CmaCh09G009490 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh09G009490
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptiontRNA-uridine aminocarboxypropyltransferase
LocationCma_Chr09: 4767100 .. 4768188 (+)
RNA-Seq ExpressionCmaCh09G009490
SyntenyCmaCh09G009490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTCATTTTCGATTTCGGATCCGGTTCGGAAACTCTAAAAACCCGCTAAATCGCATTCCTCCAAATCATCTTCAATTCAAGCTTCTTCAAACCCTAGCTATGGCGGATGTTCCTCCCAGTTCCGGAAGACTCGTTTGTCCATCCTGTTCGAAACCGGCTCGTATCTGTCTCTGTTCGCGGCTCCAAAGCCCTAGTCTCGAGAACTCAGTCGGAGTAATCATTCTTCAACACAGTTTAGAGAAAAAGCACCCGCTAAATTCTTCTAGAATCGCAAAATTAGGTCTCAAGAATGTCGAGATCGCTACGGTTTCCGACGTTAATTTCGAGGCTCGATTCACTATTCGGTTGCCTGAACTAAATTCTGATGAAGAATTTAGTTTCGGAAATGGTCAAAATGCTGCTCAAAAGCCTCAAATTCAAGTACAGAATCGGGATGAATCTCTAGTTGATAAACCTACTTGCACAAAATCTGACGAAGGAGCTGCGATTACCGCTACGATTGGGAAATATGGCGTCGTTAACTCATTCGATCACATCTGGATGCATCAACCGAGTTTGCAGGATCTTAAAATCGATGAGACTTTCGCATCTCCAGAAATTAGGGCATCAATGGCGAAAGGATTCATCGTGAAGAAACTGCAGAAGCGGAAGGTGGATGAAAGCAAGGAGCTAGAGGAATATGCCGAGTTCGAAATTGAGGTTCCTCCCAAGTCAGTACTACTTTTTCCCTCTGATAATGCTTTTACTGTAAATGATGATGATGTTCATGGTAGTGATTTTCATGTCAATAATTTGATAGTTTTAGATGGAACATGGGCAAAAGCTAAGAGAATGTACAATGAAAATCCTTGGTTGAGGCTTTTGCCTCATATGAAGTTGGATTTAGAGAAGATGAGCTTGTATAGTGAAGTGAGACGTCAGCCTAAGACTGGTTTTTTGTCCACCATTGAAAGCATTGTCTATGCCTTGAAAGTAATAGGTGATCACCCTGAGGGGCTGGATGATTTGTTGGATGTTTTTGAATCCATGATAGGAGATCAAAGGAGATGTAAAGATGAGAGGTTGAGAATTACTACTCAAATTTAA

mRNA sequence

ATGCTTCATTTTCGATTTCGGATCCGGTTCGGAAACTCTAAAAACCCGCTAAATCGCATTCCTCCAAATCATCTTCAATTCAAGCTTCTTCAAACCCTAGCTATGGCGGATGTTCCTCCCAGTTCCGGAAGACTCGTTTGTCCATCCTGTTCGAAACCGGCTCGTATCTGTCTCTGTTCGCGGCTCCAAAGCCCTAGTCTCGAGAACTCAGTCGGAGTAATCATTCTTCAACACAGTTTAGAGAAAAAGCACCCGCTAAATTCTTCTAGAATCGCAAAATTAGGTCTCAAGAATGTCGAGATCGCTACGGTTTCCGACGTTAATTTCGAGGCTCGATTCACTATTCGGTTGCCTGAACTAAATTCTGATGAAGAATTTAGTTTCGGAAATGGTCAAAATGCTGCTCAAAAGCCTCAAATTCAAGTACAGAATCGGGATGAATCTCTAGTTGATAAACCTACTTGCACAAAATCTGACGAAGGAGCTGCGATTACCGCTACGATTGGGAAATATGGCGTCGTTAACTCATTCGATCACATCTGGATGCATCAACCGAGTTTGCAGGATCTTAAAATCGATGAGACTTTCGCATCTCCAGAAATTAGGGCATCAATGGCGAAAGGATTCATCGTGAAGAAACTGCAGAAGCGGAAGGTGGATGAAAGCAAGGAGCTAGAGGAATATGCCGAGTTCGAAATTGAGGTTCCTCCCAAGTCAGTACTACTTTTTCCCTCTGATAATGCTTTTACTGTAAATGATGATGATGTTCATGGTAGTGATTTTCATGTCAATAATTTGATAGTTTTAGATGGAACATGGGCAAAAGCTAAGAGAATGTACAATGAAAATCCTTGGTTGAGGCTTTTGCCTCATATGAAGTTGGATTTAGAGAAGATGAGCTTGTATAGTGAAGTGAGACGTCAGCCTAAGACTGGTTTTTTGTCCACCATTGAAAGCATTGTCTATGCCTTGAAAGTAATAGGTGATCACCCTGAGGGGCTGGATGATTTGTTGGATGTTTTTGAATCCATGATAGGAGATCAAAGGAGATGTAAAGATGAGAGGTTGAGAATTACTACTCAAATTTAA

Coding sequence (CDS)

ATGCTTCATTTTCGATTTCGGATCCGGTTCGGAAACTCTAAAAACCCGCTAAATCGCATTCCTCCAAATCATCTTCAATTCAAGCTTCTTCAAACCCTAGCTATGGCGGATGTTCCTCCCAGTTCCGGAAGACTCGTTTGTCCATCCTGTTCGAAACCGGCTCGTATCTGTCTCTGTTCGCGGCTCCAAAGCCCTAGTCTCGAGAACTCAGTCGGAGTAATCATTCTTCAACACAGTTTAGAGAAAAAGCACCCGCTAAATTCTTCTAGAATCGCAAAATTAGGTCTCAAGAATGTCGAGATCGCTACGGTTTCCGACGTTAATTTCGAGGCTCGATTCACTATTCGGTTGCCTGAACTAAATTCTGATGAAGAATTTAGTTTCGGAAATGGTCAAAATGCTGCTCAAAAGCCTCAAATTCAAGTACAGAATCGGGATGAATCTCTAGTTGATAAACCTACTTGCACAAAATCTGACGAAGGAGCTGCGATTACCGCTACGATTGGGAAATATGGCGTCGTTAACTCATTCGATCACATCTGGATGCATCAACCGAGTTTGCAGGATCTTAAAATCGATGAGACTTTCGCATCTCCAGAAATTAGGGCATCAATGGCGAAAGGATTCATCGTGAAGAAACTGCAGAAGCGGAAGGTGGATGAAAGCAAGGAGCTAGAGGAATATGCCGAGTTCGAAATTGAGGTTCCTCCCAAGTCAGTACTACTTTTTCCCTCTGATAATGCTTTTACTGTAAATGATGATGATGTTCATGGTAGTGATTTTCATGTCAATAATTTGATAGTTTTAGATGGAACATGGGCAAAAGCTAAGAGAATGTACAATGAAAATCCTTGGTTGAGGCTTTTGCCTCATATGAAGTTGGATTTAGAGAAGATGAGCTTGTATAGTGAAGTGAGACGTCAGCCTAAGACTGGTTTTTTGTCCACCATTGAAAGCATTGTCTATGCCTTGAAAGTAATAGGTGATCACCCTGAGGGGCTGGATGATTTGTTGGATGTTTTTGAATCCATGATAGGAGATCAAAGGAGATGTAAAGATGAGAGGTTGAGAATTACTACTCAAATTTAA

Protein sequence

MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCSRLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPELNSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHIWMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITTQI
Homology
BLAST of CmaCh09G009490 vs. ExPASy Swiss-Prot
Match: Q47319 (tRNA-uridine aminocarboxypropyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=tapT PE=1 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 3.1e-04
Identity = 23/63 (36.51%), Postives = 38/63 (60.32%), Query Frame = 0

Query: 267 IVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMSLYSEVRRQPKTGFLSTIESIVYALKV 326
           I+LDGTW +A++M+ ++P+L  LP + +DL ++S Y  +R     G   T E  +  L +
Sbjct: 134 IMLDGTWPEARKMFRKSPYLDNLPVISVDLSRLSAY-RLREAQAEGQYCTAEVAIALLDM 193

Query: 327 IGD 330
            GD
Sbjct: 194 AGD 195

BLAST of CmaCh09G009490 vs. ExPASy Swiss-Prot
Match: Q9D0U1 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Mus musculus OX=10090 GN=Dtwd2 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 5.3e-04
Identity = 27/89 (30.34%), Postives = 50/89 (56.18%), Query Frame = 0

Query: 239 SVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEK 298
           +++L+P   A T  ++ +  S  + + +I++DGTW++AK ++ +N   RL   ++L    
Sbjct: 148 TLILYPGAEA-TNLEEFILDSPVYPSTIILIDGTWSQAKDIFYKNSLFRLPKQVQLKTSV 207

Query: 299 MSLYSEVRRQPKTGFLSTIESIVYALKVI 328
            S Y  +R QP    LST+E    AL ++
Sbjct: 208 CSQY-VIRMQPTNRCLSTLECAAVALSIL 234

BLAST of CmaCh09G009490 vs. ExPASy TrEMBL
Match: A0A6J1IMF8 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476900 PE=4 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 9.5e-206
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 0

Query: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60
           MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60

Query: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120
           RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL
Sbjct: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120

Query: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180
           NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI
Sbjct: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180

Query: 181 WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV 240
           WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV
Sbjct: 181 WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV 240

Query: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300
           LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS
Sbjct: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300

Query: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360
           LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT
Sbjct: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360

Query: 361 QI 363
           QI
Sbjct: 361 QI 362

BLAST of CmaCh09G009490 vs. ExPASy TrEMBL
Match: A0A6J1FCX1 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111442857 PE=4 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.7e-199
Identity = 351/362 (96.96%), Postives = 355/362 (98.07%), Query Frame = 0

Query: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60
           MLHFRFRIRFGNSKNPLNR PPNHLQFKLLQTLAMADVPPS+GRLVCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRFPPNHLQFKLLQTLAMADVPPSTGRLVCPSCSKPARICLCS 60

Query: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120
           RL +PSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIA VSDVNFEARFTIRLPEL
Sbjct: 61  RLHNPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIAPVSDVNFEARFTIRLPEL 120

Query: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180
           NSDEEFSFGNGQNAAQKPQIQVQNRDESLV KPTCTKSDEGAAITATIGKYGVVNSF HI
Sbjct: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVGKPTCTKSDEGAAITATIGKYGVVNSFKHI 180

Query: 181 WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV 240
           WMHQPSLQDLKIDE FASPEIR+SMAKGFIVKKLQKR+VDESKELEEYAEFEIEVPPKSV
Sbjct: 181 WMHQPSLQDLKIDEIFASPEIRSSMAKGFIVKKLQKRQVDESKELEEYAEFEIEVPPKSV 240

Query: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300
           LLFPSDNAFTVNDDDVH SDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS
Sbjct: 241 LLFPSDNAFTVNDDDVHSSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300

Query: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360
           LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT
Sbjct: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360

Query: 361 QI 363
           QI
Sbjct: 361 QI 362

BLAST of CmaCh09G009490 vs. ExPASy TrEMBL
Match: A0A1S3BZW7 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo OX=3656 GN=LOC103495005 PE=4 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.2e-154
Identity = 290/368 (78.80%), Postives = 315/368 (85.60%), Query Frame = 0

Query: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60
           M+HF FRIRFGN K+ LN + PN LQ KL   LAMADV PSS R VCPSCSKPARICLCS
Sbjct: 1   MIHFPFRIRFGNPKSLLNPL-PNRLQSKLFNNLAMADVLPSSRRPVCPSCSKPARICLCS 60

Query: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120
           R +SPSLENSVG+IILQHS EK HPLNS+RIAKLGLKNV+IATVSDVNFEA FTIRLPE 
Sbjct: 61  RFRSPSLENSVGIIILQHSSEKNHPLNSARIAKLGLKNVDIATVSDVNFEAHFTIRLPEP 120

Query: 121 NS-------DEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGV 180
           NS       D E SF NG NA QK QIQ    DES+VDK   TK+++GAAI+ATIGKYGV
Sbjct: 121 NSAAQNLDPDAECSFRNGHNATQKSQIQ----DESIVDKLNSTKANKGAAISATIGKYGV 180

Query: 181 VNSFDHIWMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEI 240
           VNSFDHIWM QP  Q+LKI+E  ASPEIRAS+AKGFIVKKLQKRK+ ESK LEEYAEFEI
Sbjct: 181 VNSFDHIWMQQPGFQELKINEVLASPEIRASLAKGFIVKKLQKRKLVESKGLEEYAEFEI 240

Query: 241 EVPPKSVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMK 300
           +VPPKSVLLFPS+NAFTV+   V GSDF++N+LIVLDGTWAKAKRMYNENPWLRLLPHMK
Sbjct: 241 QVPPKSVLLFPSENAFTVS-GGVDGSDFYINHLIVLDGTWAKAKRMYNENPWLRLLPHMK 300

Query: 301 LDLEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKD 360
           LDLE MSLYSEVR QPK GFLSTIESIVYALK+IGDHPEGLDDLLDVFESMIGDQR+CKD
Sbjct: 301 LDLEMMSLYSEVRHQPKIGFLSTIESIVYALKLIGDHPEGLDDLLDVFESMIGDQRKCKD 360

Query: 361 ERLRITTQ 362
           ERL+IT Q
Sbjct: 361 ERLKITCQ 362

BLAST of CmaCh09G009490 vs. ExPASy TrEMBL
Match: A0A0A0LD09 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G851850 PE=4 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 6.7e-151
Identity = 286/368 (77.72%), Postives = 309/368 (83.97%), Query Frame = 0

Query: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60
           M+HF F IRFGN KN LN  P  H Q KL   L MADV PSS R VCPSCSKPARICLCS
Sbjct: 12  MIHFSFPIRFGNPKNLLNPQPNRH-QSKLFHNLPMADVLPSSRRPVCPSCSKPARICLCS 71

Query: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120
           R +S S+ENSVGVIILQHSLEK HPLNS+RI KLGLKNVEIATVSDVNFEARFTIRLPE 
Sbjct: 72  RFRSSSVENSVGVIILQHSLEKNHPLNSARIVKLGLKNVEIATVSDVNFEARFTIRLPEP 131

Query: 121 NS-------DEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGV 180
           NS       D E SF NG    QKPQIQ    D S+VDK  CTK++EGAAI+ TIGK+GV
Sbjct: 132 NSAAQNLDPDIECSFRNGHGTIQKPQIQ----DGSIVDKLNCTKTNEGAAISVTIGKHGV 191

Query: 181 VNSFDHIWMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEI 240
           VNSFDHIWM  P  Q+LKI+E  ASPEIRAS+AKGFIVKK+QKR++ ESK LEEYAEFEI
Sbjct: 192 VNSFDHIWMQLPGFQELKINEFLASPEIRASLAKGFIVKKMQKRQLVESKGLEEYAEFEI 251

Query: 241 EVPPKSVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMK 300
           +VPPKSVLLFPS+NAFTV+   V GSDF++NNLIVLDGTWAKAKRMYNENPWLRLLPHMK
Sbjct: 252 QVPPKSVLLFPSENAFTVS-GGVDGSDFYINNLIVLDGTWAKAKRMYNENPWLRLLPHMK 311

Query: 301 LDLEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKD 360
           LDLE MSLYSEVR QPK GFLSTIESIVYALK+IGDHPEGLDDLLDVFESMIGDQRRCKD
Sbjct: 312 LDLEIMSLYSEVRHQPKIGFLSTIESIVYALKLIGDHPEGLDDLLDVFESMIGDQRRCKD 371

Query: 361 ERLRITTQ 362
           ERL+IT+Q
Sbjct: 372 ERLKITSQ 373

BLAST of CmaCh09G009490 vs. ExPASy TrEMBL
Match: A0A5A7T5E2 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001820 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 3.0e-143
Identity = 269/334 (80.54%), Postives = 291/334 (87.13%), Query Frame = 0

Query: 35  MADVPPSSGRLVCPSCSKPARICLCSRLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKL 94
           MADV PSS R VCPSCSKPARICLCSR +SPSLENSVG+IILQHS EK HPLNS+RIAKL
Sbjct: 1   MADVLPSSRRPVCPSCSKPARICLCSRFRSPSLENSVGIIILQHSSEKNHPLNSARIAKL 60

Query: 95  GLKNVEIATVSDVNFEARFTIRLPELNS-------DEEFSFGNGQNAAQKPQIQVQNRDE 154
           GLKNV+IATVSDVNFEA FTIRLPE NS       D E SF NG NA QK QIQ    DE
Sbjct: 61  GLKNVDIATVSDVNFEAHFTIRLPEPNSAAQNLDPDAECSFRNGHNATQKSQIQ----DE 120

Query: 155 SLVDKPTCTKSDEGAAITATIGKYGVVNSFDHIWMHQPSLQDLKIDETFASPEIRASMAK 214
           S+VDK   TK+++GAAI+ATIGKYGVVNSFDHIWM QP  Q+LKI+E  ASPEIRAS+AK
Sbjct: 121 SIVDKLNSTKANKGAAISATIGKYGVVNSFDHIWMQQPGFQELKINEVLASPEIRASLAK 180

Query: 215 GFIVKKLQKRKVDESKELEEYAEFEIEVPPKSVLLFPSDNAFTVNDDDVHGSDFHVNNLI 274
           GFIVKKLQKRK+ ESK LEEYAEFEI+VPPKSVLLFPS+NAFTV+   V GSDF++N+LI
Sbjct: 181 GFIVKKLQKRKLVESKGLEEYAEFEIQVPPKSVLLFPSENAFTVS-GGVDGSDFYINHLI 240

Query: 275 VLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMSLYSEVRRQPKTGFLSTIESIVYALKVI 334
           VLDGTWAKAKRMYNENPWLRLLPHMKLDLE MSLYSEVR QPK GFLSTIESIVYALK+I
Sbjct: 241 VLDGTWAKAKRMYNENPWLRLLPHMKLDLEMMSLYSEVRHQPKIGFLSTIESIVYALKLI 300

Query: 335 GDHPEGLDDLLDVFESMIGDQRRCKDERLRITTQ 362
           GDHPEGLDDLLDVFESMIGDQR+CKDERL+IT Q
Sbjct: 301 GDHPEGLDDLLDVFESMIGDQRKCKDERLKITCQ 329

BLAST of CmaCh09G009490 vs. NCBI nr
Match: XP_022976533.1 (uncharacterized protein LOC111476900 isoform X1 [Cucurbita maxima])

HSP 1 Score: 725.7 bits (1872), Expect = 2.0e-205
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 0

Query: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60
           MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60

Query: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120
           RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL
Sbjct: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120

Query: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180
           NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI
Sbjct: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180

Query: 181 WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV 240
           WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV
Sbjct: 181 WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV 240

Query: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300
           LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS
Sbjct: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300

Query: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360
           LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT
Sbjct: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360

Query: 361 QI 363
           QI
Sbjct: 361 QI 362

BLAST of CmaCh09G009490 vs. NCBI nr
Match: KAG7024915.1 (hypothetical protein SDJN02_13735, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 716.8 bits (1849), Expect = 9.1e-203
Identity = 357/362 (98.62%), Postives = 360/362 (99.45%), Query Frame = 0

Query: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60
           MLHFR RIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS
Sbjct: 1   MLHFRVRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60

Query: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120
           RLQ+PSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL
Sbjct: 61  RLQNPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120

Query: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180
           NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI
Sbjct: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180

Query: 181 WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV 240
           WMHQPSLQDLKIDE FASPEI+ASMAKGFIVKKLQKR+VDESKELEEYAEFEIEVPPKSV
Sbjct: 181 WMHQPSLQDLKIDEIFASPEIKASMAKGFIVKKLQKRQVDESKELEEYAEFEIEVPPKSV 240

Query: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300
           LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS
Sbjct: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300

Query: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360
           LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT
Sbjct: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360

Query: 361 QI 363
           QI
Sbjct: 361 QI 362

BLAST of CmaCh09G009490 vs. NCBI nr
Match: XP_023535710.1 (uncharacterized protein LOC111797060 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 714.1 bits (1842), Expect = 5.9e-202
Identity = 356/362 (98.34%), Postives = 359/362 (99.17%), Query Frame = 0

Query: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60
           MLHFRFRIRFGNSKNPLNRIPP HLQFKLLQTLAMADVPPS+GRLVCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRIPPIHLQFKLLQTLAMADVPPSTGRLVCPSCSKPARICLCS 60

Query: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120
           RLQ+PSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNV IATVSDVNFEARFTIRLPEL
Sbjct: 61  RLQNPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVAIATVSDVNFEARFTIRLPEL 120

Query: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180
           NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI
Sbjct: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180

Query: 181 WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV 240
           WMHQPSLQDLKIDE FASPEIRASMAKGFIVKKLQKR+VDESKELEEYAEFEIEVPPKSV
Sbjct: 181 WMHQPSLQDLKIDEVFASPEIRASMAKGFIVKKLQKRQVDESKELEEYAEFEIEVPPKSV 240

Query: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300
           LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS
Sbjct: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300

Query: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360
           LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT
Sbjct: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360

Query: 361 QI 363
           QI
Sbjct: 361 QI 362

BLAST of CmaCh09G009490 vs. NCBI nr
Match: XP_022936180.1 (uncharacterized protein LOC111442857 isoform X1 [Cucurbita moschata])

HSP 1 Score: 704.9 bits (1818), Expect = 3.6e-199
Identity = 351/362 (96.96%), Postives = 355/362 (98.07%), Query Frame = 0

Query: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60
           MLHFRFRIRFGNSKNPLNR PPNHLQFKLLQTLAMADVPPS+GRLVCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRFPPNHLQFKLLQTLAMADVPPSTGRLVCPSCSKPARICLCS 60

Query: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120
           RL +PSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIA VSDVNFEARFTIRLPEL
Sbjct: 61  RLHNPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIAPVSDVNFEARFTIRLPEL 120

Query: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSFDHI 180
           NSDEEFSFGNGQNAAQKPQIQVQNRDESLV KPTCTKSDEGAAITATIGKYGVVNSF HI
Sbjct: 121 NSDEEFSFGNGQNAAQKPQIQVQNRDESLVGKPTCTKSDEGAAITATIGKYGVVNSFKHI 180

Query: 181 WMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPPKSV 240
           WMHQPSLQDLKIDE FASPEIR+SMAKGFIVKKLQKR+VDESKELEEYAEFEIEVPPKSV
Sbjct: 181 WMHQPSLQDLKIDEIFASPEIRSSMAKGFIVKKLQKRQVDESKELEEYAEFEIEVPPKSV 240

Query: 241 LLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300
           LLFPSDNAFTVNDDDVH SDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS
Sbjct: 241 LLFPSDNAFTVNDDDVHSSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLDLEKMS 300

Query: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360
           LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT
Sbjct: 301 LYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDERLRITT 360

Query: 361 QI 363
           QI
Sbjct: 361 QI 362

BLAST of CmaCh09G009490 vs. NCBI nr
Match: XP_038897268.1 (uncharacterized protein LOC120085387 [Benincasa hispida])

HSP 1 Score: 596.7 bits (1537), Expect = 1.4e-166
Identity = 311/369 (84.28%), Postives = 326/369 (88.35%), Query Frame = 0

Query: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60
           M+HF FRIRFG+ KNPLNRI PNHLQ KLLQ LAMA+VPPSS R VCPSCSKPARICLCS
Sbjct: 1   MIHFGFRIRFGSPKNPLNRI-PNHLQSKLLQNLAMAEVPPSSRRPVCPSCSKPARICLCS 60

Query: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIRLPEL 120
           RLQS SLENSVGVIILQHSLEK HPLNS+RIAKLGLKNVEIATVSDVNFEARFTIRLPE 
Sbjct: 61  RLQSHSLENSVGVIILQHSLEKNHPLNSARIAKLGLKNVEIATVSDVNFEARFTIRLPEP 120

Query: 121 NS-------DEEFSFGNGQNAAQKPQIQVQNRDESLVDKPTCTKSDEGAAITATIGKYGV 180
           NS       D E SF NGQNA + PQIQ QN  ESLV+K T T +DEGA IT TIGKYGV
Sbjct: 121 NSAAQNFDPDVECSFRNGQNATRNPQIQAQNGGESLVNKLTSTTADEGAIITTTIGKYGV 180

Query: 181 VNSFDHIWMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEI 240
           VNSFDHIWMHQP+LQ+LKI+E  ASPEIRASMAKGFIVKKLQKR++  SKELE+YAEFEI
Sbjct: 181 VNSFDHIWMHQPNLQELKINEILASPEIRASMAKGFIVKKLQKRQLHGSKELEQYAEFEI 240

Query: 241 EVPPKSVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMK 300
           EVPPKSVLLFPS+NAFTVN  DV GSDF VNNLIVLDGTWAKAKRMYNENPWLRLLPHMK
Sbjct: 241 EVPPKSVLLFPSENAFTVN-GDVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHMK 300

Query: 301 LDLEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKD 360
           LDLEKMSLYSEVR QPK GFLSTIESIVYALKVIGD PEGLDDLLDVFESMIGDQRRCKD
Sbjct: 301 LDLEKMSLYSEVRHQPKIGFLSTIESIVYALKVIGDQPEGLDDLLDVFESMIGDQRRCKD 360

Query: 361 ERLRITTQI 363
           ERL IT Q+
Sbjct: 361 ERLNITCQL 367

BLAST of CmaCh09G009490 vs. TAIR 10
Match: AT5G54880.1 (DTW domain-containing protein )

HSP 1 Score: 262.3 bits (669), Expect = 5.7e-70
Identity = 156/358 (43.58%), Postives = 208/358 (58.10%), Query Frame = 0

Query: 44  RLVCPSCSKPARICLCSRLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIAT 103
           R  CPSC KP+++CLC +++SP  +N V + ILQHSLE+KH LNS+RIA+LGLKNV + T
Sbjct: 45  RPTCPSCDKPSQLCLCKKMRSPCFDNQVSITILQHSLERKHALNSTRIARLGLKNVGVTT 104

Query: 104 VSDVNFEARFTIR----------------------LPELNSDEEF---SFGNGQNAAQKP 163
           V DV+ EA F IR                      +  L  D+ F   S GN +N   + 
Sbjct: 105 VFDVHDEAEFLIRVIGSGCSKIDTNHLDSEYRVENVASLELDDSFKLGSSGNVENLESEK 164

Query: 164 QI---------------------QVQNRDESLVDKPTCTKSDEGAAITATIGKYGVVNSF 223
            +                     ++  RD  + DK     S E   I   + K+GV+++ 
Sbjct: 165 NVVLVDHGSLEIAESLNLSQHLSEISYRDSRVRDK---IGSYEEDLIKICMKKHGVISNV 224

Query: 224 DHIWMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEVPP 283
            H  M + +++ L  D   ASP     +AKGF+V K  + K           EFE+EVPP
Sbjct: 225 SHSLMLETNVKVLSFDHILASPAAMDVLAKGFMVTKFSEGK----------QEFELEVPP 284

Query: 284 KSVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLP-HMKLDL 343
            S LLFPS+ +  +N       +  V NLIVLDGTW+KA+R+Y ENPWL+LL  H+KL++
Sbjct: 285 GSALLFPSEESVKINYLK-EKEELKVRNLIVLDGTWSKARRIYLENPWLKLLRCHVKLEI 344

Query: 344 EKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDE 355
           E  SLY EVRRQP+ G LSTIESIVYA+K IG+ PEGLD++L+VFESM+ DQRRCKDE
Sbjct: 345 EGTSLYKEVRRQPRAGCLSTIESIVYAMKEIGEDPEGLDNMLNVFESMVEDQRRCKDE 388

BLAST of CmaCh09G009490 vs. TAIR 10
Match: AT2G41750.1 (DTW domain-containing protein )

HSP 1 Score: 43.5 bits (101), Expect = 4.2e-04
Identity = 20/56 (35.71%), Postives = 31/56 (55.36%), Query Frame = 0

Query: 44  RLVCPSCSKPARICLCSRLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNV 100
           R +C +C +P  ICLC  L +  +  +  +IIL H  E +H LN++ +    L NV
Sbjct: 11  RQICDNCDRPNAICLCHVLPADLIPTNTEIIILHHPHESRHKLNTTPLLTKSLLNV 66

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q473193.1e-0436.51tRNA-uridine aminocarboxypropyltransferase OS=Escherichia coli (strain K12) OX=8... [more]
Q9D0U15.3e-0430.34tRNA-uridine aminocarboxypropyltransferase 2 OS=Mus musculus OX=10090 GN=Dtwd2 P... [more]
Match NameE-valueIdentityDescription
A0A6J1IMF89.5e-206100.00tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1FCX11.7e-19996.96tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A1S3BZW72.2e-15478.80tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo OX=3656 GN=LOC1034950... [more]
A0A0A0LD096.7e-15177.72tRNA-uridine aminocarboxypropyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G8... [more]
A0A5A7T5E23.0e-14380.54tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=119469... [more]
Match NameE-valueIdentityDescription
XP_022976533.12.0e-205100.00uncharacterized protein LOC111476900 isoform X1 [Cucurbita maxima][more]
KAG7024915.19.1e-20398.62hypothetical protein SDJN02_13735, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023535710.15.9e-20298.34uncharacterized protein LOC111797060 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022936180.13.6e-19996.96uncharacterized protein LOC111442857 isoform X1 [Cucurbita moschata][more]
XP_038897268.11.4e-16684.28uncharacterized protein LOC120085387 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G54880.15.7e-7043.58DTW domain-containing protein [more]
AT2G41750.14.2e-0435.71DTW domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005636DTWSMARTSM01144DTW_2acoord: 43..353
e-value: 6.1E-33
score: 125.4
IPR005636DTWPFAMPF03942DTWcoord: 46..348
e-value: 1.3E-51
score: 175.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..149
NoneNo IPR availablePANTHERPTHR21392:SF0DTW DOMAIN-CONTAINING PROTEIN 2coord: 31..357
IPR039262DTW domain-containing protein DTWD2/YfiPPANTHERPTHR21392UNCHARACTERIZEDcoord: 31..357

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh09G009490.1CmaCh09G009490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing
molecular_function GO:0016432 tRNA-uridine aminocarboxypropyltransferase activity