CmaCh08G005770 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh08G005770
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionMitogen-activated protein kinase kinase kinase
LocationCma_Chr08: 3293551 .. 3294732 (-)
RNA-Seq ExpressionCmaCh08G005770
SyntenyCmaCh08G005770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAGAAAATCAACCCCATCACAACACAACGCCACTCCCGCCACCTCTTCCAACGACGGAATCCGGTGGAAAAGAGGCCCGGTGATCGGAAAAGGAACCTTTGGATCCGTTTTCATGGCTTCTCTTAAACCACCCATCTCCAAATACAGCATTTTCCCTCCGGTAATTGCCGTAAAATCCGCTGAAATTTCCGACTCCCAAACCCTACAAAAGGAGAAACAAATTTACGATAATTTAAAACCCTGCAATTCCTTGATCAAATGCTTTGGTGATGAGATCACCACCGACGTCAATGGCCGCATGATTTATAATTTGCTTCTCGAATTCGCCCCCGCTGGAACCCTAGCCGACCAAATCAAGAACACAGGTAGCAATTATTGGATTATGAAAGTTCCATATCAGCTAATTTAGAGAATTGACAGAGTTTATAATCAAGGAATACTTTTCCATTGGTATGAGGCCATGAGAACTTATGCTCAAAGTAGACAATATGTTCATGGAACAGCAATTACATAAAATAAAAAAAGAATTCATTTAATTATGAATATAAAATTTTAAAATCTTATATTTATTTGATTAGGTGGAAAAGGTTTACCGGAAGAAGAGGTTCGGAGGTACACAAGGTCAATAATCAAAGGGTTAATTCATATTCATAAGAATCAATACATTCATTGTGATTTGAAGCCGGCGAATATCTTATTACTGCCGAGGAATTCACGAGTTGAGGCAAAGATCGGCGATTTAGGGTTGGCGAGGAGAATGAGCAAGACAAAGCGAGGGTATTGTTTGGGAGGAACATTGATGTGTATGGCGCCGGAGACGTTGATCGACAACGTGCAAGAATCGCCGAGTGATATTTGGGCACTCGGCTGCGTCGTGCTCGAAATGCTCACTGGTAACTGTCCGTGGCCTGCCACGGACAGGGACGGGATTGGGAAATATATTGTAGAGAAGCACGGGATGCCGATGATTCCCGAAGGGTTGTCGGAGGAGGCGAGAGGTTTTTTGAAGGGTTGTTTTGTGAGGAAGCCGGAGTTTAGATTCACGGCTGAGATGCTGATGTTTGTGCCGTTTGTGGCGGCGGTGGCGGCTGAGGAGGAAGAAGATTTAGTGACGGCTCCAACATTTGTGGAGAAGAGGAGGAGTTCTAAGAACTCCAATTCAAGCTGTGTGAATTAA

mRNA sequence

ATGAAGAGAAAATCAACCCCATCACAACACAACGCCACTCCCGCCACCTCTTCCAACGACGGAATCCGGTGGAAAAGAGGCCCGGTGATCGGAAAAGGAACCTTTGGATCCGTTTTCATGGCTTCTCTTAAACCACCCATCTCCAAATACAGCATTTTCCCTCCGGTAATTGCCGTAAAATCCGCTGAAATTTCCGACTCCCAAACCCTACAAAAGGAGAAACAAATTTACGATAATTTAAAACCCTGCAATTCCTTGATCAAATGCTTTGGTGATGAGATCACCACCGACGTCAATGGCCGCATGATTTATAATTTGCTTCTCGAATTCGCCCCCGCTGGAACCCTAGCCGACCAAATCAAGAACACAGGTGGAAAAGGTTTACCGGAAGAAGAGGTTCGGAGGTACACAAGGTCAATAATCAAAGGGTTAATTCATATTCATAAGAATCAATACATTCATTGTGATTTGAAGCCGGCGAATATCTTATTACTGCCGAGGAATTCACGAGTTGAGGCAAAGATCGGCGATTTAGGGTTGGCGAGGAGAATGAGCAAGACAAAGCGAGGGTATTGTTTGGGAGGAACATTGATGTGTATGGCGCCGGAGACGTTGATCGACAACGTGCAAGAATCGCCGAGTGATATTTGGGCACTCGGCTGCGTCGTGCTCGAAATGCTCACTGGTAACTGTCCGTGGCCTGCCACGGACAGGGACGGGATTGGGAAATATATTGTAGAGAAGCACGGGATGCCGATGATTCCCGAAGGGTTGTCGGAGGAGGCGAGAGGTTTTTTGAAGGGTTGTTTTGTGAGGAAGCCGGAGTTTAGATTCACGGCTGAGATGCTGATGTTTGTGCCGTTTGTGGCGGCGGTGGCGGCTGAGGAGGAAGAAGATTTAGTGACGGCTCCAACATTTGTGGAGAAGAGGAGGAGTTCTAAGAACTCCAATTCAAGCTGTGTGAATTAA

Coding sequence (CDS)

ATGAAGAGAAAATCAACCCCATCACAACACAACGCCACTCCCGCCACCTCTTCCAACGACGGAATCCGGTGGAAAAGAGGCCCGGTGATCGGAAAAGGAACCTTTGGATCCGTTTTCATGGCTTCTCTTAAACCACCCATCTCCAAATACAGCATTTTCCCTCCGGTAATTGCCGTAAAATCCGCTGAAATTTCCGACTCCCAAACCCTACAAAAGGAGAAACAAATTTACGATAATTTAAAACCCTGCAATTCCTTGATCAAATGCTTTGGTGATGAGATCACCACCGACGTCAATGGCCGCATGATTTATAATTTGCTTCTCGAATTCGCCCCCGCTGGAACCCTAGCCGACCAAATCAAGAACACAGGTGGAAAAGGTTTACCGGAAGAAGAGGTTCGGAGGTACACAAGGTCAATAATCAAAGGGTTAATTCATATTCATAAGAATCAATACATTCATTGTGATTTGAAGCCGGCGAATATCTTATTACTGCCGAGGAATTCACGAGTTGAGGCAAAGATCGGCGATTTAGGGTTGGCGAGGAGAATGAGCAAGACAAAGCGAGGGTATTGTTTGGGAGGAACATTGATGTGTATGGCGCCGGAGACGTTGATCGACAACGTGCAAGAATCGCCGAGTGATATTTGGGCACTCGGCTGCGTCGTGCTCGAAATGCTCACTGGTAACTGTCCGTGGCCTGCCACGGACAGGGACGGGATTGGGAAATATATTGTAGAGAAGCACGGGATGCCGATGATTCCCGAAGGGTTGTCGGAGGAGGCGAGAGGTTTTTTGAAGGGTTGTTTTGTGAGGAAGCCGGAGTTTAGATTCACGGCTGAGATGCTGATGTTTGTGCCGTTTGTGGCGGCGGTGGCGGCTGAGGAGGAAGAAGATTTAGTGACGGCTCCAACATTTGTGGAGAAGAGGAGGAGTTCTAAGAACTCCAATTCAAGCTGTGTGAATTAA

Protein sequence

MKRKSTPSQHNATPATSSNDGIRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQTLQKEKQIYDNLKPCNSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYTRSIIKGLIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARRMSKTKRGYCLGGTLMCMAPETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATDRDGIGKYIVEKHGMPMIPEGLSEEARGFLKGCFVRKPEFRFTAEMLMFVPFVAAVAAEEEEDLVTAPTFVEKRRSSKNSNSSCVN
Homology
BLAST of CmaCh08G005770 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.5e-53
Identity = 114/275 (41.45%), Postives = 160/275 (58.18%), Query Frame = 0

Query: 22  IRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQTLQKEKQIYDNLK 81
           + W RG  IG GTF +V  A+          FP +IAVKS +   + +L  EK + D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKS---RNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 82  PCNSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYTRSII 141
            C  +I+C+G++ T + NG  ++NLLLE+A  G+LA  +K  GG+GLPE  VRR+T S++
Sbjct: 61  DCPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 142 KGLIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARR----MSKTKRGYCLGGTL 201
           +GL HIH   + HCD+K ANILL    S    KI D GLA R    ++  ++   + GT 
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS---VKIADFGLAMRVDGDLTALRKSVEIRGTP 180

Query: 202 MCMAPETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATDRDGIGKYIVE---KHGMPMI 261
           + MAPE + DN   S +D+WALGC V+EM +G   W   +       ++       +P I
Sbjct: 181 LYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKI 240

Query: 262 PEGLSEEARGFLKGCFVRKPEFRFTAEMLMFVPFV 290
           PE LSEE + FL  CFV+ P  R+TAEML+   FV
Sbjct: 241 PEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFV 268

BLAST of CmaCh08G005770 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 4.1e-40
Identity = 98/272 (36.03%), Postives = 152/272 (55.88%), Query Frame = 0

Query: 22  IRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQTLQKEKQIYDNLK 81
           + W RG ++G+G+  +V+ A+             ++AVKS+E+  S+ LQ+E +I  +L 
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNS-------DEILAVKSSEVHRSEFLQREAKILSSLS 60

Query: 82  PCNSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYTRSII 141
               +I   G E   + NG ++YNLL+E+AP GTL D     GG+ + E  V +YTR I+
Sbjct: 61  -SPYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGR-VDETRVVKYTRDIL 120

Query: 142 KGLIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARRMSKTKRGYCLGGTLMCMA 201
           KGL +IH    +HCD+K +N+++   + + EAKI D G A+R+        + GT   MA
Sbjct: 121 KGLEYIHSKGIVHCDVKGSNVVI---SEKGEAKIADFGCAKRVDPVFESPVM-GTPAFMA 180

Query: 202 PETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATDRDGIGKYIVEKHG----MPMIPEG 261
           PE      Q   SDIWA+GC ++EM+TG+ PW   D       ++ + G     P +P  
Sbjct: 181 PEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCL 240

Query: 262 LSEEARGFLKGCFVRKPEFRFTAEMLMFVPFV 290
           L+EEA+ FL+ C  R+   R+TA  L+  PF+
Sbjct: 241 LAEEAKDFLEKCLKREANERWTATQLLNHPFL 259

BLAST of CmaCh08G005770 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 6.3e-33
Identity = 109/295 (36.95%), Postives = 156/295 (52.88%), Query Frame = 0

Query: 22  IRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQTLQKEKQIYDNLK 81
           + W RG  +G+G+  +V  A+              +AVKSAE   S+ LQ+E +I  +L 
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHES-------GETLAVKSAEFHRSEFLQREAKILSSLN 60

Query: 82  PCNSLIKCFGDEITTDV---NGR-MIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYT 141
               +I   G EIT +    NG    Y+LL+E+AP GTL D     GG  + E  V +YT
Sbjct: 61  -SPYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGG-FIDEARVVKYT 120

Query: 142 RSIIKGLIHIHKNQYI-HCDLKPANILLLPRNSRVEAKIGDLGLARRMSKTKRGYCLGGT 201
           R I+ GL +IH ++ I HCD+K +N+L+       EAKI D G A+ + + +    + GT
Sbjct: 121 RQILLGLEYIHNSKGIAHCDIKGSNVLV---GENGEAKIADFGCAKWV-EPEITEPVRGT 180

Query: 202 LMCMAPETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATD-RDGIG-KYIVEKHG-MPM 261
              MAPE      Q   SDIWA+GC V+EM+TG+ PW   D  D +   Y V   G +P 
Sbjct: 181 PAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPE 240

Query: 262 IPEGLSEEARGFLKGCFVRKPEFRFTAEMLMFVPFVAAVAAEEEEDLVT-APTFV 308
           +P  L+E+A+ FL  C  ++   R+TA  L+  PF+     E    LVT +PT V
Sbjct: 241 LPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSV 282

BLAST of CmaCh08G005770 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 3.5e-31
Identity = 86/276 (31.16%), Postives = 143/276 (51.81%), Query Frame = 0

Query: 22  IRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQT----LQKEKQIY 81
           I W++G +IG+G FG+V+M          ++   +IA   A    +Q     L++E ++ 
Sbjct: 67  ISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLL 126

Query: 82  DNLKPCNSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYT 141
            NL   N +++  G     D       N+LLEF P G+++  ++  G    PE  VR YT
Sbjct: 127 KNLSHPN-IVRYLGTVREDDT-----LNILLEFVPGGSISSLLEKFG--PFPESVVRTYT 186

Query: 142 RSIIKGLIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARRMSK--TKRG-YCLG 201
           R ++ GL ++H +  +H D+K ANIL+   +++   K+ D G ++++++  T  G   + 
Sbjct: 187 RQLLLGLEYLHNHAIMHRDIKGANILV---DNKGCIKLADFGASKQVAELATMTGAKSMK 246

Query: 202 GTLMCMAPETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATDRDGIGKYIV-EKHGMPM 261
           GT   MAPE ++       +DIW++GC V+EM+TG  PW    ++    + +      P 
Sbjct: 247 GTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPP 306

Query: 262 IPEGLSEEARGFLKGCFVRKPEFRFTAEMLMFVPFV 290
           IP+ LS +A+ FL  C    P  R TA  L+  PFV
Sbjct: 307 IPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFV 331

BLAST of CmaCh08G005770 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.5e-31
Identity = 91/289 (31.49%), Postives = 146/289 (50.52%), Query Frame = 0

Query: 14  PATSSNDG---IRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQT- 73
           PA +  D    IRW++G +IG G FG V+M          +I    IA+  A    +Q  
Sbjct: 67  PAKARKDDTPPIRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAH 126

Query: 74  ---LQKEKQIYDNLKPCNSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGK 133
              L++E  +  NL   N +++  G   T    G +  N+LLEF P G+++  +   G  
Sbjct: 127 VRELEEEVNLLKNLSHPN-IVRYLG---TAREAGSL--NILLEFVPGGSISSLLGKFG-- 186

Query: 134 GLPEEEVRRYTRSIIKGLIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARR--- 193
             PE  +R YT+ ++ GL ++HKN  +H D+K ANIL+   +++   K+ D G +++   
Sbjct: 187 SFPESVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILV---DNKGCIKLADFGASKKVVE 246

Query: 194 MSKTKRGYCLGGTLMCMAPETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATDRDGIGK 253
           ++       + GT   MAPE ++       +DIW++GC ++EM TG  PW    ++    
Sbjct: 247 LATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAAL 306

Query: 254 Y-IVEKHGMPMIPEGLSEEARGFLKGCFVRKPEFRFTAEMLMFVPFVAA 292
           + I      P IPE LS E++ FL  C  ++P  R +A  L+  PFV A
Sbjct: 307 FHIGTTKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTA 344

BLAST of CmaCh08G005770 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 218.8 bits (556), Expect = 6.5e-57
Identity = 126/302 (41.72%), Postives = 176/302 (58.28%), Query Frame = 0

Query: 22  IRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQTLQKEKQIYDNL- 81
           + W RG  IG GTF +V +A+     S    FPP++AVKSA+   + +L  EK + DNL 
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDS--GEFPPLMAVKSADSYGAASLANEKSVLDNLG 60

Query: 82  KPCNSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYTRSI 141
             CN +++CFG++ T + NG  ++NL LE+A  G+L   +K   G+G+PE  VRR+T S+
Sbjct: 61  DDCNEIVRCFGEDRTVE-NGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSV 120

Query: 142 IKGLIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARR---MSKTKRGYCLGGTL 201
           ++GL HIH N + HCDLK  NILL    +    KI D GLA+R   ++    G  + GT 
Sbjct: 121 LRGLRHIHANGFAHCDLKLGNILLFGDGA---VKIADFGLAKRIGDLTALNYGVQIRGTP 180

Query: 202 MCMAPETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATD---------RDGIGKYIVEK 261
           + MAPE++ DN   S  D+WALGCVV+EM +G   W   +         R G+G  +   
Sbjct: 181 LYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEV--- 240

Query: 262 HGMPMIPEGLSEEARGFLKGCFVRKPEFRFTAEMLMFVPFVAA-----VAAEEEEDLVTA 306
              PMIPE LSE+ R FL  CFV+ P+ R+TAEML+  PFV       V  +EE+ +V  
Sbjct: 241 ---PMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNM 290

BLAST of CmaCh08G005770 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 209.5 bits (532), Expect = 3.9e-54
Identity = 114/275 (41.45%), Postives = 160/275 (58.18%), Query Frame = 0

Query: 22  IRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQTLQKEKQIYDNLK 81
           + W RG  IG GTF +V  A+          FP +IAVKS +   + +L  EK + D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKS---RNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 82  PCNSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYTRSII 141
            C  +I+C+G++ T + NG  ++NLLLE+A  G+LA  +K  GG+GLPE  VRR+T S++
Sbjct: 61  DCPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 142 KGLIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARR----MSKTKRGYCLGGTL 201
           +GL HIH   + HCD+K ANILL    S    KI D GLA R    ++  ++   + GT 
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS---VKIADFGLAMRVDGDLTALRKSVEIRGTP 180

Query: 202 MCMAPETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATDRDGIGKYIVE---KHGMPMI 261
           + MAPE + DN   S +D+WALGC V+EM +G   W   +       ++       +P I
Sbjct: 181 LYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKI 240

Query: 262 PEGLSEEARGFLKGCFVRKPEFRFTAEMLMFVPFV 290
           PE LSEE + FL  CFV+ P  R+TAEML+   FV
Sbjct: 241 PEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFV 268

BLAST of CmaCh08G005770 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 208.4 bits (529), Expect = 8.7e-54
Identity = 121/285 (42.46%), Postives = 171/285 (60.00%), Query Frame = 0

Query: 22  IRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQTLQKEKQIYDNLK 81
           + W R   IG G+F +V +A+     SK   FP ++AVKS+ +  S  L+ E+ + D+L 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSK--AFPSLMAVKSSGVVCSAALRNERDVLDDLG 60

Query: 82  PCNSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYTRSII 141
            C+ +++CFG+  T + NG  IYNL LE+A  G+LAD+IK++ G+ LPE EVRR+TRSI+
Sbjct: 61  DCSEIVRCFGEGRTVE-NGEEIYNLFLEYASGGSLADRIKSS-GEALPEFEVRRFTRSIV 120

Query: 142 KGLIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARRMSKTKRGYC--LGGTLMC 201
           KGL HIH N + HCD+K  N+L+       + KI D GLA+R S      C  + GT + 
Sbjct: 121 KGLCHIHGNGFTHCDIKLENVLVFGDG---DVKISDFGLAKRRSGE---VCVEIRGTPLY 180

Query: 202 MAPETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATD--RDGIGKYIV---EKHGMPMI 261
           MAPE++     ESP+DIWALGC V+EM +G   W   D   + +   +V       +P I
Sbjct: 181 MAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRI 240

Query: 262 PEGLSEEARGFLKGCFVRKPEFRFTAEMLMFVPFVAAVAAEEEED 300
           P  LSEE + F+  CFV+    R+TAEML+  PF+A      EED
Sbjct: 241 PVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEED 275

BLAST of CmaCh08G005770 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 190.3 bits (482), Expect = 2.5e-48
Identity = 109/292 (37.33%), Postives = 164/292 (56.16%), Query Frame = 0

Query: 22  IRWKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQTLQKEKQIYDNLK 81
           I W RGP+IG+G+  +V +A         S    + AVKSA++S S  LQKE+ I   L 
Sbjct: 3   INWTRGPIIGRGSTATVSIA--------ISSSGELFAVKSADLSSSSLLQKEQSILSTLS 62

Query: 82  PCNSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYTRSII 141
             + ++K  G  +T + NG ++YN+L+E+   G L D IKN+GGK LPE E+R YTR I+
Sbjct: 63  SPH-MVKYIGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGGK-LPEPEIRSYTRQIL 122

Query: 142 KGLIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARRMSKTKRGYCLGGTLMCMA 201
            GL+++H+   +HCDLK  N+L+         KI D+G A+ + K++      GT   MA
Sbjct: 123 NGLVYLHERGIVHCDLKSHNVLV---EENGVLKIADMGCAKSVDKSE----FSGTPAFMA 182

Query: 202 PETLIDNVQESPSDIWALGCVVLEMLTGNCPWPATDRDGIGKYIVEKHG-MPMIPEGLSE 261
           PE      Q  P+D+WALGC ++EM+TG+ PWP  +      Y +   G  P IP  +S+
Sbjct: 183 PEVARGEEQRFPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISD 242

Query: 262 EARGFLKGCFVRKPEFRFTAEMLMFVPFVAAVAAEEEEDLVTAPTFVEKRRS 313
           +A+ FLK C     + R+T E L+  PF+     +++E+  T+     K  S
Sbjct: 243 KAKDFLKNCLKEDQKQRWTVEELLKHPFL-----DDDEESQTSDCLKNKTSS 271

BLAST of CmaCh08G005770 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 178.7 bits (452), Expect = 7.4e-45
Identity = 107/277 (38.63%), Postives = 157/277 (56.68%), Query Frame = 0

Query: 24  WKRGPVIGKGTFGSVFMASLKPPISKYSIFPPVIAVKSAEISDSQTLQKEKQIYDNLKPC 83
           W RGP+IG+G+  +V +      I+    F    AVKSAE S S  LQ+E+ I   L   
Sbjct: 6   WIRGPIIGRGSTATVSLG-----ITNSGDF---FAVKSAEFSSSAFLQREQSILSKLS-S 65

Query: 84  NSLIKCFGDEITTDVNGRMIYNLLLEFAPAGTLADQIKNTGGKGLPEEEVRRYTRSIIKG 143
             ++K  G  +T + N +++YNLL+E+   G+L D IKN+GGK LPE  +R YTR I+KG
Sbjct: 66  PYIVKYIGSNVTKE-NDKLMYNLLMEYVSGGSLHDLIKNSGGK-LPEPLIRSYTRQILKG 125

Query: 144 LIHIHKNQYIHCDLKPANILLLPRNSRVEAKIGDLGLARRMSKTKRGYCLGGTLMCMAPE 203
           L+++H    +HCD+K  N+++        AKI DLG A+ + + +      GT   M+PE
Sbjct: 126 LMYLHDQGIVHCDVKSQNVMI----GGEIAKIVDLGCAKTVEENE-NLEFSGTPAFMSPE 185

Query: 204 TLIDNVQESPSDIWALGCVVLEMLTGNCPWPATDRDGIGKYIVEKHG-MPMIPEGLSEEA 263
                 Q  P+D+WALGC V+EM TG+ PWP  +      Y +   G  P+IP  LSE+ 
Sbjct: 186 VARGEEQSFPADVWALGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKG 245

Query: 264 RGFLKGCFVRKPEFRFTAEMLMFVPFVAAVAAEEEED 300
           + FL+ C  + P+ R+T E L+  PF+      +EED
Sbjct: 246 QDFLRKCLRKDPKQRWTVEELLQHPFL------DEED 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SND65.5e-5341.45Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808884.1e-4036.03Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP56.3e-3336.95Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
O220403.5e-3131.16Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Q405414.5e-3131.49Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
Match NameE-valueIdentityDescription
AT5G67080.16.5e-5741.72mitogen-activated protein kinase kinase kinase 19 [more]
AT3G50310.13.9e-5441.45mitogen-activated protein kinase kinase kinase 20 [more]
AT4G36950.18.7e-5442.46mitogen-activated protein kinase kinase kinase 21 [more]
AT4G26890.12.5e-4837.33mitogen-activated protein kinase kinase kinase 16 [more]
AT5G55090.17.4e-4538.63mitogen-activated protein kinase kinase kinase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 24..289
e-value: 8.0E-48
score: 174.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 27..288
e-value: 7.4E-43
score: 146.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 24..289
score: 36.385876
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 13..112
e-value: 9.7E-7
score: 30.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 115..321
e-value: 9.3E-45
score: 155.0
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 101..235
e-value: 2.7E-9
score: 33.7
NoneNo IPR availablePIRSRPIRSR631048-52PIRSR631048-52coord: 142..230
e-value: 5.3E-8
score: 29.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR48011:SF56MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKEcoord: 21..306
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 21..306
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 30..60
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 152..164
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 23..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G005770.1CmaCh08G005770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity