CmaCh08G004780 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh08G004780 vs. ExPASy Swiss-Prot
Match: Q93XK2 (Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1)

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 577/860 (67.09%), Postives = 695/860 (80.81%), Query Frame = 0

Query: 1   MAPPNDPSTLTSSPLKSEGLDNLVDFSDGKLSVKGVSLLSDVPSNVFFSSFSSICQPSD- 60
           MAPP   ++ TS+ +K+E   ++ D S+ K  VKG  L  DVP NV F SFSSIC+PS+ 
Sbjct: 1   MAPP--LNSTTSNLIKTE---SIFDLSERKFKVKGFPLFHDVPENVSFRSFSSICKPSES 60

Query: 61  -APLPLLQRVHSLSHKGGFLGFDQTKSSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGN 120
            AP  LLQ+V + SHKGGF GF     SDRL NS+G F G++F+S+FRFKTWWST W+G 
Sbjct: 61  NAPPSLLQKVLAYSHKGGFFGFSHETPSDRLMNSIGSFNGKDFLSIFRFKTWWSTQWIGK 120

Query: 121 SGSDLQMETQWFMLNVPEIKSYVVFIPIIEGSFRSAIHPGVDGHVMICAESGSTHVKTSS 180
           SGSDLQMETQW ++ VPE KSYVV IPIIE  FRSA+ PG + HV I AESGST VK S+
Sbjct: 121 SGSDLQMETQWILIEVPETKSYVVIIPIIEKCFRSALFPGFNDHVKIIAESGSTKVKEST 180

Query: 181 FDAITYVHVSDNPYKLMKEAYAAIRVHMNTFRLLEEKPVTHLLDKFGWCTWDAFYLTVDP 240
           F++I YVH S+NPY LMKEAY+AIRVH+N+FRLLEEK + +L+DKFGWCTWDAFYLTV+P
Sbjct: 181 FNSIAYVHFSENPYDLMKEAYSAIRVHLNSFRLLEEKTIPNLVDKFGWCTWDAFYLTVNP 240

Query: 241 VGIWNGVNDFAEGGVSPRFLIIDDGWQSINIDGEDPTRDAKNLVLGGTQMTARLYRFDEC 300
           +GI++G++DF++GGV PRF+IIDDGWQSI+ DG DP  DAKNLVLGG QM+ RL+RFDEC
Sbjct: 241 IGIFHGLDDFSKGGVEPRFVIIDDGWQSISFDGYDPNEDAKNLVLGGEQMSGRLHRFDEC 300

Query: 301 EKFRKYKGGSLSGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDITKFETKIH 360
            KFRKY+ G L GPN+P +DP     LI K IE E   K R++AI S  +D+ + E+KI 
Sbjct: 301 YKFRKYESGLLLGPNSPPYDPNNFTDLILKGIEHEKLRKKREEAISSKSSDLAEIESKIK 360

Query: 361 KFKEELNAIFGKEQDSGCSSCSCKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAGAWGGV 420
           K  +E++ +FG EQ S       K  + G+KAFT+DLRTKFKGLDDV+VWHAL GAWGGV
Sbjct: 361 KVVKEIDDLFGGEQFSSGEKSEMK-SEYGLKAFTKDLRTKFKGLDDVYVWHALCGAWGGV 420

Query: 421 RPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVG 480
           RP  THL++KIVPCKLSPGLDGTM DLAVV+I + S+GLVHP QA++ +DSMHSYL++ G
Sbjct: 421 RPETTHLDTKIVPCKLSPGLDGTMEDLAVVEISKASLGLVHPSQANELYDSMHSYLAESG 480

Query: 481 ITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTKSLHKNFKGTGLFSSMQQCNDFFFLG 540
           ITGVKVDV+H+LEYV +EYGGRVDLAK YY+GLTKS+ KNF G G+ +SMQ CNDFFFLG
Sbjct: 481 ITGVKVDVIHSLEYVCDEYGGRVDLAKVYYEGLTKSIVKNFNGNGMIASMQHCNDFFFLG 540

Query: 541 TKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLC 600
           TKQ S+GRVGDDFWFQDPNGDPMG +WLQGVHMIHC+YNS+WMGQ+IQPDWDMFQSDH+C
Sbjct: 541 TKQISMGRVGDDFWFQDPNGDPMGSFWLQGVHMIHCSYNSLWMGQMIQPDWDMFQSDHVC 600

Query: 601 AKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFD 660
           AKFHAGSRAICGGP+YVSD+VG H+FDLIK+LV+PDGTIP+C +F LPTRDCLFKNPLFD
Sbjct: 601 AKFHAGSRAICGGPIYVSDNVGSHDFDLIKKLVFPDGTIPKCIYFPLPTRDCLFKNPLFD 660

Query: 661 SKTVLKIWNFNKYGGVIGAFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVSDVEWDQKPE 720
             TVLKIWNFNKYGGVIGAFNCQGAGWDP  Q+ +G PECYKP+  TVHV++VEWDQK E
Sbjct: 661 HTTVLKIWNFNKYGGVIGAFNCQGAGWDPIMQKFRGFPECYKPIPGTVHVTEVEWDQKEE 720

Query: 721 AAPMGNFTEYIVYLNQAEQILHTTPKFEPLKVTIQPSTFEIFSFIPLRKLNSNIKFAPIG 780
            + +G   EY+VYLNQAE++   T K EP++ TIQPSTFE++SF+P+ KL   IKFAPIG
Sbjct: 721 TSHLGKAEEYVVYLNQAEELSLMTLKSEPIQFTIQPSTFELYSFVPVTKLCGGIKFAPIG 780

Query: 781 LKEMFNSSGTIQHLKYNENGVEMKVKGGGNFIAYSSASPKKCISNGVEVEFEWDDSDGKL 840
           L  MFNS GT+  L+Y  NG ++KVKGGG+F+AYSS SPKK   NG EV+FEW   DGKL
Sbjct: 781 LTNMFNSGGTVIDLEYVGNGAKIKVKGGGSFLAYSSESPKKFQLNGCEVDFEW-LGDGKL 840

Query: 841 SFDLPWIEEAKGVSNVEILF 859
             ++PWIEEA GVS++EI F
Sbjct: 841 CVNVPWIEEACGVSDMEIFF 853

BLAST of CmaCh08G004780 vs. ExPASy Swiss-Prot
Match: Q9SYJ4 (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=RFS4 PE=2 SV=3)

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 514/847 (60.68%), Postives = 645/847 (76.15%), Query Frame = 0

Query: 22  NLVDFSDGKLSVK-GVSLLSDVPSNVFFSSFSSICQPSDAPLPLLQRVHSLSHKGGFLGF 81
           N  + S+G L  K    +L DVP NV F+ FSS    +DAPLP+L RV + +HKGGFLGF
Sbjct: 32  NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 82  DQTKSSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWFMLNVPEIKSY 141
            +   SDRLTNSLG+F+ REF+S+FRFK WWST W+G SGSDLQ ETQW ML +PEI SY
Sbjct: 92  TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEIDSY 151

Query: 142 VVFIPIIEGSFRSAIHPGVDGHVMICAESGSTHVKTSSFDAITYVHVSDNPYKLMKEAYA 201
           V  IP IEG+FR+++ PG  G+V+ICAESGST VK SSF +I Y+H+ DNPY LMKEA++
Sbjct: 152 VAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFS 211

Query: 202 AIRVHMNTFRLLEEKPVTHLLDKFGWCTWDAFYLTVDPVGIWNGVNDFAEGGVSPRFLII 261
           A+RVHMNTF+LLEEK +  ++DKFGWCTWDA YLTVDP  IW GV +F +GGV P+F+II
Sbjct: 212 ALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVII 271

Query: 262 DDGWQSINIDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLSGPNAPSFDPK 321
           DDGWQSIN DG++  +DA+NLVLGG QMTARL  F EC+KFR YKGGS    +A  F+P 
Sbjct: 272 DDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPL 331

Query: 322 KPKLLIAKAIEIEHAEKDRDKAI-GSGVTDITKFETKIHKFKEELNAIFGK-EQDSGCSS 381
           KPK+LI KA E   A   R K +  SG  D+T+ + KI    EELNA+F + E++    S
Sbjct: 332 KPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLGS 391

Query: 382 CSCKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAGAWGGVRPGA-THLNSKIVPCKLSPG 441
               G  SGM AFT+DLR +FK LDD++VWHAL GAW GVRP     L +K+ P +LSP 
Sbjct: 392 DDVSG--SGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAPFELSPS 451

Query: 442 LDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHTLEYVSEEY 501
           L  TM DLAV K++E  IGLVHP +A +F+DSMHSYL+ VG+TG K+DV  TLE ++EE+
Sbjct: 452 LGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQTLESLAEEH 511

Query: 502 GGRVDLAKAYYKGLTKSLHKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVGDDFWFQDPN 561
           GGRV+LAKAYY GLT+S+ KNF GT + +SMQQCN+FFFL TKQ SIGRVGDDFW+QDP 
Sbjct: 512 GGRVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGDDFWWQDPY 571

Query: 562 GDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSD 621
           GDP GVYWLQGVHMIHC+YNS+WMGQ+IQPDWDMFQSDH+CA++HA SRAICGGPVY+SD
Sbjct: 572 GDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAICGGPVYLSD 631

Query: 622 SVG--GHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDSKTVLKIWNFNKYGGVI 681
            +G   HNFDLIK+L + DGTIPRC H+ALPTRD LFKNPLFD +++LKI+NFNK+GGVI
Sbjct: 632 HLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFNFNKFGGVI 691

Query: 682 GAFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVSDVEWDQKPEAA-PMGNFT-EYIVYLN 741
           G FNCQGAGW P+E R KG+ ECY  +S TVHVSD+EWDQ PEAA     +T +Y+VY  
Sbjct: 692 GTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAAGSQVTYTGDYLVYKQ 751

Query: 742 QAEQILHTTPKFEPLKVTIQPSTFEIFSFIPLRKL-NSNIKFAPIGLKEMFNSSGTIQHL 801
           Q+E+IL    K E +K+T++PS F++ SF+P+ +L +S ++FAP+GL  MFN  GT+Q +
Sbjct: 752 QSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFAPLGLINMFNCVGTVQDM 811

Query: 802 KY-NENGVEMKVKGGGNFIAYSSASPKKCISNGVEVEFEWDDSDGKLSFDLPWIEEAKGV 859
           K   +N + + VKG G F+AYSS++P KC  N  E EF+W++  GKLSF +PW+EE+ G+
Sbjct: 812 KVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEETGKLSFFVPWVEESGGI 871

BLAST of CmaCh08G004780 vs. ExPASy Swiss-Prot
Match: Q9FND9 (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=RFS5 PE=1 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 8.2e-213
Identity = 382/861 (44.37%), Postives = 516/861 (59.93%), Query Frame = 0

Query: 16  KSEGLDNLVDFS------DGKLSVKGVSLLSDVPSNVFFSSFSSICQPSDAPLPLLQRVH 75
           KS+   N VDF+      D  L   G  +L+DVP NV  +S   +      PL +     
Sbjct: 8   KSDSGINGVDFTEKFRLEDSTLLANGQVVLTDVPVNVTLTSSPYLVDKDGVPLDV----- 67

Query: 76  SLSHKGGFLGFD-QTKSSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQ 135
                G F+GF+   +       S+GK K   F+S+FRFK WW+T WVG++G D++ ETQ
Sbjct: 68  ---SAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQ 127

Query: 136 WFMLNVPEIKS---------YVVFIPIIEGSFRSAIHPGVDGHVMICAESGSTHVKTSSF 195
             +L+     S         YV+ +P++EGSFRS+   G D  V +C ESGST V  S F
Sbjct: 128 IIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEF 187

Query: 196 DAITYVHVSDNPYKLMKEAYAAIRVHMNTFRLLEEKPVTHLLDKFGWCTWDAFYLTVDPV 255
             I YVH  D+P+KL+K+A   IRVHMNTF+LLEEK    ++DKFGWCTWDAFYLTV+P 
Sbjct: 188 RQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPD 247

Query: 256 GIWNGVNDFAEGGVSPRFLIIDDGWQSINIDGEDPTRDAKNLVLGGTQMTARLYRFDECE 315
           G+  GV    +GG  P  ++IDDGWQSI  D +    +  N+ + G QM  RL +F+E  
Sbjct: 248 GVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENH 307

Query: 316 KFRKYKGGSLSGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDITKFETKIHK 375
           KF+ Y                                                       
Sbjct: 308 KFKDY------------------------------------------------------- 367

Query: 376 FKEELNAIFGKEQDSGCSSCSCKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAGAWGGVR 435
                  +  K+Q           +D GMKAF RDL+ +F  +D ++VWHAL G WGG+R
Sbjct: 368 -------VSPKDQ-----------NDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLR 427

Query: 436 PGATHL-NSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVG 495
           P A  L  S I+  +LSPGL  TM DLAV KIIE  IG   PD A +F++ +HS+L   G
Sbjct: 428 PEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAG 487

Query: 496 ITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTKSLHKNFKGTGLFSSMQQCNDFFFLG 555
           I GVKVDV+H LE + ++YGGRVDLAKAY+K LT S++K+F G G+ +SM+ CNDF FLG
Sbjct: 488 IDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLG 547

Query: 556 TKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLC 615
           T+  S+GRVGDDFW  DP+GDP G +WLQG HM+HCAYNS+WMG  IQPDWDMFQS H C
Sbjct: 548 TEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPC 607

Query: 616 AKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFD 675
           A+FHA SRAI GGP+Y+SD VG H+FDL+K+LV P+G+I RC+++ALPTRD LF++PL D
Sbjct: 608 AEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHD 667

Query: 676 SKTVLKIWNFNKYGGVIGAFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVSDVEWDQKPE 735
            KT+LKIWN NKY GVIGAFNCQG GW  + +R +   EC   ++ T    DVEW+    
Sbjct: 668 GKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSS 727

Query: 736 AAPMGNFTEYIVYLNQAEQILHTTPKFEPLKVTIQPSTFEIFSFIPLRKLNSN-IKFAPI 795
              + N  E+ ++L+Q++++L  +   + L++T++P  FE+ +  P+  +  N ++FAPI
Sbjct: 728 PISIANVEEFALFLSQSKKLL-LSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPI 783

Query: 796 GLKEMFNSSGTIQHLKYNENGVEMKVKGGGNFIAYSSASPKKCISNGVEVEFEWDDSDGK 855
           GL  M N+SG I+ L YN+  VE+ V G G F  Y+S  P  C+ +G  VEF ++DS   
Sbjct: 788 GLVNMLNTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYEDS--M 783

Query: 856 LSFDLPWIEEAKGVSNVEILF 859
           +   +PW     G+S+++ LF
Sbjct: 848 VMVQVPW-SGPDGLSSIQYLF 783

BLAST of CmaCh08G004780 vs. ExPASy Swiss-Prot
Match: Q8VWN6 (Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 4.2e-209
Identity = 384/887 (43.29%), Postives = 519/887 (58.51%), Query Frame = 0

Query: 1   MAPPNDPSTLT-----------SSPLKSEGLDNLVDFSDGKLSVKGVSLLSDVPSNVFFS 60
           MAPP+   T T           +SPL S  LD   +F      V G   L+ VP N+  +
Sbjct: 1   MAPPSITKTATQQDVISTVDIGNSPLLSISLDQSRNF-----LVNGHPFLTQVPPNITTT 60

Query: 61  SFSSICQPSDAPLPLL----------QRVHSLSHKGGFLGFDQTKSSDRLTNSLGKFKGR 120
           + S+       P P L             ++L  +G F+GF+ T++       LGK KG 
Sbjct: 61  TTST-------PSPFLDFKSNKDTIANNNNTLQQQGCFVGFNTTEAKSHHVVPLGKLKGI 120

Query: 121 EFVSVFRFKTWWSTMWVGNSGSDLQMETQWFML--NVPEIKSYVVFIPIIEGSFRSAIHP 180
           +F S+FRFK WW+T WVG +G +LQ ETQ  +L  N+   + YV+ +PI+E SFR+++ P
Sbjct: 121 KFTSIFRFKVWWTTHWVGTNGHELQHETQILILDKNISLGRPYVLLLPILENSFRTSLQP 180

Query: 181 GVDGHVMICAESGSTHVKTSSFDAITYVHVSDNPYKLMKEAYAAIRVHMNTFRLLEEKPV 240
           G++ +V +  ESGSTHV  S+F A  Y+H+S++PY+L+KEA   I+  + TF+ LEEK  
Sbjct: 181 GLNDYVDMSVESGSTHVTGSTFKACLYLHLSNDPYRLVKEAVKVIQTKLGTFKTLEEKTP 240

Query: 241 THLLDKFGWCTWDAFYLTVDPVGIWNGVNDFAEGGVSPRFLIIDDGWQSINIDGEDPT-- 300
             +++KFGWCTWDAFYL V P G+W GV    +GG  P F+IIDDGWQSI+ D +DP   
Sbjct: 241 PSIIEKFGWCTWDAFYLKVHPKGVWEGVKALTDGGCPPGFVIIDDGWQSISHDDDDPVTE 300

Query: 301 RDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLSGPNAPSFDPKKPKLLIAKAIEIEHA 360
           RD  N    G QM  RL +++E  KFR+Y+ G                            
Sbjct: 301 RDGMNRTSAGEQMPCRLIKYEENYKFREYENGD--------------------------- 360

Query: 361 EKDRDKAIGSGVTDITKFETKIHKFKEELNAIFGKEQDSGCSSCSCKGDDSGMKAFTRDL 420
                                                          G   G+  F RDL
Sbjct: 361 ----------------------------------------------NGGKKGLVGFVRDL 420

Query: 421 RTKFKGLDDVFVWHALAGAWGGVRPGATHL-NSKIVPCKLSPGLDGTMTDLAVVKIIEGS 480
           + +F+ ++ V+VWHAL G WGGVRP    +  +K+V  KLSPG+  TM DLAV KI+E  
Sbjct: 421 KEEFRSVESVYVWHALCGYWGGVRPKVCGMPEAKVVVPKLSPGVKMTMEDLAVDKIVENG 480

Query: 481 IGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTKS 540
           +GLV P+ A + FD +HS+L   GI GVKVDV+H LE +SEEYGGRV+LAKAYYK LT S
Sbjct: 481 VGLVPPNLAQEMFDGIHSHLESAGIDGVKVDVIHLLELLSEEYGGRVELAKAYYKALTSS 540

Query: 541 LHKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHC 600
           ++K+FKG G+ +SM+ CNDFF LGT+  S+GRVGDDFW  DP+GDP G YWLQG HM+HC
Sbjct: 541 VNKHFKGNGVIASMEHCNDFFLLGTEAISLGRVGDDFWCCDPSGDPNGTYWLQGCHMVHC 600

Query: 601 AYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPD 660
           AYNS+WMG  I PDWDMFQS H CA+FHA SRAI GGPVYVSD VG HNF L+K  V PD
Sbjct: 601 AYNSLWMGNFIHPDWDMFQSTHPCAEFHAASRAISGGPVYVSDCVGNHNFKLLKSFVLPD 660

Query: 661 GTIPRCQHFALPTRDCLFKNPLFDSKTVLKIWNFNKYGGVIGAFNCQGAGWDPKEQRIKG 720
           G+I RCQH+ALPTRDCLF++PL + KT+LKIWN NKY GV+G FNCQG GW P+ +R K 
Sbjct: 661 GSILRCQHYALPTRDCLFEDPLHNGKTMLKIWNLNKYAGVLGLFNCQGGGWCPETRRNKS 720

Query: 721 HPECYKPMSTTVHVSDVEWDQKPEAAPMGNFTEYIVYLNQAEQILHTTPKFEPLKVTIQP 780
             E    ++      D+EW        +     + VY  + E+ L      + L+V+++P
Sbjct: 721 ASEFSHAVTCYASPEDIEWCNGKTPMDIKGVDVFAVYFFK-EKKLSLMKCSDRLEVSLEP 780

Query: 781 STFEIFSFIPLRKLNSN-IKFAPIGLKEMFNSSGTIQHLKYNENG--VEMKVKGGGNFIA 840
            +FE+ +  PL+  +   I+FAPIGL  M NS G +Q L+++++   V++ V+G G    
Sbjct: 781 FSFELMTVSPLKVFSKRLIQFAPIGLVNMLNSGGAVQSLEFDDSASLVKIGVRGCGELSV 798

Query: 841 YSSASPKKCISNGVEVEFEWDDSDGKLSFDLPWIEEAKGVSNVEILF 859
           ++S  P  C  +GV VEF+++D   ++    P    +  +S VE LF
Sbjct: 841 FASEKPVCCKIDGVSVEFDYEDKMVRVQILWP---GSSTLSLVEFLF 798

BLAST of CmaCh08G004780 vs. ExPASy Swiss-Prot
Match: Q5VQG4 (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=RFS PE=1 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 8.5e-202
Identity = 363/846 (42.91%), Postives = 491/846 (58.04%), Query Frame = 0

Query: 31  LSVKGVSLLSDVPSNVFFSSFSSICQPSDAPLPLLQRVHSLSHKGGFLGFDQTKSSDRLT 90
           L+V G   L DVP+N+  +  S++   SD P          +  G FLGFD   + DR  
Sbjct: 35  LAVDGHPFLLDVPANIRLTPASTLVPNSDVP---------AAAAGSFLGFDAPAAKDRHV 94

Query: 91  NSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWFMLNVPEIKS-------YVVF 150
             +GK +   F+S+FRFK WW+T WVG +G D++ ETQ  +L+    KS       YV+ 
Sbjct: 95  VPIGKLRDTRFMSIFRFKVWWTTHWVGTNGRDVENETQMMILDQSGTKSSPTGPRPYVLL 154

Query: 151 IPIIEGSFRSAIHPG-VDGHVMICAESGSTHVKTSSFDAITYVHVSDNPYKLMKEAYAAI 210
           +PI+EG FR+ +  G  + +V +  ESGS+ V+ S F +  Y+H  D+P+ L+K+A   +
Sbjct: 155 LPIVEGPFRACLESGKAEDYVHMVLESGSSTVRGSVFRSAVYLHAGDDPFDLVKDAMRVV 214

Query: 211 RVHMNTFRLLEEKPVTHLLDKFGWCTWDAFYLTVDPVGIWNGVNDFAEGGVSPRFLIIDD 270
           R H+ TFRL+EEK    ++DKFGWCTWDAFYL V P G+W GV   A+GG  P  ++IDD
Sbjct: 215 RAHLGTFRLMEEKTPPPIVDKFGWCTWDAFYLKVHPEGVWEGVRRLADGGCPPGLVLIDD 274

Query: 271 GWQSINIDGEDPTRDAK--NLVLGGTQMTARLYRFDECEKFRKYKGGSLSGPNAPSFDPK 330
           GWQSI  D +D    A+  N    G QM  RL +F E  KFR+YKG              
Sbjct: 275 GWQSICHDDDDLGSGAEGMNRTSAGEQMPCRLIKFQENYKFREYKG-------------- 334

Query: 331 KPKLLIAKAIEIEHAEKDRDKAIGSGVTDITKFETKIHKFKEELNAIFGKEQDSGCSSCS 390
                                                                       
Sbjct: 335 ------------------------------------------------------------ 394

Query: 391 CKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAGAWGGVRPGATHL-NSKIVPCKLSPGLD 450
                 GM  F R+++  F  ++ V+VWHAL G WGG+RPGA  L  +K+V  +LSPGL 
Sbjct: 395 ------GMGGFVREMKAAFPTVEQVYVWHALCGYWGGLRPGAPGLPPAKVVAPRLSPGLQ 454

Query: 451 GTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHTLEYVSEEYGG 510
            TM DLAV KI+   +GLV P +A + ++ +HS+L   GI GVKVDV+H LE V EEYGG
Sbjct: 455 RTMEDLAVDKIVNNGVGLVDPRRARELYEGLHSHLQASGIDGVKVDVIHLLEMVCEEYGG 514

Query: 511 RVDLAKAYYKGLTKSLHKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVGDDFWFQDPNGD 570
           RV+LAKAY+ GLT+S+ ++F G G+ +SM+ CNDF  LGT+  ++GRVGDDFW  DP+GD
Sbjct: 515 RVELAKAYFAGLTESVRRHFNGNGVIASMEHCNDFMLLGTEAVALGRVGDDFWCTDPSGD 574

Query: 571 PMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSV 630
           P G +WLQG HM+HCAYNS+WMG  I PDWDMFQS H CA FHA SRA+ GGPVYVSD+V
Sbjct: 575 PDGTFWLQGCHMVHCAYNSLWMGAFIHPDWDMFQSTHPCAAFHAASRAVSGGPVYVSDAV 634

Query: 631 GGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDSKTVLKIWNFNKYGGVIGAFN 690
           G H+FDL+++L  PDGTI RC+ +ALPTRDCLF +PL D KT+LKIWN NK+ GV+GAFN
Sbjct: 635 GCHDFDLLRRLALPDGTILRCERYALPTRDCLFADPLHDGKTMLKIWNVNKFSGVLGAFN 694

Query: 691 CQGAGWDPKEQRIKGHPECYKPMSTTVHVSDVEWDQKPEAAPMGNFTEYIVYLNQAEQIL 750
           CQG GW  + +R         P++     +DVEW         G    + VY  +A + L
Sbjct: 695 CQGGGWSREARRNMCAAGFSVPVTARASPADVEWSHGG-----GGGDRFAVYFVEARK-L 754

Query: 751 HTTPKFEPLKVTIQPSTFEIFSFIPLRKLNS---NIKFAPIGLKEMFNSSGTIQHL---- 810
               + E +++T++P T+E+    P+R + S    I FAPIGL  M N+ G +Q      
Sbjct: 755 QLLRRDESVELTLEPFTYELLVVAPVRAIVSPELGIGFAPIGLANMLNAGGAVQGFEAAR 783

Query: 811 KYNENGVEMKVKGGGNFIAYSSASPKKCISNGVEVEFEWDDSDGKLSFDLPWIEEAKGVS 859
           K  +   E+ VKG G  +AYSSA P+ C  NG + EF+++  DG ++ D+PW   +K +S
Sbjct: 815 KDGDVAAEVAVKGAGEMVAYSSARPRLCKVNGQDAEFKYE--DGIVTVDVPWTGSSKKLS 783

BLAST of CmaCh08G004780 vs. TAIR 10
Match: AT4G01970.1 (stachyose synthase )

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 514/847 (60.68%), Postives = 645/847 (76.15%), Query Frame = 0

Query: 22  NLVDFSDGKLSVK-GVSLLSDVPSNVFFSSFSSICQPSDAPLPLLQRVHSLSHKGGFLGF 81
           N  + S+G L  K    +L DVP NV F+ FSS    +DAPLP+L RV + +HKGGFLGF
Sbjct: 32  NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 82  DQTKSSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWFMLNVPEIKSY 141
            +   SDRLTNSLG+F+ REF+S+FRFK WWST W+G SGSDLQ ETQW ML +PEI SY
Sbjct: 92  TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEIDSY 151

Query: 142 VVFIPIIEGSFRSAIHPGVDGHVMICAESGSTHVKTSSFDAITYVHVSDNPYKLMKEAYA 201
           V  IP IEG+FR+++ PG  G+V+ICAESGST VK SSF +I Y+H+ DNPY LMKEA++
Sbjct: 152 VAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFS 211

Query: 202 AIRVHMNTFRLLEEKPVTHLLDKFGWCTWDAFYLTVDPVGIWNGVNDFAEGGVSPRFLII 261
           A+RVHMNTF+LLEEK +  ++DKFGWCTWDA YLTVDP  IW GV +F +GGV P+F+II
Sbjct: 212 ALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVII 271

Query: 262 DDGWQSINIDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLSGPNAPSFDPK 321
           DDGWQSIN DG++  +DA+NLVLGG QMTARL  F EC+KFR YKGGS    +A  F+P 
Sbjct: 272 DDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPL 331

Query: 322 KPKLLIAKAIEIEHAEKDRDKAI-GSGVTDITKFETKIHKFKEELNAIFGK-EQDSGCSS 381
           KPK+LI KA E   A   R K +  SG  D+T+ + KI    EELNA+F + E++    S
Sbjct: 332 KPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLGS 391

Query: 382 CSCKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAGAWGGVRPGA-THLNSKIVPCKLSPG 441
               G  SGM AFT+DLR +FK LDD++VWHAL GAW GVRP     L +K+ P +LSP 
Sbjct: 392 DDVSG--SGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAPFELSPS 451

Query: 442 LDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHTLEYVSEEY 501
           L  TM DLAV K++E  IGLVHP +A +F+DSMHSYL+ VG+TG K+DV  TLE ++EE+
Sbjct: 452 LGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQTLESLAEEH 511

Query: 502 GGRVDLAKAYYKGLTKSLHKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVGDDFWFQDPN 561
           GGRV+LAKAYY GLT+S+ KNF GT + +SMQQCN+FFFL TKQ SIGRVGDDFW+QDP 
Sbjct: 512 GGRVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGDDFWWQDPY 571

Query: 562 GDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSD 621
           GDP GVYWLQGVHMIHC+YNS+WMGQ+IQPDWDMFQSDH+CA++HA SRAICGGPVY+SD
Sbjct: 572 GDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAICGGPVYLSD 631

Query: 622 SVG--GHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDSKTVLKIWNFNKYGGVI 681
            +G   HNFDLIK+L + DGTIPRC H+ALPTRD LFKNPLFD +++LKI+NFNK+GGVI
Sbjct: 632 HLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFNFNKFGGVI 691

Query: 682 GAFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVSDVEWDQKPEAA-PMGNFT-EYIVYLN 741
           G FNCQGAGW P+E R KG+ ECY  +S TVHVSD+EWDQ PEAA     +T +Y+VY  
Sbjct: 692 GTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAAGSQVTYTGDYLVYKQ 751

Query: 742 QAEQILHTTPKFEPLKVTIQPSTFEIFSFIPLRKL-NSNIKFAPIGLKEMFNSSGTIQHL 801
           Q+E+IL    K E +K+T++PS F++ SF+P+ +L +S ++FAP+GL  MFN  GT+Q +
Sbjct: 752 QSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFAPLGLINMFNCVGTVQDM 811

Query: 802 KY-NENGVEMKVKGGGNFIAYSSASPKKCISNGVEVEFEWDDSDGKLSFDLPWIEEAKGV 859
           K   +N + + VKG G F+AYSS++P KC  N  E EF+W++  GKLSF +PW+EE+ G+
Sbjct: 812 KVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEETGKLSFFVPWVEESGGI 871

BLAST of CmaCh08G004780 vs. TAIR 10
Match: AT5G40390.1 (Raffinose synthase family protein )

HSP 1 Score: 741.9 bits (1914), Expect = 5.8e-214
Identity = 382/861 (44.37%), Postives = 516/861 (59.93%), Query Frame = 0

Query: 16  KSEGLDNLVDFS------DGKLSVKGVSLLSDVPSNVFFSSFSSICQPSDAPLPLLQRVH 75
           KS+   N VDF+      D  L   G  +L+DVP NV  +S   +      PL +     
Sbjct: 8   KSDSGINGVDFTEKFRLEDSTLLANGQVVLTDVPVNVTLTSSPYLVDKDGVPLDV----- 67

Query: 76  SLSHKGGFLGFD-QTKSSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQ 135
                G F+GF+   +       S+GK K   F+S+FRFK WW+T WVG++G D++ ETQ
Sbjct: 68  ---SAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQ 127

Query: 136 WFMLNVPEIKS---------YVVFIPIIEGSFRSAIHPGVDGHVMICAESGSTHVKTSSF 195
             +L+     S         YV+ +P++EGSFRS+   G D  V +C ESGST V  S F
Sbjct: 128 IIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEF 187

Query: 196 DAITYVHVSDNPYKLMKEAYAAIRVHMNTFRLLEEKPVTHLLDKFGWCTWDAFYLTVDPV 255
             I YVH  D+P+KL+K+A   IRVHMNTF+LLEEK    ++DKFGWCTWDAFYLTV+P 
Sbjct: 188 RQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPD 247

Query: 256 GIWNGVNDFAEGGVSPRFLIIDDGWQSINIDGEDPTRDAKNLVLGGTQMTARLYRFDECE 315
           G+  GV    +GG  P  ++IDDGWQSI  D +    +  N+ + G QM  RL +F+E  
Sbjct: 248 GVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENH 307

Query: 316 KFRKYKGGSLSGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDITKFETKIHK 375
           KF+ Y                                                       
Sbjct: 308 KFKDY------------------------------------------------------- 367

Query: 376 FKEELNAIFGKEQDSGCSSCSCKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAGAWGGVR 435
                  +  K+Q           +D GMKAF RDL+ +F  +D ++VWHAL G WGG+R
Sbjct: 368 -------VSPKDQ-----------NDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLR 427

Query: 436 PGATHL-NSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVG 495
           P A  L  S I+  +LSPGL  TM DLAV KIIE  IG   PD A +F++ +HS+L   G
Sbjct: 428 PEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAG 487

Query: 496 ITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTKSLHKNFKGTGLFSSMQQCNDFFFLG 555
           I GVKVDV+H LE + ++YGGRVDLAKAY+K LT S++K+F G G+ +SM+ CNDF FLG
Sbjct: 488 IDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLG 547

Query: 556 TKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLC 615
           T+  S+GRVGDDFW  DP+GDP G +WLQG HM+HCAYNS+WMG  IQPDWDMFQS H C
Sbjct: 548 TEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPC 607

Query: 616 AKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFD 675
           A+FHA SRAI GGP+Y+SD VG H+FDL+K+LV P+G+I RC+++ALPTRD LF++PL D
Sbjct: 608 AEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHD 667

Query: 676 SKTVLKIWNFNKYGGVIGAFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVSDVEWDQKPE 735
            KT+LKIWN NKY GVIGAFNCQG GW  + +R +   EC   ++ T    DVEW+    
Sbjct: 668 GKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSS 727

Query: 736 AAPMGNFTEYIVYLNQAEQILHTTPKFEPLKVTIQPSTFEIFSFIPLRKLNSN-IKFAPI 795
              + N  E+ ++L+Q++++L  +   + L++T++P  FE+ +  P+  +  N ++FAPI
Sbjct: 728 PISIANVEEFALFLSQSKKLL-LSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPI 783

Query: 796 GLKEMFNSSGTIQHLKYNENGVEMKVKGGGNFIAYSSASPKKCISNGVEVEFEWDDSDGK 855
           GL  M N+SG I+ L YN+  VE+ V G G F  Y+S  P  C+ +G  VEF ++DS   
Sbjct: 788 GLVNMLNTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYEDS--M 783

Query: 856 LSFDLPWIEEAKGVSNVEILF 859
           +   +PW     G+S+++ LF
Sbjct: 848 VMVQVPW-SGPDGLSSIQYLF 783

BLAST of CmaCh08G004780 vs. TAIR 10
Match: AT5G20250.1 (Raffinose synthase family protein )

HSP 1 Score: 476.9 bits (1226), Expect = 3.5e-134
Identity = 284/836 (33.97%), Postives = 410/836 (49.04%), Query Frame = 0

Query: 24  VDFSDGKLSVKGVSLLSDVPSNVFFSSFSSICQPSDAPLPLLQRVHSLSHKGGFLGFDQT 83
           V  SDG L +K  ++L+ VP NV  +S S        P+           +G F+G    
Sbjct: 7   VRISDGNLIIKNRTILTGVPDNVITTSASEA-----GPV-----------EGVFVGAVFN 66

Query: 84  KSSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWFMLNVPE------- 143
           K   +    +G  +   F+S FRFK WW    +G  G D+  ETQ+ ++   +       
Sbjct: 67  KEESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESD 126

Query: 144 --------IKSYVVFIPIIEGSFRSAIHPGVDGHVMICAESGSTHVKTSSFDAITYVHVS 203
                    K Y VF+P+IEGSFRS +   V+  V +C ESG    K SSF    Y+H  
Sbjct: 127 GANGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYIHAG 186

Query: 204 DNPYKLMKEAYAAIRVHMNTFRLLEEKPVTHLLDKFGWCTWDAFYLTVDPVGIWNGVNDF 263
            +P++ + +A   +++H+N+FR   EK +  ++D FGWCTWDAFY  V   G+  G+   
Sbjct: 187 TDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSL 246

Query: 264 AEGGVSPRFLIIDDGWQSINIDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGS 323
           A GG  P+F+IIDDGWQS+  D      D K           RL    E EKF+K     
Sbjct: 247 AAGGTPPKFVIIDDGWQSVERDATVEAGDEKK-----ESPIFRLTGIKENEKFKK----- 306

Query: 324 LSGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDITKFETKIHKFKEELNAIF 383
              PN                                G+ +I K   + H          
Sbjct: 307 KDDPNV-------------------------------GIKNIVKIAKEKH---------- 366

Query: 384 GKEQDSGCSSCSCKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAGAWGGVRPGATHLNSK 443
                                           GL  V+VWHA+ G WGGVRPG  + +  
Sbjct: 367 --------------------------------GLKYVYVWHAITGYWGGVRPGEEYGSVM 426

Query: 444 IVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMH 503
             P      ++   T    V  ++G +GLV P +   F++ +HSYL+  G+ GVKVDV  
Sbjct: 427 KYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQC 486

Query: 504 TLEYVSEEYGGRVDLAKAYYKGLTKSLHKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVG 563
            LE +    GGRV+L + +++ L  S+ KNF   G  + M    D  +  +KQ ++ R  
Sbjct: 487 VLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC-SKQAAVIRAS 546

Query: 564 DDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAI 623
           DDF+ +DP            +H+   AYNS+++G+ +QPDWDMF S H  A++HA +RAI
Sbjct: 547 DDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAI 606

Query: 624 CGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDSKTVLKIWNF 683
            GGP+YVSDS G HNF+L+++LV PDG+I R +    PTRDCLF +P  D  ++LKIWN 
Sbjct: 607 SGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDGVSLLKIWNM 666

Query: 684 NKYGGVIGAFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVSDVEWDQKPEAAPMGNFTEY 743
           NKY GV+G +NCQGA W   E++   H      ++ ++   DV    +    P     + 
Sbjct: 667 NKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDC 726

Query: 744 IVYLNQAEQILHTTPKFEPLKVTIQPSTFEIFSFIPLRKLNSNIKFAPIGLKEMFNSSGT 803
            VY +Q+   L   P    L V+++    EIF+  P+  L   + FAPIGL  M+NS G 
Sbjct: 727 AVY-SQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIGLVNMYNSGGA 732

Query: 804 IQHLKY--NENGVEMKVKGGGNFIAYSSASPKKCISNGVEVEFEWDDSDGKLSFDL 843
           I+ L+Y   +  V M+VKG G F +YSS  PK+C+    E+ FE+D S G ++F+L
Sbjct: 787 IEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSGLVTFEL 732

BLAST of CmaCh08G004780 vs. TAIR 10
Match: AT5G20250.2 (Raffinose synthase family protein )

HSP 1 Score: 476.9 bits (1226), Expect = 3.5e-134
Identity = 284/836 (33.97%), Postives = 410/836 (49.04%), Query Frame = 0

Query: 24  VDFSDGKLSVKGVSLLSDVPSNVFFSSFSSICQPSDAPLPLLQRVHSLSHKGGFLGFDQT 83
           V  SDG L +K  ++L+ VP NV  +S S        P+           +G F+G    
Sbjct: 7   VRISDGNLIIKNRTILTGVPDNVITTSASEA-----GPV-----------EGVFVGAVFN 66

Query: 84  KSSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWFMLNVPE------- 143
           K   +    +G  +   F+S FRFK WW    +G  G D+  ETQ+ ++   +       
Sbjct: 67  KEESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESD 126

Query: 144 --------IKSYVVFIPIIEGSFRSAIHPGVDGHVMICAESGSTHVKTSSFDAITYVHVS 203
                    K Y VF+P+IEGSFRS +   V+  V +C ESG    K SSF    Y+H  
Sbjct: 127 GANGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYIHAG 186

Query: 204 DNPYKLMKEAYAAIRVHMNTFRLLEEKPVTHLLDKFGWCTWDAFYLTVDPVGIWNGVNDF 263
            +P++ + +A   +++H+N+FR   EK +  ++D FGWCTWDAFY  V   G+  G+   
Sbjct: 187 TDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSL 246

Query: 264 AEGGVSPRFLIIDDGWQSINIDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGS 323
           A GG  P+F+IIDDGWQS+  D      D K           RL    E EKF+K     
Sbjct: 247 AAGGTPPKFVIIDDGWQSVERDATVEAGDEKK-----ESPIFRLTGIKENEKFKK----- 306

Query: 324 LSGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDITKFETKIHKFKEELNAIF 383
              PN                                G+ +I K   + H          
Sbjct: 307 KDDPNV-------------------------------GIKNIVKIAKEKH---------- 366

Query: 384 GKEQDSGCSSCSCKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAGAWGGVRPGATHLNSK 443
                                           GL  V+VWHA+ G WGGVRPG  + +  
Sbjct: 367 --------------------------------GLKYVYVWHAITGYWGGVRPGEEYGSVM 426

Query: 444 IVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMH 503
             P      ++   T    V  ++G +GLV P +   F++ +HSYL+  G+ GVKVDV  
Sbjct: 427 KYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQC 486

Query: 504 TLEYVSEEYGGRVDLAKAYYKGLTKSLHKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVG 563
            LE +    GGRV+L + +++ L  S+ KNF   G  + M    D  +  +KQ ++ R  
Sbjct: 487 VLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC-SKQAAVIRAS 546

Query: 564 DDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAI 623
           DDF+ +DP            +H+   AYNS+++G+ +QPDWDMF S H  A++HA +RAI
Sbjct: 547 DDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAI 606

Query: 624 CGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDSKTVLKIWNF 683
            GGP+YVSDS G HNF+L+++LV PDG+I R +    PTRDCLF +P  D  ++LKIWN 
Sbjct: 607 SGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDGVSLLKIWNM 666

Query: 684 NKYGGVIGAFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVSDVEWDQKPEAAPMGNFTEY 743
           NKY GV+G +NCQGA W   E++   H      ++ ++   DV    +    P     + 
Sbjct: 667 NKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDC 726

Query: 744 IVYLNQAEQILHTTPKFEPLKVTIQPSTFEIFSFIPLRKLNSNIKFAPIGLKEMFNSSGT 803
            VY +Q+   L   P    L V+++    EIF+  P+  L   + FAPIGL  M+NS G 
Sbjct: 727 AVY-SQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIGLVNMYNSGGA 732

Query: 804 IQHLKY--NENGVEMKVKGGGNFIAYSSASPKKCISNGVEVEFEWDDSDGKLSFDL 843
           I+ L+Y   +  V M+VKG G F +YSS  PK+C+    E+ FE+D S G ++F+L
Sbjct: 787 IEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSGLVTFEL 732

BLAST of CmaCh08G004780 vs. TAIR 10
Match: AT5G20250.3 (Raffinose synthase family protein )

HSP 1 Score: 476.9 bits (1226), Expect = 3.5e-134
Identity = 284/836 (33.97%), Postives = 410/836 (49.04%), Query Frame = 0

Query: 24  VDFSDGKLSVKGVSLLSDVPSNVFFSSFSSICQPSDAPLPLLQRVHSLSHKGGFLGFDQT 83
           V  SDG L +K  ++L+ VP NV  +S S        P+           +G F+G    
Sbjct: 7   VRISDGNLIIKNRTILTGVPDNVITTSASEA-----GPV-----------EGVFVGAVFN 66

Query: 84  KSSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWFMLNVPE------- 143
           K   +    +G  +   F+S FRFK WW    +G  G D+  ETQ+ ++   +       
Sbjct: 67  KEESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESD 126

Query: 144 --------IKSYVVFIPIIEGSFRSAIHPGVDGHVMICAESGSTHVKTSSFDAITYVHVS 203
                    K Y VF+P+IEGSFRS +   V+  V +C ESG    K SSF    Y+H  
Sbjct: 127 GANGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYIHAG 186

Query: 204 DNPYKLMKEAYAAIRVHMNTFRLLEEKPVTHLLDKFGWCTWDAFYLTVDPVGIWNGVNDF 263
            +P++ + +A   +++H+N+FR   EK +  ++D FGWCTWDAFY  V   G+  G+   
Sbjct: 187 TDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSL 246

Query: 264 AEGGVSPRFLIIDDGWQSINIDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGS 323
           A GG  P+F+IIDDGWQS+  D      D K           RL    E EKF+K     
Sbjct: 247 AAGGTPPKFVIIDDGWQSVERDATVEAGDEKK-----ESPIFRLTGIKENEKFKK----- 306

Query: 324 LSGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDITKFETKIHKFKEELNAIF 383
              PN                                G+ +I K   + H          
Sbjct: 307 KDDPNV-------------------------------GIKNIVKIAKEKH---------- 366

Query: 384 GKEQDSGCSSCSCKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAGAWGGVRPGATHLNSK 443
                                           GL  V+VWHA+ G WGGVRPG  + +  
Sbjct: 367 --------------------------------GLKYVYVWHAITGYWGGVRPGEEYGSVM 426

Query: 444 IVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMH 503
             P      ++   T    V  ++G +GLV P +   F++ +HSYL+  G+ GVKVDV  
Sbjct: 427 KYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQC 486

Query: 504 TLEYVSEEYGGRVDLAKAYYKGLTKSLHKNFKGTGLFSSMQQCNDFFFLGTKQNSIGRVG 563
            LE +    GGRV+L + +++ L  S+ KNF   G  + M    D  +  +KQ ++ R  
Sbjct: 487 VLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC-SKQAAVIRAS 546

Query: 564 DDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAI 623
           DDF+ +DP            +H+   AYNS+++G+ +QPDWDMF S H  A++HA +RAI
Sbjct: 547 DDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAI 606

Query: 624 CGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDSKTVLKIWNF 683
            GGP+YVSDS G HNF+L+++LV PDG+I R +    PTRDCLF +P  D  ++LKIWN 
Sbjct: 607 SGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDGVSLLKIWNM 666

Query: 684 NKYGGVIGAFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVSDVEWDQKPEAAPMGNFTEY 743
           NKY GV+G +NCQGA W   E++   H      ++ ++   DV    +    P     + 
Sbjct: 667 NKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDC 726

Query: 744 IVYLNQAEQILHTTPKFEPLKVTIQPSTFEIFSFIPLRKLNSNIKFAPIGLKEMFNSSGT 803
            VY +Q+   L   P    L V+++    EIF+  P+  L   + FAPIGL  M+NS G 
Sbjct: 727 AVY-SQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIGLVNMYNSGGA 732

Query: 804 IQHLKY--NENGVEMKVKGGGNFIAYSSASPKKCISNGVEVEFEWDDSDGKLSFDL 843
           I+ L+Y   +  V M+VKG G F +YSS  PK+C+    E+ FE+D S G ++F+L
Sbjct: 787 IEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSGLVTFEL 732