CmaCh09G004090 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh09G004090
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	DNA-directed RNA polymerase subunit beta
Location	Cma_Chr09: 1733920 .. 1737352 (-)
RNA-Seq Expression	CmaCh09G004090
Synteny	CmaCh09G004090

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCCTAGGCATGCTCGACTTCAGAATATGACTTGTTCATCAAGGATGAAAAAGAATATCAGTGTAGAGGTGCTTAATTTACTGTGTAATTTTTACTGTCTTCGTGTCTTTTCTTATCCTTCATACCTAATAAATTGCTAATATTTGTTTGATAACTTGTGGACCCTTAGTTTGTTGAATAAATACCTAATTAGTTATTGGATACTTGTAATGTACTAGGTATATACTCGAAGGTTAGTTACAAGCGATAAAACTGGAAAAGATCAATATGTCGACGAGGATCTTATAAATCAAGATAACAGGGATGTCTTTATAGGCAGGCTACCTGTGATGGTGAATTCTGACCTTTGCTGGATGAAGGATGAGCAGAAACGTGACTCTGAATTTGATCATGGAGGTTATTTTTTAATCAAAGGTGTAGAAAAAGTGAGTACACTTCCCTCCCTCATTTGGCCCCTTTATAGTTATGTATTCTGTAAATAAATTCACTGTTTTCTTAAATTCTACTGTCACCAGACAATCATTTCAAAACTTTTAATGGATTGTTTGTTTGGAGAGTTTTTTATGACCTTGGACAAGTTGACATAAATATGTATCCAGGAGTTCACATTATCTAATAGAGATCATCCTGGTTCATGGTTAATTGGCTGCCTTTTTAAATTTGCATACGCTAAAAATATAAATAAAAAGCCTGTGGCAAAGTATTGAGTTGTGTTCATATTTTTTGAACTTTATGGTATTGAATTATGTATTGGTTTTATTTAATTATTTTTTTAACACAACATTAGCATGCTTATTTTTGAAAATCATGAGTATGGTATTTCTTAGAAAATAGTTGCCTTGGAAAAGAGAGCTATTAGGCCTTAATTATTTGATCGATGTTCAGGCATGTGATCTGCCTCCTGATGGGACATAAAATTTAGTCAAGAAATTTTAGTTCTACAAAATTTTAGTTCCTACTAACTATATTTTGTAAAAATGATTAATTGATACCATCTTTTTCTCTGTATTGCAATTTTGAATAGATATTTATTTCTCTTTCTGCATGCATATTAATCCAAGTAGTTAACTTTTGTTCCTTTTTAACCACTTTCTTTTCATGTTTATTCCAGATATTTATTGCTCGAAAATTCCAAGCCTGAAGAACTAAAAAGCAAGGAGAAAGTCCTCAATGTACATTTTTTCTCCACAAAAGTACCTGTGTGGATTTTATTCTTTGCACCTGGTGTTTCATCGGACAAAGAAATAGTAGATTTAGTTGATTATGGCAGAGATGATCCCATTGTGAACTTGATATGTCATGTTGGTGTGGATACTTTTAACTCCCCAGTTACACTTACATCACTTACGTCGGTGCAAATTTGCAGAGTGGAAACATTGTCATTGGAGGATGTTCGGAATCTGGAGCTGATCACAGTATTAAACTGAAACACACTGTGAAGGGCATGGTTCAGAAAGTTGTGCTGTCGTATGCCTGAGACAGGTACATAGATCTGATTTTAGATGGGCCTGGAACATATTGCGTAGCTAGTGTGTAGCTTCATTAATGGTGCTTTCTATCTTGTATAATTAGAAGCATTATCTCTTGGAGTCTCAAGAGAATTTCCCCTTCACCATCCAGGGGATAGTTCCAGATATTGTTATTAATTCACATTTTTTTTCCTTCGCGACAGACTTCTGGTCAGCTATTGGAAGCTGCTCTTGGGAATGGGATTTCCTCTGGTGGCTGTTTGAAATCTGCGACTCCTTTCTCCACTCCTGTTGATGCTATTACAGACCGGCTTCACAGGTATCTGTTTACCATTGTCGTCATTACTGTTAACTTTTAAATTATTTCGTAGAAAATCTGGCTTTAGTTGATTGAGTAAATGCTTACCGGAACAACTTTCTGACTTCAAATCAGATTATTTTCTTTTAAATATTAAATAAAACCTAGGACTAAAGTGACTTCTTAACAAAGTGTAGCGACTGGTATAATGTTTTAAATTTTCAATTTATAAATTCAATCATGAGCCAGCAGAGGCATATATTTTAATTAAGAGATAAGAGGTTCAATAGTAGTTGAACTCCAAAGAAAAAAAAAAAAAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAGAAAATGCAAGATGATAAAAAAGAAGAAAATCCAGCTCATTTTTTATGTTGAATTTGTGAGCAGCCTGAGTGACTCTTCCCAGATGCTTGTTTGCCAGAAATGCAAGAATGTTGCTAGTGTGATTCAACAATTGGTAGCTAGTGATAATAAGATCAGGGGTCCATATTGTCGGGTTTGCAAGTCAGTCGACGACATTGTGAGGGTAAATGTACCCTATGGAGACAAGTTGTTGTGCCAGGAGCTCTTCAGCATGGTCATCAAGTTCGAAACACAACTTTGTTGATCATGACGCTGATGGAAATAATTGGTTTCCATTTCCTTAAATCTTTTGACTAACTCTGAAGTTGTTCTGTAGGCATGTGTGCTCCAATTTGAAAGAATCTCACGAGGGCCTCTCTTGGTGGGAAGGTAAGTTGTTTCTTATCTCTACTAAATGCTAACATAAATGAAATGAATGGAATTAGTGATGTCGATTCAATACAAGTGAGTTTGGGATGACTTTTATTAGGGTAAATTTGGAAGTTTATTCCATTGAAAAGTAGTTTTGAAAATTTTACCACCGAAAGATAATTTGTTAATTAATCCTTGTAGTGCCTAGTTTTACTTGAAGAAAAGACGTGTTTCCATAAAAATACCACAAAATAGACCAAATCGATTTCTAGTAGCCTTGTAGGAGATTATTTATGCTTCAAAATCAAAAGATTGGTTAAATTGGTTCTCAATTTTAAATTCTATCAGACAATTAAACAGCTTTTTAAGGAAATAATTTTTAAAATTAAAAATTTATAAATTGATGTGGAATTGTAATTTAAAAAATATTTATAGAAGCATAGGCATTAGCTAGGGAGAACTATTTATTAGGGGAGTACATTGTCTCGGTTTGGTTGAAGAATTTTTTTGAACCAACCCAATTCTCTCTTTTCAAGTTGGGTTGTCTTAAAAAAAAAAAATTACTTATTCACAATTTATAATTATTCGATATAATCTAAAATAAAAGATATTAAAAATTAATAAAAAAACATATCAATCTATAATTATTCGATTTATAAGTTGCTCCAAAAATAAGGAAAACATAAAAATAGATCATGAGAATAAAAAATAATAAAAATGATTAGTTTAAAAATGATTAGTTTAGATGGTTCTAAATTATCAGAAAAAATATAATAAAAAATAATTTATGATATAATAATGTTGCACCAAAAAGATTCTGCTTTTGGAAGGGGAACAATAGCTGGAAAAGGCTGGCTAATACCCAATATGTTGAGGAGCAAAAGGAGGAATCCACCTGAGGAAGGGCTCACATCTGATTAG

mRNA sequence

ATGCTTCCTAGGCATGCTCGACTTCAGAATATGACTTGTTCATCAAGGATGAAAAAGAATATCAGTGTAGAGGTATATACTCGAAGGTTAGTTACAAGCGATAAAACTGGAAAAGATCAATATGTCGACGAGGATCTTATAAATCAAGATAACAGGGATGTCTTTATAGGCAGGCTACCTGTGATGAAAAAATATTTATTGCTCGAAAATTCCAAGCCTGAAGAACTAAAAAGCAAGGAGAAAGTCCTCAATAGTGGAAACATTGTCATTGGAGGATGTTCGGAATCTGGAGCTGATCACAGTATTAAACTGAAACACACTGTGAAGGGCATGACTTCTGGTCAGCTATTGGAAGCTGCTCTTGGGAATGGGATTTCCTCTGGTGGCTGTTTGAAATCTGCGACTCCTTTCTCCACTCCTGTTGATGCTATTACAGACCGGCTTCACAGCCTGAGTGACTCTTCCCAGATGCTTGTTTGCCAGAAATGCAAGAATGTTGCTAGTGTGATTCAACAATTGGTAGCTAGTGATAATAAGATCAGGGGTCCATATTGTCGGGTTTGCAAGTCAGTCGACGACATTGTGAGGGTAAATGTACCCTATGGAGACAAGTTGTTGTGCCAGGAGCTCTTCAGCATGGTCATCAAGCATGTGTGCTCCAATTTGAAAGAATCTCACGAGGGCCTCTCTTGGTGGGAAGATTCTGCTTTTGGAAGGGGAACAATAGCTGGAAAAGGCTGGCTAATACCCAATATGTTGAGGAGCAAAAGGAGGAATCCACCTGAGGAAGGGCTCACATCTGATTAG

Coding sequence (CDS)

ATGCTTCCTAGGCATGCTCGACTTCAGAATATGACTTGTTCATCAAGGATGAAAAAGAATATCAGTGTAGAGGTATATACTCGAAGGTTAGTTACAAGCGATAAAACTGGAAAAGATCAATATGTCGACGAGGATCTTATAAATCAAGATAACAGGGATGTCTTTATAGGCAGGCTACCTGTGATGAAAAAATATTTATTGCTCGAAAATTCCAAGCCTGAAGAACTAAAAAGCAAGGAGAAAGTCCTCAATAGTGGAAACATTGTCATTGGAGGATGTTCGGAATCTGGAGCTGATCACAGTATTAAACTGAAACACACTGTGAAGGGCATGACTTCTGGTCAGCTATTGGAAGCTGCTCTTGGGAATGGGATTTCCTCTGGTGGCTGTTTGAAATCTGCGACTCCTTTCTCCACTCCTGTTGATGCTATTACAGACCGGCTTCACAGCCTGAGTGACTCTTCCCAGATGCTTGTTTGCCAGAAATGCAAGAATGTTGCTAGTGTGATTCAACAATTGGTAGCTAGTGATAATAAGATCAGGGGTCCATATTGTCGGGTTTGCAAGTCAGTCGACGACATTGTGAGGGTAAATGTACCCTATGGAGACAAGTTGTTGTGCCAGGAGCTCTTCAGCATGGTCATCAAGCATGTGTGCTCCAATTTGAAAGAATCTCACGAGGGCCTCTCTTGGTGGGAAGATTCTGCTTTTGGAAGGGGAACAATAGCTGGAAAAGGCTGGCTAATACCCAATATGTTGAGGAGCAAAAGGAGGAATCCACCTGAGGAAGGGCTCACATCTGATTAG

Protein sequence

MLPRHARLQNMTCSSRMKKNISVEVYTRRLVTSDKTGKDQYVDEDLINQDNRDVFIGRLPVMKKYLLLENSKPEELKSKEKVLNSGNIVIGGCSESGADHSIKLKHTVKGMTSGQLLEAALGNGISSGGCLKSATPFSTPVDAITDRLHSLSDSSQMLVCQKCKNVASVIQQLVASDNKIRGPYCRVCKSVDDIVRVNVPYGDKLLCQELFSMVIKHVCSNLKESHEGLSWWEDSAFGRGTIAGKGWLIPNMLRSKRRNPPEEGLTSD

Homology

[Close]

BLAST of CmaCh09G004090 vs. ExPASy Swiss-Prot
Match: Q9LV32 (DNA-directed RNA polymerase D subunit 2b OS=Arabidopsis thaliana OX=3702 GN=NRPD2b PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-19
Identity = 47/70 (67.14%), Postives = 56/70 (80.00%), Query Frame = 0


Query: 146  DRLHSLSDSSQMLVCQKCKNVASVIQQLVASDNKIRGPYCRVCKSVDDIVRVNVPYGDKL 205
            +RL +LSDSSQM +C+KCK  A+VI++  +S  KIRGPYCRVC S D +VRV VPYG KL
Sbjct: 1041 ERLFTLSDSSQMHICRKCKTYANVIERTPSSGRKIRGPYCRVCASSDHVVRVYVPYGAKL 1100

Query: 206  LCQELFSMVI 216
            LCQELFSM I
Sbjct: 1101 LCQELFSMGI 1110

HSP 2 Score: 48.5 bits (114), Expect = 1.4e-04
Identity = 21/37 (56.76%), Postives = 30/37 (81.08%), Query Frame = 0


Query: 83  LNSGNIVIGGCSESGADHSIKLKHTVKGMTSGQLLEA 120
           +++G+IVIG C+ESGADHSIKLKHT +G+    +L +
Sbjct: 800 MSTGDIVIGRCTESGADHSIKLKHTERGIVQKVVLSS 836

[Close]

BLAST of CmaCh09G004090 vs. ExPASy Swiss-Prot
Match: Q9LK40 (DNA-directed RNA polymerases IV and V subunit 2 OS=Arabidopsis thaliana OX=3702 GN=NRPD2 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.5e-19
Identity = 47/70 (67.14%), Postives = 56/70 (80.00%), Query Frame = 0


Query: 146  DRLHSLSDSSQMLVCQKCKNVASVIQQLVASDNKIRGPYCRVCKSVDDIVRVNVPYGDKL 205
            +RL +LSDSSQM +C+KCK  A+VI++  +S  KIRGPYCRVC S D +VRV VPYG KL
Sbjct: 1094 ERLFTLSDSSQMHICRKCKTYANVIERTPSSGRKIRGPYCRVCVSSDHVVRVYVPYGAKL 1153

Query: 206  LCQELFSMVI 216
            LCQELFSM I
Sbjct: 1154 LCQELFSMGI 1163

HSP 2 Score: 69.3 bits (168), Expect = 7.4e-11
Identity = 35/73 (47.95%), Postives = 52/73 (71.23%), Query Frame = 0


Query: 2   LPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGRL 61
           LP HARLQNMT S+R+K N+ VEV+   +V SD  KTG+D YV++ +++   +D+ IG +
Sbjct: 117 LPWHARLQNMTYSARIKVNVQVEVFKNTVVKSDKFKTGQDNYVEKKILDVKKQDILIGSI 176

Query: 62  PVMKKYLLLENSK 73
           PVM K +L + S+
Sbjct: 177 PVMVKSILCKTSE 189

HSP 3 Score: 48.5 bits (114), Expect = 1.4e-04
Identity = 21/37 (56.76%), Postives = 30/37 (81.08%), Query Frame = 0


Query: 83  LNSGNIVIGGCSESGADHSIKLKHTVKGMTSGQLLEA 120
           +++G+IVIG C+ESGADHSIKLKHT +G+    +L +
Sbjct: 854 MSTGDIVIGRCTESGADHSIKLKHTERGIVQKVVLSS 890

[Close]

BLAST of CmaCh09G004090 vs. ExPASy Swiss-Prot
Match: Q42877 (DNA-directed RNA polymerase II subunit RPB2 OS=Solanum lycopersicum OX=4081 GN=RPB2 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 7.9e-05
Identity = 28/73 (38.36%), Postives = 43/73 (58.90%), Query Frame = 0


Query: 144  ITDRLHSLSDSSQMLVCQKCKNVASVIQQLVASDNKIRGPY-CRVCKSVDDIVRVNVPYG 203
            + +RL   SD+ ++ VC++C         L+A  N  +  + CR CK+  DIV+V++PY 
Sbjct: 1107 LKERLFDQSDAYRVHVCERC--------GLIAIANLKKNSFECRGCKNKTDIVQVHIPYA 1166

Query: 204  DKLLCQELFSMVI 216
             KLL QEL +M I
Sbjct: 1167 CKLLFQELMAMAI 1171

[Close]

BLAST of CmaCh09G004090 vs. ExPASy Swiss-Prot
Match: P38420 (DNA-directed RNA polymerase II subunit 2 OS=Arabidopsis thaliana OX=3702 GN=NRPB2 PE=1 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 2.3e-04
Identity = 29/73 (39.73%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 144  ITDRLHSLSDSSQMLVCQKCKNVASVIQQLVASDNKIRGPY-CRVCKSVDDIVRVNVPYG 203
            + +RL   SD+ ++ VC+ C         L+A  N  +  + CR CK+  DIV+V +PY 
Sbjct: 1108 LKERLFDQSDAYRVHVCEVC--------GLIAIANLKKNSFECRGCKNKTDIVQVYIPYA 1167

Query: 204  DKLLCQELFSMVI 216
             KLL QEL SM I
Sbjct: 1168 CKLLFQELMSMAI 1172

[Close]

BLAST of CmaCh09G004090 vs. ExPASy TrEMBL
Match: A0A6J1C950 (DNA-directed RNA polymerase subunit beta OS=Momordica charantia OX=3673 GN=LOC111009271 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 8.7e-31
Identity = 111/299 (37.12%), Postives = 119/299 (39.80%), Query Frame = 0


Query: 83   LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
            L SG+IVIG C+ESGADHSIKLKHT KGM                               
Sbjct: 887  LQSGDIVIGRCAESGADHSIKLKHTEKGMVQKVVLSSNDDGKNYAVVCLRQVRSPCLGDK 946

Query: 143  ------------------------------------------TSGQLLEAALGNGISSGG 202
                                                      T GQLLEAALG GI++GG
Sbjct: 947  FSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALGKGIAAGG 1006

Query: 203  CLKSATPFSTP-VDAITDRLH--------------------------------------- 216
             LK ATPFSTP VDAITD+LH                                       
Sbjct: 1007 SLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMA 1066

[Close]

BLAST of CmaCh09G004090 vs. ExPASy TrEMBL
Match: A0A6J1C950 (DNA-directed RNA polymerase subunit beta OS=Momordica charantia OX=3673 GN=LOC111009271 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 2.2e-18
Identity = 52/64 (81.25%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1   MLPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGR 60
           MLPRHARLQNMT SSRMK NI+VEVYT+RLV SD  KTGKDQYVD+DL++QDNR++FIGR
Sbjct: 135 MLPRHARLQNMTYSSRMKMNINVEVYTQRLVRSDKFKTGKDQYVDKDLLSQDNREIFIGR 194

Query: 61  LPVM 63
           LPVM
Sbjct: 195 LPVM 198

HSP 2 Score: 144.1 bits (362), Expect = 8.7e-31
Identity = 111/299 (37.12%), Postives = 119/299 (39.80%), Query Frame = 0


Query: 83   LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
            L SG+IVIG C+ESGADHSIKLKHT KGM                               
Sbjct: 886  LQSGDIVIGRCAESGADHSIKLKHTEKGMVQKVVLSSNDDGKNFAVVSLRQVRSPCLGDK 945

Query: 143  ------------------------------------------TSGQLLEAALGNGISSGG 202
                                                      T GQLLEAALG GI+SGG
Sbjct: 946  FSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALGKGIASGG 1005

Query: 203  CLKSATPFSTP-VDAITDRLH--------------------------------------- 216
             LK ATPFSTP VDAITD+LH                                       
Sbjct: 1006 SLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMA 1065

[Close]

BLAST of CmaCh09G004090 vs. ExPASy TrEMBL
Match: A0A6J1FHZ2 (DNA-directed RNA polymerase subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111445938 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 2.2e-18
Identity = 52/64 (81.25%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1   MLPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGR 60
           MLPRHARLQNMT SSRMK NI+VEVYT+RLV SD  KTG+DQYVD+DL++QDNRD+FIGR
Sbjct: 134 MLPRHARLQNMTYSSRMKMNINVEVYTQRLVRSDKFKTGQDQYVDKDLLSQDNRDIFIGR 193

Query: 61  LPVM 63
           LPVM
Sbjct: 194 LPVM 197

HSP 2 Score: 144.1 bits (362), Expect = 8.7e-31
Identity = 111/299 (37.12%), Postives = 119/299 (39.80%), Query Frame = 0


Query: 83   LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
            L SG+IVIG C+ESGADHSIKLKHT KGM                               
Sbjct: 890  LQSGDIVIGRCAESGADHSIKLKHTEKGMVQKVVLSSNDDGKNYAVVSLRQVRSPCLGDK 949

Query: 143  ------------------------------------------TSGQLLEAALGNGISSGG 202
                                                      T GQLLEAALG GI++GG
Sbjct: 950  FSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALGKGIAAGG 1009

Query: 203  CLKSATPFSTP-VDAITDRLH--------------------------------------- 216
             LK ATPFSTP VDAITD+LH                                       
Sbjct: 1010 SLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMA 1069

[Close]

BLAST of CmaCh09G004090 vs. ExPASy TrEMBL
Match: A0A5D3D8Y5 (DNA-directed RNA polymerase subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001250 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 3.9e-15
Identity = 49/66 (74.24%), Postives = 57/66 (86.36%), Query Frame = 0


Query: 1   MLPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLIN--QDNRDVFI 60
           MLPRHARLQNMT SSRMK NIS+E+YT++LV+SD  KTGKDQYVD++ +    DNRDVFI
Sbjct: 136 MLPRHARLQNMTYSSRMKINISLEIYTQKLVSSDKFKTGKDQYVDKEPVEGASDNRDVFI 195

Query: 61  GRLPVM 63
           GRLPVM
Sbjct: 196 GRLPVM 201

HSP 2 Score: 144.1 bits (362), Expect = 8.7e-31
Identity = 111/299 (37.12%), Postives = 119/299 (39.80%), Query Frame = 0


Query: 83   LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
            L SG+IVIG C+ESGADHSIKLKHT KGM                               
Sbjct: 886  LQSGDIVIGRCAESGADHSIKLKHTEKGMVQKVVLSSNDDGKNFAVVSLRQVRSPCLGDK 945

Query: 143  ------------------------------------------TSGQLLEAALGNGISSGG 202
                                                      T GQLLEAALG GI+SGG
Sbjct: 946  FSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALGKGIASGG 1005

Query: 203  CLKSATPFSTP-VDAITDRLH--------------------------------------- 216
             LK ATPFSTP VDAITD+LH                                       
Sbjct: 1006 SLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMA 1065

[Close]

BLAST of CmaCh09G004090 vs. ExPASy TrEMBL
Match: A0A6J1IXN3 (DNA-directed RNA polymerase subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111480863 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 5.0e-18
Identity = 51/64 (79.69%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1   MLPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGR 60
           +LPRHARLQNMT SSRMK NI+VEVYT+RLV SD  KTG+DQYVD+DL++QDNRD+FIGR
Sbjct: 134 LLPRHARLQNMTYSSRMKMNINVEVYTQRLVRSDKFKTGQDQYVDKDLLSQDNRDIFIGR 193

Query: 61  LPVM 63
           LPVM
Sbjct: 194 LPVM 197

HSP 2 Score: 144.1 bits (362), Expect = 8.7e-31
Identity = 111/299 (37.12%), Postives = 119/299 (39.80%), Query Frame = 0


Query: 83   LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
            L SG+IVIG C+ESGADHSIKLKHT KGM                               
Sbjct: 890  LQSGDIVIGRCAESGADHSIKLKHTEKGMVQKVVLSSNDDGKNYAVVSLRQVRSPCLGDK 949

Query: 143  ------------------------------------------TSGQLLEAALGNGISSGG 202
                                                      T GQLLEAALG GI++GG
Sbjct: 950  FSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALGKGIAAGG 1009

Query: 203  CLKSATPFSTP-VDAITDRLH--------------------------------------- 216
             LK ATPFSTP VDAITD+LH                                       
Sbjct: 1010 SLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMA 1069

[Close]

BLAST of CmaCh09G004090 vs. NCBI nr
Match: KAG7024417.1 (DNA-directed RNA polymerase D subunit 2b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 215.7 bits (548), Expect = 4.9e-52
Identity = 119/176 (67.61%), Postives = 126/176 (71.59%), Query Frame = 0


Query: 83  LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
           L SGNIVIGGC+ESGADHSIKLKHTVKGM                               
Sbjct: 248 LQSGNIVIGGCAESGADHSIKLKHTVKGMVQKVMLSFGLLVLGVSREFPLHHPGIVPDIV 307

Query: 143 ----------TSGQLLEAALGNGISSGGCLKSATPFSTP-VDAITDRLHSLSDSSQMLVC 202
                     TSGQLLEAALG GI+SGGCLKSATPFSTP VDAITDRLHSLSDSSQM V 
Sbjct: 308 INSCFFPSRQTSGQLLEAALGKGIASGGCLKSATPFSTPSVDAITDRLHSLSDSSQMHVS 367

Query: 203 QKCKNVASVIQQLVASDNKIRGPYCRVCKSVDDIVRVNVPYGDKLLCQELFSMVIK 217
           QKCKN ASVIQQ VAS +KIRGPYCRVC+S+DD+VRVNVPYG KLLCQELFSMVI+
Sbjct: 368 QKCKNAASVIQQSVASGHKIRGPYCRVCESIDDVVRVNVPYGAKLLCQELFSMVIE 423

[Close]

BLAST of CmaCh09G004090 vs. NCBI nr
Match: XP_022940261.1 (DNA-directed RNA polymerases IV and V subunit 2-like [Cucurbita moschata] >XP_022940262.1 DNA-directed RNA polymerases IV and V subunit 2-like [Cucurbita moschata])

HSP 1 Score: 144.1 bits (362), Expect = 1.8e-30
Identity = 111/299 (37.12%), Postives = 119/299 (39.80%), Query Frame = 0


Query: 83   LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
            L SG+IVIG C+ESGADHSIKLKHT KGM                               
Sbjct: 886  LQSGDIVIGRCAESGADHSIKLKHTEKGMVQKVVLSSNDDGKNFAVVSLRQVRSPCLGDK 945

Query: 143  ------------------------------------------TSGQLLEAALGNGISSGG 202
                                                      T GQLLEAALG GI+SGG
Sbjct: 946  FSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALGKGIASGG 1005

Query: 203  CLKSATPFSTP-VDAITDRLH--------------------------------------- 216
             LK ATPFSTP VDAITD+LH                                       
Sbjct: 1006 SLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMA 1065

[Close]

BLAST of CmaCh09G004090 vs. NCBI nr
Match: XP_022940261.1 (DNA-directed RNA polymerases IV and V subunit 2-like [Cucurbita moschata] >XP_022940262.1 DNA-directed RNA polymerases IV and V subunit 2-like [Cucurbita moschata])

HSP 1 Score: 102.8 bits (255), Expect = 4.6e-18
Identity = 52/64 (81.25%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1   MLPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGR 60
           MLPRHARLQNMT SSRMK NI+VEVYT+RLV SD  KTG+DQYVD+DL++QDNRD+FIGR
Sbjct: 134 MLPRHARLQNMTYSSRMKMNINVEVYTQRLVRSDKFKTGQDQYVDKDLLSQDNRDIFIGR 193

Query: 61  LPVM 63
           LPVM
Sbjct: 194 LPVM 197

HSP 2 Score: 144.1 bits (362), Expect = 1.8e-30
Identity = 111/299 (37.12%), Postives = 119/299 (39.80%), Query Frame = 0


Query: 83   LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
            L SG+IVIG C+ESGADHSIKLKHT KGM                               
Sbjct: 887  LQSGDIVIGRCAESGADHSIKLKHTEKGMVQKVVLSSNDDGKNYAVVCLRQVRSPCLGDK 946

Query: 143  ------------------------------------------TSGQLLEAALGNGISSGG 202
                                                      T GQLLEAALG GI++GG
Sbjct: 947  FSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALGKGIAAGG 1006

Query: 203  CLKSATPFSTP-VDAITDRLH--------------------------------------- 216
             LK ATPFSTP VDAITD+LH                                       
Sbjct: 1007 SLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMA 1066

[Close]

BLAST of CmaCh09G004090 vs. NCBI nr
Match: XP_022138042.1 (DNA-directed RNA polymerases IV and V subunit 2-like [Momordica charantia] >XP_022138109.1 DNA-directed RNA polymerases IV and V subunit 2-like [Momordica charantia])

HSP 1 Score: 102.8 bits (255), Expect = 4.6e-18
Identity = 52/64 (81.25%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1   MLPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGR 60
           MLPRHARLQNMT SSRMK NI+VEVYT+RLV SD  KTGKDQYVD+DL++QDNR++FIGR
Sbjct: 135 MLPRHARLQNMTYSSRMKMNINVEVYTQRLVRSDKFKTGKDQYVDKDLLSQDNREIFIGR 194

Query: 61  LPVM 63
           LPVM
Sbjct: 195 LPVM 198

HSP 2 Score: 144.1 bits (362), Expect = 1.8e-30
Identity = 111/299 (37.12%), Postives = 119/299 (39.80%), Query Frame = 0


Query: 83   LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
            L SG+IVIG C+ESGADHSIKLKHT KGM                               
Sbjct: 886  LQSGDIVIGRCAESGADHSIKLKHTEKGMVQKVVLSSNDDGKNFAVVSLRQVRSPCLGDK 945

Query: 143  ------------------------------------------TSGQLLEAALGNGISSGG 202
                                                      T GQLLEAALG GI+SGG
Sbjct: 946  FSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALGKGIASGG 1005

Query: 203  CLKSATPFSTP-VDAITDRLH--------------------------------------- 216
             LK ATPFSTP VDAITD+LH                                       
Sbjct: 1006 SLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMA 1065

[Close]

BLAST of CmaCh09G004090 vs. NCBI nr
Match: XP_023524029.1 (DNA-directed RNA polymerases IV and V subunit 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 102.8 bits (255), Expect = 4.6e-18
Identity = 52/64 (81.25%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1   MLPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGR 60
           MLPRHARLQNMT SSRMK NI+VEVYT+RLV SD  KTG+DQYVD+DL++QDNRD+FIGR
Sbjct: 134 MLPRHARLQNMTYSSRMKMNINVEVYTQRLVRSDKFKTGQDQYVDKDLLSQDNRDIFIGR 193

Query: 61  LPVM 63
           LPVM
Sbjct: 194 LPVM 197

HSP 2 Score: 144.1 bits (362), Expect = 1.8e-30
Identity = 111/299 (37.12%), Postives = 119/299 (39.80%), Query Frame = 0


Query: 83   LNSGNIVIGGCSESGADHSIKLKHTVKGM------------------------------- 142
            L SG+IVIG C+ESGADHSIKLKHT KGM                               
Sbjct: 886  LQSGDIVIGRCAESGADHSIKLKHTEKGMVQKVVLSSNDDGKNFAVVSLRQVRSPCLGDK 945

Query: 143  ------------------------------------------TSGQLLEAALGNGISSGG 202
                                                      T GQLLEAALG GI+SGG
Sbjct: 946  FSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALGKGIASGG 1005

Query: 203  CLKSATPFSTP-VDAITDRLH--------------------------------------- 216
             LK ATPFSTP VDAITD+LH                                       
Sbjct: 1006 SLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMA 1065

[Close]

BLAST of CmaCh09G004090 vs. TAIR 10
Match: AT3G18090.1 (nuclear RNA polymerase D2B )

HSP 1 Score: 98.6 bits (244), Expect = 8.1e-21
Identity = 47/70 (67.14%), Postives = 56/70 (80.00%), Query Frame = 0


Query: 146  DRLHSLSDSSQMLVCQKCKNVASVIQQLVASDNKIRGPYCRVCKSVDDIVRVNVPYGDKL 205
            +RL +LSDSSQM +C+KCK  A+VI++  +S  KIRGPYCRVC S D +VRV VPYG KL
Sbjct: 977  ERLFTLSDSSQMHICRKCKTYANVIERTPSSGRKIRGPYCRVCASSDHVVRVYVPYGAKL 1036

Query: 206  LCQELFSMVI 216
            LCQELFSM I
Sbjct: 1037 LCQELFSMGI 1046

HSP 2 Score: 58.9 bits (141), Expect = 7.1e-09
Identity = 29/68 (42.65%), Postives = 49/68 (72.06%), Query Frame = 0


Query: 7  RLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGRLPVMKK 66
          RLQNMT S+R+K N  VEV+ + ++  D  KTG+D+YV++++++   +D+ IG +PVM K
Sbjct: 4  RLQNMTYSARIKVNAQVEVFKKIVIKHDKFKTGQDEYVEKEILDVKKQDILIGSIPVMVK 63

Query: 67 YLLLENSK 73
           +L + S+
Sbjct: 64 SVLCKTSE 71

HSP 3 Score: 48.5 bits (114), Expect = 9.6e-06
Identity = 21/37 (56.76%), Postives = 30/37 (81.08%), Query Frame = 0


Query: 83  LNSGNIVIGGCSESGADHSIKLKHTVKGMTSGQLLEA 120
           +++G+IVIG C+ESGADHSIKLKHT +G+    +L +
Sbjct: 736 MSTGDIVIGRCTESGADHSIKLKHTERGIVQKVVLSS 772

[Close]

BLAST of CmaCh09G004090 vs. TAIR 10
Match: AT3G23780.1 (nuclear RNA polymerase D2A )

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-20
Identity = 47/70 (67.14%), Postives = 56/70 (80.00%), Query Frame = 0


Query: 146  DRLHSLSDSSQMLVCQKCKNVASVIQQLVASDNKIRGPYCRVCKSVDDIVRVNVPYGDKL 205
            +RL +LSDSSQM +C+KCK  A+VI++  +S  KIRGPYCRVC S D +VRV VPYG KL
Sbjct: 1094 ERLFTLSDSSQMHICRKCKTYANVIERTPSSGRKIRGPYCRVCVSSDHVVRVYVPYGAKL 1153

Query: 206  LCQELFSMVI 216
            LCQELFSM I
Sbjct: 1154 LCQELFSMGI 1163

HSP 2 Score: 69.3 bits (168), Expect = 5.3e-12
Identity = 35/73 (47.95%), Postives = 52/73 (71.23%), Query Frame = 0


Query: 2   LPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGRL 61
           LP HARLQNMT S+R+K N+ VEV+   +V SD  KTG+D YV++ +++   +D+ IG +
Sbjct: 117 LPWHARLQNMTYSARIKVNVQVEVFKNTVVKSDKFKTGQDNYVEKKILDVKKQDILIGSI 176

Query: 62  PVMKKYLLLENSK 73
           PVM K +L + S+
Sbjct: 177 PVMVKSILCKTSE 189

HSP 3 Score: 48.5 bits (114), Expect = 9.6e-06
Identity = 21/37 (56.76%), Postives = 30/37 (81.08%), Query Frame = 0


Query: 83  LNSGNIVIGGCSESGADHSIKLKHTVKGMTSGQLLEA 120
           +++G+IVIG C+ESGADHSIKLKHT +G+    +L +
Sbjct: 854 MSTGDIVIGRCTESGADHSIKLKHTERGIVQKVVLSS 890

[Close]

BLAST of CmaCh09G004090 vs. TAIR 10
Match: AT3G23780.2 (nuclear RNA polymerase D2A )

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-20
Identity = 47/70 (67.14%), Postives = 56/70 (80.00%), Query Frame = 0


Query: 146  DRLHSLSDSSQMLVCQKCKNVASVIQQLVASDNKIRGPYCRVCKSVDDIVRVNVPYGDKL 205
            +RL +LSDSSQM +C+KCK  A+VI++  +S  KIRGPYCRVC S D +VRV VPYG KL
Sbjct: 1094 ERLFTLSDSSQMHICRKCKTYANVIERTPSSGRKIRGPYCRVCVSSDHVVRVYVPYGAKL 1153

Query: 206  LCQELFSMVI 216
            LCQELFSM I
Sbjct: 1154 LCQELFSMGI 1163

HSP 2 Score: 69.3 bits (168), Expect = 5.3e-12
Identity = 35/73 (47.95%), Postives = 52/73 (71.23%), Query Frame = 0


Query: 2   LPRHARLQNMTCSSRMKKNISVEVYTRRLVTSD--KTGKDQYVDEDLINQDNRDVFIGRL 61
           LP HARLQNMT S+R+K N+ VEV+   +V SD  KTG+D YV++ +++   +D+ IG +
Sbjct: 117 LPWHARLQNMTYSARIKVNVQVEVFKNTVVKSDKFKTGQDNYVEKKILDVKKQDILIGSI 176

Query: 62  PVMKKYLLLENSK 73
           PVM K +L + S+
Sbjct: 177 PVMVKSILCKTSE 189

HSP 3 Score: 48.5 bits (114), Expect = 9.6e-06
Identity = 21/37 (56.76%), Postives = 30/37 (81.08%), Query Frame = 0


Query: 83  LNSGNIVIGGCSESGADHSIKLKHTVKGMTSGQLLEA 120
           +++G+IVIG C+ESGADHSIKLKHT +G+    +L +
Sbjct: 854 MSTGDIVIGRCTESGADHSIKLKHTERGIVQKVVLSS 890

[Close]

BLAST of CmaCh09G004090 vs. TAIR 10
Match: AT4G21710.1 (DNA-directed RNA polymerase family protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.6e-05
Identity = 29/73 (39.73%), Postives = 41/73 (56.16%), Query Frame = 0


Query: 144  ITDRLHSLSDSSQMLVCQKCKNVASVIQQLVASDNKIRGPY-CRVCKSVDDIVRVNVPYG 203
            + +RL   SD+ ++ VC+ C         L+A  N  +  + CR CK+  DIV+V +PY 
Sbjct: 1108 LKERLFDQSDAYRVHVCEVC--------GLIAIANLKKNSFECRGCKNKTDIVQVYIPYA 1167

Query: 204  DKLLCQELFSMVI 216
             KLL QEL SM I
Sbjct: 1168 CKLLFQELMSMAI 1172

The following BLAST results are available for this feature:

BLAST of CmaCh09G004090 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LV32	1.1e-19	67.14	DNA-directed RNA polymerase D subunit 2b OS=Arabidopsis thaliana OX=3702 GN=NRPD...	[more]
Q9LK40	1.5e-19	67.14	DNA-directed RNA polymerases IV and V subunit 2 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q42877	7.9e-05	38.36	DNA-directed RNA polymerase II subunit RPB2 OS=Solanum lycopersicum OX=4081 GN=R...	[more]
P38420	2.3e-04	39.73	DNA-directed RNA polymerase II subunit 2 OS=Arabidopsis thaliana OX=3702 GN=NRPB...	[more]

BLAST of CmaCh09G004090 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C950	8.7e-31	37.12	DNA-directed RNA polymerase subunit beta OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1C950	2.2e-18	81.25	DNA-directed RNA polymerase subunit beta OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1FHZ2	2.2e-18	81.25	DNA-directed RNA polymerase subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A5D3D8Y5	3.9e-15	74.24	DNA-directed RNA polymerase subunit beta OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A6J1IXN3	5.0e-18	79.69	DNA-directed RNA polymerase subunit beta OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]

Pages

BLAST of CmaCh09G004090 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024417.1	4.9e-52	67.61	DNA-directed RNA polymerase D subunit 2b, partial [Cucurbita argyrosperma subsp....	[more]
XP_022940261.1	1.8e-30	37.12	DNA-directed RNA polymerases IV and V subunit 2-like [Cucurbita moschata] >XP_02...	[more]
XP_022940261.1	4.6e-18	81.25	DNA-directed RNA polymerases IV and V subunit 2-like [Cucurbita moschata] >XP_02...	[more]
XP_022138042.1	4.6e-18	81.25	DNA-directed RNA polymerases IV and V subunit 2-like [Momordica charantia] >XP_0...	[more]
XP_023524029.1	4.6e-18	81.25	DNA-directed RNA polymerases IV and V subunit 2-like [Cucurbita pepo subsp. pepo...	[more]

Pages

BLAST of CmaCh09G004090 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18090.1	8.1e-21	67.14	nuclear RNA polymerase D2B	[more]
AT3G23780.1	1.1e-20	67.14	nuclear RNA polymerase D2A	[more]
AT3G23780.2	1.1e-20	67.14	nuclear RNA polymerase D2A	[more]
AT4G21710.1	1.6e-05	39.73	DNA-directed RNA polymerase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.1800.10	RNA polymerase alpha subunit dimerisation domain	coord: 141..214 e-value: 2.2E-14 score: 55.2
None	No IPR available	PANTHER	PTHR20856:SF27	DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA	coord: 144..215 coord: 1..64 coord: 112..148
None	No IPR available	PANTHER	PTHR20856:SF27	DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA	coord: 83..112
None	No IPR available	SUPERFAMILY	64484	beta and beta-prime subunits of DNA dependent RNA-polymerase	coord: 83..217
IPR007641	RNA polymerase Rpb2, domain 7	PFAM	PF04560	RNA_pol_Rpb2_7	coord: 144..216 e-value: 1.3E-12 score: 47.9
IPR015712	DNA-directed RNA polymerase, subunit 2	PANTHER	PTHR20856	DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2	coord: 83..112
IPR015712	DNA-directed RNA polymerase, subunit 2	PANTHER	PTHR20856	DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2	coord: 144..215 coord: 1..64 coord: 112..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh09G004090.1	CmaCh09G004090.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0032549	ribonucleoside binding