CmaCh07G009410 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh07G009410
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Mediator of RNA polymerase II transcription subunit 4
Location	Cma_Chr07: 4526699 .. 4527922 (-)
RNA-Seq Expression	CmaCh07G009410
Synteny	CmaCh07G009410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCAGCATCAAATTGTACAGTCCCCAGCAAGATTGGGCTTGACGAACCCTAATTCTCCTTCATTTCAAAACCCTACCCCACCGAAATTGCCCCCTTCTCAACAATCCCACCACCAATATCAATCCAATCTTTCATCTCCGACGCCCTCCTCCACGCTTCTTCCTCTCCTTCCTCCTCTTCCTAGAGCTCAAGCTCTTCTCCTGCAAATGGCTTCTTTAGCTTCCAAGCTCTTCGAAGTTTACCCCAATCGAGCCCTTTGGCTCACTGCATTTCGGGGCTCTCTTCCCACTTTTCTTAATTCTCAAACCCAGTCGGCTCCCTCTACGCCGCTTGATTCTACTCCGTCTACTTCGAAAGAAATTCTGTCACTGTTTAATGTTCTGCAGACTCAGTTGTTTGAAGCCGTTGCTGAGCTTCAGGAGATTCTTGATCTTCAAGACTCTAAGCAAAAGATTGCTTATGAAATGCGGGCTAAAGATGCTGCTCTTCGTGCTTTTGCTAATAAACTAAAGGATGCTGAGCAGGTTCTTGATGTTTTGTTTGATGATTATGCTGATTATCAGGGTCATAAACGTGTTAAAACGAACGATGATGTCGAAAACGAGTCATTATGTTCGACTACTACTGTTGCGTCCCAGTTGAAATTATCTGATATATTGTCATATGCTCATAAAATAAGCTATACTACTTTTGCACCACCTGAATTTGGGGCTGGACAAGCTCCATTGCGCGGGGCGCTTCCCCCCGCCCCGCAAGAAGAGCAAATGCGTGCTTCCCAGTTGTATAGTTTTGCTGATCTTGATGTTGGCTTGCCTAAATCAGTTGAAAGTACTGAGAAAACCATCGAGCCAATGATTGAGGCACCAGCTCCAGTGCCAACAGATGCCAATCCTTTAGCTCTGGCTGGTATCCAAGGGCTGCTTCCTCCAAATATCACTGTGCCATCTGGTTGGAAGCCTGGAATGCCTGTGGAATTACCTAGAGATTTACCAATGCCCCCTCCTGGATGGAAGCCTGGGGACCCTGTGCCATTACCCCCATTGGACTCTCTTCCTGTTGCTAGAGTTGATGAGCCACAATTGAGGCATAATGCTCTTCCAGGATTACACAGACCTCAAGAGCCAATTCAAGTTAGGCATGTTCAGTTGGATATTCTCGATCAAGACGATGATAGTAGCGATTATGCTAGTGAAGAAGGAAGCTCTGAAGATGACGATTGA

mRNA sequence

ATGCTTCAGCATCAAATTGTACAGTCCCCAGCAAGATTGGGCTTGACGAACCCTAATTCTCCTTCATTTCAAAACCCTACCCCACCGAAATTGCCCCCTTCTCAACAATCCCACCACCAATATCAATCCAATCTTTCATCTCCGACGCCCTCCTCCACGCTTCTTCCTCTCCTTCCTCCTCTTCCTAGAGCTCAAGCTCTTCTCCTGCAAATGGCTTCTTTAGCTTCCAAGCTCTTCGAAGTTTACCCCAATCGAGCCCTTTGGCTCACTGCATTTCGGGGCTCTCTTCCCACTTTTCTTAATTCTCAAACCCAGTCGGCTCCCTCTACGCCGCTTGATTCTACTCCGTCTACTTCGAAAGAAATTCTGTCACTGTTTAATGTTCTGCAGACTCAGTTGTTTGAAGCCGTTGCTGAGCTTCAGGAGATTCTTGATCTTCAAGACTCTAAGCAAAAGATTGCTTATGAAATGCGGGCTAAAGATGCTGCTCTTCGTGCTTTTGCTAATAAACTAAAGGATGCTGAGCAGGTTCTTGATGTTTTGTTTGATGATTATGCTGATTATCAGGGTCATAAACGTGTTAAAACGAACGATGATGTCGAAAACGAGTCATTATGTTCGACTACTACTGTTGCGTCCCAGTTGAAATTATCTGATATATTGTCATATGCTCATAAAATAAGCTATACTACTTTTGCACCACCTGAATTTGGGGCTGGACAAGCTCCATTGCGCGGGGCGCTTCCCCCCGCCCCGCAAGAAGAGCAAATGCGTGCTTCCCAGTTGTATAGTTTTGCTGATCTTGATGTTGGCTTGCCTAAATCAGTTGAAAGTACTGAGAAAACCATCGAGCCAATGATTGAGGCACCAGCTCCAGTGCCAACAGATGCCAATCCTTTAGCTCTGGCTGGTATCCAAGGGCTGCTTCCTCCAAATATCACTGTGCCATCTGGTTGGAAGCCTGGAATGCCTGTGGAATTACCTAGAGATTTACCAATGCCCCCTCCTGGATGGAAGCCTGGGGACCCTGTGCCATTACCCCCATTGGACTCTCTTCCTGTTGCTAGAGTTGATGAGCCACAATTGAGGCATAATGCTCTTCCAGGATTACACAGACCTCAAGAGCCAATTCAAGTTAGGCATGTTCAGTTGGATATTCTCGATCAAGACGATGATAGTAGCGATTATGCTAGTGAAGAAGGAAGCTCTGAAGATGACGATTGA

Coding sequence (CDS)

ATGCTTCAGCATCAAATTGTACAGTCCCCAGCAAGATTGGGCTTGACGAACCCTAATTCTCCTTCATTTCAAAACCCTACCCCACCGAAATTGCCCCCTTCTCAACAATCCCACCACCAATATCAATCCAATCTTTCATCTCCGACGCCCTCCTCCACGCTTCTTCCTCTCCTTCCTCCTCTTCCTAGAGCTCAAGCTCTTCTCCTGCAAATGGCTTCTTTAGCTTCCAAGCTCTTCGAAGTTTACCCCAATCGAGCCCTTTGGCTCACTGCATTTCGGGGCTCTCTTCCCACTTTTCTTAATTCTCAAACCCAGTCGGCTCCCTCTACGCCGCTTGATTCTACTCCGTCTACTTCGAAAGAAATTCTGTCACTGTTTAATGTTCTGCAGACTCAGTTGTTTGAAGCCGTTGCTGAGCTTCAGGAGATTCTTGATCTTCAAGACTCTAAGCAAAAGATTGCTTATGAAATGCGGGCTAAAGATGCTGCTCTTCGTGCTTTTGCTAATAAACTAAAGGATGCTGAGCAGGTTCTTGATGTTTTGTTTGATGATTATGCTGATTATCAGGGTCATAAACGTGTTAAAACGAACGATGATGTCGAAAACGAGTCATTATGTTCGACTACTACTGTTGCGTCCCAGTTGAAATTATCTGATATATTGTCATATGCTCATAAAATAAGCTATACTACTTTTGCACCACCTGAATTTGGGGCTGGACAAGCTCCATTGCGCGGGGCGCTTCCCCCCGCCCCGCAAGAAGAGCAAATGCGTGCTTCCCAGTTGTATAGTTTTGCTGATCTTGATGTTGGCTTGCCTAAATCAGTTGAAAGTACTGAGAAAACCATCGAGCCAATGATTGAGGCACCAGCTCCAGTGCCAACAGATGCCAATCCTTTAGCTCTGGCTGGTATCCAAGGGCTGCTTCCTCCAAATATCACTGTGCCATCTGGTTGGAAGCCTGGAATGCCTGTGGAATTACCTAGAGATTTACCAATGCCCCCTCCTGGATGGAAGCCTGGGGACCCTGTGCCATTACCCCCATTGGACTCTCTTCCTGTTGCTAGAGTTGATGAGCCACAATTGAGGCATAATGCTCTTCCAGGATTACACAGACCTCAAGAGCCAATTCAAGTTAGGCATGTTCAGTTGGATATTCTCGATCAAGACGATGATAGTAGCGATTATGCTAGTGAAGAAGGAAGCTCTGAAGATGACGATTGA

Protein sequence

MLQHQIVQSPARLGLTNPNSPSFQNPTPPKLPPSQQSHHQYQSNLSSPTPSSTLLPLLPPLPRAQALLLQMASLASKLFEVYPNRALWLTAFRGSLPTFLNSQTQSAPSTPLDSTPSTSKEILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEMRAKDAALRAFANKLKDAEQVLDVLFDDYADYQGHKRVKTNDDVENESLCSTTTVASQLKLSDILSYAHKISYTTFAPPEFGAGQAPLRGALPPAPQEEQMRASQLYSFADLDVGLPKSVESTEKTIEPMIEAPAPVPTDANPLALAGIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVARVDEPQLRHNALPGLHRPQEPIQVRHVQLDILDQDDDSSDYASEEGSSEDDD

Homology

[Close]

BLAST of CmaCh07G009410 vs. ExPASy Swiss-Prot
Match: Q9LZ00 (Mediator of RNA polymerase II transcription subunit 4 OS=Arabidopsis thaliana OX=3702 GN=MED4 PE=1 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 6.6e-136
Identity = 280/432 (64.81%), Postives = 328/432 (75.93%), Query Frame = 0


Query: 1   MLQHQIVQSPARLGLTNPNSPSFQNPTP-----PKLPPSQQSHH---QYQSNL------- 60
           MLQHQIVQSPARLGLT P SPS QNPTP     P    S QS H   Q Q NL       
Sbjct: 1   MLQHQIVQSPARLGLTGPGSPSVQNPTPTRHGHPTSSSSSQSQHQQIQQQPNLLPSSTVA 60

Query: 61  -------SSPTPSSTLLPLLPPLPRAQALLLQMASLASKLFEVYPNRALWLTAFRGSLPT 120
                  S+   SS LL LLPPLPRAQALL QMA L SKLF+V PNRA+WL+AFRGSLP+
Sbjct: 61  AASSASASAAVSSSALLSLLPPLPRAQALLQQMAVLTSKLFDVSPNRAIWLSAFRGSLPS 120

Query: 121 FLNSQTQSAPSTPLDSTPSTSKEILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEMR 180
           FL+S +   P    + +PS++KEILS FN LQTQLFEAV ELQEILDLQD+KQK+A E++
Sbjct: 121 FLSSHSLPPPPPLENPSPSSTKEILSQFNSLQTQLFEAVTELQEILDLQDAKQKVAREIK 180

Query: 181 AKDAALRAFANKLKDAEQVLDVLFDDYADYQGHKRVKTNDDVE---NESLCSTTTVASQL 240
           +KD++L AFANKLKDAE+VLD+L DDY+DY+  KR K  +D E   NES  S+TTV+SQL
Sbjct: 181 SKDSSLLAFANKLKDAERVLDMLVDDYSDYRKPKRSKIEEDDEDNDNESSSSSTTVSSQL 240

Query: 241 KLSDILSYAHKISYTTFAPPEFGAGQAPLRGALPPAPQEEQMRASQLYSFADLDVGLPKS 300
           KL DIL+YAHKISYTTFAPPEFGAGQAPLRGALPPAPQ+EQMRASQLY+FADLD+GLPK+
Sbjct: 241 KLKDILAYAHKISYTTFAPPEFGAGQAPLRGALPPAPQDEQMRASQLYTFADLDIGLPKT 300

Query: 301 VESTEKTIEPMIEAPAPVPTDANPLALAGIQGLLPPNITVPSGWKPGMPVELPRDLPMPP 360
           VE+ EK +E +IE P P       + ++ I  LLPPNI VPSGWKPGMPVELPRDLP+PP
Sbjct: 301 VENMEKKVEALIEPPPP----PEAMDISAIHNLLPPNIAVPSGWKPGMPVELPRDLPLPP 360

Query: 361 PGWKPGDPVPLPPLDSLPVARVDEPQLRHNALPGLHRPQEPIQVRHVQLDILDQDDDSSD 408
           PGWKPGDPV LPPL+S+   R ++ Q       GLHRP + IQVR VQLDIL +DDDSSD
Sbjct: 361 PGWKPGDPVVLPPLESIAAPRAEDHQ-HMRPSQGLHRPPDVIQVRAVQLDIL-EDDDSSD 420

[Close]

BLAST of CmaCh07G009410 vs. ExPASy Swiss-Prot
Match: Q54D00 (Putative mediator of RNA polymerase II transcription subunit 4 OS=Dictyostelium discoideum OX=44689 GN=med4 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.9e-05
Identity = 62/202 (30.69%), Postives = 88/202 (43.56%), Query Frame = 0


Query: 100 LNSQTQSAPSTPLD--STPSTSKEILSLFNVL--QTQLFE-AVAELQEILDLQDSKQKIA 159
           LNSQ       P+D  S  +T   +L L N L    ++++  + +L++   +Q    +I 
Sbjct: 44  LNSQQLQQIQLPIDQISNNATPNMVLQLMNHLIDVDKIYQNTLKKLEKHQKIQKEITEIQ 103

Query: 160 YEMRAKDAALRAFANKLKDAEQVLDVLFDDYADYQGHKRVKTNDDVENESLCS------- 219
            E+  KD  +   A  LKD E  L+       D         +D + NE L         
Sbjct: 104 KEIEEKDKLISTLALNLKDIESFLENELSQ--DNVNINNGNASDGIVNEVLIDLKQPDDA 163

Query: 220 ---TTTVASQLKLSDILSYAHKISYTTFAPPEFG-AGQAPLRGAL-PPAPQEEQMRASQL 274
                 V  ++   D++SYAHKIS TT AP  FG    APL     PPAPQ+E MR+S L
Sbjct: 164 ASMDNIVNREVSPEDLISYAHKISGTTSAP--FGYQPNAPLASLFKPPAPQDEMMRSSVL 223

[Close]

BLAST of CmaCh07G009410 vs. TAIR 10
Match: AT5G02850.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 485.3 bits (1248), Expect = 4.7e-137
Identity = 280/432 (64.81%), Postives = 328/432 (75.93%), Query Frame = 0


Query: 1   MLQHQIVQSPARLGLTNPNSPSFQNPTP-----PKLPPSQQSHH---QYQSNL------- 60
           MLQHQIVQSPARLGLT P SPS QNPTP     P    S QS H   Q Q NL       
Sbjct: 1   MLQHQIVQSPARLGLTGPGSPSVQNPTPTRHGHPTSSSSSQSQHQQIQQQPNLLPSSTVA 60

Query: 61  -------SSPTPSSTLLPLLPPLPRAQALLLQMASLASKLFEVYPNRALWLTAFRGSLPT 120
                  S+   SS LL LLPPLPRAQALL QMA L SKLF+V PNRA+WL+AFRGSLP+
Sbjct: 61  AASSASASAAVSSSALLSLLPPLPRAQALLQQMAVLTSKLFDVSPNRAIWLSAFRGSLPS 120

Query: 121 FLNSQTQSAPSTPLDSTPSTSKEILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEMR 180
           FL+S +   P    + +PS++KEILS FN LQTQLFEAV ELQEILDLQD+KQK+A E++
Sbjct: 121 FLSSHSLPPPPPLENPSPSSTKEILSQFNSLQTQLFEAVTELQEILDLQDAKQKVAREIK 180

Query: 181 AKDAALRAFANKLKDAEQVLDVLFDDYADYQGHKRVKTNDDVE---NESLCSTTTVASQL 240
           +KD++L AFANKLKDAE+VLD+L DDY+DY+  KR K  +D E   NES  S+TTV+SQL
Sbjct: 181 SKDSSLLAFANKLKDAERVLDMLVDDYSDYRKPKRSKIEEDDEDNDNESSSSSTTVSSQL 240

Query: 241 KLSDILSYAHKISYTTFAPPEFGAGQAPLRGALPPAPQEEQMRASQLYSFADLDVGLPKS 300
           KL DIL+YAHKISYTTFAPPEFGAGQAPLRGALPPAPQ+EQMRASQLY+FADLD+GLPK+
Sbjct: 241 KLKDILAYAHKISYTTFAPPEFGAGQAPLRGALPPAPQDEQMRASQLYTFADLDIGLPKT 300

Query: 301 VESTEKTIEPMIEAPAPVPTDANPLALAGIQGLLPPNITVPSGWKPGMPVELPRDLPMPP 360
           VE+ EK +E +IE P P       + ++ I  LLPPNI VPSGWKPGMPVELPRDLP+PP
Sbjct: 301 VENMEKKVEALIEPPPP----PEAMDISAIHNLLPPNIAVPSGWKPGMPVELPRDLPLPP 360

Query: 361 PGWKPGDPVPLPPLDSLPVARVDEPQLRHNALPGLHRPQEPIQVRHVQLDILDQDDDSSD 408
           PGWKPGDPV LPPL+S+   R ++ Q       GLHRP + IQVR VQLDIL +DDDSSD
Sbjct: 361 PGWKPGDPVVLPPLESIAAPRAEDHQ-HMRPSQGLHRPPDVIQVRAVQLDIL-EDDDSSD 420

The following BLAST results are available for this feature:

BLAST of CmaCh07G009410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZ00	6.6e-136	64.81	Mediator of RNA polymerase II transcription subunit 4 OS=Arabidopsis thaliana OX...	[more]
Q54D00	1.9e-05	30.69	Putative mediator of RNA polymerase II transcription subunit 4 OS=Dictyostelium ...	[more]

BLAST of CmaCh07G009410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02850.1	4.7e-137	64.81	hydroxyproline-rich glycoprotein family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019258	Mediator complex, subunit Med4	PFAM	PF10018	Med4	coord: 129..265 e-value: 3.3E-7 score: 30.2
IPR019258	Mediator complex, subunit Med4	PANTHER	PTHR13208	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4	coord: 50..404
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 389..407
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..56
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 366..407
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..52
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh07G009410.1	CmaCh07G009410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0070847	core mediator complex
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003712	transcription coregulator activity