CmaCh07G013100 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G013100
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpolygalacturonase inhibitor-like
LocationCma_Chr07: 7412092 .. 7413587 (-)
RNA-Seq ExpressionCmaCh07G013100
SyntenyCmaCh07G013100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGAAGCCCTTATCATCAACCATGCTTGTTCTCACCCTCAAATCTCCTCTTCCTCTCCTCTTCTTCTTCATCTTCTTCACCATTTCATTTGCAGAACTCTGCAACCCAAACGACAAGAAAGCTCTCCTAAACATCAAGAAAGCATTTAACAACGCGTACTACTTCGCCTCATGGAAGCCCGAAGAAGATTGTTGTACTTGGTATAGCGTCGAATGTGATGAGAATTCCCACCGGATTATCTCCCTCGACATCTCCACCGACAATGAAATCTCTGGCCAAATCCCGCCGTATGTCGGCGACCTCCCATTCCTCCAATCCCTCATGTTACACAAGCTCCCAAATCTCGTCGGCCCCATCCAACCCGCCATCGCCAAGCTCCACAACCTCAAACACCTCGACATCAGCTGGACCAATATCTCCGGCACCATCCCGGACTTCCTCAGCTCCCTCTCCAAGCTCACATACATTCGCCTCTCCTTCAGCAATCTCTCCGGAGCCATCCCGAGCTCCCTCTCCAAGCTCCCAAATCTTGAGTTTCTTCAATTGGACCGTAGCAAACTGACAGGACTAATCCCATATTCTTTCGGGGACTTTAAGGCTAAAGGTTTCTACCTTTATCTTTCCCACAACAAACTTTCCGGCAAAATCCCACCTTCCCTCTCTAGAGTGGACTTCGAATCCGTCGATTTGTCAAGAAACAAGCTTGAAGGCGATGCCTCCATGATATTCGGCGCACACAGGTGAGAATATTTACTAAAACTACTGCACCTAGATGATGGAGAAATTCTCTATTTATAATCATCAAATAAATGGACACATTAACTATAATAAACAGAAATATCATATCGAGATAGTTGCCTATAATTAAACGTCAAATATAATAGATATATGATAAACTATAATTCATGACTAAATATCTTTAACAACAGGAACACATGGAAAGTTGATTTGTCAAGGAATTTGTTGGAGTTTGACATGTCAAAGGTTGTGTTTTCAAGGAGCTTGTTTATGTTGGATCTTAACCATAATAAGATCTTTGGGGGTTTGCCGCCGCAGATGACGAAGCTCAGTAATCTTAACTCGTTCAATGTGAGTTACAATAGGCTTTGTGGTCAGATTCCAAAGGGTGGAGAGTTGCAGAGATTTGATGTTTATTCGTATTTTCATAATAAGTGTTTGTGTGGTGCACCACTTGAGAGCTGCAAGTGATGATCCACTTCTCATTATGCAGTGGAAATTTCAAGTTTCTATTAATAAAAGTGTTGTGTAAGGTTTCTATTAAGTATTTAATGTACTGATTGGTATACAAATTCTAGCTCTTCCTAAAGTTGGAGAATATTAAAGATGTTTCATTATGGTCAAAACGTGTAAGTTGCTAACACATACGACAAATGCACAATTTGATAAGGCAATTGAAATCCATGTTGGGAGATGAGACCTGCATCGGCTAATTAAGGGGTTAATTATGGGTTTATAGTAGGAATACATTTCCATTG

mRNA sequence

CTGAAGCCCTTATCATCAACCATGCTTGTTCTCACCCTCAAATCTCCTCTTCCTCTCCTCTTCTTCTTCATCTTCTTCACCATTTCATTTGCAGAACTCTGCAACCCAAACGACAAGAAAGCTCTCCTAAACATCAAGAAAGCATTTAACAACGCGTACTACTTCGCCTCATGGAAGCCCGAAGAAGATTGTTGTACTTGGTATAGCGTCGAATGTGATGAGAATTCCCACCGGATTATCTCCCTCGACATCTCCACCGACAATGAAATCTCTGGCCAAATCCCGCCGTATGTCGGCGACCTCCCATTCCTCCAATCCCTCATGTTACACAAGCTCCCAAATCTCGTCGGCCCCATCCAACCCGCCATCGCCAAGCTCCACAACCTCAAACACCTCGACATCAGCTGGACCAATATCTCCGGCACCATCCCGGACTTCCTCAGCTCCCTCTCCAAGCTCACATACATTCGCCTCTCCTTCAGCAATCTCTCCGGAGCCATCCCGAGCTCCCTCTCCAAGCTCCCAAATCTTGAGTTTCTTCAATTGGACCGTAGCAAACTGACAGGACTAATCCCATATTCTTTCGGGGACTTTAAGGCTAAAGGTTTCTACCTTTATCTTTCCCACAACAAACTTTCCGGCAAAATCCCACCTTCCCTCTCTAGAGTGGACTTCGAATCCGTCGATTTGTCAAGAAACAAGCTTGAAGGCGATGCCTCCATGATATTCGGCGCACACAGGAACACATGGAAAGTTGATTTGTCAAGGAATTTGTTGGAGTTTGACATGTCAAAGGTTGTGTTTTCAAGGAGCTTGTTTATGTTGGATCTTAACCATAATAAGATCTTTGGGGGTTTGCCGCCGCAGATGACGAAGCTCAGTAATCTTAACTCGTTCAATGTGAGTTACAATAGGCTTTGTGGTCAGATTCCAAAGGGTGGAGAGTTGCAGAGATTTGATGTTTATTCGTATTTTCATAATAAGTGTTTGTGTGGTGCACCACTTGAGAGCTGCAAGTGATGATCCACTTCTCATTATGCAGTGGAAATTTCAAGTTTCTATTAATAAAAGTGTTGTGTAAGGTTTCTATTAAGTATTTAATGTACTGATTGGTATACAAATTCTAGCTCTTCCTAAAGTTGGAGAATATTAAAGATGTTTCATTATGGTCAAAACGTGTAAGTTGCTAACACATACGACAAATGCACAATTTGATAAGGCAATTGAAATCCATGTTGGGAGATGAGACCTGCATCGGCTAATTAAGGGGTTAATTATGGGTTTATAGTAGGAATACATTTCCATTG

Coding sequence (CDS)

ATGCTTGTTCTCACCCTCAAATCTCCTCTTCCTCTCCTCTTCTTCTTCATCTTCTTCACCATTTCATTTGCAGAACTCTGCAACCCAAACGACAAGAAAGCTCTCCTAAACATCAAGAAAGCATTTAACAACGCGTACTACTTCGCCTCATGGAAGCCCGAAGAAGATTGTTGTACTTGGTATAGCGTCGAATGTGATGAGAATTCCCACCGGATTATCTCCCTCGACATCTCCACCGACAATGAAATCTCTGGCCAAATCCCGCCGTATGTCGGCGACCTCCCATTCCTCCAATCCCTCATGTTACACAAGCTCCCAAATCTCGTCGGCCCCATCCAACCCGCCATCGCCAAGCTCCACAACCTCAAACACCTCGACATCAGCTGGACCAATATCTCCGGCACCATCCCGGACTTCCTCAGCTCCCTCTCCAAGCTCACATACATTCGCCTCTCCTTCAGCAATCTCTCCGGAGCCATCCCGAGCTCCCTCTCCAAGCTCCCAAATCTTGAGTTTCTTCAATTGGACCGTAGCAAACTGACAGGACTAATCCCATATTCTTTCGGGGACTTTAAGGCTAAAGGTTTCTACCTTTATCTTTCCCACAACAAACTTTCCGGCAAAATCCCACCTTCCCTCTCTAGAGTGGACTTCGAATCCGTCGATTTGTCAAGAAACAAGCTTGAAGGCGATGCCTCCATGATATTCGGCGCACACAGGAACACATGGAAAGTTGATTTGTCAAGGAATTTGTTGGAGTTTGACATGTCAAAGGTTGTGTTTTCAAGGAGCTTGTTTATGTTGGATCTTAACCATAATAAGATCTTTGGGGGTTTGCCGCCGCAGATGACGAAGCTCAGTAATCTTAACTCGTTCAATGTGAGTTACAATAGGCTTTGTGGTCAGATTCCAAAGGGTGGAGAGTTGCAGAGATTTGATGTTTATTCGTATTTTCATAATAAGTGTTTGTGTGGTGCACCACTTGAGAGCTGCAAGTGA

Protein sequence

MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK
Homology
BLAST of CmaCh07G013100 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.2e-113
Identity = 202/320 (63.12%), Postives = 243/320 (75.94%), Query Frame = 0

Query: 13  LFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRI 72
           L F      + ++LCNP+DKK LL IKKAF + Y  ASWK + DCC WY V CD  ++RI
Sbjct: 13  LLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTCDSTTNRI 72

Query: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDISWTNI 132
            SL I    ++SGQIP  VGDLP+L++L  HK PNL GPIQPAIAKL  LK L +SWTN+
Sbjct: 73  NSLTIFA-GQVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLRLSWTNL 132

Query: 133 SGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSFGDFK 192
           SG++PDFLS L  LT++ LSF+NL+GAIPSSLS+LPNL  L+LDR+KLTG IP SFG F 
Sbjct: 133 SGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIPISFGQFI 192

Query: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLSRNLL 252
                LYLSHN+LSG IP S +++DF S+DLSRNKLEGDAS+IFG ++ T  VDLSRNLL
Sbjct: 193 GNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVDLSRNLL 252

Query: 253 EFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312
           EF++SKV F  SL  LD+NHNKI+G +P + T+L N    NVSYNRLCGQIP GG+LQ F
Sbjct: 253 EFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQL-NFQFLNVSYNRLCGQIPVGGKLQSF 312

Query: 313 DVYSYFHNKCLCGAPLESCK 333
           D YSYFHN+CLCGAPL SCK
Sbjct: 313 DEYSYFHNRCLCGAPLPSCK 330

BLAST of CmaCh07G013100 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.4e-106
Identity = 190/326 (58.28%), Postives = 240/326 (73.62%), Query Frame = 0

Query: 10  LPLLFFFIFFTISFA-ELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDEN 69
           L LLF F F T   + +LCN NDK  LL IKK+ NN Y+ ASW P+ DCC+WY +EC + 
Sbjct: 7   LCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDA 66

Query: 70  --SHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLD 129
             +HR+ +L I +  +ISGQIP  VGDLP+L++L+  KL NL G IQP IAKL NL+ L 
Sbjct: 67  TVNHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLR 126

Query: 130 ISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPY 189
           +SWTN++G IPDF+S L  L ++ LSF++LSG+IPSSLS LP +  L+L R+KLTG IP 
Sbjct: 127 LSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPE 186

Query: 190 SFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVD 249
           SFG F      L LSHN+LSG IP SL  +DF  +DLSRNKL+GDASM+FG+++ TW +D
Sbjct: 187 SFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSID 246

Query: 250 LSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKG 309
           LSRN+ +FD+SKV   ++L +LDLNHN I G +P Q T+ + L  FNVSYN+LCG IP G
Sbjct: 247 LSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTE-APLQFFNVSYNKLCGHIPTG 306

Query: 310 GELQRFDVYSYFHNKCLCGAPLESCK 333
           G+LQ FD YSYFHNKCLCGAPLE CK
Sbjct: 307 GKLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of CmaCh07G013100 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 379.8 bits (974), Expect = 3.2e-104
Identity = 190/324 (58.64%), Postives = 237/324 (73.15%), Query Frame = 0

Query: 12  LLFFFIFFTISFA-ELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDEN-- 71
           LL   +  T S A +LC+ +DK  LL IKK+ NN Y+ ASW P+ DCC+WY +EC +   
Sbjct: 9   LLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDATV 68

Query: 72  SHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDIS 131
           +HR+ SL I  D EISGQIPP VGDLP+L SL+  KL NL G IQP IAKL NL  L +S
Sbjct: 69  NHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLRLS 128

Query: 132 WTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSF 191
           WTN++G +P+FLS L  L YI LSF++LSG+IPSSLS L  LE+L+L R+KLTG IP SF
Sbjct: 129 WTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPESF 188

Query: 192 GDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLS 251
           G F  K   L+LSHN+LSG IP SL   DF  +DLSRNKL+GDAS++FGA + TW VD+S
Sbjct: 189 GTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVDIS 248

Query: 252 RNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGE 311
           RN+ +FD+SKV  +++L  LD+NHN I G +P + +K +     NVSYNRLCG+IPKG  
Sbjct: 249 RNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSK-AYFQLLNVSYNRLCGRIPKGEY 308

Query: 312 LQRFDVYSYFHNKCLCGAPLESCK 333
           +QRFD YS+FHNKCLCGAPL SCK
Sbjct: 309 IQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of CmaCh07G013100 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.0e-102
Identity = 195/332 (58.73%), Postives = 230/332 (69.28%), Query Frame = 0

Query: 1   MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60
           +LVL    P P          S +E CNP DKK LL IKKA +N Y  ASW P  DCC W
Sbjct: 14  LLVLLATRPCP----------SLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGW 73

Query: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH 120
           Y VECD  +HRI SL I +  ++SGQIP  VGDLPFL++L+  KL NL G I PAIAKL 
Sbjct: 74  YCVECDLTTHRINSLTIFS-GQLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLK 133

Query: 121 NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL 180
           +LK + +SWTN+SG +P F S L  LTY+ LSF+NLSG IP SLS LPNL  L LDR+ L
Sbjct: 134 HLKMVRLSWTNLSGPVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHL 193

Query: 181 TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR 240
           TG IP SFG F      L+LSHN+LSGKIP S    D   +DLSRNKLEGD S+ F A++
Sbjct: 194 TGPIPDSFGKFAGSTPGLHLSHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANK 253

Query: 241 NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC 300
           +T  VD SRNL +FD+S+V F +SL  LDL+HNKI G LP  MT L +L   NVSYNRLC
Sbjct: 254 STQIVDFSRNLFQFDLSRVEFPKSLTSLDLSHNKIAGSLPEMMTSL-DLQFLNVSYNRLC 313

Query: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 333
           G+IP GG+LQ FD  SYFHN+CLCGAPL+SCK
Sbjct: 314 GKIPVGGKLQSFDYDSYFHNRCLCGAPLQSCK 333

BLAST of CmaCh07G013100 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.1e-84
Identity = 163/323 (50.46%), Postives = 212/323 (65.63%), Query Frame = 0

Query: 10  LPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCT-WYSVECDEN 69
           L + F  +F T+  +  C  NDK ALL IKKA  N    +SW P  DCCT W  VEC   
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 70  SHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDIS 129
           + R+  L + T  E+SGQI   +GDL  L++L    LP+L G I   I KL NL  L + 
Sbjct: 67  NRRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLK 126

Query: 130 WTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSF 189
            T++SG IPD++S L  LT++ LSF+  +G IP SLS++P LE +Q++ +KLTG IP SF
Sbjct: 127 HTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSF 186

Query: 190 GDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLS 249
           G F      LYLS+NKLSGKIP SLS+ DF +VDLS N  EGDA M FG ++ T +VDLS
Sbjct: 187 GSFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLS 246

Query: 250 RNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGE 309
           RN+  FD+ KV F+RS+  LDL+ N I+G +PP +TKL +L  FNVS N LCG+IP GG 
Sbjct: 247 RNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKL-HLEHFNVSDNHLCGKIPSGGL 306

Query: 310 LQRFDVYSYFHNKCLCGAPLESC 332
           LQ F+  ++ HN CLCG PL++C
Sbjct: 307 LQTFEPSAFAHNICLCGTPLKAC 325

BLAST of CmaCh07G013100 vs. ExPASy TrEMBL
Match: A0A6J1HMU3 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466050 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.5e-192
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60
           MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW
Sbjct: 1   MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60

Query: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH 120
           YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH
Sbjct: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH 120

Query: 121 NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL 180
           NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL
Sbjct: 121 NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL 180

Query: 181 TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR 240
           TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR
Sbjct: 181 TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR 240

Query: 241 NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC 300
           NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC
Sbjct: 241 NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC 300

Query: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 333
           GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK
Sbjct: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

BLAST of CmaCh07G013100 vs. ExPASy TrEMBL
Match: A0A6J1EB58 (polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432422 PE=4 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 2.8e-188
Identity = 323/332 (97.29%), Postives = 329/332 (99.10%), Query Frame = 0

Query: 1   MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60
           MLVLTLKSPLPLLFFF+FFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW
Sbjct: 1   MLVLTLKSPLPLLFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60

Query: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH 120
           YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPA+AKLH
Sbjct: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPALAKLH 120

Query: 121 NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL 180
           NLKHLDISWTNISGTIPDFLSSL+KLTYIRLSFSNLSGAIPSSLSKLPNL+FLQLDRSKL
Sbjct: 121 NLKHLDISWTNISGTIPDFLSSLTKLTYIRLSFSNLSGAIPSSLSKLPNLKFLQLDRSKL 180

Query: 181 TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR 240
           TG IPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFG HR
Sbjct: 181 TGQIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGVHR 240

Query: 241 NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC 300
           +TWKVDLSRNLLEFDMSKVVFS SLFMLDLNHNKI+GGLPPQMTKLSNLNSFNVSYNRLC
Sbjct: 241 HTWKVDLSRNLLEFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVSYNRLC 300

Query: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 333
           GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK
Sbjct: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

BLAST of CmaCh07G013100 vs. ExPASy TrEMBL
Match: A0A6J1HP52 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466048 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.8e-158
Identity = 280/336 (83.33%), Postives = 300/336 (89.29%), Query Frame = 0

Query: 1   MLVLTLKSPL----PLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEED 60
           M+ L+LKSPL     LLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWK EED
Sbjct: 1   MVRLSLKSPLLSTFSLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKAEED 60

Query: 61  CCTWYSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAI 120
           CCTWYSV+CDENSHRIISL ++ D +ISGQIPP+VG+LPFLQ L  HKLPNLVGPIQPAI
Sbjct: 61  CCTWYSVQCDENSHRIISLTLAVDKQISGQIPPHVGELPFLQYLTFHKLPNLVGPIQPAI 120

Query: 121 AKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLD 180
           AKLHNLKHLDISWT ISGTIPDFLSSLS L+YI LSFSNLSGAIPSSLSKLPNL +LQLD
Sbjct: 121 AKLHNLKHLDISWTKISGTIPDFLSSLSNLSYISLSFSNLSGAIPSSLSKLPNLSYLQLD 180

Query: 181 RSKLTGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIF 240
           RSKLTG IP SFGDFKA+ F LYLSHNKLSGKIPPSLS+VDF ++DLSRNKLEGDA MIF
Sbjct: 181 RSKLTGQIPDSFGDFKAENFNLYLSHNKLSGKIPPSLSKVDFSAIDLSRNKLEGDAYMIF 240

Query: 241 GAHRNTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSY 300
           GA +  W VDLSRNLL FDMSKVVFS +LFMLD+NHNKIFGGLPPQM+KLS L S NVSY
Sbjct: 241 GAQKKAWNVDLSRNLLAFDMSKVVFSTNLFMLDVNHNKIFGGLPPQMSKLS-LTSLNVSY 300

Query: 301 NRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 333
           NRLCGQIPKG +L+RF V SYFHNKCLCG PLESCK
Sbjct: 301 NRLCGQIPKGKKLKRFGVDSYFHNKCLCGKPLESCK 335

BLAST of CmaCh07G013100 vs. ExPASy TrEMBL
Match: A0A6J1HW96 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467229 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 6.2e-127
Identity = 225/321 (70.09%), Postives = 257/321 (80.06%), Query Frame = 0

Query: 12  LLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHR 71
           LL FF+FFTISFAELC+PNDKKALLNIKKAFNN Y   SWKPEEDCCTWY VECD NSHR
Sbjct: 9   LLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRNSHR 68

Query: 72  IISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDISWTN 131
           I+SL I  D  +SGQIPP+VGDLP+L++LM HKLPNL GPIQPAIAKLHNLK+LD+SW  
Sbjct: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNT 128

Query: 132 ISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSFGDF 191
           ++G+IP+FLSSL+ L  + LSF+ L+G+IPSSL+ L  L  L LDR+KLTG IP SFG+F
Sbjct: 129 LTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188

Query: 192 KAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLSRNL 251
           K K  YLYLSHN+LSGKIP S  RVDF  +DLSRN+LEGD S+IFG  + T  VDLSRNL
Sbjct: 189 KGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248

Query: 252 LEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQR 311
           LEFDMSKVV  RSL  LDLNHNKIFG +P     L  L   NVSYN+LCG+IP GG+LQ 
Sbjct: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNL-QLQLLNVSYNKLCGRIPVGGKLQS 308

Query: 312 FDVYSYFHNKCLCGAPLESCK 333
           FDVYSY +NKCLCG PL  CK
Sbjct: 309 FDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmaCh07G013100 vs. ExPASy TrEMBL
Match: A0A6J1ELI8 (polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111435518 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.4e-126
Identity = 227/326 (69.63%), Postives = 260/326 (79.75%), Query Frame = 0

Query: 8   SPLPLLF-FFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECD 67
           SP   LF FF+FFTISFAELC+PNDKKALLNIKKAFNN Y   SWKPEEDCCTWY VECD
Sbjct: 4   SPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECD 63

Query: 68  ENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLD 127
             SHRI+SL I  D  +SGQIPP+VGDLP+L++LM HKLPNL GPIQPAIAKLHNLK+LD
Sbjct: 64  RKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLD 123

Query: 128 ISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPY 187
           +SW +++G+IP+FLSSL+ L  + LSF+ L+G+IPSSL+ L  L  L LDR+KLTG IP 
Sbjct: 124 LSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPD 183

Query: 188 SFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVD 247
           SFG+FK K  YLYLSHN+LSGKIP SL RVDF  +DLSRN+LEGD S+IFG  + T  VD
Sbjct: 184 SFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVD 243

Query: 248 LSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKG 307
           LSRNLLEFDMSKVV  RSL  LDLNHNKIFG +P     L  L   NVSYN+LCG+IP G
Sbjct: 244 LSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNL-QLQLLNVSYNKLCGRIPVG 303

Query: 308 GELQRFDVYSYFHNKCLCGAPLESCK 333
           G+LQ FDVYSY +NKCLCG PL  CK
Sbjct: 304 GKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmaCh07G013100 vs. NCBI nr
Match: XP_022966387.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 681.0 bits (1756), Expect = 5.1e-192
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60
           MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW
Sbjct: 1   MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60

Query: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH 120
           YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH
Sbjct: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH 120

Query: 121 NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL 180
           NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL
Sbjct: 121 NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL 180

Query: 181 TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR 240
           TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR
Sbjct: 181 TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR 240

Query: 241 NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC 300
           NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC
Sbjct: 241 NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC 300

Query: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 333
           GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK
Sbjct: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

BLAST of CmaCh07G013100 vs. NCBI nr
Match: KAG6595655.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 669.8 bits (1727), Expect = 1.2e-188
Identity = 325/332 (97.89%), Postives = 329/332 (99.10%), Query Frame = 0

Query: 1   MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60
           MLVLTLKSPLPLLFFF+FFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW
Sbjct: 1   MLVLTLKSPLPLLFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60

Query: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH 120
           YSV CDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPA+AKLH
Sbjct: 61  YSVHCDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPALAKLH 120

Query: 121 NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL 180
           NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL
Sbjct: 121 NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL 180

Query: 181 TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR 240
           TG IPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR
Sbjct: 181 TGQIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR 240

Query: 241 NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC 300
           +TWKVDLSRNLLEFDMSKVVFS SLFMLDLNHNKI+GGLPPQMTKLSNLNSFNVSYNRLC
Sbjct: 241 HTWKVDLSRNLLEFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVSYNRLC 300

Query: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 333
           GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK
Sbjct: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

BLAST of CmaCh07G013100 vs. NCBI nr
Match: XP_022925061.1 (polygalacturonase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 667.5 bits (1721), Expect = 5.8e-188
Identity = 323/332 (97.29%), Postives = 329/332 (99.10%), Query Frame = 0

Query: 1   MLVLTLKSPLPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60
           MLVLTLKSPLPLLFFF+FFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW
Sbjct: 1   MLVLTLKSPLPLLFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTW 60

Query: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLH 120
           YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPA+AKLH
Sbjct: 61  YSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPALAKLH 120

Query: 121 NLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKL 180
           NLKHLDISWTNISGTIPDFLSSL+KLTYIRLSFSNLSGAIPSSLSKLPNL+FLQLDRSKL
Sbjct: 121 NLKHLDISWTNISGTIPDFLSSLTKLTYIRLSFSNLSGAIPSSLSKLPNLKFLQLDRSKL 180

Query: 181 TGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHR 240
           TG IPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFG HR
Sbjct: 181 TGQIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGVHR 240

Query: 241 NTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLC 300
           +TWKVDLSRNLLEFDMSKVVFS SLFMLDLNHNKI+GGLPPQMTKLSNLNSFNVSYNRLC
Sbjct: 241 HTWKVDLSRNLLEFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVSYNRLC 300

Query: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 333
           GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK
Sbjct: 301 GQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

BLAST of CmaCh07G013100 vs. NCBI nr
Match: XP_023517556.1 (polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 317/336 (94.35%), Postives = 325/336 (96.73%), Query Frame = 0

Query: 1   MLVLTLKSPL----PLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEED 60
           M+ L+LKSPL     LLFFF+FFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEED
Sbjct: 1   MVRLSLKSPLSSTISLLFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEED 60

Query: 61  CCTWYSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAI 120
           CCTWYSV+CDENSHRIISLDISTDNEISGQIPPY+GDLPFLQSLMLHKLPNLVGPIQP I
Sbjct: 61  CCTWYSVDCDENSHRIISLDISTDNEISGQIPPYIGDLPFLQSLMLHKLPNLVGPIQPTI 120

Query: 121 AKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLD 180
           AKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLP+LEFLQLD
Sbjct: 121 AKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPSLEFLQLD 180

Query: 181 RSKLTGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIF 240
           RSKLTG IPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIF
Sbjct: 181 RSKLTGQIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIF 240

Query: 241 GAHRNTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSY 300
           G HR+TWKVDLSRNLLEFDMSKVVFS SLFMLDLNHNKI+GGLPPQMTKL NLNSFNVSY
Sbjct: 241 GEHRHTWKVDLSRNLLEFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLGNLNSFNVSY 300

Query: 301 NRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 333
           NRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK
Sbjct: 301 NRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 336

BLAST of CmaCh07G013100 vs. NCBI nr
Match: XP_022966386.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 566.6 bits (1459), Expect = 1.4e-157
Identity = 280/336 (83.33%), Postives = 300/336 (89.29%), Query Frame = 0

Query: 1   MLVLTLKSPL----PLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEED 60
           M+ L+LKSPL     LLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWK EED
Sbjct: 1   MVRLSLKSPLLSTFSLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKAEED 60

Query: 61  CCTWYSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAI 120
           CCTWYSV+CDENSHRIISL ++ D +ISGQIPP+VG+LPFLQ L  HKLPNLVGPIQPAI
Sbjct: 61  CCTWYSVQCDENSHRIISLTLAVDKQISGQIPPHVGELPFLQYLTFHKLPNLVGPIQPAI 120

Query: 121 AKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLD 180
           AKLHNLKHLDISWT ISGTIPDFLSSLS L+YI LSFSNLSGAIPSSLSKLPNL +LQLD
Sbjct: 121 AKLHNLKHLDISWTKISGTIPDFLSSLSNLSYISLSFSNLSGAIPSSLSKLPNLSYLQLD 180

Query: 181 RSKLTGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIF 240
           RSKLTG IP SFGDFKA+ F LYLSHNKLSGKIPPSLS+VDF ++DLSRNKLEGDA MIF
Sbjct: 181 RSKLTGQIPDSFGDFKAENFNLYLSHNKLSGKIPPSLSKVDFSAIDLSRNKLEGDAYMIF 240

Query: 241 GAHRNTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSY 300
           GA +  W VDLSRNLL FDMSKVVFS +LFMLD+NHNKIFGGLPPQM+KLS L S NVSY
Sbjct: 241 GAQKKAWNVDLSRNLLAFDMSKVVFSTNLFMLDVNHNKIFGGLPPQMSKLS-LTSLNVSY 300

Query: 301 NRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 333
           NRLCGQIPKG +L+RF V SYFHNKCLCG PLESCK
Sbjct: 301 NRLCGQIPKGKKLKRFGVDSYFHNKCLCGKPLESCK 335

BLAST of CmaCh07G013100 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 386.3 bits (991), Expect = 2.4e-107
Identity = 190/326 (58.28%), Postives = 240/326 (73.62%), Query Frame = 0

Query: 10  LPLLFFFIFFTISFA-ELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDEN 69
           L LLF F F T   + +LCN NDK  LL IKK+ NN Y+ ASW P+ DCC+WY +EC + 
Sbjct: 7   LCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDA 66

Query: 70  --SHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLD 129
             +HR+ +L I +  +ISGQIP  VGDLP+L++L+  KL NL G IQP IAKL NL+ L 
Sbjct: 67  TVNHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLR 126

Query: 130 ISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPY 189
           +SWTN++G IPDF+S L  L ++ LSF++LSG+IPSSLS LP +  L+L R+KLTG IP 
Sbjct: 127 LSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPE 186

Query: 190 SFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVD 249
           SFG F      L LSHN+LSG IP SL  +DF  +DLSRNKL+GDASM+FG+++ TW +D
Sbjct: 187 SFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSID 246

Query: 250 LSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKG 309
           LSRN+ +FD+SKV   ++L +LDLNHN I G +P Q T+ + L  FNVSYN+LCG IP G
Sbjct: 247 LSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTE-APLQFFNVSYNKLCGHIPTG 306

Query: 310 GELQRFDVYSYFHNKCLCGAPLESCK 333
           G+LQ FD YSYFHNKCLCGAPLE CK
Sbjct: 307 GKLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of CmaCh07G013100 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 379.8 bits (974), Expect = 2.3e-105
Identity = 190/324 (58.64%), Postives = 237/324 (73.15%), Query Frame = 0

Query: 12  LLFFFIFFTISFA-ELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDEN-- 71
           LL   +  T S A +LC+ +DK  LL IKK+ NN Y+ ASW P+ DCC+WY +EC +   
Sbjct: 9   LLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDATV 68

Query: 72  SHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDIS 131
           +HR+ SL I  D EISGQIPP VGDLP+L SL+  KL NL G IQP IAKL NL  L +S
Sbjct: 69  NHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLRLS 128

Query: 132 WTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSF 191
           WTN++G +P+FLS L  L YI LSF++LSG+IPSSLS L  LE+L+L R+KLTG IP SF
Sbjct: 129 WTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPESF 188

Query: 192 GDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLS 251
           G F  K   L+LSHN+LSG IP SL   DF  +DLSRNKL+GDAS++FGA + TW VD+S
Sbjct: 189 GTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVDIS 248

Query: 252 RNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGE 311
           RN+ +FD+SKV  +++L  LD+NHN I G +P + +K +     NVSYNRLCG+IPKG  
Sbjct: 249 RNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSK-AYFQLLNVSYNRLCGRIPKGEY 308

Query: 312 LQRFDVYSYFHNKCLCGAPLESCK 333
           +QRFD YS+FHNKCLCGAPL SCK
Sbjct: 309 IQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of CmaCh07G013100 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 312.0 bits (798), Expect = 5.8e-85
Identity = 163/323 (50.46%), Postives = 212/323 (65.63%), Query Frame = 0

Query: 10  LPLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCT-WYSVECDEN 69
           L + F  +F T+  +  C  NDK ALL IKKA  N    +SW P  DCCT W  VEC   
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 70  SHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDIS 129
           + R+  L + T  E+SGQI   +GDL  L++L    LP+L G I   I KL NL  L + 
Sbjct: 67  NRRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLK 126

Query: 130 WTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSF 189
            T++SG IPD++S L  LT++ LSF+  +G IP SLS++P LE +Q++ +KLTG IP SF
Sbjct: 127 HTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSF 186

Query: 190 GDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLS 249
           G F      LYLS+NKLSGKIP SLS+ DF +VDLS N  EGDA M FG ++ T +VDLS
Sbjct: 187 GSFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLS 246

Query: 250 RNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGE 309
           RN+  FD+ KV F+RS+  LDL+ N I+G +PP +TKL +L  FNVS N LCG+IP GG 
Sbjct: 247 RNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKL-HLEHFNVSDNHLCGKIPSGGL 306

Query: 310 LQRFDVYSYFHNKCLCGAPLESC 332
           LQ F+  ++ HN CLCG PL++C
Sbjct: 307 LQTFEPSAFAHNICLCGTPLKAC 325

BLAST of CmaCh07G013100 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 186.0 bits (471), Expect = 4.8e-47
Identity = 120/365 (32.88%), Postives = 184/365 (50.41%), Query Frame = 0

Query: 6   LKSPLPLLFFFIFFT-ISFAELCNPNDKKALLNIKKAFN--NAYYFASWKPEEDCC-TWY 65
           L SP   L   +F + IS    C+P D+ AL   K + +  N   F +W    DCC  WY
Sbjct: 5   LASPFSSLLAVVFISVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEWY 64

Query: 66  SVECDENSHRIISLDISTDNE------------ISGQIPPYVGDLPFLQSLMLHKLPNLV 125
            + CD +S R+  + +  ++E            +SG I P V DL  L SL+L     + 
Sbjct: 65  GISCDPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGIT 124

Query: 126 GPIQPAIAKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPN 185
           G I P I  L +L+ LD++   I+G IP  +  LSKL  + L+ + +SG IP+SL+ L  
Sbjct: 125 GEIPPCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLIE 184

Query: 186 LEFLQLDRSKLTGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVD-FESVDLSRNKL 245
           L+ L+L  + +TG+IP  FG  K     L L  N+L+G IP S+S ++    +DLS+N +
Sbjct: 185 LKHLELTENGITGVIPADFGSLKMLSRVL-LGRNELTGSIPESISGMERLADLDLSKNHI 244

Query: 246 EG-----------------DASMIFG-------AHRNTWKVDLSRNLLEFDMSKVVFSRS 305
           EG                 D + + G       ++      +LSRN LE  +  V  S++
Sbjct: 245 EGPIPEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSKT 304

Query: 306 -LFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRFDVYSYFHNKCL 329
            L  LDL+HN + G +P  ++    +   ++S+N+LCG+IP G      +  S+  N+CL
Sbjct: 305 YLVSLDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDNQCL 364

BLAST of CmaCh07G013100 vs. TAIR 10
Match: AT5G12940.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.2e-42
Identity = 115/369 (31.17%), Postives = 180/369 (48.78%), Query Frame = 0

Query: 5   TLKSPLPLLFFFIFFTI--SFAELCNPNDKKALLNIKKAFNNAY--YFASWKPEEDCCTW 64
           T KS + LL   + F +  +    C P+D+ ALL  +   N  Y   F +WK  + C  W
Sbjct: 3   TKKSLVILLTNVVVFLLLSTTVHSCLPSDRAALLEFRAKLNEPYIGVFNTWKGLDCCKGW 62

Query: 65  YSVECDENSHRIISLDISTDNE------------ISGQIPPYVGDLPFLQSLMLHKLPNL 124
           Y V CD N+ R+  + +  ++E            ++G I P +  L  L  +++     +
Sbjct: 63  YGVSCDPNTRRVAGITLRGESEDPLFQKAKRSGLMTGSISPSICKLTRLSGIIIADWKGI 122

Query: 125 VGPIQPAIAKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLP 184
            G I   I  L  L+HLD+     SG IP  +  L +L  + L+ ++L G IP S+++L 
Sbjct: 123 SGVIPSCIENLPFLRHLDLVGNKFSGVIPANIGKLLRLKVLNLADNHLYGVIPPSITRLV 182

Query: 185 NLEFLQLDRSKLTGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRV-DFESVDLSRNK 244
           +L  L L  + ++G+IP   G  K     L LS NK+SG+IP SL+R+     ++LS N+
Sbjct: 183 SLSHLDLRNNNISGVIPRDIGRLKMVSRVL-LSGNKISGQIPDSLTRIYRLADLELSMNR 242

Query: 245 LEG-----------------DASMIFG------AHRNTWKVDLSRNLLEFDMSKVVFSRS 304
           L G                 D ++I G         +   ++LS NL+   +      RS
Sbjct: 243 LTGPIPASFGKMSVLATLNLDGNLISGMIPGSLLASSISNLNLSGNLITGSIPNTFGPRS 302

Query: 305 LF-MLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRFDVYSYFHNKCL 333
            F +LDL +N++ G +P  +T  S +   +VS+N LCG+IP G      D  S+ +N CL
Sbjct: 303 YFTVLDLANNRLQGPIPASITAASFIGHLDVSHNHLCGKIPMGSPFDHLDATSFAYNACL 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q050912.2e-11363.13Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1[more]
Q9M5J93.4e-10658.28Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1[more]
Q9M5J83.2e-10458.64Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2[more]
A7PW811.0e-10258.73Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1[more]
Q9LH528.1e-8450.46Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HMU32.5e-192100.00polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466050 PE=... [more]
A0A6J1EB582.8e-18897.29polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432422 P... [more]
A0A6J1HP526.8e-15883.33polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466048 PE=... [more]
A0A6J1HW966.2e-12770.09polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467229 PE=... [more]
A0A6J1ELI81.4e-12669.63polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111435518 P... [more]
Match NameE-valueIdentityDescription
XP_022966387.15.1e-192100.00polygalacturonase inhibitor-like [Cucurbita maxima][more]
KAG6595655.11.2e-18897.89Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022925061.15.8e-18897.29polygalacturonase inhibitor-like [Cucurbita moschata][more]
XP_023517556.11.3e-18294.35polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo][more]
XP_022966386.11.4e-15783.33polygalacturonase inhibitor-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G06860.12.4e-10758.28polygalacturonase inhibiting protein 1 [more]
AT5G06870.12.3e-10558.64polygalacturonase inhibiting protein 2 [more]
AT3G12145.15.8e-8550.46Leucine-rich repeat (LRR) family protein [more]
AT3G12610.14.8e-4732.88Leucine-rich repeat (LRR) family protein [more]
AT5G12940.11.2e-4231.17Leucine-rich repeat (LRR) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 121..180
e-value: 3.2E-7
score: 30.0
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 197..214
e-value: 1.6
score: 9.6
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 29..66
e-value: 4.9E-10
score: 39.6
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 25..332
e-value: 8.1E-84
score: 283.4
NoneNo IPR availablePANTHERPTHR48059:SF10POLYGALACTURONASE INHIBITOR 2coord: 10..331
NoneNo IPR availablePANTHERPTHR48059POLYGALACTURONASE INHIBITOR 1coord: 10..331
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 26..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G013100.1CmaCh07G013100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0090353 polygalacturonase inhibitor activity
molecular_function GO:0005515 protein binding