CmaCh07G011760 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G011760
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCma_Chr07: 6454855 .. 6456390 (-)
RNA-Seq ExpressionCmaCh07G011760
SyntenyCmaCh07G011760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGAAGTTGATCCTCCTCCCTTCTTCCTTTGTCCCATCTCTTTGCAAATTATGAAAGATCCCGTCACGGTCGCCTCCGGCATAACCTACGATCGGGAGAACATCGAGAGATGGTTGTTTTTCGATAAACATACGACGTGTCCGGTCACGCAAGTGGTCTTGTCGGACTTTGATATCACTCCCAATAATACTCTCTCCCGTTTGATTCAAGCTTGGAGCACTCTCAACGATGGGGTCGACAGGATTCCGACACCGAAGCCTCCGATTGACAAGAAACAAGTCATCAAGATTCTTGCAAATGTGAAGCTGTCTCCTTTCTCTCAACAGAATTGTTTGCAACGACTCCGGTCTATCGCATCAACAAACGAGACGAATAAACGTTGTATGGAAGCTGCTGGAGCGGTGGAGTTCTTGGTTGGAGTTGCGTGTAATTCCATTACCATAGAATCGAACTTTCAAGGTACTTCTAAATTACAAATTTTAAGTATGGACGTTTTTTAGATCCAACTCAATTTCTTTTAACCCAAATAAAAATGAACTCAACCCAACCATAAATTTTTTTGGGATAGTTGGGGTCAATTATTCAAAAAATTTTTAATAAAAATAATATAAATTTATAATTTGGCGAGAATTTATTAAACTATTTACTTAATTGACCACTAAATTAAAATATTGTTGACACAGATTTCGCGTTTGACGATCCGTTAGCGAATTTGGGCGATGAGGCATTAAGCATTCTTTACAAACTTCAAATCTCTGAATCCGGCCTCAAGTTTCTTCTCGCCAACAATGGCGGCATCTTCGTCACAACCCTAACAAAATTCTTACAAAACGGCACTTATGGCTCCCGAGCGTATGCAACAATGCTTCTTCAATCGATGTTCGAAGTCGCCGATGAGATTCAGATTCGCAGTCTAAATCTTGAATTCTTCGTTGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCCTCCAAATCGGCATTGAAATTGCTAATCGGACTCTGTTCGAGTTTCAGAAACAGAGTAAATGCAGTTCAAGCTGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTGGATCTCGATTTGTCGGAGAAGCGTTTGTGCGAGATGATTCTCGTGGCGCTTGATCTGCTCTGCAGCTGTGCTGACGGCAGAGCGGAGCTATTGAACCACGCGGCGGGGATCGCCGTCGTGTCGAAGAAGATTCTCAGAGTTTCGTCGCTTGGAAGCGAGAAGGCCGTCGGAATTTTGTGCTCTATTGCGAGATTCTCGGCGAGCCAATCCGTCGTTCAGGAAATGGTGCGGCTCGGAGTTGTGATGAAGCTCTGTTTTCTGCTTCAAGTCGTCGGCGCCGGAGCGAAAGCGAAAGAGAAGGCGAAAGAGATTCTAAAGCTTCATGGTCGAACATGGCGGAATTCTTCATGTTTTCCTTCGAGTTTGCGACCTTCTTATCCATTTTAGGGTTTTTTTTTTCTTTTTTTTTTTCGCAAAACTTCATTTCTTTCAAAGTGCATATAGTTAGAGC

mRNA sequence

ATGGCAGAAGTTGATCCTCCTCCCTTCTTCCTTTGTCCCATCTCTTTGCAAATTATGAAAGATCCCGTCACGGTCGCCTCCGGCATAACCTACGATCGGGAGAACATCGAGAGATGGTTGTTTTTCGATAAACATACGACGTGTCCGGTCACGCAAGTGGTCTTGTCGGACTTTGATATCACTCCCAATAATACTCTCTCCCGTTTGATTCAAGCTTGGAGCACTCTCAACGATGGGGTCGACAGGATTCCGACACCGAAGCCTCCGATTGACAAGAAACAAGTCATCAAGATTCTTGCAAATGTGAAGCTGTCTCCTTTCTCTCAACAGAATTGTTTGCAACGACTCCGGTCTATCGCATCAACAAACGAGACGAATAAACGTTGTATGGAAGCTGCTGGAGCGGTGGAGTTCTTGGTTGGAGTTGCGTATTTCGCGTTTGACGATCCGTTAGCGAATTTGGGCGATGAGGCATTAAGCATTCTTTACAAACTTCAAATCTCTGAATCCGGCCTCAAGTTTCTTCTCGCCAACAATGGCGGCATCTTCGTCACAACCCTAACAAAATTCTTACAAAACGGCACTTATGGCTCCCGAGCGTATGCAACAATGCTTCTTCAATCGATGTTCGAAGTCGCCGATGAGATTCAGATTCGCAGTCTAAATCTTGAATTCTTCGTTGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCCTCCAAATCGGCATTGAAATTGCTAATCGGACTCTGTTCGAGTTTCAGAAACAGAGTAAATGCAGTTCAAGCTGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTGGATCTCGATTTGTCGGAGAAGCGTTTGTGCGAGATGATTCTCGTGGCGCTTGATCTGCTCTGCAGCTGTGCTGACGGCAGAGCGGAGCTATTGAACCACGCGGCGGGGATCGCCGTCGTGTCGAAGAAGATTCTCAGAGTTTCGTCGCTTGGAAGCGAGAAGGCCGTCGGAATTTTGTGCTCTATTGCGAGATTCTCGGCGAGCCAATCCGTCGTTCAGGAAATGGTGCGGCTCGGAGTTGTGATGAAGCTCTGTTTTCTGCTTCAAGTCGTCGGCGCCGGAGCGAAAGCGAAAGAGAAGGCGAAAGAGATTCTAAAGCTTCATGGTCGAACATGGCGGAATTCTTCATGTTTTCCTTCGAGTTTGCGACCTTCTTATCCATTTTAGGGTTTTTTTTTTCTTTTTTTTTTTCGCAAAACTTCATTTCTTTCAAAGTGCATATAGTTAGAGC

Coding sequence (CDS)

ATGGCAGAAGTTGATCCTCCTCCCTTCTTCCTTTGTCCCATCTCTTTGCAAATTATGAAAGATCCCGTCACGGTCGCCTCCGGCATAACCTACGATCGGGAGAACATCGAGAGATGGTTGTTTTTCGATAAACATACGACGTGTCCGGTCACGCAAGTGGTCTTGTCGGACTTTGATATCACTCCCAATAATACTCTCTCCCGTTTGATTCAAGCTTGGAGCACTCTCAACGATGGGGTCGACAGGATTCCGACACCGAAGCCTCCGATTGACAAGAAACAAGTCATCAAGATTCTTGCAAATGTGAAGCTGTCTCCTTTCTCTCAACAGAATTGTTTGCAACGACTCCGGTCTATCGCATCAACAAACGAGACGAATAAACGTTGTATGGAAGCTGCTGGAGCGGTGGAGTTCTTGGTTGGAGTTGCGTATTTCGCGTTTGACGATCCGTTAGCGAATTTGGGCGATGAGGCATTAAGCATTCTTTACAAACTTCAAATCTCTGAATCCGGCCTCAAGTTTCTTCTCGCCAACAATGGCGGCATCTTCGTCACAACCCTAACAAAATTCTTACAAAACGGCACTTATGGCTCCCGAGCGTATGCAACAATGCTTCTTCAATCGATGTTCGAAGTCGCCGATGAGATTCAGATTCGCAGTCTAAATCTTGAATTCTTCGTTGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCCTCCAAATCGGCATTGAAATTGCTAATCGGACTCTGTTCGAGTTTCAGAAACAGAGTAAATGCAGTTCAAGCTGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTGGATCTCGATTTGTCGGAGAAGCGTTTGTGCGAGATGATTCTCGTGGCGCTTGATCTGCTCTGCAGCTGTGCTGACGGCAGAGCGGAGCTATTGAACCACGCGGCGGGGATCGCCGTCGTGTCGAAGAAGATTCTCAGAGTTTCGTCGCTTGGAAGCGAGAAGGCCGTCGGAATTTTGTGCTCTATTGCGAGATTCTCGGCGAGCCAATCCGTCGTTCAGGAAATGGTGCGGCTCGGAGTTGTGATGAAGCTCTGTTTTCTGCTTCAAGTCGTCGGCGCCGGAGCGAAAGCGAAAGAGAAGGCGAAAGAGATTCTAAAGCTTCATGGTCGAACATGGCGGAATTCTTCATGTTTTCCTTCGAGTTTGCGACCTTCTTATCCATTTTAG

Protein sequence

MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIASTNETNKRCMEAAGAVEFLVGVAYFAFDDPLANLGDEALSILYKLQISESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMILVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF
Homology
BLAST of CmaCh07G011760 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.5e-111
Identity = 217/405 (53.58%), Postives = 292/405 (72.10%), Query Frame = 0

Query: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62
           E++ PPFFLCPISL+IMKDPV V++GITYDR++IE+WLF  K  +CPVT+  ++D D+TP
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 63  NNTLSRLIQAWSTLND--GVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 122
           N+TL RLIQ+W TLN   GV+RIPTP+PPI K ++ K++ +   S  +Q  CL+RLR I 
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIV 128

Query: 123 STNETNKRCMEAAGAVEFLVGVAYFAFDDPLANLGDEALSILYKLQISESGLKFLLAN-N 182
           S N TNKRC+EAAG  EFL  +   + D    +L DEAL++LY L+ SE+ LK LL N  
Sbjct: 129 SENATNKRCLEAAGVPEFLANIV--SNDSENGSLTDEALNLLYHLETSETVLKNLLNNKK 188

Query: 183 GGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEIVQILKDQISK 242
               V +LTK +Q G Y SR YAT+LL+++ EVAD +Q  +L  E F E+VQIL D+IS+
Sbjct: 189 DNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRISQ 248

Query: 243 QASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDL-SEKRLCEMILVALDLLCS 302
           +A+K+A+ +L+ +C   RNR  AV+AG + V+I+LL+D    SE+R  EM +V LDLLC 
Sbjct: 249 KATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLLCQ 308

Query: 303 CADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVRLGVVM 362
           CA+GRAE LNH A IAVV KKILRVS   S++AV +L S+ RF A+ +++ EM++LGVV 
Sbjct: 309 CAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGVVA 368

Query: 363 KLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 404
           KLC +LQ V  G K KEKAKE+LKLH R W++S C P ++  +YP
Sbjct: 369 KLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of CmaCh07G011760 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 3.3e-111
Identity = 225/432 (52.08%), Postives = 289/432 (66.90%), Query Frame = 0

Query: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62
           E++ P FFLCPISL IMKDPV V++GITYDRE+IE+WLF  K  +CPVT+ V+++ D+TP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 63  NNTLSRLIQAWSTLND--GVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 122
           N+TL RLIQ+W TLN   G++RIPTPKPPI K ++ K++     S  +Q  CL+RLR I 
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIV 123

Query: 123 STNETNKRCMEAAGAVEFLVGVAYFAFD---------------------------DPLAN 182
           S N TNKRC+EAA   EFL  +   + D                           D   +
Sbjct: 124 SENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRS 183

Query: 183 LGDEALSILYKLQISESGLKFLLANNGGI-FVTTLTKFLQNGTYGSRAYATMLLQSMFEV 242
           L DEALS+LY L  SE+ LK LL N  G   V TLTK +Q G Y SRAYA +LL+ + EV
Sbjct: 184 LMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEV 243

Query: 243 ADEIQIRSLNLEFFVEIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLI 302
           AD +QI  L  E F E++QIL DQIS +A++SA+++L+  C   RNR  AV+ G + ++I
Sbjct: 244 ADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMII 303

Query: 303 DLLLDLDL-SEKRLCEMILVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKA 362
           +LL+D    SE+R  EM +V LD+LC CA+GRAE LNH A IAVVSKKILRVS + SE+A
Sbjct: 304 ELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSERA 363

Query: 363 VGILCSIARFSASQSVVQEMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNS 404
           V +L S+ RF A+ S++QEM++LGVV KLC +LQ V  G K KEKAKE+LKLH R WR S
Sbjct: 364 VRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWRES 423

BLAST of CmaCh07G011760 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 4.2e-58
Identity = 161/442 (36.43%), Postives = 233/442 (52.71%), Query Frame = 0

Query: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLS-DFDIT 62
           E++ P +F+CPISL+IMKDPVT  SGITYDR+NI +WL  +K  +CPVT+  L  D D+T
Sbjct: 7   EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLT 66

Query: 63  PNNTLSRLIQAWSTLND--GVDRIPTPKPPIDKKQVIKILANVK---LSPFSQQNCLQRL 122
           PN+ L RLIQ W   N+  GV RI TP+ P  K  V++ + N+K        ++  LQ+L
Sbjct: 67  PNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKL 126

Query: 123 RSIASTNETNKRCMEAAGAVEFLVGVAYFAFDDP-----LANLGDEALSILYKLQISESG 182
             +A      +   E       ++ V     +D      +  L DE+L +L+ + I  + 
Sbjct: 127 EVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGL-DESLRLLHLIGIPSND 186

Query: 183 LKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEIVQ 242
            K +L  N  + + +LT  L    + S+AY  +LL+++ E      +  LN E F  I+ 
Sbjct: 187 AKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGIIG 246

Query: 243 ILKDQIS-----------------------------------KQASKSALKLLIGLCSSF 302
            LKD ++                                   KQA  +AL +L+   S  
Sbjct: 247 FLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSWS 306

Query: 303 RNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMILVALDLLCSCADGRAELLNHAAGIAVV 362
           RNR   V  GAV  LI+L +     EKR+ E++L  L  LC CA+GRAE+L H  GIAVV
Sbjct: 307 RNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVV 366

Query: 363 SKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVRLGVVMKLCFLLQVVGAGAKAKEK 399
           +K++LRVS    ++A+ IL ++++FS    VV+EMV +G V KLC +L  +  G   KEK
Sbjct: 367 TKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLG-MDCGLNLKEK 426

BLAST of CmaCh07G011760 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.8e-46
Identity = 140/404 (34.65%), Postives = 219/404 (54.21%), Query Frame = 0

Query: 7   PPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITPNNTL 66
           P  F CPISL++M+DPVTV +G TYDR +IE W+    +TTCPVT+  LSDF + PN+TL
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHTL 74

Query: 67  SRLIQAWSTLN--DGVDRIPTPKPPIDKKQVIKILANVK------LSPFSQQNCLQRLRS 126
            RLIQ W   N  +GV+RIPTPK P D   V  +L+         +S  S+   L+RLR 
Sbjct: 75  RRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLRG 134

Query: 127 IASTNETNKRCMEAAGAVEFLVGVAYFAFDDPLANLGDEALSILYKLQISESGLKFLLAN 186
            A  ++ N+  + A  A E L+ + +   +   + L  E+L++L  L I+E      +++
Sbjct: 135 FARDSDKNRVLIAAHNATEILIKILF--SETTSSELVSESLALLVMLPITEPNQFVSISS 194

Query: 187 NGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIR---SLNLEFFVEIVQILKD 246
           + G  V  LT+ L + +  +R  A  L++ +        ++   S +   F  ++ +L++
Sbjct: 195 DPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDLLRN 254

Query: 247 QI-SKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLID-LLLDLDLSEKRLCEMILVAL 306
            I S++A K  +K L  LCS    R  A+ AGA  +LID L  D D  +    E  L  +
Sbjct: 255 PISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD---TERALATV 314

Query: 307 DLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVR 366
           +LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A +   +E   
Sbjct: 315 ELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALCTAEERWREEAAG 374

Query: 367 LGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSS 398
            GVV++L  ++Q      +AK+KA+++LKL   +W + + F +S
Sbjct: 375 AGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNSFANS 409

BLAST of CmaCh07G011760 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 9.4e-42
Identity = 135/401 (33.67%), Postives = 213/401 (53.12%), Query Frame = 0

Query: 7   PPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITPNNTL 66
           P  F CPISL +M DPVT+++G TYDR +I+ W+    +TTCPVT+V LSDF + PN+TL
Sbjct: 15  PYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMG-NTTCPVTRVALSDFTLIPNHTL 74

Query: 67  SRLIQAWSTLN--DGVDRIPTPKPPIDKKQVIKILANVK------LSPFSQQNCLQRLRS 126
            RLIQ W   N  +GV+RIPTPK P D   V  +L+         +S  S+   ++RLR 
Sbjct: 75  RRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRLRG 134

Query: 127 IASTNETNKRCMEAAGAVEFLVGVAYFAFDDP--LANLGDEALSILYKLQISESGLKFLL 186
           +A  +E N+  +    A E LV + +   +     + L  E+L++L  L ++E+  + + 
Sbjct: 135 LARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLHMTETECEAVA 194

Query: 187 ANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIR---SLNLEFFVEIVQIL 246
           ++     V  +T+ L + +   R  A  L++ +   A  + ++   S +   F  ++ +L
Sbjct: 195 SDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVLDLL 254

Query: 247 KDQI-SKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLID-LLLDLDLSEKRLCEMILV 306
           K+ I S++A K  +K +  LC   + R  A+ AGA  +LID L  D D  +    E  L 
Sbjct: 255 KNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD---TERGLA 314

Query: 307 ALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEM 366
            ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A +    E 
Sbjct: 315 TVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALCTAEERCRDEA 374

Query: 367 VRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSS 393
              G+V +L  L+Q      +AK KA+ +LKL   +W + S
Sbjct: 375 AAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 406

BLAST of CmaCh07G011760 vs. ExPASy TrEMBL
Match: A0A6J1HP11 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466021 PE=4 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 9.2e-218
Identity = 403/415 (97.11%), Postives = 403/415 (97.11%), Query Frame = 0

Query: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI 60
           MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI
Sbjct: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI 60

Query: 61  TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 120
           TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA
Sbjct: 61  TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 120

Query: 121 STNETNKRCMEAAGAVEFLVGVAY-----------FAFDDPLANLGDEALSILYKLQISE 180
           STNETNKRCMEAAGAVEFLVGVA            FAFDDPLANLGDEALSILYKLQISE
Sbjct: 121 STNETNKRCMEAAGAVEFLVGVACNSITIESNFQDFAFDDPLANLGDEALSILYKLQISE 180

Query: 181 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 240
           SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI
Sbjct: 181 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 240

Query: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI 300
           VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI
Sbjct: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI 300

Query: 301 LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360
           LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ
Sbjct: 301 LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360

Query: 361 EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF 405
           EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF
Sbjct: 361 EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF 415

BLAST of CmaCh07G011760 vs. ExPASy TrEMBL
Match: A0A6J1EEF1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478 PE=4 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.0e-208
Identity = 385/415 (92.77%), Postives = 393/415 (94.70%), Query Frame = 0

Query: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI 60
           MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLF DKHTTCPVTQVVLSDFDI
Sbjct: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDI 60

Query: 61  TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 120
           TPNNTLSRLIQAW T+N+ V+RIPTPKPP+DKKQV+KILA+VKLSPFSQQNCL+RL SIA
Sbjct: 61  TPNNTLSRLIQAWCTVNNRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIA 120

Query: 121 STNETNKRCMEAAGAVEFLVGVAY-----------FAFDDPLANLGDEALSILYKLQISE 180
           S NE NKRCMEAAGAVEFLVGV             FAFDDPLANLGDEALSILYKLQISE
Sbjct: 121 SANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAFDDPLANLGDEALSILYKLQISE 180

Query: 181 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 240
           SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI
Sbjct: 181 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 240

Query: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI 300
           VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLD SEKRLCEMI
Sbjct: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEMI 300

Query: 301 LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360
           LVALDLLC CADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ
Sbjct: 301 LVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360

Query: 361 EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF 405
           EM RLGVV+KLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF
Sbjct: 361 EMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF 415

BLAST of CmaCh07G011760 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 6.7e-184
Identity = 341/416 (81.97%), Postives = 370/416 (88.94%), Query Frame = 0

Query: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62
           E+DPPPFFLCPISLQIMKDPVTVASGITYDRE+IERWLF D+H TCPVTQVVLS FDITP
Sbjct: 7   ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFDITP 66

Query: 63  NNTLSRLIQAWSTLN--DGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 122
           N+T+ R+IQAW T+N   G++RIPTPKPPID KQV++ILA+ KLSPFSQQNCL+RLRSIA
Sbjct: 67  NHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLRRLRSIA 126

Query: 123 STNETNKRCMEAAGAVEFLVGVA-------------YFAFDDPLANLGDEALSILYKLQI 182
           + +E+NKRCMEAAGAVEFL G+               +  DD  ANLGDEALSILYKLQI
Sbjct: 127 AASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSILYKLQI 186

Query: 183 SESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFV 242
           SESGLKFLLANNGGIFV+TLTK LQNGTYGSRAY+ MLL SMFEVAD+IQI SLN +FF+
Sbjct: 187 SESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFFI 246

Query: 243 EIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCE 302
           EIVQILKDQISKQASKSALKLLIGLCSS RNRV AVQAGA+PVLIDLLLDLD SEKR CE
Sbjct: 247 EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSSEKRSCE 306

Query: 303 MILVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSV 362
           MILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS+LGSEKAVGILCSIARFSAS+SV
Sbjct: 307 MILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESV 366

Query: 363 VQEMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 404
           VQEM+RLGVV KLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSC PSSLR S+P
Sbjct: 367 VQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFP 422

BLAST of CmaCh07G011760 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 6.9e-181
Identity = 337/416 (81.01%), Postives = 368/416 (88.46%), Query Frame = 0

Query: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62
           E+DPPPFFLCPISLQIMKDPVTVASGITYDRE+IERWLF D+H TCP+TQVVLS+FDITP
Sbjct: 7   ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLSNFDITP 66

Query: 63  NNTLSRLIQAWSTLN--DGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 122
           N+TL R+IQAW T+N   GV+RIPTPKPP+D KQV++ILA+ KLS FSQQNCL+RL+SIA
Sbjct: 67  NHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLRRLQSIA 126

Query: 123 STNETNKRCMEAAGAVEFLVGVA-------------YFAFDDPLANLGDEALSILYKLQI 182
           + +E+NKRCMEAAGAVEFL G+               +  DD LANLG EALSILYKLQI
Sbjct: 127 AASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSILYKLQI 186

Query: 183 SESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFV 242
           SESGLKFLLANNGGIFV+TLTK LQNGTYGSR Y+ MLL SMFEVAD+IQI SLN +FFV
Sbjct: 187 SESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSLNTDFFV 246

Query: 243 EIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCE 302
           EIVQILKDQISKQASKSALKLLIGLCSS RNRV AV+AGAVPVLIDLLLDLD SEKR CE
Sbjct: 247 EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCE 306

Query: 303 MILVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSV 362
           MILV LDLLC CADGRAE+LNHAAGIA+VSKKILRVS+LGSEKAVGILCSIARFSAS+SV
Sbjct: 307 MILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESV 366

Query: 363 VQEMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 404
           VQEM+RLGVV KLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSC PSSLR S+P
Sbjct: 367 VQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFP 422

BLAST of CmaCh07G011760 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 1.0e-171
Identity = 319/415 (76.87%), Postives = 361/415 (86.99%), Query Frame = 0

Query: 4   VDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITPN 63
           VDPPP+FLCPISLQIMKDPVT+ASGITYDRE+IE+WLF DKH TCPV+ +VLSDFD+TPN
Sbjct: 11  VDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDFDVTPN 70

Query: 64  NTLSRLIQAWSTLN--DGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIAS 123
           +TL R+IQAW T+N   GV+RIPTPKPP+D++QV++ILA+ KLSPFSQ+NCL RLRSIA+
Sbjct: 71  HTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRLRSIAA 130

Query: 124 TNETNKRCMEAAGAVEFLVGVAY------------FAFDDPLANLGDEALSILYKLQISE 183
            NE+NKRCME+AG VEFL GV              +AFDD  AN  DEAL IL+KLQISE
Sbjct: 131 ANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHKLQISE 190

Query: 184 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 243
           S LKFLL+NNGGIF++TLTK LQN +Y SR+Y+ MLL SMFEVAD+IQI++L  +FF+EI
Sbjct: 191 SSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFFIEI 250

Query: 244 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSE-KRLCEM 303
           +QILKDQISKQASKSALKLLI + SSFRNRV AVQAGAVPVLIDLLLDLD SE KRLCEM
Sbjct: 251 IQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEM 310

Query: 304 ILVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVV 363
           ILV LD LC CADGRAELLNHAAGIAVVSKKILR+S++GSEKAVGIL SIARFS SQSVV
Sbjct: 311 ILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSGSQSVV 370

Query: 364 QEMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 404
           QEMVR+GVV KLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSC PS+LR +YP
Sbjct: 371 QEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 425

BLAST of CmaCh07G011760 vs. NCBI nr
Match: XP_022966336.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 765.8 bits (1976), Expect = 1.9e-217
Identity = 403/415 (97.11%), Postives = 403/415 (97.11%), Query Frame = 0

Query: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI 60
           MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI
Sbjct: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI 60

Query: 61  TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 120
           TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA
Sbjct: 61  TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 120

Query: 121 STNETNKRCMEAAGAVEFLVGVAY-----------FAFDDPLANLGDEALSILYKLQISE 180
           STNETNKRCMEAAGAVEFLVGVA            FAFDDPLANLGDEALSILYKLQISE
Sbjct: 121 STNETNKRCMEAAGAVEFLVGVACNSITIESNFQDFAFDDPLANLGDEALSILYKLQISE 180

Query: 181 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 240
           SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI
Sbjct: 181 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 240

Query: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI 300
           VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI
Sbjct: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI 300

Query: 301 LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360
           LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ
Sbjct: 301 LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360

Query: 361 EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF 405
           EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF
Sbjct: 361 EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF 415

BLAST of CmaCh07G011760 vs. NCBI nr
Match: XP_022925148.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 734.2 bits (1894), Expect = 6.2e-208
Identity = 385/415 (92.77%), Postives = 393/415 (94.70%), Query Frame = 0

Query: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI 60
           MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLF DKHTTCPVTQVVLSDFDI
Sbjct: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDI 60

Query: 61  TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 120
           TPNNTLSRLIQAW T+N+ V+RIPTPKPP+DKKQV+KILA+VKLSPFSQQNCL+RL SIA
Sbjct: 61  TPNNTLSRLIQAWCTVNNRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIA 120

Query: 121 STNETNKRCMEAAGAVEFLVGVAY-----------FAFDDPLANLGDEALSILYKLQISE 180
           S NE NKRCMEAAGAVEFLVGV             FAFDDPLANLGDEALSILYKLQISE
Sbjct: 121 SANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAFDDPLANLGDEALSILYKLQISE 180

Query: 181 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 240
           SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI
Sbjct: 181 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 240

Query: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI 300
           VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLD SEKRLCEMI
Sbjct: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEMI 300

Query: 301 LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360
           LVALDLLC CADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ
Sbjct: 301 LVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360

Query: 361 EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF 405
           EM RLGVV+KLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF
Sbjct: 361 EMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYPF 415

BLAST of CmaCh07G011760 vs. NCBI nr
Match: XP_023518739.1 (E3 ubiquitin-protein ligase PUB22-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 716.8 bits (1849), Expect = 1.0e-202
Identity = 375/412 (91.02%), Postives = 388/412 (94.17%), Query Frame = 0

Query: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI 60
           MAE+DPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLF DKHTTCPVTQVVLSDFDI
Sbjct: 1   MAEIDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDI 60

Query: 61  TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 120
           TPNNTLSRLIQAW T+N+ V+RIPTPKPP+DKKQ++KILA+VKLSPFSQQNCL+RL SIA
Sbjct: 61  TPNNTLSRLIQAWCTVNNRVERIPTPKPPVDKKQIVKILADVKLSPFSQQNCLRRLGSIA 120

Query: 121 STNETNKRCMEAAGAVEFLVGVAY-----------FAFDDPLANLGDEALSILYKLQISE 180
           S NE NKRCMEAAGAVEFLVGV             F FDDPLANLGD+ALSILYKLQISE
Sbjct: 121 SANEMNKRCMEAAGAVEFLVGVVCNSITIESSFQDFTFDDPLANLGDKALSILYKLQISE 180

Query: 181 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 240
           SGLKFLLANNGGIFV TLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIR+LNLEFFVEI
Sbjct: 181 SGLKFLLANNGGIFVKTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRTLNLEFFVEI 240

Query: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI 300
           VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLD SEKRLCE+I
Sbjct: 241 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEII 300

Query: 301 LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360
           LVALDLLC CADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ
Sbjct: 301 LVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 360

Query: 361 EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPS 402
           EM RLGVV+KLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPS
Sbjct: 361 EMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPS 412

BLAST of CmaCh07G011760 vs. NCBI nr
Match: KAG6595515.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 654.8 bits (1688), Expect = 4.7e-184
Identity = 348/396 (87.88%), Postives = 357/396 (90.15%), Query Frame = 0

Query: 1   MAEVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI 60
           MAEVDPPPFFLCPISL IMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI
Sbjct: 1   MAEVDPPPFFLCPISLGIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDI 60

Query: 61  TPNNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 120
           TPNNTLSRLIQAW T+N+ V+RIPTPKPP+DKK+V+KILANVKLS FSQQN         
Sbjct: 61  TPNNTLSRLIQAWCTVNNRVERIPTPKPPVDKKEVVKILANVKLSLFSQQN--------- 120

Query: 121 STNETNKRCMEAAGAVEFLVGVAYFAFDDPLANLGDEALSILYKLQISESGLKFLLANNG 180
                                  YFAFDDPLANLGDEALSILYKLQISESGLKFLLANNG
Sbjct: 121 -----------------------YFAFDDPLANLGDEALSILYKLQISESGLKFLLANNG 180

Query: 181 GIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEIVQILKDQISKQ 240
           GIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVAD IQIR+LNL+FFVEIVQILKDQISKQ
Sbjct: 181 GIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADAIQIRTLNLQFFVEIVQILKDQISKQ 240

Query: 241 ASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMILVALDLLCSCA 300
           ASKSALKLLIGLCSSFRNRV+AVQAGAVPVLIDLLLDLD SEKRLCEMILVALDLLC CA
Sbjct: 241 ASKSALKLLIGLCSSFRNRVHAVQAGAVPVLIDLLLDLDSSEKRLCEMILVALDLLCGCA 300

Query: 301 DGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVRLGVVMKL 360
           DGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVRLGVVMKL
Sbjct: 301 DGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVRLGVVMKL 360

Query: 361 CFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPS 397
           CFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPS
Sbjct: 361 CFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPS 364

BLAST of CmaCh07G011760 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 653.3 bits (1684), Expect = 1.4e-183
Identity = 341/416 (81.97%), Postives = 370/416 (88.94%), Query Frame = 0

Query: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62
           E+DPPPFFLCPISLQIMKDPVTVASGITYDRE+IERWLF D+H TCPVTQVVLS FDITP
Sbjct: 7   ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLSSFDITP 66

Query: 63  NNTLSRLIQAWSTLN--DGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 122
           N+T+ R+IQAW T+N   G++RIPTPKPPID KQV++ILA+ KLSPFSQQNCL+RLRSIA
Sbjct: 67  NHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLRRLRSIA 126

Query: 123 STNETNKRCMEAAGAVEFLVGVA-------------YFAFDDPLANLGDEALSILYKLQI 182
           + +E+NKRCMEAAGAVEFL G+               +  DD  ANLGDEALSILYKLQI
Sbjct: 127 AASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSILYKLQI 186

Query: 183 SESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFV 242
           SESGLKFLLANNGGIFV+TLTK LQNGTYGSRAY+ MLL SMFEVAD+IQI SLN +FF+
Sbjct: 187 SESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFFI 246

Query: 243 EIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCE 302
           EIVQILKDQISKQASKSALKLLIGLCSS RNRV AVQAGA+PVLIDLLLDLD SEKR CE
Sbjct: 247 EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSSEKRSCE 306

Query: 303 MILVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSV 362
           MILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS+LGSEKAVGILCSIARFSAS+SV
Sbjct: 307 MILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESV 366

Query: 363 VQEMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 404
           VQEM+RLGVV KLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSC PSSLR S+P
Sbjct: 367 VQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFP 422

BLAST of CmaCh07G011760 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 404.4 bits (1038), Expect = 1.0e-112
Identity = 217/405 (53.58%), Postives = 292/405 (72.10%), Query Frame = 0

Query: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62
           E++ PPFFLCPISL+IMKDPV V++GITYDR++IE+WLF  K  +CPVT+  ++D D+TP
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 63  NNTLSRLIQAWSTLND--GVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 122
           N+TL RLIQ+W TLN   GV+RIPTP+PPI K ++ K++ +   S  +Q  CL+RLR I 
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIV 128

Query: 123 STNETNKRCMEAAGAVEFLVGVAYFAFDDPLANLGDEALSILYKLQISESGLKFLLAN-N 182
           S N TNKRC+EAAG  EFL  +   + D    +L DEAL++LY L+ SE+ LK LL N  
Sbjct: 129 SENATNKRCLEAAGVPEFLANIV--SNDSENGSLTDEALNLLYHLETSETVLKNLLNNKK 188

Query: 183 GGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEIVQILKDQISK 242
               V +LTK +Q G Y SR YAT+LL+++ EVAD +Q  +L  E F E+VQIL D+IS+
Sbjct: 189 DNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRISQ 248

Query: 243 QASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDL-SEKRLCEMILVALDLLCS 302
           +A+K+A+ +L+ +C   RNR  AV+AG + V+I+LL+D    SE+R  EM +V LDLLC 
Sbjct: 249 KATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLLCQ 308

Query: 303 CADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVRLGVVM 362
           CA+GRAE LNH A IAVV KKILRVS   S++AV +L S+ RF A+ +++ EM++LGVV 
Sbjct: 309 CAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGVVA 368

Query: 363 KLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 404
           KLC +LQ V  G K KEKAKE+LKLH R W++S C P ++  +YP
Sbjct: 369 KLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of CmaCh07G011760 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 403.3 bits (1035), Expect = 2.3e-112
Identity = 225/432 (52.08%), Postives = 289/432 (66.90%), Query Frame = 0

Query: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62
           E++ P FFLCPISL IMKDPV V++GITYDRE+IE+WLF  K  +CPVT+ V+++ D+TP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 63  NNTLSRLIQAWSTLND--GVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 122
           N+TL RLIQ+W TLN   G++RIPTPKPPI K ++ K++     S  +Q  CL+RLR I 
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIV 123

Query: 123 STNETNKRCMEAAGAVEFLVGVAYFAFD---------------------------DPLAN 182
           S N TNKRC+EAA   EFL  +   + D                           D   +
Sbjct: 124 SENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRS 183

Query: 183 LGDEALSILYKLQISESGLKFLLANNGGI-FVTTLTKFLQNGTYGSRAYATMLLQSMFEV 242
           L DEALS+LY L  SE+ LK LL N  G   V TLTK +Q G Y SRAYA +LL+ + EV
Sbjct: 184 LMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEV 243

Query: 243 ADEIQIRSLNLEFFVEIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLI 302
           AD +QI  L  E F E++QIL DQIS +A++SA+++L+  C   RNR  AV+ G + ++I
Sbjct: 244 ADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMII 303

Query: 303 DLLLDLDL-SEKRLCEMILVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKA 362
           +LL+D    SE+R  EM +V LD+LC CA+GRAE LNH A IAVVSKKILRVS + SE+A
Sbjct: 304 ELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSERA 363

Query: 363 VGILCSIARFSASQSVVQEMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNS 404
           V +L S+ RF A+ S++QEM++LGVV KLC +LQ V  G K KEKAKE+LKLH R WR S
Sbjct: 364 VRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWRES 423

BLAST of CmaCh07G011760 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 226.9 bits (577), Expect = 3.0e-59
Identity = 161/442 (36.43%), Postives = 233/442 (52.71%), Query Frame = 0

Query: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLS-DFDIT 62
           E++ P +F+CPISL+IMKDPVT  SGITYDR+NI +WL  +K  +CPVT+  L  D D+T
Sbjct: 21  EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLT 80

Query: 63  PNNTLSRLIQAWSTLND--GVDRIPTPKPPIDKKQVIKILANVK---LSPFSQQNCLQRL 122
           PN+ L RLIQ W   N+  GV RI TP+ P  K  V++ + N+K        ++  LQ+L
Sbjct: 81  PNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKL 140

Query: 123 RSIASTNETNKRCMEAAGAVEFLVGVAYFAFDDP-----LANLGDEALSILYKLQISESG 182
             +A      +   E       ++ V     +D      +  L DE+L +L+ + I  + 
Sbjct: 141 EVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGL-DESLRLLHLIGIPSND 200

Query: 183 LKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEIVQ 242
            K +L  N  + + +LT  L    + S+AY  +LL+++ E      +  LN E F  I+ 
Sbjct: 201 AKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGIIG 260

Query: 243 ILKDQIS-----------------------------------KQASKSALKLLIGLCSSF 302
            LKD ++                                   KQA  +AL +L+   S  
Sbjct: 261 FLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSWS 320

Query: 303 RNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMILVALDLLCSCADGRAELLNHAAGIAVV 362
           RNR   V  GAV  LI+L +     EKR+ E++L  L  LC CA+GRAE+L H  GIAVV
Sbjct: 321 RNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVV 380

Query: 363 SKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVRLGVVMKLCFLLQVVGAGAKAKEK 399
           +K++LRVS    ++A+ IL ++++FS    VV+EMV +G V KLC +L  +  G   KEK
Sbjct: 381 TKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLG-MDCGLNLKEK 440

BLAST of CmaCh07G011760 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 186.8 bits (473), Expect = 3.4e-47
Identity = 140/404 (34.65%), Postives = 219/404 (54.21%), Query Frame = 0

Query: 7   PPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITPNNTL 66
           P  F CPISL++M+DPVTV +G TYDR +IE W+    +TTCPVT+  LSDF + PN+TL
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHTL 74

Query: 67  SRLIQAWSTLN--DGVDRIPTPKPPIDKKQVIKILANVK------LSPFSQQNCLQRLRS 126
            RLIQ W   N  +GV+RIPTPK P D   V  +L+         +S  S+   L+RLR 
Sbjct: 75  RRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLRG 134

Query: 127 IASTNETNKRCMEAAGAVEFLVGVAYFAFDDPLANLGDEALSILYKLQISESGLKFLLAN 186
            A  ++ N+  + A  A E L+ + +   +   + L  E+L++L  L I+E      +++
Sbjct: 135 FARDSDKNRVLIAAHNATEILIKILF--SETTSSELVSESLALLVMLPITEPNQFVSISS 194

Query: 187 NGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIR---SLNLEFFVEIVQILKD 246
           + G  V  LT+ L + +  +R  A  L++ +        ++   S +   F  ++ +L++
Sbjct: 195 DPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDLLRN 254

Query: 247 QI-SKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLID-LLLDLDLSEKRLCEMILVAL 306
            I S++A K  +K L  LCS    R  A+ AGA  +LID L  D D  +    E  L  +
Sbjct: 255 PISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD---TERALATV 314

Query: 307 DLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEMVR 366
           +LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A +   +E   
Sbjct: 315 ELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALCTAEERWREEAAG 374

Query: 367 LGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSS 398
            GVV++L  ++Q      +AK+KA+++LKL   +W + + F +S
Sbjct: 375 AGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNSFANS 409

BLAST of CmaCh07G011760 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 172.6 bits (436), Expect = 6.7e-43
Identity = 135/401 (33.67%), Postives = 213/401 (53.12%), Query Frame = 0

Query: 7   PPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITPNNTL 66
           P  F CPISL +M DPVT+++G TYDR +I+ W+    +TTCPVT+V LSDF + PN+TL
Sbjct: 15  PYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMG-NTTCPVTRVALSDFTLIPNHTL 74

Query: 67  SRLIQAWSTLN--DGVDRIPTPKPPIDKKQVIKILANVK------LSPFSQQNCLQRLRS 126
            RLIQ W   N  +GV+RIPTPK P D   V  +L+         +S  S+   ++RLR 
Sbjct: 75  RRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRLRG 134

Query: 127 IASTNETNKRCMEAAGAVEFLVGVAYFAFDDP--LANLGDEALSILYKLQISESGLKFLL 186
           +A  +E N+  +    A E LV + +   +     + L  E+L++L  L ++E+  + + 
Sbjct: 135 LARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLHMTETECEAVA 194

Query: 187 ANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIR---SLNLEFFVEIVQIL 246
           ++     V  +T+ L + +   R  A  L++ +   A  + ++   S +   F  ++ +L
Sbjct: 195 SDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVLDLL 254

Query: 247 KDQI-SKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLID-LLLDLDLSEKRLCEMILV 306
           K+ I S++A K  +K +  LC   + R  A+ AGA  +LID L  D D  +    E  L 
Sbjct: 255 KNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD---TERGLA 314

Query: 307 ALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQEM 366
            ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A +    E 
Sbjct: 315 TVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALCTAEERCRDEA 374

Query: 367 VRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSS 393
              G+V +L  L+Q      +AK KA+ +LKL   +W + S
Sbjct: 375 AAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 406

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84TG31.5e-11153.58E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC63.3e-11152.08E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF154.2e-5836.43E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT794.8e-4634.65U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA49.4e-4233.67U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1HP119.2e-21897.11RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466021 P... [more]
A0A6J1EEF13.0e-20892.77RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478... [more]
A0A6J1IKG76.7e-18481.97RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 P... [more]
A0A6J1GGQ86.9e-18181.01RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790... [more]
A0A5A7UVZ11.0e-17176.87RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
XP_022966336.11.9e-21797.11E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima][more]
XP_022925148.16.2e-20892.77E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata][more]
XP_023518739.11.0e-20291.02E3 ubiquitin-protein ligase PUB22-like, partial [Cucurbita pepo subsp. pepo][more]
KAG6595515.14.7e-18487.88E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022978192.11.4e-18381.97E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G35930.11.0e-11253.58plant U-box 23 [more]
AT3G52450.12.3e-11252.08plant U-box 22 [more]
AT3G11840.13.0e-5936.43plant U-box 24 [more]
AT3G19380.13.4e-4734.65plant U-box 25 [more]
AT1G49780.16.7e-4333.67plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 9..73
e-value: 2.2E-28
score: 110.3
IPR003613U box domainPFAMPF04564U-boxcoord: 7..74
e-value: 8.1E-18
score: 64.4
IPR003613U box domainPROSITEPS51698U_BOXcoord: 5..80
score: 40.674294
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 88..385
e-value: 3.8E-17
score: 63.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..81
e-value: 4.5E-25
score: 89.5
NoneNo IPR availablePANTHERPTHR22849:SF132E3 UBIQUITIN-PROTEIN LIGASE PUB23coord: 1..404
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 1..404
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 10..52
e-value: 1.08915E-21
score: 85.363
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 3..75
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 105..361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G011760.1CmaCh07G011760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity