CmaCh07G011540 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G011540
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionnucleolar MIF4G domain-containing protein 1-like
LocationCma_Chr07: 6327719 .. 6331237 (-)
RNA-Seq ExpressionCmaCh07G011540
SyntenyCmaCh07G011540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGACGGCGAGAATGGGGAAGGAGGATTTGAAACTCCGGTCTCGAACCAAGGCGAAAACGAAGTTCGAAGAGTATTTAGATATGGAGATGGGCATGCAGAACCATCGATTGTCCGCTGAGGAGGACTTAGAATTAGAAAGAAAATTTGCACAGAAACTAAAGGTCAAGGCTGGAAAGTTAAGGGGAGTAGAAGATGGTATGAATGTGTTATTTGAAGGACTCCCTTCGATTGTTGATTCTCCTGGGGAAGACGAACTTCCCTTTTCTAAAGAGTTTGCAGTTGGGCGAAGTAGGAAGAACAAGGCTTTGAATAAGAAGGAGAAGAAAAGGAATTTGTTAGAACAATCTCCAGAGAGTGAAATTGAGAAAGCAAGCGTGGGCGATGGTGAAGTGGAAGAAACTTTAGAGAAAAGTAAAAAACGGAAGAAAAAGATGTCAGGACAACATCAAGTTACTAATGATGATGGTGTGGAATCCCCTTGTGCTGCACAGGTTGAGGTACAAAAACTTCATGTCAAGCCCCAAAAATATATTGCTCCTAACTTGAGATTGCACAAAGGAACGGAGCCTGAAGATCATACTCAACTCCGCAGGCGTGTTCGAGGTGGGTAGCTTTTATGATTATTGCTCCAATTTTTTTCCCTGCATGTTCTTCAATTTCAAATATAATCTGCAATTTTTCGAATTTTTCAGGTCTTTTAAACAGGCTTTCCGAATCAAATGTGGAATCAGTAACAGGGGAAATGTCTACTGTCTATCACGTATGATATTTTCTTTTAACTTTCTTGTTTGTTCATATCAAATTTTGATTATCATTAGGAAAGGTGTCCGTGGAATGTTACCAAATATGGAATTAATTGTGATGTATCAATTTTTGCAGTCTATTTCACGCAGCATTGCTTCTCAAATTATTAGTGAGGAGGTTCTGGCATCTTGTTCGCGCGGTCCTCGTGGCAATGAACAGTTGAGTGTCTGTTAATATTGTTATCAACCTTCCCTTACCACTTTTTGGCTTGTTAATTTACCCTAACTTGTTTGTTTCTTGTGTAAGGTATGCTGCTGTTTTCGCGGCTTTTGTTGCAGGTATGGGTTGTTTGGTTGGTTCGGACTTTAGCGCAAGACTCATGGCCTCTCTTGCTAACACATTTGAGGTGAGGCTGCCACCACCTGAATACGTCCTTAAATGTTTGATTTAAATTTTGAGAATTATTAAGAAAATCATATATCTTTTTCAGGAAGAATACCGGAAAGAAGATAATCTTTCTTTACGGAATCTTACACTATTGCTTTCCTATTTGTGTGTGTTTGAAGCTTGTGCTAGGTACGTAAATATGCAGATTATTTTGTATATTTCATTATACAATGCATATATCCACATCTTTGTAATACGCATTATTGTATCTTTTGATGCTTTTCAGTGATCTGATCTATGATTTTCTGATTATACTGAGCAAGCGATTGACAGAAATCGACGTGTCCACCATCCTAACAGTTCTACAATGTAAGCTGATATCGAGTTTGTCGATCTCTCACTAGTGTTAATTGTTATATTACTGAAACATTGTTAATTCTAATTTGATAGGCTGTGGAATGAAAATAAGGGGTGATGATCCTTGTGCAATGAAGAATTTCATTGTGAGTGTTCAAAGTAAGGTTAATGAGGTGAAGGCGGCCTCACGAGATGATCAACAAAACATCAATGGAAAGAGAGTGAGTTTGTATATGTAATATTATTTGGTTTACCCTTTGTTTTATTTGTTTGAATCTTTGTAAGATTATAATGTGGATGGATGCAGATGGAGTTCATGCTCGAGACTATATGTGACATCAAGAATAACAAGAAGAAACCCAAGGACGATCCTGCAAACCATACGCGGATAAAGAAATGGCTACACAAGGTCTTTCTCTTCTATTTCATTGACTTGCTTCTATGTAGGTTACCCTATTTTTTAGTGTAGAGCTTAACTTGCTAAGTACTTGCTCGTTCTAGACTCGATATTTTAATATATTAAGTAGCCACCATGTGAGATCCCACGTCGGTTGGAGAGGGGAACGAAACATTCCTTGAGGGGAAGCCCAGAAGGTTAAGTCCAGAGAGGACAATATCTACCACACAGCTTGGGTCTTTCCCTAGAGGACAATATCTACCACAGCTTGGGTCTTTCCCTAGAGGACAATATCTACCACAGCTTGGGCTAGCTTGAGCTTGGGCTGTTACACACCATGAGTTTTGGTATATTCTCCCAAGAAAAGCCCTTTTACTTCCTTTTGCATCTACTAATGTTACTAGGCTAACTCATGGTTTACTCTATTTGGAGTCAGTTGACAAGCCTTAAGATGCGTTAGACATAGAAAGGACATGAGAAGTTGGGTAGTGTATGTATATATATTTAAAGAAAGAGCGAGATTCTACGCTCGAGCTATGTAACCTTGCCTTGTCAGTCATGATATCTATAGAAAAACAGTGTGATTCCTGTCTGACCTTTTGCTCCTTTAATTATTTGTTTTCAGTTAGGAGTAGAAGACATAATAATTAGAGGCATAAAATGGAAAAAGCTGCTCGATCCCGACAAGAAGGGACAGTGGTGGTTAACTGGAGATGTAATGACAACATCAGAAAACATTACAGAGTTTGCCAGCACGATCGACACCGAGGTTCTTGAAGCTCAAAAAATGCTTCAACTTGCAGCTGCACAGAGAATGAATACAGATGCTAGAAAGGCAATATTCTGTATTATAATGAGTGGAGACGATTATGTTGATGCATTTGAAAAGCTTTTAAGATTGGATTTATCTGGAAAGCAGGTTTGTAGAATATATTGAAAATTTTGTTCGTCTCGTCTCATCTCATCTGTTCTATTCTGTTGACACTATCTCTCATAATCCTACAGGACAGGGAGATAATGAGGGTTCTTGTGGACTGCTGCTTACAGGAGAAAGTGTTTAACAAGTACTACACTATTCTAGCTTCCAAACTGTGTGAGCACGAAAAAAACCATAAGTTTACGTTACAGGTAAATTATTCTTTTCGTTTCGTTTTTCTTTGGTCTAAACTTGTTTTGTCGATCGAACTTCGATACGTTTGTGAAATTATTATCACCTTTTTGACTCTGTCACACTTCTTCGCCACAGTATTGCCTATGGGATCACTTCAAAGAATTGGAAACGATGCAGCTTGTTAGGTCGATGAATCTTGCGAGATTCACGGCCGAGATGATTGCTTCGTATACTCTCTCCCTCGCAGTTTTGAAGTCTGTGGATCTGAGTGATATCATGCTTCTTACTCCAAAGAGGATCATGCATTTCCGTATGATGTTTGATTCGATCTTTGAACGTCCTGACAAGTTGATTTGGAACGCGTTCACTCGAGTCGCTATTAACCCTGAGCTCGAGCCTCTTCGAAGCGGTATGTTGTTCTTCATTAAAGAGTATATGATTAAGGGTAATAAAGTTAATGTAGAGAAGTTCAAGGTTGTAAAGAAAGCCCTCAACAATGTTGAGGGAATCCTTATGTAA

mRNA sequence

ATGAGGACGGCGAGAATGGGGAAGGAGGATTTGAAACTCCGGTCTCGAACCAAGGCGAAAACGAAGTTCGAAGAGTATTTAGATATGGAGATGGGCATGCAGAACCATCGATTGTCCGCTGAGGAGGACTTAGAATTAGAAAGAAAATTTGCACAGAAACTAAAGGTCAAGGCTGGAAAGTTAAGGGGAGTAGAAGATGGTATGAATGTGTTATTTGAAGGACTCCCTTCGATTGTTGATTCTCCTGGGGAAGACGAACTTCCCTTTTCTAAAGAGTTTGCAGTTGGGCGAAGTAGGAAGAACAAGGCTTTGAATAAGAAGGAGAAGAAAAGGAATTTGTTAGAACAATCTCCAGAGAGTGAAATTGAGAAAGCAAGCGTGGGCGATGGTGAAGTGGAAGAAACTTTAGAGAAAAGTAAAAAACGGAAGAAAAAGATGTCAGGACAACATCAAGTTACTAATGATGATGGTGTGGAATCCCCTTGTGCTGCACAGGTTGAGGTACAAAAACTTCATGTCAAGCCCCAAAAATATATTGCTCCTAACTTGAGATTGCACAAAGGAACGGAGCCTGAAGATCATACTCAACTCCGCAGGCGTGTTCGAGGTCTTTTAAACAGGCTTTCCGAATCAAATGTGGAATCAGTAACAGGGGAAATGTCTACTGTCTATCACTCTATTTCACGCAGCATTGCTTCTCAAATTATTAGTGAGGAGGTTCTGGCATCTTGTTCGCGCGGTCCTCGTGGCAATGAACAGTATGCTGCTGTTTTCGCGGCTTTTGTTGCAGGTATGGGTTGTTTGGTTGGTTCGGACTTTAGCGCAAGACTCATGGCCTCTCTTGCTAACACATTTGAGGAAGAATACCGGAAAGAAGATAATCTTTCTTTACGGAATCTTACACTATTGCTTTCCTATTTGTGTGTGTTTGAAGCTTGTGCTAGTGATCTGATCTATGATTTTCTGATTATACTGAGCAAGCGATTGACAGAAATCGACGTGTCCACCATCCTAACAGTTCTACAATGCTGTGGAATGAAAATAAGGGGTGATGATCCTTGTGCAATGAAGAATTTCATTGTGAGTGTTCAAAGTAAGGTTAATGAGGTGAAGGCGGCCTCACGAGATGATCAACAAAACATCAATGGAAAGAGAATGGAGTTCATGCTCGAGACTATATGTGACATCAAGAATAACAAGAAGAAACCCAAGGACGATCCTGCAAACCATACGCGGATAAAGAAATGGCTACACAAGTTAGGAGTAGAAGACATAATAATTAGAGGCATAAAATGGAAAAAGCTGCTCGATCCCGACAAGAAGGGACAGTGGTGGTTAACTGGAGATGTAATGACAACATCAGAAAACATTACAGAGTTTGCCAGCACGATCGACACCGAGGTTCTTGAAGCTCAAAAAATGCTTCAACTTGCAGCTGCACAGAGAATGAATACAGATGCTAGAAAGGCAATATTCTGTATTATAATGAGTGGAGACGATTATGTTGATGCATTTGAAAAGCTTTTAAGATTGGATTTATCTGGAAAGCAGGACAGGGAGATAATGAGGGTTCTTGTGGACTGCTGCTTACAGGAGAAAGTGTTTAACAAGTACTACACTATTCTAGCTTCCAAACTGTGTGAGCACGAAAAAAACCATAAGTTTACGTTACAGTATTGCCTATGGGATCACTTCAAAGAATTGGAAACGATGCAGCTTGTTAGGTCGATGAATCTTGCGAGATTCACGGCCGAGATGATTGCTTCGTATACTCTCTCCCTCGCAGTTTTGAAGTCTGTGGATCTGAGTGATATCATGCTTCTTACTCCAAAGAGGATCATGCATTTCCGTATGATGTTTGATTCGATCTTTGAACGTCCTGACAAGTTGATTTGGAACGCGTTCACTCGAGTCGCTATTAACCCTGAGCTCGAGCCTCTTCGAAGCGGTATGTTGTTCTTCATTAAAGAGTATATGATTAAGGGTAATAAAGTTAATGTAGAGAAGTTCAAGGTTGTAAAGAAAGCCCTCAACAATGTTGAGGGAATCCTTATGTAA

Coding sequence (CDS)

ATGAGGACGGCGAGAATGGGGAAGGAGGATTTGAAACTCCGGTCTCGAACCAAGGCGAAAACGAAGTTCGAAGAGTATTTAGATATGGAGATGGGCATGCAGAACCATCGATTGTCCGCTGAGGAGGACTTAGAATTAGAAAGAAAATTTGCACAGAAACTAAAGGTCAAGGCTGGAAAGTTAAGGGGAGTAGAAGATGGTATGAATGTGTTATTTGAAGGACTCCCTTCGATTGTTGATTCTCCTGGGGAAGACGAACTTCCCTTTTCTAAAGAGTTTGCAGTTGGGCGAAGTAGGAAGAACAAGGCTTTGAATAAGAAGGAGAAGAAAAGGAATTTGTTAGAACAATCTCCAGAGAGTGAAATTGAGAAAGCAAGCGTGGGCGATGGTGAAGTGGAAGAAACTTTAGAGAAAAGTAAAAAACGGAAGAAAAAGATGTCAGGACAACATCAAGTTACTAATGATGATGGTGTGGAATCCCCTTGTGCTGCACAGGTTGAGGTACAAAAACTTCATGTCAAGCCCCAAAAATATATTGCTCCTAACTTGAGATTGCACAAAGGAACGGAGCCTGAAGATCATACTCAACTCCGCAGGCGTGTTCGAGGTCTTTTAAACAGGCTTTCCGAATCAAATGTGGAATCAGTAACAGGGGAAATGTCTACTGTCTATCACTCTATTTCACGCAGCATTGCTTCTCAAATTATTAGTGAGGAGGTTCTGGCATCTTGTTCGCGCGGTCCTCGTGGCAATGAACAGTATGCTGCTGTTTTCGCGGCTTTTGTTGCAGGTATGGGTTGTTTGGTTGGTTCGGACTTTAGCGCAAGACTCATGGCCTCTCTTGCTAACACATTTGAGGAAGAATACCGGAAAGAAGATAATCTTTCTTTACGGAATCTTACACTATTGCTTTCCTATTTGTGTGTGTTTGAAGCTTGTGCTAGTGATCTGATCTATGATTTTCTGATTATACTGAGCAAGCGATTGACAGAAATCGACGTGTCCACCATCCTAACAGTTCTACAATGCTGTGGAATGAAAATAAGGGGTGATGATCCTTGTGCAATGAAGAATTTCATTGTGAGTGTTCAAAGTAAGGTTAATGAGGTGAAGGCGGCCTCACGAGATGATCAACAAAACATCAATGGAAAGAGAATGGAGTTCATGCTCGAGACTATATGTGACATCAAGAATAACAAGAAGAAACCCAAGGACGATCCTGCAAACCATACGCGGATAAAGAAATGGCTACACAAGTTAGGAGTAGAAGACATAATAATTAGAGGCATAAAATGGAAAAAGCTGCTCGATCCCGACAAGAAGGGACAGTGGTGGTTAACTGGAGATGTAATGACAACATCAGAAAACATTACAGAGTTTGCCAGCACGATCGACACCGAGGTTCTTGAAGCTCAAAAAATGCTTCAACTTGCAGCTGCACAGAGAATGAATACAGATGCTAGAAAGGCAATATTCTGTATTATAATGAGTGGAGACGATTATGTTGATGCATTTGAAAAGCTTTTAAGATTGGATTTATCTGGAAAGCAGGACAGGGAGATAATGAGGGTTCTTGTGGACTGCTGCTTACAGGAGAAAGTGTTTAACAAGTACTACACTATTCTAGCTTCCAAACTGTGTGAGCACGAAAAAAACCATAAGTTTACGTTACAGTATTGCCTATGGGATCACTTCAAAGAATTGGAAACGATGCAGCTTGTTAGGTCGATGAATCTTGCGAGATTCACGGCCGAGATGATTGCTTCGTATACTCTCTCCCTCGCAGTTTTGAAGTCTGTGGATCTGAGTGATATCATGCTTCTTACTCCAAAGAGGATCATGCATTTCCGTATGATGTTTGATTCGATCTTTGAACGTCCTGACAAGTTGATTTGGAACGCGTTCACTCGAGTCGCTATTAACCCTGAGCTCGAGCCTCTTCGAAGCGGTATGTTGTTCTTCATTAAAGAGTATATGATTAAGGGTAATAAAGTTAATGTAGAGAAGTTCAAGGTTGTAAAGAAAGCCCTCAACAATGTTGAGGGAATCCTTATGTAA

Protein sequence

MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGKLRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPESEIEKASVGDGEVEETLEKSKKRKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIAPNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEVLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRNLTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFIVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSVDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYMIKGNKVNVEKFKVVKKALNNVEGILM
Homology
BLAST of CmaCh07G011540 vs. ExPASy Swiss-Prot
Match: Q5C9Z4 (Nucleolar MIF4G domain-containing protein 1 OS=Homo sapiens OX=9606 GN=NOM1 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.5e-65
Identity = 201/659 (30.50%), Postives = 329/659 (49.92%), Query Frame = 0

Query: 41  EEDLE---LERKFA-QKLKVKAGK----LRGVEDGMNVLFEGLPS-----IVDSPGEDE- 100
           EED E   LER     K K K G     L    DG++ +   L S     + DS GE+E 
Sbjct: 181 EEDREIRKLERCLGLNKRKKKDGSSSVPLSFARDGLDYILGALESGKNSGLYDSSGEEEE 240

Query: 101 -----LPFSKEFAVGRSRKNKALN---KKEKKRNLLEQSPESEIEKASVGDGEVEETLEK 160
                LP S   +  +    +      +KEKK    E   E + E      GE +E   +
Sbjct: 241 DAGQTLPESDLESDSQDESEEEEEGDVEKEKKAQEAEAQSEDDDEDTEEEQGEEKEKGAQ 300

Query: 161 SKKRKKKMS-GQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIAPNLRLHKGT----EPED 220
            K+R K++   + +  +++  E      +  + L    +KYI P++R  + T    + E+
Sbjct: 301 EKRRGKRVRFAEDEEKSENSSED---GDITDKSLCGSGEKYIPPHVRQAEETVDFKKKEE 360

Query: 221 HTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEVLASCSRGPRGNEQ 280
             +L++ V+GLLNRLSE N+ S++G++  +Y + SR   +  ++  ++ +C        +
Sbjct: 361 LERLKKHVKGLLNRLSEPNMASISGQLEELYMAHSRKDMNDTLTSALMGACVTASAMPSR 420

Query: 281 YAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYR-KEDNLSLRNLTLLLSYLCVFEA 340
                   V+ +   VG +  A  + ++   F+  Y+   +     NL  ++++L  F  
Sbjct: 421 LMMEHVLLVSILHHTVGIEVGAHFLEAVVRKFDAIYKYGSEGKECDNLFTVIAHLYNFHV 480

Query: 341 CASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFIVSVQSKVNEVKA 400
             S LI+D L  L    TE D+  IL +L+  G  +R DD  ++K  I   Q+K +   +
Sbjct: 481 VQSLLIFDILKKLIGTFTEKDIELILLMLKNVGFSLRKDDALSLKELITEAQTKASGAGS 540

Query: 401 ASRDDQQNINGKRMEFMLETICDIKNN--KKKPKDDPANHTRIKKWLHKL----GVEDII 460
             +D        R+ FMLET+  +KNN  +K P  DP    +++K    L    G     
Sbjct: 541 EFQDQ------TRIRFMLETMLALKNNDMRKIPGYDPEPVEKLRKLQRALVRNAGSGSET 600

Query: 461 IRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVL---EAQKMLQLAAAQRM 520
              + W  +L  ++ G+WW+ G   + +  I     T   + L    + K+L+LA  QRM
Sbjct: 601 QLRVSWDSVLSAEQTGRWWIVGSAWSGAPMIDNSHHTHLQKQLVGTVSSKILELARKQRM 660

Query: 521 NTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYTILA 580
           NTD R+ IFC IM+ +D++DAFEKLL+L L  +Q+REI+ VL+DCCLQEK +N +Y  LA
Sbjct: 661 NTDIRRNIFCTIMTSEDFLDAFEKLLKLGLKDQQEREIIHVLMDCCLQEKTYNPFYAFLA 720

Query: 581 SKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSVDLS 640
           SK CE+E+  + T Q+ +WD F++LE +      NL    A ++ + +LSL++LK V+ S
Sbjct: 721 SKFCEYERRFQMTFQFSIWDKFRDLENLPATNFSNLVHLVAHLLKTKSLSLSILKVVEFS 780

Query: 641 DIMLLTPKRIMHFRMMFD-SIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYMIK 662
           +   L   R+   R +    + E   + +   FTRV+ NP+L  LR G+  FI  +++K
Sbjct: 781 E---LDKPRVRFLRKVLSILLMETEVEDLSLIFTRVSDNPKLGVLREGLKLFISHFLLK 827

BLAST of CmaCh07G011540 vs. ExPASy Swiss-Prot
Match: Q3UFM5 (Nucleolar MIF4G domain-containing protein 1 OS=Mus musculus OX=10090 GN=Nom1 PE=1 SV=2)

HSP 1 Score: 240.4 bits (612), Expect = 6.2e-62
Identity = 195/663 (29.41%), Postives = 334/663 (50.38%), Query Frame = 0

Query: 41  EEDLELERKFAQKLKVKAGKLRG---------VEDGMNVLFEGLP-----SIVDSPGEDE 100
           EED E+ RK  + L +   K +G           DG++ +   L       + +S  E+E
Sbjct: 185 EEDREI-RKLERCLGLHKRKKKGDGSSVPLSFARDGLDYILGALECGSGGGLYESSEEEE 244

Query: 101 LPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPESEIEKASVGD------GEVEETLEKSK 160
               ++   G++     L    K+    E+ P+ ++ +    D      GE E     +K
Sbjct: 245 ---EEKLETGQTVLESDLESNSKES---EEDPDWQVLQEDQEDVNSKRRGEAESGTRGNK 304

Query: 161 KRKK----KMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIAPNLR------LHKGTE 220
             KK    ++  + + +++D +E   +  VE        +KYI P LR      +HK   
Sbjct: 305 GTKKVRFAEVVEKSRSSSEDDIEQQESHSVE------SGEKYIPPRLRNEEVIDVHK--- 364

Query: 221 PEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEVLASCSRGPRG 280
            E+  +L++ V+GL+NRLSE N+ S++G++  +Y + SR   +  ++  ++ +C      
Sbjct: 365 KEELDRLKKHVKGLINRLSEPNMASISGQLEELYMAHSRKDMNDTLTTALMDACVTASAM 424

Query: 281 NEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRK-EDNLSLRNLTLLLSYLCV 340
             +        V+ +   VG +  A  + ++   F+  YR   +   L NL  ++++L  
Sbjct: 425 PSRLMMEHVFLVSILHHTVGIEVGACFLEAVVKKFDAIYRDGGEGKELDNLFTMIAHLYN 484

Query: 341 FEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFIVSVQSKVNE 400
           F    S LI+D L  L    TE D+  IL +L+  G  +R DD  ++K  I   Q++ + 
Sbjct: 485 FHVVQSILIFDILKKLVGTFTEKDIELILLMLKNVGFALRKDDALSLKELITEAQTQAS- 544

Query: 401 VKAASRDDQQNINGKRMEFMLETICDIKNN--KKKPKDDPANHTRIKKWLHKL----GVE 460
             A ++   QN    R+ FMLET+  +KNN  +K P  +P    ++KK    L    G  
Sbjct: 545 -GAGNKFQDQN----RVRFMLETMLALKNNDLRKIPGYNPEPVEKLKKLQRTLVRNAGSG 604

Query: 461 DIIIRGIKWKKLLDPDKKGQWWLTGDVMTTS---ENITEFASTIDTEVLEAQKMLQLAAA 520
                 I W  +L+ ++ G+WW+ G   + +   +N            + + KML+LA  
Sbjct: 605 SETRLRISWDGILNAEQTGRWWIVGSAWSGTPMIDNSHHIQLQKPLAGMASSKMLELARK 664

Query: 521 QRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYT 580
           QRMNTD R+ IFC +M+ +D++DAFEKLL+L L  +Q+REI+ +L+DCCLQEK +N +Y 
Sbjct: 665 QRMNTDVRRIIFCTLMTSEDFLDAFEKLLKLGLKDQQEREIVHILMDCCLQEKTYNPFYA 724

Query: 581 ILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSV 640
            LASK C++E+  + T Q+ +WD F++LE +   +  NL    A ++ + +L L+VLK V
Sbjct: 725 FLASKFCDYERRFQMTFQFSIWDKFRDLENLPDTKFSNLVHLLAHLLRTKSLPLSVLKVV 784

Query: 641 DLSDIMLLTPKRIMHF--RMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEY 662
           + S++     K  +HF  R++   + E  D  +   F+RV+ NP+L  LR G+  FI  +
Sbjct: 785 EFSEL----DKPRVHFLRRVLTALLMETEDDDLAVIFSRVSDNPKLGMLREGLKLFIGHF 821

BLAST of CmaCh07G011540 vs. ExPASy Swiss-Prot
Match: Q9W020 (Nucleolar MIF4G domain-containing protein 1 homolog OS=Drosophila melanogaster OX=7227 GN=CG9004 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.6e-49
Identity = 183/713 (25.67%), Postives = 334/713 (46.84%), Query Frame = 0

Query: 8   KEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAG--KLRGVE 67
           +++L+ +   ++K+K       +  ++  R+  EED     K  +KLK+     K R V 
Sbjct: 143 RKELRQQKELESKSK-------KQRIKQLRIENEEDDREINKLEKKLKLNKSKDKNRLVR 202

Query: 68  DGMNVLFEGLPSIVDSPGEDELPFSK-EFAVGRSRKNKALNKKEKKRNLLEQSPESEIEK 127
              N   +GL  ++D   +DE    K E    R +K K   +KE+     ++  + E   
Sbjct: 203 KMFN---DGLDYLLDFVLDDEEEKRKWEEKQERKKKLKEQQEKEEAGMWSDEEEDKEDRD 262

Query: 128 ASVGDGEVEETLEKSKKRKKKMSGQHQVTNDDGVESPCAAQVEVQ--------------- 187
             + +   +++  + +   + ++G+ + + +D  +   A +++                 
Sbjct: 263 EPMDNFSEDDSGSEGEDDDEDLTGEEEQSEEDSEQEENAPKIKEDIYGRKRDAEGNILPD 322

Query: 188 ----KLHVKPQKYIAPNLRL-------HKGTEPEDHTQLRRRVRGLLNRLSESNVESVTG 247
               +     QKYI P+ R            + E   +L ++ +GLLNRLSE+N+  +  
Sbjct: 323 PVELEASAAGQKYIPPHQRALMAASAGSSEKQAEILARLLKQCKGLLNRLSEANLHKIAA 382

Query: 248 EMSTVYHSISRSIASQIISEEVLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLM 307
            +  +Y   SR   ++ +++ +  +     R NE+        +A +   VGS+  A  +
Sbjct: 383 GIEELYMKNSRYNMNETLTKLIQEALLGYTRTNERMVQEHMVLLAYLHAQVGSEIGAHFL 442

Query: 308 ASLANTFEEEYR-----KEDNLSLRNLTLLLSYLCVFEACASDLIYDFLIILSKRLTEID 367
            +    F+   +     + ++  L NL L+L Y+ +F+    +L+ + +  LS +L E  
Sbjct: 443 QTFVELFDGYVKNIATLEVEDKQLNNLVLVLCYMYLFKIYELNLLMELIGKLSDQLCEKS 502

Query: 368 VSTILTVLQCCGMKIRGDDPCAMKNFIVSVQSKVNEVKAASRDDQQNINGKRMEFMLETI 427
           V  +L + Q  G ++R DDP A K  +  VQS   ++ +A  + ++N    R+ FM++ +
Sbjct: 503 VECLLLIFQSIGFRLRKDDPLAFKTMMQKVQS---QIASAPLELKEN---PRLRFMVDIL 562

Query: 428 CDIKNN--KKKPKDDPANHTRIKKWLHKLGVED--IIIRGIKWKKLLDPDKKGQWWLTGD 487
             +KNN  +K P+ DP     ++K L  +   D  ++   I  + LL  DK G+WW+ G 
Sbjct: 563 NAVKNNNMQKLPQYDPELAENLRKRLKAMLKNDRYVVTLNITLEDLLRADKVGKWWIVGS 622

Query: 488 VMTTSENITEFASTIDTEVLEAQK---------MLQLAAAQRMNTDARKAIFCIIMSGDD 547
             T   N+ E  S    +   + K         +L+LA  Q+MNT  R+ IFCIIMS  D
Sbjct: 623 AWT--GNLDEMGSAKQKQDKNSSKSANGGFADQLLELARKQQMNTAERRNIFCIIMSAAD 682

Query: 548 YVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYTILASKLCEHEKNHKFTLQYC 607
           YVDAFEK+L L L  K  R +  V++ C L EK  N YY  LA K C+  + ++   Q+ 
Sbjct: 683 YVDAFEKILHLSL--KDQRAVAYVIIHCALNEKRANPYYAHLALKFCQFNRKYQLAFQFA 742

Query: 608 LWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSVDLSDIMLLTPKRIMHF--RM 667
            WD   ++E +   +  NLA F  ++I +  L L+VLK VD   +     K   +F   +
Sbjct: 743 SWDRINDIEKLSKPQIRNLASFLQQVILAAGLQLSVLKVVDFMQL----DKLSFYFMKEV 802

Query: 668 MFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYMIKGNKVNVEKFK 672
           M   +    ++ I+  F RVA N +L+  +  +  F++ ++++  +++  K K
Sbjct: 803 MVRLLLANDEREIYQTFERVAKNTKLQQFKQSVRLFLQHFLLQEEQLDKLKLK 831

BLAST of CmaCh07G011540 vs. ExPASy Swiss-Prot
Match: O13971 (Suppressor of glycerol defect protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sgd1 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 4.5e-44
Identity = 192/719 (26.70%), Postives = 327/719 (45.48%), Query Frame = 0

Query: 8   KEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEED----LELERKFAQKLKVKAGKLRG 67
           K   K +S    + K  + LD  +  Q H+++ EED      LE+    K K K+   + 
Sbjct: 80  KSSTKKKSSQNKQAKESKLLD-PIAFQ-HKIALEEDDREIAHLEKMLGIKSKDKSAHSK- 139

Query: 68  VEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPESEIE 127
           ++     L E L   +D  G   +P +++ + G S  ++  +      ++ E   E E  
Sbjct: 140 IDKEFGWLLEDLDQEIDDIG---VPGTEDPSYGHSEDSEVSDDTGDHGSVDELESERE-- 199

Query: 128 KASVGDGEVEETLEKSKKRKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIAPNL 187
                 GE EE     +    +    HQ         P    V          KY+ P+L
Sbjct: 200 ---GNSGEEEEEFHGFESNSDEF---HQPETKPIRMDPLKPAVPSLPNANVGSKYVPPSL 259

Query: 188 RLHKG--TEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEVL 247
           R   G   E ED  +LRR+++G LN+LS +N+ S+  E+  +Y   SR   +  I+  +L
Sbjct: 260 RKKLGGDKESEDALRLRRKLQGSLNKLSIANISSIIKEIEVLYMENSRHSVTSTITNLLL 319

Query: 248 ASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSL---- 307
            +        +Q A V+AA    +  +VG+DF A L+ +L   F + Y+ ++   L    
Sbjct: 320 QTVMGRESMLDQLAIVYAALATALYRIVGNDFGAHLLQTLVERFLQLYKSKEKEPLSSHK 379

Query: 308 --RNLTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCA 367
              NL +    L  F+  +  L+YD + +  + LTE++V  +L ++  CG ++R DDP  
Sbjct: 380 ETSNLIVFFVELYNFQLVSCVLVYDLIRLFLRSLTELNVEMLLKIVLNCGGQLRSDDP-- 439

Query: 368 MKNFIVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTR--- 427
                 S+Q  V E+         +    R +FM+E+I ++K NKK    + +  ++   
Sbjct: 440 -----TSLQDIVTEMNLLLASADPSTISVRTKFMVESITNLKENKKTKVANASAQSKFEA 499

Query: 428 ---IKKWLHKLGVEDIIIR---GIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDT 487
              +KK+L  LG   +  R    +  + +   + KG+WWL G    +  N+    +T+ T
Sbjct: 500 VNQLKKFLGSLGNRSLNAREPLRVTLEDIEQIETKGRWWLVG---ASWNNVPSGDNTLST 559

Query: 488 EVLEAQ----------KMLQLAAAQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGK 547
           E L+ +          K+LQ A   R+N+  R +IF  ++  +DY+DA+E++L+L L   
Sbjct: 560 EALQDKKKSEELTAHSKILQAAKKLRLNSTLRTSIFVALVGSEDYIDAWERVLKLHLKRN 619

Query: 548 QDREIMRVLVDCCLQEKVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELE------- 607
           Q  EI  V++ C   EK++N +Y ++A K C  + N K + Q+ LWD F EL+       
Sbjct: 620 QLPEIAYVILHCVGNEKLYNPFYGLVALKCCTLQHNLKKSFQFSLWDFFNELQPDDDSEE 679

Query: 608 -TMQLVRSMNLARFTAEMIASYTLSLAVLKSVDLSDIMLLTPKRIMHFRMMF--DSIFER 667
             + + R +NLA+  A ++      L +LK VD   I      ++  F ++F  D I   
Sbjct: 680 REISMRRIVNLAKLYASLVIEAAQPLTILKHVDFMAI----NAQMQTFLLVFFTDIILGV 739

Query: 668 PDKLIWNAFTRVAINPELEP-LRSGMLFFIKEYMIKGNKVNVEKFKVVKKALNNVEGIL 685
            D L      ++  N + E  L S + +F+K Y+ K   V+  K  + K  L     IL
Sbjct: 740 KDDL---QLVKIFENCKAEKNLSSKVDWFLKTYVRKNPLVDNSKKALFKSNLAMASAIL 767

BLAST of CmaCh07G011540 vs. ExPASy Swiss-Prot
Match: Q06132 (Suppressor of glycerol defect protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SGD1 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 8.2e-38
Identity = 137/515 (26.60%), Postives = 236/515 (45.83%), Query Frame = 0

Query: 148 GQHQVTNDDGVESPCAAQVEVQKLHVKP----QKYIAPNLRLHKGTEPEDHT--QLRRRV 207
           G  +  ND   +S    + E +  +V P    + Y+ P+LR        + T  ++ ++V
Sbjct: 279 GLSESDNDSVADSDDNYRREKENPYVAPTQSVESYVPPSLRKKLDDSENNSTLSEISKKV 338

Query: 208 RGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEVLASCSRGPRGNEQYAAVFAAF 267
              LN+LS+SN+  +  +++ +Y S+ R   ++ +++ +L   S+  +  + +   +AA 
Sbjct: 339 NSSLNKLSDSNITIIITDLNRLYDSLPRQYVTESLTKGILNIISQNQKLLDGFIMNYAAL 398

Query: 268 VAGM----GCLVGSDFSARLMASLANTFEEEYR-----KEDNLSLR---NLTLLLSYLCV 327
              +    G  VG+ F  + + +  + +EEE       ++  +S +   N+  LLSY   
Sbjct: 399 AYTLSKLRGIEVGAFFIQKTVEAFLHHYEEEMENILKDQQSKISSKICINIATLLSYCYN 458

Query: 328 FEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFIVSVQSKVNE 387
           F   +  LIYD + I      E     +L ++   G  IRGDDP A+++    +      
Sbjct: 459 FGFVSCRLIYDIIRIFVADPNEFTTELLLRIISISGQLIRGDDPSALRDIRSELLKNAKN 518

Query: 388 VKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHKLGVEDIIIRG 447
           +K  S          R+ F+++T+ D+KNN+ KP     +H  +KK      ++ I+   
Sbjct: 519 LKEQS---------PRLRFLMDTMSDLKNNRLKPSILATDHHPLKK-----NLQSILNSS 578

Query: 448 IKWKKL-------LDPDKKGQWWLTG-DVMTTSENITEFASTIDTEVLEAQK-------- 507
             W+ L        + D KG+WWL G       EN  E +   + +  +++K        
Sbjct: 579 SSWEPLQVSLEDIKNIDSKGKWWLVGASWRGNMENAFEVSINNENDASKSKKSKISIEDD 638

Query: 508 MLQ-------LAAAQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLV 567
           +L        +A  QRMNTD R+AIF  IMS  DY+DAF KL +L L  KQ  EI R+++
Sbjct: 639 LLDDIPDWNIIARQQRMNTDIRRAIFISIMSAQDYLDAFSKLEKLSLKNKQVLEIPRIVL 698

Query: 568 DCCLQE---KVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQ----------- 601
            C L +     +N YY ++A+K+CE   +   + Q+  WD  K+ E  +           
Sbjct: 699 HCLLADSGSNGYNHYYALVANKICERYSHLSKSFQFLFWDVIKKFEDKEFDSESDTDEED 758

BLAST of CmaCh07G011540 vs. ExPASy TrEMBL
Match: A0A6J1HR10 (nucleolar MIF4G domain-containing protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465951 PE=4 SV=1)

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 685/685 (100.00%), Postives = 685/685 (100.00%), Query Frame = 0

Query: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60
           MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK
Sbjct: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60

Query: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120
           LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES
Sbjct: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120

Query: 121 EIEKASVGDGEVEETLEKSKKRKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIA 180
           EIEKASVGDGEVEETLEKSKKRKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIA
Sbjct: 121 EIEKASVGDGEVEETLEKSKKRKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIA 180

Query: 181 PNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEV 240
           PNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEV
Sbjct: 181 PNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEV 240

Query: 241 LASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRNL 300
           LASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRNL
Sbjct: 241 LASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRNL 300

Query: 301 TLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFI 360
           TLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFI
Sbjct: 301 TLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFI 360

Query: 361 VSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHKL 420
           VSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHKL
Sbjct: 361 VSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHKL 420

Query: 421 GVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAAA 480
           GVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAAA
Sbjct: 421 GVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAAA 480

Query: 481 QRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYT 540
           QRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYT
Sbjct: 481 QRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYT 540

Query: 541 ILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSV 600
           ILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSV
Sbjct: 541 ILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSV 600

Query: 601 DLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYMI 660
           DLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYMI
Sbjct: 601 DLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYMI 660

Query: 661 KGNKVNVEKFKVVKKALNNVEGILM 686
           KGNKVNVEKFKVVKKALNNVEGILM
Sbjct: 661 KGNKVNVEKFKVVKKALNNVEGILM 685

BLAST of CmaCh07G011540 vs. ExPASy TrEMBL
Match: A0A6J1HT28 (nucleolar MIF4G domain-containing protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465950 PE=4 SV=1)

HSP 1 Score: 1297.3 bits (3356), Expect = 0.0e+00
Identity = 675/686 (98.40%), Postives = 680/686 (99.13%), Query Frame = 0

Query: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60
           MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK
Sbjct: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60

Query: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120
           LRGVEDGMNVLFEGLPSIVDSPGEDELPFS+EFAVGRSRKNKALNKKEKKR+L EQSPES
Sbjct: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSQEFAVGRSRKNKALNKKEKKRSLSEQSPES 120

Query: 121 EIEKASVGDGEVEETLEKSKKR-KKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI 180
           EIEKASVGDGEVEET EKSKKR KKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI
Sbjct: 121 EIEKASVGDGEVEETSEKSKKRKKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI 180

Query: 181 APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240
           APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE
Sbjct: 181 APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240

Query: 241 VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRN 300
           VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARL+ASLANTFEEEYRKEDNLSLRN
Sbjct: 241 VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLVASLANTFEEEYRKEDNLSLRN 300

Query: 301 LTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360
           LTLLLSYLCVFEACASDLIYDFL ILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF
Sbjct: 301 LTLLLSYLCVFEACASDLIYDFLTILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360

Query: 361 IVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420
           I SVQSKVNEVKAAS DDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK
Sbjct: 361 IASVQSKVNEVKAASGDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420

Query: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAA 480
           LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAA
Sbjct: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAA 480

Query: 481 AQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540
           AQRMNTDARKAIFCI+MSG+DYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY
Sbjct: 481 AQRMNTDARKAIFCIVMSGEDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540

Query: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKS 600
           TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKS
Sbjct: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKS 600

Query: 601 VDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660
           VDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM
Sbjct: 601 VDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660

Query: 661 IKGNKVNVEKFKVVKKALNNVEGILM 686
           IKGNKVNVEKFKVVKKALNNVEGILM
Sbjct: 661 IKGNKVNVEKFKVVKKALNNVEGILM 686

BLAST of CmaCh07G011540 vs. ExPASy TrEMBL
Match: A0A6J1EB72 (nucleolar MIF4G domain-containing protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111432528 PE=4 SV=1)

HSP 1 Score: 1236.9 bits (3199), Expect = 0.0e+00
Identity = 650/686 (94.75%), Postives = 661/686 (96.36%), Query Frame = 0

Query: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60
           MRTARMGKEDLKLRSRTK KTKFEEYLDMEMGMQNHRLSAEEDLELERK AQKLKVKAGK
Sbjct: 1   MRTARMGKEDLKLRSRTKVKTKFEEYLDMEMGMQNHRLSAEEDLELERKLAQKLKVKAGK 60

Query: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120
           LRG EDGMNVLFEGLPSIVDS  EDEL FS+EFAVGRS+K    NKKEKKR +LEQSPES
Sbjct: 61  LRGAEDGMNVLFEGLPSIVDSTVEDELRFSREFAVGRSKK----NKKEKKRKMLEQSPES 120

Query: 121 EIEKASVGDGEVEETLEKSKKR-KKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI 180
           EIEK SVGDGEVEET +KSKKR KKKMSGQHQVT DDGVESPCAA++EVQKL V PQKYI
Sbjct: 121 EIEKGSVGDGEVEETSKKSKKRKKKKMSGQHQVT-DDGVESPCAARIEVQKLSVTPQKYI 180

Query: 181 APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240
           APNLRLHKG EPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE
Sbjct: 181 APNLRLHKGIEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240

Query: 241 VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRN 300
           VLASCSRGPRGNEQYAAVFAAFVAGM CLVGSDFSARLMASLANTFE+EY KEDNLSLRN
Sbjct: 241 VLASCSRGPRGNEQYAAVFAAFVAGMSCLVGSDFSARLMASLANTFEDEYLKEDNLSLRN 300

Query: 301 LTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360
           LTLLLSYLCVFE CASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF
Sbjct: 301 LTLLLSYLCVFEVCASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360

Query: 361 IVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420
           IVSVQSKVNEVKA S D QQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK
Sbjct: 361 IVSVQSKVNEVKAGSGDGQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420

Query: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAA 480
           LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDV TTSENITEFASTIDTEVLEAQKMLQLAA
Sbjct: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVTTTSENITEFASTIDTEVLEAQKMLQLAA 480

Query: 481 AQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540
           AQRMNTDARKAIFCIIMSG+DYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY
Sbjct: 481 AQRMNTDARKAIFCIIMSGEDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540

Query: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKS 600
           TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLA+FTAEMIAS+TLSLAVLKS
Sbjct: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLAKFTAEMIASFTLSLAVLKS 600

Query: 601 VDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660
           VDLSDIMLLTPKRIMHFRM+FDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM
Sbjct: 601 VDLSDIMLLTPKRIMHFRMLFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660

Query: 661 IKGNKVNVEKFKVVKKALNNVEGILM 686
           IKGNKVNVEKFKVVKKALNNVEGILM
Sbjct: 661 IKGNKVNVEKFKVVKKALNNVEGILM 681

BLAST of CmaCh07G011540 vs. ExPASy TrEMBL
Match: A0A5D3CAM3 (Nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00080 PE=4 SV=1)

HSP 1 Score: 1043.5 bits (2697), Expect = 3.9e-301
Identity = 551/691 (79.74%), Postives = 607/691 (87.84%), Query Frame = 0

Query: 6   MGKEDLKLRSRT--KAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGKLRG 65
           MGKED KLRSRT  K KTKFEEYLDME GM+N  LSAE+DLELERK A+KLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKYKGKTKFEEYLDMETGMRNPGLSAEQDLELERKLAKKLKVKAGKLRG 60

Query: 66  VEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPESEIE 125
            +DG+NVLFEG+PSIVD PGE+   FS+EFAV  ++K K L KK KKR  L+Q+ + E E
Sbjct: 61  EDDGINVLFEGIPSIVDFPGEEVSQFSEEFAVEETKK-KPLGKKGKKRKSLDQALDIESE 120

Query: 126 KASVGDGEVEETLEKSKKRKKKMSGQ-------HQVTNDDG--VESPCAAQVEVQKLHVK 185
             +V + E      K +K+KKK   +        +   D+   VES C  +  V K+  K
Sbjct: 121 LTTVVEEENASKKNKKRKKKKKRISEKLEDVVAEETAGDESMPVESHC-VEAAVDKVPAK 180

Query: 186 PQKYIAPNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQ 245
            QKY+APNLRL KGTEPEDHTQLRRRVRGLLNRLSESN+ESVTGEMSTV+HSISRSIASQ
Sbjct: 181 AQKYVAPNLRLQKGTEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQ 240

Query: 246 IISEEVLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDN 305
           IIS+EVLASCSRGPRGNEQYAAVFA+FVAGMGCLVG+DFSARLMASLA TFE+EY KEDN
Sbjct: 241 IISDEVLASCSRGPRGNEQYAAVFASFVAGMGCLVGTDFSARLMASLAKTFEDEYLKEDN 300

Query: 306 LSLRNLTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPC 365
           LSLRNLTLLLSYLCVF  CASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIR DDP 
Sbjct: 301 LSLRNLTLLLSYLCVFGVCASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRADDPT 360

Query: 366 AMKNFIVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIK 425
           AMK+FIVSVQSKVNE+KAAS +  QNINGKRMEFMLETICDIKNNKK+ KDDPA+HTRIK
Sbjct: 361 AMKSFIVSVQSKVNELKAASGNGSQNINGKRMEFMLETICDIKNNKKRSKDDPAHHTRIK 420

Query: 426 KWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKM 485
           KWL KLGV+DIIIRGIKW KLLDPDKKGQWWL+GD+ TTS+N+ EFA+TID EVLEAQKM
Sbjct: 421 KWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLTTTSDNVEEFANTIDKEVLEAQKM 480

Query: 486 LQLAAAQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKV 545
           LQLAAAQRMNTDARKAIFCIIMSG+DYVDAFEKL+RLDLSGKQDREI+RVLVDCCLQEKV
Sbjct: 481 LQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIVRVLVDCCLQEKV 540

Query: 546 FNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSL 605
           FNKYYT+LASKLCEHEKNHKFTLQYCLWD FKEL+TMQL+RSMNLA+F AEMI S+TLSL
Sbjct: 541 FNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAEMITSFTLSL 600

Query: 606 AVLKSVDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFF 665
           AVLKSVDLSDI LLTPKRIMHFRM+FD+IFERPDKLIWN FTRVA+NPELEPLRSGMLFF
Sbjct: 601 AVLKSVDLSDIRLLTPKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELEPLRSGMLFF 660

Query: 666 IKEYMIKGNKVNVEKFKVVKKALNNVEGILM 686
           IKEY+IK +KVN EK+K++KKALNNVEGILM
Sbjct: 661 IKEYVIKTSKVNAEKYKLIKKALNNVEGILM 689

BLAST of CmaCh07G011540 vs. ExPASy TrEMBL
Match: A0A1S3B470 (nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC103485994 PE=4 SV=1)

HSP 1 Score: 1043.5 bits (2697), Expect = 3.9e-301
Identity = 551/691 (79.74%), Postives = 607/691 (87.84%), Query Frame = 0

Query: 6   MGKEDLKLRSRT--KAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGKLRG 65
           MGKED KLRSRT  K KTKFEEYLDME GM+N  LSAE+DLELERK A+KLKVKAGKLRG
Sbjct: 1   MGKEDAKLRSRTKYKGKTKFEEYLDMETGMRNPGLSAEQDLELERKLAKKLKVKAGKLRG 60

Query: 66  VEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPESEIE 125
            +DG+NVLFEG+PSIVD PGE+   FS+EFAV  ++K K L KK KKR  L+Q+ + E E
Sbjct: 61  EDDGINVLFEGIPSIVDFPGEEVSQFSEEFAVEETKK-KPLGKKGKKRKSLDQALDIESE 120

Query: 126 KASVGDGEVEETLEKSKKRKKKMSGQ-------HQVTNDDG--VESPCAAQVEVQKLHVK 185
             +V + E      K +K+KKK   +        +   D+   VES C  +  V K+  K
Sbjct: 121 LTTVVEEENASKKNKKRKKKKKRISEKLEDVVAEETAGDESMPVESHC-VEAAVDKVPAK 180

Query: 186 PQKYIAPNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQ 245
            QKY+APNLRL KGTEPEDHTQLRRRVRGLLNRLSESN+ESVTGEMSTV+HSISRSIASQ
Sbjct: 181 AQKYVAPNLRLQKGTEPEDHTQLRRRVRGLLNRLSESNIESVTGEMSTVFHSISRSIASQ 240

Query: 246 IISEEVLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDN 305
           IIS+EVLASCSRGPRGNEQYAAVFA+FVAGMGCLVG+DFSARLMASLA TFE+EY KEDN
Sbjct: 241 IISDEVLASCSRGPRGNEQYAAVFASFVAGMGCLVGTDFSARLMASLAKTFEDEYLKEDN 300

Query: 306 LSLRNLTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPC 365
           LSLRNLTLLLSYLCVF  CASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIR DDP 
Sbjct: 301 LSLRNLTLLLSYLCVFGVCASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRADDPT 360

Query: 366 AMKNFIVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIK 425
           AMK+FIVSVQSKVNE+KAAS +  QNINGKRMEFMLETICDIKNNKK+ KDDPA+HTRIK
Sbjct: 361 AMKSFIVSVQSKVNELKAASGNGSQNINGKRMEFMLETICDIKNNKKRSKDDPAHHTRIK 420

Query: 426 KWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKM 485
           KWL KLGV+DIIIRGIKW KLLDPDKKGQWWL+GD+ TTS+N+ EFA+TID EVLEAQKM
Sbjct: 421 KWLQKLGVDDIIIRGIKWNKLLDPDKKGQWWLSGDLTTTSDNVEEFANTIDKEVLEAQKM 480

Query: 486 LQLAAAQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKV 545
           LQLAAAQRMNTDARKAIFCIIMSG+DYVDAFEKL+RLDLSGKQDREI+RVLVDCCLQEKV
Sbjct: 481 LQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLIRLDLSGKQDREIVRVLVDCCLQEKV 540

Query: 546 FNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSL 605
           FNKYYT+LASKLCEHEKNHKFTLQYCLWD FKEL+TMQL+RSMNLA+F AEMI S+TLSL
Sbjct: 541 FNKYYTVLASKLCEHEKNHKFTLQYCLWDQFKELDTMQLIRSMNLAKFVAEMITSFTLSL 600

Query: 606 AVLKSVDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFF 665
           AVLKSVDLSDI LLTPKRIMHFRM+FD+IFERPDKLIWN FTRVA+NPELEPLRSGMLFF
Sbjct: 601 AVLKSVDLSDIRLLTPKRIMHFRMLFDAIFERPDKLIWNVFTRVAVNPELEPLRSGMLFF 660

Query: 666 IKEYMIKGNKVNVEKFKVVKKALNNVEGILM 686
           IKEY+IK +KVN EK+K++KKALNNVEGILM
Sbjct: 661 IKEYVIKTSKVNAEKYKLIKKALNNVEGILM 689

BLAST of CmaCh07G011540 vs. NCBI nr
Match: XP_022966215.1 (nucleolar MIF4G domain-containing protein 1-like [Cucurbita maxima])

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 685/685 (100.00%), Postives = 685/685 (100.00%), Query Frame = 0

Query: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60
           MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK
Sbjct: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60

Query: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120
           LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES
Sbjct: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120

Query: 121 EIEKASVGDGEVEETLEKSKKRKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIA 180
           EIEKASVGDGEVEETLEKSKKRKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIA
Sbjct: 121 EIEKASVGDGEVEETLEKSKKRKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYIA 180

Query: 181 PNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEV 240
           PNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEV
Sbjct: 181 PNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEEV 240

Query: 241 LASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRNL 300
           LASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRNL
Sbjct: 241 LASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRNL 300

Query: 301 TLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFI 360
           TLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFI
Sbjct: 301 TLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFI 360

Query: 361 VSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHKL 420
           VSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHKL
Sbjct: 361 VSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHKL 420

Query: 421 GVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAAA 480
           GVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAAA
Sbjct: 421 GVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAAA 480

Query: 481 QRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYT 540
           QRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYT
Sbjct: 481 QRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYT 540

Query: 541 ILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSV 600
           ILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSV
Sbjct: 541 ILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSV 600

Query: 601 DLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYMI 660
           DLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYMI
Sbjct: 601 DLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYMI 660

Query: 661 KGNKVNVEKFKVVKKALNNVEGILM 686
           KGNKVNVEKFKVVKKALNNVEGILM
Sbjct: 661 KGNKVNVEKFKVVKKALNNVEGILM 685

BLAST of CmaCh07G011540 vs. NCBI nr
Match: XP_022966213.1 (nucleolar MIF4G domain-containing protein 1-like [Cucurbita maxima] >XP_022966214.1 nucleolar MIF4G domain-containing protein 1-like [Cucurbita maxima])

HSP 1 Score: 1297.3 bits (3356), Expect = 0.0e+00
Identity = 675/686 (98.40%), Postives = 680/686 (99.13%), Query Frame = 0

Query: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60
           MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK
Sbjct: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60

Query: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120
           LRGVEDGMNVLFEGLPSIVDSPGEDELPFS+EFAVGRSRKNKALNKKEKKR+L EQSPES
Sbjct: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSQEFAVGRSRKNKALNKKEKKRSLSEQSPES 120

Query: 121 EIEKASVGDGEVEETLEKSKKR-KKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI 180
           EIEKASVGDGEVEET EKSKKR KKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI
Sbjct: 121 EIEKASVGDGEVEETSEKSKKRKKKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI 180

Query: 181 APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240
           APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE
Sbjct: 181 APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240

Query: 241 VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRN 300
           VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARL+ASLANTFEEEYRKEDNLSLRN
Sbjct: 241 VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLVASLANTFEEEYRKEDNLSLRN 300

Query: 301 LTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360
           LTLLLSYLCVFEACASDLIYDFL ILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF
Sbjct: 301 LTLLLSYLCVFEACASDLIYDFLTILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360

Query: 361 IVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420
           I SVQSKVNEVKAAS DDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK
Sbjct: 361 IASVQSKVNEVKAASGDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420

Query: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAA 480
           LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAA
Sbjct: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAA 480

Query: 481 AQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540
           AQRMNTDARKAIFCI+MSG+DYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY
Sbjct: 481 AQRMNTDARKAIFCIVMSGEDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540

Query: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKS 600
           TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKS
Sbjct: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKS 600

Query: 601 VDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660
           VDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM
Sbjct: 601 VDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660

Query: 661 IKGNKVNVEKFKVVKKALNNVEGILM 686
           IKGNKVNVEKFKVVKKALNNVEGILM
Sbjct: 661 IKGNKVNVEKFKVVKKALNNVEGILM 686

BLAST of CmaCh07G011540 vs. NCBI nr
Match: XP_023517488.1 (nucleolar MIF4G domain-containing protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1254.2 bits (3244), Expect = 0.0e+00
Identity = 656/686 (95.63%), Postives = 668/686 (97.38%), Query Frame = 0

Query: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60
           MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERK AQKLKVK+GK
Sbjct: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKLAQKLKVKSGK 60

Query: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120
           LRGVEDGMNVLFEGLPSIVDSPGEDELPFS+EFAVGRS+K    NKKEKKR +LEQS ES
Sbjct: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSEEFAVGRSKK----NKKEKKRKMLEQSQES 120

Query: 121 EIEKASVGDGEVEETLEKSKKR-KKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI 180
           EIEKASVGDGEVEET +KSKKR KKKMSGQHQVT DDGVESPCAA++EVQKL V PQKYI
Sbjct: 121 EIEKASVGDGEVEETSKKSKKRKKKKMSGQHQVTADDGVESPCAARIEVQKLPVTPQKYI 180

Query: 181 APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240
           APNLRLHKG EPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE
Sbjct: 181 APNLRLHKGIEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240

Query: 241 VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRN 300
           VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFE+EY KEDNLSLRN
Sbjct: 241 VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEDEYLKEDNLSLRN 300

Query: 301 LTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360
           LTLLLSYLCVFE CASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF
Sbjct: 301 LTLLLSYLCVFEVCASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360

Query: 361 IVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420
           IVSVQSKVNEVKAAS D QQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK
Sbjct: 361 IVSVQSKVNEVKAASGDGQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420

Query: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAA 480
           LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDV TTSENITEFASTIDTEVLEAQKMLQLAA
Sbjct: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVTTTSENITEFASTIDTEVLEAQKMLQLAA 480

Query: 481 AQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540
           AQRMNTDARKAIFCIIMSG+DYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY
Sbjct: 481 AQRMNTDARKAIFCIIMSGEDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540

Query: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKS 600
           TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLA+FTAEMIAS+TLSLAVLKS
Sbjct: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLAKFTAEMIASFTLSLAVLKS 600

Query: 601 VDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660
           VDLSDIMLLTPKRIMHFRM+FDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM
Sbjct: 601 VDLSDIMLLTPKRIMHFRMLFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660

Query: 661 IKGNKVNVEKFKVVKKALNNVEGILM 686
           IKGNKVNVEKFKVVKKALNNVEGILM
Sbjct: 661 IKGNKVNVEKFKVVKKALNNVEGILM 682

BLAST of CmaCh07G011540 vs. NCBI nr
Match: KAG7027482.1 (Nucleolar MIF4G domain-containing protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1238.8 bits (3204), Expect = 0.0e+00
Identity = 654/693 (94.37%), Postives = 665/693 (95.96%), Query Frame = 0

Query: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60
           MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERK AQKLKVKAGK
Sbjct: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKLAQKLKVKAGK 60

Query: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120
           LRG EDGMNVLFEGLPSIVDS  EDELP S+EFAVGRS+K    NKKEKKRN+LEQSPES
Sbjct: 61  LRGAEDGMNVLFEGLPSIVDSTVEDELPLSREFAVGRSKK----NKKEKKRNMLEQSPES 120

Query: 121 EIEKASVGDGEVEETLEKSKKR-KKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI 180
           EIEKASVGDGEVEET +KSKKR KKKMSGQHQVT DDGVESPCAA++EVQKL V PQKYI
Sbjct: 121 EIEKASVGDGEVEETSKKSKKRKKKKMSGQHQVT-DDGVESPCAARIEVQKLSVAPQKYI 180

Query: 181 APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240
           APNLRLHKG EPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE
Sbjct: 181 APNLRLHKGIEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240

Query: 241 VLASCSRGPRGNEQ-------YAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKE 300
           VLASCSRGPRGNEQ       YAAVFAAFVAGMGCLVGSDFSARLMASLANTFE+EY KE
Sbjct: 241 VLASCSRGPRGNEQLSVCLILYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEDEYLKE 300

Query: 301 DNLSLRNLTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDD 360
           DNLSLRNLTLLLSYLCVFE CASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDD
Sbjct: 301 DNLSLRNLTLLLSYLCVFEVCASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDD 360

Query: 361 PCAMKNFIVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTR 420
           PCAMKNFIVSVQSKVNEVKA S D QQNINGKRMEFMLETICDIKNNKKKPKDDPANHTR
Sbjct: 361 PCAMKNFIVSVQSKVNEVKAGSGDGQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTR 420

Query: 421 IKKWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQ 480
           IKKWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDV TTSENITEFASTIDTEVLEAQ
Sbjct: 421 IKKWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVTTTSENITEFASTIDTEVLEAQ 480

Query: 481 KMLQLAAAQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQE 540
           KMLQLAAAQRMNTDARKAIFCIIMSG+DYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQE
Sbjct: 481 KMLQLAAAQRMNTDARKAIFCIIMSGEDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQE 540

Query: 541 KVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTL 600
           KVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLA+FTAEMIAS+TL
Sbjct: 541 KVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLAKFTAEMIASFTL 600

Query: 601 SLAVLKSVDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGML 660
           SLAVLKSVDLSDIMLLTPKRIMHFRM+FDSIFERPDKLIWNAFTRVAINPELEPLRSGML
Sbjct: 601 SLAVLKSVDLSDIMLLTPKRIMHFRMLFDSIFERPDKLIWNAFTRVAINPELEPLRSGML 660

Query: 661 FFIKEYMIKGNKVNVEKFKVVKKALNNVEGILM 686
           FFIKEYMIKGNKVNVEKFKVVKKALNNVEGILM
Sbjct: 661 FFIKEYMIKGNKVNVEKFKVVKKALNNVEGILM 688

BLAST of CmaCh07G011540 vs. NCBI nr
Match: XP_022925222.1 (nucleolar MIF4G domain-containing protein 1 [Cucurbita moschata])

HSP 1 Score: 1236.9 bits (3199), Expect = 0.0e+00
Identity = 650/686 (94.75%), Postives = 661/686 (96.36%), Query Frame = 0

Query: 1   MRTARMGKEDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGK 60
           MRTARMGKEDLKLRSRTK KTKFEEYLDMEMGMQNHRLSAEEDLELERK AQKLKVKAGK
Sbjct: 1   MRTARMGKEDLKLRSRTKVKTKFEEYLDMEMGMQNHRLSAEEDLELERKLAQKLKVKAGK 60

Query: 61  LRGVEDGMNVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRNLLEQSPES 120
           LRG EDGMNVLFEGLPSIVDS  EDEL FS+EFAVGRS+K    NKKEKKR +LEQSPES
Sbjct: 61  LRGAEDGMNVLFEGLPSIVDSTVEDELRFSREFAVGRSKK----NKKEKKRKMLEQSPES 120

Query: 121 EIEKASVGDGEVEETLEKSKKR-KKKMSGQHQVTNDDGVESPCAAQVEVQKLHVKPQKYI 180
           EIEK SVGDGEVEET +KSKKR KKKMSGQHQVT DDGVESPCAA++EVQKL V PQKYI
Sbjct: 121 EIEKGSVGDGEVEETSKKSKKRKKKKMSGQHQVT-DDGVESPCAARIEVQKLSVTPQKYI 180

Query: 181 APNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240
           APNLRLHKG EPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE
Sbjct: 181 APNLRLHKGIEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTVYHSISRSIASQIISEE 240

Query: 241 VLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLANTFEEEYRKEDNLSLRN 300
           VLASCSRGPRGNEQYAAVFAAFVAGM CLVGSDFSARLMASLANTFE+EY KEDNLSLRN
Sbjct: 241 VLASCSRGPRGNEQYAAVFAAFVAGMSCLVGSDFSARLMASLANTFEDEYLKEDNLSLRN 300

Query: 301 LTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360
           LTLLLSYLCVFE CASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF
Sbjct: 301 LTLLLSYLCVFEVCASDLIYDFLIILSKRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNF 360

Query: 361 IVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420
           IVSVQSKVNEVKA S D QQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK
Sbjct: 361 IVSVQSKVNEVKAGSGDGQQNINGKRMEFMLETICDIKNNKKKPKDDPANHTRIKKWLHK 420

Query: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAA 480
           LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDV TTSENITEFASTIDTEVLEAQKMLQLAA
Sbjct: 421 LGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVTTTSENITEFASTIDTEVLEAQKMLQLAA 480

Query: 481 AQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540
           AQRMNTDARKAIFCIIMSG+DYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY
Sbjct: 481 AQRMNTDARKAIFCIIMSGEDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYY 540

Query: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKS 600
           TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLA+FTAEMIAS+TLSLAVLKS
Sbjct: 541 TILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLAKFTAEMIASFTLSLAVLKS 600

Query: 601 VDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660
           VDLSDIMLLTPKRIMHFRM+FDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM
Sbjct: 601 VDLSDIMLLTPKRIMHFRMLFDSIFERPDKLIWNAFTRVAINPELEPLRSGMLFFIKEYM 660

Query: 661 IKGNKVNVEKFKVVKKALNNVEGILM 686
           IKGNKVNVEKFKVVKKALNNVEGILM
Sbjct: 661 IKGNKVNVEKFKVVKKALNNVEGILM 681

BLAST of CmaCh07G011540 vs. TAIR 10
Match: AT5G17930.1 (MIF4G domain-containing protein / MA3 domain-containing protein )

HSP 1 Score: 736.1 bits (1899), Expect = 2.6e-212
Identity = 393/702 (55.98%), Postives = 522/702 (74.36%), Query Frame = 0

Query: 9   EDLKLRSRTKAKTKFEEYLDMEMGMQNHRLSAEEDLELERKFAQKLKVKAGKLRGVEDGM 68
           E +K  +R + KT FEE+L+M+       +S ++D ELER+ A+KLK+K GKLRG++DG+
Sbjct: 100 ERIKDLNRPRKKTNFEEFLEMDTPTV---ISGDQDAELERRLAKKLKIKKGKLRGMDDGL 159

Query: 69  NVLFEGLPSIVDSPGEDELPFSKEFAVGRSRKNKALNKKEKKRN------LLEQSPESEI 128
           N LFEGLPS++DS G +         +G SRK +   + E+K++      L  +  E E 
Sbjct: 160 NDLFEGLPSVLDSMGSE---------LGDSRKKRKKKRSEEKQDHEDVDELANEDLEHEE 219

Query: 129 EKASVGDGEVEETLEKSKKR--KKKMSGQHQVTN------DDGV-------ESPCAAQVE 188
            + S  + E E   ++ +KR  KKK S   ++ +      DDG        +SP + +  
Sbjct: 220 SEFSDEESEEEPVGKRDRKRHKKKKKSVDEELESDLMNITDDGESETVNYHDSPSSLEKV 279

Query: 189 VQKLHV-KPQ---KYIAPNLRLHKGTEPEDHTQLRRRVRGLLNRLSESNVESVTGEMSTV 248
              LH  KP+   KY+AP+LR    +E E+ T++R R++GLLN+++ESNVE++T E++T+
Sbjct: 280 DTPLHERKPESSSKYVAPHLRSQAKSESEELTKMRTRIKGLLNKMAESNVETITAELATI 339

Query: 249 YHSISRSIASQIISEEVLASCSRGPRGNEQYAAVFAAFVAGMGCLVGSDFSARLMASLAN 308
           Y S++RS++SQI  EEVL + +   RGNEQY AVFA+F+AGM CLVG DFSA+L+ASLA 
Sbjct: 340 YRSVARSVSSQIFCEEVLTTYA---RGNEQY-AVFASFIAGMACLVGMDFSAKLIASLAK 399

Query: 309 TFEEEYRKEDNLSLRNLTLLLSYLCVFEACASDLIYDFLIILSKRLTEIDVSTILTVLQC 368
           +FE+EY+KED+LSL  ++LLLSYLC+   C+SDLIYDFL+ L KRLT++D  TI TVL C
Sbjct: 400 SFEDEYQKEDSLSLNGISLLLSYLCILGVCSSDLIYDFLMTLGKRLTKVDAFTITTVLDC 459

Query: 369 CGMKIRGDDPCAMKNFIVSVQSKVNEVKAASRDDQQNINGKRMEFMLETICDIKNNKKKP 428
           CGMKIR DDP AMK FI+S+Q+K NE+K +S D Q  +N   ME MLETI  IKNNK + 
Sbjct: 460 CGMKIRSDDPLAMKTFIISIQNKANELKTSS-DGQTQMNNMMMEKMLETISAIKNNKLRA 519

Query: 429 KDDPANHTRIKKWLHKLGVEDIIIRGIKWKKLLDPDKKGQWWLTGDVMTTSENITEFAST 488
           K+D   +TR+KKWL KL VE++++RG+ W KLLDP+KKGQWWL+GD++  S    + A T
Sbjct: 520 KEDSVQNTRVKKWLQKLRVEEVLLRGLTWSKLLDPEKKGQWWLSGDLVVDSNTAEDIAET 579

Query: 489 IDTEVLEAQKMLQLAAAQRMNTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMR 548
           +D EV+EAQKML+LA AQRMNTD+RKAIFC+IMS +DY+DAFEKLLRLDL GKQDREIMR
Sbjct: 580 MDAEVVEAQKMLKLAEAQRMNTDSRKAIFCVIMSSEDYIDAFEKLLRLDLPGKQDREIMR 639

Query: 549 VLVDCCLQEKVFNKYYTILASKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFT 608
           VLV+CCLQEK FNK+YT+LASKLCEH+KNHKFTLQYC+WDHFKELE+M L RSM+LA+F 
Sbjct: 640 VLVECCLQEKAFNKFYTVLASKLCEHDKNHKFTLQYCIWDHFKELESMSLQRSMHLAKFV 699

Query: 609 AEMIASYTLSLAVLKSVDLSDIMLLTPKRIMHFRMMFDSIFERPDKLIWNAFTRVAINPE 668
           AE+I ++ LSLAVLKSVDL++ + LTPKRIMHFRM+F++IFE P+ L+WN FTR+A+NPE
Sbjct: 700 AEIIVTFNLSLAVLKSVDLANPVELTPKRIMHFRMLFEAIFEHPENLVWNLFTRIALNPE 759

Query: 669 LEPLRSGMLFFIKEYMIKGNKVNVEKFKVVKKALNNVEGILM 686
            E LR G+ FF+KEY++K NK    KF+  K+ALNN EG+LM
Sbjct: 760 YEALRDGIKFFVKEYVVKNNKAIYGKFRKAKEALNNAEGLLM 784

BLAST of CmaCh07G011540 vs. TAIR 10
Match: AT1G80930.1 (MIF4G domain-containing protein / MA3 domain-containing protein )

HSP 1 Score: 65.9 bits (159), Expect = 1.5e-10
Identity = 111/562 (19.75%), Postives = 235/562 (41.81%), Query Frame = 0

Query: 97  RSRKNKALNKKEKKRNLLEQSPESEIEKASVGDGEVEETLEKSKKRKKKMSGQHQVTNDD 156
           RSR++  + + EK+R   ++  +S+ +K    D        + ++ KK    + ++   +
Sbjct: 266 RSRRDDGV-RDEKERRHNDKYDDSQRDKLRKED-------SRKREEKKIEVPKPKLAELN 325

Query: 157 GVESPCAAQVEVQKLHVKPQKYIAPNLRLHKGTEPEDHTQ--------LRRRVRGLLNRL 216
             E+   A  +   +++ P K      R+ K  E +   +        LR+ + GL+N++
Sbjct: 326 PSENNAMALGKTGGVYIPPFKL----ARMMKEVEDKSSVEYQRLTWDALRKSINGLVNKV 385

Query: 217 SESNVESVTGEMSTVYHSISRSIASQIISEEVL--ASCSRGPRGNEQYAAVFAAFVAGMG 276
           + SN++++  E+           A  +I    L   SC +    +  +  VFAA VA + 
Sbjct: 386 NASNIKNIIPEL----------FAENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVIN 445

Query: 277 CLVGSDFSARLMASLANTFEEEYRKEDNLSLRNLTLLLSYLCVFEACASDLIYDFLIILS 336
                + +  L+  +    +  Y++ D   L      +++L   +     +  + + IL 
Sbjct: 446 AKF-PEVAELLLKRVVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAEEIIALELVTILL 505

Query: 337 KRLTEIDVSTILTVLQCCGMKIRGDDPCAMKNFIVSVQSKVNEVKAASRDDQQNINGKRM 396
              T+  V   +  +  CG  ++   P  +       +  ++E +            KR+
Sbjct: 506 GDPTDDSVEVAVGFVTECGAMLQDVSPRGLNGIFERFRGILHEGEI----------DKRV 565

Query: 397 EFMLETICDIKNNKKKPKDDPANHTRI----KKWLHKLGVE----------------DII 456
           ++++E++      K K +  PA    +    +K+ H L ++                D +
Sbjct: 566 QYLIESL--FATRKAKFQGHPAVRPELDLVEEKYSHDLSLDHEIDPETALDIFKPDPDFV 625

Query: 457 IRGIKW---KKLLDPDKKGQWWLTGDVMTTSENITEFASTIDTEVLEAQKMLQLAAAQRM 516
               K+   KK L  D++ +     D   +SE+  E     D E  E  ++        +
Sbjct: 626 ENEKKYEALKKELLGDEESEDEDGSD--ASSEDNDEEEDESDEEDEEQMRIRDETETNLV 685

Query: 517 NTDARKAIFCIIMSGDDYVDAFEKLLRLDLSGKQDREIMRVLVDCCLQEKVFNKYYTILA 576
           N   R+ I+  IMS  D+ +A  KLL++ L   Q+ E+  +L++CC QE+ + +YY +L 
Sbjct: 686 N--LRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEMELCIMLLECCSQERTYLRYYGLLG 745

Query: 577 SKLCEHEKNHKFTLQYCLWDHFKELETMQLVRSMNLARFTAEMIASYTLSLAVLKSVDLS 626
            + C   K H+   + C    +  +  ++  +  N+A+F A ++ +  L   VL  + L+
Sbjct: 746 QRFCMINKIHQENFEKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLAYIRLT 787

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5C9Z43.5e-6530.50Nucleolar MIF4G domain-containing protein 1 OS=Homo sapiens OX=9606 GN=NOM1 PE=1... [more]
Q3UFM56.2e-6229.41Nucleolar MIF4G domain-containing protein 1 OS=Mus musculus OX=10090 GN=Nom1 PE=... [more]
Q9W0201.6e-4925.67Nucleolar MIF4G domain-containing protein 1 homolog OS=Drosophila melanogaster O... [more]
O139714.5e-4426.70Suppressor of glycerol defect protein 1 OS=Schizosaccharomyces pombe (strain 972... [more]
Q061328.2e-3826.60Suppressor of glycerol defect protein 1 OS=Saccharomyces cerevisiae (strain ATCC... [more]
Match NameE-valueIdentityDescription
A0A6J1HR100.0e+00100.00nucleolar MIF4G domain-containing protein 1-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1HT280.0e+0098.40nucleolar MIF4G domain-containing protein 1-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1EB720.0e+0094.75nucleolar MIF4G domain-containing protein 1 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A5D3CAM33.9e-30179.74Nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B4703.9e-30179.74nucleolar MIF4G domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC103485... [more]
Match NameE-valueIdentityDescription
XP_022966215.10.0e+00100.00nucleolar MIF4G domain-containing protein 1-like [Cucurbita maxima][more]
XP_022966213.10.0e+0098.40nucleolar MIF4G domain-containing protein 1-like [Cucurbita maxima] >XP_02296621... [more]
XP_023517488.10.0e+0095.63nucleolar MIF4G domain-containing protein 1 [Cucurbita pepo subsp. pepo][more]
KAG7027482.10.0e+0094.37Nucleolar MIF4G domain-containing protein 1 [Cucurbita argyrosperma subsp. argyr... [more]
XP_022925222.10.0e+0094.75nucleolar MIF4G domain-containing protein 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G17930.12.6e-21255.98MIF4G domain-containing protein / MA3 domain-containing protein [more]
AT1G80930.11.5e-1019.75MIF4G domain-containing protein / MA3 domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003890MIF4G-like, type 3SMARTSM00543if4_15coord: 198..400
e-value: 6.5E-15
score: 65.5
IPR003890MIF4G-like, type 3PFAMPF02854MIF4Gcoord: 199..400
e-value: 2.3E-13
score: 50.3
IPR003891Initiation factor eIF-4 gamma, MA3SMARTSM00544ma3_7coord: 487..593
e-value: 2.1E-17
score: 73.8
IPR003891Initiation factor eIF-4 gamma, MA3PFAMPF02847MA3coord: 488..591
e-value: 4.5E-22
score: 78.2
IPR003891Initiation factor eIF-4 gamma, MA3PROSITEPS51366MIcoord: 486..602
score: 21.393913
IPR016021MIF4G-like domain superfamilyGENE3D1.25.40.180coord: 165..460
e-value: 3.1E-56
score: 192.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..160
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..141
NoneNo IPR availablePANTHERPTHR18034CELL CYCLE CONTROL PROTEIN CWF22-RELATEDcoord: 11..682
NoneNo IPR availablePANTHERPTHR18034:SF4NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1coord: 11..682
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 190..402

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G011540.1CmaCh07G011540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006417 regulation of translation
biological_process GO:0042274 ribosomal small subunit biogenesis
cellular_component GO:0005730 nucleolus
molecular_function GO:0005515 protein binding
molecular_function GO:0003723 RNA binding