CmaCh09G010730 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh09G010730
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like
LocationCma_Chr09: 5643156 .. 5648590 (+)
RNA-Seq ExpressionCmaCh09G010730
SyntenyCmaCh09G010730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAGCATTGAAGCAGCCCCTACCATACCAACTCACCCGATAAGGATCAAACCCAATCCAAAAACGACACTCTCCAACTCACCACCTTCTTCATTCTTCTACAAATTTCCACTTTCAAATCATTTTGAACTCCTCCTCCTCCTCCTCCTCTTTCTCTCCAATTTTTCTTTCATCGCTATGATTCCGTTCGTACGATCGTCGTCTCGAAATTTACTCGCCCTTCTCAACCAAAACTATCTCTACCCATCTTTACTCTGTTCTGTTTCGGAATCTCCTATATTAGTTCGGTACGATGGTCGCTGGACTGGATCTCTTTGGTCAACTCCTCGATTTGAATCCACAGAAGCAGTGCGACGTGTAGATCAACTGTATGTTCATGACAACGATGATCAGGTTTGGTGGCTGTTTATTAGTATTTTGAACGTTTTCTCTATCATGCATGTGTGTGTGTGTTTTTTTTTCTATTTAGTTAAAAAAATTGTTAATACTTTATTTAAAAAATATTTTTAGTGGGTTTCAAAAATATTATCCAACTTGGAATAAATTAACTTTATATTTTTTTATTATTTAATTGAAGAAATTTTAAAAAATTATTTATGGAATAAATTAAAAGTGATTCGATAAAATCCGAAACAAAATGTTCCTAAAGTTCGAGATTGAACAATGTTGTATATTTTAAAGGGTAAAAAAAACTCTTAAACTTAAAAAAATAAATAATTAAATTTTAAACCTTAATCAAACACTAATTAATCCAAAATTAATAATTAAACTCGTTTTTATCTAAATTTGATTAATTATTTAACAATTTATCTATAAAAATAAATTTTTATTTTTTTGAATTTAATAAGTAACTTAAGTTAGAGAAATTTTGTGGGACACCTCGAAATTAATTAGTAACTTAAGCAACCGAAATAAAATTCATAATAAACTTACAAGTCCGGTAAGGTAAGATTGAGTTGTAACTCTATTTTTTGATTGGTCGTGTTGACTTAACAAAAAAATGTCAACTCATGAACCGACTCAAAATTTAACACGATTCAATCCTCACCAAAAAAATAAAAAAAAAAATCAACCTAACTCGACTTTTATGATTAGAGTTAGGTGGTCCGAATTAATCAGATATCCGAGTCATATCAATATCCCTAATTCTAATTTTTTTTTTTTTTAATTTTCTCTTCAAAACTTTTTATCTCTCTCTATCTATCTATATATCTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATTATTCTTTGTAAAGGAATTTGCCCACTTTTCTTAATACTTTTCTCTAAATATCTTTATTAAACTTTATTCTCTCTATGAAGTATTTATCTTATATATCTTTTTAAACCCAAACATGTTTTTTTTTATTTATAATTTTTATCTCTCTCTACCATTTTTACTCAATATTTCCCCTATTTTTTAATATTTATATATATATATATATAGAGGATAATATTTAATATTAAAAGCTTGTCATATCATATAAATAATGTCACCATAATTAATATGATAGCACATGGATAATATTTAATATAAAATAGTTGTCGAATCATATAAAAATGTCGATAACAATGGATAAAAAAAATAATAAAAATAAAGAATATATATTTCATTAATAAGTTAAAATTATTATTTATCTTATATTTATTATAATAACGTTTTTTTTTTTTTTTTTTTACATTTTATCCATTTTTTTATCATATAATGAAAATATTGATTCAATTTTATATATGTCGATATGAACATATAAAAATGAATGAGAAATAAAAAATTATTAAATATTAAAGATTTTTGGATTTTTTATTTTTATTTTTTATTTTACATTTTTATTATTTATTATAAGACTAAATAGCTTTTATTTGAAATGACTTTTTTAATTATTTAAATTAAGAAACTATTTATTGTTGTAGAAGTTTTCTTAAAAAGAAAATATTTAGTGTTATAGGAATTGGATTTCCCGGGAGGAAAAGTCCCATTTACGAGTGAAATGAGATTCATTGGTGAGTCGACGATTCAGAAGCGAGTTCCGTGTTATCGTGTTATTGATGAAATTGGAGAGATAACTACATCGAATAATTTCACGCAGGTATTATTCTCATTTATTTATTTATTTATATATTCCAATTTTCATCTGTGATATTCTACATTGGTTTGTGAGGATAACAAGTGAAAATCTTCCCAAGCGTTTTAAAGGCTTGAGGGGAAGGCTGAAAGTAAAAATCCAAAGAGGACAATATCTACTCACAGTGGATCTGGGCCGTTACAAATGGTATCAGAACCAGACATCAGACGATGTGCTAGCCTCCTCGCTATTCCCCGAAGGGGGTAGACACAAGGCAGTGTGCCAGTAAGGACACTAGGCCTCAAAGGGGGGGGGGGGGATTTGGGGCGGTCCCACATCGATTGGAGGAAGGAAAGAGTGTCAGCGAGGGCTGGGCCTGAAAGGGGTGGTTGTGATGTTCCACATTGGTTGGAAAGAGAACAAAACACCCTTTATAAGGGTGTGGAAATCTTACATTGGCAGACACGTTTTGAGGGGAAGTCCAAAAAGGACAATATTTGCTAGCGGTGGATTTGGGCCGTTACAACATCCCTAATATTTTTTTGTTCTTGATAGTTGAGAAAAGATGAAGCGATTAAGATGTACAAAGACATGATAACCCTTCAAACAATGGACACCATATTTTACGAAGCACAAAGGCAAGGACGAATCTCATTTTACTTAACTTCATCCGGAGAAGAAGCTATTTCAGTTGCTTCCGCAGCTGCCCTTCATCCCGACGACGTTGCATTAGCTCAGGTAAATGAAGATCGAGAGACTCAAACATTTGATTGCTTAACGAAAGACATGCATTAATCATTTGAACTATGCTCAAGTTGATTAACTGTGAATTGGGTTGACAGTATAGAGAGCCGGGAGTGTTGGTATGGCGTGGGTTTACGTTGCAAGAATTTGCTAACCAGTTGTTTGGAAACAAAGACGATTATGGGAAGGGTCGACAAATGCCCATTCACTATGGCTCGAAGCAACACAATTACTTCACCATTTCATCGCCTCTTGGGTAACTTTTTCCGTTCTATAAACACTAATCTCTGTTTACGACAATGATTTGAACGTAACGCAATGGAACATTAAGAAAAGTAGATGGTTTGTTTAGAAGTAAATAGTAAATGGATTTCTTTCTTTATGACGTTTTGATATTGAAGAACACAAATTCCACAAGCGGTTGGCATTGCTCATTCGCTTAAAATGGACAAACAAGATGCTTGTGCTGTAGCTTATTTTGGAGATGGCACCACCAGCGAGGTACGGTTTAGTCACTACTGTTCCGATATTTTCTTCTTAGATAAACCTTTCTAATCTATACTAAAAAAATATATATATTGAATTGGATTTACAGGGCGATTTCCACGCGGGGTTGAACTTGGCAGCCGTATTAGGAGCGCCGGTGATTTTCATCTGCCGCAACAACGGCTGGGCTATCAGCACTTCCGTCCAAGAACAATTCCGAAGTACCAGATCCCCAAATTCCGTTCAAAATCCATAATCTCACGCTCTCAATTCGAAGCCCTAATTAATATCTTGGCGTTGATTTTGCAGGCGATGGCGTGGTAGTGAAAGGTCAGGGCTATGGAATACGAAGCATACGAATCGATGGAAACGATGCCCTAGCCATTTACAGTGCGGTTCGCAAAGCTAGATCCATGGCGGTTGCAGAGAAAATGCCGGTTCTGATCGAGGCTTTGACTTACAGAGTTGGCCACCATTCTACCTCCGATGATTCCAGTAAGTACCGCGCTGTTGATGAGATTGAGCACTGGAAAATGGAGAGGAACCCTGTGAATCGCTTTGCTAAATGGCTTACCAATAATGGATGGTTGAGTGTAGAAGATGAGTACAAGCATAAAACCACCATTAAAAAACAGGTCAGTTTTTTGCTTTCGCTTTTGTTAGAATGTTTGAATTGTTAACTAAATTCTAGGAACAAAAACAAAGATTTGTAAATAACAATACAGGAAACTTAAGAGAATTAGTATACTTTTGTTATAAACTTCTTAGTTTGGATGAATTCTTGGGTCTGGTAATCAATGAGTTCATGCCAGCCATGCTTCAACGTTCAAGCATACCGCTAGTGGACATTGTCCCATTGTCTTTTTTGCAGACATTGTTTGAGTTTTTTCATTTGAGCTTCCCTTCAAAGTTTTTAAAACGCTTAGGTTAGAAAGAGATTTCCACAACCTTGTAAAATGCTTCCTTCCCCTCCCCGGCCAATGTGGGATCTCACAATCCACCTCTTCGAAGTCCAGCATCCTCATTGACAATCGTTCCCTTCTCCAATTCATGTGAGTCCTTCCAATCCACCCTCTTTGAGGCCTTCCACACCCATATAAAAAATGTTTTGTTCCCCTCTCCAACTAACATGGAATCTCACAATCCACTCCATTTGATACTCAGCGTCCTTGCTGACACTCGTTCCCTTCTCCCATTGATGTAGGACACTCTAAATCACCTCCTTCGAGACCCAGCATCCTTGTTGGCACACAGCCTCTTGTCCACCCCACACCTTTGTAAAGAATGTTTCGTTCCCCCCCCCCTCCAATCGATGTGGGATCTCACAATTCACCCTTCTCAAAACCCAGCATCCTCGCTGACACTCGTTCCCTTCTCCAATCGATGTGGGACCCTCCAATCCACCCCCCTTCGGGACCTAACATCCTTACTAACATATCACCTCGTGTCCACCCCCTTGGTTCTCAACCTCCTCACTGGCATATTGCCCATCTGGCTCTGATATCATTTGTAATGACCTAAACCCACTGCTAGCATATATTGTCTTCTTTGGACTTTCTGTTTTGTGCTTCCCCTTCGTGATGTAGTTGGTTATCACGTTAGTCTTACTTACTAAAGGTCTCTTGTTCAAGTTTAGGCGACGTCATATTTGAAATTTTTATTTTTATTTTATTTTATTTTATAATCTCGATTCATTTTATACCTCTACAAGAAAATGTAAGAGCTTTAGAGCTTGATAAGTTGAGAATCGTGCTCTCCAACACACAATGAGCATGGGCTTTTAAAACACGTCTATTAGAAAGAGGTTTTCACACTCTTATAAAGAATGATTCGTTCTCCTCTCCAACCAATGTGATGAATGCTATTGTTTGATTGAATAAATTAAATTTCAAGGTGCTGAATGTGCTAAAGCTACCCATTTTGTTGGTTCAGTTGTTACAAGCAATCCAGAATGCAGAGAAGACAGAGAAGCCTCCACTTTCAGAGTTGTTCAATGATGTTTATGACCATTTACCCTCAAACCTTCAAGAACAAGAGGAAGTACTCAGGCAAACAACGAAGAGGTATCCTCAAGATTATCCTTCTGATGTTCCTCTTTAG

mRNA sequence

GAAGAGCATTGAAGCAGCCCCTACCATACCAACTCACCCGATAAGGATCAAACCCAATCCAAAAACGACACTCTCCAACTCACCACCTTCTTCATTCTTCTACAAATTTCCACTTTCAAATCATTTTGAACTCCTCCTCCTCCTCCTCCTCTTTCTCTCCAATTTTTCTTTCATCGCTATGATTCCGTTCGTACGATCGTCGTCTCGAAATTTACTCGCCCTTCTCAACCAAAACTATCTCTACCCATCTTTACTCTGTTCTGTTTCGGAATCTCCTATATTAGTTCGGTACGATGGTCGCTGGACTGGATCTCTTTGGTCAACTCCTCGATTTGAATCCACAGAAGCAGTGCGACGTGTAGATCAACTGTATGTTCATGACAACGATGATCAGGAATTGGATTTCCCGGGAGGAAAAGTCCCATTTACGAGTGAAATGAGATTCATTGGTGAGTCGACGATTCAGAAGCGAGTTCCGTGTTATCGTGTTATTGATGAAATTGGAGAGATAACTACATCGAATAATTTCACGCAGTTGAGAAAAGATGAAGCGATTAAGATGTACAAAGACATGATAACCCTTCAAACAATGGACACCATATTTTACGAAGCACAAAGGCAAGGACGAATCTCATTTTACTTAACTTCATCCGGAGAAGAAGCTATTTCAGTTGCTTCCGCAGCTGCCCTTCATCCCGACGACGTTGCATTAGCTCAGTATAGAGAGCCGGGAGTGTTGGTATGGCGTGGGTTTACGTTGCAAGAATTTGCTAACCAGTTGTTTGGAAACAAAGACGATTATGGGAAGGGTCGACAAATGCCCATTCACTATGGCTCGAAGCAACACAATTACTTCACCATTTCATCGCCTCTTGGAACACAAATTCCACAAGCGGTTGGCATTGCTCATTCGCTTAAAATGGACAAACAAGATGCTTGTGCTGTAGCTTATTTTGGAGATGGCACCACCAGCGAGGGCGATTTCCACGCGGGGTTGAACTTGGCAGCCGTATTAGGAGCGCCGGTGATTTTCATCTGCCGCAACAACGGCTGGGCTATCAGCACTTCCGTCCAAGAACAATTCCGAAGCGATGGCGTGGTAGTGAAAGGTCAGGGCTATGGAATACGAAGCATACGAATCGATGGAAACGATGCCCTAGCCATTTACAGTGCGGTTCGCAAAGCTAGATCCATGGCGGTTGCAGAGAAAATGCCGGTTCTGATCGAGGCTTTGACTTACAGAGTTGGCCACCATTCTACCTCCGATGATTCCAGTAAGTACCGCGCTGTTGATGAGATTGAGCACTGGAAAATGGAGAGGAACCCTGTGAATCGCTTTGCTAAATGGCTTACCAATAATGGATGGTTGAGTGTAGAAGATGAGTACAAGCATAAAACCACCATTAAAAAACAGAATGCAGAGAAGACAGAGAAGCCTCCACTTTCAGAGTTGTTCAATGATGTTTATGACCATTTACCCTCAAACCTTCAAGAACAAGAGGAAGTACTCAGGCAAACAACGAAGAGGTATCCTCAAGATTATCCTTCTGATGTTCCTCTTTAG

Coding sequence (CDS)

ATGATTCCGTTCGTACGATCGTCGTCTCGAAATTTACTCGCCCTTCTCAACCAAAACTATCTCTACCCATCTTTACTCTGTTCTGTTTCGGAATCTCCTATATTAGTTCGGTACGATGGTCGCTGGACTGGATCTCTTTGGTCAACTCCTCGATTTGAATCCACAGAAGCAGTGCGACGTGTAGATCAACTGTATGTTCATGACAACGATGATCAGGAATTGGATTTCCCGGGAGGAAAAGTCCCATTTACGAGTGAAATGAGATTCATTGGTGAGTCGACGATTCAGAAGCGAGTTCCGTGTTATCGTGTTATTGATGAAATTGGAGAGATAACTACATCGAATAATTTCACGCAGTTGAGAAAAGATGAAGCGATTAAGATGTACAAAGACATGATAACCCTTCAAACAATGGACACCATATTTTACGAAGCACAAAGGCAAGGACGAATCTCATTTTACTTAACTTCATCCGGAGAAGAAGCTATTTCAGTTGCTTCCGCAGCTGCCCTTCATCCCGACGACGTTGCATTAGCTCAGTATAGAGAGCCGGGAGTGTTGGTATGGCGTGGGTTTACGTTGCAAGAATTTGCTAACCAGTTGTTTGGAAACAAAGACGATTATGGGAAGGGTCGACAAATGCCCATTCACTATGGCTCGAAGCAACACAATTACTTCACCATTTCATCGCCTCTTGGAACACAAATTCCACAAGCGGTTGGCATTGCTCATTCGCTTAAAATGGACAAACAAGATGCTTGTGCTGTAGCTTATTTTGGAGATGGCACCACCAGCGAGGGCGATTTCCACGCGGGGTTGAACTTGGCAGCCGTATTAGGAGCGCCGGTGATTTTCATCTGCCGCAACAACGGCTGGGCTATCAGCACTTCCGTCCAAGAACAATTCCGAAGCGATGGCGTGGTAGTGAAAGGTCAGGGCTATGGAATACGAAGCATACGAATCGATGGAAACGATGCCCTAGCCATTTACAGTGCGGTTCGCAAAGCTAGATCCATGGCGGTTGCAGAGAAAATGCCGGTTCTGATCGAGGCTTTGACTTACAGAGTTGGCCACCATTCTACCTCCGATGATTCCAGTAAGTACCGCGCTGTTGATGAGATTGAGCACTGGAAAATGGAGAGGAACCCTGTGAATCGCTTTGCTAAATGGCTTACCAATAATGGATGGTTGAGTGTAGAAGATGAGTACAAGCATAAAACCACCATTAAAAAACAGAATGCAGAGAAGACAGAGAAGCCTCCACTTTCAGAGTTGTTCAATGATGTTTATGACCATTTACCCTCAAACCTTCAAGAACAAGAGGAAGTACTCAGGCAAACAACGAAGAGGTATCCTCAAGATTATCCTTCTGATGTTCCTCTTTAG

Protein sequence

MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRRVDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQLRKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQYREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAVGIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHSTSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKKQNAEKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL
Homology
BLAST of CmaCh09G010730 vs. ExPASy Swiss-Prot
Match: Q9LPL5 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g21400 PE=2 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 1.2e-162
Identity = 282/414 (68.12%), Postives = 331/414 (79.95%), Query Frame = 0

Query: 51  RFESTEAVRRVDQL--YVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEI 110
           R EST    + D L   + + D QELDFPGGKV +TSEM+FI ES+  +R+PCYRV+DE 
Sbjct: 55  RHESTAVETQADHLVQQIDEVDAQELDFPGGKVGYTSEMKFIPESS-SRRIPCYRVLDED 114

Query: 111 GEITTSNNFTQLRKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASA 170
           G I   ++F  + +  A++MY+ M TLQ MD IFYEAQRQGRISFYLTS GEEAI++ASA
Sbjct: 115 GRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASA 174

Query: 171 AALHPDDVALAQYREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTI 230
           AAL PDDV L QYREPGVL+WRGFTL+EFANQ FGNK DYGKGRQMPIHYGS + NYFTI
Sbjct: 175 AALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTI 234

Query: 231 SSPLGTQIPQAVGIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICR 290
           SSP+ TQ+PQA G+ +SLKMDK++AC V + GDG TSEGDFHAGLN AAV+ APV+FICR
Sbjct: 235 SSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICR 294

Query: 291 NNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVL 350
           NNGWAIST + EQFRSDG+VVKGQ YGIRSIR+DGNDALA+YSAVR AR MAV E+ PVL
Sbjct: 295 NNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVL 354

Query: 351 IEALTYRVGHHSTSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTT 410
           IE +TYRVGHHSTSDDS+KYRA DEI++WKM RNPVNRF KW+ +NGW S EDE K ++ 
Sbjct: 355 IEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSN 414

Query: 411 IKK------QNAEKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYP 457
            +K      Q AEK EK PL+ELFNDVYD  P NL+EQE  L++  K+ PQDYP
Sbjct: 415 ARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYP 467

BLAST of CmaCh09G010730 vs. ExPASy Swiss-Prot
Match: Q84JL2 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g09300 PE=1 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.3e-156
Identity = 279/464 (60.13%), Postives = 345/464 (74.35%), Query Frame = 0

Query: 7   SSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRRVDQLYV 66
           SS   LL +L  N  + S          ++ +D    GS     R  +T A       +V
Sbjct: 10  SSKSTLLNILRHNLGFGSRSHVTRHIRQILPHDPPLRGSQNPISRLCNTMAEPETLSSFV 69

Query: 67  ---HDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQLRKD 126
              + N+ Q +DFPGGKV FT E++FI ES  ++RVPCYRV+D+ G++ T++ F Q+ ++
Sbjct: 70  QHEYANNHQVMDFPGGKVAFTPEIQFISESD-KERVPCYRVLDDNGQLITNSQFVQVSEE 129

Query: 127 EAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQYRE 186
            A+K+Y DM+TLQ MD IFYEAQRQGR+SFY T+ GEEAI++ASAAAL P DV   QYRE
Sbjct: 130 VAVKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYRE 189

Query: 187 PGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAVGIA 246
           PGVL+WRGFTLQEFANQ FGNK DYGKGRQMP+HYGS + NYFT+S+ + TQ+P AVG A
Sbjct: 190 PGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAA 249

Query: 247 HSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQEQFR 306
           +SLKMDK+DACAV YFGDG TSEGDFHA LN+AAV+ APV+FICRNNGWAIST   +QFR
Sbjct: 250 YSLKMDKKDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFR 309

Query: 307 SDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHSTSD 366
           SDGVVVKG+ YGIRSIR+DGNDALA+YSAV  AR MA+ E+ P+LIEALTYRVGHHSTSD
Sbjct: 310 SDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSD 369

Query: 367 DSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKKQN------AEKT 426
           DS++YR+  EIE W   RNP++RF  W+ +NGW S + E   ++ IKK+       AEKT
Sbjct: 370 DSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKT 429

Query: 427 EKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           EKP L  +F+DVYD  PSNL+EQE ++RQT   +PQDYPSDVPL
Sbjct: 430 EKPNLQNMFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

BLAST of CmaCh09G010730 vs. ExPASy Swiss-Prot
Match: P50136 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=10090 GN=Bckdha PE=1 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 7.8e-109
Identity = 192/394 (48.73%), Postives = 259/394 (65.74%), Query Frame = 0

Query: 71  DQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQLRKDEAIKMYK 130
           D +  FPG    F  ++ FI  + I   +P YRV+D  G+I   +    L ++E +K Y+
Sbjct: 46  DDKPQFPGASAEFVDKLEFIQPNVI-SGIPIYRVMDRQGQIINPSEDPHLPQEEVLKFYR 105

Query: 131 DMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQYREPGVLVWR 190
            M  L TMD I YE+QR+GRISFY+T+ GEE   V SAAAL   D+   QYRE GVL++R
Sbjct: 106 SMTLLNTMDRILYESQREGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYR 165

Query: 191 GFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAVGIAHSLKMDK 250
            + L+ F +Q +GN +D GKGRQMP+HYG K+ ++ TISSPL TQIPQAVG A++ K   
Sbjct: 166 DYPLELFMSQCYGNVNDPGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRAN 225

Query: 251 QDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVK 310
            +   + YFG+G  SEGD HAG N AA L  P+IF CRNNG+AIST   EQ+R DG+  +
Sbjct: 226 ANRIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAAR 285

Query: 311 GQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHSTSDDSSKYRA 370
           G GYGI+SIR+DGND  A+Y+A ++AR  AVAE  P LIEA+TYR+GHHSTSDDSS YR+
Sbjct: 286 GPGYGIKSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRS 345

Query: 371 VDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QNAEKTEKPPLSE 430
           VDE+ +W  + +P++R  ++L N GW   E E   +   +K      + AE+  KP  S 
Sbjct: 346 VDEVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSL 405

Query: 431 LFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSD 459
           LF+DVY  +P+ L+ Q+E L +  + Y + YP D
Sbjct: 406 LFSDVYQEMPAQLRRQQESLARHLQTYGEHYPLD 438

BLAST of CmaCh09G010730 vs. ExPASy Swiss-Prot
Match: P11960 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (Fragment) OS=Rattus norvegicus OX=10116 GN=Bckdha PE=1 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.3e-108
Identity = 193/394 (48.98%), Postives = 257/394 (65.23%), Query Frame = 0

Query: 71  DQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQLRKDEAIKMYK 130
           D +  FPG    F  ++ FI  + I   +P YRV+D  G+I   +    L ++E +K+Y+
Sbjct: 45  DDKPQFPGASAEFVDKLEFIQPNVI-SGIPIYRVMDRQGQIINPSEDPHLPQEEVLKLYR 104

Query: 131 DMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQYREPGVLVWR 190
            M  L TMD I YE+QRQGRISFY+T+ GEE   V SAAAL   D+   QYRE GVL++R
Sbjct: 105 SMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYR 164

Query: 191 GFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAVGIAHSLKMDK 250
            + L+ F  Q +GN  D GKGRQMP+HYG K+ ++ TISSPL TQIPQAVG A++ K   
Sbjct: 165 DYPLELFMAQCYGNVSDPGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRAN 224

Query: 251 QDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVK 310
            +   + YFG+G  SEGD HAG N AA L  P+IF CRNNG+AIST   EQ+R DG+  +
Sbjct: 225 ANQIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAAR 284

Query: 311 GQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHSTSDDSSKYRA 370
           G GYGI SIR+DGND  A+Y+A ++AR  AVAE  P LIEA+TYR+GHHSTSDDSS YR+
Sbjct: 285 GPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRS 344

Query: 371 VDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QNAEKTEKPPLSE 430
           VDE+ +W  + +P++R  ++L N GW   E E   +   +K      + AE+  KP  S 
Sbjct: 345 VDEVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSL 404

Query: 431 LFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSD 459
           LF+DVY  +P+ L+ Q+E L +  + Y + YP D
Sbjct: 405 LFSDVYQEMPAQLRRQQESLARHLQTYGEHYPLD 437

BLAST of CmaCh09G010730 vs. ExPASy Swiss-Prot
Match: A5A6H9 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes OX=9598 GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 3.9e-108
Identity = 193/394 (48.98%), Postives = 257/394 (65.23%), Query Frame = 0

Query: 71  DQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQLRKDEAIKMYK 130
           D +  FPG    F  ++ FI  + I   +P YRV+D  G+I   +    L K++ +K+YK
Sbjct: 49  DDKPQFPGASAEFIDKLEFIQPNVI-SGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYK 108

Query: 131 DMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQYREPGVLVWR 190
            M  L TMD I YE+QRQGRISFY+T+ GEE   V SAAAL   D+   QYRE GVL++R
Sbjct: 109 SMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYR 168

Query: 191 GFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAVGIAHSLKMDK 250
            + L+ F  Q +GN  D GKGRQMP+HYG K+ ++ TISSPL TQIPQAVG A++ K   
Sbjct: 169 DYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRAN 228

Query: 251 QDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVK 310
            +   + YFG+G  SEGD HAG N AA L  P+IF CRNNG+AIST   EQ+R DG+  +
Sbjct: 229 ANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAAR 288

Query: 311 GQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHSTSDDSSKYRA 370
           G GYGI SIR+DGND  A+Y+A ++AR  AVAE  P LIEA+TYR+GHHSTSDDSS YR+
Sbjct: 289 GPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRS 348

Query: 371 VDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QNAEKTEKPPLSE 430
           VDE+ +W  + +PV+R   +L + GW   E E   +   +K      + AE+  KP  + 
Sbjct: 349 VDEVNYWDKQDHPVSRLRHYLLSQGWWDEEQEKAWRKQSRKKVMEAFEQAERKPKPNPNL 408

Query: 431 LFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSD 459
           LF+DVY  +P+ L++Q+E L +  + Y + YP D
Sbjct: 409 LFSDVYQEMPAQLRKQQESLARHLQTYGEHYPLD 441

BLAST of CmaCh09G010730 vs. ExPASy TrEMBL
Match: A0A6J1IJ53 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111473857 PE=4 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 4.2e-267
Identity = 461/467 (98.72%), Postives = 461/467 (98.72%), Query Frame = 0

Query: 1   MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR 60
           MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR
Sbjct: 1   MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR 60

Query: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL 120
           VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL
Sbjct: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL 120

Query: 121 RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ 180
           RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ
Sbjct: 121 RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ 180

Query: 181 YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV 240
           YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV
Sbjct: 181 YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV 240

Query: 241 GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE 300
           GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE
Sbjct: 241 GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE 300

Query: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS 360
           QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS
Sbjct: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS 360

Query: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QNA 420
           TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK      QNA
Sbjct: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKKQLLQAIQNA 420

Query: 421 EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL
Sbjct: 421 EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 467

BLAST of CmaCh09G010730 vs. ExPASy TrEMBL
Match: A0A6J1FEF0 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111443206 PE=4 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 3.6e-250
Identity = 434/467 (92.93%), Postives = 444/467 (95.07%), Query Frame = 0

Query: 1   MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR 60
           MIPFVRSSSRNLLALLNQN+ Y SLLCSVSESP+LVR   RWTGSLW TPRFESTEAVR 
Sbjct: 1   MIPFVRSSSRNLLALLNQNHRYLSLLCSVSESPVLVRCGARWTGSLWFTPRFESTEAVRD 60

Query: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL 120
           VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGES  QKRVPCYRVIDEIGE TTSNNFTQL
Sbjct: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESATQKRVPCYRVIDEIGETTTSNNFTQL 120

Query: 121 RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ 180
            KD AIKMYKDMITLQT+DTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDV LAQ
Sbjct: 121 SKDGAIKMYKDMITLQTVDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVVLAQ 180

Query: 181 YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV 240
           YREPGVL+WRGFTLQEFANQLFGNK DYGKGRQMPIHYGS QHNYFTISSPLGTQIPQAV
Sbjct: 181 YREPGVLLWRGFTLQEFANQLFGNKSDYGKGRQMPIHYGSIQHNYFTISSPLGTQIPQAV 240

Query: 241 GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE 300
           GIA+SLKMDK+DACAVAYFGDGTTSEGDFHAGLN AAVLGAPVIFICRNNGWAISTSVQE
Sbjct: 241 GIAYSLKMDKKDACAVAYFGDGTTSEGDFHAGLNFAAVLGAPVIFICRNNGWAISTSVQE 300

Query: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS 360
           QFRSDGVVVKGQGYGIRSIRIDGNDALA+YSAVR+ARSMAVAE+MPVLIEALTYRVGHHS
Sbjct: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAVYSAVRRARSMAVAEQMPVLIEALTYRVGHHS 360

Query: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QNA 420
           TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDE KHKTTIKK      QNA
Sbjct: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDESKHKTTIKKQLLQAIQNA 420

Query: 421 EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           EKTEKPPLSELF+DVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL
Sbjct: 421 EKTEKPPLSELFSDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 467

BLAST of CmaCh09G010730 vs. ExPASy TrEMBL
Match: A0A1S3BD09 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103488700 PE=4 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.6e-210
Identity = 374/472 (79.24%), Postives = 406/472 (86.02%), Query Frame = 0

Query: 1   MIPFVRSSSRNLL-ALLNQNYLYPSLLCSVSESPILVRYDGR----WTGSLWSTPRFEST 60
           MI FVRSSS NL+  +LN+N+         S+S + +R   R     +G LW T RFEST
Sbjct: 1   MIRFVRSSSPNLIHRILNRNF-------HCSDSSMALRCGRRRKDYSSGFLWPTCRFEST 60

Query: 61  EAVRRVDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSN 120
           EA R VDQLYV ++DDQELDFPGG VPF +EMRFI EST +KRVPCYRV+ E G+I T N
Sbjct: 61  EAARHVDQLYVPNHDDQELDFPGGSVPFRNEMRFISEST-EKRVPCYRVLHETGDIITPN 120

Query: 121 NFTQLRKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDD 180
           NF QL KD AIKMYKDMITLQ MD IFYEAQRQGRISFYLTSSGEEAI+VASAAAL PDD
Sbjct: 121 NFNQLTKDVAIKMYKDMITLQIMDNIFYEAQRQGRISFYLTSSGEEAIAVASAAALLPDD 180

Query: 181 VALAQYREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQ 240
           V LAQYREPGVL+WRGFTLQEFANQLFGNK DYGKGRQMPIHYGS  HNYFTISSPLGTQ
Sbjct: 181 VVLAQYREPGVLLWRGFTLQEFANQLFGNKRDYGKGRQMPIHYGSNHHNYFTISSPLGTQ 240

Query: 241 IPQAVGIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAIS 300
           +PQAVGIA+SLKMDK+DACAVAYFGDGTTSEGDFHA LN AAVLGAPVIFICRNNGWAIS
Sbjct: 241 LPQAVGIAYSLKMDKKDACAVAYFGDGTTSEGDFHAALNFAAVLGAPVIFICRNNGWAIS 300

Query: 301 TSVQEQFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYR 360
           TS++EQFRSDGVVVKGQGYGIRSIRIDGNDALA+Y+AV KARSMA+ EKMP+LIEALTYR
Sbjct: 301 TSIEEQFRSDGVVVKGQGYGIRSIRIDGNDALAVYTAVHKARSMAIREKMPILIEALTYR 360

Query: 361 VGHHSTSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK---- 420
           VGHHSTSDDSSKYR  DEI++WKMERNPV+RFAKW++NNGW S EDE  HK T+KK    
Sbjct: 361 VGHHSTSDDSSKYRTTDEIQYWKMERNPVDRFAKWISNNGWFSQEDESNHKITVKKLLLE 420

Query: 421 --QNAEKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
             QNAEKTEKPPLSELFNDVYDHLP NLQEQEEVLRQT KR+PQDYPSDVPL
Sbjct: 421 AIQNAEKTEKPPLSELFNDVYDHLPKNLQEQEEVLRQTRKRHPQDYPSDVPL 464

BLAST of CmaCh09G010730 vs. ExPASy TrEMBL
Match: A0A0A0KIX5 (E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G395130 PE=4 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.7e-207
Identity = 367/470 (78.09%), Postives = 400/470 (85.11%), Query Frame = 0

Query: 1   MIPFVRSSSRNLL-ALLNQNYLYPSLLCSVSESPILV--RYDGRWTGSLWSTPRFESTEA 60
           MI F RSSSRNL   +LN+ + Y    CS S   +    R   + +G  W T RFESTEA
Sbjct: 1   MIRFARSSSRNLFHRVLNRKFQY----CSDSSMAVQCGRRRKDQSSGFFWPTCRFESTEA 60

Query: 61  VRRVDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNF 120
            R VDQLY+H++DDQELDFPGG VPFT EMRF+ EST +KRVPCYRV+ E G+  T  NF
Sbjct: 61  ARHVDQLYLHNHDDQELDFPGGSVPFTKEMRFMSEST-EKRVPCYRVLHENGDAITPPNF 120

Query: 121 TQLRKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVA 180
            QL KD AIKMYKDMITLQ MD IFYEAQRQGRISFYLTSSGEEAI++ASAAAL PDDV 
Sbjct: 121 NQLTKDVAIKMYKDMITLQIMDNIFYEAQRQGRISFYLTSSGEEAIAIASAAALLPDDVV 180

Query: 181 LAQYREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIP 240
           LAQYREPGVL+WRGFTLQEFANQLFGNK DYGKGRQMPIHYGS  HNYFTISSPLGTQ+P
Sbjct: 181 LAQYREPGVLLWRGFTLQEFANQLFGNKGDYGKGRQMPIHYGSNHHNYFTISSPLGTQLP 240

Query: 241 QAVGIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTS 300
           QAVGIA+SLKMDK++AC VAYFGDGTTSEGDFHA LN AAVL APVIFICRNNGWAISTS
Sbjct: 241 QAVGIAYSLKMDKKEACVVAYFGDGTTSEGDFHAALNFAAVLRAPVIFICRNNGWAISTS 300

Query: 301 VQEQFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVG 360
           ++EQFRSDGVVVKGQGYGIRSIRIDGND LA+YSAVRKARSMA+ E MPVLIEALTYRVG
Sbjct: 301 IEEQFRSDGVVVKGQGYGIRSIRIDGNDTLAVYSAVRKARSMAIRENMPVLIEALTYRVG 360

Query: 361 HHSTSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------ 420
           HHSTSDDSSKYR  DEI++WKMERNP++RFAKW++NNGW S EDE  HKTT+K+      
Sbjct: 361 HHSTSDDSSKYRTTDEIQYWKMERNPMDRFAKWISNNGWFSQEDELNHKTTVKRQLLEAI 420

Query: 421 QNAEKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           QNAEKTEKPPLSELFNDVYD+LP NLQEQEEVLRQT KRYPQDYPSDVPL
Sbjct: 421 QNAEKTEKPPLSELFNDVYDNLPKNLQEQEEVLRQTRKRYPQDYPSDVPL 465

BLAST of CmaCh09G010730 vs. ExPASy TrEMBL
Match: A0A5A7V005 (2-oxoisovalerate dehydrogenase subunit alpha 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4863G00110 PE=4 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.9e-206
Identity = 357/425 (84.00%), Postives = 382/425 (89.88%), Query Frame = 0

Query: 43  TGSLWSTPRFESTEAVRRVDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCY 102
           +G LW T RFESTEA R VDQLYV ++DDQELDFPGG VPF +EMRFI EST +KRVPCY
Sbjct: 14  SGFLWPTCRFESTEAARHVDQLYVPNHDDQELDFPGGSVPFRNEMRFISEST-EKRVPCY 73

Query: 103 RVIDEIGEITTSNNFTQLRKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEA 162
           RV+ E G+I T NNF QL KD AIKMYKDMITLQ MD IFYEAQRQGRISFYLTSSGEEA
Sbjct: 74  RVLHETGDIITPNNFNQLTKDVAIKMYKDMITLQIMDNIFYEAQRQGRISFYLTSSGEEA 133

Query: 163 ISVASAAALHPDDVALAQYREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQ 222
           I+VASAAAL PDDV LAQYREPGVL+WRGFTLQEFANQLFGNK DYGKGRQMPIHYGS  
Sbjct: 134 IAVASAAALLPDDVVLAQYREPGVLLWRGFTLQEFANQLFGNKRDYGKGRQMPIHYGSNH 193

Query: 223 HNYFTISSPLGTQIPQAVGIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAP 282
           HNYFTISSPLGTQ+PQAVGIA+SLKMDK+DACAVAYFGDGTTSEGDFHA LN AAVLGAP
Sbjct: 194 HNYFTISSPLGTQLPQAVGIAYSLKMDKKDACAVAYFGDGTTSEGDFHAALNFAAVLGAP 253

Query: 283 VIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVA 342
           VIFICRNNGWAISTS++EQFRSDGVVVKGQGYGIRSIRIDGNDALA+Y+AV KARSMA+ 
Sbjct: 254 VIFICRNNGWAISTSIEEQFRSDGVVVKGQGYGIRSIRIDGNDALAVYTAVHKARSMAIR 313

Query: 343 EKMPVLIEALTYRVGHHSTSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDE 402
           EKMP+LIEALTYRVGHHSTSDDSSKYR  DEI++WKMERNPV+RFAKW++NNGW S EDE
Sbjct: 314 EKMPILIEALTYRVGHHSTSDDSSKYRTTDEIQYWKMERNPVDRFAKWISNNGWFSQEDE 373

Query: 403 YKHKTTIKK------QNAEKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYP 462
             HK T+KK      QNAEKTEKPPLSELFNDVYDHLP NLQEQEEVLRQT KR+PQDYP
Sbjct: 374 SNHKITVKKLLLEAIQNAEKTEKPPLSELFNDVYDHLPKNLQEQEEVLRQTRKRHPQDYP 433

BLAST of CmaCh09G010730 vs. NCBI nr
Match: XP_022974994.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 929.9 bits (2402), Expect = 8.7e-267
Identity = 461/467 (98.72%), Postives = 461/467 (98.72%), Query Frame = 0

Query: 1   MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR 60
           MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR
Sbjct: 1   MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR 60

Query: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL 120
           VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL
Sbjct: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL 120

Query: 121 RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ 180
           RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ
Sbjct: 121 RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ 180

Query: 181 YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV 240
           YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV
Sbjct: 181 YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV 240

Query: 241 GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE 300
           GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE
Sbjct: 241 GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE 300

Query: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS 360
           QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS
Sbjct: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS 360

Query: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QNA 420
           TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK      QNA
Sbjct: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKKQLLQAIQNA 420

Query: 421 EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL
Sbjct: 421 EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 467

BLAST of CmaCh09G010730 vs. NCBI nr
Match: KAG6592162.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 874.8 bits (2259), Expect = 3.3e-250
Identity = 433/467 (92.72%), Postives = 443/467 (94.86%), Query Frame = 0

Query: 1   MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR 60
           MIPFVRSSSRNLLALLNQN+ YPSLLCSVSE P+LVR   RWTGSLW TPRFESTEAVR 
Sbjct: 1   MIPFVRSSSRNLLALLNQNHRYPSLLCSVSEFPVLVRCGARWTGSLWFTPRFESTEAVRD 60

Query: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL 120
           VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGE T QKR PCYRVIDEIGE TTSNNFTQL
Sbjct: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGELTTQKRAPCYRVIDEIGETTTSNNFTQL 120

Query: 121 RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ 180
            KD AIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDV LAQ
Sbjct: 121 SKDAAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVVLAQ 180

Query: 181 YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV 240
           YREPGVL+WRGFTLQEFANQLFGNK DYGKGRQMPIHYGS QHNYFTISSPLGTQIPQAV
Sbjct: 181 YREPGVLLWRGFTLQEFANQLFGNKSDYGKGRQMPIHYGSNQHNYFTISSPLGTQIPQAV 240

Query: 241 GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE 300
           GIA+SLKMDK+DACAVAYFGDG+TSEGDFHAGLN AAVLGAPVIFICRNNGWAISTSVQE
Sbjct: 241 GIAYSLKMDKKDACAVAYFGDGSTSEGDFHAGLNFAAVLGAPVIFICRNNGWAISTSVQE 300

Query: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS 360
           QFRSDGVVVKGQGYGIRSIRIDGNDALA+YSAVR+ARSMAVAE+MPVLIEALTYRVGHHS
Sbjct: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAVYSAVRRARSMAVAEQMPVLIEALTYRVGHHS 360

Query: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QNA 420
           TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDE KHKTTIKK      QNA
Sbjct: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDESKHKTTIKKQLLQAIQNA 420

Query: 421 EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           EKTEKPPLSELF+DVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL
Sbjct: 421 EKTEKPPLSELFSDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 467

BLAST of CmaCh09G010730 vs. NCBI nr
Match: XP_022936690.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 873.6 bits (2256), Expect = 7.4e-250
Identity = 434/467 (92.93%), Postives = 444/467 (95.07%), Query Frame = 0

Query: 1   MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR 60
           MIPFVRSSSRNLLALLNQN+ Y SLLCSVSESP+LVR   RWTGSLW TPRFESTEAVR 
Sbjct: 1   MIPFVRSSSRNLLALLNQNHRYLSLLCSVSESPVLVRCGARWTGSLWFTPRFESTEAVRD 60

Query: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL 120
           VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGES  QKRVPCYRVIDEIGE TTSNNFTQL
Sbjct: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESATQKRVPCYRVIDEIGETTTSNNFTQL 120

Query: 121 RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ 180
            KD AIKMYKDMITLQT+DTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDV LAQ
Sbjct: 121 SKDGAIKMYKDMITLQTVDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVVLAQ 180

Query: 181 YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV 240
           YREPGVL+WRGFTLQEFANQLFGNK DYGKGRQMPIHYGS QHNYFTISSPLGTQIPQAV
Sbjct: 181 YREPGVLLWRGFTLQEFANQLFGNKSDYGKGRQMPIHYGSIQHNYFTISSPLGTQIPQAV 240

Query: 241 GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE 300
           GIA+SLKMDK+DACAVAYFGDGTTSEGDFHAGLN AAVLGAPVIFICRNNGWAISTSVQE
Sbjct: 241 GIAYSLKMDKKDACAVAYFGDGTTSEGDFHAGLNFAAVLGAPVIFICRNNGWAISTSVQE 300

Query: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS 360
           QFRSDGVVVKGQGYGIRSIRIDGNDALA+YSAVR+ARSMAVAE+MPVLIEALTYRVGHHS
Sbjct: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAVYSAVRRARSMAVAEQMPVLIEALTYRVGHHS 360

Query: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QNA 420
           TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDE KHKTTIKK      QNA
Sbjct: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDESKHKTTIKKQLLQAIQNA 420

Query: 421 EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           EKTEKPPLSELF+DVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL
Sbjct: 421 EKTEKPPLSELFSDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 467

BLAST of CmaCh09G010730 vs. NCBI nr
Match: XP_023536387.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 859.4 bits (2219), Expect = 1.4e-245
Identity = 429/467 (91.86%), Postives = 438/467 (93.79%), Query Frame = 0

Query: 1   MIPFVRSSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRR 60
           MIPFVRSSSRNLLALLNQN+LYPSLL SVSESP+       W GSLWST RFESTEAVR 
Sbjct: 1   MIPFVRSSSRNLLALLNQNHLYPSLLSSVSESPV-------WIGSLWSTTRFESTEAVRH 60

Query: 61  VDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQL 120
           VDQLYVHDNDDQELDFPGGKVPFTSE+RFIGEST QKRVPCYRVIDEIGE  TSN FTQL
Sbjct: 61  VDQLYVHDNDDQELDFPGGKVPFTSELRFIGESTTQKRVPCYRVIDEIGETATSNKFTQL 120

Query: 121 RKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQ 180
            KD  IKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDV LAQ
Sbjct: 121 SKDAGIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVVLAQ 180

Query: 181 YREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAV 240
           YREPGVL+WRGFTLQEFANQLFGNK DYGKGRQMPIHYGS QHNYFTISSPLGTQIPQAV
Sbjct: 181 YREPGVLLWRGFTLQEFANQLFGNKSDYGKGRQMPIHYGSNQHNYFTISSPLGTQIPQAV 240

Query: 241 GIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQE 300
           GIA+SLKMDK+DACAVAYFGDGTTSEGDFHAGLN AAVLGAPVIFICRNNGWAISTSVQE
Sbjct: 241 GIAYSLKMDKKDACAVAYFGDGTTSEGDFHAGLNFAAVLGAPVIFICRNNGWAISTSVQE 300

Query: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHS 360
           QFRSDGVVVKGQGYGIRSIRIDGNDALA+YSAVRKARSMAVAE+MPVLIEALTYRVGHHS
Sbjct: 301 QFRSDGVVVKGQGYGIRSIRIDGNDALAVYSAVRKARSMAVAEQMPVLIEALTYRVGHHS 360

Query: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QNA 420
           TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDE KHKTTIKK      Q+A
Sbjct: 361 TSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDESKHKTTIKKQLLQAIQSA 420

Query: 421 EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL
Sbjct: 421 EKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 460

BLAST of CmaCh09G010730 vs. NCBI nr
Match: XP_008445779.1 (PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial [Cucumis melo])

HSP 1 Score: 740.7 bits (1911), Expect = 7.5e-210
Identity = 374/472 (79.24%), Postives = 406/472 (86.02%), Query Frame = 0

Query: 1   MIPFVRSSSRNLL-ALLNQNYLYPSLLCSVSESPILVRYDGR----WTGSLWSTPRFEST 60
           MI FVRSSS NL+  +LN+N+         S+S + +R   R     +G LW T RFEST
Sbjct: 1   MIRFVRSSSPNLIHRILNRNF-------HCSDSSMALRCGRRRKDYSSGFLWPTCRFEST 60

Query: 61  EAVRRVDQLYVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSN 120
           EA R VDQLYV ++DDQELDFPGG VPF +EMRFI EST +KRVPCYRV+ E G+I T N
Sbjct: 61  EAARHVDQLYVPNHDDQELDFPGGSVPFRNEMRFISEST-EKRVPCYRVLHETGDIITPN 120

Query: 121 NFTQLRKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDD 180
           NF QL KD AIKMYKDMITLQ MD IFYEAQRQGRISFYLTSSGEEAI+VASAAAL PDD
Sbjct: 121 NFNQLTKDVAIKMYKDMITLQIMDNIFYEAQRQGRISFYLTSSGEEAIAVASAAALLPDD 180

Query: 181 VALAQYREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQ 240
           V LAQYREPGVL+WRGFTLQEFANQLFGNK DYGKGRQMPIHYGS  HNYFTISSPLGTQ
Sbjct: 181 VVLAQYREPGVLLWRGFTLQEFANQLFGNKRDYGKGRQMPIHYGSNHHNYFTISSPLGTQ 240

Query: 241 IPQAVGIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAIS 300
           +PQAVGIA+SLKMDK+DACAVAYFGDGTTSEGDFHA LN AAVLGAPVIFICRNNGWAIS
Sbjct: 241 LPQAVGIAYSLKMDKKDACAVAYFGDGTTSEGDFHAALNFAAVLGAPVIFICRNNGWAIS 300

Query: 301 TSVQEQFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYR 360
           TS++EQFRSDGVVVKGQGYGIRSIRIDGNDALA+Y+AV KARSMA+ EKMP+LIEALTYR
Sbjct: 301 TSIEEQFRSDGVVVKGQGYGIRSIRIDGNDALAVYTAVHKARSMAIREKMPILIEALTYR 360

Query: 361 VGHHSTSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK---- 420
           VGHHSTSDDSSKYR  DEI++WKMERNPV+RFAKW++NNGW S EDE  HK T+KK    
Sbjct: 361 VGHHSTSDDSSKYRTTDEIQYWKMERNPVDRFAKWISNNGWFSQEDESNHKITVKKLLLE 420

Query: 421 --QNAEKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
             QNAEKTEKPPLSELFNDVYDHLP NLQEQEEVLRQT KR+PQDYPSDVPL
Sbjct: 421 AIQNAEKTEKPPLSELFNDVYDHLPKNLQEQEEVLRQTRKRHPQDYPSDVPL 464

BLAST of CmaCh09G010730 vs. TAIR 10
Match: AT1G21400.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 574.3 bits (1479), Expect = 8.7e-164
Identity = 282/414 (68.12%), Postives = 331/414 (79.95%), Query Frame = 0

Query: 51  RFESTEAVRRVDQL--YVHDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEI 110
           R EST    + D L   + + D QELDFPGGKV +TSEM+FI ES+  +R+PCYRV+DE 
Sbjct: 55  RHESTAVETQADHLVQQIDEVDAQELDFPGGKVGYTSEMKFIPESS-SRRIPCYRVLDED 114

Query: 111 GEITTSNNFTQLRKDEAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASA 170
           G I   ++F  + +  A++MY+ M TLQ MD IFYEAQRQGRISFYLTS GEEAI++ASA
Sbjct: 115 GRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASA 174

Query: 171 AALHPDDVALAQYREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTI 230
           AAL PDDV L QYREPGVL+WRGFTL+EFANQ FGNK DYGKGRQMPIHYGS + NYFTI
Sbjct: 175 AALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTI 234

Query: 231 SSPLGTQIPQAVGIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICR 290
           SSP+ TQ+PQA G+ +SLKMDK++AC V + GDG TSEGDFHAGLN AAV+ APV+FICR
Sbjct: 235 SSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICR 294

Query: 291 NNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVL 350
           NNGWAIST + EQFRSDG+VVKGQ YGIRSIR+DGNDALA+YSAVR AR MAV E+ PVL
Sbjct: 295 NNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVL 354

Query: 351 IEALTYRVGHHSTSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTT 410
           IE +TYRVGHHSTSDDS+KYRA DEI++WKM RNPVNRF KW+ +NGW S EDE K ++ 
Sbjct: 355 IEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSN 414

Query: 411 IKK------QNAEKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYP 457
            +K      Q AEK EK PL+ELFNDVYD  P NL+EQE  L++  K+ PQDYP
Sbjct: 415 ARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYP 467

BLAST of CmaCh09G010730 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 554.3 bits (1427), Expect = 9.3e-158
Identity = 279/464 (60.13%), Postives = 345/464 (74.35%), Query Frame = 0

Query: 7   SSSRNLLALLNQNYLYPSLLCSVSESPILVRYDGRWTGSLWSTPRFESTEAVRRVDQLYV 66
           SS   LL +L  N  + S          ++ +D    GS     R  +T A       +V
Sbjct: 10  SSKSTLLNILRHNLGFGSRSHVTRHIRQILPHDPPLRGSQNPISRLCNTMAEPETLSSFV 69

Query: 67  ---HDNDDQELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQLRKD 126
              + N+ Q +DFPGGKV FT E++FI ES  ++RVPCYRV+D+ G++ T++ F Q+ ++
Sbjct: 70  QHEYANNHQVMDFPGGKVAFTPEIQFISESD-KERVPCYRVLDDNGQLITNSQFVQVSEE 129

Query: 127 EAIKMYKDMITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQYRE 186
            A+K+Y DM+TLQ MD IFYEAQRQGR+SFY T+ GEEAI++ASAAAL P DV   QYRE
Sbjct: 130 VAVKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYRE 189

Query: 187 PGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAVGIA 246
           PGVL+WRGFTLQEFANQ FGNK DYGKGRQMP+HYGS + NYFT+S+ + TQ+P AVG A
Sbjct: 190 PGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAA 249

Query: 247 HSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQEQFR 306
           +SLKMDK+DACAV YFGDG TSEGDFHA LN+AAV+ APV+FICRNNGWAIST   +QFR
Sbjct: 250 YSLKMDKKDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFR 309

Query: 307 SDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHSTSD 366
           SDGVVVKG+ YGIRSIR+DGNDALA+YSAV  AR MA+ E+ P+LIEALTYRVGHHSTSD
Sbjct: 310 SDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSD 369

Query: 367 DSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKKQN------AEKT 426
           DS++YR+  EIE W   RNP++RF  W+ +NGW S + E   ++ IKK+       AEKT
Sbjct: 370 DSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKT 429

Query: 427 EKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           EKP L  +F+DVYD  PSNL+EQE ++RQT   +PQDYPSDVPL
Sbjct: 430 EKPNLQNMFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

BLAST of CmaCh09G010730 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 549.7 bits (1415), Expect = 2.3e-156
Identity = 264/396 (66.67%), Postives = 321/396 (81.06%), Query Frame = 0

Query: 72  QELDFPGGKVPFTSEMRFIGESTIQKRVPCYRVIDEIGEITTSNNFTQLRKDEAIKMYKD 131
           Q +DFPGGKV FT E++FI ES  ++RVPCYRV+D+ G++ T++ F Q+ ++ A+K+Y D
Sbjct: 7   QVMDFPGGKVAFTPEIQFISESD-KERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSD 66

Query: 132 MITLQTMDTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALAQYREPGVLVWRG 191
           M+TLQ MD IFYEAQRQGR+SFY T+ GEEAI++ASAAAL P DV   QYREPGVL+WRG
Sbjct: 67  MVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRG 126

Query: 192 FTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQAVGIAHSLKMDKQ 251
           FTLQEFANQ FGNK DYGKGRQMP+HYGS + NYFT+S+ + TQ+P AVG A+SLKMDK+
Sbjct: 127 FTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKK 186

Query: 252 DACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKG 311
           DACAV YFGDG TSEGDFHA LN+AAV+ APV+FICRNNGWAIST   +QFRSDGVVVKG
Sbjct: 187 DACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKG 246

Query: 312 QGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHHSTSDDSSKYRAV 371
           + YGIRSIR+DGNDALA+YSAV  AR MA+ E+ P+LIEALTYRVGHHSTSDDS++YR+ 
Sbjct: 247 RAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSA 306

Query: 372 DEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKKQN------AEKTEKPPLSEL 431
            EIE W   RNP++RF  W+ +NGW S + E   ++ IKK+       AEKTEKP L  +
Sbjct: 307 GEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQNM 366

Query: 432 FNDVYDHLPSNLQEQEEVLRQTTKRYPQDYPSDVPL 462
           F+DVYD  PSNL+EQE ++RQT   +PQDYPSDVPL
Sbjct: 367 FSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 401

BLAST of CmaCh09G010730 vs. TAIR 10
Match: AT5G34780.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 7.3e-78
Identity = 142/222 (63.96%), Postives = 173/222 (77.93%), Query Frame = 0

Query: 240 VGIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQ 299
           +G++ +    +++ACAV + GDG TSEGDFHAGLN AAV+ APV+FICRNNGWAIST + 
Sbjct: 15  IGLSKAKDCWEKNACAVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHIS 74

Query: 300 EQFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHH 359
           EQFRSDG+VVKGQ YGIRSIR+DGNDALA+YSAV  AR MAV E+ PVLIE + YRVGHH
Sbjct: 75  EQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVCSAREMAVTEQRPVLIEMMIYRVGHH 134

Query: 360 STSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKHKTTIKK------QN 419
           STSDDS+KYRA DEI++WKM RN VNRF K + +NGW S EDE K ++  +K      Q 
Sbjct: 135 STSDDSTKYRAADEIQYWKMSRNSVNRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQA 194

Query: 420 AEKTEKPPLSELFNDVYDHLPSNLQEQEEVLRQTTKRYPQDY 456
           AEK EK PL+ELFNDVYD  P NL+E+E  L++  ++ PQDY
Sbjct: 195 AEKWEKQPLTELFNDVYDVKPKNLEEEELGLKELIEKQPQDY 236

BLAST of CmaCh09G010730 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 147.5 bits (371), Expect = 2.6e-35
Identity = 93/318 (29.25%), Postives = 163/318 (51.26%), Query Frame = 0

Query: 124 EAIKMYKDMITLQTM----DTIFYEAQRQGRISFYLTSSGEEAISVASAAALHPDDVALA 183
           E +  ++ M  ++ M    D+++     +G    Y    G+EA+++   AA+   D  + 
Sbjct: 58  ELLDFFRTMALMRRMEIAADSLYKAKLIRGFCHLY---DGQEAVAIGMEAAITKKDAIIT 117

Query: 184 QYREPGVLVWRGFTLQEFANQLFGNKDDYGKGRQMPIHYGSKQHNYFTISSPLGTQIPQA 243
            YR+  + + RG +L E  ++L G +    KG+   +H+  K+ +++     +G Q+P  
Sbjct: 118 AYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPLG 177

Query: 244 VGIAHSLKMDKQDACAVAYFGDGTTSEGDFHAGLNLAAVLGAPVIFICRNNGWAISTSVQ 303
            GIA + K +K++A   A +GDG  ++G     LN++A+   P I +C NN + + T+  
Sbjct: 178 CGIAFAQKYNKEEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTAEW 237

Query: 304 EQFRSDGVVVKGQGYGIRSIRIDGNDALAIYSAVRKARSMAVAEKMPVLIEALTYRVGHH 363
              +S     +G    +  +++DG DA A+  A + A+  A+ EK P+++E  TYR   H
Sbjct: 238 RAAKSPSYYKRGD--YVPGLKVDGMDAFAVKQACKFAKQHAL-EKGPIILEMDTYRYHGH 297

Query: 364 STSDDSSKYRAVDEIEHWKMERNPVNRFAKWLTNNGWLSVEDEYKH-KTTIKKQ------ 423
           S SD  S YR  DEI   + ER+P+ R  K + ++  L+ E E K  +  I+K+      
Sbjct: 298 SMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHD-LATEKELKDMEKEIRKEVDDAIA 357

Query: 424 NAEKTEKPPLSELFNDVY 431
            A+    P  SELF +VY
Sbjct: 358 KAKDCPMPEPSELFTNVY 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LPL51.2e-16268.122-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis tha... [more]
Q84JL21.3e-15660.132-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis tha... [more]
P501367.8e-10948.732-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=1... [more]
P119601.3e-10848.982-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (Fragment) OS=Rattus... [more]
A5A6H93.9e-10848.982-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes O... [more]
Match NameE-valueIdentityDescription
A0A6J1IJ534.2e-26798.722-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like OS=Cucurbita ... [more]
A0A6J1FEF03.6e-25092.932-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like OS=Cucurbita ... [more]
A0A1S3BD093.6e-21079.242-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Cucumis melo OX... [more]
A0A0A0KIX51.7e-20778.09E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G395130 PE=4 ... [more]
A0A5A7V0051.9e-20684.002-oxoisovalerate dehydrogenase subunit alpha 1 OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
XP_022974994.18.7e-26798.722-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like [Cucurbita ma... [more]
KAG6592162.13.3e-25092.722-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial, partial [Cucurbit... [more]
XP_022936690.17.4e-25092.932-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like [Cucurbita mo... [more]
XP_023536387.11.4e-24591.862-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like [Cucurbita pe... [more]
XP_008445779.17.5e-21079.24PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial [Cucumi... [more]
Match NameE-valueIdentityDescription
AT1G21400.18.7e-16468.12Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.19.3e-15860.13Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.22.3e-15666.67Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G34780.17.3e-7863.96Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT1G59900.12.6e-3529.25pyruvate dehydrogenase complex E1 alpha subunit [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.970coord: 69..459
e-value: 1.7E-140
score: 470.4
NoneNo IPR availablePANTHERPTHR43380:SF112-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIALcoord: 49..461
NoneNo IPR availablePANTHERPTHR433802-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIALcoord: 49..461
NoneNo IPR availableCDDcd02000TPP_E1_PDC_ADC_BCADCcoord: 128..412
e-value: 2.02963E-142
score: 407.652
IPR001017Dehydrogenase, E1 componentPFAMPF00676E1_dhcoord: 130..418
e-value: 7.0E-89
score: 297.7
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 99..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh09G010730.1CmaCh09G010730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009083 branched-chain amino acid catabolic process
cellular_component GO:0005947 mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor