CmaCh08G007050 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh08G007050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionrop guanine nucleotide exchange factor 12-like
LocationCma_Chr08: 4304392 .. 4307601 (+)
RNA-Seq ExpressionCmaCh08G007050
SyntenyCmaCh08G007050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAAGGGGAAGGGAGGGAGGGGGAGAGAGAGCAAAACCAAAATCTTTTCCTTATTCCTCAGTTCAAAGGAGGTAAAGAGAAGCAAAACAAAACCATCCCACCACCATTTTTCTTGTTTTTTGTCTAAAAATCCTGCTTCTCTTCCTCCTTTTTTTTTTCTTAATTCCCTTCCTTTTCTCTCTATGCTGTCTGAAACCCAGAAGCAATTGATCACCCAGTTGCCGGTTTGTTGTCCCTTGCGTTTTCCGTCAAAAGTTTCATCAAGAAACTGAAGATGATTCATGCTATGGAGCAGGAGCACGAAGGTTTCAAATCCAAATTATGTCATTTCAAAGGAACGCACGAGAATTCTGGCAGACATGCCAAGAGCTTGAGCATCGAGGGTGCCGGCATGTTGGACTCTCTTGTCGACGAGGATACATCAAGAAGCCAAGGATCGACGCCAGTTCACAACAACTCTGCAGAGAAGCCGCTGACACCAACCAATCGACCTGAAAAGTCTCTGAAGCTGAAGTTGACCAAGGATGAAGATGCTGTCACTGAGAAGGAACCCAGAGACAAGAAGGCCTTAGGTTCAATTCCTAGCCATTCTTTTAAGATCTAATATTCATTTAATCACTACTCACCTTAATCAACCTATACATAACTCGGAGGGATTTGAATCTTTGACCTCTTGCTCAAAGGTACATGTCAATCAAAGCTAAGGCACATATTCAAGCCACATTATTCATTTGTTAAAGTTAATAACTAAAGATTTATTAAAATTAAGACATAACAATGGTTAGTTTTTTTTAGAAATTTTGGATTCAAAATAGGCATATTGAGGGTTAAGGGGGCTAAAATAGAACAAACATAAAGTTCGAGGACCGTAACGAGCCATGTGAAGTTAAGGTATCAAAATAGGTATTGTTCATTCTAGGAGGAATGCATTTTGTTTGATTCTGCAATTAACATTCTCCTCCAATCTCGGGTTATAATTCAGAAATGGAACAAATGAAAGAGAGGTTTGCAAAGTTACTCTTGGGAGAAGACATGTCTGGGGGAGGAAAGGGTGTTTCCTCAGCCTTGGCATTGTCTAATGCAATCACAAACCTTGCTGGTATGTACTTAGAATTTTCTAACATGGTAACTGAGAAATCTCTGTTGGGATCTAAACTTCTGTCAATGTTGCAGCTTCTGTGTTCGGAGAACAATGGCGTCTAGAGCCAATGTCATTAGAAAGAAAAACAAGATGGAGAAAAGAAATCGATTTGCTCTTATCCGTCACAGACTACATTGTTGAGTTTGTTCCTTCACAACAGAAATCCAAGGATGGAACCAATATGGAGGTAAACCTTCGATTTCGAATCTGTAGTTAGAATCGACTTGTATGATTCTCACAACCTTAGGTAAGGGAATTCATGAACACCTGGAGCTTGACTCAAATGTTCCTTCCTTTCTCATTCAATCTTACAAATGAAGTTTATGGCTGTTTTAGATAATGGTGACCCGGCAACGAAATGACTTGCACTTGAACATCCCAGCTTTGAGGAAGCTTGATGCCATGTTAATTGTAAGAACTATGCCTACAATTTACTGTATTGTTACTTGTTTTTTCTCCTTCAGTCAGAATGAATCCTATGTGATATTGGATTTGCCATGACAAAACACAGGGTTGTCTGGATAATTTTAAAGAACAGAATGAGTTCTATTATGTATCGAGAGATGCCGATGACTCGGAGAAAGGAAATAACAAGAGAAAAGATGATAAGTGGTGGCTACCTACAGCAAGAGTTCCTCCAAATGGTTTATCAGAAATGTCAAGAAAGTTTCTGCAGTACCAGAAGGATTGTGTGAACCAGGTCCTGAAGGCAGCCATGGCTATAAATGCTCAAATCATATCAGAAATGGAGATCCCTGAAGATTACATTGAATCTCTCCCTAAAGTAAGCGAAATTGAATATGTTTTGGTAATGATTTCGAAATGAATCTTCACCTGAAACTAAGTTTTGTTTTTATGGATTTACAGAATGGAAGGGCAAGCCTAGGGGATTCCATCTACAGAAGTATAACAGTTGAGTTCTTTGATCCTGATCAGTTTCTTTCCAGCATGGACTTAACATCAGATCATAAAATCCTAGATCTCAAAGACAGAATTGAAGCTTCCATAGTGATTTGGAGGAGGAAGATGAAAGATGTTAAATCTGCTTGGGCTTCAGCAGTGAGCTTGGAAAAGAGAGAACTATTTGAAGAGAGAGCAGAGACCATCTTGCTAATACTTAAGCACCGATTTCCCGGAATCCCACAATCTTCACTAGATATCAGCAAAATTCAATTCAATCGGGTATAACAATGCTTCTTCCTTGTCTAGACTCTCGATAATTATCTTTTCAATTCAAGTTTAACAAGAGAAGTAGGAGAAGCATCACAAAGAACAGAAAAGAAACGTAAACCAAACTTGTTTGAAATCTTTCAAGATCAATCAGAACTTACTTTTTCCTCCATTGCAGGACGTAGGGCACGCCATTTTGGAGAGCTACTCCAGAATACTGGAAAGCCTAGCCTTCACAGTGATGTCTCGAATCGAAGACGTACTCCACGCTGATGCCGTAACTCAAAATCCATCACAGACAGCACCGAAGAGGAAACCAGCGAGCGAAACTCCACCGGAAAAAACGCCATGCAAAAGAGAAGACAACGGCACAGAAACGCCGGTTTCAATGACACTGTTGGATTTCATGGGTTGGGGTCAGGAACAAAGCGAGAACGAGCCGAAGAAGGCTTCACTGGGGCAGTCGGATGAATTAAACGCCGATTCAGATGCGAAAAACGGAAATACGCCTGGGAATATAGTGACAAACAAGAAGGTTTCGTACGTGGAAAATTTGAGCGCTGTGAAAAGTCCAACGGCCCGCCATTGAAGAGGGATTGAAGAACAAAAAAGGTGCAGGAAGATGACAATTAGATAGAGGGATAGGGAGAATTGCAAAAGAAAGTGAATTATCAATGAATTTGGGGTTCCTTTTTTTTTTTTTTTTCTTCCCACATTTGGGATTTGACAATTTTCTATCTCATTCCTTGTGTAAATAGGAGGAAGAATGAGAAGAACAAAGAACACACTTTAATTTGGATTTTGATTTGTGTAAAACCAAAATGGCTTTTGTATAATTTGTATCATTGTATCAATCTAGTGTTATTAACCATCATAAATATCATATATTTTTCGTTA

mRNA sequence

AATAAAGGGGAAGGGAGGGAGGGGGAGAGAGAGCAAAACCAAAATCTTTTCCTTATTCCTCAGTTCAAAGGAGGTAAAGAGAAGCAAAACAAAACCATCCCACCACCATTTTTCTTGTTTTTTGTCTAAAAATCCTGCTTCTCTTCCTCCTTTTTTTTTTCTTAATTCCCTTCCTTTTCTCTCTATGCTGTCTGAAACCCAGAAGCAATTGATCACCCAGTTGCCGGTTTGTTGTCCCTTGCGTTTTCCGTCAAAAGTTTCATCAAGAAACTGAAGATGATTCATGCTATGGAGCAGGAGCACGAAGGTTTCAAATCCAAATTATGTCATTTCAAAGGAACGCACGAGAATTCTGGCAGACATGCCAAGAGCTTGAGCATCGAGGGTGCCGGCATGTTGGACTCTCTTGTCGACGAGGATACATCAAGAAGCCAAGGATCGACGCCAGTTCACAACAACTCTGCAGAGAAGCCGCTGACACCAACCAATCGACCTGAAAAGTCTCTGAAGCTGAAGTTGACCAAGGATGAAGATGCTGTCACTGAGAAGGAACCCAGAGACAAGAAGGCCTTAGAAATGGAACAAATGAAAGAGAGGTTTGCAAAGTTACTCTTGGGAGAAGACATGTCTGGGGGAGGAAAGGGTGTTTCCTCAGCCTTGGCATTGTCTAATGCAATCACAAACCTTGCTGCTTCTGTGTTCGGAGAACAATGGCGTCTAGAGCCAATGTCATTAGAAAGAAAAACAAGATGGAGAAAAGAAATCGATTTGCTCTTATCCGTCACAGACTACATTGTTGAGTTTGTTCCTTCACAACAGAAATCCAAGGATGGAACCAATATGGAGATAATGGTGACCCGGCAACGAAATGACTTGCACTTGAACATCCCAGCTTTGAGGAAGCTTGATGCCATGTTAATTGGTTGTCTGGATAATTTTAAAGAACAGAATGAGTTCTATTATGTATCGAGAGATGCCGATGACTCGGAGAAAGGAAATAACAAGAGAAAAGATGATAAGTGGTGGCTACCTACAGCAAGAGTTCCTCCAAATGGTTTATCAGAAATGTCAAGAAAGTTTCTGCAGTACCAGAAGGATTGTGTGAACCAGGTCCTGAAGGCAGCCATGGCTATAAATGCTCAAATCATATCAGAAATGGAGATCCCTGAAGATTACATTGAATCTCTCCCTAAAAATGGAAGGGCAAGCCTAGGGGATTCCATCTACAGAAGTATAACAGTTGAGTTCTTTGATCCTGATCAGTTTCTTTCCAGCATGGACTTAACATCAGATCATAAAATCCTAGATCTCAAAGACAGAATTGAAGCTTCCATAGTGATTTGGAGGAGGAAGATGAAAGATGTTAAATCTGCTTGGGCTTCAGCAGTGAGCTTGGAAAAGAGAGAACTATTTGAAGAGAGAGCAGAGACCATCTTGCTAATACTTAAGCACCGATTTCCCGGAATCCCACAATCTTCACTAGATATCAGCAAAATTCAATTCAATCGGGACGTAGGGCACGCCATTTTGGAGAGCTACTCCAGAATACTGGAAAGCCTAGCCTTCACAGTGATGTCTCGAATCGAAGACGTACTCCACGCTGATGCCGTAACTCAAAATCCATCACAGACAGCACCGAAGAGGAAACCAGCGAGCGAAACTCCACCGGAAAAAACGCCATGCAAAAGAGAAGACAACGGCACAGAAACGCCGGTTTCAATGACACTGTTGGATTTCATGGGTTGGGGTCAGGAACAAAGCGAGAACGAGCCGAAGAAGGCTTCACTGGGGCAGTCGGATGAATTAAACGCCGATTCAGATGCGAAAAACGGAAATACGCCTGGGAATATAGTGACAAACAAGAAGGTTTCGTACGTGGAAAATTTGAGCGCTGTGAAAAGTCCAACGGCCCGCCATTGAAGAGGGATTGAAGAACAAAAAAGGTGCAGGAAGATGACAATTAGATAGAGGGATAGGGAGAATTGCAAAAGAAAGTGAATTATCAATGAATTTGGGGTTCCTTTTTTTTTTTTTTTTCTTCCCACATTTGGGATTTGACAATTTTCTATCTCATTCCTTGTGTAAATAGGAGGAAGAATGAGAAGAACAAAGAACACACTTTAATTTGGATTTTGATTTGTGTAAAACCAAAATGGCTTTTGTATAATTTGTATCATTGTATCAATCTAGTGTTATTAACCATCATAAATATCATATATTTTTCGTTA

Coding sequence (CDS)

ATGATTCATGCTATGGAGCAGGAGCACGAAGGTTTCAAATCCAAATTATGTCATTTCAAAGGAACGCACGAGAATTCTGGCAGACATGCCAAGAGCTTGAGCATCGAGGGTGCCGGCATGTTGGACTCTCTTGTCGACGAGGATACATCAAGAAGCCAAGGATCGACGCCAGTTCACAACAACTCTGCAGAGAAGCCGCTGACACCAACCAATCGACCTGAAAAGTCTCTGAAGCTGAAGTTGACCAAGGATGAAGATGCTGTCACTGAGAAGGAACCCAGAGACAAGAAGGCCTTAGAAATGGAACAAATGAAAGAGAGGTTTGCAAAGTTACTCTTGGGAGAAGACATGTCTGGGGGAGGAAAGGGTGTTTCCTCAGCCTTGGCATTGTCTAATGCAATCACAAACCTTGCTGCTTCTGTGTTCGGAGAACAATGGCGTCTAGAGCCAATGTCATTAGAAAGAAAAACAAGATGGAGAAAAGAAATCGATTTGCTCTTATCCGTCACAGACTACATTGTTGAGTTTGTTCCTTCACAACAGAAATCCAAGGATGGAACCAATATGGAGATAATGGTGACCCGGCAACGAAATGACTTGCACTTGAACATCCCAGCTTTGAGGAAGCTTGATGCCATGTTAATTGGTTGTCTGGATAATTTTAAAGAACAGAATGAGTTCTATTATGTATCGAGAGATGCCGATGACTCGGAGAAAGGAAATAACAAGAGAAAAGATGATAAGTGGTGGCTACCTACAGCAAGAGTTCCTCCAAATGGTTTATCAGAAATGTCAAGAAAGTTTCTGCAGTACCAGAAGGATTGTGTGAACCAGGTCCTGAAGGCAGCCATGGCTATAAATGCTCAAATCATATCAGAAATGGAGATCCCTGAAGATTACATTGAATCTCTCCCTAAAAATGGAAGGGCAAGCCTAGGGGATTCCATCTACAGAAGTATAACAGTTGAGTTCTTTGATCCTGATCAGTTTCTTTCCAGCATGGACTTAACATCAGATCATAAAATCCTAGATCTCAAAGACAGAATTGAAGCTTCCATAGTGATTTGGAGGAGGAAGATGAAAGATGTTAAATCTGCTTGGGCTTCAGCAGTGAGCTTGGAAAAGAGAGAACTATTTGAAGAGAGAGCAGAGACCATCTTGCTAATACTTAAGCACCGATTTCCCGGAATCCCACAATCTTCACTAGATATCAGCAAAATTCAATTCAATCGGGACGTAGGGCACGCCATTTTGGAGAGCTACTCCAGAATACTGGAAAGCCTAGCCTTCACAGTGATGTCTCGAATCGAAGACGTACTCCACGCTGATGCCGTAACTCAAAATCCATCACAGACAGCACCGAAGAGGAAACCAGCGAGCGAAACTCCACCGGAAAAAACGCCATGCAAAAGAGAAGACAACGGCACAGAAACGCCGGTTTCAATGACACTGTTGGATTTCATGGGTTGGGGTCAGGAACAAAGCGAGAACGAGCCGAAGAAGGCTTCACTGGGGCAGTCGGATGAATTAAACGCCGATTCAGATGCGAAAAACGGAAATACGCCTGGGAATATAGTGACAAACAAGAAGGTTTCGTACGTGGAAAATTTGAGCGCTGTGAAAAGTCCAACGGCCCGCCATTGA

Protein sequence

MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHNNSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKMKDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILESYSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPVSMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAVKSPTARH
Homology
BLAST of CmaCh08G007050 vs. ExPASy Swiss-Prot
Match: Q9CA89 (Rop guanine nucleotide exchange factor 12 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF12 PE=1 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 4.3e-167
Identity = 340/556 (61.15%), Postives = 408/556 (73.38%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFK---GTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTP 60
           M+ A EQE E ++S+L +FK     + ++ RH KSLS+E            T   + +T 
Sbjct: 1   MVRASEQEQETYRSRLFNFKWRNNDNNSATRHNKSLSVE------------TGLDEAATG 60

Query: 61  VHNNSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKAL-EMEQMKERFAKLLLGED 120
            H+      + P+  P  S          A+   + R+++ L +MEQMKERF+KLLLGED
Sbjct: 61  SHDAEPLTIIHPSQGPPLSRSAADEAVLAALAASQARERQLLADMEQMKERFSKLLLGED 120

Query: 121 MSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEF 180
            SGGGKGVSSALALSNAITNLAASVFGEQ RLEPM  ER+ RWRKEID LLSVTDY+VEF
Sbjct: 121 NSGGGKGVSSALALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLSVTDYVVEF 180

Query: 181 VPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADD 240
            PSQQK+KDGTNMEIM TRQR DLH+NIPAL+KLDAMLI CL+NFK+Q+EF Y+S+D+ D
Sbjct: 181 APSQQKNKDGTNMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFSYISKDSPD 240

Query: 241 SEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEI 300
            +    KR D+KWW+PT +VPP+GLSE SR+FLQYQKDCVNQVLKAAMAINAQ++ EMEI
Sbjct: 241 LD---GKRNDEKWWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINAQVLFEMEI 300

Query: 301 PEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIW 360
           PE YI+SLPKNGRASLGD +Y++ITV+FFDPDQFLSSMD++S+HKI+DLK+RIEASI+IW
Sbjct: 301 PESYIDSLPKNGRASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNRIEASIIIW 360

Query: 361 RRKM--KDVKSA--WASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRD 420
           +RKM  KD KS+  WAS VSLEKRE+FEERAETILLILK R+PGI QSSLDISKIQFN D
Sbjct: 361 KRKMVYKDNKSSAPWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDISKIQFNED 420

Query: 421 VGHAILESYSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKRE 480
           VG A+LESYSRILESLA+TV+SRI+DVL AD       +   KR          TP + E
Sbjct: 421 VGQAVLESYSRILESLAYTVLSRIDDVLEAD-------RAGNKR---------NTPLEAE 480

Query: 481 DNGTETPV-SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKV 540
           +   ET V SMTL DFMGW  +Q+ N    A L    +L  D   K      ++VT KK 
Sbjct: 481 E---ETLVGSMTLSDFMGWDFDQAAN----AELESKKDLPDDPLIKE---KLSVVTTKKT 515

Query: 541 SYVENLSAVKSPTARH 548
           SY+E L  VKSPTARH
Sbjct: 541 SYLETLGGVKSPTARH 515

BLAST of CmaCh08G007050 vs. ExPASy Swiss-Prot
Match: Q9M811 (Rop guanine nucleotide exchange factor 11 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF11 PE=1 SV=2)

HSP 1 Score: 586.6 bits (1511), Expect = 2.8e-166
Identity = 343/556 (61.69%), Postives = 402/556 (72.30%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENS-GRHAKSLSIEGAGML---DSLVDEDTSRSQ-GS 60
           M+ AMEQE E +KS+L HFK  +ENS  RH KS S + A  +   D+L D+D       S
Sbjct: 1   MVKAMEQEQETYKSRLFHFKNMNENSASRHVKSWSSDCAMRMDGSDNLDDDDNDMMMFRS 60

Query: 61  TPVHNNSAEKPLTPTN--RPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLL 120
            P    S ++P  P     P ++ KL      D++   E        MEQMKE+F+KLLL
Sbjct: 61  QPGKCGSVDRPSLPIGGVTPNRNDKLPRVSSSDSM---EALIILQAAMEQMKEKFSKLLL 120

Query: 121 GEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYI 180
           GEDMSGGGKGVSSALALSNAITNLAAS FGEQ RLEPM+++RKTRWR+EI  L+SV DYI
Sbjct: 121 GEDMSGGGKGVSSALALSNAITNLAASAFGEQRRLEPMAVDRKTRWRREIGWLISVADYI 180

Query: 181 VEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRD 240
           VEF P+QQ +KDGT+ME+M TRQR DL  NIPAL+KLDAML+ CLD FK+Q+EFYYV +D
Sbjct: 181 VEFAPTQQTNKDGTSMEVMSTRQRTDLLCNIPALKKLDAMLLDCLDKFKDQDEFYYVKKD 240

Query: 241 ADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISE 300
           + DS      R D+KWWLP  +VPPNGLSE+SR+FLQ QK+CVNQVLKAAMAINAQ++SE
Sbjct: 241 SPDS---CETRNDEKWWLPAVKVPPNGLSEISRRFLQSQKECVNQVLKAAMAINAQVLSE 300

Query: 301 MEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASI 360
           MEIPE Y+ESLPKNGRASLGD IYR ITVE FD DQFL  MDL+S+HKILDLK+RIEASI
Sbjct: 301 MEIPESYLESLPKNGRASLGDVIYRMITVEMFDADQFLIEMDLSSEHKILDLKNRIEASI 360

Query: 361 VIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNR 420
           VIW+RKM  KD KS W S VS+EKRE FEERAETILL+LK  FPGI QSSLDISKIQFNR
Sbjct: 361 VIWKRKMVQKDTKSPWGSTVSIEKREQFEERAETILLLLKQGFPGISQSSLDISKIQFNR 420

Query: 421 DVGHAILESYSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKR 480
           DVG AILESYSR+LESLA TVMSRIEDVL+AD +TQ P+  AP +   S    EK   +R
Sbjct: 421 DVGLAILESYSRVLESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSLKENEKLREER 480

Query: 481 EDNGTETPVSMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKV 540
             + TE   S TL D M WG +   NE KK S     E    S        G +  NKK 
Sbjct: 481 L-SFTEDMASGTLSDVMQWGNK--NNEMKKESFFGDREKPLLS-----KVTGIMTNNKKS 540

Query: 541 SYVENLSAVKSPTARH 548
           SY++NL A++SPTAR+
Sbjct: 541 SYLDNLGAMRSPTARY 542

BLAST of CmaCh08G007050 vs. ExPASy Swiss-Prot
Match: Q9LV40 (Rho guanine nucleotide exchange factor 8 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF8 PE=1 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.5e-151
Identity = 307/548 (56.02%), Postives = 379/548 (69.16%), Query Frame = 0

Query: 14  SKLCHFKGTHENSG-RHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHNNSAEKPL---TP 73
           SK  +FK   ++S  +  +S +I        +V+ +T  SQ S     +  E  L   +P
Sbjct: 12  SKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESNTPESQNSDSFVESPVESSLPMISP 71

Query: 74  TNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGGGKGVSSALA 133
             RP K                   +++  +ME MK+RFAKLLLGEDMSGGGKGVSSALA
Sbjct: 72  LTRPGKR-----------------SERQQADMEMMKDRFAKLLLGEDMSGGGKGVSSALA 131

Query: 134 LSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNM 193
           LSNAITNLAAS+FGEQ +L+PM  +R+ RW+KEID LLSVTD+IVEFVPSQQ SKDG   
Sbjct: 132 LSNAITNLAASIFGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSKDGVCT 191

Query: 194 EIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKGNNKRKDDKW 253
           EIMVTRQR DL +NIPALRKLDAMLI  LDNF+  NEF+YVSRD+++ ++  N R +DKW
Sbjct: 192 EIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDRTNDKW 251

Query: 254 WLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGR 313
           WLP  +VPP GLSE SR+ L +QKD V QV KAAMAINAQ++SEMEIPE YI+SLPKNGR
Sbjct: 252 WLPPVKVPPGGLSEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSLPKNGR 311

Query: 314 ASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM--KDVKSAW 373
           ASLGDSIY+SIT E+FDP+QFL+ +D++++HK+LDLK+RIEAS+VIW+RK+  KD KS+W
Sbjct: 312 ASLGDSIYKSITEEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKDTKSSW 371

Query: 374 ASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILESYSRILES 433
            SAVSLEKRELFEERAETIL++LK +FPG+PQSSLDISKIQFN+DVG A+LESYSRILES
Sbjct: 372 GSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYSRILES 431

Query: 434 LAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTE-----TPVSM 493
           LA+TVMSRIEDVL+ D +    +  A +      T    +      N  E      P S 
Sbjct: 432 LAYTVMSRIEDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSK 491

Query: 494 TLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSY---VENLSA 548
           TLLDFMGW    S+                D   K+ N     +T KK+SY   +ENL+ 
Sbjct: 492 TLLDFMGWNDNSSK--------------GGDKPTKSPN-----LTPKKLSYLEKLENLNG 523

BLAST of CmaCh08G007050 vs. ExPASy Swiss-Prot
Match: Q9SVQ3 (Rop guanine nucleotide exchange factor 9 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF9 PE=1 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.8e-147
Identity = 298/531 (56.12%), Postives = 380/531 (71.56%), Query Frame = 0

Query: 31  KSLSIEGAGMLDSLVDEDTSRSQGSTPVHNNSAEKPLTPTNRPEKSLKLKLTKDEDAVTE 90
           + +SI  +  LD + D    + Q     H      P + T        ++ ++   +   
Sbjct: 7   RGISITSSFNLDRMFDSSPGKEQQQP--HLAETTMPESQTQDSLGGSPVETSRPMTSRLI 66

Query: 91  KEPRDKKALEMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEP 150
              +DK+  E E MK+RF KLLLGEDMSGGGKGVSSALALSNAITNLAAS+FGEQ +L+P
Sbjct: 67  SRRQDKQQSETEMMKDRFTKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGEQTKLQP 126

Query: 151 MSLERKTRWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKL 210
           M+ +R+ RW+KEID LLSVTD+IVEFVPSQQ SK+G   EIMVTRQR DL +NIPALRKL
Sbjct: 127 MAPDRRARWKKEIDWLLSVTDHIVEFVPSQQISKEGVCTEIMVTRQRGDLLMNIPALRKL 186

Query: 211 DAMLIGCLDNFKEQNEFYYVSRDADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQ 270
           DAMLI  LDNF+  NEF+YVSRD+++ ++  N+R  DKWWLP  +VPPNGLSE +R+ L 
Sbjct: 187 DAMLIDTLDNFRGHNEFWYVSRDSEEGKQARNERTKDKWWLPPVKVPPNGLSESARRMLH 246

Query: 271 YQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQF 330
           +QKD V+QV KAAMAINAQ++SEM IP+ YIESLPKNGR SLGDS+Y+SIT E+FDP+QF
Sbjct: 247 FQKDSVSQVQKAAMAINAQVLSEMAIPDSYIESLPKNGRVSLGDSLYKSITEEWFDPEQF 306

Query: 331 LSSMDLTSDHKILDLKDRIEASIVIWRRK--MKDVKSAWASAVSLEKRELFEERAETILL 390
           LS++DL+++HK+LD+K+RIEASIVIW+RK  +KD KS+W SAVSLEKRELFEERAETIL+
Sbjct: 307 LSTLDLSTEHKVLDVKNRIEASIVIWKRKLHLKDNKSSWGSAVSLEKRELFEERAETILV 366

Query: 391 ILKHRFPGIPQSSLDISKIQFNRDVGHAILESYSRILESLAFTVMSRIEDVLHADAVTQN 450
           +LK +FPG+PQSSLDISKIQ+N+DVGHA+LESYSRILESL +T MSRI+DVL+AD++ + 
Sbjct: 367 LLKQKFPGLPQSSLDISKIQYNKDVGHAVLESYSRILESLGYTEMSRIDDVLYADSLARK 426

Query: 451 P---SQTAPKRKPASETPPEKTPCKR------EDNGTETPVSMTLLDFMGWGQEQSENEP 510
                +T+   K A+ET               E   ++     TLLDF+GW    S+N  
Sbjct: 427 QCTGEETSDGGKIATETDSASAGSSNYSGEEIEKLESQNSSKTTLLDFIGW----SDNSS 486

Query: 511 KKASLGQSDELNADSDAKNGNTPGNIVTNKKVSY---VENLSAVKSPTARH 548
           K    GQS+        K   +P   +T KK+SY   +ENL+  +SP  RH
Sbjct: 487 K----GQSE--------KPPKSPR--MTPKKLSYLEKLENLNGFRSPKDRH 517

BLAST of CmaCh08G007050 vs. ExPASy Swiss-Prot
Match: F4J1G1 (Rop guanine nucleotide exchange factor 13 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF13 PE=1 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 3.4e-135
Identity = 291/589 (49.41%), Postives = 384/589 (65.20%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHE--NSGRHAKSLSIEGAGML-DSLVDEDTSRSQGSTP 60
           M+ A E+EHE +K KL   +   +  +S RH K  + + A  + D  +D+ T   + +T 
Sbjct: 1   MVKASEKEHEKYKPKLFDLENVKKKNSSSRHFKRWNSDSALRIEDPDIDDGTVFKKTAT- 60

Query: 61  VHNNSAEKPLTPTNRPEKSLKLKLTKDEDA--------------VTEKEPRDKKAL---- 120
               S+ +P+ P    ++  + +   DE++                EK  RD+  L    
Sbjct: 61  ----SSIQPILPVLAMDEQPQPREATDEESQKDSGKREIISFLGYNEKFTRDQVILRFRG 120

Query: 121 ------------EMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWR 180
                       E+EQMK++FAKLLLGEDMSGG KGVSSALALSNAITNLAAS FGE  R
Sbjct: 121 LQAKSCYFAYVTELEQMKDKFAKLLLGEDMSGGSKGVSSALALSNAITNLAASAFGEIRR 180

Query: 181 LEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPAL 240
           LE +S ++K RWR+EI  LLSVTD+IVEF P+ Q ++DG++ME+M T+QR DL  NIP+L
Sbjct: 181 LEAISEDKKERWRREIGWLLSVTDHIVEFSPTHQTNEDGSSMEVMTTKQRTDLVSNIPSL 240

Query: 241 RKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRK 300
           +KLD ML+ CLD FK+Q+EFYYV+  + +SE  N+ R DDKWWLP  +VPP GLSE  ++
Sbjct: 241 KKLDEMLLDCLDKFKDQDEFYYVTPGSPESENSNSTRNDDKWWLPIVKVPPKGLSETLKR 300

Query: 301 FLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDP 360
           FL  Q++CV QVL +AMAIN+Q+++EMEIPE YI+SLPK GRASLGD IYR IT+E FD 
Sbjct: 301 FLLSQRECVCQVLNSAMAINSQVLTEMEIPESYIDSLPKKGRASLGDMIYRMITLEMFDA 360

Query: 361 DQFLSSMDLTSDHKILDLKDRIEASIVIWRRKMKDV----KSAWASAVSLEKRELFEERA 420
           +QFL  MDL+S+HKILDLK++ EAS+VIW+RK+  +     S W++ +S++KR+  EERA
Sbjct: 361 EQFLLEMDLSSEHKILDLKNKFEASVVIWQRKIVQIDNKSSSPWSTNLSMDKRQQLEERA 420

Query: 421 ETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILESYSRILESLAFTVMSRIEDVLHAD 480
            TIL ++K  FPGI QS+LDISKIQFNRD+G AI+ESYSRILESLA TVMSRIEDVL AD
Sbjct: 421 ATILQLIKQEFPGISQSTLDISKIQFNRDIGLAIVESYSRILESLAHTVMSRIEDVLEAD 480

Query: 481 AVTQNPSQTAPKRKPASET-PPEKTP----CKREDNGTETPVSMTLLDFMGWGQEQSENE 540
            +TQNP     K     ET  PEK      C  ED   +   +++L + M W  E   NE
Sbjct: 481 QLTQNPELAMCKIHIVKETESPEKEEEPNFCLLEDRPKKQKPTISLSEVMQWNIE--TNE 540

Query: 541 PKKASLGQSDELNADSDAKNGNTPGNIV--TNKKVSYVENLSAVKSPTA 546
           P+K            SD K      +++   NKK +Y+E+L   +SPTA
Sbjct: 541 PRKEK----------SDKKLLTRVSSMIMSNNKKTTYLESLGTTRSPTA 572

BLAST of CmaCh08G007050 vs. ExPASy TrEMBL
Match: A0A6J1KI31 (rop guanine nucleotide exchange factor 12-like OS=Cucurbita maxima OX=3661 GN=LOC111494607 PE=4 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 1.3e-307
Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN 60
           MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN 60

Query: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120
           NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG
Sbjct: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120

Query: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180
           GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ
Sbjct: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180

Query: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240
           QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG
Sbjct: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240

Query: 241 NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300
           NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY
Sbjct: 241 NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300

Query: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360
           IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM
Sbjct: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360

Query: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420
           KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES
Sbjct: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420

Query: 421 YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV 480
           YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV
Sbjct: 421 YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV 480

Query: 481 SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540
           SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV
Sbjct: 481 SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540

Query: 541 KSPTARH 548
           KSPTARH
Sbjct: 541 KSPTARH 547

BLAST of CmaCh08G007050 vs. ExPASy TrEMBL
Match: A0A6J1HL16 (rop guanine nucleotide exchange factor 12-like OS=Cucurbita moschata OX=3662 GN=LOC111464520 PE=4 SV=1)

HSP 1 Score: 1048.5 bits (2710), Expect = 9.7e-303
Identity = 539/547 (98.54%), Postives = 543/547 (99.27%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN 60
           MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIE A MLDSLVDEDTSRSQGSTPVHN
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIESASMLDSLVDEDTSRSQGSTPVHN 60

Query: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120
           NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG
Sbjct: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120

Query: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180
           GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ
Sbjct: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180

Query: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240
           QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG
Sbjct: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240

Query: 241 NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300
           NNKRKDDKWWLPTARVPPNGLSEM+RKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY
Sbjct: 241 NNKRKDDKWWLPTARVPPNGLSEMTRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300

Query: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360
           IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM
Sbjct: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360

Query: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420
           KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES
Sbjct: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420

Query: 421 YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV 480
           YSRILESLAFTVMSRIEDVLHADA+TQNPSQTA KRKPASETPPEKTP KREDNGTETPV
Sbjct: 421 YSRILESLAFTVMSRIEDVLHADALTQNPSQTASKRKPASETPPEKTPRKREDNGTETPV 480

Query: 481 SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540
           SMTLLDFMGWGQEQSENEPKK+SLGQSDELNADSDAKNGNTPGN+VTNKKVSYVENLSAV
Sbjct: 481 SMTLLDFMGWGQEQSENEPKKSSLGQSDELNADSDAKNGNTPGNVVTNKKVSYVENLSAV 540

Query: 541 KSPTARH 548
           KSPTARH
Sbjct: 541 KSPTARH 547

BLAST of CmaCh08G007050 vs. ExPASy TrEMBL
Match: A0A5D3C0X6 (Rop guanine nucleotide exchange factor 12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002180 PE=4 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 5.2e-256
Identity = 471/557 (84.56%), Postives = 503/557 (90.31%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDED--TSRSQGSTPV 60
           M+ AMEQEHEGF+S+   F+GTHEN+GRHAKSLS+E A + DS  ++D  TSRSQGSTPV
Sbjct: 1   MVRAMEQEHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTPV 60

Query: 61  HNNSAEK-----PLTPTNRPEKSLKLKL-TKDEDAVTEKEPRDKKALEMEQMKERFAKLL 120
           HNNS+EK     PL P+NR E++ K KL +KD+D  TEKE R+K ALEMEQMKERFAKLL
Sbjct: 61  HNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERK RWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSR 240
           IVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLI CLDNFK+QNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240

Query: 241 DADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADDSEKGNNKRKDDKWWLPTA+VPPNGLS+MSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTS+HKILDLKDRIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 360

Query: 361 IVIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKM  KD KS W SAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAILESYSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCK 480
           RDVGHA+LESYSRILESLAFTVMSRIEDVLHAD +TQNPSQ A +RKP SE P EK+  +
Sbjct: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKSE-E 480

Query: 481 REDNGTETPVSMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKK 540
             +NG ETP SMTLLDFMGWGQ+Q+E E KK   G SD+LNADSD K GN PGNIVTNKK
Sbjct: 481 LNNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNKK 540

Query: 541 VSYVENLSAVKSPTARH 548
           VSY+ENLSAV+SPTARH
Sbjct: 541 VSYLENLSAVRSPTARH 556

BLAST of CmaCh08G007050 vs. ExPASy TrEMBL
Match: A0A1S3CGA0 (LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like OS=Cucumis melo OX=3656 GN=LOC103500592 PE=4 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 5.7e-255
Identity = 469/557 (84.20%), Postives = 502/557 (90.13%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDED--TSRSQGSTPV 60
           M+ AMEQEHEGF+S+   F+GTHEN+GRHAKSLS+E A + DS  ++D  TSRSQGSTPV
Sbjct: 1   MVRAMEQEHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTPV 60

Query: 61  HNNSAEK-----PLTPTNRPEKSLKLKL-TKDEDAVTEKEPRDKKALEMEQMKERFAKLL 120
           HNNS+EK     PL P+NR E++ K KL +KD+D  TEKE R+K ALEMEQMKERFAKLL
Sbjct: 61  HNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKLL 120

Query: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDY 180
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERK RWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTDY 180

Query: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSR 240
           IVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLI CLDNFK+QNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 240

Query: 241 DADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300
           DADDSEKGNNK KDDKWWLPTA+VPPNGLS+MSRKFLQYQKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKXKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEAS 360
           EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTS+HKILDLKDRIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 360

Query: 361 IVIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420
           IVIWRRKM  KD KS W SAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 421 RDVGHAILESYSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCK 480
           RDVGHA+LESYSRILESLAFTVMSRIEDVLHAD +TQNPSQ A +RKP SE P EK+  +
Sbjct: 421 RDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKSE-E 480

Query: 481 REDNGTETPVSMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKK 540
             +NG ETP SMTLLDFMGWGQ+Q+E E KK   G SD+LNADSD K GN PGNIVTNK+
Sbjct: 481 LNNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNKR 540

Query: 541 VSYVENLSAVKSPTARH 548
           VSY+ENLSAV+SPTARH
Sbjct: 541 VSYLENLSAVRSPTARH 556

BLAST of CmaCh08G007050 vs. ExPASy TrEMBL
Match: A0A0A0K7D8 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G446980 PE=4 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.3e-250
Identity = 462/556 (83.09%), Postives = 497/556 (89.39%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDED--TSRSQGSTPV 60
           M+ AMEQEHEGF+S+   F+G HEN+GRHAKSLS+E A + DS+ ++D  TSRSQGSTPV
Sbjct: 1   MVRAMEQEHEGFRSRFFLFRGNHENTGRHAKSLSVESANVFDSVNEDDLSTSRSQGSTPV 60

Query: 61  HNNS----AEKPLTPTNRPEKSLKLKL-TKDEDAVTEKEPRDKKALEMEQMKERFAKLLL 120
           HN+S    A  PL P+NR E++ K KL +KD D   EKE R+K ALEMEQMKERFAKLLL
Sbjct: 61  HNSSEKHQAGPPLAPSNRSERASKPKLSSKDNDLAAEKEAREKMALEMEQMKERFAKLLL 120

Query: 121 GEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYI 180
           GEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS+ERK RWRKEIDLLLSVTDYI
Sbjct: 121 GEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTDYI 180

Query: 181 VEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRD 240
           VEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLI CLDNFK+QNEFYYVSRD
Sbjct: 181 VEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSRD 240

Query: 241 ADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISE 300
           A+DSEKGNNKR +DKWWLPTA+VPPNGLS+MSRKFLQYQKDCVNQVLKAAMAINAQIISE
Sbjct: 241 ANDSEKGNNKR-NDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQIISE 300

Query: 301 MEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASI 360
           MEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTS+HKILDLKDRIEASI
Sbjct: 301 MEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEASI 360

Query: 361 VIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNR 420
           VIWRRKM  KD KS W SAVS+EKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNR
Sbjct: 361 VIWRRKMNQKDGKSTWGSAVSMEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNR 420

Query: 421 DVGHAILESYSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKR 480
           DVGHA+LESYSRILESLAFTVMSRIEDVLHAD +TQNPSQ A +RKP SE P EK   + 
Sbjct: 421 DVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEK--LEE 480

Query: 481 EDNGTETPVSMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKV 540
            +NG ETP SMTLLDFMGWGQ+Q+E E KK S G SD+LN DSD K GN  GNIVTNKKV
Sbjct: 481 LNNGPETPASMTLLDFMGWGQDQNETEMKKESFGNSDDLNLDSDLKQGNKAGNIVTNKKV 540

Query: 541 SYVENLSAVKSPTARH 548
           SY+ENLSAV+SPTARH
Sbjct: 541 SYLENLSAVRSPTARH 553

BLAST of CmaCh08G007050 vs. NCBI nr
Match: XP_023000350.1 (rop guanine nucleotide exchange factor 12-like [Cucurbita maxima])

HSP 1 Score: 1064.7 bits (2752), Expect = 2.7e-307
Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN 60
           MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN 60

Query: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120
           NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG
Sbjct: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120

Query: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180
           GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ
Sbjct: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180

Query: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240
           QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG
Sbjct: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240

Query: 241 NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300
           NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY
Sbjct: 241 NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300

Query: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360
           IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM
Sbjct: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360

Query: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420
           KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES
Sbjct: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420

Query: 421 YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV 480
           YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV
Sbjct: 421 YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV 480

Query: 481 SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540
           SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV
Sbjct: 481 SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540

Query: 541 KSPTARH 548
           KSPTARH
Sbjct: 541 KSPTARH 547

BLAST of CmaCh08G007050 vs. NCBI nr
Match: XP_023514662.1 (rop guanine nucleotide exchange factor 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1052.0 bits (2719), Expect = 1.8e-303
Identity = 541/547 (98.90%), Postives = 544/547 (99.45%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN 60
           MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIE A MLDSLVDEDTSRSQGSTPVHN
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIESASMLDSLVDEDTSRSQGSTPVHN 60

Query: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120
           NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG
Sbjct: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120

Query: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180
           GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ
Sbjct: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180

Query: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240
           QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG
Sbjct: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240

Query: 241 NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300
           NNKRKDDKWWLPTARVPPNGLSEM+RKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY
Sbjct: 241 NNKRKDDKWWLPTARVPPNGLSEMTRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300

Query: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360
           IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM
Sbjct: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360

Query: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420
           KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES
Sbjct: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420

Query: 421 YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV 480
           YSRILESLAFTVMSRIEDVLHADA+TQNPSQTAPKRKPASETPPEKTP KREDNGTETPV
Sbjct: 421 YSRILESLAFTVMSRIEDVLHADALTQNPSQTAPKRKPASETPPEKTPRKREDNGTETPV 480

Query: 481 SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540
           SMTLLDFMGWGQEQSENEPKK+SLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV
Sbjct: 481 SMTLLDFMGWGQEQSENEPKKSSLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540

Query: 541 KSPTARH 548
           KSPTARH
Sbjct: 541 KSPTARH 547

BLAST of CmaCh08G007050 vs. NCBI nr
Match: KAG6593466.1 (Rop guanine nucleotide exchange factor 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.5e-302
Identity = 540/547 (98.72%), Postives = 543/547 (99.27%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN 60
           MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIE A MLDSLVDEDTSRSQGSTPVHN
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIESASMLDSLVDEDTSRSQGSTPVHN 60

Query: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120
           NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG
Sbjct: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120

Query: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180
           GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ
Sbjct: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180

Query: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240
           QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG
Sbjct: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240

Query: 241 NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300
           NNKRKDDKWWLPTARVPPNGLSEM+RKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY
Sbjct: 241 NNKRKDDKWWLPTARVPPNGLSEMTRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300

Query: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360
           IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM
Sbjct: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360

Query: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420
           KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES
Sbjct: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420

Query: 421 YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV 480
           YSRILESLAFTVMSRIEDVLHADA+TQNPSQTA KRKPASETPPEKTP KREDNGTETPV
Sbjct: 421 YSRILESLAFTVMSRIEDVLHADALTQNPSQTASKRKPASETPPEKTPRKREDNGTETPV 480

Query: 481 SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540
           SMTLLDFMGWGQEQSENEPKK+SLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV
Sbjct: 481 SMTLLDFMGWGQEQSENEPKKSSLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540

Query: 541 KSPTARH 548
           KSPTARH
Sbjct: 541 KSPTARH 547

BLAST of CmaCh08G007050 vs. NCBI nr
Match: XP_022964520.1 (rop guanine nucleotide exchange factor 12-like [Cucurbita moschata])

HSP 1 Score: 1048.5 bits (2710), Expect = 2.0e-302
Identity = 539/547 (98.54%), Postives = 543/547 (99.27%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN 60
           MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIE A MLDSLVDEDTSRSQGSTPVHN
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIESASMLDSLVDEDTSRSQGSTPVHN 60

Query: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120
           NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG
Sbjct: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120

Query: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180
           GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ
Sbjct: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180

Query: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240
           QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG
Sbjct: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240

Query: 241 NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300
           NNKRKDDKWWLPTARVPPNGLSEM+RKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY
Sbjct: 241 NNKRKDDKWWLPTARVPPNGLSEMTRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300

Query: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360
           IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM
Sbjct: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360

Query: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420
           KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES
Sbjct: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420

Query: 421 YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV 480
           YSRILESLAFTVMSRIEDVLHADA+TQNPSQTA KRKPASETPPEKTP KREDNGTETPV
Sbjct: 421 YSRILESLAFTVMSRIEDVLHADALTQNPSQTASKRKPASETPPEKTPRKREDNGTETPV 480

Query: 481 SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540
           SMTLLDFMGWGQEQSENEPKK+SLGQSDELNADSDAKNGNTPGN+VTNKKVSYVENLSAV
Sbjct: 481 SMTLLDFMGWGQEQSENEPKKSSLGQSDELNADSDAKNGNTPGNVVTNKKVSYVENLSAV 540

Query: 541 KSPTARH 548
           KSPTARH
Sbjct: 541 KSPTARH 547

BLAST of CmaCh08G007050 vs. NCBI nr
Match: KAG7025807.1 (Rop guanine nucleotide exchange factor 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1047.7 bits (2708), Expect = 3.4e-302
Identity = 539/547 (98.54%), Postives = 543/547 (99.27%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHN 60
           MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIE A MLD+LVDEDTSRSQGSTPVHN
Sbjct: 1   MIHAMEQEHEGFKSKLCHFKGTHENSGRHAKSLSIESASMLDTLVDEDTSRSQGSTPVHN 60

Query: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120
           NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG
Sbjct: 61  NSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGG 120

Query: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180
           GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ
Sbjct: 121 GKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQ 180

Query: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240
           QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG
Sbjct: 181 QKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKG 240

Query: 241 NNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300
           NNKRKDDKWWLPTARVPPNGLSEM+RKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY
Sbjct: 241 NNKRKDDKWWLPTARVPPNGLSEMTRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDY 300

Query: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360
           IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM
Sbjct: 301 IESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM 360

Query: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420
           KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES
Sbjct: 361 KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILES 420

Query: 421 YSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPV 480
           YSRILESLAFTVMSRIEDVLHADA+TQNPSQTA KRKPASETPPEKTP KREDNGTETPV
Sbjct: 421 YSRILESLAFTVMSRIEDVLHADALTQNPSQTASKRKPASETPPEKTPRKREDNGTETPV 480

Query: 481 SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540
           SMTLLDFMGWGQEQSENEPKK+SLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV
Sbjct: 481 SMTLLDFMGWGQEQSENEPKKSSLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAV 540

Query: 541 KSPTARH 548
           KSPTARH
Sbjct: 541 KSPTARH 547

BLAST of CmaCh08G007050 vs. TAIR 10
Match: AT1G79860.1 (RHO guanyl-nucleotide exchange factor 12 )

HSP 1 Score: 589.3 bits (1518), Expect = 3.1e-168
Identity = 340/556 (61.15%), Postives = 408/556 (73.38%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFK---GTHENSGRHAKSLSIEGAGMLDSLVDEDTSRSQGSTP 60
           M+ A EQE E ++S+L +FK     + ++ RH KSLS+E            T   + +T 
Sbjct: 1   MVRASEQEQETYRSRLFNFKWRNNDNNSATRHNKSLSVE------------TGLDEAATG 60

Query: 61  VHNNSAEKPLTPTNRPEKSLKLKLTKDEDAVTEKEPRDKKAL-EMEQMKERFAKLLLGED 120
            H+      + P+  P  S          A+   + R+++ L +MEQMKERF+KLLLGED
Sbjct: 61  SHDAEPLTIIHPSQGPPLSRSAADEAVLAALAASQARERQLLADMEQMKERFSKLLLGED 120

Query: 121 MSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEF 180
            SGGGKGVSSALALSNAITNLAASVFGEQ RLEPM  ER+ RWRKEID LLSVTDY+VEF
Sbjct: 121 NSGGGKGVSSALALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLSVTDYVVEF 180

Query: 181 VPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADD 240
            PSQQK+KDGTNMEIM TRQR DLH+NIPAL+KLDAMLI CL+NFK+Q+EF Y+S+D+ D
Sbjct: 181 APSQQKNKDGTNMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFSYISKDSPD 240

Query: 241 SEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEI 300
            +    KR D+KWW+PT +VPP+GLSE SR+FLQYQKDCVNQVLKAAMAINAQ++ EMEI
Sbjct: 241 LD---GKRNDEKWWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINAQVLFEMEI 300

Query: 301 PEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIW 360
           PE YI+SLPKNGRASLGD +Y++ITV+FFDPDQFLSSMD++S+HKI+DLK+RIEASI+IW
Sbjct: 301 PESYIDSLPKNGRASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNRIEASIIIW 360

Query: 361 RRKM--KDVKSA--WASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRD 420
           +RKM  KD KS+  WAS VSLEKRE+FEERAETILLILK R+PGI QSSLDISKIQFN D
Sbjct: 361 KRKMVYKDNKSSAPWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDISKIQFNED 420

Query: 421 VGHAILESYSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKRE 480
           VG A+LESYSRILESLA+TV+SRI+DVL AD       +   KR          TP + E
Sbjct: 421 VGQAVLESYSRILESLAYTVLSRIDDVLEAD-------RAGNKR---------NTPLEAE 480

Query: 481 DNGTETPV-SMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKV 540
           +   ET V SMTL DFMGW  +Q+ N    A L    +L  D   K      ++VT KK 
Sbjct: 481 E---ETLVGSMTLSDFMGWDFDQAAN----AELESKKDLPDDPLIKE---KLSVVTTKKT 515

Query: 541 SYVENLSAVKSPTARH 548
           SY+E L  VKSPTARH
Sbjct: 541 SYLETLGGVKSPTARH 515

BLAST of CmaCh08G007050 vs. TAIR 10
Match: AT1G52240.1 (RHO guanyl-nucleotide exchange factor 11 )

HSP 1 Score: 561.2 bits (1445), Expect = 9.0e-160
Identity = 330/534 (61.80%), Postives = 386/534 (72.28%), Query Frame = 0

Query: 23  HENS-GRHAKSLSIEGAGML---DSLVDEDTSRSQ-GSTPVHNNSAEKPLTPTN--RPEK 82
           +ENS  RH KS S + A  +   D+L D+D       S P    S ++P  P     P +
Sbjct: 87  NENSASRHVKSWSSDCAMRMDGSDNLDDDDNDMMMFRSQPGKCGSVDRPSLPIGGVTPNR 146

Query: 83  SLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAIT 142
           + KL      D++   E        MEQMKE+F+KLLLGEDMSGGGKGVSSALALSNAIT
Sbjct: 147 NDKLPRVSSSDSM---EALIILQAAMEQMKEKFSKLLLGEDMSGGGKGVSSALALSNAIT 206

Query: 143 NLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTR 202
           NLAAS FGEQ RLEPM+++RKTRWR+EI  L+SV DYIVEF P+QQ +KDGT+ME+M TR
Sbjct: 207 NLAASAFGEQRRLEPMAVDRKTRWRREIGWLISVADYIVEFAPTQQTNKDGTSMEVMSTR 266

Query: 203 QRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKGNNKRKDDKWWLPTAR 262
           QR DL  NIPAL+KLDAML+ CLD FK+Q+EFYYV +D+ DS      R D+KWWLP  +
Sbjct: 267 QRTDLLCNIPALKKLDAMLLDCLDKFKDQDEFYYVKKDSPDS---CETRNDEKWWLPAVK 326

Query: 263 VPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDS 322
           VPPNGLSE+SR+FLQ QK+CVNQVLKAAMAINAQ++SEMEIPE Y+ESLPKNGRASLGD 
Sbjct: 327 VPPNGLSEISRRFLQSQKECVNQVLKAAMAINAQVLSEMEIPESYLESLPKNGRASLGDV 386

Query: 323 IYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM--KDVKSAWASAVSL 382
           IYR ITVE FD DQFL  MDL+S+HKILDLK+RIEASIVIW+RKM  KD KS W S VS+
Sbjct: 387 IYRMITVEMFDADQFLIEMDLSSEHKILDLKNRIEASIVIWKRKMVQKDTKSPWGSTVSI 446

Query: 383 EKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILESYSRILESLAFTVM 442
           EKRE FEERAETILL+LK  FPGI QSSLDISKIQFNRDVG AILESYSR+LESLA TVM
Sbjct: 447 EKREQFEERAETILLLLKQGFPGISQSSLDISKIQFNRDVGLAILESYSRVLESLAHTVM 506

Query: 443 SRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTETPVSMTLLDFMGWGQE 502
           SRIEDVL+AD +TQ P+  AP +   S    EK   +R  + TE   S TL D M WG +
Sbjct: 507 SRIEDVLYADQLTQEPTNNAPSKNRYSLKENEKLREERL-SFTEDMASGTLSDVMQWGNK 566

Query: 503 QSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSYVENLSAVKSPTARH 548
              NE KK S     E    S        G +  NKK SY++NL A++SPTAR+
Sbjct: 567 --NNEMKKESFFGDREKPLLS-----KVTGIMTNNKKSSYLDNLGAMRSPTARY 606

BLAST of CmaCh08G007050 vs. TAIR 10
Match: AT3G24620.1 (RHO guanyl-nucleotide exchange factor 8 )

HSP 1 Score: 537.7 bits (1384), Expect = 1.1e-152
Identity = 307/548 (56.02%), Postives = 379/548 (69.16%), Query Frame = 0

Query: 14  SKLCHFKGTHENSG-RHAKSLSIEGAGMLDSLVDEDTSRSQGSTPVHNNSAEKPL---TP 73
           SK  +FK   ++S  +  +S +I        +V+ +T  SQ S     +  E  L   +P
Sbjct: 12  SKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESNTPESQNSDSFVESPVESSLPMISP 71

Query: 74  TNRPEKSLKLKLTKDEDAVTEKEPRDKKALEMEQMKERFAKLLLGEDMSGGGKGVSSALA 133
             RP K                   +++  +ME MK+RFAKLLLGEDMSGGGKGVSSALA
Sbjct: 72  LTRPGKR-----------------SERQQADMEMMKDRFAKLLLGEDMSGGGKGVSSALA 131

Query: 134 LSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNM 193
           LSNAITNLAAS+FGEQ +L+PM  +R+ RW+KEID LLSVTD+IVEFVPSQQ SKDG   
Sbjct: 132 LSNAITNLAASIFGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSKDGVCT 191

Query: 194 EIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKGNNKRKDDKW 253
           EIMVTRQR DL +NIPALRKLDAMLI  LDNF+  NEF+YVSRD+++ ++  N R +DKW
Sbjct: 192 EIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDRTNDKW 251

Query: 254 WLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGR 313
           WLP  +VPP GLSE SR+ L +QKD V QV KAAMAINAQ++SEMEIPE YI+SLPKNGR
Sbjct: 252 WLPPVKVPPGGLSEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSLPKNGR 311

Query: 314 ASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEASIVIWRRKM--KDVKSAW 373
           ASLGDSIY+SIT E+FDP+QFL+ +D++++HK+LDLK+RIEAS+VIW+RK+  KD KS+W
Sbjct: 312 ASLGDSIYKSITEEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKDTKSSW 371

Query: 374 ASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILESYSRILES 433
            SAVSLEKRELFEERAETIL++LK +FPG+PQSSLDISKIQFN+DVG A+LESYSRILES
Sbjct: 372 GSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYSRILES 431

Query: 434 LAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCKREDNGTE-----TPVSM 493
           LA+TVMSRIEDVL+ D +    +  A +      T    +      N  E      P S 
Sbjct: 432 LAYTVMSRIEDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSK 491

Query: 494 TLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKKVSY---VENLSA 548
           TLLDFMGW    S+                D   K+ N     +T KK+SY   +ENL+ 
Sbjct: 492 TLLDFMGWNDNSSK--------------GGDKPTKSPN-----LTPKKLSYLEKLENLNG 523

BLAST of CmaCh08G007050 vs. TAIR 10
Match: AT4G13240.1 (RHO guanyl-nucleotide exchange factor 9 )

HSP 1 Score: 523.1 bits (1346), Expect = 2.7e-148
Identity = 298/531 (56.12%), Postives = 380/531 (71.56%), Query Frame = 0

Query: 31  KSLSIEGAGMLDSLVDEDTSRSQGSTPVHNNSAEKPLTPTNRPEKSLKLKLTKDEDAVTE 90
           + +SI  +  LD + D    + Q     H      P + T        ++ ++   +   
Sbjct: 7   RGISITSSFNLDRMFDSSPGKEQQQP--HLAETTMPESQTQDSLGGSPVETSRPMTSRLI 66

Query: 91  KEPRDKKALEMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEP 150
              +DK+  E E MK+RF KLLLGEDMSGGGKGVSSALALSNAITNLAAS+FGEQ +L+P
Sbjct: 67  SRRQDKQQSETEMMKDRFTKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGEQTKLQP 126

Query: 151 MSLERKTRWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKL 210
           M+ +R+ RW+KEID LLSVTD+IVEFVPSQQ SK+G   EIMVTRQR DL +NIPALRKL
Sbjct: 127 MAPDRRARWKKEIDWLLSVTDHIVEFVPSQQISKEGVCTEIMVTRQRGDLLMNIPALRKL 186

Query: 211 DAMLIGCLDNFKEQNEFYYVSRDADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQ 270
           DAMLI  LDNF+  NEF+YVSRD+++ ++  N+R  DKWWLP  +VPPNGLSE +R+ L 
Sbjct: 187 DAMLIDTLDNFRGHNEFWYVSRDSEEGKQARNERTKDKWWLPPVKVPPNGLSESARRMLH 246

Query: 271 YQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQF 330
           +QKD V+QV KAAMAINAQ++SEM IP+ YIESLPKNGR SLGDS+Y+SIT E+FDP+QF
Sbjct: 247 FQKDSVSQVQKAAMAINAQVLSEMAIPDSYIESLPKNGRVSLGDSLYKSITEEWFDPEQF 306

Query: 331 LSSMDLTSDHKILDLKDRIEASIVIWRRK--MKDVKSAWASAVSLEKRELFEERAETILL 390
           LS++DL+++HK+LD+K+RIEASIVIW+RK  +KD KS+W SAVSLEKRELFEERAETIL+
Sbjct: 307 LSTLDLSTEHKVLDVKNRIEASIVIWKRKLHLKDNKSSWGSAVSLEKRELFEERAETILV 366

Query: 391 ILKHRFPGIPQSSLDISKIQFNRDVGHAILESYSRILESLAFTVMSRIEDVLHADAVTQN 450
           +LK +FPG+PQSSLDISKIQ+N+DVGHA+LESYSRILESL +T MSRI+DVL+AD++ + 
Sbjct: 367 LLKQKFPGLPQSSLDISKIQYNKDVGHAVLESYSRILESLGYTEMSRIDDVLYADSLARK 426

Query: 451 P---SQTAPKRKPASETPPEKTPCKR------EDNGTETPVSMTLLDFMGWGQEQSENEP 510
                +T+   K A+ET               E   ++     TLLDF+GW    S+N  
Sbjct: 427 QCTGEETSDGGKIATETDSASAGSSNYSGEEIEKLESQNSSKTTLLDFIGW----SDNSS 486

Query: 511 KKASLGQSDELNADSDAKNGNTPGNIVTNKKVSY---VENLSAVKSPTARH 548
           K    GQS+        K   +P   +T KK+SY   +ENL+  +SP  RH
Sbjct: 487 K----GQSE--------KPPKSPR--MTPKKLSYLEKLENLNGFRSPKDRH 517

BLAST of CmaCh08G007050 vs. TAIR 10
Match: AT3G16130.1 (RHO guanyl-nucleotide exchange factor 13 )

HSP 1 Score: 483.4 bits (1243), Expect = 2.4e-136
Identity = 291/589 (49.41%), Postives = 384/589 (65.20%), Query Frame = 0

Query: 1   MIHAMEQEHEGFKSKLCHFKGTHE--NSGRHAKSLSIEGAGML-DSLVDEDTSRSQGSTP 60
           M+ A E+EHE +K KL   +   +  +S RH K  + + A  + D  +D+ T   + +T 
Sbjct: 1   MVKASEKEHEKYKPKLFDLENVKKKNSSSRHFKRWNSDSALRIEDPDIDDGTVFKKTAT- 60

Query: 61  VHNNSAEKPLTPTNRPEKSLKLKLTKDEDA--------------VTEKEPRDKKAL---- 120
               S+ +P+ P    ++  + +   DE++                EK  RD+  L    
Sbjct: 61  ----SSIQPILPVLAMDEQPQPREATDEESQKDSGKREIISFLGYNEKFTRDQVILRFRG 120

Query: 121 ------------EMEQMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWR 180
                       E+EQMK++FAKLLLGEDMSGG KGVSSALALSNAITNLAAS FGE  R
Sbjct: 121 LQAKSCYFAYVTELEQMKDKFAKLLLGEDMSGGSKGVSSALALSNAITNLAASAFGEIRR 180

Query: 181 LEPMSLERKTRWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPAL 240
           LE +S ++K RWR+EI  LLSVTD+IVEF P+ Q ++DG++ME+M T+QR DL  NIP+L
Sbjct: 181 LEAISEDKKERWRREIGWLLSVTDHIVEFSPTHQTNEDGSSMEVMTTKQRTDLVSNIPSL 240

Query: 241 RKLDAMLIGCLDNFKEQNEFYYVSRDADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRK 300
           +KLD ML+ CLD FK+Q+EFYYV+  + +SE  N+ R DDKWWLP  +VPP GLSE  ++
Sbjct: 241 KKLDEMLLDCLDKFKDQDEFYYVTPGSPESENSNSTRNDDKWWLPIVKVPPKGLSETLKR 300

Query: 301 FLQYQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDP 360
           FL  Q++CV QVL +AMAIN+Q+++EMEIPE YI+SLPK GRASLGD IYR IT+E FD 
Sbjct: 301 FLLSQRECVCQVLNSAMAINSQVLTEMEIPESYIDSLPKKGRASLGDMIYRMITLEMFDA 360

Query: 361 DQFLSSMDLTSDHKILDLKDRIEASIVIWRRKMKDV----KSAWASAVSLEKRELFEERA 420
           +QFL  MDL+S+HKILDLK++ EAS+VIW+RK+  +     S W++ +S++KR+  EERA
Sbjct: 361 EQFLLEMDLSSEHKILDLKNKFEASVVIWQRKIVQIDNKSSSPWSTNLSMDKRQQLEERA 420

Query: 421 ETILLILKHRFPGIPQSSLDISKIQFNRDVGHAILESYSRILESLAFTVMSRIEDVLHAD 480
            TIL ++K  FPGI QS+LDISKIQFNRD+G AI+ESYSRILESLA TVMSRIEDVL AD
Sbjct: 421 ATILQLIKQEFPGISQSTLDISKIQFNRDIGLAIVESYSRILESLAHTVMSRIEDVLEAD 480

Query: 481 AVTQNPSQTAPKRKPASET-PPEKTP----CKREDNGTETPVSMTLLDFMGWGQEQSENE 540
            +TQNP     K     ET  PEK      C  ED   +   +++L + M W  E   NE
Sbjct: 481 QLTQNPELAMCKIHIVKETESPEKEEEPNFCLLEDRPKKQKPTISLSEVMQWNIE--TNE 540

Query: 541 PKKASLGQSDELNADSDAKNGNTPGNIV--TNKKVSYVENLSAVKSPTA 546
           P+K            SD K      +++   NKK +Y+E+L   +SPTA
Sbjct: 541 PRKEK----------SDKKLLTRVSSMIMSNNKKTTYLESLGTTRSPTA 572

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9CA894.3e-16761.15Rop guanine nucleotide exchange factor 12 OS=Arabidopsis thaliana OX=3702 GN=ROP... [more]
Q9M8112.8e-16661.69Rop guanine nucleotide exchange factor 11 OS=Arabidopsis thaliana OX=3702 GN=ROP... [more]
Q9LV401.5e-15156.02Rho guanine nucleotide exchange factor 8 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9SVQ33.8e-14756.12Rop guanine nucleotide exchange factor 9 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
F4J1G13.4e-13549.41Rop guanine nucleotide exchange factor 13 OS=Arabidopsis thaliana OX=3702 GN=ROP... [more]
Match NameE-valueIdentityDescription
A0A6J1KI311.3e-307100.00rop guanine nucleotide exchange factor 12-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HL169.7e-30398.54rop guanine nucleotide exchange factor 12-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A5D3C0X65.2e-25684.56Rop guanine nucleotide exchange factor 12-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CGA05.7e-25584.20LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like OS=Cucumis m... [more]
A0A0A0K7D84.3e-25083.09PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G446980 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_023000350.12.7e-307100.00rop guanine nucleotide exchange factor 12-like [Cucurbita maxima][more]
XP_023514662.11.8e-30398.90rop guanine nucleotide exchange factor 12-like [Cucurbita pepo subsp. pepo][more]
KAG6593466.11.5e-30298.72Rop guanine nucleotide exchange factor 12, partial [Cucurbita argyrosperma subsp... [more]
XP_022964520.12.0e-30298.54rop guanine nucleotide exchange factor 12-like [Cucurbita moschata][more]
KAG7025807.13.4e-30298.54Rop guanine nucleotide exchange factor 12 [Cucurbita argyrosperma subsp. argyros... [more]
Match NameE-valueIdentityDescription
AT1G79860.13.1e-16861.15RHO guanyl-nucleotide exchange factor 12 [more]
AT1G52240.19.0e-16061.80RHO guanyl-nucleotide exchange factor 11 [more]
AT3G24620.11.1e-15256.02RHO guanyl-nucleotide exchange factor 8 [more]
AT4G13240.12.7e-14856.12RHO guanyl-nucleotide exchange factor 9 [more]
AT3G16130.12.4e-13649.41RHO guanyl-nucleotide exchange factor 13 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.58.1310PRONE domain, subdomain 2coord: 295..409
e-value: 3.5E-147
score: 491.5
NoneNo IPR availableGENE3D1.20.58.2010PRONE domain, subdomain 1coord: 105..444
e-value: 3.5E-147
score: 491.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..95
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 46..69
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 444..477
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..95
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 507..529
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 492..529
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 459..473
NoneNo IPR availablePANTHERPTHR33101:SF10ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 12coord: 11..547
IPR005512PRONE domainPFAMPF03759PRONEcoord: 100..447
e-value: 4.0E-173
score: 575.3
IPR005512PRONE domainPROSITEPS51334PRONEcoord: 91..453
score: 115.632812
IPR038937Rop guanine nucleotide exchange factorPANTHERPTHR33101ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1coord: 11..547

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G007050.1CmaCh08G007050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity