGGCGGCCAAGTACAAGACGGATGTTTTCACTACTTCCGTCATTGGGAAACCGACGGCCGCCTTCTGCTTGGCGGCGGGGAATAAGCTTCTGTTCTCGAACGAGCAGAAACTGGTGACGCCGTGGTGGCCGCCGTCTGCTGATAAAGTTTTTCCGTCGACCGTTAAGTATAGTTCTAGTGAAGAAGCGAGGAGGATTAAGAGTTTTCTCCCTTAG

mRNA sequence

ATGGAGAATCTGTGTTTCTCTCTCTTCATAATCTTAGCTTCGTTTCTCTCTCTTGCCCTGTTCATCCTGTTCTACCGCCACAGAGCTCAATTCGCCTACCCGAACATTCCACCGGGCAGCTTCTGGTATCCGATCATCGGAGAGACTCTTGAAATGGCTTCCTACGAGAAGAAAGGGCAGCCGGAGAATTTCATTTTTGGTCGGGCGGCCAAGTACAAGACGGATGTTTTCACTACTTCCGTCATTGGGAAACCGACGGCCGCCTTCTGCTTGGCGGCGGGGAATAAGCTTCTGTTCTCGAACGAGCAGAAACTGGTGACGCCGTGGTGGCCGCCGTCTGCTGATAAAGTTTTTCCGTCGACCGTTAAGTATAGTTCTAGTGAAGAAGCGAGGAGGATTAAGAGTTTTCTCCCTTAG

Coding sequence (CDS)

ATGGAGAATCTGTGTTTCTCTCTCTTCATAATCTTAGCTTCGTTTCTCTCTCTTGCCCTGTTCATCCTGTTCTACCGCCACAGAGCTCAATTCGCCTACCCGAACATTCCACCGGGCAGCTTCTGGTATCCGATCATCGGAGAGACTCTTGAAATGGCTTCCTACGAGAAGAAAGGGCAGCCGGAGAATTTCATTTTTGGTCGGGCGGCCAAGTACAAGACGGATGTTTTCACTACTTCCGTCATTGGGAAACCGACGGCCGCCTTCTGCTTGGCGGCGGGGAATAAGCTTCTGTTCTCGAACGAGCAGAAACTGGTGACGCCGTGGTGGCCGCCGTCTGCTGATAAAGTTTTTCCGTCGACCGTTAAGTATAGTTCTAGTGAAGAAGCGAGGAGGATTAAGAGTTTTCTCCCTTAG

Protein sequence

MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQPENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPSTVKYSSSEEARRIKSFLP

Homology

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BLAST of CmaCh10G003420 vs. ExPASy Swiss-Prot
Match: F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 4.5e-36
Identity = 72/138 (52.17%), Postives = 95/138 (68.84%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           ME    SL +I    +S+ L +LFY+HR+ F  PN+PPG   +P++GE+LE  S   KG 
Sbjct: 1   MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PE FIF R +KY ++VF TS++G+P A F  AAGNK LFSNE KLV  WWP S DKVFPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
           + + SS EEA++++  LP
Sbjct: 121 STQTSSKEEAKKMRKLLP 138

[Close]

BLAST of CmaCh10G003420 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 1.3e-35
Identity = 71/138 (51.45%), Postives = 95/138 (68.84%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           ME    SL +I    +S+ L++L Y+HR+ F  PN+PPG   +P++GE+LE  S   KG 
Sbjct: 1   MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PE FIF R +KY ++VF TS++G+P A F  AAGNK LFSNE KLV  WWP S DKVFPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
           + + SS EEA++++  LP
Sbjct: 121 STQTSSKEEAKKMRKLLP 138

[Close]

BLAST of CmaCh10G003420 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 6.3e-30
Identity = 65/137 (47.45%), Postives = 90/137 (65.69%), Query Frame = 0


Query: 3   NLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQPE 62
           N   SL ++  +F+SL+LF +FY+ ++     N+PPG   YPIIGE+LE  S   KG PE
Sbjct: 4   NFYLSLLLLFVTFISLSLFFIFYKQKSPL---NLPPGKMGYPIIGESLEFLSTGWKGHPE 63

Query: 63  NFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPSTV 122
            FIF R  KY +++F TS++G+ T   C AA NK LFSNE KLVT WWP S +K+FP+T 
Sbjct: 64  KFIFDRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTS 123

Query: 123 KYSS-SEEARRIKSFLP 139
             S+  EE+ +++  LP
Sbjct: 124 LDSNLKEESIKMRKLLP 137

[Close]

BLAST of CmaCh10G003420 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.1e-29
Identity = 67/133 (50.38%), Postives = 90/133 (67.67%), Query Frame = 0


Query: 7   SLFIILASFLSLALFILFYRHRA-QFAYPNIPPGSFWYPIIGETLEMASYEKKGQPENFI 66
           SLF++   F+SL+ + LFY+ ++   +   +PPG   +P+IGE+ E  S   KG PE FI
Sbjct: 10  SLFVL---FVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKGYPEKFI 69

Query: 67  FGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPSTVKYS 126
           F R AKY ++VF TS+ G+P AAFC AA NK LFSNE KLV  WWP S +KVFPS+ + S
Sbjct: 70  FDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFPSSTQTS 129

Query: 127 SSEEARRIKSFLP 139
           S EEA +++  LP
Sbjct: 130 SKEEAIKMRKLLP 139

[Close]

BLAST of CmaCh10G003420 vs. ExPASy Swiss-Prot
Match: I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.4e-28
Identity = 67/140 (47.86%), Postives = 87/140 (62.14%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHR--AQFAYPNIPPGSFWYPIIGETLEMASYEKK 60
           ME     L  +   F+SL+   LFY+ +  +   +P +PPG   +PIIGE+ E  S   K
Sbjct: 1   MELFYVPLLSLFVLFISLSFHFLFYKSKPSSSGGFP-LPPGKTGWPIIGESYEFLSTGWK 60

Query: 61  GQPENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVF 120
           G PE FIF R  KY ++VF TS+ G+P A FC AA NK LFSNE KLV  WWP S +KVF
Sbjct: 61  GYPEKFIFDRMTKYSSNVFKTSIFGEPAAVFCGAACNKFLFSNENKLVQAWWPDSVNKVF 120

Query: 121 PSTVKYSSSEEARRIKSFLP 139
           PS+ + SS EEA +++  LP
Sbjct: 121 PSSTQTSSKEEAIKMRKMLP 139

[Close]

BLAST of CmaCh10G003420 vs. ExPASy TrEMBL
Match: A0A6J1C307 (beta-amyrin 28-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111006973 PE=4 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 3.2e-53
Identity = 103/138 (74.64%), Postives = 121/138 (87.68%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           MENL  SL I+L SF  +A+ ILFYRHRAQFAYPN PPGS  YPIIGETLEM ++E+KGQ
Sbjct: 1   MENLFLSLLILLVSFPCVAVLILFYRHRAQFAYPNTPPGSIGYPIIGETLEMVAHEQKGQ 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PENFIFGR A++KT+VFTTSV+G+PTAAFC AAGNKLLFSNEQKLVTPWWP SA+KVFPS
Sbjct: 61  PENFIFGRVARHKTEVFTTSVLGEPTAAFCTAAGNKLLFSNEQKLVTPWWPESANKVFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
           T+K ++++EA+R KS LP
Sbjct: 121 TIKINTNDEAKRTKSLLP 138

[Close]

BLAST of CmaCh10G003420 vs. ExPASy TrEMBL
Match: A0A6J1C2F1 (beta-amyrin 28-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111006822 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.6e-39
Identity = 87/138 (63.04%), Postives = 100/138 (72.46%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           MENL  S  ++L S +   L +LF RHRAQFAY NIPPG+  YPI GE+LEMA+  KKG+
Sbjct: 1   MENLLLSAVVVLVSCVCGGLLLLFRRHRAQFAYRNIPPGTSGYPIFGESLEMAATRKKGE 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PE F F R AKYKT+VFTTS++G+P AAFC AAGNKLLFSNE KLVTPWWP SA KVFPS
Sbjct: 61  PEKFFFDRTAKYKTEVFTTSIVGEPAAAFCSAAGNKLLFSNEGKLVTPWWPESAAKVFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
               +  E    I S LP
Sbjct: 121 APGPADQEAVETI-SLLP 137

[Close]

BLAST of CmaCh10G003420 vs. ExPASy TrEMBL
Match: A0A7N2MUU5 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.4e-37
Identity = 76/137 (55.47%), Postives = 100/137 (72.99%), Query Frame = 0


Query: 2   ENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQP 61
           +N   SL ++  SF++L+LF LFYRHR+ F  PN+PPG   YP+IGE+ E  S   KG P
Sbjct: 3   QNFYLSLLLLFVSFITLSLFFLFYRHRSNFTGPNLPPGKVGYPMIGESFEFFSTGWKGHP 62

Query: 62  ENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPST 121
           E FIF R +KY ++VF TS++G+P A FC AAGNK LFSNE KLVT WWP S +KVFPS+
Sbjct: 63  EKFIFDRMSKYSSEVFKTSLLGQPAAVFCGAAGNKFLFSNENKLVTAWWPDSVNKVFPSS 122

Query: 122 VKYSSSEEARRIKSFLP 139
           ++ SS EEA++++  LP
Sbjct: 123 LQTSSKEEAKKMRKMLP 139

[Close]

BLAST of CmaCh10G003420 vs. ExPASy TrEMBL
Match: A0A061GRY3 (Cytochrome P450 OS=Theobroma cacao OX=3641 GN=TCM_039935 PE=3 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.9e-37
Identity = 74/138 (53.62%), Postives = 103/138 (74.64%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           ME++  SL ++  SF++L+LF+LFYRH++ ++ PN+PPG    PI+GE+LE  S   KG 
Sbjct: 1   MEHIYLSLLLLFVSFVTLSLFVLFYRHKSNYSAPNLPPGKPGLPIVGESLEFLSTGWKGH 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PE FIF R AKY + VF T+++G+P A FC AAGNK LFSNE KLVT WWP S DK+FPS
Sbjct: 61  PEKFIFDRIAKYSSQVFKTNILGEPAAVFCGAAGNKFLFSNENKLVTAWWPSSVDKIFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
           +++ SS EE+++++  LP
Sbjct: 121 SLQTSSKEESKKMRKLLP 138

[Close]

BLAST of CmaCh10G003420 vs. ExPASy TrEMBL
Match: A0A5D3CWG5 (Beta-amyrin 28-oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1610G00020 PE=4 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.9e-37
Identity = 79/138 (57.25%), Postives = 98/138 (71.01%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           ME+   SL I+L  FLSL LFILF+ H++ F+YPN PPG+   PI+GE+LE  S   +G 
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PE FIF R  KYK+DVF TS++G P A FC    NK LFSNE KLVTPWWP S +K+FPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
           T + SS EEA+++K  LP
Sbjct: 121 TTQTSSKEEAKKLKKLLP 138

[Close]

BLAST of CmaCh10G003420 vs. NCBI nr
Match: XP_038878724.1 (beta-amyrin 28-monooxygenase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 228.8 bits (582), Expect = 2.9e-56
Identity = 105/138 (76.09%), Postives = 124/138 (89.86%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           MEN+C S+ I+L  FLSLALFILFYRH+AQFAYPNIPPGS  YPIIGETL+M ++E+ GQ
Sbjct: 1   MENMCISVLILLILFLSLALFILFYRHKAQFAYPNIPPGSIGYPIIGETLQMVAHERNGQ 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PENF+FGR AKYKT++FTTS++G+PTAA C AAGNK LFSNEQKLVTPWWP SADKVFPS
Sbjct: 61  PENFMFGRVAKYKTEIFTTSILGEPTAALCSAAGNKFLFSNEQKLVTPWWPKSADKVFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
           TVKY+S+EEA+R+K+ LP
Sbjct: 121 TVKYNSNEEAKRMKNLLP 138

[Close]

BLAST of CmaCh10G003420 vs. NCBI nr
Match: XP_038878723.1 (beta-amyrin 28-monooxygenase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 228.8 bits (582), Expect = 2.9e-56
Identity = 105/138 (76.09%), Postives = 124/138 (89.86%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           MEN+C S+ I+L  FLSLALFILFYRH+AQFAYPNIPPGS  YPIIGETL+M ++E+ GQ
Sbjct: 1   MENMCISVLILLILFLSLALFILFYRHKAQFAYPNIPPGSIGYPIIGETLQMVAHERNGQ 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PENF+FGR AKYKT++FTTS++G+PTAA C AAGNK LFSNEQKLVTPWWP SADKVFPS
Sbjct: 61  PENFMFGRVAKYKTEIFTTSILGEPTAALCSAAGNKFLFSNEQKLVTPWWPKSADKVFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
           TVKY+S+EEA+R+K+ LP
Sbjct: 121 TVKYNSNEEAKRMKNLLP 138

[Close]

BLAST of CmaCh10G003420 vs. NCBI nr
Match: XP_022134788.1 (beta-amyrin 28-oxidase-like [Momordica charantia])

HSP 1 Score: 217.6 bits (553), Expect = 6.6e-53
Identity = 103/138 (74.64%), Postives = 121/138 (87.68%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           MENL  SL I+L SF  +A+ ILFYRHRAQFAYPN PPGS  YPIIGETLEM ++E+KGQ
Sbjct: 1   MENLFLSLLILLVSFPCVAVLILFYRHRAQFAYPNTPPGSIGYPIIGETLEMVAHEQKGQ 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PENFIFGR A++KT+VFTTSV+G+PTAAFC AAGNKLLFSNEQKLVTPWWP SA+KVFPS
Sbjct: 61  PENFIFGRVARHKTEVFTTSVLGEPTAAFCTAAGNKLLFSNEQKLVTPWWPESANKVFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
           T+K ++++EA+R KS LP
Sbjct: 121 TIKINTNDEAKRTKSLLP 138

[Close]

BLAST of CmaCh10G003420 vs. NCBI nr
Match: XP_022134593.1 (beta-amyrin 28-oxidase-like [Momordica charantia])

HSP 1 Score: 171.4 bits (433), Expect = 5.4e-39
Identity = 87/138 (63.04%), Postives = 100/138 (72.46%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           MENL  S  ++L S +   L +LF RHRAQFAY NIPPG+  YPI GE+LEMA+  KKG+
Sbjct: 1   MENLLLSAVVVLVSCVCGGLLLLFRRHRAQFAYRNIPPGTSGYPIFGESLEMAATRKKGE 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PE F F R AKYKT+VFTTS++G+P AAFC AAGNKLLFSNE KLVTPWWP SA KVFPS
Sbjct: 61  PEKFFFDRTAKYKTEVFTTSIVGEPAAAFCSAAGNKLLFSNEGKLVTPWWPESAAKVFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
               +  E    I S LP
Sbjct: 121 APGPADQEAVETI-SLLP 137

[Close]

BLAST of CmaCh10G003420 vs. NCBI nr
Match: XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])

HSP 1 Score: 170.2 bits (430), Expect = 1.2e-38
Identity = 80/138 (57.97%), Postives = 101/138 (73.19%), Query Frame = 0


Query: 1   MENLCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQ 60
           ME+   SL I    FLSL LFILF+ H++ F+YPN+PPG+   PI+GE+LE  S   KG+
Sbjct: 1   MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60

Query: 61  PENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 120
           PE FIF R  KYK+DVF TS++G P A FC +A NK LFSNE KLVTPWWP S +K+FPS
Sbjct: 61  PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 TVKYSSSEEARRIKSFLP 139
           + + SS EEA+++K FLP
Sbjct: 121 STQTSSKEEAKKLKKFLP 138

[Close]

BLAST of CmaCh10G003420 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 120.2 bits (300), Expect = 1.3e-27
Identity = 57/133 (42.86%), Postives = 85/133 (63.91%), Query Frame = 0


Query: 8   LFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQPENFIFG 67
           + I+  S + L+L +L  +H + F+YPN+PPG+   P+IGE+    S  ++G PE FI  
Sbjct: 6   MIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKFITD 65

Query: 68  RAAKYKTD---VFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPSTVKY 127
           R  ++ +    VF T + G PTA    A+GNK LF+NE KLV  WWP S +K+FPS+++ 
Sbjct: 66  RVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPSSMQT 125

Query: 128 SSSEEARRIKSFL 138
           SS EEAR+++  L
Sbjct: 126 SSKEEARKLRMLL 138

[Close]

BLAST of CmaCh10G003420 vs. TAIR 10
Match: AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )

HSP 1 Score: 116.7 bits (291), Expect = 1.5e-26
Identity = 55/130 (42.31%), Postives = 80/130 (61.54%), Query Frame = 0


Query: 8   LFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQPENFIFG 67
           +F+ ++S +   LF L  +H + F YPN+PPG   +P+IGETL   S  ++G PE F+  
Sbjct: 6   IFLFISSIIFPLLFFL-GKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFVTD 65

Query: 68  RAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPSTVKYSSS 127
           R   + + +F T + G P A    A+GNK LF+NE KLV  WWP S +K+FPS+ + SS 
Sbjct: 66  RVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTSSK 125

Query: 128 EEARRIKSFL 138
           EEA + +  L
Sbjct: 126 EEAIKTRMLL 134

[Close]

BLAST of CmaCh10G003420 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 3.7e-09
Identity = 38/110 (34.55%), Postives = 56/110 (50.91%), Query Frame = 0


Query: 4   LCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQPEN 63
           + F+ F++L S  S+A   L    R ++    +PPGS   P+IGET ++    K   PE 
Sbjct: 1   MAFTAFLLLLS--SIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEP 60

Query: 64  FIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPS 114
           FI  R A+Y + VF T + G+PT        N+ +  NE KL    +P S
Sbjct: 61  FIDERVARYGS-VFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPAS 107

[Close]

BLAST of CmaCh10G003420 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 3.7e-09
Identity = 38/110 (34.55%), Postives = 56/110 (50.91%), Query Frame = 0


Query: 4   LCFSLFIILASFLSLALFILFYRHRAQFAYPNIPPGSFWYPIIGETLEMASYEKKGQPEN 63
           + F+ F++L S  S+A   L    R ++    +PPGS   P+IGET ++    K   PE 
Sbjct: 1   MAFTAFLLLLS--SIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEP 60

Query: 64  FIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPS 114
           FI  R A+Y + VF T + G+PT        N+ +  NE KL    +P S
Sbjct: 61  FIDERVARYGS-VFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPAS 107

[Close]

BLAST of CmaCh10G003420 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 51.2 bits (121), Expect = 7.6e-07
Identity = 38/133 (28.57%), Postives = 63/133 (47.37%), Query Frame = 0


Query: 8   LFIILASFLSLALFILFYRHRAQFAYPNIP------PGSFWYPIIGETLEMASYEKKGQP 67
           +F+ +A+ +S  LF    +H  +F    I       PG    P IGET++    +K  + 
Sbjct: 12  VFLCIAATISSTLFFFRKKHH-RFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRV 71

Query: 68  -ENFIFGRAAKYKTDVFTTSVIGKPTAAFCLAAGNKLLFSNEQKLVTPWWPPSADKVFPS 127
            E+F+  R  K+  ++F T ++G PT     A  N+L+ SNE  LV   WP S+ ++   
Sbjct: 72  FEDFVNPRIIKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGM 131

Query: 128 TVKYSSSEEARRI 134
               +   E  R+
Sbjct: 132 NCIMAKQGEKHRV 142

The following BLAST results are available for this feature:

BLAST of CmaCh10G003420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6H9N6	4.5e-36	52.17	Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1	[more]
A5BFI4	1.3e-35	51.45	Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2	[more]
Q2MJ20	6.3e-30	47.45	Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV...	[more]
A0A0S2II38	3.1e-29	50.38	Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=...	[more]
I7C6E8	3.4e-28	47.86	Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1	[more]

BLAST of CmaCh10G003420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C307	3.2e-53	74.64	beta-amyrin 28-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111006973 PE=4 ...	[more]
A0A6J1C2F1	2.6e-39	63.04	beta-amyrin 28-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111006822 PE=3 ...	[more]
A0A7N2MUU5	1.4e-37	55.47	Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]
A0A061GRY3	1.9e-37	53.62	Cytochrome P450 OS=Theobroma cacao OX=3641 GN=TCM_039935 PE=3 SV=1	[more]
A0A5D3CWG5	1.9e-37	57.25	Beta-amyrin 28-oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1...	[more]

BLAST of CmaCh10G003420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878724.1	2.9e-56	76.09	beta-amyrin 28-monooxygenase-like isoform X2 [Benincasa hispida]	[more]
XP_038878723.1	2.9e-56	76.09	beta-amyrin 28-monooxygenase-like isoform X1 [Benincasa hispida]	[more]
XP_022134788.1	6.6e-53	74.64	beta-amyrin 28-oxidase-like [Momordica charantia]	[more]
XP_022134593.1	5.4e-39	63.04	beta-amyrin 28-oxidase-like [Momordica charantia]	[more]
XP_038877759.1	1.2e-38	57.97	LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida]	[more]

BLAST of CmaCh10G003420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36110.1	1.3e-27	42.86	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT5G36140.1	1.5e-26	42.31	cytochrome P450, family 716, subfamily A, polypeptide 2	[more]
AT5G05690.1	3.7e-09	34.55	Cytochrome P450 superfamily protein	[more]
AT5G05690.2	3.7e-09	34.55	Cytochrome P450 superfamily protein	[more]
AT2G42850.1	7.6e-07	28.57	cytochrome P450, family 718	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 26..138 e-value: 1.1E-12 score: 49.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 29..137
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 9..138
None	No IPR available	PANTHER	PTHR24286:SF297	BETA-AMYRIN 28-OXIDASE-LIKE	coord: 9..138

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh10G003420.1	CmaCh10G003420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen