CmaCh07G003430 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G003430
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionalkaline ceramidase
LocationCma_Chr07: 1475646 .. 1476886 (+)
RNA-Seq ExpressionCmaCh07G003430
SyntenyCmaCh07G003430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGATGGAATATCAAGTTTCTGGGGTCCTGTCACATCTACAATAGAGTGTTGTGAGAAGAATTATGCTTTTTCTTCTTATATTGCCGAGTTTTATAACACAATATCAAACATCCCAACCGTTCTTTTGGCTCTGATCGGTCTTGCAAATGCTTTGAGACAAGGTTTTGAGAAGAGATTTAGTGTACTTCACATATCAAATATGATACTGGCCATTGGCAGCATGTTCTATCATGCCACATTGCAGAAAGTGTAAGTTCTAATGGCATGCTTTTGGTGTTCTGGTTGCAGTTCTTTCCATTTAGATATTTGAAGGCTGTGTTCTTGAATGTTTGTTCATTACTGAGCTTTAACAACCAACGATCAACTCTTTGTTTTGTAACCAGGCAACAGCAAGGTGATGAAACACCAATGATCTGGGAAATGCTTCTTTACATGTATATTCTCTATTCACCAGATTGGCATTACCGAAGTACGATGCCAACATTCCTCTTCCTATACGGGGCCATTTTTGCTGTGGCTCATTCAATTCTTCGTTATGACATCGGTTTCAAGCTGCACTATGTGATTCTTTGTCTCCTCTGCATACCTCGAATGTATAAGTATTATATATACACAGAAGATGCCTCGGCTAAGCGGTTAGCGAGGCTGTACTTGCTGACTCTTCTCTTAGGCATTTTCTGTTGGGTAATCGATCGGGGTTTCTGTGAGAAGATATCACATTGGACCATCAATCCTCAAGGTCATGCCCTGTGGCATGGTTTCATGGGTTTGAGTTCCTACTTTGCTAACACATTCTTAATGTTTTGCCGGGCGCAGCAAAGAGGATGGGCACCAAAAGTTCTTCATTTAATGGGAATTCTCCCCTACGTGAAGATTGAAAAACCCAAAACCCAGTAAGAAGACACGAATTTGAAGACAAATTATGACAATTTTTGACCTGTGTTGAGTGTACCCTGGAGGTAGAGAAGAAAAAGCATGCAATCTTTTGGGCTATGTTACTTCTTTTCCTTCTTTTCACTGTAAGGAATGAATAGAAACTTAGATTTTTGTAGTACAAATAACAGCAGTTACGTATTATTGTTCATTGACGACAGGGCTTGATTCTCGAGGATTTATGCGTTACAGAGTGACTTAGCATTTAACTCTTTCACAGAGCTGAAGCGCAACATCTGTAAAATATTGCAGTATGTTCAATCTATTTTACTCCCTGTAGTTTTCTTTTAACCTTCTGTGCGC

mRNA sequence

ATGGCTGATGGAATATCAAGTTTCTGGGGTCCTGTCACATCTACAATAGAGTGTTGTGAGAAGAATTATGCTTTTTCTTCTTATATTGCCGAGTTTTATAACACAATATCAAACATCCCAACCGTTCTTTTGGCTCTGATCGGTCTTGCAAATGCTTTGAGACAAGGTTTTGAGAAGAGATTTAGTGTACTTCACATATCAAATATGATACTGGCCATTGGCAGCATGTTCTATCATGCCACATTGCAGAAAGTGCAACAGCAAGGTGATGAAACACCAATGATCTGGGAAATGCTTCTTTACATGTATATTCTCTATTCACCAGATTGGCATTACCGAAGTACGATGCCAACATTCCTCTTCCTATACGGGGCCATTTTTGCTGTGGCTCATTCAATTCTTCGTTATGACATCGGTTTCAAGCTGCACTATGTGATTCTTTGTCTCCTCTGCATACCTCGAATGTATAAGTATTATATATACACAGAAGATGCCTCGGCTAAGCGGTTAGCGAGGCTGTACTTGCTGACTCTTCTCTTAGGCATTTTCTGTTGGGTAATCGATCGGGGTTTCTGTGAGAAGATATCACATTGGACCATCAATCCTCAAGGTCATGCCCTGTGGCATGGTTTCATGGGTTTGAGTTCCTACTTTGCTAACACATTCTTAATGTTTTGCCGGGCGCAGCAAAGAGGATGGGCACCAAAAGTTCTTCATTTAATGGGAATTCTCCCCTACGTGAAGATTGAAAAACCCAAAACCCAGTAAGAAGACACGAATTTGAAGACAAATTATGACAATTTTTGACCTGTGTTGAGTGTACCCTGGAGGTAGAGAAGAAAAAGCATGCAATCTTTTGGGCTATGTTACTTCTTTTCCTTCTTTTCACTGTAAGGAATGAATAGAAACTTAGATTTTTGTAGTACAAATAACAGCAGTTACGTATTATTGTTCATTGACGACAGGGCTTGATTCTCGAGGATTTATGCGTTACAGAGTGACTTAGCATTTAACTCTTTCACAGAGCTGAAGCGCAACATCTGTAAAATATTGCAGTATGTTCAATCTATTTTACTCCCTGTAGTTTTCTTTTAACCTTCTGTGCGC

Coding sequence (CDS)

ATGGCTGATGGAATATCAAGTTTCTGGGGTCCTGTCACATCTACAATAGAGTGTTGTGAGAAGAATTATGCTTTTTCTTCTTATATTGCCGAGTTTTATAACACAATATCAAACATCCCAACCGTTCTTTTGGCTCTGATCGGTCTTGCAAATGCTTTGAGACAAGGTTTTGAGAAGAGATTTAGTGTACTTCACATATCAAATATGATACTGGCCATTGGCAGCATGTTCTATCATGCCACATTGCAGAAAGTGCAACAGCAAGGTGATGAAACACCAATGATCTGGGAAATGCTTCTTTACATGTATATTCTCTATTCACCAGATTGGCATTACCGAAGTACGATGCCAACATTCCTCTTCCTATACGGGGCCATTTTTGCTGTGGCTCATTCAATTCTTCGTTATGACATCGGTTTCAAGCTGCACTATGTGATTCTTTGTCTCCTCTGCATACCTCGAATGTATAAGTATTATATATACACAGAAGATGCCTCGGCTAAGCGGTTAGCGAGGCTGTACTTGCTGACTCTTCTCTTAGGCATTTTCTGTTGGGTAATCGATCGGGGTTTCTGTGAGAAGATATCACATTGGACCATCAATCCTCAAGGTCATGCCCTGTGGCATGGTTTCATGGGTTTGAGTTCCTACTTTGCTAACACATTCTTAATGTTTTGCCGGGCGCAGCAAAGAGGATGGGCACCAAAAGTTCTTCATTTAATGGGAATTCTCCCCTACGTGAAGATTGAAAAACCCAAAACCCAGTAA

Protein sequence

MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKRFSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLLGIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHLMGILPYVKIEKPKTQ
Homology
BLAST of CmaCh07G003430 vs. ExPASy Swiss-Prot
Match: Q94IB9 (Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 5.2e-123
Identity = 203/255 (79.61%), Postives = 226/255 (88.63%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCE NYA+SSYIAEFYNTISN+P +LLALIGL NALRQ FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FS+LHISNMILAIGSM YHATLQ VQQQ DETPM+WE+LLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSILHISNMILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGA FA+ H+ LR+ IGFK+HYVILCLLCIPRMYKYYI+TED +AKR+A+ Y+ T+L+
Sbjct: 121 FLYGAAFAIVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILV 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           G  CW  DR FC+ IS W +NPQGHALWH FM  +SY ANTFLMFCRAQQRGW PKV + 
Sbjct: 181 GSICWFCDRVFCKTISQWPVNPQGHALWHVFMSFNSYCANTFLMFCRAQQRGWNPKVKYF 240

Query: 241 MGILPYVKIEKPKTQ 256
           +G+LPYVKIEKPKTQ
Sbjct: 241 LGVLPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. ExPASy Swiss-Prot
Match: Q9NUN7 (Alkaline ceramidase 3 OS=Homo sapiens OX=9606 GN=ACER3 PE=1 SV=3)

HSP 1 Score: 125.9 bits (315), Expect = 6.3e-28
Identity = 79/258 (30.62%), Postives = 127/258 (49.22%), Query Frame = 0

Query: 8   FWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKRFSVLHIS 67
           +WGP TST++ CE+NY+ + YIAEF+NT+SN+  ++  + G   ++R G EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEKRYIASYLA 69

Query: 68  NMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILY-------SPDWHYRSTMPTFL 127
             ++ +GS  +H TL+   Q  DE PMI+   +++Y ++       S ++H   T    L
Sbjct: 70  LTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHLLFT----L 129

Query: 128 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 187
            L+  I    +  ++  I  ++ Y +L    + R   Y +       + L    L   LL
Sbjct: 130 VLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSI-YIVTWVYPWLRGLGYTSLGIFLL 189

Query: 188 GIFCWVIDRGFCEKISHWT--------INPQGHALWHGFMGLSSYFANTFLMFCRAQQRG 247
           G   W ID  FCE + ++         I  Q HA WH   GL SY    F ++ R     
Sbjct: 190 GFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTLYLR 249

Query: 248 WAPKVLHLMGILPYVKIE 251
           + PKV  L GI P +  E
Sbjct: 250 YRPKVKFLFGIWPVILFE 262

BLAST of CmaCh07G003430 vs. ExPASy Swiss-Prot
Match: Q9D099 (Alkaline ceramidase 3 OS=Mus musculus OX=10090 GN=Acer3 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 4.1e-27
Identity = 76/258 (29.46%), Postives = 124/258 (48.06%), Query Frame = 0

Query: 8   FWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKRFSVLHIS 67
           +WGP TST++ CE+NY  + ++AEF+NT+SN+  ++  + G    +R   EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYVVTLFVAEFWNTVSNLIMIIPPIFGAIQGIRDRLEKRYIAAYLA 69

Query: 68  NMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILY-------SPDWHYRSTMPTFL 127
             ++ +GS  +H TL+   Q  DE PMI+   +++Y ++       S ++H   T    L
Sbjct: 70  LTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKTKSSINYHLLFT----L 129

Query: 128 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 187
           FLY       +  ++  I  ++ Y +L    + R   Y +       + L    L   LL
Sbjct: 130 FLYSLTVTTIYLKVKEPIFHQVMYGMLVFTLVLRSI-YIVTWVYPWLRGLGYTSLTVFLL 189

Query: 188 GIFCWVIDRGFCEKISHWT--------INPQGHALWHGFMGLSSYFANTFLMFCRAQQRG 247
           G   W ID  FC+ + ++         +  Q HA WH   GL SY    F ++ R     
Sbjct: 190 GFLLWNIDNIFCDSLRNFRKRVPPVLGVTTQFHAWWHILTGLGSYLHILFSLYTRTLYLR 249

Query: 248 WAPKVLHLMGILPYVKIE 251
           + PKV  L GI P V  E
Sbjct: 250 YRPKVKFLFGIWPAVMFE 262

BLAST of CmaCh07G003430 vs. ExPASy Swiss-Prot
Match: Q6TMJ1 (Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum OX=44689 GN=dcd3A PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 6.4e-20
Identity = 75/273 (27.47%), Postives = 124/273 (45.42%), Query Frame = 0

Query: 9   WGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQ---GFEK------ 68
           +G  T++I+ CE NY +S YIAEFYNT S++   L  + G+   +     G EK      
Sbjct: 10  YGTPTASIDWCELNYTYSPYIAEFYNTFSSLIISLFGIYGIWIMMPNFGTGVEKEHIKIL 69

Query: 69  -------RFSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWH- 128
                  +  + +IS +++ +GS FYHATL    Q  DE PMI+  L+ +YI+ +     
Sbjct: 70  KQLDVRNKVILSYISLIVVGVGSAFYHATLLYQNQLFDELPMIYTALIMLYIMVTVGEEK 129

Query: 129 --------------YRSTMPTFLFLYGAIFAVA-------HSILRYDIGFKLHYVILCLL 188
                          R  +P  L  YG    +          IL+   G  + YV+   +
Sbjct: 130 TKKGFKGGVLGNSLLRHLLPYLLIAYGLFVTITILVIQDQPKILQVSFGALVFYVVFHSI 189

Query: 189 CIPRMYKYYIYTEDASAKRLARLYLLTLLLGIFCWVIDRGFCEKISHWTINPQGHALWHG 244
            +    K      +  +  L +   +++L+G  CWV++R FC+  +  T   Q HA WH 
Sbjct: 190 YLINKKKPDGMPSNPDS-YLYKYAFVSMLVGFTCWVVERYFCK--NGKTFGFQLHAFWHF 249

BLAST of CmaCh07G003430 vs. ExPASy Swiss-Prot
Match: Q9VIP7 (Alkaline ceramidase OS=Drosophila melanogaster OX=7227 GN=bwa PE=2 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 5.6e-16
Identity = 68/219 (31.05%), Postives = 110/219 (50.23%), Query Frame = 0

Query: 11  PVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLL--ALIGLANALRQGFEKRFSVLHISN 70
           P +S ++ CE NY  SS IAEF NT SN   +LL   LI L     +       V+ +  
Sbjct: 22  PGSSPVDWCEGNYLISSNIAEFVNTFSNFLFILLPPVLIMLFKEYGRFVTPGIHVIWVLL 81

Query: 71  MILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHY----RSTMPTF--LFL 130
           +++ + SM++HATL  + Q  DE  ++W + +  + L+ P  +Y    ++   TF  L L
Sbjct: 82  IVVGLSSMYFHATLSLIGQLLDELAILW-VFMAAFSLFYPKRYYPKFVKNDRKTFSWLML 141

Query: 131 YGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTL---L 190
             AI A   S  +  +    +  +L  + +P M   Y   +  S +R+ RL + +     
Sbjct: 142 LSAIAATGLSWWKPIV----NAFVLMFMSVPTMVMLYTELQRVSDQRVYRLGIRSTTVWA 201

Query: 191 LGIFCWVIDRGFCEKISHWTIN-PQGHALWHGFMGLSSY 218
           + +FCW+ DR FCE  S  +IN P  H  WH F+ +++Y
Sbjct: 202 VAVFCWINDRIFCEAWS--SINFPYLHGFWHIFIFIAAY 233

BLAST of CmaCh07G003430 vs. ExPASy TrEMBL
Match: A0A6J1KSV2 (alkaline ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111497288 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.4e-147
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. ExPASy TrEMBL
Match: A0A6J1EME1 (alkaline ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111433883 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.0e-145
Identity = 251/255 (98.43%), Postives = 253/255 (99.22%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCEKNYA+SSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           GIFCWVIDR  CEKISHW+INPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRCLCEKISHWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. ExPASy TrEMBL
Match: A0A6J1BUA9 (alkaline ceramidase OS=Momordica charantia OX=3673 GN=LOC111005428 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.4e-139
Identity = 238/255 (93.33%), Postives = 248/255 (97.25%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTST+ECCEKNYA+S+Y+AEF+NTISNIPT+LLALIGL+NAL+Q FEKR
Sbjct: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQK+QQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGAIFAVAHSILRY IGFK+HYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTL L
Sbjct: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           GI CWVIDRGFCEKISHW INPQGHALWH FMGL SYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. ExPASy TrEMBL
Match: A0A1S3B0Z4 (alkaline ceramidase 3 OS=Cucumis melo OX=3656 GN=LOC103484652 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.2e-139
Identity = 238/255 (93.33%), Postives = 247/255 (96.86%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTST ECCE+NYA+SSYIAEFYNT+SNIPT+LLALIGLANALRQ FEKR
Sbjct: 1   MADGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGA+FAVAHSILRYDIGFK+HYVILCLLCIPRMYKYYIYTEDASAKRLARLY LTLLL
Sbjct: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           G FCWV DRG CEKISHW INPQGHALWH FMGLSSY+ANTFLMFCRAQQRGW+PKVLHL
Sbjct: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MG+LPYVKIEKPKTQ
Sbjct: 241 MGVLPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. ExPASy TrEMBL
Match: A0A1R3I2Q7 (Ceramidase OS=Corchorus capsularis OX=210143 GN=CCACVL1_15312 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 7.3e-128
Identity = 218/255 (85.49%), Postives = 235/255 (92.16%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCEKNYA+SSYIAEFYNTISNIPTVLLALIGL NALRQ FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLVNALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLH+SNMILAIGSM YHATLQ+VQQQ DETPM+WEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHVSNMILAIGSMLYHATLQRVQQQSDETPMVWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGA+FAV HS++R+ IGFK+HYVILCLLCIPRMYKYYI+TEDASAKRLA+ Y+ TL L
Sbjct: 121 FLYGAVFAVVHSVIRFGIGFKVHYVILCLLCIPRMYKYYIHTEDASAKRLAKWYVATLSL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           G  CW  DR FC++IS W INPQGHALWH FMG + YFANTFLMFCRAQQRGWAPKV++ 
Sbjct: 181 GSLCWFCDRVFCKQISSWPINPQGHALWHFFMGFNCYFANTFLMFCRAQQRGWAPKVVYF 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. NCBI nr
Match: XP_023003815.1 (alkaline ceramidase [Cucurbita maxima] >XP_023003816.1 alkaline ceramidase [Cucurbita maxima] >XP_023003817.1 alkaline ceramidase [Cucurbita maxima] >XP_023003819.1 alkaline ceramidase [Cucurbita maxima] >XP_023003820.1 alkaline ceramidase [Cucurbita maxima])

HSP 1 Score: 531.9 bits (1369), Expect = 2.9e-147
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. NCBI nr
Match: KAG7026660.1 (Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 525.8 bits (1353), Expect = 2.1e-145
Identity = 252/255 (98.82%), Postives = 254/255 (99.61%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCEKNYA+SSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           GIFCWVIDR FCEKISHW+INPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRCFCEKISHWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. NCBI nr
Match: XP_023517286.1 (alkaline ceramidase [Cucurbita pepo subsp. pepo] >XP_023517287.1 alkaline ceramidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 524.6 bits (1350), Expect = 4.7e-145
Identity = 251/255 (98.43%), Postives = 254/255 (99.61%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTED+SAKRLARLYLLTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDSSAKRLARLYLLTLLL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           GIFCWVIDR FC+KISHW+INPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRCFCDKISHWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. NCBI nr
Match: XP_022926905.1 (alkaline ceramidase [Cucurbita moschata] >XP_022926906.1 alkaline ceramidase [Cucurbita moschata] >XP_022926907.1 alkaline ceramidase [Cucurbita moschata] >XP_022926908.1 alkaline ceramidase [Cucurbita moschata])

HSP 1 Score: 523.5 bits (1347), Expect = 1.0e-144
Identity = 251/255 (98.43%), Postives = 253/255 (99.22%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCEKNYA+SSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           GIFCWVIDR  CEKISHW+INPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRCLCEKISHWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. NCBI nr
Match: KAG6594691.1 (Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 523.5 bits (1347), Expect = 1.0e-144
Identity = 251/255 (98.43%), Postives = 254/255 (99.61%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCEKNYA+SSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           GIFCWVIDR FCEKIS+W+INPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL
Sbjct: 181 GIFCWVIDRCFCEKISYWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGILPYVKIEKPKTQ 256
           MGILPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

BLAST of CmaCh07G003430 vs. TAIR 10
Match: AT4G22330.1 (Alkaline phytoceramidase (aPHC) )

HSP 1 Score: 441.8 bits (1135), Expect = 3.7e-124
Identity = 203/255 (79.61%), Postives = 226/255 (88.63%), Query Frame = 0

Query: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60
           MADGISSFWGPVTSTIECCE NYA+SSYIAEFYNTISN+P +LLALIGL NALRQ FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FS+LHISNMILAIGSM YHATLQ VQQQ DETPM+WE+LLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSILHISNMILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180
           FLYGA FA+ H+ LR+ IGFK+HYVILCLLCIPRMYKYYI+TED +AKR+A+ Y+ T+L+
Sbjct: 121 FLYGAAFAIVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILV 180

Query: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240
           G  CW  DR FC+ IS W +NPQGHALWH FM  +SY ANTFLMFCRAQQRGW PKV + 
Sbjct: 181 GSICWFCDRVFCKTISQWPVNPQGHALWHVFMSFNSYCANTFLMFCRAQQRGWNPKVKYF 240

Query: 241 MGILPYVKIEKPKTQ 256
           +G+LPYVKIEKPKTQ
Sbjct: 241 LGVLPYVKIEKPKTQ 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94IB95.2e-12379.61Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1[more]
Q9NUN76.3e-2830.62Alkaline ceramidase 3 OS=Homo sapiens OX=9606 GN=ACER3 PE=1 SV=3[more]
Q9D0994.1e-2729.46Alkaline ceramidase 3 OS=Mus musculus OX=10090 GN=Acer3 PE=1 SV=1[more]
Q6TMJ16.4e-2027.47Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum OX=44689 GN=dcd3A... [more]
Q9VIP75.6e-1631.05Alkaline ceramidase OS=Drosophila melanogaster OX=7227 GN=bwa PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KSV21.4e-147100.00alkaline ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111497288 PE=3 SV=1[more]
A0A6J1EME15.0e-14598.43alkaline ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111433883 PE=3 SV=1[more]
A0A6J1BUA92.4e-13993.33alkaline ceramidase OS=Momordica charantia OX=3673 GN=LOC111005428 PE=3 SV=1[more]
A0A1S3B0Z43.2e-13993.33alkaline ceramidase 3 OS=Cucumis melo OX=3656 GN=LOC103484652 PE=3 SV=1[more]
A0A1R3I2Q77.3e-12885.49Ceramidase OS=Corchorus capsularis OX=210143 GN=CCACVL1_15312 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023003815.12.9e-147100.00alkaline ceramidase [Cucurbita maxima] >XP_023003816.1 alkaline ceramidase [Cucu... [more]
KAG7026660.12.1e-14598.82Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023517286.14.7e-14598.43alkaline ceramidase [Cucurbita pepo subsp. pepo] >XP_023517287.1 alkaline cerami... [more]
XP_022926905.11.0e-14498.43alkaline ceramidase [Cucurbita moschata] >XP_022926906.1 alkaline ceramidase [Cu... [more]
KAG6594691.11.0e-14498.43Alkaline ceramidase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT4G22330.13.7e-12479.61Alkaline phytoceramidase (aPHC) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008901Alkaline ceramidasePFAMPF05875Ceramidasecoord: 8..238
e-value: 3.7E-57
score: 193.7
NoneNo IPR availablePANTHERPTHR46852:SF5ALKALINE CERAMIDASEcoord: 1..255
IPR044219Alkaline ceramidase, plantPANTHERPTHR46852ALKALINE CERAMIDASEcoord: 1..255

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G003430.1CmaCh07G003430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006672 ceramide metabolic process
biological_process GO:0009651 response to salt stress
biological_process GO:0006914 autophagy
biological_process GO:0010025 wax biosynthetic process
biological_process GO:0030104 water homeostasis
biological_process GO:0042742 defense response to bacterium
biological_process GO:0030148 sphingolipid biosynthetic process
biological_process GO:0031667 response to nutrient levels
biological_process GO:0002238 response to molecule of fungal origin
biological_process GO:0009737 response to abscisic acid
biological_process GO:0090333 regulation of stomatal closure
biological_process GO:0010508 positive regulation of autophagy
biological_process GO:0010150 leaf senescence
biological_process GO:0098542 defense response to other organism
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides