CmaCh08G003610 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh08G003610
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	HMA domain-containing protein
Location	Cma_Chr08: 2078471 .. 2081507 (-)
RNA-Seq Expression	CmaCh08G003610
Synteny	CmaCh08G003610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCTAGCCACGCATCCAGTGATCGAGTATGATAATGTTCTTCCTTCTTCTTTTTATTTTTTTATTTTTTTATTTTTTTATTTATTTATTTATTTATTTTGATCATATCGTTCATCTTTTTGTTTAGACTTATGTTCTCAAAGTGAATCTCAAGTGTTGCAACGCATGCCCCAACAGAGTCCAGAAGAACCTTCTCAAACTTCCAGGTATGCCCATATAGCTTCACTCATAAATTTAAATTTAAAAATTATTTTTTAATTTTGACACGGATCGACTATTCACTTTAAATTTTAGTATTTTCTAGAATATACAAATTTATCAAACACAAAATCGAACATTGAAATCATTATAAAAGATACAACATGTGGGATTTCACATTGGTTGAGTAATAGGGAGGACGTTCGGCCTCAAAGAGAGTGTGGATTGTGAGATCTCACATCGGTTGAGTGTCAACAATGACGTTGGCCCAAAAGAGGGCGTAAATTGTGAGATTCCACATCAGTTGAGTGCCAACGAGGTCGCTGACCCCAAAAGGGGGTGGAGTGTGAGATTTCACATCGTTTGAGTGCTAGAGAGGACAATGGGTCCCAAAGAGGGTGGAGTGTGAGATCGTTGAGTGCCAACGAGGATGCTAGTCTAAAAGAAGGGTGGATTGTGAGATCTCACCTCGGTTGAGTGCCAGTGAGGACAATGGCCCCAAAGAGAGGTGGAGTGTGAGATTACATGTAGGTTGGAAAGGAGAACTAAACATACTTGCAAGAGTGTGAAAATCTCTCACGAGGTTTAAACAAACAAAATTTGTTAAAAACATTTATAATTTATGGATGTTTTGTCTGTTTGTTTTTGGTTGTAATGGCGGCCAATGAAGATGTCAAATCCGTAGCCATTGATATACAAAAAGGATTGGTAAGTATCACTGCAAATTACAGCTTTGATCCATTAACCATCGTACGTGCAATGTCAAAAACTGGTAAGCTAGCACGATTACAACCCATGGGATATACGGCGCCGGCGACCTCCGCTGCTGTCAGAGATTTCGTTCATCAGAATATTCCTTCTGCTCCTTCAGATCCTCACTGTAACAAAATTGATTGGACCCAGAACTGCGTTTCTGGCTCCAGCAAGGTTCATTTCAACGAGGTGGCCGGCGCCGTTCCGCCGGTGGATGTGCCGTACCGCCACCGGCTGTCTACTGGGCTGGGGTCGCAATTTGGAGGTGTGGATCATGATGCAAGCCGTGGGTTTGCGCGTCCAATGATGGAGCCGTTGCCACAGCCTCTGCCGCCAGCCATGCCACCAGCCATGCCACCAGCCATGCCACCAGGGGACGGATATTATCGATGGTCTGAGCCGCCACCTTATTATTGGCAGCAGCAACCGCCCCCCGCTGCTGCACCGGGGCACTACTTGTTCAATGATGAGAATGTAGAGGGGTGTAAGATCATTTAGACCGAGTTATATTTATATTGGCTTTACTAACCTTTTTTTGATTCATGTAAGAATACGAGCGATAAACCAAATGAATCCAATAGTTTCATGATGCCGATCTAAATTTGTTATTATTCTCAGCACTTTGTCTATAGATTAAATGGTTATATTCATTAAATTTAAAATGAGAAATTGATATGAACTTAGAGACATCATTCATATAATATACTTCAAAATACCTTCAATTTTGTTTGGATTACTAGAATTATTGTATTTCATTAATATATTTTAAGATTTTTTATTTACTCGCAAGTTATATTTACATAATCAGACATAAAATATAAATGTTAAAACTCTTGAAATGTTTTTAATATTTTCATTTGATATTATATAAAGTTATTATATTATATTTGTAAGTAGACTTGAAAAATATAGTAAGAGAAGCATTTCATTAGCCAATGGCCTCTTTGCATATAGAATCATTCAAGCTCGACGTGATCAATCTTGCTTTATAAAATAAAGTAAGAGAAGCATTTCATTAGCCAATGGCCTCTATGCATATAGAATCATTCAAGCTCGACGTGATCAATCTTGCTTTATAAAATAAAGTAAGAGAAGCATTTCATTAGCCAATGGCCTCTATGCATATAGAATCATTCAAGCTCGACGTGATCAATCTTGCTTTATAAAATAAAGTAAGAGAAGCATTTCATTAGCCAATGGCCTCTATGCATATAGAATCATTCAAGCTCGACGTGATCAATCTTGCTTTATAAAATAAAGTAAGAGAAGCATTTCATTAGCCAATGGCCTCTATGCATATAGAATCATTCAAGCTCGACGTGATCAATCTTGCTTTATAAAATAAAGTAAGAGAAGCATTTCATTAGCCAATGGCCTCTATGCATATAGAATCATATAAGTTCGACGTGATCAATCTTGTTTATAGAGGGATTCAAATCTCAAACAAGTGTTCTTGCCTTAAATATTTGATCAACAAAAATGATATGATCGTTTTTGCTTGTAAGTGATTCAAATCTCAAATAAGGTATTCTTGTCTTGACATATTCGACAAGATAATTTGAATCTTACTCTACCTGTAATGATTTCATATCATAAATGATATACTTCATTCACTATATATAATTTTAAATCTTCAAAAATCAACTTTAATTTCATTGATTTTAATAAAATTAAAGGTGCACTAGAGATGATCTCAAATGTGAGGCATTTTTGTTGGAACAATTTTTGAAAAGTTATGATTCAAATAAAAATTAATTAGTCCTTAAATATGCTTGCTTGAATTCATTAAACAAAAAACTGTATTCAAAAGAGTTTATGGATCCTGTTTATCAAGTTTTGTGCTTGATTGAAAGTAGATCTACTTGGTTCTTTAAATTTTCAAAATGTCCCAAAATATAATTAACATGGAAGCTTGAAATTAACTTTAATTGGTCCTATAAAAAAATAAATAAACATACGTATGCATATACAAACTTCAAACTCGATTAAAAAATGCATAGCATAGCATAAAGTCATAGATAGTATACGTTAACTGTTACATTTATCATTCTAATAGGTCAATTAGACCAAAAAATCATTTAG

mRNA sequence

ATGGAAGCTAGCCACGCATCCAGTGATCGAACTTATGTTCTCAAAGTGAATCTCAAGTGTTGCAACGCATGCCCCAACAGAGTCCAGAAGAACCTTCTCAAACTTCCAGATGTCAAATCCGTAGCCATTGATATACAAAAAGGATTGGTAAGTATCACTGCAAATTACAGCTTTGATCCATTAACCATCGTACGTGCAATGTCAAAAACTGGTAAGCTAGCACGATTACAACCCATGGGATATACGGCGCCGGCGACCTCCGCTGCTGTCAGAGATTTCGTTCATCAGAATATTCCTTCTGCTCCTTCAGATCCTCACTGTAACAAAATTGATTGGACCCAGAACTGCGTTTCTGGCTCCAGCAAGGTTCATTTCAACGAGGTGGCCGGCGCCGTTCCGCCGGTGGATGTGCCGTACCGCCACCGGCTGTCTACTGGGCTGGGGTCGCAATTTGGAGGTGTGGATCATGATGCAAGCCGTGGGTTTGCGCGTCCAATGATGGAGCCGTTGCCACAGCCTCTGCCGCCAGCCATGCCACCAGCCATGCCACCAGCCATGCCACCAGGGGACGGATATTATCGATGGTCTGAGCCGCCACCTTATTATTGGCAGCAGCAACCGCCCCCCGCTGCTGCACCGGGGCACTACTTGTTCAATGATGAGAATGTAGAGGGGTGTCAATTAGACCAAAAAATCATTTAG

Coding sequence (CDS)

ATGGAAGCTAGCCACGCATCCAGTGATCGAACTTATGTTCTCAAAGTGAATCTCAAGTGTTGCAACGCATGCCCCAACAGAGTCCAGAAGAACCTTCTCAAACTTCCAGATGTCAAATCCGTAGCCATTGATATACAAAAAGGATTGGTAAGTATCACTGCAAATTACAGCTTTGATCCATTAACCATCGTACGTGCAATGTCAAAAACTGGTAAGCTAGCACGATTACAACCCATGGGATATACGGCGCCGGCGACCTCCGCTGCTGTCAGAGATTTCGTTCATCAGAATATTCCTTCTGCTCCTTCAGATCCTCACTGTAACAAAATTGATTGGACCCAGAACTGCGTTTCTGGCTCCAGCAAGGTTCATTTCAACGAGGTGGCCGGCGCCGTTCCGCCGGTGGATGTGCCGTACCGCCACCGGCTGTCTACTGGGCTGGGGTCGCAATTTGGAGGTGTGGATCATGATGCAAGCCGTGGGTTTGCGCGTCCAATGATGGAGCCGTTGCCACAGCCTCTGCCGCCAGCCATGCCACCAGCCATGCCACCAGCCATGCCACCAGGGGACGGATATTATCGATGGTCTGAGCCGCCACCTTATTATTGGCAGCAGCAACCGCCCCCCGCTGCTGCACCGGGGCACTACTTGTTCAATGATGAGAATGTAGAGGGGTGTCAATTAGACCAAAAAATCATTTAG

Protein sequence

MEASHASSDRTYVLKVNLKCCNACPNRVQKNLLKLPDVKSVAIDIQKGLVSITANYSFDPLTIVRAMSKTGKLARLQPMGYTAPATSAAVRDFVHQNIPSAPSDPHCNKIDWTQNCVSGSSKVHFNEVAGAVPPVDVPYRHRLSTGLGSQFGGVDHDASRGFARPMMEPLPQPLPPAMPPAMPPAMPPGDGYYRWSEPPPYYWQQQPPPAAAPGHYLFNDENVEGCQLDQKII

Homology

[Close]

BLAST of CmaCh08G003610 vs. ExPASy Swiss-Prot
Match: Q0WV37 (Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX=3702 GN=HIPP34 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 8.1e-06
Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 10 RTYVLKVNLKCCNACPNRVQKNLLKLPDVKSVAIDIQKGLVSITANYSFDPLTIVRAMSK 69
          +T VLKVN+  C  C ++V+K L K+  V SV  D+++G V++T N   DP  +V+ +SK
Sbjct: 10 QTCVLKVNVH-CEGCKHKVKKQLQKIEGVYSVKADVEQGRVTVTGN--IDPALLVKKLSK 69

Query: 70 TGKLARL 77
          +GK A +
Sbjct: 70 SGKHAEI 73

[Close]

BLAST of CmaCh08G003610 vs. TAIR 10
Match: AT4G23882.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 53.1 bits (126), Expect = 3.4e-07
Identity = 55/211 (26.07%), Postives = 101/211 (47.87%), Query Frame = 0


Query: 10  RTYVLKVNLKCCNACPNRVQKNLLKLPDVKSVAIDIQKGLVSITANYSFDPLTIVRAMSK 69
           RT VL+V +KCC  C  + ++ LL +  V +V  + ++GL+++T +   +P T++  ++K
Sbjct: 78  RTCVLRVGIKCCKGCQTKAKRKLLNVSGVSTVEYNAEQGLLTVTGD--ANPTTLLHKLTK 137

Query: 70  TGKLARLQPM---GYTA--PATSAAVRDFVHQNIPSAPSDPHCNKIDWTQNCVSGSSKVH 129
            GK A L       Y++  P T    ++   +     P+   C  +    N  S ++K+ 
Sbjct: 138 WGKKAELVSFLGDNYSSHVPRTPEQNQNKTMEKKKKKPT--KCCLLMCFGNKRSKNTKIE 197

Query: 130 FNEVAGAVPPVDVP-YRHRLSTGLGSQFGGVDHDASRGFARPMMEPLPQPLP------PA 189
                    P+ +P +++R   G+G++ G      +   + PM+ P PQ +P      P 
Sbjct: 198 ---------PMAIPNWQYR---GVGNENGNARPFINAAMSPPMVYPPPQAVPGFTTPIPY 257

Query: 190 MPPAMPPAMPP----GDGYYRWSEP--PPYY 203
            PP+  P  PP    G G ++ + P  PPY+
Sbjct: 258 PPPSFFPGRPPPPYTGAGMFQSAPPQSPPYF 272

[Close]

BLAST of CmaCh08G003610 vs. TAIR 10
Match: AT3G05220.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 5.8e-07
Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 10 RTYVLKVNLKCCNACPNRVQKNLLKLPDVKSVAIDIQKGLVSITANYSFDPLTIVRAMSK 69
          +T VLKVN+  C  C ++V+K L K+  V SV  D+++G V++T N   DP  +V+ +SK
Sbjct: 10 QTCVLKVNVH-CEGCKHKVKKQLQKIEGVYSVKADVEQGRVTVTGN--IDPALLVKKLSK 69

Query: 70 TGKLARL 77
          +GK A +
Sbjct: 70 SGKHAEI 73

[Close]

BLAST of CmaCh08G003610 vs. TAIR 10
Match: AT3G04900.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 44.3 bits (103), Expect = 1.6e-04
Identity = 52/223 (23.32%), Postives = 89/223 (39.91%), Query Frame = 0


Query: 13  VLKVNLKCCNACPNRVQKNLLKLPDVKSVAIDIQKGLVSITANYSFDPLTIVRAMSKTGK 72
           +LK+NL+CC   P+RV+K L ++  V ++ ID  KGL+ +    + +P  +++A++K G+
Sbjct: 10  ILKMNLQCCEDFPSRVKKLLRQVKGVYAITIDPVKGLILVCG--TAEPSVLIKAVAKLGQ 69

Query: 73  LARLQPMGYTAPATSAAVRDFVH-------QNIPSAPSDPHCNKIDWTQNCVSGSSKVHF 132
             +L              R  +        Q+ PS P+ P        + C +G      
Sbjct: 70  SPQLYAYEKDPATAKTRFRTLLKRYATNKTQDKPSPPAPP-VTATTPVETCPAGGETFRG 129

Query: 133 NEVAGAVPPVDVPYRHRLSTGLGSQFGGVDHDASRGFARPMMEPLPQPLPPAMPPAMPPA 192
               G    + +P            F         G+  P   P  +   P + P  PPA
Sbjct: 130 FGYPGPTTMMQMP-----------AFSLPPPRGLPGWLAPPTNPRLKYEEPKVTPRKPPA 189

Query: 193 MPPGDGYYRWSEPPPYYWQQQPPPAAAPGHYLFNDENVEGCQL 229
             P D          YY     PP+ +  +Y F+D+N + C +
Sbjct: 190 PYPFD----------YYENLGFPPSDSLFNY-FSDDNPQPCSI 207

The following BLAST results are available for this feature:

BLAST of CmaCh08G003610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WV37	8.1e-06	41.79	Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX...	[more]

BLAST of CmaCh08G003610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23882.1	3.4e-07	26.07	Heavy metal transport/detoxification superfamily protein	[more]
AT3G05220.1	5.8e-07	41.79	Heavy metal transport/detoxification superfamily protein	[more]
AT3G04900.1	1.6e-04	23.32	Heavy metal transport/detoxification superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 21..72 e-value: 2.6E-8 score: 34.2
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 11..76 score: 11.852175
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 21..75 e-value: 5.51412E-10 score: 51.8377
None	No IPR available	GENE3D	3.30.70.100		coord: 7..76 e-value: 4.8E-12 score: 48.1
None	No IPR available	PANTHER	PTHR45868:SF13	SUBFAMILY NOT NAMED	coord: 12..227
None	No IPR available	PANTHER	PTHR45868	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATED	coord: 12..227
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 7..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh08G003610.1	CmaCh08G003610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0046872	metal ion binding